KR101843977B1 - Essential Oil Compound from Chamaecyparis obtusa and Cupressaceae and Abies koreana WILS to increase RSMT (ratio of (SMR~Mid beta) to theta), RB (relative beta power spectrum), RMT (ratio of mid beta to theta), AFA(absolute fast alpha power spectrum) - Google Patents

Abstract

More particularly, the present invention relates to an essential oil composition extracted from a mixture of white clover, sapwood, and coniferous tree. More specifically, a sample obtained by mixing a 1: 1: 1 weight ratio of a perilla leaf, (RSMT), the relative beta power spectrum (RB), and the ratio of mid beta to theta (RB) in the left prefrontal region (Fp1) of the brain, (AFA) in the left temporal lobe (T3) of the brain, which increases the concentration effect of the brain. Therefore, it is widely used in the fields of aromatherapy and aroma colloid requiring mental concentration Can be applied.

Description

{Essential Oil Compound from Chamaecyparis obtusa and Cupressaceae and Abies koreana WILS to increase RSMT (ratio of (SMR ~ Mid beta) to the theta) which increases EEG index RSMT, RB, RMT and AFA ), RB (relative beta power spectrum), RMT (ratio of mid beta to theta), AFA (absolute fast alpha power spectrum)

The present invention relates to an essential oil composition extracted from a mixed sample of a white clover, a deciduous tree, and a coniferous tree. More specifically, the present invention relates to an essential oil composition extracted from a mixture of a white liana, When the essential oil extracted by the distillation method was inhaled, RSMT (ratio of SMR to Mid beta) to theta, RB (relative beta power spectrum), RMT (ratio of mid beta to theta) and absolute fast alpha power spectrum ) To increase the mental concentration of the functional concentrate composition.

In the nerve cells that make up the human cerebral cortex, a minute electrical activity is taking place. The electroencephalogram is called electroencephalogram (EEG) that measures the potential fluctuations on the scalp through the tissue covering the surface of the cerebral cortex, the skull, and the scalp. To measure brain waves, electrodes are placed on the scalp, and electrical signals generated in the brain are input to the brain waves to record time-dependent electrical potentials. The resulting result is called an electroencephalogram (EEG). 2011. Clinical Physiology EEG. EEG is able to record temporal and spatial changes of brain activity that varies instantaneously. It has the advantage of measuring brain function noninvasively, continuously and continuously. It can record brain activity record, disease diagnosis, sleep study, psychotherapy It has been widely used and used in the fields of cognitive science, medicine, and pharmacy (Yoon Jung Soo, 2010. Clinical EEG, Koryo Medicine, p.1-77).

Human brainwaves have amplitudes of 5 ~ 300 μV and frequency ranges between 0 and 60 Hz. Depending on the frequency range, delta waves (0.5-3 Hz), theta waves (4-7 Hz), alpha waves (8-13 Hz) , beta waves (14 ~ 30Hz) and gamma waves (30Hz and above). The delta wave and theta wave with a frequency lower than that of the alpha wave with respect to the alpha wave are called a slow wave. The beta wave and the gamma wave with higher frequency than the alpha wave are called the fast wave. Usually the slow waves appear when the amplitude is high and when sleep or brain activity is low, and the fast wave is mainly when the amplitude is low and brain activity is promoted.

The frequency of the delta wave is 0.5 to 4 Hz and the amplitude is 100 to 200 μV, which is the largest and irregular in EEG. Regardless of age, it appears during sleep and does not appear during awakening.

The frequency of theta wave is 4 ~ 8 Hz and the amplitude is 10 ~ 50 μV. It is known to occur mostly in the occipital region and temporal region. It is an emotional stable state or an EEG mainly appearing in the process leading to sleep.

alpha is a fundamental wave with a frequency of 8 ~ 13 Hz and an amplitude of 10 ~ 150 μV. It is well recorded in the parietal lobe, especially in the occipital lobe, and is seen in arousal, stable and closed state of normal adult. alpha waves are classified into slow alpha waves (7 to 8 Hz), mid alpha waves (9 to 11 Hz) and fast alpha waves (12 to 13 Hz) depending on the frequency range. Kangwon National University).

The beta wave is a fast wave of 14-30 Hz and has a low amplitude of 5-10 μV. It appears when doing general work or conscious actions and is activated when there is mental activity such as information processing. It is classified into slow beta waves (12 ~ 15Hz), mid beta waves (15 ~ 20Hz) and high beta waves (20 ~ 30Hz) depending on the frequency range.

The gamma wave corresponds to the frequency range of 30 to 50 Hz and oscillates most rapidly among EEG waves. Emotional nervousness or tension, and active high mental functions such as reasoning and judgment.

The volatile organic compounds contained in the essential oil reach the olfactory epithelium through the nasal passages when the plant essential oil is inhaled, and the olfactory nerve cells in the olfactory epithelium sense this and transmit electrical signals to the brain (Richard Axel 1995. The molecular logic of smell. Scientific American. October 154-158). The signal from the olfactory neuron is interpreted in the olfactory bulb and is recognized in the brain via two olfactory signal pathways, one through the amygdala and the lateral hypothalamus without passing through the thalamus, (cerebral cortex), and the other is a pathway through the olfactory cortex (pyriform cortex) through the thalamus to the cerebral cortex (Zetorre et al. 1992 Localization and lateralization of human olfactory cortex. Letters to Nature 360: 339-340).

