KR102506624B1 - Fruity floral Abies koreana aroma composition for enhancing concentration of human brain - Google Patents

Abstract

The present invention relates to a fragrance composition for increasing the concentration of the human brain, a method for producing a health functional food and cosmetic composition for increasing the concentration of the human brain containing the fragrance composition, a fragrance mixture for increasing the concentration of the human brain, and a human brain prepared by the method The fragrance composition of the present invention induces an increase in AHB and RHB in the left frontal lobe and an increase in RMB and SEF50 in the left temporal lobe, thereby enhancing the concentration of the brain.

Description

Fruity floral Abies koreana aroma composition for enhancing concentration of human brain}

The present invention relates to a fragrance composition for increasing the concentration of the human brain, a method for producing a health functional food and cosmetic composition for increasing the concentration of the human brain containing the fragrance composition, a fragrance mixture for increasing the concentration of the human brain, and a human brain prepared by the method It relates to a fragrance mixture for increasing the concentration of

Perfume is a product obtained by diluting essential oils, a raw material for volatile fragrance compounds, with ethanol. Natural flavors are divided into animal flavors obtained from animals and plant flavors obtained from plants. Animal spices include ambergris from humpback whales, musk from civet deer, civette from civet cats, and castorium from beavers, but these days they are not used well. Plant spices can be obtained by distilling, extracting, or pressing flowers, leaves, fruits, seeds, resins, roots, and stems of about 1,500 species of plants, but their purity is low, and their fragrance changes greatly during extraction and distillation. There are severe changes in strength and quality depending on the region, harvest time, and post-harvest storage method, and since supply uncertainty is large, about 200 species are currently on the market.

Organic synthetic fragrances can be obtained through organic synthesis in the laboratory. They are more pure than natural fragrances, are cheaper to use, stable, have a longer shelf life, have a higher concentration, and are preferred in the fragrance industry because there is no problem with seasonal supply. . A representative example of organic synthetic perfume is aldehyde used in Chanel No.5.

Each of the spices represents a unique and unique fragrance, which is called a fragrance or note. Fragrance notes are generally citrus, floral, green, fruity, aldehyde, oriental, spicy, woody, cypre, and after. It is represented by fougere, marine, and gourmand. When several spices are mixed, the scent of each spice changes. Combining several single spice products is called blending, and through this, a combination spice product with various fragrances can be created. Combination spices are divided into fragrance (aromatic flavoring) and flavor (food flavoring) according to their use. Depending on the content of essential oils, fragrances are classified into perfume (15-30%), eau de parfum (15-18%), eau de toilette (4-8%), and eau de cologne (3-5%). Separated. In general, when trying to create a new fragrance product by mixing mixed spices, the top note that the fragrance immediately appears when sprayed, the middle note that expresses the characteristics of the fragrance well, and the last note that gives depth and volume to the fragrance must be in harmony with each other.

When a human inhales perfume, the fragrance molecules move along the nasal cavity and combine with olfactory receptors to generate an electrical signal. When the electrical signal generated at this time is transmitted to the cerebral cortex along the olfactory tract in the olfactory bulb, brain activation is achieved. Odor molecules contained in spices are known to affect the human brain, change brain waves, and induce physiological and psychological changes.

When the cerebral cortex is activated by a certain stimulus, brain waves are generated, which are electrical signals generated between neurons in the nervous system of the human cerebral cortex. The cerebrum occupies more than 75% of the total human brain volume and is divided into left and right hemispheres. The cerebral cortex, the surface of the cerebrum, has a wrinkled shape with a round protruding gyrus and a grooved sulcus.

Electrodes that can amplify cerebral electrical activity are attached to the scalp, the obtained waveforms are recorded and analyzed as a graph, and EEG, an electrical signal generated by the autonomous activity of the brain, records and observes brain activity to improve human brain performance. It is used as an indicator of human psychophysiological functions such as arousal, activity, anxiety and stability. EEG is used in cognitive science, medicine, pharmacy, and criminal investigation because it has the advantage of being able to record and measure objectively, non-invasively, and continuously.

EEG mainly shows a frequency of 0 to 60 Hz and an amplitude of 5 to 300 μV. Depending on the frequency range, delta waves (0 to 4 Hz), theta waves (4 to 8 Hz), alpha waves (8 to 13 Hz), beta waves It is divided into waves (13-30 Hz) and gamma waves (30-50 Hz). Delta waves and theta waves are slow waves and appear when thinking and working using information inside the brain. Beta waves higher than alpha waves are fast waves. In EEG analysis, relative power is used to reduce individual differences such as difference in thickness of the skull, electrical state of the scalp, and tension, as well as absolute power, and to analyze an accurate EEG response.

Korean Patent Publication No. 2015-0062967 discloses a perfume composition reproducing the scent of Korean fir tree, and Korean Patent Registration No. 2020384 discloses a cosmetic composition for skin whitening and wrinkle improvement containing essential oil derived from Korean fir tree. It is different from the present invention in specific active ingredients and effects.

