KR102729768B1 - 트랜스제닉 선택 방법 및 조성물 - Google Patents

트랜스제닉 선택 방법 및 조성물 Download PDF

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KR102729768B1
KR102729768B1 KR1020207013411A KR20207013411A KR102729768B1 KR 102729768 B1 KR102729768 B1 KR 102729768B1 KR 1020207013411 A KR1020207013411 A KR 1020207013411A KR 20207013411 A KR20207013411 A KR 20207013411A KR 102729768 B1 KR102729768 B1 KR 102729768B1
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intein
terminal fragment
protein
molecule
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KR20200064129A (ko
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앨버트 쳉
나다니엘 질레트
메건 두
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더 잭슨 래보라토리
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3517—Marker; Tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
KR1020207013411A 2017-10-12 2018-10-11 트랜스제닉 선택 방법 및 조성물 Active KR102729768B1 (ko)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201762571672P 2017-10-12 2017-10-12
US62/571,672 2017-10-12
US201762608478P 2017-12-20 2017-12-20
US62/608,478 2017-12-20
US201862616281P 2018-01-11 2018-01-11
US62/616,281 2018-01-11
US201862624629P 2018-01-31 2018-01-31
US62/624,629 2018-01-31
PCT/US2018/055412 WO2019075200A1 (en) 2017-10-12 2018-10-11 METHODS AND COMPOSITIONS OF TRANSGENIC SELECTION

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KR20200064129A KR20200064129A (ko) 2020-06-05
KR102729768B1 true KR102729768B1 (ko) 2024-11-14

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US (1) US20200263197A1 (https=)
EP (1) EP3694869A4 (https=)
JP (2) JP7394752B2 (https=)
KR (1) KR102729768B1 (https=)
CN (1) CN111511759B (https=)
AU (1) AU2018347421B2 (https=)
CA (1) CA3079017A1 (https=)
WO (1) WO2019075200A1 (https=)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11293021B1 (en) 2016-06-23 2022-04-05 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
JP2020530979A (ja) 2017-06-30 2020-11-05 インスクリプタ， インコーポレイテッド 自動細胞処理方法、モジュール、機器及びシステム
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
CA3108767A1 (en) 2018-06-30 2020-01-02 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US11214781B2 (en) 2018-10-22 2022-01-04 Inscripta, Inc. Engineered enzyme
US11001831B2 (en) * 2019-03-25 2021-05-11 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
AU2020247900A1 (en) 2019-03-25 2021-11-04 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
AU2020288623A1 (en) 2019-06-06 2022-01-06 Inscripta, Inc. Curing for recursive nucleic acid-guided cell editing
US11203762B2 (en) 2019-11-19 2021-12-21 Inscripta, Inc. Methods for increasing observed editing in bacteria
AU2020407048A1 (en) 2019-12-18 2022-06-09 Inscripta, Inc. Cascade/dCas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells
US11225674B2 (en) 2020-01-27 2022-01-18 Inscripta, Inc. Electroporation modules and instrumentation
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
US11299731B1 (en) 2020-09-15 2022-04-12 Inscripta, Inc. CRISPR editing to embed nucleic acid landing pads into genomes of live cells
US11512297B2 (en) 2020-11-09 2022-11-29 Inscripta, Inc. Affinity tag for recombination protein recruitment
AU2021415461A1 (en) 2021-01-04 2023-08-17 Inscripta, Inc. Mad nucleases
EP4274890A4 (en) 2021-01-07 2024-11-27 Inscripta, Inc. MAD-NUCLEASES
US11884924B2 (en) 2021-02-16 2024-01-30 Inscripta, Inc. Dual strand nucleic acid-guided nickase editing
US20240124850A1 (en) 2021-03-03 2024-04-18 Shape Therapeutics Inc. Auxotrophic Cells for Virus Production and Compositions and Methods of Making
WO2023027170A1 (ja) * 2021-08-27 2023-03-02 国立大学法人 東京医科歯科大学 タンパク質の翻訳制御システム
WO2023027169A1 (ja) * 2021-08-27 2023-03-02 国立大学法人 東京医科歯科大学 生細胞の選別システム
CN116063546B (zh) * 2021-10-29 2026-01-16 中科辅酶医科技(深圳)有限公司 一种基于共振能量转移的全遗传编码nad+蛋白探针及其制备方法和应用
EP4473123A4 (en) * 2022-02-01 2026-03-11 Shape Therapeutics Inc STABLE CELL LINES FOR INDUCIBLE VAAR VIRION PRODUCTION
CN114686440B (zh) * 2022-05-31 2022-09-27 南京艾尔普再生医学科技有限公司 一种低免疫原性iPSC细胞的制备方法、低免疫原性iPSC细胞及组合物
CN115896147B (zh) * 2022-10-11 2023-10-03 态创生物科技(广州)有限公司 内含肽进化系统和方法、对应的突变质粒和报告质粒
WO2024216202A2 (en) * 2023-04-13 2024-10-17 The Regents Of The University Of California Fret biosensor recombinant proteins
US20250154517A1 (en) * 2023-09-22 2025-05-15 Ut-Battelle, Llc System for plant co-transformation and methods of use
CN120923600A (zh) * 2024-05-09 2025-11-11 福建医科大学 一种超稳定红色荧光蛋白
WO2026044087A1 (en) 2024-08-21 2026-02-26 Shape Therapeutics Inc. Increased cellular stability for aav production
WO2026073032A1 (en) 2024-09-27 2026-04-02 Shape Therapeutics Inc. Constructs for improved aav production