It has been known that electrical signals are transmitted along the olfactory signal pathway through the oil inhalation (Reed, 1992, Signaling pathways in odorant detection, Neuron 8: 205-209). Lavender essential oil increased theta wave in the frontal lobe, alpha wave in the frontal lobe and temporal lobe, and beta wave in the temporal lobe (Jung Ki Young, Kim Jae Moon, Lee Il Keun Kim Ji Hyun, Ju Eun Yeong, Hong Seung Bong, Hong Sik Kim, Cerebral Cortical Activity: A nonparametric statistical map using Low Rsolution Electromagnetic Tomography (LORETA) 22 (4): 334-339). Rose essential oils increase the alpha, delta, and theta waves to increase body fatigue (1985), "The Effects of Massage on the Relaxation and EEG in Middle-aged Women," Journal of Clinical Psychology, Vol. Citrus aurantiifolia essential oil induces an increase in low beta wave in the right temporal lobe (T4) and a mid beta-wave increase in the right frontal lobe (P4) and left temporal lobe (T3) (Lee, EEG on the EEG. Heracleum moellendorffii essential oil has been known to increase the high beta wave to induce mental concentration and arousal effect (Seong Moon Kim, Eun-Eun Lee, Kwangwam 2011. Flavor composition containing aquatic natural essential oil extracted from aqua leaves. Patent 10-2011-0112914).

(+) - limonene is known to increase the relative high beta wave in the right temporal lobe (T4) region, thereby inducing brain tension and concentrating the mind. However, terpinolene, the structural isomer of (+) - limonene, has been shown to increase the relative mid beta and relative fast alpha waves in the right prefrontal cortex (Fp2) to reduce brain tensions (Kandhasamy Sowndhararajan, Haeme Cho, Byungsun Yu, and Songmun Kim. 2015. Effect of olfactory stimulation of isomeric aroma compounds, (+) - limonene and terpinolene on human electroencephalographic activity. European Journal of Integrative Medicine 7: 561-566).

Chamaecyparis obtusa (Chamaecyparis obtusa) is an evergreen arboreous tree in Cupressaceae, 40 m in height, 2 m in diameter, and branches spread horizontally to form a conical water pipe. Leaves are ovate rhomboid, dull, thick in vagina, with one gland on the green surface, with white spots on the back side, and the leaves on the side are endless as ovate oval. The cones are round and have a diameter of 10 ~ 12 mm and are brown and consist of 8 pieces, and the seeds are two in each piece (Lee, Changbok, 2003.).

Thuja orientalis L. is an evergreen arboreous tree in the cupressaceae family, 25 m high and 1 m in diameter. Water pipes are irregularly distributed, grayish brown in color, split vertically, big branch is reddish brown, twig is green and develops in vertical direction. The leaves are scaly, pointed, oblong in the center, oval in the side, or wide lanceolate, with a few white spots. The ovary is ovate, 15 ~ 20mm long, 8 chambers are crossed, and seeds are 2 ~ 3 in one piece, 2 ~ 6 in one fruit, oval or ovate, 5 mm in length and blackish brown. The flowers bloom in April, and the fruit ripens in September (Lee Chang-bok, 2003.).

Abies koreana WILS. Is an evergreen tree in the pinaceae fir and has a height of 18 m. The cones are cylindrical, 4-6 cm in length, 20-28 mm in diameter, greenish brown or dark brown, seeds 6 mm long and wings 4.5 mm wide. According to the color of the cones, the blue conception, for. Chlorocarpa T.LEE, black conception, for. Nigrocarpa HATUS., Red conception, for. Rubrocarpa T. LEE, etc. (Lee, Chang-Bok, 2003. The original plant illustrations of Korean plants.

In addition, the following are described in the patent documents related to the extracts of Cryptomeria japonica, Cryptomeria japonica and Cryptomeria japonica.

Korean Patent Laid-Open Publication No. 10-2015-0057334 (a stress-relieving and psychological stability enhancing fragrance composition containing a conifer extract) contains a coniferous tree extract to reduce a high beta wave generated at the time of stress, But the effect of the essential oil composition extracted from a mixture of the white birch tree, the spruce tree and the conifer tree of the present invention to increase the RSMT, RB, and RMT indices of EEG indicators, thereby obtaining a mental concentration effect, is described There is no bar.

Korean Patent Laid-Open Publication No. 10-2015-0062328 (a cosmetic composition for preventing stressful hair loss containing essential oils) contains at least one selected from the group consisting of Gadamon oil and Western bladder oil, , No side effects during long-term use, suppression of scalp inflammation caused by mental stress, and protection of hair cells from stress. However, the present invention is not limited to the use of essential oils There is no description of the effect that the composition increases the RSMT, RB, and RMT indices, which are EEG indicators, to obtain a mental concentration effect.

Korean Patent Laid-Open Publication No. 10-2011-0092290 (plant extract composition affecting sleep) includes a therapeutically effective amount of a prolapse extract and a pharmacologically acceptable carrier, Extracts and jujube extracts or a mixture thereof. However, the essential oil composition extracted from a mixture of the white waxy tree, the celestial tree, and the spherical tree according to the present invention contains RSMT, RB, The effect of increasing the RMT index to obtain a mental concentration effect has not been described.

(Patent Document 0004) Korean Patent Laid-open No. 10-2015-0083033 (a composition for improving, preventing or treating eye diseases containing an extract of a cotton bud extract as an active ingredient) includes a composition for improving, preventing or treating an eye disease, However, the essential oil composition extracted from a mixture of the white waxy tree, the spruce tree and the conifer tree of the present invention increases the RSMT, RB, and RMT indexes, which are EEG indices, Is not described.

(Patent Document 0005) Korean Patent Laid-Open Publication No. 10-2015-008357 (Phytoncide Bathing Agent Extracted from Perennial Leaf Leaf and Method for Producing the Same) is a method of mixing phytoncide leaves, pine wood leaves, pine leaves, mugwort, However, the essential oil composition extracted from a mixture of the white wood, the spruce tree, and the wood of the present invention increases the RSMT, RB, and RMT indicators, which are EEG indices, to obtain a mental concentration effect There is no description.