The present invention has been devised by the above needs, and in the present invention, AHB (Absolute high beta power spectrum) To provide a fruity floral fragrance composition that increases the concentration of the human brain by inducing an increase in RMB (Relative mid beta power spectrum), RHB (Relative high beta power spectrum) and SEF50 (Spectral edge frequency 50%) There is a purpose.

In order to solve the above problems, the present invention provides Aldehyde C14, Aldehyde C16, Ambroxan, Benzyl acetate, Benzyl salicylate, Bergamot ) essential oil, cis-3-hexenol, dipropylene glycol, galaxolide, Guaiac wood essential oil, Hedion, heliotropin ( Heliotropine), Hydroxy citronellal%, Linalool, Linalyl acetate, Methyl anthranilate, Orange oil, Phenyl ethyl alcohol alcohol), Sandalwood essential oil, Tonalide, Verdox, and a perfume mixture containing extracts of Korean fir tree extract as active ingredients, increasing AHB (Absolute high beta power spectrum) in the left prefrontal cortex characterized by increasing Relative mid beta power spectrum (RMB) in the left temporal lobe, Relative high beta power spectrum (RHB) in the left prefrontal lobe, and increasing SEF50 (Spectral edge frequency 50%) in the left temporal lobe A flavoring composition for increasing the concentration of the human brain is provided.

In addition, the present invention provides a health functional food for increasing the concentration of the human brain containing the flavor composition.

In addition, the present invention provides a cosmetic composition for increasing the concentration of the human brain comprising the above fragrance composition.

In addition, the present invention is aldehyde C14 (Aldehyde C14), aldehyde C16 (Aldehyde C16), ambroxan, benzyl acetate, benzyl salicylate, bergamot essential oil, cis- cis-3 Hexenol, Dipropylene glycol, Galaxolide, Guaiac wood essential oil, Hedion, Heliotropine, Hydroxy Hydroxy citronellal%, Linalool, Linalyl acetate, Methyl anthranilate, Orange oil, Phenyl ethyl alcohol, Sandalwood It is prepared by mixing Sandalwood essential oil, Tonalide, Verdox, and Korean fig tree extract, and increases AHB (Absolute high beta power spectrum) in the left frontal lobe and RMB (Relative mid beta power) in the left temporal lobe. spectrum), RHB (Relative high beta power spectrum) in the left frontal lobe, and SEF50 (Spectral edge frequency 50%) in the left temporal lobe. Method for producing a spice mixture for increasing concentration in the human brain, characterized in that provides

In addition, the present invention provides a spice mixture for increasing the concentration of the human brain prepared by the above method.

The combination flavor of the present invention has AHB indicators (Absolute high beta power spectrum), RMB indicators (Relative mid beta power spectrum), RHB indicators (Relative high beta power spectrum) and SEF50 indicators (Spectral edge frequency 50%) in specific brain regions. Since it has the property of increasing the concentration of the brain by increasing the concentration, it can be used to develop various fragrance industrial products for consumers who want concentration.

1 is a photograph showing the 10-20 international standard electrode attachment method, which presents a site where electrodes are attached to the human brain for EEG measurement.
2 is a photograph showing a state in which a cap for electroencephalogram (EEG) is put on the brain of a subject and an EEG is measured.

In order to achieve the object of the present invention, the present invention provides Aldehyde C14, Aldehyde C16, Ambroxan, Benzyl acetate, Benzyl salicylate, Bergamot (Bergamot) essential oil, cis-3-Hexenol, Dipropylene glycol, Galaxolide, Guaiac wood essential oil, Hedion, Heliotrope Heliotropine, Hydroxy citronellal%, Linalool, Linalyl acetate, Methyl anthranilate, Orange oil, Phenyl ethyl alcohol ( Phenyl ethyl alcohol), Sandalwood essential oil, Tonalide, Verdox, and Absolute high beta power spectrum (AHB) in the left prefrontal cortex containing a mixture of fragrances mixed with extracts of Korean fig tree as active ingredients Characterized by increasing RMB (Relative mid beta power spectrum) in the left temporal lobe, increasing RHB (Relative high beta power spectrum) in the left prefrontal lobe, and increasing SEF50 (Spectral edge frequency 50%) in the left temporal lobe It provides a fragrance composition for increasing the concentration of the human brain.

In the perfume composition of the present invention, the perfume composition preferably contains 1.2 to 1.8% by weight of Aldehyde C14, 1.2 to 1.8% by weight of Aldehyde C16, 1.2 to 1.8% by weight of Ambroxan, Benzyl acetate 1.2-1.8% by weight, Benzyl salicylate 3.5-4.5% by weight, Bergamot essential oil 11-13% by weight, cis-3-Hexenol 3.5 ~4.5 wt%, Dipropylene glycol 6.5~7.5 wt%, Galaxolide 1.8~2.2 wt%, Guaiac wood essential oil 6.5~7.5 wt%, Hedion 9 -11 wt%, Heliotropine 1.8-2.2 wt%, Hydroxy citronellal 1.8-2.2 wt%, Linalool 9-11 wt%, Linalyl acetate 5.5 to 6.5% by weight, 3.5 to 4.5% by weight of methyl anthranilate, 2.7 to 3.3% by weight of orange oil, 5.5 to 6.5% by weight of phenyl ethyl alcohol, sandalwood ) 4.5 to 5.5% by weight of essential oil, 3.5 to 4.5% by weight of Tonalide, 3.5 to 4.5% by weight of Verdox and 1.8 to 2.2% by weight of Korean fir tree extract,

