BR112017008108A2 - incorporação de aminoácidos não naturais em proteínas - Google Patents
incorporação de aminoácidos não naturais em proteínasInfo
- Publication number
- BR112017008108A2 BR112017008108A2 BR112017008108A BR112017008108A BR112017008108A2 BR 112017008108 A2 BR112017008108 A2 BR 112017008108A2 BR 112017008108 A BR112017008108 A BR 112017008108A BR 112017008108 A BR112017008108 A BR 112017008108A BR 112017008108 A2 BR112017008108 A2 BR 112017008108A2
- Authority
- BR
- Brazil
- Prior art keywords
- unnatural amino
- amino acid
- interest
- trna
- sequence
- Prior art date
Links
- 150000001413 amino acids Chemical class 0.000 title abstract 6
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 102000004169 proteins and genes Human genes 0.000 title abstract 5
- 238000010348 incorporation Methods 0.000 title abstract 3
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 4
- 101150073803 ERF1 gene Proteins 0.000 abstract 3
- 101100301666 Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) prf1 gene Proteins 0.000 abstract 3
- 101100033735 Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) prf11 gene Proteins 0.000 abstract 3
- 101100033738 Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) prf12 gene Proteins 0.000 abstract 3
- 150000007523 nucleic acids Chemical group 0.000 abstract 3
- 102000052866 Amino Acyl-tRNA Synthetases Human genes 0.000 abstract 2
- 108700028939 Amino Acyl-tRNA Synthetases Proteins 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 108020004705 Codon Proteins 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
- C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
- C12Y601/01026—Pyrrolysine-tRNAPyl ligase (6.1.1.26)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1419109.2A GB201419109D0 (en) | 2014-10-27 | 2014-10-27 | Incorporation of unnatural amino acids into proteins |
| PCT/GB2015/053141 WO2016066995A1 (en) | 2014-10-27 | 2015-10-21 | Incorporation of unnatural amino acids into proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112017008108A2 true BR112017008108A2 (pt) | 2017-12-19 |
Family
ID=52103474
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112017008108A BR112017008108A2 (pt) | 2014-10-27 | 2015-10-21 | incorporação de aminoácidos não naturais em proteínas |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US10738339B2 (enExample) |
| EP (1) | EP3212799B1 (enExample) |
| JP (2) | JP6843044B2 (enExample) |
| KR (1) | KR102516695B1 (enExample) |
| CN (1) | CN107002088B (enExample) |
| BR (1) | BR112017008108A2 (enExample) |
| CA (1) | CA2965085A1 (enExample) |
| GB (1) | GB201419109D0 (enExample) |
| WO (1) | WO2016066995A1 (enExample) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6343604B2 (ja) | 2012-05-18 | 2018-06-13 | メディカル リサーチ カウンシル | ＢＣＮ基を含むアミノ酸をコードする直交性コドンと、直交性ＰｙｌＲＳ合成酵素とを使用して、ＢＣＮ基を含むアミノ酸をポリペプチドに取り込む方法 |
| GB2528227A (en) | 2014-03-14 | 2016-01-20 | Medical Res Council | Cyclopropene amino acids and methods |
| WO2018044143A1 (ko) * | 2016-09-05 | 2018-03-08 | 한국과학기술원 | 목적 단백질의 위치 특이적 변형이 시공간적으로 조절되는 마우스, 이의 제조방법 및 그 용도 |
| CN106834349B (zh) * | 2017-02-28 | 2019-12-13 | 中国人民解放军军事医学科学院 | 一种安全性提高的病毒基因递送系统 |
| CN117286182A (zh) * | 2017-06-02 | 2023-12-26 | Ambrx公司 | 促进含非天然氨基酸的蛋白质产生的方法和组合物 |
| CN119410679A (zh) * | 2018-05-01 | 2025-02-11 | Ambrx公司 | 一种用于优化抗体表达的方法 |
| US20210356377A1 (en) * | 2018-09-07 | 2021-11-18 | Medimmune, Llc | Methods Of Cell Selection |
| GB201817444D0 (en) | 2018-10-26 | 2018-12-12 | Res & Innovation Uk | Methods and compositions |
| EP3696189A1 (en) * | 2019-02-14 | 2020-08-19 | European Molecular Biology Laboratory | Means and methods for preparing engineered target proteins by genetic code expansion in a target protein selective manner |
| CN111718949B (zh) * | 2019-03-19 | 2021-10-01 | 宁波鲲鹏生物科技有限公司 | 利用双质粒系统在蛋白中引入非天然氨基酸 |
| CN111850020B (zh) * | 2019-04-25 | 2021-05-07 | 苏州鲲鹏生物技术有限公司 | 利用质粒系统在蛋白中引入非天然氨基酸 |
