CA3079017A1 - Transgenic selection methods and compositions - Google Patents
Transgenic selection methods and compositions Download PDFInfo
- Publication number
- CA3079017A1 CA3079017A1 CA3079017A CA3079017A CA3079017A1 CA 3079017 A1 CA3079017 A1 CA 3079017A1 CA 3079017 A CA3079017 A CA 3079017A CA 3079017 A CA3079017 A CA 3079017A CA 3079017 A1 CA3079017 A1 CA 3079017A1
- Authority
- CA
- Canada
- Prior art keywords
- terminal fragment
- intein
- protein
- nucleotide sequence
- sequence encoding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
- C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3517—Marker; Tag
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3519—Fusion with another nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16041—Use of virus, viral particle or viral elements as a vector
- C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762571672P | 2017-10-12 | 2017-10-12 | |
| US62/571,672 | 2017-10-12 | ||
| US201762608478P | 2017-12-20 | 2017-12-20 | |
| US62/608,478 | 2017-12-20 | ||
| US201862616281P | 2018-01-11 | 2018-01-11 | |
| US62/616,281 | 2018-01-11 | ||
| US201862624629P | 2018-01-31 | 2018-01-31 | |
| US62/624,629 | 2018-01-31 | ||
| PCT/US2018/055412 WO2019075200A1 (en) | 2017-10-12 | 2018-10-11 | METHODS AND COMPOSITIONS OF TRANSGENIC SELECTION |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3079017A1 true CA3079017A1 (en) | 2019-04-18 |
Family
ID=66101179
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3079017A Pending CA3079017A1 (en) | 2017-10-12 | 2018-10-11 | Transgenic selection methods and compositions |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20200263197A1 (https=) |
| EP (1) | EP3694869A4 (https=) |
| JP (2) | JP7394752B2 (https=) |
| KR (1) | KR102729768B1 (https=) |
| CN (1) | CN111511759B (https=) |
| AU (1) | AU2018347421B2 (https=) |
| CA (1) | CA3079017A1 (https=) |
| WO (1) | WO2019075200A1 (https=) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11293021B1 (en) | 2016-06-23 | 2022-04-05 | Inscripta, Inc. | Automated cell processing methods, modules, instruments, and systems |
| US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| JP2020530979A (ja) | 2017-06-30 | 2020-11-05 | インスクリプタ， インコーポレイテッド | 自動細胞処理方法、モジュール、機器及びシステム |
| US10557216B2 (en) | 2018-04-24 | 2020-02-11 | Inscripta, Inc. | Automated instrumentation for production of T-cell receptor peptide libraries |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| CA3108767A1 (en) | 2018-06-30 | 2020-01-02 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| US11214781B2 (en) | 2018-10-22 | 2022-01-04 | Inscripta, Inc. | Engineered enzyme |
| US11001831B2 (en) * | 2019-03-25 | 2021-05-11 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| AU2020247900A1 (en) | 2019-03-25 | 2021-11-04 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| AU2020288623A1 (en) | 2019-06-06 | 2022-01-06 | Inscripta, Inc. | Curing for recursive nucleic acid-guided cell editing |
| US11203762B2 (en) | 2019-11-19 | 2021-12-21 | Inscripta, Inc. | Methods for increasing observed editing in bacteria |
| AU2020407048A1 (en) | 2019-12-18 | 2022-06-09 | Inscripta, Inc. | Cascade/dCas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells |
| US11225674B2 (en) | 2020-01-27 | 2022-01-18 | Inscripta, Inc. | Electroporation modules and instrumentation |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
| US11299731B1 (en) | 2020-09-15 | 2022-04-12 | Inscripta, Inc. | CRISPR editing to embed nucleic acid landing pads into genomes of live cells |
| US11512297B2 (en) | 2020-11-09 | 2022-11-29 | Inscripta, Inc. | Affinity tag for recombination protein recruitment |
| AU2021415461A1 (en) | 2021-01-04 | 2023-08-17 | Inscripta, Inc. | Mad nucleases |
| EP4274890A4 (en) | 2021-01-07 | 2024-11-27 | Inscripta, Inc. | MAD-NUCLEASES |