More preferably, 1.5% by weight of Aldehyde C14, 1.5% by weight of Aldehyde C16, 1.5% by weight of Ambroxan, 1.5% by weight of Benzyl acetate, Benzyl salicylate salicylate) 4% by weight, Bergamot essential oil 12% by weight, cis-3-Hexenol 4% by weight, dipropylene glycol 7% by weight, Galaxolide 2% by weight %, Guaiac wood essential oil 7% by weight, Hedion 10% by weight, Heliotropine 2% by weight, Hydroxy citronellal 2% by weight, Linalool ) 10% by weight, linalyl acetate 6% by weight, methyl anthranilate 4% by weight, orange oil 3% by weight, phenyl ethyl alcohol 6% by weight, It may consist of 5% by weight of essential oil of Sandalwood, 4% by weight of Tonalide, 4% by weight of Verdox and 2% by weight of Korean fig tree extract.

The AHB power spectrum is a high beta wave corresponding to 20 to 30 Hz, and appears predominantly during relatively complex reasoning or complex information processing with high mental load, or in a state of emotional tension. The RMB index is the ratio of the absolute power of the mid-beta band to the absolute power of the entire frequency domain in the EEG spectrum, and an increase in RMB means an increase in attentional concentration. The RHB index means the ratio of High Beta waves in the whole, and High Beta waves appear when performing relatively complex reasoning or complex information processing activities with high mental load. SEF50 means a specific frequency value in which the area up to a specific frequency value occupies 50% of the area in the frequency domain, and is used when evaluating the level of arousal.

The present invention also provides a health functional food for increasing the concentration of the human brain containing the flavor composition of the present invention.

The health functional food includes various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, coloring agents and enhancers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, It may contain pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, and the like. In addition, it may contain fruit flesh for the production of natural fruit juice and vegetable beverages. These components may be used independently or in combination.

The health functional food of the present invention may contain various flavoring agents or natural carbohydrates as additional ingredients. The natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as thaumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used.

The present invention also provides a cosmetic composition for increasing the concentration of the human brain comprising the fragrance composition of the present invention.

The cosmetic composition includes softening lotion, astringent lotion, nutrient lotion, nutrient lotion, nutrient essence, nutrient cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, gel, powder, and makeup base. , It is preferable to have a formulation selected from the group consisting of body lotion, body cream, body oil, body essence, lotion, ointment, gel, cream, patch and spray, but is not limited thereto.

The present invention also relates to Aldehyde C14, Aldehyde C16, Ambroxan, Benzyl acetate, Benzyl salicylate, Bergamot essential oil, cis- cis-3 Hexenol, Dipropylene glycol, Galaxolide, Guaiac wood essential oil, Hedion, Heliotropine, Hydroxy Hydroxy citronellal%, Linalool, Linalyl acetate, Methyl anthranilate, Orange oil, Phenyl ethyl alcohol, Sandalwood It is prepared by mixing Sandalwood essential oil, Tonalide, Verdox, and Korean fig tree extract, and increases AHB (Absolute high beta power spectrum) in the left frontal lobe and RMB (Relative mid beta power) in the left temporal lobe. spectrum), RHB (Relative high beta power spectrum) in the left frontal lobe, and SEF50 (Spectral edge frequency 50%) in the left temporal lobe. Method for producing a spice mixture for increasing concentration in the human brain, characterized in that provides

More specifically, the method for producing a spice mixture for increasing the concentration of the human brain of the present invention contains 1.2 to 1.8 wt% of aldehyde C14 and 1.2 to 1.8 wt% of aldehyde C16, based on the total weight of the spice mixture. , Ambroxan 1.2-1.8% by weight, Benzyl acetate 1.2-1.8% by weight, Benzyl salicylate 3.5-4.5% by weight, Bergamot essential oil 11-13% by weight, cis-3-hexenol 3.5 ~ 4.5% by weight, dipropylene glycol 6.5 ~ 7.5% by weight, galaxolide 1.8 ~ 2.2% by weight, Guaiac wood Essential oil 6.5~7.5 wt%, Hedion 9~11 wt%, Heliotropine 1.8~2.2 wt%, Hydroxy citronellal 1.8~2.2 wt%, Linalool 9 to 11% by weight, linalyl acetate 5.5 to 6.5% by weight, methyl anthranilate 3.5 to 4.5% by weight, orange oil 2.7 to 3.3% by weight, phenyl ethyl alcohol (Phenyl ethyl alcohol) 5.5-6.5% by weight, Sandalwood essential oil 4.5-5.5% by weight, Tonalide 3.5-4.5% by weight, Verdox 3.5-4.5% by weight and Korean fir tree extract 1.8-2.2% by weight It can be prepared by mixing %,