(Patent Document 0006) Korean Patent Laid-Open No. 10-2015-0122495 (a method of manufacturing interior wood finishing materials and finishing materials of furniture board to which a crude wood extract raw material and an oil mixture are added) However, the essential oil composition extracted from a mixture of the white waxy wood, the spruce tree, and the sphincter of the present invention increases the RSMT, RB, and RMT indexes of the EEG, thereby increasing the mental concentration effect Is not described.

(Patent Document 0007) Korean Patent Laid-Open Publication No. 10-2011-0092290 (a plant extract composition which influences sleeping) discloses that the extract of Quercus variabilis is useful for the therapeutic treatment of insomnia or insomnia. However, , There is no description of the effect of the essential oil composition extracted from the spherical tree mixed sample to increase the RSMT, RB, and RMT indicators, which are EEG indices, to obtain a mental concentration effect.

(Patent Document 0008) Korean Patent Laid-Open Publication No. 10-2015-0062328 (a cosmetic composition for controlling stress-relieving hair loss containing essential oils) relates to a cosmetic composition containing a Western hibiscus oil which can prevent hair loss caused by stress . However, the effect of the essential oil composition extracted from a mixture of the white waxy tree, the spruce tree and the coniferous tree of the present invention to increase the RSMT, RB, and RMT indexes, which are EEG indicators, has not been described.

Korean Patent Publication No. 10-2015-0057334, (May 27, 2015) Korean Patent Publication No. 10-2015-0062328, (June 5, 2015) Korean Patent Publication No. 10-2011-0092290, (2011.08.17) Korean Patent Laid-Open No. 10-2015-0083033, (2015.07.16) Korean Patent Publication No. 10-2015-0089357, (2015.08.05) Korean Patent Publication No. 10-2015-0122495, (Nov. Korean Patent Publication No. 10-2011-0092290, (2011.08.17) Korean Patent Publication No. 10-2015-0062328, (June 5, 2015)

Jin Bok Hee. 2011. Clinical physiology EEG. Koryo medicine. p.1, Yoon Jung Soo. 2010. Clinical Electroencephalography. Koryo medicine. p.1-77, Moving. 2011. Effects of Lime and Clove on EEG. Kangwon National University Master Thesis, Richard Axel. 1995. The molecular logic of smell. Scientific American. October 154-158, Zetorre et al. 1992. Functional localization and lateralization of human olfactory cortex. Letters to Nature 360: 339-340, Reed. 1992. Signaling pathways in odorant detection. Neuron 8: 205-209, Jung Kyoung Jung, Jae Moon Kim, Il Keun Lee, Ji Hyun Kim, Eun Yoon Ju, Hong Seung Bong, Hong Sik Kim, 2004. Cerebral Cortical Activity by Directional Olfactory Stimulation: A Nonparametric Statistical Map Using Low Rsolution Electromagnetic Tomography (LORETA). Korean Journal of Neurology 22 (4): 334-339, Choi, Seung - Ki. 2006. Effects of abdominal massage using rose oil on relaxation and EEG in middle-aged women. Graduate School, Kyonggi University. p.51, Moving. 2012. Effects of Lime and Clove on EEG. Kangwon National University Master Thesis, Kim Sung - Moon, Director, Yong - Nam. 2011. A perfume composition comprising an aquatic natural essential oil extracted from an aquatic leaf. Korean Patent No. 10-2011-0112914, Kandhasamy Sowndhararajan, Haeme Cho, Byungsun Yu, and Songmun Kim. 2015. Effect of olfactory stimulation of isomeric aroma compounds, (+) - limonene and terpinolene on human electroencephalographic activity. European Journal of Integrative Medicine 7: 561-566, Lee Chang - bok. 2003. Plant Plants of Primary Color. Commercial area. P.147, Lee Chang - bok. 2003. Plant Plants of Primary Color. Commercial area. P.146, Lee Chang - bok. 2003. Plant Plants of Primary Color. Commercial area. P.131,

Therefore, the present invention is based on the fact that when the plant essential oil is inhaled, the volatile organic compounds contained in the essential oil pass through the nasal passage to reach the olfactory epithelium, and the olfactory nerve cells in the olfactory epithelium sense and transmit electrical signals to the brain The purpose of the present invention is to develop a mental concentration functional composition by exploring whether or not essential oils extracted from a mixture of white, white, and coniferous woods have a function of enhancing concentration by activating specific areas of the brain.

Also, the present invention relates to an essential oil composition extracted from a mixture of white, white, and coniferous wood using a steam distillation method. More specifically, the white tea tree leaves, (Ratio of (SMR to Mid beta) to theta), RB (relative beta power spectrum) and RMT (ratio of mid (RB)) were measured by inhalation of the essential oil extracted by steam distillation beta to theta, and absolute fast alpha power spectrum (AFA), thereby providing a composition having a mental concentration effect.

In order to accomplish the above object, the present invention utilizes citrus, coniferous, green, herbal, minty, spicy, which are very unique in scent, . Nevertheless, studies on them have been rarely carried out. As explained in the present invention, the essential oils extracted from cottonwood, saplings, and coniferous trees are used as an aromatherapy or essential oil for healing human body Can be widely used in the field of aroma therapy that heals by using psychology.

The present invention provides a psycho-concentrating functional composition comprising natural essential oils (hereinafter referred to as "Welsh onion tree, spruce tree, and sphincter essential oil) extracted from the leaves of Opuntia ficus-indica, Opuntia ficus-indica, and coniferous trees as an active ingredient. The essential oil has a ratio of (SMR to Mid beta) to theta, an RB (relative beta power spectrum) index, and a ratio of mid beta (RBT) to the prefrontal (Fp1) theta) index, and the absolute fast alpha power spectrum (AFA) index at the left temporal lobe (T3) region.

The relationship between the active ingredient and the content of each component of the essential oil is characterized by being D-Limonene> Bornyl acetate> alpha-Pinene> beta-Pinene> 3-Carene> Camphene> Terpinyl acetate> beta-Phellandrene> alpha-Elemol .