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000071701A1 (en) 1999-05-24 2000-11-30 New England Biolabs, Inc. Method for generating split, non-transferable genes that are able to express an active protein product
US6858775B1 (en) * 1999-05-24 2005-02-22 New England Biolabs, Inc. Method for generating split, non-transferable genes that are able to express an active protein product

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10113174A (ja) * 1996-10-08 1998-05-06 Amashiyamu Kk ヒトシトクロムｐ−４５０とヒトシトクロムｐ−４５０還元酵素の同時製造方法
EP2395084B1 (en) * 2002-01-08 2018-03-14 Agrivida, Inc. Transgenic plants expressing CIVPS or intein modified proteins and related method
AU2004315485A1 (en) * 2003-10-24 2005-08-18 The Regents Of The University Of California Self-assembling split-fluorescent protein systems
MX2008000985A (es) * 2005-07-21 2008-04-07 Abbott Lab Expresion de gen multiple que incluye construcciones y metodos sorf (cuadro de lectura abierta simple) con poliproteinas, pro-proteinas y proteolisis.
JP5801207B2 (ja) * 2009-02-27 2015-10-28 ノバルティス アーゲー ２種類の選択マーカーを含む発現ベクター系
KR102096534B1 (ko) * 2011-09-28 2020-04-03 에라 바이오테크, 에스.에이. 분할된 인테인 및 그의 이용

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000071701A1 (en) 1999-05-24 2000-11-30 New England Biolabs, Inc. Method for generating split, non-transferable genes that are able to express an active protein product
US6858775B1 (en) * 1999-05-24 2005-02-22 New England Biolabs, Inc. Method for generating split, non-transferable genes that are able to express an active protein product

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ACS Synth Biol.,1(10):445-450(2012.9.4.)
Biotechnology Letters.,37(11):2121-2137(2015.7.8.)

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EP3694869A1 (en) 2020-08-19
JP2024015079A (ja) 2024-02-01
KR20200064129A (ko) 2020-06-05
US20200263197A1 (en) 2020-08-20
CA3079017A1 (en) 2019-04-18
WO2019075200A1 (en) 2019-04-18
CN111511759B (zh) 2024-07-30
CN111511759A (zh) 2020-08-07
JP7394752B2 (ja) 2023-12-08
AU2018347421B2 (en) 2024-08-22
AU2018347421A1 (en) 2020-05-14
JP2020537646A (ja) 2020-12-24
EP3694869A4 (en) 2021-11-24

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