More specifically, based on the total weight of the fragrance mixture, 1.5% by weight of Aldehyde C14, 1.5% by weight of Aldehyde C16, 1.5% by weight of Ambroxan, and 1.5% by weight of Benzyl acetate. Benzyl salicylate 4% by weight, Bergamot essential oil 12% by weight, cis-3-Hexenol 4% by weight, Dipropylene glycol 7% by weight, gall Galaxolide 2 wt%, Guaiac wood essential oil 7 wt%, Hedion 10 wt%, Heliotropine 2 wt%, Hydroxy citronellal 2 % by weight, Linalool 10% by weight, Linalyl acetate 6% by weight, Methyl anthranilate 4% by weight, Orange oil 3% by weight, Phenyl ethyl alcohol (Phenyl ethyl alcohol) 6% by weight, Sandalwood essential oil 5% by weight, Tonalide 4% by weight, Verdox 4% by weight, and Korean fig tree extract 2% by weight.

The present invention also provides a spice mixture for increasing the concentration of the human brain prepared by the above method.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only to illustrate the present invention, and the content of the present invention is not limited to the following examples.

1. Sample acquisition and storage

In the case of ficus ficus essential oil used in the present invention, ficus ficus samples were collected from the ficus ficus colony in Namjeon 1-ri, Inje-gun, Gangwon-do. After being transferred to Kangwon National University natural product laboratory immediately after collection, essential oil extraction was carried out while storing at 4℃ in a low-temperature warehouse (2nd floor of Agricultural Building No. 3) at the Institute of Agriculture and Life Sciences, Kangwon National University.

The perfume composition of the present invention used to develop the Korean fig tree perfume contains Aldehyde C14, Aldehyde C16, Ambroxan, Benzyl acetate, Benzyl salicylate , Bergamot essential oil, cis-3-Hexenol, Galaxolide, Guaiac wood essential oil, Hedion, Heliotropine, Hyde Hydroxy citronellal, Linalool, Linalyl acetate, Methyl anthranilate, Orange oil, Phenyl ethyl alcohol, Sandalwood (Sandalwood) essential oil, Tonalide, Verdox, and Dipropylene glycol were purchased from domestic raw material suppliers in samples with Cas Numbers clearly specified.

2. Extraction of natural essential oil of Korean locust tree

Extraction of essential oil from fir tree samples was performed using a steam distillation apparatus (EssenLab-Plus, HANIL LABTECH, Korea). 5 L of distilled water was put in the steam generating part of the steam distillation device, 1 kg of fir tree sample was put in the essential oil generating part, and 2℃ cooling water was continuously flowed through the cooling pipe. Steam was generated while maintaining the temperature of the steam generating part at 100 ° C for 90 minutes.

When essential oil was produced for 15 minutes, 30 minutes, 45 minutes, 60 minutes, 75 minutes, and 90 minutes from the start of the experiment, the recovery rate was 013%, 017%, 016%, 012%, 010%, and 009%, respectively. The production of Korean fir tree essential oil was maximum at 30 minutes after the start of the experiment, and showed a tendency to decrease gradually thereafter.

After completion of the experiment, in order to collect only pure essential oil, the essential oil layer was passed through a triangular funnel containing anhydrous sodium sulfate to remove moisture and impurities, and the collected essential oil was stored in a refrigerator at 4 ° C.

Example 1. Flavor evaluation of Korean locust tree natural essential oil

The fragrance evaluation of the obtained locust tree essential oil was performed by 5 expert panelists from the Rose of Sharon, a perfume development club at Kangwon National University, who had been trained in the sense of smell for more than 6 months. After soaking the extracted natural essential oil solution (100%) on a scent paper (0.5 cm × 5 cm), place the scent paper at a position of 5 cm from the nose of the panel to smell the scent and evaluate the characteristics of the scent using the fragrance evaluation term ( It was described using Table 2). The fragrance types used in the sensory evaluation were aldehyde, animalic, balsamic, citrus, coniferous, earthy, floral, and fruity. , green, herbal, medicinal, minty, marine, mossy, musky, oily, oriental, powdery (powdery), smoky (smoky), spicy (spicy), sweet (sweet), woody (woody), and commonly used by the fragrance evaluation expert group.

Fragrance types and characteristics of essential oils dreadlocks type characteristic example Aldehyde Oil or fat-like aroma Aldehyde C9 Animalic incense with animal odor Civet cat oil Balsamic Sweet and warm feeling like normal vanilla Benzoin Citrus Refreshing citrus scent, sweet and fresh feeling Bergamot Coniferous Refreshing and clean pine scent Cajuput Earthy Unique soil scent in the air when it rains Vetiver Floral The scent of a single flower or a bouquet of flowers Jasmin, Rose Fruity Fragrance expressing peach, strawberry, tropical fruit, etc. Apple, Plum Green Fragrance felt when the leaves are cut or rubbed Galbanum Herbal A strong, pungent plant scent, such as the scent of mugwort Lavender Medicinal scent reminiscent of medicine Anis Minty Menthol flavor of peppermint or spearmint Peppermint Marine Slightly salty scent reminiscent of the cool sea Calone Wet Moist fragrance with an aqua-type scent Musky Musk-like incense Musk Oily Oily scent like olive oil Olive Oriental Mysterious incense that is widely supplied in the Orient Opopanax Powdery Spices that make you feel the powder Heliotropine Smokey Smoke-like smell from smoked fish, etc. Spicy The pungent scent of spices Clove Sweet Sweet, fragrant, pleasurable feeling Ethyl maltol Woody Subtle and profound woody scent from trees Sandalwood

The results of evaluating the fragrance of the essential oil extracted using a steam distillation are shown in Table 2 below.

