KR102506628B1 - Aqua green Abies koreana aroma composition for enhancing comfort and concentration of human brain - Google Patents

Abstract

The present invention manufactures a fragrance composition for increasing the comfort and concentration of the human brain, a health functional food and cosmetic composition for increasing the comfort and concentration of the human brain containing the fragrance composition, and a fragrance mixture for increasing the comfort and concentration of the human brain. It relates to a method and a perfume mixture for increasing the comfort and concentration of the human brain prepared by the method, and the perfume composition of the present invention reduces AT in right prefrontal lobe, left frontal lobe, right frontal lobe, left parietal lobe and right parietal lobe, right frontal lobe , It induces an increase in RST in the right temporal lobe, left parietal lobe and right parietal lobe, an increase in RSMT in the left temporal lobe, and an increase in ASEF in the left parietal lobe, thereby making the human brain pleasant and improving concentration.

Description

Aqua green Abies koreana aroma composition for enhancing comfort and concentration of human brain}

The present invention manufactures a fragrance composition for increasing the comfort and concentration of the human brain, a health functional food and cosmetic composition for increasing the comfort and concentration of the human brain containing the fragrance composition, and a fragrance mixture for increasing the comfort and concentration of the human brain. It relates to a method and a flavor mixture for increasing the comfort and concentration of the human brain prepared by the method.

Perfume is a product obtained by diluting essential oils, a raw material for volatile fragrance compounds, with ethanol. Natural flavors are divided into animal flavors obtained from animals and plant flavors obtained from plants. Animal spices include ambergris from humpback whales, musk from civet deer, civette from civet cats, and castorium from beavers, but these days they are not used well. Plant spices can be obtained by distilling, extracting, or pressing flowers, leaves, fruits, seeds, resins, roots, and stems of about 1,500 species of plants, but their purity is low, and their fragrance changes greatly during extraction and distillation. There are severe changes in strength and quality depending on the region, harvest time, and post-harvest storage method, and since supply uncertainty is large, about 200 species are currently on the market.

Organic synthetic fragrances can be obtained through organic synthesis in the laboratory. They are more pure than natural fragrances, are cheaper to use, stable, have a longer shelf life, have a higher concentration, and are preferred in the fragrance industry because there is no problem with seasonal supply. . A representative example of organic synthetic perfume is aldehyde used in Chanel No.5.

Each of the spices represents a unique and unique fragrance, which is called a fragrance or note. Fragrance notes are generally citrus, floral, green, fruity, aldehyde, oriental, spicy, woody, cypre, and after. It is represented by fougere, marine, and gourmand. When several spices are mixed, the scent of each spice changes. Combining several single spice products is called blending, and through this, a combination spice product with various fragrances can be created. Combination spices are divided into fragrance (aromatic flavoring) and flavor (food flavoring) according to their use. Depending on the content of essential oils, fragrances are classified into perfume (15-30%), eau de parfum (15-18%), eau de toilette (4-8%), and eau de cologne (3-5%). Separated. In general, when trying to create a new fragrance product by mixing mixed spices, the top note that the fragrance immediately appears when sprayed, the middle note that expresses the characteristics of the fragrance well, and the last note that gives depth and volume to the fragrance must be in harmony with each other.

When a human inhales perfume, the fragrance molecules move along the nasal cavity and combine with olfactory receptors to generate an electrical signal. When the electrical signal generated at this time is transmitted to the cerebral cortex along the olfactory tract in the olfactory bulb, brain activation is performed. Odor molecules contained in spices are known to affect the human brain, change brain waves, and induce physiological and psychological changes.

When the cerebral cortex is activated by a certain stimulus, brain waves are generated, which are electrical signals generated between neurons in the nervous system of the human cerebral cortex. The cerebrum occupies more than 75% of the total human brain volume and is divided into left and right hemispheres. The cerebral cortex, the surface of the cerebrum, has a wrinkled shape with a round protruding gyrus and a grooved sulcus.

Electrodes that can amplify cerebral electrical activity are attached to the scalp, the obtained waveforms are recorded and analyzed as a graph, and EEG, an electrical signal generated by the autonomous activity of the brain, records and observes brain activity to improve human brain performance. It is used as an indicator of human psychophysiological functions such as arousal, activity, anxiety and stability. EEG is used in cognitive science, medicine, pharmacy, and criminal investigation because it has the advantage of being able to record and measure objectively, non-invasively, and continuously.

EEG mainly shows a frequency of 0 to 60 Hz and an amplitude of 5 to 300 μV. Depending on the frequency range, delta waves (0 to 4 Hz), theta waves (4 to 8 Hz), alpha waves (8 to 13 Hz), beta waves It is divided into waves (13-30 Hz) and gamma waves (30-50 Hz). Delta waves and theta waves are slow waves and appear when thinking and working using information inside the brain. Beta waves higher than alpha waves are fast waves. In EEG analysis, relative power is used to reduce individual differences such as difference in thickness of the skull, electrical state of the scalp, and tension, as well as absolute power, and to analyze an accurate EEG response.