The present invention also relates to a method for producing natural essential oils (hereinafter referred to as " perilla seedlings ", " perilla seedlings ", " perilla seedlings ") extracted by steam distillation using the same weight ratio (1: 1: The present invention provides a psycho-concentrating functional composition, which comprises as an active ingredient. It is preferable that the above-mentioned tree leaves are mixed in the same weight ratio, and the error of each mixing ratio is preferably within 5% by weight of each tree leaf. That is, when one tree leaf is 5% by weight on the basis of other tree leaves, for example, one leaf is 100 grams, it is preferable that the other leaf is included in the range of 95 - 105 grams.

Essential oil is a term collectively referred to as volatile organic compounds extracted from leaves, stems, fruits, roots, and flowers of plants by various methods. In particular, the present invention relates to a method of extracting essential oils from leaves, Natural essential oil as an active ingredient. At this time, the present invention is characterized by extracting the essential oil from the leaves of P. monocotyledonous, C. saprophyticia, and P.

The white planthopper, safflower, and sphincter essential oil of the present invention can be extracted by various known extraction methods such as steam distillation method, organic solvent method, supercritical extraction method, and the steam distillation method is preferably used. In addition, it is more advantageous to extract the essential oil from the sample with the weight ratio of 1: 1: 1 in the ratio of 1: 1: 1, rather than extracting and mixing essential oil separately from each tree. , The recovery rate of the extracted essential oil was 0.87 ± 0.17%.

In the present invention, the following compounds are included in the white clover, spruce tree, and sphincter essential oil mixture. Alpha-Cubebene, alpha-Curcumene, Bicyclo [9.3.1] pentadeca-3,7-dien-12-ol, 4- Beta-Farnesene, alpha-Eudesmol, beta-Farnesene, beta-Cubebene, alpha-p-Dimethylstyrene, alpha-Eudesmol, beta-Farnesene, Beta-Phellandrene, alpha-Pinene, beta-Pinene, Rimuene, Santene, Linalool, Linalyl formate, Menth-2-en-1-ol, Fenchyl acetate, Geranyl acetate, alpha-Himachalene, Hinesol, Isoledene, D-Limonene, , Terpinen-4-ol, alpha-terpineol, gamma-terpinene, terpinyl acetate, alpha-Thujene, Thujopsene, Tricyclene.

The fragrance type of the present invention is composed of citrus, coniferous, earthy, green, herbal, minty, wet, oily, smoky, spicy and woody.

(SMR to Mid beta) to theta) and RB (relative beta power spectrum) in the left prefrontal cortex (Fp1) region of the EEG index as compared with before the inhalation ) And the absolute fast alpha power spectrum (AFA) index in the left temporal lobe (T3) area, and the mental concentration effect is increased. Hwasung tree, Suseong tree essential oil can be applied to aromatherapy to heal human body and aroma colloid healing human psychology and spirit.

The present invention can also be used in the fragrance industry, such as toiletries (perfumes, etc.), soap cleansing products (such as bath soaps), indoor cleaning products (such as glass Can be used for the production of industrial goods (synthetic resin, etc.), aroma goods (stationary products, etc.), environmental goods (office aromatic products, etc.) . In addition, if the wood is dipped in an untreated tree, a deciduous tree, or a coniferous tree essential oil for a certain period of time, the fragrance will be emitted into the air for a long time. This feature can also be applied to healing wood products.

The formulation of the white linen, spruce, and sphincter essential oils of the present invention is not particularly limited and may be any one selected from powders, granules, strays, and liquid phases.

On the other hand, the perilla seed oil, the perennial plant tree, and the conifer tree essential oil can be used by those skilled in the art having ordinary skill in the art, so detailed description will be omitted hereafter.

The ratio of (SMR to Mid beta) to theta) index, the relative beta power spectrum (RB), and the relative beta power spectrum (RBP) of the left prefrontal cortex (Fp1) region of the human brain when inhaled into the nasal cavity, The absolute fast alpha power spectrum (AFA) index was increased in the index, RMT (ratio of mid-beta to theta) index, and left temporal lobe (T3)

Therefore, the timber which has been dipped in dried wood for a certain period for a certain period of time has a mental concentration effect, so it can be applied to product development that focuses on the spirit of human beings. .

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a chromatogram obtained by gas chromatography analysis of an organic compound contained in an unshiu, a deciduous tree, and a sphincter essential oil.
2 is a photograph showing an international standard electrode attaching method.
FIG. 3 is a brain map showing changes in RSMT (ratio of (SMR to Mid beta) to theta) before inhalation and inhalation of unshiu wood, deciduous tree, and sphincter essential oil mixture.
FIG. 4 is a brain map showing changes in RB (relative beta power spectrum) before inhalation and inhalation of untreated white, opium, and coniferous essential oil mixtures.
FIG. 5 is a brain map showing changes in the ratio of mid-to-theta (RMT) before inhalation and inhalation of untreated wood, cinnabar, and coniferous essential oil mixtures.
FIG. 6 is a brain map showing absolute fast alpha (AFA) changes before inhalation and inhalation of albino wood, opium tree, and sphincter essential oil mixture.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

EXAMPLES Example 1. Extraction of natural white essential oil

The samples of the white, white, and saponified leaves used in the present invention were received from Professor Han Sang-Sup of Chonbuk National University. The samples were then shredded for 7 days, dried in a 40 ^° C dryer, dried for 96 hours, and then pulverized to a size of about 5 cm. 1 kg each of crushed white, white, and coniferous leaf samples were quantitatively mixed and then extracted with a steam distillation device. 2 L of distilled water was added to the water vapor generating portion of the steam distillation apparatus, 1 kg of the sample was placed in the oil generating portion, and 4 ^o C cooling water was continuously flowed in the cooling tube. The water vapor was generated by maintaining the temperature of the steam generation site at 100 ^° C for 2 hours. The essential oil contained in the peridotite tree, the peridotite tree, and the peridotium tree was condensed in the cooling tube and collected in the distillation water layer and the refinery layer in the receiver. To collect pure essential oil, the essential oil layer was passed through a triangular funnel containing anhydrous sodium sulfate. The collected essential oil was stored in a refrigerator at 4 ^° C until flavor analysis, component analysis, and EEG analysis.