Fragrance type of fir tree essential oil Fragrance Type Abies koreana Wilson. Aldehyde Animalic Balsamic Citrus ● Coniferous ● Earthy ● Floral Fruity Green ● Herbal ● Medicinal Minty ● Marine Wet ● Musky Oily ● Oriental Powdery Smokey ● Spicy ● Sweet Woody ●

Example 2. Development of perfume composition using natural essential oil of Korean locust tree and investigation of consumer preference

Essential oil extracted from the fir tree by steam distillation and aldehyde C14, aldehyde C16, ambroxan, benzyl acetate, benzyl salicylate, bergamot ) Essential Oil, cis-3 Hexenol, Galaxolide, Guaiac Wood Essential Oil, Hedion, Heliotropine, Hydroxy Citronellal Hydroxy citronellal, Linalool, Linalyl acetate, Methyl anthranilate, Orange oil, Phenyl ethyl alcohol, Sandalwood essential oil , Tonalide, Verdox, and dipropylene glycol were combined to develop four new flavor combinations (Table 3). Korean locust essential oil has its own unique fragrance and showed a very new fragrance when mixed with perfume compositions.

The fragrance composition of the present invention is a fruity floral-based combination fragrance, characterized in that it gives a cute and lovely image with a fragrance inspired by the colorful colors and fruit-like appearance of flowers and fruits of Korean fig tree.

Composition and composition ratio for the development of new flavors flavoring ingredients One 2 3 4 One Aldehyde C14 1.50 1.50 1.50 1.50 2 Aldehyde C16 1.50 1.50 1.50 1.50 3 Ambroxan 1.50 1.50 1.50 1.50 4 Benzyl acetate 1.50 1.50 1.50 1.50 5 Benzyl salycillate 4.00 4.00 4.00 4.00 6 Bergamot 11.00 11.00 12.00 12.00 7 cis-3-hexenol 4.00 4.00 4.00 4.00 8 Dipropylene glycol (DPG) 7.00 7.00 7.00 7.00 9 Galaxolide 2.00 2.00 2.00 2.00 10 Guaiac wood 7.00 7.00 7.00 7.00 11 Hedion 11.00 11.00 10.00 10.00 12 Heliotropine 1.50 2.00 2.00 1.50 13 Hydroxycitronellal 3.00 3.00 2.00 2.00 14 Linalool 12.00 12.00 10.00 10.00 15 Linalyl acetate 7.00 7.00 6.00 7.00 16 Methyl anthranilate 1.00 1.00 4.00 4.00 17 Orange oil 3.00 2.00 3.00 2.00 18 Phenyl ethyl alcohol (PEA) 4.00 3.00 6.00 7.00 19 Sandalwood 7.50 7.00 5.00 5.50 20 Tonalide 4.00 4.00 4.00 4.00 21 Verdox 3.00 5.00 4.00 3.00 22 Korean fir tree extract (5%) 2.00 2.00 2.00 2.00 Sum 100 100 100 100

The preference survey for the four flavor combinations was conducted at Kangwon National University College of Agriculture and Life Science Building No. 2, College of Agriculture and Life Science Building No. 3, Eco-friendly Center, and Mirae Plaza, and a total of 100 people were surveyed. After soaking the undiluted solution (100%) of each combination spice on a test paper (0.5 cm × 5 cm), place the test paper at a position of 5 cm from the nose of the panel, smell it, and score 10 points for the most preferred spice and 10 points for the most non-preferred spice. was given 1 point. An average value was obtained based on the preference results, and it is shown in Table 4.

Consumer Preference for 4 Types of Spices consumer preference Mixed Spices 1 Mixed Spices 2 Combination Spices 3 Combination Spices 4 medium 6.0 7.2 8.8 7.1

As a result of the preference survey on the four flavor compositions, consumers preferred the combination flavor composition 3 the most. Combination spice 1 had the lowest preference.

Example 3. Effect of Fruity Floral Fragrance Combination Fragrance 3 on Human EEG

Through Example 2, electroencephalogram (EEG) was measured to find out what effect it has on human brain activity when inhaling spice 3, which had the highest consumer preference. EEG measurements were performed on a total of 20 subjects, 10 males and 10 females, in a 32.4 m ² laboratory maintained at a temperature of 23° C. and 50% humidity in which noise was not mixed as much as possible.