Korean Patent Publication No. 2015-0057335 discloses a composition for suppressing harvesting containing a ficus extract, and Korean Patent No. 2020384 discloses a cosmetic composition for skin whitening and wrinkle improvement containing essential oil derived from ficus ficus. , It is different from the present invention in specific active ingredients and effects.

The present invention has been devised by the above needs, and in the present invention, by using the essential oil extracted by steam distillation of Korean fir tree and natural flavors and synthetic flavors suitable for various scent images, AT (absolute theta power spectrum) in a specific part of the brain. decrease, RST (Ratio of SMR to theta power spectrum), RSMT (Ratio of (SMR~mid beta) to Theta power spectrum) and ASEF (spectral edge frequency 50% of alpha spectrum band) increase, thereby inducing human brain It is an object of the present invention to provide an aqua green fragrance composition that is pleasant and increases concentration.

In order to solve the above problems, the present invention is aldehyde C14 (Aldehyde C14), aldehyde C16 (Aldehyde C16), benzyl acetate, bergamot essential oil, Calone, cis-3-hexenol (cis -3 Hexenol), Citral, Dipropylene glycol, Hedion, Hydroxy citronellal, Lemon essential oil, Lilial, Linalool ( Linalool), Methyl anthranilate, Phenyl ethyl alcohol, Sandalwood essential oil, Tonalide, Verdox, and a spice mixture mixed with Korean locust extract are effective. It reduces AT (absolute theta power spectrum) in right frontal lobe, left frontal lobe, right frontal lobe, left parietal lobe and right parietal lobe, and RST (Ratio of SMR to theta power spectrum) in right frontal lobe, right temporal lobe, left parietal lobe and right parietal lobe. power spectrum), increase RSMT (Ratio of (SMR~mid beta) to Theta power spectrum) in the left temporal lobe, and increase ASEF (spectral edge frequency 50% of alpha spectrum band) in the left parietal lobe It provides a fragrance composition for increasing the comfort and concentration of the human brain.

In addition, the present invention provides a health functional food for increasing the comfort and concentration of the human brain containing the flavor composition.

In addition, the present invention provides a cosmetic composition for increasing the comfort and concentration of the human brain comprising the above fragrance composition.

In addition, the present invention is aldehyde C14 (Aldehyde C14), aldehyde C16 (Aldehyde C16), benzyl acetate, bergamot essential oil, Calone, cis-3-hexenol, Citral, Dipropylene glycol, Hedion, Hydroxy citronellal, Lemon essential oil, Lilial, Linalool, Methyl anthra It is prepared by mixing Methyl anthranilate, Phenyl ethyl alcohol, Sandalwood essential oil, Tonalide, Verdox and Korean fir tree extract, and is used for right frontal lobe, left frontal lobe, It decreased absolute theta power spectrum (AT) in the right frontal lobe, left parietal lobe and right parietal lobe, increased the Ratio of SMR to theta power spectrum (RST) in the right frontal lobe, right temporal lobe, left parietal lobe and right parietal lobe, and RSMT in the left temporal lobe. (Ratio of (SMR~mid beta) to Theta power spectrum) and fragrance for improving concentration and comfort of the human brain, characterized by increasing ASEF (spectral edge frequency 50% of alpha spectrum band) in the left parietal lobe A method for preparing the mixture is provided.

In addition, the present invention provides a flavor mixture for increasing the comfort and concentration of the human brain prepared by the above method.

The combined flavor of the present invention reduces AT index (absolute theta power spectrum) in a specific brain region, RST index (Ratio of SMR to theta power spectrum), RSMT index (Ratio of (SMR-mid beta) to Theta power spectrum) , ASEF index (spectral edge frequency 50% of alpha spectrum band) is increased to increase brain comfort and concentration, so it can be used to develop various fragrance industry products for consumers who want comfort and concentration. .

1 is a photograph showing the 10-20 International Standard Electrode Attachment Method, which presents a site where electrodes are attached to the human brain for EEG measurement.
2 is a photograph showing a state in which a cap for electroencephalogram (EEG) is put on the brain of a subject and an EEG is measured.

In order to achieve the object of the present invention, the present invention is aldehyde C14 (Aldehyde C14), aldehyde C16 (Aldehyde C16), benzyl acetate, bergamot essential oil, Calone, cis-3-hexenol (cis-3 Hexenol), Citral, Dipropylene glycol, Hedion, Hydroxy citronellal, Lemon essential oil, Lilial, Lina Flavor blend of Linalool, Methyl anthranilate, Phenyl ethyl alcohol, Sandalwood essential oil, Tonalide, Verdox and Ficus extract. It reduces AT (absolute theta power spectrum) in the right frontal lobe, left frontal lobe, right frontal lobe, left parietal lobe and right parietal lobe, and RST (Ratio of SMR) in the right frontal lobe, right temporal lobe, left parietal lobe and right parietal lobe, containing as an active ingredient to theta power spectrum), RSMT (Ratio of (SMR~mid beta) to Theta power spectrum) in the left temporal lobe, and ASEF (spectral edge frequency 50% of alpha spectrum band) in the left parietal lobe. It provides a fragrance composition for increasing the comfort and concentration of the human brain.

