KR101714543B1 - Aroma composition for psycholigical stability and reducing stress comprising Schisandra chinensis essential oil and Angelica gigas essential oil as effective component - Google Patents

Abstract

The present invention relates to an aroma composition for psychological stability and stress reduction, wherein the aroma composition contains Schisandra chinensis essential oil and Angelica gigas essential oil as active ingredients. When a combined fragrant of Schisandra chinensis essential oil and Angelica gigas essential oil of the present invention is absorbed, an increase in an relative fast alpha power spectrum (RFA) and a spectral edge frequency 50% of alpha spectrum band (ASEF) is induced in the left lobus parietalis. An increase in a relative low beta power spectrum (RLB) and a ratio of SMR to theta (RST) is induced in the right lobus parietalis, and a decrease in a spectral edge frequency 90% (SEF90) is induced. Thus, psychological stability and stress reduction can be ensured.

Description

[0001] The present invention relates to a fragrance composition for psychological stability and stress relief comprising an essential oil of Schizandra chinensis essential oil and Angelica gigas essential oil as an active ingredient,

The present invention relates to a perfume composition for psychological stability and stress relief comprising an essential oil of Omija essential oil and true angelica essential oil as an active ingredient.

The cerebrum accounts for more than 75% of the total human brain volume, and is divided into left and right hemispheres. The cerebral cortex, which is the surface of the cerebral cortex, is a corrugated shape with a round protruding ridge and a fine ridge. Depending on the area, it is divided into frontal lobe, parietal lobe, temporal lobe, and occipital lobe. The cerebral cortex is known to have a specific function in each region. The frontal lobe is the largest among the leaves constituting the human brain, and is a region to control and control consciousness. It is a region of attention, memory, language, executive function, personality and emotion, , Reasoning and judgment, and decision making. The parietal lobe functions as a spatial perception function that measures the distance and position of the body by integrating sensory signals transmitted through the dorsal path of the spinal cord, and is involved in partial memory and attention. The occipital lobe is located in the visual cortex and is responsible for recognizing things.

In addition, the cerebral cortex plays a role in the human sense of sight, hearing, taste, and smell, especially the smell plays a role in identification of food, recognition of feeding, detection of danger, recognition of peers and parents (Mori Physiol Rev 86: 409-433) The process by which humans take the fragrance is that the odor molecules pass through the nasal passages first, and then to the olfactory nerves It reaches the epithelium, and the olfactory nerve cell in the olfactory epithelium senses it and transmits an electrical signal to the brain. The signal from the olfactory neuron is interpreted in the olfactory bulb and then recognized in the brain via two olfactory signal pathways, one pathway through the tonsil nucleus and the lateral hypothalamus to the cerebral cortex without passing through the thalamus, another Is a pathway through the olfactory cortex (the gourd cortex) through the thalamus to the cerebral cortex (Zetorre et al. (1992) Localization and lateralization of human olfactory cortex. Letters to Nature 360: 339-340) .

When the odorant molecules transmit the olfactory signal to the cerebral cortex, the nerve cells are constantly subjected to minute electrical activity, in which electric potential fluctuations occur on the scalp covering the surface of the cerebral cortex.

The electroencephalogram (EEG) is an electroencephalogram (EEG) that is obtained by attaching an electrode on the scalp and inputting electrical signals generated from the brain into the brain wave system to record the time potential. p. 1), EEG is able to record temporal and spatial changes of brain activity changing instantaneously, and it can measure brain function non-invasively, continuously, and it can record brain activity record, disease diagnosis, sleep study, (2010) Clinical Electroencephalography, Koryo Medicine, p. 1-77).

Recently, brain-computer interface (Brain-Computer Interface) has been used to read human's thoughts through brain waves and to adjust robot arm and robot chair.