The EEG measuring device used for EEG measurement was the Quick-30 Dry EEG Headset (Cognionics, Inc. USA), Cognionics' Cognionics Data Acquisition Software was used to measure the participant's EEG signal, and LAXTHA's data collection equipment and analysis were used for EEG data analysis. A program (Telescan) was used. The experiment participants were asked to wear an EEG cap with 29 ground electrodes distributed using the International 10~20 System, and the ground electrodes were attached to each EEG part using Flex Sensors (Cognionics, Inc. USA). Also, a reference electrode was attached to the left ear lobe using Drypad Sensors.

The air cylinder outlet was placed 5 cm in front of the participant, and measurements were taken for a total of 120 seconds (2 minutes), 60 seconds each before inhalation (non-stimulus) and inhalation (scent stimulation).

Data processing obtained through EEG measurement was performed by extracting only representative EEG signals for 50 seconds among the EEG signals of the experiment participants, performing batch processing, and then using SPSS (SPSS 24.0 version), a statistical analysis software program. A paired-sample T test was performed. In this Example, the types of EEG indicators for data analysis obtained before (non-stimulation) and during inhalation (scent stimulation) of fruity floral fragrance are shown in Table 5, and the meaning of each EEG indicator is shown in Table 6.

Types of EEG indicators used for data analysis No. Analysis indicator Full name of EEG analysis index Wavelength (Hz) One AT Absolute Theta Power Spectrum 4 to 8 2 AA Absolute Alpha Power Spectrum 8 to 13 3 AB Absolute Beta Power Spectrum 13 to 30 4 AG Absolute Gamma Power Spectrum 30 to 50 5 AFA Absolute Fast Alpha Power Spectrum 11~13 6 ASA Absolute Slow Alpha Power Spectrum 8-11 7 ALB Absolute Low Beta Power Spectrum 12 to 15 8 AMB Absolute Mid Beta Power Spectrum 15 to 20 9 AHB Absolute High Beta Power Spectrum 20 to 30 10 RT Relative Theta Power Spectrum (4~8)/(4~50) 11 RA Relative Alpha Power Spectrum (8~13)/(4~50) 12 RB Relative Beta Power Spectrum (13-30)/(4-50) 13 RG Relative Gamma Power Spectrum (30~50)/(4~50) 14 RFA Relative Fast Alpha Power Spectrum (11~13)/(4~50) 15 RSA Relative Slow Alpha Power Spectrum (8~11)/(4~50) 16 RLB Relative Low Beta Power Spectrum (12~15)/(4~50) 17 RMB Relative Mid Beta Power Spectrum (15~20)/(4~50) 18 RHB Relative High Beta Power Spectrum (20~30)/(4~50) 19 RST Ratio of SMR to Theta (12~15)/(4~8) 20 RMT Ratio of Mid Beta to Theta (15~20)/(4~8) 21 RSMT Ratio of (SMR-Mid Beta) to Theta (12~20)/(4~8) 22 RAHB Ratio of Alpha to High Beta (8~13)/(20~30) 23 SEF50 Spectral Edge Frequency 50% 4 to 50 24 SEF90 Spectral Edge Frequency 90% 4 to 50 25 ASEF Spectral Edge Frequency 50% of Alpha Spectrum Band 8 to 13

Meaning of EEG indices used in data analysis No. Analysis indicator meaning One AT Absolute power of Theta waves (meditation, when sleepy) 2 AA Alpha wave absolute power (relaxation, stability) 3 AB Absolute power of beta wave (tension, arousal, activation, cognitive function) 4 AG Absolute power of gamma waves (high level of cognitive function or anxiety or excitement) 5 AFA Absolute power of fast alpha waves (comfort and immersion) 6 ASA Absolute power of slow alpha wave (relaxation) 7 ALB Absolute power of low beta waves, also called SMR waves (attention or alertness) 8 AMB Absolute power of mid beta waves (concentration, attention) 9 AHB Absolute power of high beta waves (stress, mental load, tension) 10 RT Percentage of theta waves in the total 11 RA Percentage of Alpha waves in the total 12 RB The ratio of beta waves to the total 13 RG Ratio of gamma waves to the total 14 RFA Percentage of Fast Alpha waves in total 15 RSA Percentage of Slow Alpha waves in the total 16 RLB Percentage of Low Beta waves in the total 17 RMB Percentage of Mid Beta waves in the total 18 RHB Percentage of High Beta waves in the total 19 RST Ratio of Theta waves in SMR waves in total 20 RMT Ratio of Mid Beta to Theta waves in total 21 RSMT SMR~Mid Beta wave/Theta wave (brain wave concentration index) 22 RAHB Alpha wave/High Beta wave (stabilization and relaxation) 23 SEF50 Median Frequency brain activity (brain wave arousal) 24 SEF90 Median Frequency brain activity (mental load, stress) 25 ASEF SEF50 of Alpha wave (comfort: there are many Fast Alpha waves)

As a result of analyzing the effect of the most preferred combination flavor 3 in Example 2 of the present invention on human brain waves, only the AHB index, RMB index, RHB index, and SEF50 index among 25 indexes were statistically significant. ( p <0.05) Only this will be explained.