In the perfume composition of the present invention, the perfume composition preferably contains 1.2 to 1.8% by weight of aldehyde C14, 2.2 to 2.8% by weight of aldehyde C16, 3.5 to 4.5% by weight of benzyl acetate, Bergamot essential oil 12-14% by weight, Calone 2.7-3.3% by weight, cis-3-Hexenol 7-8% by weight, Citral 1.8-2.2% by weight, Dipropylene glycol 14-16 wt%, Hedion 6.5-7.5 wt%, Hydroxy citronellal 4.5-5.5 wt%, Lemon essential oil 4.5-5.5 wt%, Lilial 2.2-2.8 wt%, Linalool 7-7.5 wt%, Methyl anthranilate 1-1.5 wt%, Phenyl ethyl alcohol 3.2-3.8 wt%, It may consist of 4.5 to 5.5% by weight of essential oil of Sandalwood, 6.5 to 7.5% by weight of Tonalide, 4.5 to 5.5% by weight of Verdox and 2.7 to 3.3% by weight of Korean fir tree extract,

More preferably, 1.5% by weight of Aldehyde C14, 2.5% by weight of Aldehyde C16, 4% by weight of benzyl acetate, 13% by weight of essential oil of Bergamot, 3% by weight of Calone 7.5% by weight of cis-3 Hexenol, 2% by weight of Citral, 15% by weight of Dipropylene glycol, 7% by weight of Hedion, hydroxycitro Hydroxy citronellal 5% by weight, Lemon essential oil 5% by weight, Lilial 2.5% by weight, Linalool 7.25% by weight, Methyl anthranilate 1.25% by weight, phenyl ethyl It may be composed of 3.5% by weight of phenol (Phenyl ethyl alcohol), 5% by weight of Sandalwood essential oil, 7% by weight of Tonalide, 5% by weight of Verdox, and 3% by weight of Korean fig tree extract.

The AT power spectrum means the absolute power spectrum value of the theta wave region, and a decrease in AT means that tension is relieved and attention is increased. RST refers to the ratio of SMR waves to Theta waves, and an increase in RST means an increase in attentional concentration. In addition, RSMT means SMR~Mid Beta wave/Theta wave, and is used as a neurophysiological brain wave concentration indicator. An increase in ASEF is an increase in the ratio of fast alpha waves in the entire brain wave, and when fast alpha waves increase, the brain is awakened and concentration is improved, while a sense of stability and comfort can be felt.

The present invention also provides a health functional food for increasing the comfort and concentration of the human brain containing the flavor composition of the present invention.

The health functional food includes various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, coloring agents and enhancers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, It may contain pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, and the like. In addition, it may contain fruit flesh for the production of natural fruit juice and vegetable beverages. These components may be used independently or in combination.

The health functional food of the present invention may contain various flavoring agents or natural carbohydrates as additional ingredients. The natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as thaumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used.

The present invention also provides a cosmetic composition for increasing the comfort and concentration of the human brain comprising the fragrance composition of the present invention.

The cosmetic composition includes softening lotion, astringent lotion, nutrient lotion, nutrient lotion, nutrient essence, nutrient cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, gel, powder, and makeup base. , It is preferable to have a formulation selected from the group consisting of body lotion, body cream, body oil, body essence, lotion, ointment, gel, cream, patch and spray, but is not limited thereto.

The present invention also relates to Aldehyde C14, Aldehyde C16, Benzyl acetate, Bergamot essential oil, Calone, cis-3-Hexenol, Citral, Dipropylene glycol, Hedion, Hydroxy citronellal, Lemon essential oil, Lilial, Linalool, Methyl anthra It is prepared by mixing Methyl anthranilate, Phenyl ethyl alcohol, Sandalwood essential oil, Tonalide, Verdox and Korean fir tree extract, and is used for right frontal lobe, left frontal lobe, It decreased absolute theta power spectrum (AT) in the right frontal lobe, left parietal lobe and right parietal lobe, increased the Ratio of SMR to theta power spectrum (RST) in the right frontal lobe, right temporal lobe, left parietal lobe and right parietal lobe, and RSMT in the left temporal lobe. (Ratio of (SMR~mid beta) to Theta power spectrum) and fragrance for improving concentration and comfort of the human brain, characterized by increasing ASEF (spectral edge frequency 50% of alpha spectrum band) in the left parietal lobe A method for preparing the mixture is provided.