The recovery rate of the essential oil extracted from the elixir of Quercus mongolica, Cryptomeria japonica and Pinus densiflora was 0.87% and the standard deviation was 0.17%. Table 1 below shows the recovery rates of the essential oils extracted from the blended samples of unshiu wood, spruce tree and conifer tree.

sample Recovery rate (%) Average
(%) Standard Deviation
(%) Repeat 1 Repeat 2 Repeat 3 Safflower, Cryptomeria, Spherical tree leaf samples 0.58 0.86 1.16 0.87 0.17

Example 2: Evaluation of fragrance of natural essential oils such as waxy wood,

The fragrance evaluation of the untwisted wood, the bamboo plant, and the coniferous tree essential oil obtained in Example 1 was carried out by an expert of Gangwon Perfumed Alchemy Co., Ltd. The natural essential oil extract (100%) extracted from the above Example 1 was wetted on a pouch (0.5 cm x 5 cm), and the characteristic of the fragrance was evaluated at 5 cm from the nose using the fragrance evaluation term. Respectively. The flavor type used in Gangwon Perfume Alchemy Co., Ltd. is aldehyde, animalic, balsamic, citrus, coniferous, earthy, floral, It has been found that fruity, green, herbal, medicinal, minty, marine, mossy, musky, oily, oriental oriental, powdery, smoky, spicy, sweet, and woody, which were typically used by a group of flavor assessment experts. In the present Example 2, the fragrance intensity of the unshiu wood, the spruce tree, and the sphincter of the essential oil was evaluated by setting the strongest fragrance to 10 and the fragrance which had no fragrance at zero.

(5), coriander (5), hazel (3), green (6), herbal (4), minty (2), smoky (6) ), Spicy (1) and Woody (6). Table 2 below shows the results of evaluating the fragrance of essential oils extracted from unshiu, wood, and coniferous trees.

Drape type Fragrance strength Aldehyde 0 Animalic 0 Balsamic 0 Citrus 5 Coniferous 5 Earthy 3 Floral 0 Fruity 0 Green 6 Herbal 4 Medicinal 0 Minty 2 Marine 0 Mossy 0 Musky 0 Oily 2 Oriental 0 Powdery 0 Smoky 6 Spicy One Sweet 0 Woody 6

Example 3 Analysis of Organic Compounds Contained in Evergreen Trees, Semitic Trees, and Spherical Trees

The components of the organic compounds contained in the essential oils extracted from the white birch woods, spruce trees and sphincter samples obtained in Example 1 were analyzed by a gas chromatography-mass spectrometer (GC-MS). Ten microliters of the elixir of Quercus mongolica, Cryptomeria japonica, and Coniferous tree essential oil were injected into the injection port of the Varian 1200 using a syringe. The inlet temperature was 250 ^° C and the injection mode was split (split ration 10: 1). The operating conditions of the gas chromatography (GC) are shown in Table 3 below. Columns were VF-5MS (30 x 0.25 mm, 0.25 μm) and the oven temperature was 50 ^° C (5 min) 5 ^o C / min - 250 ^o C (1 min), and 1 mL of helium was flown per minute. The operating conditions of the mass spectrometer (Varian 1200) were an ionization energy of 70 eV and a carrier gas flow of 1 mL per minute into helium. GC-MS was used for mass spectrometry data of NIST library. Table 3 below shows the analytical conditions of GC-MS used for the analysis of organic compounds contained in cottonwood, saplings and safflower essential oils.

Gas Chromatography Instrument Varian 1200 Injector 온도 250 ^o C (1 mL / min) Column VF-5MS (30 × 0.25 mm, 0.25 μm) Gas flow Helium (1 mL / min) 오빈 온도 50 ^o C (5 min) - 5 ^o C / min 250 ^o C (1 min) Detector 온도 250 ^o C Mass
Spectrometer Instrument Varian 1200 Ionization voltage EI, 70 eV

The components of the organic compounds contained in the essential oils extracted from the white clover, saphenous tree and coniferous wood samples obtained in Example 1 were analyzed by gas chromatography-mass spectrometry. As a result, 97.68% (Fig. 1) and the remaining 2.32% were unknown organic compounds (Table 4).

3-Carene (6.70%)> beta-Pinene (6.70%)> alpha-Pinene (9.55%)> Bornyl acetate (11.63%)> D-Limonene 6.69%), Camphene (6.39%), Terpinyl acetate (6.18%), beta-Phellandrene (3.83%) and alpha-Elemol (2.56%). Table 4 below shows the volatile organic compounds contained in cottonwood, saplings, and sunflower essential oils.