1. AHB (Absolute high beta power spectrum)

Inhalation of the combination flavor 3 increased the subjects' AHB index in the left prefrontal cortex (Fp1) (Table 7). The AHB power spectrum is a high beta wave corresponding to 20 to 30 Hz. It appears predominantly during relatively complex reasoning or complex information processing with high mental load, or in a state of emotional tension. Since the AHB power spectrum increased with the inhalation of flavor combination 3, it is understood that flavor combination 3 induces emotional tension.

Changes in AHB index due to inhalation of blended spice 3 Absolute high beta power spectrum (AHB) brain location inhalation state number of subjects average Standard Deviation t-test p left prefrontal lobe
(Fp1) Before 20 7.3533 1.1866 -2.1349 0.0460 During 20 8.3353 1.5441 right prefrontal lobe
(Fp2) Before 20 7.0201 1.0875 -1.4592 0.1608 During 20 7.6696 1.3570 Left frontal lobe (F3) Before 20 7.6595 1.2507 -1.9631 0.0644 During 20 8.7902 1.6542 Right frontal lobe (F4) Before 20 7.1146 1.1736 -1.5608 0.1351 During 20 8.2095 1.7558 Left temporal lobe (T3) Before 20 9.0324 1.7838 -0.8041 0.4313 During 20 10.6720 2.0192 right temporal lobe (T4) Before 20 7.1155 1.1213 -0.4906 0.6293 During 20 7.6877 1.3351 Left parietal lobe (P3) Before 20 7.7723 1.2700 -0.6177 0.5441 During 20 8.1194 1.3861 right parietal lobe (P4) Before 20 6.9309 1.1196 -1.3791 0.1839 During 20 7.4203 1.2919

2. Relative mid beta power spectrum (RMB)

Inhalation of the combination flavor 3 increased the subjects' RMB index in the left temporal lobe (T3) (Table 8). It refers to the spectrum value obtained by dividing the wave region (15 to 20 Hz) by the entire frequency region (4 to 50 Hz). The fact that the RMB index increased by inhalation of the blended spice 3 indicates that the level of alertness in the brain increased.

Changes in RMB index due to inhalation of blended spice 3 Relative mid beta power spectrum (RMB) brain location inhalation state number of subjects average Standard Deviation t-test p left prefrontal lobe
(Fp1) Before 20 0.0699 0.0059 0.0744 0.9415 During 20 0.0697 0.0056 right prefrontal lobe
(Fp2) Before 20 0.0706 0.0069 0.3982 0.6949 During 20 0.0689 0.0058 Left frontal lobe (F3) Before 20 0.0693 0.0067 -0.6089 0.5498 During 20 0.0714 0.0061 Right frontal lobe (F4) Before 20 0.0664 0.0069 -0.1659 0.8700 During 20 0.0670 0.0064 Left temporal lobe (T3) Before 20 0.0986 0.0085 -2.1859 0.0415 During 20 0.1082 0.0089 right temporal lobe (T4) Before 20 0.0933 0.0074 -0.0550 0.9567 During 20 0.0936 0.0090 Left parietal lobe (P3) Before 20 0.0835 0.0096 0.4529 0.6558 During 20 0.0808 0.0073 right parietal lobe (P4) Before 20 0.0808 0.0090 1.2408 0.2298 During 20 0.0745 0.0065

3. Relative high beta power spectrum (RHB)

Inhalation of the combination flavor 3 increased the subjects' RHB index in the left prefrontal cortex (Fp1) (Table 9). The RHB index is the ratio of the absolute power of the high beta band to the absolute power of the entire frequency domain in the brain wave spectrum, and means the spectrum value obtained by dividing the high beta wave region (20-30 Hz) by the entire frequency region (4-50 Hz). . The fact that RHB was increased by inhalation of the combination spice 3 indicates that the activity state of the brain was enhanced, that is, the level of tension was increased.

Changes in RHB index due to inhalation of blended spice 3 Relative high beta power spectrum (RHB) brain location inhalation state number of subjects average Standard Deviation t-test p Fp1 Before 20 0.1006 0.0095 -2.6807 0.0148 During 20 0.1151 0.0109 Fp2 Before 20 0.1034 0.0103 -1.4139 0.1736 During 20 0.1119 0.0114 F3 Before 20 0.0965 0.0113 -1.2880 0.2132 During 20 0.1037 0.0113 F4 Before 20 0.0891 0.0107 -1.1707 0.2562 During 20 0.0948 0.0117 T3 Before 20 0.1486 0.0154 -0.4920 0.6284 During 20 0.1544 0.0132 T4 Before 20 0.1447 0.0128 -0.1083 0.9149 During 20 0.1460 0.0143 P3 Before 20 0.0973 0.0082 -1.6612 0.1131 During 20 0.1060 0.0098 P4 Before 20 0.0947 0.0090 -1.9812 0.0622 During 20 0.1047 0.0107

4. SEF50 (Spectral edge frequency 50%)

Inhalation of the combination flavor 3 increased the subjects' SEF50 (spectral edge frequency 50%) index in the left temporal lobe (T3) (Table 10). The SEF50 index is a standard scale for examining the activity of the cerebral cortex for cognitive activities, and can identify workload or arousal status. It is a method of quantifying how much the brainwave power spectrum distribution is biased toward high frequencies compared to low frequencies by frequency, and is expressed as an area ratio for the entire frequency domain. . The fact that the SEF50 index was increased by inhalation of the mixed spice 3 indicates that the brain activity was enhanced.