More specifically, the method for producing a spice mixture for increasing the comfort and concentration of the human brain of the present invention contains 1.2 to 1.8 wt% of aldehyde C14 and 2.2 to 1.8 wt% of aldehyde C16, based on the total weight of the spice mixture. 2.8 wt%, Benzyl acetate 3.5-4.5 wt%, Bergamot essential oil 12-14 wt%, Calone 2.7-3.3 wt%, cis-3-Hexenol 7 ~8 wt%, Citral 1.8~2.2 wt%, Dipropylene glycol 14~16 wt%, Hedion 6.5~7.5 wt%, Hydroxy citronellal 4.5 ~5.5 wt%, Lemon essential oil 4.5~5.5 wt%, Lilial 2.2~2.8 wt%, Linalool 7~7.5 wt%, Methyl anthranilate 1~1.5 wt% %, Phenyl ethyl alcohol (Phenyl ethyl alcohol) 3.2 ~ 3.8% by weight, Sandalwood essential oil 4.5 ~ 5.5% by weight, Tonalide 6.5 ~ 7.5% by weight, Verdox 4.5 ~ 5.5% by weight and It can be prepared by mixing 2.7 to 3.3% by weight of Korean fig tree extract,

More specifically, based on the total weight of the fragrance mixture, 1.5% by weight of Aldehyde C14, 2.5% by weight of Aldehyde C16, 4% by weight of benzyl acetate, 13% by weight of Bergamot essential oil, Calone 3% by weight, cis-3-hexenol 7.5% by weight, Citral 2% by weight, Dipropylene glycol 15% by weight, Hedion 7 Weight %, Hydroxy citronellal 5% by weight, Lemon essential oil 5% by weight, Lilial 2.5% by weight, Linalool 7.25% by weight, Methyl anthranilate ) 1.25% by weight, 3.5% by weight of phenyl ethyl alcohol, 5% by weight of Sandalwood essential oil, 7% by weight of Tonalide, 5% by weight of Verdox and 3% by weight of Korean fir tree extract It can be prepared by mixing %.

The present invention also provides a flavor mixture for increasing the comfort and concentration of the human brain prepared by the above method.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only to illustrate the present invention, and the content of the present invention is not limited to the following examples.

1. Sample acquisition and storage

In the case of ficus ficus essential oil used in the present invention, ficus ficus samples were collected from the ficus ficus colony in Namjeon 1-ri, Inje-gun, Gangwon-do. After being transferred to Kangwon National University natural product laboratory immediately after collection, essential oil extraction was carried out while storing at 4℃ in a low-temperature warehouse (2nd floor of Agricultural Building No. 3) at the Institute of Agriculture and Life Sciences, Kangwon National University.

The perfume composition of the present invention used to develop the Korean fig tree perfume contains Aldehyde C14, Aldehyde C16, Benzyl acetate, Bergamot essential oil, Calone, cis-3- Hexenol, Citral, Dipropylene glycol, Hedion, Hydroxy citronellal, Lemon essential oil, Lilial , Linalool, Methyl anthranilate, Phenyl ethyl alcohol, Sandalwood essential oil, Tonalide, Verdox are Cas Number Samples with clearly specified were purchased from a domestic raw material supplier.

2. Extraction of natural essential oil of Korean locust tree

Extraction of essential oil from fir tree samples was performed using a steam distillation apparatus (EssenLab-Plus, HANIL LABTECH, Korea). 5 L of distilled water was put in the steam generating part of the steam distillation device, 1 kg of fir tree sample was put in the essential oil generating part, and 2℃ cooling water was continuously flowed through the cooling pipe. Steam was generated while maintaining the temperature of the steam generating part at 100 ° C for 90 minutes.

When essential oil was produced for 15 minutes, 30 minutes, 45 minutes, 60 minutes, 75 minutes, and 90 minutes from the start of the experiment, the recovery rate was 013%, 017%, 016%, 012%, 010%, and 009%, respectively. The production of Korean fir tree essential oil was maximum at 30 minutes after the start of the experiment, and showed a tendency to decrease gradually thereafter.

After completion of the experiment, in order to collect only pure essential oil, the essential oil layer was passed through a triangular funnel containing anhydrous sodium sulfate to remove moisture and impurities, and the collected essential oil was stored in a refrigerator at 4 ° C.

Example 1. Flavor evaluation of Korean locust tree natural essential oil

The fragrance evaluation of the obtained locust tree essential oil was performed by 5 expert panelists from the Rose of Sharon, a perfume development club at Kangwon National University, who had been trained in the sense of smell for more than 6 months. After soaking the extracted natural essential oil solution (100%) on a scent paper (0.5 cm × 5 cm), place the scent paper at a position of 5 cm from the nose of the panel to smell the scent and evaluate the characteristics of the scent using the fragrance evaluation term ( It was described using Table 2). The fragrance types used in the sensory evaluation were aldehyde, animalic, balsamic, citrus, coniferous, earthy, floral, and fruity. , green, herbal, medicinal, minty, marine, mossy, musky, oily, oriental, powdery (powdery), smoky (smoky), spicy (spicy), sweet (sweet), woody (woody), and commonly used by the fragrance evaluation expert group.

