MX388784B - Métodos y composiciones para la modificación genética dirigida a través de direccionamiento múltiple de una sola etapa. - Google Patents
Métodos y composiciones para la modificación genética dirigida a través de direccionamiento múltiple de una sola etapa.Info
- Publication number
- MX388784B MX388784B MX2017008190A MX2017008190A MX388784B MX 388784 B MX388784 B MX 388784B MX 2017008190 A MX2017008190 A MX 2017008190A MX 2017008190 A MX2017008190 A MX 2017008190A MX 388784 B MX388784 B MX 388784B
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- compositions
- genomic locus
- targeted genetic
- genetic modification
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0276—Knock-out vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Environmental Sciences (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462094104P | 2014-12-19 | 2014-12-19 | |
| US201562167408P | 2015-05-28 | 2015-05-28 | |
| US201562205524P | 2015-08-14 | 2015-08-14 | |
| PCT/US2015/066681 WO2016100819A1 (en) | 2014-12-19 | 2015-12-18 | Methods and compositions for targeted genetic modification through single-step multiple targeting |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2017008190A MX2017008190A (es) | 2018-03-23 |
| MX388784B true MX388784B (es) | 2025-03-20 |
Family
ID=55229831
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2017008190A MX388784B (es) | 2014-12-19 | 2015-12-18 | Métodos y composiciones para la modificación genética dirigida a través de direccionamiento múltiple de una sola etapa. |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US11326184B2 (enExample) |
| EP (2) | EP3653048B9 (enExample) |
| JP (2) | JP6840077B2 (enExample) |
| KR (1) | KR102530821B1 (enExample) |
| CN (1) | CN107208113A (enExample) |
| AU (1) | AU2015364427B2 (enExample) |
| BR (1) | BR112017013104A2 (enExample) |
| CA (1) | CA2971213C (enExample) |
| ES (2) | ES2947714T3 (enExample) |
| IL (1) | IL252755B (enExample) |
| MX (1) | MX388784B (enExample) |
| NZ (1) | NZ732895A (enExample) |
| RU (1) | RU2707137C2 (enExample) |
| SG (1) | SG11201704646YA (enExample) |
| WO (1) | WO2016100819A1 (enExample) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2645475C2 (ru) | 2012-04-25 | 2018-02-21 | Регенерон Фармасьютикалз, Инк. | Опосредованное нуклеазой нацеливание с большими нацеливающими векторами |
| DK2986729T3 (en) | 2013-04-16 | 2018-10-29 | Regeneron Pharma | TARGETED MODIFICATION OF ROOT THROUGH |
| WO2015088643A1 (en) | 2013-12-11 | 2015-06-18 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for the targeted modification of a genome |
| RU2704283C9 (ru) | 2014-06-06 | 2020-02-07 | Регенерон Фармасьютикалз, Инк. | Способы и композиции для модификации целевого локуса |
| PT3161128T (pt) | 2014-06-26 | 2018-11-21 | Regeneron Pharma | Métodos e composições para modificações genéticas alvejadas e métodos de utilização |
| AU2015330699B2 (en) | 2014-10-10 | 2021-12-02 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| SG11201702309RA (en) | 2014-10-15 | 2017-04-27 | Regeneron Pharma | Methods and compositions for generating or maintaining pluripotent cells |
| WO2016073990A2 (en) | 2014-11-07 | 2016-05-12 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
| CA2968440A1 (en) | 2014-11-21 | 2016-05-26 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for targeted genetic modification using paired guide rnas |
| BR112017013104A2 (en) | 2014-12-19 | 2018-05-15 | Regeneron Pharmaceuticals, Inc. | methods for modifying a target genomic locus in a cell, for enhancing homologous recombination at a target genomic locus in a cell, and for producing an f0 generation from a nonhuman animal. |