Changes in SEF50 index due to inhalation of blended fragrance 3 SEF50 (Spectral edge frequency 50%) brain location inhalation state number of subjects average Standard Deviation t-test p left prefrontal lobe
(Fp1) Before 12 11.0781 0.3889 -1.4829 0.1545 During 12 11.6000 0.5861 right prefrontal lobe
(Fp2) Before 12 11.2469 0.4585 -0.6182 0.5438 During 12 11.4531 0.5698 Left frontal lobe (F3) Before 12 10.6750 0.2788 -1.8110 0.0860 During 12 11.3688 0.5278 Right frontal lobe (F4) Before 12 10.7625 0.5484 -1.1021 0.2842 During 12 11.1344 0.5435 Left temporal lobe (T3) Before 12 12.6031 0.7979 -2.2285 0.0381 During 12 15.0438 1.2374 right temporal lobe (T4) Before 12 13.0531 0.7921 -0.6682 0.5120 During 12 13.7156 1.1430 Left parietal lobe (P3) Before 12 11.0188 0.3052 -1.2940 0.2112 During 12 11.7313 0.6670 right parietal lobe (P4) Before 12 10.9500 0.3284 -1.3208 0.2023 During 12 11.3969 0.5549

Claims (3)

Aldehyde C14 1.2-1.8 wt%, Aldehyde C16 1.2-1.8 wt%, Ambroxan 1.2-1.8 wt%, Benzyl acetate 1.2-1.8 wt%, benzyl salicylic acid Benzyl salicylate 3.5~4.5% by weight, Bergamot essential oil 11~13% by weight, cis-3-Hexenol 3.5~4.5% by weight, Dipropylene glycol 6.5~ 7.5 wt%, Galaxolide 1.8-2.2 wt%, Guaiac wood essential oil 6.5-7.5 wt%, Hedion 9-11 wt%, Heliotropine 1.8-2.2 % by weight, Hydroxy citronellal 1.8-2.2% by weight, Linalool 9-11% by weight, Linalyl acetate 5.5-6.5% by weight, Methyl anthranilate 3.5-4.5 wt%, Orange oil 2.7-3.3 wt%, Phenyl ethyl alcohol 5.5-6.5 wt%, Sandalwood essential oil 4.5-5.5 wt%, Tonalide 3.5 ~4.5% by weight, 3.5~4.5% by weight of Verdox, and 1.8~2.2% by weight of Korean fig tree extract, containing a mixture of spices as active ingredients, increasing AHB (Absolute high beta power spectrum) in the left prefrontal cortex , When RMB (Relative mid beta power spectrum) is increased in the left temporal lobe, RHB (Relative high beta power spectrum) is increased in the left prefrontal lobe, and SEF50 (Spectral edge frequency 50%) is increased in the left temporal lobe A fragrance composition for increasing the concentration of the human brain, characterized in that it increases. Based on the total weight of the fragrance mixture, 1.2 to 1.8 wt% of Aldehyde C14, 1.2 to 1.8 wt% of Aldehyde C16, 1.2 to 1.8 wt% of Ambroxan, 1.2 to 1.8 wt% of Benzyl acetate ~1.8 wt%, Benzyl salicylate 3.5-4.5 wt%, Bergamot essential oil 11-13 wt%, cis-3-Hexenol 3.5-4.5 wt%, dipropylene Dipropylene glycol 6.5-7.5% by weight, Galaxolide 1.8-2.2% by weight, Guaiac wood essential oil 6.5-7.5% by weight, Hedion 9-11% by weight, Heliotrope Heliotropine 1.8-2.2% by weight, Hydroxy citronellal 1.8-2.2% by weight, Linalool 9-11% by weight, Linalyl acetate 5.5-6.5% by weight, methyl Methyl anthranilate 3.5-4.5% by weight, Orange oil 2.7-3.3% by weight, Phenyl ethyl alcohol 5.5-6.5% by weight, Sandalwood essential oil 4.5-5.5% by weight , Tonalide 3.5 ~ 4.5% by weight, Verdox 3.5 ~ 4.5% by weight and Korean fir tree extract 1.8 ~ 2.2% by weight are mixed to increase AHB (Absolute high beta power spectrum) in the left prefrontal cortex Relative mid beta power spectrum (RMB) was increased in the left temporal lobe, Relative high beta power spectrum (RHB) was increased in the left prefrontal lobe, and SEF50 (Spectral edge frequency 50%) was increased in the left temporal lobe. Method for producing a spice mixture for increasing the concentration of the human brain, characterized in that it increases. A spice mixture for increasing the concentration of the human brain prepared by the method of claim 2.

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