Fragrance types and characteristics of essential oils dreadlocks type characteristic example Aldehyde Oil or fat-like aroma Aldehyde C9 Animalic incense with animal odor Civet cat oil Balsamic Sweet and warm feeling like normal vanilla Benzoin Citrus Refreshing citrus scent, sweet and fresh feeling Bergamot Coniferous Refreshing and clean pine scent Cajuput Earthy Unique soil scent in the air when it rains Vetiver Floral The scent of a single flower or a bouquet of flowers Jasmin, Rose Fruity Fragrance expressing peach, strawberry, tropical fruit, etc. Apple, Plum Green Fragrance felt when the leaves are cut or rubbed Galbanum Herbal A strong, pungent plant scent, such as the scent of mugwort Lavender Medicinal scent reminiscent of medicine Anis Minty Menthol flavor of peppermint or spearmint Peppermint Marine Slightly salty scent reminiscent of the cool sea Calone Wet Moist fragrance with an aqua-type scent Musky Musk-like incense Musk Oily Oily scent like olive oil Olive Oriental Mysterious incense that is widely supplied in the Orient Opopanax Powdery Spices that make you feel the powder Heliotropine Smokey Smoke-like smell from smoked fish, etc. Spicy The pungent scent of spices Clove Sweet Sweet, fragrant, pleasurable feeling Ethyl maltol Woody Subtle and profound woody scent from trees Sandalwood

The fragrance evaluation results of the essential oil extracted using a steam distillation are shown in Table 2 below.

Fragrance type of fir tree essential oil Fragrance Type Abies koreana Wilson. Aldehyde Animalic Balsamic Citrus ● Coniferous ● Earthy ● Floral Fruity Green ● Herbal ● Medicinal Minty ● Marine Wet ● Musky Oily ● Oriental Powdery Smokey ● Spicy ● Sweet Woody ●

Example 2. Development of perfume composition using natural essential oil of Korean locust tree and investigation of consumer preference

Essential oil extracted from the locust tree by steam distillation, Aldehyde C14, Aldehyde C16, Benzyl acetate, Bergamot essential oil, Calone, cis-3-hexenol (cis -3 Hexenol), Citral, Dipropylene glycol, Hedion, Hydroxy citronellal, Lemon essential oil, Lilial, Linalool ( Linalool), methyl anthranilate, phenyl ethyl alcohol, Sandalwood essential oil, Tonalide, and Verdox were combined to develop four new fragrance combinations. (Table 3). Korean locust essential oil has its own unique fragrance and showed a very new fragrance when mixed with perfume compositions.

The fragrance composition of the present invention is a combination of aqua green (aqua green) series, characterized by giving a scent like clean air, reminiscent of the image of a fir tree and the sky as a motif, and a green fir tree under a blue sky. It is a spice.

Composition and composition ratio for the development of new flavors flavoring ingredients One 2 3 4 One Aldehyde C14 1.50 1.00 1.00 1.50 2 Aldehyde C16 2.50 2.50 2.00 3.00 3 Benzyl acetate 4.00 5.00 4.00 5.00 4 Bergamot 13.00 13.00 10.00 10.00 5 Calone 3.00 3.00 3.00 3.00 6 cis-3 Hexenol 7.50 5.50 5.50 7.50 7 Citral 2.00 2.00 2.00 2.00 8 Dipropylene glycol (DPG) 15.00 15.00 15.00 15.00 9 Hedion 7.00 6.00 5.00 8.00 10 Hydroxy citronellal 5.00 6.00 7.00 4.00 11 Lemon 5.00 5.00 5.00 5.00 12 Lilial 2.50 2.50 2.50 2.50 13 Linalool 7.25 7.00 7.00 7.00 14 Methyl anthranilate 1.25 1.25 1.25 1.25 15 Phenyl ethyl alcohol (PEA) 3.50 3.25 3.25 3.25 16 Sandalwood 5.00 5.00 5.50 5.00 17 Tonalide 7.00 7.00 7.00 7.00 18 Verdox 5.00 3.00 5.00 3.00 19 Korean fig tree extract (100%) 3.00 7.00 9.00 7.00 Sum 100 100 100 100

The preference survey for the four flavor combinations was conducted at Kangwon National University College of Agriculture and Life Science Building No. 2, College of Agriculture and Life Science Building No. 3, Eco-friendly Center, and Mirae Plaza, and a total of 100 people were surveyed. After soaking the undiluted solution (100%) of each combination spice on a test paper (0.5 cm × 5 cm), place the test paper at a position of 5 cm from the nose of the panel, smell it, and score 10 points for the most preferred flavor and 10 points for the most non-preferred flavor. was given 1 point. An average value was obtained based on the preference results, and it is shown in Table 4.

Consumer Preference for 4 Types of Spices consumer preference Mixed Spices 1 Mixed Spices 2 Combination Spices 3 Combination Spices 4 medium 8.6 5.0 6.3 7.1

As a result of a preference survey on four types of perfume composition, consumers preferred the composition of perfume combination 1 the most. Combination spice 2 had the lowest preference.

Example 3. Effect of Aqua Green Fragrance Combination Fragrance 1 on Human EEG

Through Example 2, electroencephalogram (EEG) was measured to find out what effect it has on human brain activity when inhaling spice 1, which had the highest consumer preference. EEG measurements were performed on a total of 20 subjects, 10 males and 10 females, in a 32.4 m ² laboratory maintained at a temperature of 23°C and a humidity of 50%, free of noise as much as possible.

The EEG measuring device used for EEG measurement was the Quick-30 Dry EEG Headset (Cognionics, Inc. USA), Cognionics' Cognionics Data Acquisition Software was used to measure the participant's EEG signal, and LAXTHA's data collection equipment and analysis were used for EEG data analysis. A program (Telescan) was used. The experiment participants were asked to wear an EEG cap with 29 ground electrodes distributed using the International 10~20 System, and the ground electrodes were attached to each EEG part using Flex Sensors (Cognionics, Inc. USA). Also, a reference electrode was attached to the left earlobe using Drypad Sensors.