| US11884924B2 (en) | 2021-02-16 | 2024-01-30 | Inscripta, Inc. | Dual strand nucleic acid-guided nickase editing |
| US20240124850A1 (en) | 2021-03-03 | 2024-04-18 | Shape Therapeutics Inc. | Auxotrophic Cells for Virus Production and Compositions and Methods of Making |
| WO2023027170A1 (ja) * | 2021-08-27 | 2023-03-02 | 国立大学法人 東京医科歯科大学 | タンパク質の翻訳制御システム |
| WO2023027169A1 (ja) * | 2021-08-27 | 2023-03-02 | 国立大学法人 東京医科歯科大学 | 生細胞の選別システム |
| CN116063546B (zh) * | 2021-10-29 | 2026-01-16 | 中科辅酶医科技(深圳)有限公司 | 一种基于共振能量转移的全遗传编码nad+蛋白探针及其制备方法和应用 |
| EP4473123A4 (en) * | 2022-02-01 | 2026-03-11 | Shape Therapeutics Inc | STABLE CELL LINES FOR INDUCIBLE VAAR VIRION PRODUCTION |
| CN114686440B (zh) * | 2022-05-31 | 2022-09-27 | 南京艾尔普再生医学科技有限公司 | 一种低免疫原性iPSC细胞的制备方法、低免疫原性iPSC细胞及组合物 |
| CN115896147B (zh) * | 2022-10-11 | 2023-10-03 | 态创生物科技(广州)有限公司 | 内含肽进化系统和方法、对应的突变质粒和报告质粒 |
| WO2024216202A2 (en) * | 2023-04-13 | 2024-10-17 | The Regents Of The University Of California | Fret biosensor recombinant proteins |
| US20250154517A1 (en) * | 2023-09-22 | 2025-05-15 | Ut-Battelle, Llc | System for plant co-transformation and methods of use |
| CN120923600A (zh) * | 2024-05-09 | 2025-11-11 | 福建医科大学 | 一种超稳定红色荧光蛋白 |
| WO2026044087A1 (en) | 2024-08-21 | 2026-02-26 | Shape Therapeutics Inc. | Increased cellular stability for aav production |
| WO2026073032A1 (en) | 2024-09-27 | 2026-04-02 | Shape Therapeutics Inc. | Constructs for improved aav production |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10113174A (ja) * | 1996-10-08 | 1998-05-06 | Amashiyamu Kk | ヒトシトクロムｐ−４５０とヒトシトクロムｐ−４５０還元酵素の同時製造方法 |
| US6858775B1 (en) * | 1999-05-24 | 2005-02-22 | New England Biolabs, Inc. | Method for generating split, non-transferable genes that are able to express an active protein product |
| DE60036942T2 (de) * | 1999-05-24 | 2008-08-07 | New England Biolabs, Inc., Beverly | Verfahren zur herstellung von getrennten, nicht-übertragbaren genen, welche befähigt sind ein aktives proteinprodukt zu exprimieren |
| EP2395084B1 (en) * | 2002-01-08 | 2018-03-14 | Agrivida, Inc. | Transgenic plants expressing CIVPS or intein modified proteins and related method |
| AU2004315485A1 (en) * | 2003-10-24 | 2005-08-18 | The Regents Of The University Of California | Self-assembling split-fluorescent protein systems |
| MX2008000985A (es) * | 2005-07-21 | 2008-04-07 | Abbott Lab | Expresion de gen multiple que incluye construcciones y metodos sorf (cuadro de lectura abierta simple) con poliproteinas, pro-proteinas y proteolisis. |
| JP5801207B2 (ja) * | 2009-02-27 | 2015-10-28 | ノバルティス アーゲー | ２種類の選択マーカーを含む発現ベクター系 |
| KR102096534B1 (ko) * | 2011-09-28 | 2020-04-03 | 에라 바이오테크, 에스.에이. | 분할된 인테인 및 그의 이용 |
-
2018
- 2018-10-11 KR KR1020207013411A patent/KR102729768B1/ko active Active
- 2018-10-11 US US16/755,065 patent/US20200263197A1/en active Pending
- 2018-10-11 AU AU2018347421A patent/AU2018347421B2/en active Active
- 2018-10-11 CA CA3079017A patent/CA3079017A1/en active Pending
- 2018-10-11 EP EP18867279.4A patent/EP3694869A4/en active Pending
- 2018-10-11 CN CN201880078542.7A patent/CN111511759B/zh active Active
- 2018-10-11 WO PCT/US2018/055412 patent/WO2019075200A1/en not_active Ceased
- 2018-10-11 JP JP2020520468A patent/JP7394752B2/ja active Active
-
2023
- 2023-11-28 JP JP2023200808A patent/JP2024015079A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3694869A1 (en) | 2020-08-19 |
| JP2024015079A (ja) | 2024-02-01 |
| KR20200064129A (ko) | 2020-06-05 |
| US20200263197A1 (en) | 2020-08-20 |
| WO2019075200A1 (en) | 2019-04-18 |
| CN111511759B (zh) | 2024-07-30 |
| CN111511759A (zh) | 2020-08-07 |
| JP7394752B2 (ja) | 2023-12-08 |
| AU2018347421B2 (en) | 2024-08-22 |
| AU2018347421A1 (en) | 2020-05-14 |
| KR102729768B1 (ko) | 2024-11-14 |
| JP2020537646A (ja) | 2020-12-24 |
| EP3694869A4 (en) | 2021-11-24 |
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