| CN111849929B (zh) * | 2019-04-30 | 2021-05-11 | 苏州鲲鹏生物技术有限公司 | 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 |
| GB201906775D0 (en) | 2019-05-14 | 2019-06-26 | Res & Innovation Uk | Synthetic genome |
| TW202128996A (zh) * | 2019-10-10 | 2021-08-01 | 美商史基普研究協會 | 用於活體內合成非天然多肽的組合物及方法 |
| CN117881779A (zh) | 2021-05-28 | 2024-04-12 | 英国研究与创新署 | 微生物及其用途 |
| CN113481239A (zh) * | 2021-07-01 | 2021-10-08 | 四川大学华西医院 | 一种通过Rosa26位点向细胞系中引入非天然氨基酸编码体系的方法及其细胞系 |
| AU2022306337A1 (en) * | 2021-07-07 | 2024-02-01 | Ajinomoto Co., Inc. | Method for secretory production of unnatural-amino-acid-containing protein |
| GB202110137D0 (en) | 2021-07-14 | 2021-08-25 | Res & Innovation Uk | Methods for optimising protein production |
| CN113699124B (zh) * | 2021-09-08 | 2022-04-12 | 北京大学 | 一种含非天然氨基酸蛋白的制备方法 |
| EP4504926A2 (en) * | 2022-04-08 | 2025-02-12 | ABSCI Corporation | Trna synthetases and methods of use thereof |
| WO2024199465A1 (en) * | 2023-03-30 | 2024-10-03 | The Chinese University Of Hong Kong | Unnatural amino acid in cellulo synthesis for site-specific protein modification |
| CN120752342A (zh) * | 2023-07-13 | 2025-10-03 | 深圳华大生命科学研究院 | 工程生物遏制系统及方法 |
| CN120210144A (zh) * | 2023-12-25 | 2025-06-27 | 康码（上海）生物科技有限公司 | 一种改造的氨酰-tRNA合成酶、核酸构建物以及基因工程菌株 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BRPI0417801A (pt) * | 2003-12-18 | 2007-04-10 | Scripps Research Inst | incorporação seletiva de 5-hidroxitriptofano em proteìnas de células mamìferas |
| AU2005330514B2 (en) * | 2004-10-27 | 2011-05-12 | The Scripps Research Institute | Orthogonal translation components for the in vivo incorporation of unnatural amino acids |
| CA2706889C (en) | 2007-09-20 | 2018-08-28 | Riken | Mutant pyrrolysyl-trna synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same |
| GB2470770A (en) | 2009-06-04 | 2010-12-08 | Medical Res Council | Incorporation of unnatural amino acids having an orthogonal functional group into polypeptides using orthogonal tRNA/tRNA synthetase pairs |
| US20120077186A1 (en) | 2010-05-07 | 2012-03-29 | Oregon Health & Science University | Use of cysteine-derived suppressor trnas for non-native amino acid incorporation |
| GB201201100D0 (en) | 2012-01-20 | 2012-03-07 | Medical Res Council | Polypeptides and methods |
-
2014
- 2014-10-27 GB GBGB1419109.2A patent/GB201419109D0/en not_active Ceased
-
2015
- 2015-10-21 EP EP15794622.9A patent/EP3212799B1/en active Active
- 2015-10-21 US US15/521,103 patent/US10738339B2/en active Active
- 2015-10-21 JP JP2017519813A patent/JP6843044B2/ja active Active
- 2015-10-21 CN CN201580058784.6A patent/CN107002088B/zh active Active
- 2015-10-21 BR BR112017008108A patent/BR112017008108A2/pt not_active Application Discontinuation
- 2015-10-21 WO PCT/GB2015/053141 patent/WO2016066995A1/en not_active Ceased
- 2015-10-21 CA CA2965085A patent/CA2965085A1/en not_active Abandoned
- 2015-10-21 KR KR1020177014048A patent/KR102516695B1/ko active Active
-
2020
- 2020-10-22 JP JP2020177605A patent/JP2021035374A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| US10738339B2 (en) | 2020-08-11 |
| WO2016066995A1 (en) | 2016-05-06 |
| KR20170070238A (ko) | 2017-06-21 |
| CA2965085A1 (en) | 2016-05-06 |
| KR102516695B1 (ko) | 2023-03-30 |
| CN107002088B (zh) | 2022-01-11 |
| JP6843044B2 (ja) | 2021-03-17 |
| GB201419109D0 (en) | 2014-12-10 |
| EP3212799B1 (en) | 2019-04-17 |
| CN107002088A (zh) | 2017-08-01 |
| JP2017532043A (ja) | 2017-11-02 |
| US20170356023A1 (en) | 2017-12-14 |
| JP2021035374A (ja) | 2021-03-04 |
| EP3212799A1 (en) | 2017-09-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B25A | Requested transfer of rights approved |
Owner name: UNITED KINGDOM RESEARCH AND INNOVATION (GB) |
|
| B11A | Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing | ||
| B11Y | Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette] |