JP7394752B2 - トランスジェニック選択方法および組成物 - Google Patents

トランスジェニック選択方法および組成物 Download PDF

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JP7394752B2
JP7394752B2 JP2020520468A JP2020520468A JP7394752B2 JP 7394752 B2 JP7394752 B2 JP 7394752B2 JP 2020520468 A JP2020520468 A JP 2020520468A JP 2020520468 A JP2020520468 A JP 2020520468A JP 7394752 B2 JP7394752 B2 JP 7394752B2
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molecule
interest
intein
terminal fragment
transgene
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JP2020537646A5 (https=
JP2020537646A (ja
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アルバート チェン，
ナサニエル ジレット，
メンハン ドゥ，
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Jackson Laboratory
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Jackson Laboratory
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3517—Marker; Tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
JP2020520468A 2017-10-12 2018-10-11 トランスジェニック選択方法および組成物 Active JP7394752B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2023200808A JP2024015079A (ja) 2017-10-12 2023-11-28 トランスジェニック選択方法および組成物

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201762571672P 2017-10-12 2017-10-12
US62/571,672 2017-10-12
US201762608478P 2017-12-20 2017-12-20
US62/608,478 2017-12-20
US201862616281P 2018-01-11 2018-01-11
US62/616,281 2018-01-11
US201862624629P 2018-01-31 2018-01-31
US62/624,629 2018-01-31
PCT/US2018/055412 WO2019075200A1 (en) 2017-10-12 2018-10-11 METHODS AND COMPOSITIONS OF TRANSGENIC SELECTION

Related Child Applications (1)

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JP2023200808A Division JP2024015079A (ja) 2017-10-12 2023-11-28 トランスジェニック選択方法および組成物

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JP2020537646A JP2020537646A (ja) 2020-12-24
JP2020537646A5 JP2020537646A5 (https=) 2021-11-25
JP7394752B2 true JP7394752B2 (ja) 2023-12-08

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JP2023200808A Pending JP2024015079A (ja) 2017-10-12 2023-11-28 トランスジェニック選択方法および組成物

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US (1) US20200263197A1 (https=)
EP (1) EP3694869A4 (https=)
JP (2) JP7394752B2 (https=)
KR (1) KR102729768B1 (https=)
CN (1) CN111511759B (https=)
AU (1) AU2018347421B2 (https=)
CA (1) CA3079017A1 (https=)
WO (1) WO2019075200A1 (https=)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11293021B1 (en) 2016-06-23 2022-04-05 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
JP2020530979A (ja) 2017-06-30 2020-11-05 インスクリプタ， インコーポレイテッド 自動細胞処理方法、モジュール、機器及びシステム
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
CA3108767A1 (en) 2018-06-30 2020-01-02 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US11214781B2 (en) 2018-10-22 2022-01-04 Inscripta, Inc. Engineered enzyme
US11001831B2 (en) * 2019-03-25 2021-05-11 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
AU2020247900A1 (en) 2019-03-25 2021-11-04 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
AU2020288623A1 (en) 2019-06-06 2022-01-06 Inscripta, Inc. Curing for recursive nucleic acid-guided cell editing
US11203762B2 (en) 2019-11-19 2021-12-21 Inscripta, Inc. Methods for increasing observed editing in bacteria
AU2020407048A1 (en) 2019-12-18 2022-06-09 Inscripta, Inc. Cascade/dCas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells
US11225674B2 (en) 2020-01-27 2022-01-18 Inscripta, Inc. Electroporation modules and instrumentation
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
US11299731B1 (en) 2020-09-15 2022-04-12 Inscripta, Inc. CRISPR editing to embed nucleic acid landing pads into genomes of live cells
US11512297B2 (en) 2020-11-09 2022-11-29 Inscripta, Inc. Affinity tag for recombination protein recruitment
AU2021415461A1 (en) 2021-01-04 2023-08-17 Inscripta, Inc. Mad nucleases
EP4274890A4 (en) 2021-01-07 2024-11-27 Inscripta, Inc. MAD-NUCLEASES
US11884924B2 (en) 2021-02-16 2024-01-30 Inscripta, Inc. Dual strand nucleic acid-guided nickase editing
US20240124850A1 (en) 2021-03-03 2024-04-18 Shape Therapeutics Inc. Auxotrophic Cells for Virus Production and Compositions and Methods of Making
WO2023027170A1 (ja) * 2021-08-27 2023-03-02 国立大学法人 東京医科歯科大学 タンパク質の翻訳制御システム
WO2023027169A1 (ja) * 2021-08-27 2023-03-02 国立大学法人 東京医科歯科大学 生細胞の選別システム
CN116063546B (zh) * 2021-10-29 2026-01-16 中科辅酶医科技(深圳)有限公司 一种基于共振能量转移的全遗传编码nad+蛋白探针及其制备方法和应用
EP4473123A4 (en) * 2022-02-01 2026-03-11 Shape Therapeutics Inc STABLE CELL LINES FOR INDUCIBLE VAAR VIRION PRODUCTION
CN114686440B (zh) * 2022-05-31 2022-09-27 南京艾尔普再生医学科技有限公司 一种低免疫原性iPSC细胞的制备方法、低免疫原性iPSC细胞及组合物
CN115896147B (zh) * 2022-10-11 2023-10-03 态创生物科技(广州)有限公司 内含肽进化系统和方法、对应的突变质粒和报告质粒
WO2024216202A2 (en) * 2023-04-13 2024-10-17 The Regents Of The University Of California Fret biosensor recombinant proteins
US20250154517A1 (en) * 2023-09-22 2025-05-15 Ut-Battelle, Llc System for plant co-transformation and methods of use
CN120923600A (zh) * 2024-05-09 2025-11-11 福建医科大学 一种超稳定红色荧光蛋白
WO2026044087A1 (en) 2024-08-21 2026-02-26 Shape Therapeutics Inc. Increased cellular stability for aav production
WO2026073032A1 (en) 2024-09-27 2026-04-02 Shape Therapeutics Inc. Constructs for improved aav production

