CA2965085A1 - Incorporation of unnatural amino acids into proteins - Google Patents

Incorporation of unnatural amino acids into proteins Download PDF

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Publication number
CA2965085A1
CA2965085A1 CA2965085A CA2965085A CA2965085A1 CA 2965085 A1 CA2965085 A1 CA 2965085A1 CA 2965085 A CA2965085 A CA 2965085A CA 2965085 A CA2965085 A CA 2965085A CA 2965085 A1 CA2965085 A1 CA 2965085A1
Authority
CA
Canada
Prior art keywords
amino acid
erf1
unnatural amino
nucleic acid
trna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2965085A
Other languages
English (en)
French (fr)
Inventor
Jason W. Chin
Wolfgang H. SCHMIED
Simon J. ELSASSER
Eugene Y. KYM
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
United Kingdom Research and Innovation
Original Assignee
Medical Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Research Council filed Critical Medical Research Council
Publication of CA2965085A1 publication Critical patent/CA2965085A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/01026—Pyrrolysine-tRNAPyl ligase (6.1.1.26)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CA2965085A 2014-10-27 2015-10-21 Incorporation of unnatural amino acids into proteins Abandoned CA2965085A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1419109.2A GB201419109D0 (en) 2014-10-27 2014-10-27 Incorporation of unnatural amino acids into proteins
GB1419109.2 2014-10-27
PCT/GB2015/053141 WO2016066995A1 (en) 2014-10-27 2015-10-21 Incorporation of unnatural amino acids into proteins

Publications (1)

Publication Number Publication Date
CA2965085A1 true CA2965085A1 (en) 2016-05-06

Family

ID=52103474

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2965085A Abandoned CA2965085A1 (en) 2014-10-27 2015-10-21 Incorporation of unnatural amino acids into proteins

Country Status (9)

Country Link
US (1) US10738339B2 (enExample)
EP (1) EP3212799B1 (enExample)
JP (2) JP6843044B2 (enExample)
KR (1) KR102516695B1 (enExample)
CN (1) CN107002088B (enExample)
BR (1) BR112017008108A2 (enExample)
CA (1) CA2965085A1 (enExample)
GB (1) GB201419109D0 (enExample)
WO (1) WO2016066995A1 (enExample)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2852664B1 (en) 2012-05-18 2017-09-06 Medical Research Council Methods of incorporating an amino acid comprising a bcn group into a polypeptide using an orthogonal codon encoding it and an orthorgonal pylrs synthase.
GB2528227A (en) 2014-03-14 2016-01-20 Medical Res Council Cyclopropene amino acids and methods
WO2018044143A1 (ko) * 2016-09-05 2018-03-08 한국과학기술원 목적 단백질의 위치 특이적 변형이 시공간적으로 조절되는 마우스, 이의 제조방법 및 그 용도
CN106834349B (zh) * 2017-02-28 2019-12-13 中国人民解放军军事医学科学院 一种安全性提高的病毒基因递送系统
KR102705871B1 (ko) 2017-06-02 2024-09-11 암브룩스, 인코포레이티드 비천연 아미노산 함유 단백질 생성을 촉진하는 방법 및 조성물
CA3098910A1 (en) * 2018-05-01 2019-11-07 Ambrx, Inc. A method for optimizing antibody expression
US20210356377A1 (en) * 2018-09-07 2021-11-18 Medimmune, Llc Methods Of Cell Selection
GB201817444D0 (en) 2018-10-26 2018-12-12 Res & Innovation Uk Methods and compositions
EP3696189A1 (en) * 2019-02-14 2020-08-19 European Molecular Biology Laboratory Means and methods for preparing engineered target proteins by genetic code expansion in a target protein selective manner
CN111718949B (zh) * 2019-03-19 2021-10-01 宁波鲲鹏生物科技有限公司 利用双质粒系统在蛋白中引入非天然氨基酸
CN111850020B (zh) * 2019-04-25 2021-05-07 苏州鲲鹏生物技术有限公司 利用质粒系统在蛋白中引入非天然氨基酸
CN111849929B (zh) * 2019-04-30 2021-05-11 苏州鲲鹏生物技术有限公司 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶
GB201906775D0 (en) 2019-05-14 2019-06-26 Res & Innovation Uk Synthetic genome
TW202128996A (zh) * 2019-10-10 2021-08-01 美商史基普研究協會 用於活體內合成非天然多肽的組合物及方法
CN117881779A (zh) 2021-05-28 2024-04-12 英国研究与创新署 微生物及其用途
CN113481239A (zh) * 2021-07-01 2021-10-08 四川大学华西医院 一种通过Rosa26位点向细胞系中引入非天然氨基酸编码体系的方法及其细胞系
AU2022306337A1 (en) * 2021-07-07 2024-02-01 Ajinomoto Co., Inc. Method for secretory production of unnatural-amino-acid-containing protein
GB202110137D0 (en) 2021-07-14 2021-08-25 Res & Innovation Uk Methods for optimising protein production
CN113699124B (zh) * 2021-09-08 2022-04-12 北京大学 一种含非天然氨基酸蛋白的制备方法
US20230407363A1 (en) * 2022-04-08 2023-12-21 Absci Corporation tRNA SYNTHETASES AND METHODS OF USE THEREOF
EP4689125A1 (en) * 2023-03-30 2026-02-11 The Chinese University of Hong Kong Unnatural amino acid in cellulo synthesis for site-specific protein modification
WO2025010715A1 (zh) * 2023-07-13 2025-01-16 深圳华大生命科学研究院 工程生物遏制系统及方法
CN120210144A (zh) * 2023-12-25 2025-06-27 康码（上海）生物科技有限公司 一种改造的氨酰-tRNA合成酶、核酸构建物以及基因工程菌株

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2549830A1 (en) * 2003-12-18 2006-01-05 The Scripps Research Institute Selective incorporation of 5-hyroxytryptophan into proteins in mammalian cells
MX2007004892A (es) * 2004-10-27 2007-08-16 Scripps Research Inst Componentes de traduccion ortogonales para la incorporacion in vivo de aminoacidos no naturales.
CN101952428B (zh) 2007-09-20 2013-10-23 独立行政法人理化学研究所 突变体吡咯赖氨酰-tRNA合成酶以及使用该酶的非天然氨基酸整合蛋白质的制造方法
GB2470770A (en) 2009-06-04 2010-12-08 Medical Res Council Incorporation of unnatural amino acids having an orthogonal functional group into polypeptides using orthogonal tRNA/tRNA synthetase pairs
US20120077186A1 (en) * 2010-05-07 2012-03-29 Oregon Health & Science University Use of cysteine-derived suppressor trnas for non-native amino acid incorporation
GB201201100D0 (en) 2012-01-20 2012-03-07 Medical Res Council Polypeptides and methods

Also Published As

Publication number Publication date
BR112017008108A2 (pt) 2017-12-19
EP3212799A1 (en) 2017-09-06
JP6843044B2 (ja) 2021-03-17
JP2017532043A (ja) 2017-11-02
KR20170070238A (ko) 2017-06-21
US10738339B2 (en) 2020-08-11
CN107002088B (zh) 2022-01-11
US20170356023A1 (en) 2017-12-14
CN107002088A (zh) 2017-08-01
JP2021035374A (ja) 2021-03-04
KR102516695B1 (ko) 2023-03-30
GB201419109D0 (en) 2014-12-10
EP3212799B1 (en) 2019-04-17
WO2016066995A1 (en) 2016-05-06

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Legal Events

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FZDE Discontinued

Effective date: 20191022