KR102530821B1 - 단일 단계 다중 표적화를 통한 표적화된 유전자 변형을 위한 방법 및 조성물 - Google Patents

단일 단계 다중 표적화를 통한 표적화된 유전자 변형을 위한 방법 및 조성물 Download PDF

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KR102530821B1
KR102530821B1 KR1020177019826A KR20177019826A KR102530821B1 KR 102530821 B1 KR102530821 B1 KR 102530821B1 KR 1020177019826 A KR1020177019826 A KR 1020177019826A KR 20177019826 A KR20177019826 A KR 20177019826A KR 102530821 B1 KR102530821 B1 KR 102530821B1
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베라 보로니나
린 맥도날드
브라이언 잠브로비츠
앤드류 제이. 머피
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리제너론 파마슈티칼스 인코포레이티드
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine

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KR1020177019826A 2014-12-19 2015-12-18 단일 단계 다중 표적화를 통한 표적화된 유전자 변형을 위한 방법 및 조성물 Active KR102530821B1 (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201462094104P 2014-12-19 2014-12-19
US62/094,104 2014-12-19
US201562167408P 2015-05-28 2015-05-28
US62/167,408 2015-05-28
US201562205524P 2015-08-14 2015-08-14
US62/205,524 2015-08-14
PCT/US2015/066681 WO2016100819A1 (en) 2014-12-19 2015-12-18 Methods and compositions for targeted genetic modification through single-step multiple targeting

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KR20170093246A KR20170093246A (ko) 2017-08-14
KR102530821B1 true KR102530821B1 (ko) 2023-05-10

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EP (2) EP3653048B9 (enExample)
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CN (1) CN107208113A (enExample)
AU (1) AU2015364427B2 (enExample)
BR (1) BR112017013104A2 (enExample)
CA (1) CA2971213C (enExample)
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NZ (1) NZ732895A (enExample)
RU (1) RU2707137C2 (enExample)
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