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003505012A (ja) 1999-05-24 2003-02-12 ニュー・イングランド・バイオラブズ・インコーポレイティッド 活性なタンパク質産生物を発現し得る分断した非伝達性遺伝子を産生させるための方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10113174A (ja) * 1996-10-08 1998-05-06 Amashiyamu Kk ヒトシトクロムｐ−４５０とヒトシトクロムｐ−４５０還元酵素の同時製造方法
US6858775B1 (en) * 1999-05-24 2005-02-22 New England Biolabs, Inc. Method for generating split, non-transferable genes that are able to express an active protein product
EP2395084B1 (en) * 2002-01-08 2018-03-14 Agrivida, Inc. Transgenic plants expressing CIVPS or intein modified proteins and related method
AU2004315485A1 (en) * 2003-10-24 2005-08-18 The Regents Of The University Of California Self-assembling split-fluorescent protein systems
MX2008000985A (es) * 2005-07-21 2008-04-07 Abbott Lab Expresion de gen multiple que incluye construcciones y metodos sorf (cuadro de lectura abierta simple) con poliproteinas, pro-proteinas y proteolisis.
JP5801207B2 (ja) * 2009-02-27 2015-10-28 ノバルティス アーゲー ２種類の選択マーカーを含む発現ベクター系
KR102096534B1 (ko) * 2011-09-28 2020-04-03 에라 바이오테크, 에스.에이. 분할된 인테인 및 그의 이용

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003505012A (ja) 1999-05-24 2003-02-12 ニュー・イングランド・バイオラブズ・インコーポレイティッド 活性なタンパク質産生物を発現し得る分断した非伝達性遺伝子を産生させるための方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ACS Synth. Biol.，2012年，Vol.1，p.445-450
Mol. BioSyst.，2010年，DOI:10.1039/c0mb00034e

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Publication number Publication date
EP3694869A1 (en) 2020-08-19
JP2024015079A (ja) 2024-02-01
KR20200064129A (ko) 2020-06-05
US20200263197A1 (en) 2020-08-20
CA3079017A1 (en) 2019-04-18
WO2019075200A1 (en) 2019-04-18
CN111511759B (zh) 2024-07-30
CN111511759A (zh) 2020-08-07
AU2018347421B2 (en) 2024-08-22
AU2018347421A1 (en) 2020-05-14
KR102729768B1 (ko) 2024-11-14
JP2020537646A (ja) 2020-12-24
EP3694869A4 (en) 2021-11-24

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