No. Compound RT CAS No. Area (%) One Santene 7.268 529-16-8 1.27 2 Tricyclene 8.742 508-32-7 1.54 3 alpha-Thujene 8.892 353313 0.74 4 alpha-Pinene 9.273 7785-70-8 9.55 5 Camphene 9.846 79-92-5 6.39 6 beta-Pinene 10.645 127-91-3 6.70 7 beta-Phellandrene 10.788 555-10-2 3.83 8 3-Carene 11.994 13466-78-9 6.69 9 D-Limonene 12.831 5989-27-5 13.91 10 gamma-Terpinene 13.652 99-85-4 2.23 11 4-Carene 14.539 29050-33-7 2.71 12 alpha-p-Dimethylstyrene 14.692 1195-32-0 0.46 13 Linalool 14.998 78-70-6 0.17 14 Menth-2-en-1-ol 15.819 29803-81-4 0.40 15 Camphor 16.555 21368-68-3 0.24 16 Camphene hydrate 16.821 465-31-6 0.50 17 Borneol 17.412 10385-78-1 1.67 18 Terpinen-4-ol 17.665 20126-76-5 2.85 19 alpha-terpineol 18.083 98-55-5 0.97 20 Fenchyl acetate 18.670 76109-40-5 1.43 21 Linalyl formate 19.530 115-99-1 0.61 22 Bornyl acetate 20.649 5655-61-8 11.63 23 Terpinyl acetate 22.421 80-26-2 6.18 24 Geranyl acetate 23.055 105-87-3 0.23 25 Caryophyllene 24.245 87-44-5 0.62 26 Thujopsene 24.676 470-40-6 0.99 27 alpha-Cubebene 24.880 17699-14-8 0.33 28 beta-Farnesene 24.952 18794-84-8 0.13 29 alpha-Caryophyllene 25.153 6753-98-6 0.11 30 Bicyclosesquiphellandrene 25.322 54324-03-7 0.72 31 beta-Cubebene 25.789 13744-15-5 0.18 32 Isoledene 26.141 95910-36-4 0.63 33 alpha-Curcumene 26.474 644-30-4 0.35 34 gamma-Cadinene 26.668 39029-41-9 0.32 35 Calamenene 26.748 483-77-2 0.16 36 alpha-Himachalene 27.099 3853-83-6 0.41 37 alpha-Elemol 27.497 639-99-6 2.56 38 Nerolidol 27.636 40716-66-3 0.29 39 Cedrol 28.961 77-53-2 0.64 40 gamma-Eudesmol 29.431 1209-71-8 0.98 41 Hinesol 29.618 23811-08-7 0.16 42 alpha-Eudesmol 30.023 473-16-5 1.71 43 alpha-Bisabolol 30.558 515-69-5 0.48 44 Rimuene 35.097 1686-67-5 0.57 45 (+) - 15-Beyerene 35.890 3564-54-3 1.47 46 Bicyclo [9.3.1] pentadeca-3,7-dien-12-ol 36.580 70000-19-0 0.97 97.68

Example 4. Influence of Epithelium, Cryptomeria japonica, and Safflower essential oil on EEG

In Example 4, in order to investigate the effect of inhalation of neurophysiological phenomena on the brainwaves, the 32.4 ㎡-sized laboratory maintained the temperature of 23˚C and the humidity of 50% EEG was measured for 10 subjects, 5 for men and 5 for men.

The EEQ-8 system (LXE3208, LAXTHA Inc. Korea) was used for EEG measurement in Example 4 and LAXTHA data acquisition equipment and analysis program (Telescan) were used to measure the EEG signals of the subjects. The left and right frontal lobes (Fp1, Fp2), left and right frontal lobes (F3, F4), left and right temporal lobes (left) and right temporal lobes T3, T4), attached to the left and right parietal lobes (P3, P4), and attached to the right and left earbones, respectively. A gauze of 3.5 cm in length and 4 cm in length was placed in a paper cup having a diameter of 5 cm, a height of 4.8 cm, and a diameter of 3.5 cm on the top surface. The oil was added to the gauze using a pipette to maintain a distance of about 5 cm from the subject's nose , And subjects were measured 1 minute before incense inhalation (unstimulated) and in inhalation state (incense stimulation) while eyes were closed.

In Example 4, the data processing obtained by EEG measurement of the cerebrospinal fluid, the bamboo tree, and the conical tree essential oil was performed by measuring the EEG signal of the subject for 1 minute, extracting only a typical 30 second EEG signal, The SPSS (SPSS 18.0 version) statistical analysis software program was used to perform a corresponding sample T test.

Table 5 shows the types of EEG indices for analyzing the data obtained before and after inhalation of the untreated, untreated and coniferous essential oils in Example 4, and the meanings of the respective EEG indices are shown in Table 6. Table 5 below shows the types of EEG indices used for data analysis. Table 6 shows the meanings of EEG indices used in data analysis.

No. Analysis index Full name of EEG indicator Waveband (Hz) One AT Absolute Theta Power Spectrum 4 to 8 2 AA Absolute Alpha Power Spectrum 8-13 3 AB Absolute Beta Power Spectrum 13 to 30 4 AG Absolute Gamma Power Spectrum 30 to 50 5 AFA Absolute Fast Alpha Power Spectrum 11-13 6 ASA Absolute Slow Alpha Power Spectrum 8 ~ 11 7 ALB Absolute Low Beta Power Spectrum 12 to 15 8 AMB Absolute Mid Beta Power Spectrum 15-20 9 AHB Absolute High Beta Power Spectrum 20 ~ 30 10 RT Relative Theta Power Spectrum (4 to 8) / (4 to 50) 11 RA Relative Alpha Power Spectrum (8 to 13) / (4 to 50) 12 RB Relative Beta Power Spectrum (13 to 30) / (4 to 50) 13 RG Relative Gamma Power Spectrum (30 to 50) / (4 to 50) 14 RFA Relative Fast Alpha Power Spectrum (11 to 13) / (4 to 50) 15 RSA Relative Slow Alpha Power Spectrum (8 to 11) / (4 to 50) 16 RLB Relative Low Beta Power Spectrum (12 to 15) / (4 to 50) 17 RMB Relative Mid Beta Power Spectrum (15 to 20) / (4 to 50) 18 RHB Relative High Beta Power Spectrum (20 to 30) / (4 to 50) 19 RST Ratio of SMR to Theta (12 to 15) / (4 to 8) 20 RMT (Ratio of Mid Beta to Theta) (15 to 20) / (4 to 8) 21 RSMT Ratio of (SMR ~ Mid Beta) to Theta (12 to 20) / (4 to 8) 22 RAHB Ratio of Alpha to High Beta (8 to 13) / (20 to 30) 23 SEF50 Spectral Edge Frequency 50% 4 to 50 24 SEF 90 Spectral Edge Frequency 90% 4 to 50 25 ASEF Spectral Edge Frequency 50% of Alpha Spectrum Band 8-13