The air cylinder outlet was placed 5 cm in front of the participant, and measurements were taken for a total of 120 seconds (2 minutes), 60 seconds each before inhalation (non-stimulus) and inhalation (scent stimulation).

Data processing obtained through EEG measurement was performed by extracting only representative EEG signals for 50 seconds among the EEG signals of the experiment participants, performing batch processing, and then using SPSS (SPSS 24.0 version), a statistical analysis software program. A paired-sample T test was performed. In this Example, the types of EEG indicators for data analysis obtained before (non-stimulation) and during inhalation (scent stimulation) of aqua green fragrance are shown in Table 5, and the meaning of each EEG indicator is shown in Table 6.

Types of EEG indicators used for data analysis No. Analysis indicator Full name of EEG analysis index Wavelength (Hz) One AT Absolute Theta Power Spectrum 4 to 8 2 AA Absolute Alpha Power Spectrum 8 to 13 3 AB Absolute Beta Power Spectrum 13 to 30 4 AG Absolute Gamma Power Spectrum 30 to 50 5 AFA Absolute Fast Alpha Power Spectrum 11~13 6 ASA Absolute Slow Alpha Power Spectrum 8-11 7 ALB Absolute Low Beta Power Spectrum 12 to 15 8 AMB Absolute Mid Beta Power Spectrum 15 to 20 9 AHB Absolute High Beta Power Spectrum 20 to 30 10 RT Relative Theta Power Spectrum (4~8)/(4~50) 11 RA Relative Alpha Power Spectrum (8~13)/(4~50) 12 RB Relative Beta Power Spectrum (13-30)/(4-50) 13 RG Relative Gamma Power Spectrum (30~50)/(4~50) 14 RFA Relative Fast Alpha Power Spectrum (11~13)/(4~50) 15 RSA Relative Slow Alpha Power Spectrum (8~11)/(4~50) 16 RLB Relative Low Beta Power Spectrum (12~15)/(4~50) 17 RMB Relative Mid Beta Power Spectrum (15~20)/(4~50) 18 RHB Relative High Beta Power Spectrum (20~30)/(4~50) 19 RST Ratio of SMR to Theta (12~15)/(4~8) 20 RMT Ratio of Mid Beta to Theta (15~20)/(4~8) 21 RSMT Ratio of (SMR-Mid Beta) to Theta (12~20)/(4~8) 22 RAHB Ratio of Alpha to High Beta (8~13)/(20~30) 23 SEF50 Spectral Edge Frequency 50% 4 to 50 24 SEF90 Spectral Edge Frequency 90% 4 to 50 25 ASEF Spectral Edge Frequency 50% of Alpha Spectrum Band 8 to 13

Meaning of EEG indices used in data analysis No. Analysis indicator meaning One AT Absolute power of Theta waves (meditation, when sleepy) 2 AA Alpha wave absolute power (relaxation, stability) 3 AB Absolute power of beta wave (tension, arousal, activation, cognitive function) 4 AG Absolute power of gamma waves (high level of cognitive function or anxiety or excitement) 5 AFA Absolute power of fast alpha waves (comfort and immersion) 6 ASA Absolute power of slow alpha wave (relaxation) 7 ALB Absolute power of low beta waves, also called SMR waves (attention or alertness) 8 AMB Absolute power of mid beta waves (concentration, attention) 9 AHB Absolute power of high beta waves (stress, mental load, tension) 10 RT Percentage of theta waves in the total 11 RA Percentage of Alpha waves in the total 12 RB The ratio of beta waves to the total 13 RG Ratio of gamma waves to the total 14 RFA Percentage of Fast Alpha waves in total 15 RSA Percentage of Slow Alpha waves in the total 16 RLB Percentage of Low Beta waves in the total 17 RMB Percentage of Mid Beta waves in the total 18 RHB Percentage of High Beta waves in the total 19 RST Ratio of Theta waves in SMR waves in total 20 RMT Ratio of Mid Beta to Theta waves in total 21 RSMT SMR~Mid Beta wave/Theta wave (brain wave concentration index) 22 RAHB Alpha wave/High Beta wave (stabilization and relaxation) 23 SEF50 Median Frequency brain activity (brain wave arousal) 24 SEF90 Median Frequency brain activity (mental load, stress) 25 ASEF SEF50 of Alpha wave (comfort: there are many Fast Alpha waves)

As a result of analyzing the effect of mixed spice 1, which was the most preferred in Example 2 of the present invention, on human brain waves, only the AT index, RST index, RSMT index and ASEF index among 25 indexes were found to be statistically significant. ( p <0.05) Only this will be explained.