| EP3689139B1 (en) | 2015-05-29 | 2025-01-01 | Regeneron Pharmaceuticals, Inc. | Rodent cells having a disruption in a c9orf72 locus |
| EP3353296B1 (en) | 2015-09-24 | 2020-11-04 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
| IL302725A (en) | 2016-01-13 | 2023-07-01 | Regeneron Pharma | Rodents having an engineered heavy chain diversity region |
| US11597924B2 (en) | 2016-03-25 | 2023-03-07 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| WO2017180694A1 (en) | 2016-04-13 | 2017-10-19 | Editas Medicine, Inc. | Cas9 fusion molecules gene editing systems, and methods of use thereof |
| EP3518667B1 (en) | 2016-09-30 | 2024-10-30 | Regeneron Pharmaceuticals, Inc. | Rodents having a hexanucleotide repeat expansion in a c9orf72 locus |
| PT4082334T (pt) | 2016-11-04 | 2025-05-21 | Regeneron Pharma | Animais não humanos possuindo um lócus da cadeia leve lambda de immunoglobulina geneticamente modificada |
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| WO2019014564A1 (en) | 2017-07-14 | 2019-01-17 | Editas Medicine, Inc. | SYSTEMS AND METHODS OF TARGETED INTEGRATION AND GENOME EDITING AND DETECTION THEREOF WITH INTEGRATED PRIMING SITES |
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| TW202438676A (zh) | 2017-12-05 | 2024-10-01 | 美商再生元醫藥公司 | 具有經改造免疫球蛋白λ輕鏈的非人類動物及其用途 |
| IL318469A (en) | 2018-06-14 | 2025-03-01 | Regeneron Pharma | Non-human animals capable of reorganizing transgenic DH-DH, and their uses |
| CN111321171A (zh) * | 2018-12-14 | 2020-06-23 | 江苏集萃药康生物科技有限公司 | 一种应用CRISPR/Cas9介导ES打靶技术制备基因打靶动物模型的方法 |
| US11690362B2 (en) | 2018-12-20 | 2023-07-04 | Regeneran Pharmaceuticals, Inc. | Nuclease-mediated repeat expansion |
| AU2020253531C1 (en) * | 2019-04-04 | 2022-06-16 | Regeneron Pharmaceuticals, Inc. | Methods for scarless introduction of targeted modifications into targeting vectors |
| SG11202111258TA (en) | 2019-06-05 | 2021-11-29 | Regeneron Pharma | Non-human animals having a limited lambda light chain repertoire expressed from the kappa locus and uses thereof |
| US20240317849A1 (en) | 2020-12-23 | 2024-09-26 | Regeneron Pharmaceuticals, Inc. | Nucleic acids encoding anchor modified antibodies and uses thereof |
| TW202332770A (zh) * | 2021-09-24 | 2023-08-16 | 大陸商上海伊米諾康生物科技有限公司 | 大尺寸染色體轉移方法及使用該方法產生的經修飾的染色體和生物體 |
| KR20250112318A (ko) * | 2022-10-27 | 2025-07-23 | 씨울프 테라퓨틱스, 인코포레이티드 | 핵 표적 dna 전달 및 이를 실시하는데 사용하기 위한 조성물 |
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2015
- 2015-12-18 BR BR112017013104-8A patent/BR112017013104A2/en not_active Application Discontinuation
- 2015-12-18 SG SG11201704646YA patent/SG11201704646YA/en unknown
- 2015-12-18 CN CN201580069507.5A patent/CN107208113A/zh active Pending
- 2015-12-18 EP EP19201384.5A patent/EP3653048B9/en active Active
- 2015-12-18 CA CA2971213A patent/CA2971213C/en active Active
- 2015-12-18 JP JP2017532808A patent/JP6840077B2/ja active Active
- 2015-12-18 ES ES19201384T patent/ES2947714T3/es active Active
- 2015-12-18 ES ES15828604T patent/ES2760508T3/es active Active
- 2015-12-18 RU RU2017124909A patent/RU2707137C2/ru active
- 2015-12-18 AU AU2015364427A patent/AU2015364427B2/en active Active
- 2015-12-18 MX MX2017008190A patent/MX388784B/es unknown
- 2015-12-18 WO PCT/US2015/066681 patent/WO2016100819A1/en not_active Ceased
- 2015-12-18 KR KR1020177019826A patent/KR102530821B1/ko active Active
- 2015-12-18 US US14/974,623 patent/US11326184B2/en active Active
- 2015-12-18 NZ NZ732895A patent/NZ732895A/en unknown
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2017
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2022
- 2022-04-05 US US17/713,696 patent/US12553064B2/en active Active
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