No. Analysis index meaning One AT Absolute power of theta wave (meditation, sleepy) 2 AA Absolute power of Alpha wave (relaxation, stability) 3 AB The absolute power of Beta waves (tension, arousal, activity, cognitive function) 4 AG Absolute power of the Gamma wave (highly cognitive or anxious, excited) 5 AFA Fast alpha Wave's absolute power (comfort, immersion, creativity) 6 ASA Absolute power of Slow alpha wave (relaxation) 7 ALB Absolute power of the low beta wave, SMR parameter (caution or boundary) 8 AMB Mid beta wave absolute power (concentration, attention) 9 AHB High beta Absolute power (stress, mental load, tension) 10 RT Percentage of theta waves in total 11 RA The ratio of alpha waves in total 12 RB Ratio of beta waves in total 13 RG The ratio of gamma waves in total 14 RFA The ratio of fast alpha waves in total 15 RSA The ratio of slow alpha wave in total 16 RLB The ratio of low beta waves in total 17 RMB The ratio of mid beta waves in total 18 RHB The ratio of high beta waves in total 19 RST The ratio of theta wave in SMR wave 20 RMT The ratio of theta wave in mid beta waves 21 RSMT SMR ~ mid beta wave / theta wave (EEG concentration indicator) 22 RAHB Alpha wave / high beta wave (stable and relaxed) 23 SEF50 Median Frequency Brain activity (EEG) 24 SEF 90 Median Frequency Brain activity (mental stress, stress) 25 ASEF Alpha wave SEF50 (comfort: fast alpha waves)

In Example 4, there were significant differences only in the RSMT, RB, RMT, and AFA indices (p <0.05) among the 25 EEG indices, and there was no difference in the remaining indices.

The RSMT index of the subjects increased from 0.412 to 0.544 in the left prefrontal cortex (Fp1) before inhalation by inhalation of the elixir of wood, spruce tree, and coniferous essential oil. The RSMT index (SMR ~ mid beta wave) will be. The results of this study indicate that inhalation of untreated wood, cedar wood, and coniferous essential oil increases concentration.

These results were confirmed by the brain map. It was confirmed that the blue color of the prefrontal region (Fp1) of the left prefrontal region (Fp1) before inhalation of Prunus mulberry, . Brain map shows that the color changes slightly or slightly in other parts of the brain except the left prefrontal area (Fp1) during inhalation and inhalation, but it is significant through T test (FIG. 3, Table 7). Table 7 below shows changes in RSMT indices before inhalation and inhalation of untreated, unspecific, and coniferous essential oils.

RSMT (Ratio of (SMR ~ mid beta) to theta) Site Inhalation N Mean SD t-test p Fp1 Before 10 0.412 0.186 -2.604 0.029 * During 10 0.544 0.295 Fp2 Before 10 0.465 0.233 -1.506 0.166 During 10 0.548 0.295 F3 Before 10 0.457 0.208 -0.727 0.486 During 10 0.496 0.274 F4 Before 10 0.466 0.224 -0.421 0.684 During 10 0.493 0.276 T3 Before 10 0.635 0.285 -1.321 0.219 During 10 0.847 0.568 T4 Before 10 0.750 0.351 -0.028 0.979 During 10 0.754 0.463 P3 Before 10 0.513 0.282 -1.300 0.226 During 10 0.630 0.355 P4 Before 10 0.620 0.319 -0.315 0.760 During 10 0.648 0.327

Fp1: Left prefrontal Fp2: Right prefrontal

F3: Left frontal F4: Right frontal

T3: Left temporal T4: Right temporal

P3: Left parietal P4: Right parietal

The relative beta power spectrum (RB) indicator represents the ratio of beta waves in the whole brain wave spectrum. EEG values were significantly increased in the left prefrontal region (Fp1) (p <0.05) (Table 7). These results were confirmed by the brain map, and it was confirmed that the blue color of the prefrontal region (Fp1) of the left prefrontal region (Fp1) before inhalation of Prunus maxima, Inferred. Brain map shows that the color changes slightly or slightly in other parts of the brain except the left prefrontal area (Fp1) during inhalation and inhalation, but it is significant through T test (Fig. 4 and Table 8). Table 8 below shows changes in the RB index before inhalation and inhalation of untreated, untreated, and coniferous essential oils.

RB (Ralative beta power spectrum) Site Inhalation N Mean SD t-test p Fp1 Before 10 0.173 0.074 -2.582 0.030 * During 10 0.197 0.089 Fp2 Before 10 0.191 0.090 -0.241 0.815 During 10 0.194 0.080 F3 Before 10 0.174 0.079 -0.586 0.572 During 10 0.181 0.079 F4 Before 10 0.167 0.079 -0.999 0.344 During 10 0.178 0.077 T3 Before 10 0.261 0.090 -0.008 0.994 During 10 0.261 0.090 T4 Before 10 0.273 0.078 0.915 0.384 During 10 0.254 0.077 P3 Before 10 0.187 0.080 -1.342 0.213 During 10 0.201 0.085 P4 Before 10 0.194 0.081 -0.039 0.970 During 10 0.194 0.082

Fp1: Left prefrontal Fp2: Right prefrontal

F3: Left frontal F4: Right frontal

T3: Left temporal T4: Right temporal

P3: Left parietal P4: Right parietal

(P <0.05) (Table 9). The RMT index was significantly higher than that of the whole brain wave spectrum (p <0.05) mid beta means the ratio of theta wave. The increase in the RMT index due to inhaling the elixir wood, spruce tree, and conifer tree essential oil is deduced to be due to the increase of beta wave. These results were confirmed by the brain map. It was confirmed that the purple color of the left prefrontal region (Fp1) before inhalation of the saphenous tree, Cryptomeria japonica, and conical tree essential oil changed to blue by inhalation of essential oil (Fig. 5). Table 9 below shows the change in RMT index before inhalation and inhalation of untreated wood, saplings and coniferous essential oils.