1. absolute theta power spectrum (AT)

Inhalation of the combination flavor 1 reduced the participants' AT index in the right prefrontal lobe (Fp2), left and right frontal lobes (F3, F4), and left and right parietal lobes (P3, P4) (Table 7). Theta waves have a frequency of 4 Hz or more and less than 8 Hz and an amplitude of 30 μV or less, and appear during brain activity in static states such as drowsy or light sleep, mild consciousness disorder, and deep meditation. It is known that activity decreases when acquiring new knowledge or when paying attention. Since the AT power spectrum was reduced by inhalation of the combination spice 1, it can be seen that the concentration of attention increased while the combination spice 1 relieved tension.

Changes in AT index due to inhalation of blended fragrance 1 absolute theta power spectrum (AT) brain location inhalation state number of subjects average Standard Deviation t-test p left prefrontal lobe
(Fp1) Before 20 11.7678 1.2646 2.0262 0.0570 During 20 10.8857 1.1147 right prefrontal lobe
(Fp2) Before 20 11.6228 1.4907 2.3494 0.0298 During 20 10.4048 1.2349 Left frontal lobe (F3) Before 20 15.2903 1.6958 2.5198 0.0208 During 20 13.7391 1.6858 Right frontal lobe (F4) Before 20 14.2286 1.5571 2.7245 0.0135 During 20 12.5327 1.4369 Left temporal lobe (T3) Before 20 10.3291 2.0557 1.1650 0.2584 During 20 8.1052 1.1702 right temporal lobe (T4) Before 20 7.9598 1.3750 1.7043 0.1046 During 20 6.1873 0.8721 Left parietal lobe (P3) Before 20 12.9352 1.6957 2.3293 0.0310 During 20 11.7075 1.7020 right parietal lobe (P4) Before 20 12.0750 1.4991 2.4131 0.0261 During 20 10.8800 1.3795

2. RST (Ratio of SMR to theta power spectrum)

Inhalation of flavor combination 1 increased the RST index of the experiment participants in the right frontal lobe (F4), right temporal lobe (T4), and left and right parietal lobes (P3, P4) (Table 8). It is the number divided by the wave area. SMR waves have a frequency band of 13 to 15 Hz, and are known to occur when attention is at its peak. The RST index is a ratio of theta waves to SMR waves representing attention and concentration, and is used as an EEG index representing attention ability. The fact that the RST increased with inhalation of the combination spice 1 indicates that the level of concentration increased.

Changes in RST index due to inhalation of blended spice 1 RST (ratio of SMR to theta power spectrum) brain location inhalation state number of subjects average Standard Deviation t-test p left prefrontal lobe
(Fp1) Before 20 0.3286 0.0413 -0.6610 0.5165 During 20 0.3558 0.0626 right prefrontal lobe
(Fp2) Before 20 0.3280 0.0449 -0.7597 0.4568 During 20 0.3676 0.0681 Left frontal lobe (F3) Before 20 0.3191 0.0388 -1.2825 0.2151 During 20 0.3552 0.0516 Right frontal lobe (F4) Before 20 0.2704 0.0321 -2.4764 0.0228 During 20 0.3355 0.0493 Left temporal lobe (T3) Before 20 0.5204 0.0594 -1.8174 0.0850 During 20 0.6084 0.0682 right temporal lobe (T4) Before 20 0.4419 0.0491 -3.0656 0.0064 During 20 0.6271 0.0916 Left parietal lobe (P3) Before 20 0.4931 0.0604 -3.4047 0.0030 During 20 0.6310 0.0860 right parietal lobe (P4) Before 20 0.4920 0.0724 -2.4808 0.0226 During 20 0.6186 0.0973

3. RSMT (Ratio of (SMR~mid beta) to Theta power spectrum)

The RSMT index of the experiment participants increased in the left temporal lobe (T3) by inhaling the combination flavor 1 (Table 9). The RSMT power spectrum is a neurophysiological index of EEG concentration with an SMR to mid beta/theta ratio. It has been reported that concentration increases when the SMR wave and mid beta wave increase while the theta wave decreases. Therefore, in this example, inhalation of spice combination 1 increases the RSMT power spectrum, so combination spice 1 is thought to have increased concentration.

Changes in RSMT index due to inhalation of mixed flavor 1 RSMT (Ratio of (SMR~mid beta) to theta) brain location inhalation state number of subjects average Standard Deviation t-test p left prefrontal lobe
(Fp1) Before 20 0.8859 0.1199 -0.0655 0.9484 During 20 0.8909 0.1444 right prefrontal lobe
(Fp2) Before 20 0.8961 0.1236 -0.0922 0.9275 During 20 0.9048 0.1532 Left frontal lobe (F3) Before 20 0.8425 0.0952 -0.7284 0.4752 During 20 0.8774 0.1027 Right frontal lobe (F4) Before 20 0.7367 0.0827 -1.1355 0.2703 During 20 0.7960 0.0943 Left temporal lobe (T3) Before 20 1.3727 0.1740 -2.1700 0.0429 During 20 1.6615 0.2066 right temporal lobe (T4) Before 20 1.3174 0.1751 -1.5392 0.1402 During 20 1.6938 0.3102 Left parietal lobe (P3) Before 20 1.1275 0.1200 -1.5716 0.1325 During 20 1.2675 0.1457 right parietal lobe (P4) Before 20 1.1047 0.1391 -1.5701 0.1329 During 20 1.2259 0.1523

4. Changes in ASEF index due to inhalation of blended fragrance 1

Inhalation of perfume combination 1 increased the ASEF (spectral edge frequency 50% of alpha spectrum band) index of the experiment participants in the left parietal lobe (P3) (Table 10). The ASEF index is the SEF50 of alpha waves, and it is known that it is possible to check the activity of alpha waves as an EEG index that represents comfort. The fact that the ASEF index was increased by inhalation of the combination flavor 1 indicates that the brain activity was increased.

