KR102516695B1 - 단백질 내로의 비-천연 아미노산의 혼입 - Google Patents

단백질 내로의 비-천연 아미노산의 혼입 Download PDF

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KR102516695B1
KR102516695B1 KR1020177014048A KR20177014048A KR102516695B1 KR 102516695 B1 KR102516695 B1 KR 102516695B1 KR 1020177014048 A KR1020177014048 A KR 1020177014048A KR 20177014048 A KR20177014048 A KR 20177014048A KR 102516695 B1 KR102516695 B1 KR 102516695B1
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KR20170070238A (ko
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제이슨 더블류. 친
울프강 에이치. 슈미드
시몬 제이. 엘사서
유진 와이. 킴
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유나이티드 킹덤 리서치 앤드 이노베이션
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/01026—Pyrrolysine-tRNAPyl ligase (6.1.1.26)

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  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
KR1020177014048A 2014-10-27 2015-10-21 단백질 내로의 비-천연 아미노산의 혼입 Active KR102516695B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1419109.2A GB201419109D0 (en) 2014-10-27 2014-10-27 Incorporation of unnatural amino acids into proteins
GB1419109.2 2014-10-27
PCT/GB2015/053141 WO2016066995A1 (en) 2014-10-27 2015-10-21 Incorporation of unnatural amino acids into proteins

Publications (2)

Publication Number Publication Date
KR20170070238A KR20170070238A (ko) 2017-06-21
KR102516695B1 true KR102516695B1 (ko) 2023-03-30

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US (1) US10738339B2 (enExample)
EP (1) EP3212799B1 (enExample)
JP (2) JP6843044B2 (enExample)
KR (1) KR102516695B1 (enExample)
CN (1) CN107002088B (enExample)
BR (1) BR112017008108A2 (enExample)
CA (1) CA2965085A1 (enExample)
GB (1) GB201419109D0 (enExample)
WO (1) WO2016066995A1 (enExample)

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JP6343604B2 (ja) 2012-05-18 2018-06-13 メディカル リサーチ カウンシル ＢＣＮ基を含むアミノ酸をコードする直交性コドンと、直交性ＰｙｌＲＳ合成酵素とを使用して、ＢＣＮ基を含むアミノ酸をポリペプチドに取り込む方法
GB2528227A (en) 2014-03-14 2016-01-20 Medical Res Council Cyclopropene amino acids and methods
WO2018044143A1 (ko) * 2016-09-05 2018-03-08 한국과학기술원 목적 단백질의 위치 특이적 변형이 시공간적으로 조절되는 마우스, 이의 제조방법 및 그 용도
CN106834349B (zh) * 2017-02-28 2019-12-13 中国人民解放军军事医学科学院 一种安全性提高的病毒基因递送系统
CN117286182A (zh) * 2017-06-02 2023-12-26 Ambrx公司 促进含非天然氨基酸的蛋白质产生的方法和组合物
CN119410679A (zh) * 2018-05-01 2025-02-11 Ambrx公司 一种用于优化抗体表达的方法
US20210356377A1 (en) * 2018-09-07 2021-11-18 Medimmune, Llc Methods Of Cell Selection
GB201817444D0 (en) 2018-10-26 2018-12-12 Res & Innovation Uk Methods and compositions
EP3696189A1 (en) * 2019-02-14 2020-08-19 European Molecular Biology Laboratory Means and methods for preparing engineered target proteins by genetic code expansion in a target protein selective manner
CN111718949B (zh) * 2019-03-19 2021-10-01 宁波鲲鹏生物科技有限公司 利用双质粒系统在蛋白中引入非天然氨基酸
CN111850020B (zh) * 2019-04-25 2021-05-07 苏州鲲鹏生物技术有限公司 利用质粒系统在蛋白中引入非天然氨基酸
CN111849929B (zh) * 2019-04-30 2021-05-11 苏州鲲鹏生物技术有限公司 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶
GB201906775D0 (en) 2019-05-14 2019-06-26 Res & Innovation Uk Synthetic genome
TW202128996A (zh) * 2019-10-10 2021-08-01 美商史基普研究協會 用於活體內合成非天然多肽的組合物及方法
CN117881779A (zh) 2021-05-28 2024-04-12 英国研究与创新署 微生物及其用途
CN113481239A (zh) * 2021-07-01 2021-10-08 四川大学华西医院 一种通过Rosa26位点向细胞系中引入非天然氨基酸编码体系的方法及其细胞系
AU2022306337A1 (en) * 2021-07-07 2024-02-01 Ajinomoto Co., Inc. Method for secretory production of unnatural-amino-acid-containing protein
GB202110137D0 (en) 2021-07-14 2021-08-25 Res & Innovation Uk Methods for optimising protein production
CN113699124B (zh) * 2021-09-08 2022-04-12 北京大学 一种含非天然氨基酸蛋白的制备方法
EP4504926A2 (en) * 2022-04-08 2025-02-12 ABSCI Corporation Trna synthetases and methods of use thereof
WO2024199465A1 (en) * 2023-03-30 2024-10-03 The Chinese University Of Hong Kong Unnatural amino acid in cellulo synthesis for site-specific protein modification
CN120752342A (zh) * 2023-07-13 2025-10-03 深圳华大生命科学研究院 工程生物遏制系统及方法
CN120210144A (zh) * 2023-12-25 2025-06-27 康码（上海）生物科技有限公司 一种改造的氨酰-tRNA合成酶、核酸构建物以及基因工程菌株

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US20120077186A1 (en) 2010-05-07 2012-03-29 Oregon Health & Science University Use of cysteine-derived suppressor trnas for non-native amino acid incorporation

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BRPI0417801A (pt) * 2003-12-18 2007-04-10 Scripps Research Inst incorporação seletiva de 5-hidroxitriptofano em proteìnas de células mamìferas
AU2005330514B2 (en) * 2004-10-27 2011-05-12 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
CA2706889C (en) 2007-09-20 2018-08-28 Riken Mutant pyrrolysyl-trna synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same
GB2470770A (en) 2009-06-04 2010-12-08 Medical Res Council Incorporation of unnatural amino acids having an orthogonal functional group into polypeptides using orthogonal tRNA/tRNA synthetase pairs
GB201201100D0 (en) 2012-01-20 2012-03-07 Medical Res Council Polypeptides and methods

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BR112017008108A2 (pt) 2017-12-19
US10738339B2 (en) 2020-08-11
WO2016066995A1 (en) 2016-05-06
KR20170070238A (ko) 2017-06-21
CA2965085A1 (en) 2016-05-06
CN107002088B (zh) 2022-01-11
JP6843044B2 (ja) 2021-03-17
GB201419109D0 (en) 2014-12-10
EP3212799B1 (en) 2019-04-17
CN107002088A (zh) 2017-08-01
JP2017532043A (ja) 2017-11-02
US20170356023A1 (en) 2017-12-14
JP2021035374A (ja) 2021-03-04
EP3212799A1 (en) 2017-09-06

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