Ratio of mid beta to theta (RMT) Site Inhalation N Mean SD t-test p Fp1 Before 10 0.216 0.100 -2.406 0.039 * During 10 0.306 0.207 Fp2 Before 10 0.242 0.118 -1.414 0.191 During 10 0.294 0.189 F3 Before 10 0.238 0.111 -0.936 0.374 During 10 0.273 0.190 F4 Before 10 0.231 0.101 -0.971 0.357 During 10 0.268 0.181 T3 Before 10 0.363 0.160 -1.074 0.311 During 10 0.496 0.409 T4 Before 10 0.383 0.151 -0.637 0.540 During 10 0.453 0.341 P3 Before 10 0.238 0.110 -1.581 0.148 During 10 0.329 0.237 P4 Before 10 0.273 0.113 -0.952 0.366 During 10 0.319 0.200

Fp1: Left prefrontal Fp2: Right prefrontal

F3: Left frontal F4: Right frontal

T3: Left temporal T4: Right temporal

P3: Left parietal P4: Right parietal

The AFA (absolute fast alpha power spectrum) index is an indicator of the absolute power of the fast alpha wave, and the inhalation of the elixir wood, spruce tree, and cone tree essential oil is statistically significant in comparison with the pre-inhalation index in the left temporal area (T3) (P <0.05), respectively (Table 10). These results were confirmed by the brain map. It was confirmed that purple color of the left temporal region (T3) before inhalation of the elderly tree, spruce tree, and conical tree essential oil changed to green by essential oil inhalation (FIG. 6). Table 10 below shows changes in the AFA index during inhalation and inhalation of untreated wood, cinnabar, and coniferous essential oils.

Absolute fast alpha power spectrum (AFA) Site Inhalation N Mean SD t-test p Fp1 Before 10 6.578 4.924 0.052 0.960 During 10 6.552 4.587 Fp2 Before 10 6.578 5.706 -0.555 0.592 During 10 6.969 4.707 F3 Before 10 6.973 5.021 -1.153 0.279 During 10 7.716 5.535 F4 Before 10 7.406 6.213 -0.695 0.505 During 10 7.706 5.898 T3 Before 10 3.992 2.698 -2.346 0.044 * During 10 4.822 3.561 T4 Before 10 7.013 4.999 0.899 0.392 During 10 6.632 4.362 P3 Before 10 9.480 7.468 -0.850 0.417 During 10 10.191 7.051 P4 Before 10 10.700 9.187 -1.212 0.256 During 10 12.782 10.548

Fp1: Left prefrontal Fp2: Right prefrontal

F3: Left frontal F4: Right frontal

T3: Left temporal T4: Right temporal

P3: Left parietal P4: Right parietal

The results of Example 4 show that the RSMT index (in the prefrontal area (Fp1), which controls the general arousal state of the cerebral cortex and the most complicated goal-directed behavior and attention of the human cortex when inhaled, , RB index, and RMT index. The results of RSMT, RB, and RMT indicators are all due to the increase of beta waves, and the brain is considered to be activated when attention is paid to inhaling the elixir of wood, bamboo tree, and conifer tree essential oil.

Claims (4)

As an active ingredient, it contains an essential oil extracted from elongated leaves, cervical leaves, and coniferous leaves,
The essential oil is extracted from a mixture of the perennial, perennial, and perennial tree leaves, and the essential oil is extracted from the perennials, perennials, and perennials by an error weight ratio of 5% By weight in a weight ratio of 1: 1: 1,
The relationship between the active ingredient and the content of each ingredient in the essential oil is D-Limonene> Bornyl acetate>alpha-Pinene>beta-Pinene>3-Carene>Camphene> Terpinyl acetate>beta-Phellandrene>
The essential oil has a ratio of (SMR to Mid beta) to theta), a relative beta power spectrum (RB) index and a ratio of mid beta to theta (RBT) index at the left prefrontline (Fp1) ) Index, and an absolute fast alpha power spectrum (AFA) index at the left temporal lobe (T3) site. delete The method according to claim 1,
The essential oil is selected from the group consisting of (+) - 15-Beyerene, alpha-Bisabolol, 3-Carene, 4-Carene, alpha-Caryophyllene, alpha-Cubebene, alpha-Curcumene, Bicyclo [9.3.1] pentadeca-3,7-dien- beta-Cubebene, alpha-p-dimethylstyrene, alpha-Elemol, alpha-Eudesmol, gamma-Eudesmol, beta, beta-cysteine, beta-cysteine, bicyclosesquiphellandrene, Borneol, Bornyl acetate, gamma-Cadinene, Calamenene, Camphene, Camphene hydrate, Camphor, Caryophyllene 2-en-1-ol, Nerolidol, beta-Phellandrene, alpha-Pinene, beta-Pinene, Linalool, Linalyl formate, Wherein the composition comprises Rimuene, Santene, Terpinen-4-ol, alpha-Terpineol, gamma-Terpinene, Terpinyl acetate, alpha-Thujene, Thujopsene, and Tricyclene compounds. A wood applied or immersed with the composition of claim 1 or 3,
The wood is continuously exposed to the essential oil and has a ratio of (SMR to Mid beta) to theta index, a relative beta power spectrum (RBM) index and a ratio of mid beta (RMT) index at the left prefrontal (Fp1) to theta index, and an absolute fast alpha power spectrum (AFA) index at the left temporal lobe (T3) site, indicating a mental concentration effect.

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