Changes in ASEF index due to inhalation of blended fragrance 1 ASEF (spectral edge frequency 50% of alpha spectrum band) brain location inhalation state number of subjects average Standard Deviation t-test p left prefrontal lobe
(Fp1) Before 12 10.0156 0.1161 -1.5978 0.1266 During 12 10.1063 0.1162 right prefrontal lobe
(Fp2) Before 12 10.0219 0.1191 -1.3460 0.1942 During 12 10.1000 0.1165 Left frontal lobe (F3) Before 12 10.0000 0.1174 -1.1480 0.2652 During 12 10.0750 0.1110 Right frontal lobe (F4) Before 12 9.9969 0.1201 -0.9366 0.3607 During 12 10.0563 0.1143 Left frontal lobe (T3) Before 12 10.0375 0.1244 -1.8923 0.0738 During 12 10.1719 0.1193 right temporal lobe (T4) Before 12 10.0250 0.1126 -0.7368 0.4703 During 12 10.0719 0.1051 Left parietal lobe (P3) Before 12 10.0719 0.1219 -2.6164 0.0170 During 12 10.2188 0.1073 right parietal lobe (P4) Before 12 10.0781 0.1260 -1.5382 0.1405 During 12 10.1656 0.1122

Claims (3)

Aldehyde C14 1.2-1.8% by weight, Aldehyde C16 2.2-2.8% by weight, Benzyl acetate 3.5-4.5% by weight, Bergamot essential oil 12-14% by weight, Calone ) 2.7 ~ 3.3% by weight, cis-3-hexenol 7 ~ 8% by weight, citral 1.8 ~ 2.2% by weight, dipropylene glycol 14 ~ 16% by weight, he Hedion 6.5-7.5% by weight, Hydroxy citronellal 4.5-5.5% by weight, Lemon essential oil 4.5-5.5% by weight, Lilial 2.2-2.8% by weight, Linalool ( Linalool) 7-7.5% by weight, methyl anthranilate 1-1.5% by weight, phenyl ethyl alcohol 3.2-3.8% by weight, Sandalwood essential oil 4.5-5.5% by weight, tonalide (Tonalide) 6.5 ~ 7.5% by weight, Verdox (Verdox) 4.5 ~ 5.5% by weight, and a spice mixture containing 2.7 ~ 3.3% by weight of Korean fig tree extract as active ingredients, right frontal lobe, left frontal lobe, right frontal lobe, left AT (absolute theta power spectrum) was reduced in the parietal lobe and right parietal lobe, RST (Ratio of SMR to theta power spectrum) was increased in right frontal lobe, right temporal lobe, left parietal lobe and right parietal lobe, and RSMT (Ratio of ( A fragrance composition for increasing the comfort and concentration of the human brain, characterized by increasing SMR-mid beta) to Theta power spectrum) and increasing ASEF (spectral edge frequency 50% of alpha spectrum band) in the left parietal lobe. Based on the total weight of the fragrance mixture, Aldehyde C14 1.2 ~ 1.8% by weight, Aldehyde C16 2.2 ~ 2.8% by weight, Benzyl acetate 3.5 ~ 4.5% by weight, Bergamot essential oil 12 ~ 14 wt%, Calone 2.7-3.3 wt%, cis-3-Hexenol 7-8 wt%, Citral 1.8-2.2 wt%, Dipropylene glycol 14-16 wt%, Hedion 6.5-7.5 wt%, Hydroxy citronellal 4.5-5.5 wt%, Lemon essential oil 4.5-5.5 wt%, Lilial 2.2- 2.8 wt%, Linalool 7-7.5 wt%, Methyl anthranilate 1-1.5 wt%, Phenyl ethyl alcohol 3.2-3.8 wt%, Sandalwood essential oil 4.5 It is prepared by mixing ~5.5% by weight, Tonalide 6.5~7.5% by weight, Verdox 4.5~5.5% by weight and Korean fig tree extract 2.7~3.3% by weight, right frontal lobe, left frontal lobe, right frontal lobe, AT (absolute theta power spectrum) decreased in the left parietal lobe and right parietal lobe, RST (Ratio of SMR to theta power spectrum) was increased in the right frontal lobe, right temporal lobe, left parietal lobe and right parietal lobe, and RSMT (Ratio of (SMR ~ mid beta) to Theta power spectrum) and ASEF (spectral edge frequency 50% of alpha spectrum band) in the left parietal lobe, characterized in that the preparation of a fragrance mixture for increasing the comfort and concentration of the human brain method. A spice mixture for increasing the comfort and concentration of the human brain prepared by the method of claim 2.

Images