Human brainwaves have amplitudes of 5 to 300 μV and frequencies between 0 and 60 Hz. Delta waves (0.5 to 3 Hz), theta waves (4 to 7 Hz), and alpha (8 ~ 13Hz), beta waves (14 ~ 30Hz), and gamma waves (30Hz and above). Delta waves and seta waves with a frequency lower than that of an alpha wave are referred to as slow waves, and beta waves and gamma waves with a frequency greater than that of an alpha wave are referred to as fast waves. Normally, the slow waves occur when the amplitude is high and the brain activity is decreased during the sleeping period, and the fast wave is mainly seen when the amplitude is low and the brain activity is promoted. Alpha wave is a fundamental wave, frequency is 8 ~ 13Hz, amplitude is 10 ~ 150μV and well recorded in parietal lobe and especially occipital lobe. A beta wave is a fast wave of 14-30 Hz with a low amplitude of 5-10 μV and appears when performing normal tasks or conscious actions and is activated when there is mental activity such as information processing. The gamma wave corresponds to the frequency range of 30 to 50 Hz and oscillates most rapidly among EEG waves. (Lee, 2011) Effects of Lime and Clove Fragments on EEG: An Analysis of the Effects of Lime and Clove on Brain Waves, Master Thesis, Kangwon National University, Seoul, Korea.

It is known that essence oil has a specific physical and chemical properties by gathering odor molecules. Most essential oils have a molecular weight of about 26-300 g / mol, a specific gravity of about 1.0, and the properties are known to be liquid phase and solid phase. Their properties are generally volatile, they are not soluble in water but are well soluble in alcohol and have a variety of functional groups.

Meanwhile, Schisandra chinensis ) is a ball with a diameter of about 1cm and a dark red color. It contains one to two reddish juice and reddish brown seeds. It has five flavors: sweet, sour, bitter, salty, and spicy. Omija contains ingredients such as iszindrin, gomilin, citral, malic acid, and citric acid, and strengthens the heart, lowers the blood pressure, increases the immunity, and is used as a tonic. It strengthens the pulmonary function, and it helps to cure the cough and thirst by the deep sea bream and the geodam function. I put the dried fruit in the cold water, and I put the honey and the sugar in the water which is red, and drink it with a drink, and it makes it to eat a leaf or a starch piece. Chestnuts, jujube, and mushrooms are put together and boiled to make tea or soak.

On the other hand, Angelica gigas grows near the stream valley. It is 1 ~ 2m in height and purple in the whole. The roots are large and fragrant, and the stems are straight. The leaves of the root and the bottom are 1-3 times haploid leaf. The small leaf is completely divided into 3 pieces and then divided into 2 to 3 again, with sharp sawtooth on the edge and white on the back. There is a small petiole on the small leaf of the tip. The leaf on the upper part is degenerated and the sheath becomes elliptical. Flower is bloomed from August to September and purple. Petal is 5 long oval, with pointed end and 5 stamens. There are 1 ~ 2 guns and it grows like a leaf sheath. The guns are thin like thread and 5 ~ 7. The fruit grows in October, is oval, has wings on the edge, and there is one oil pipe between the ridges. The young seeds are edible as herbs, the roots are called angelica, and they are used as medicines. Angelica sinensis (Angelica sinensis) from China, Angelica japonica, and Angelica japonica from Korea are called Angelica gigas. It is distributed in Korea, China, and Japan.

Korean Patent Laid-Open Publication No. 2003-0080107 discloses a technique for suppressing memory decline caused by stress and improving memory performance by a technique related to Omija or Chrysanthemum morifolium. Korean Patent Laid-Open Publication No. 2015-0028104 discloses a composition for preventing or treating a learning disorder or memory disorder containing an extract of Oriental japonica, Oriental japonica, 0804480 discloses a composition for improving memory performance. However, no perfume composition for psychological stability and stress relief has yet been disclosed which contains an essential oil of Omija essential oil and a true angelica essential oil as an active ingredient.

The present invention provides a perfume composition for psychological stability and stress relief comprising an essential oil component of Omija essential oil and Angelica gigas Nakai as an active ingredient, Inhalation of true Angelica japonica complex induces an increase in RFA (relative fast alpha power spectrum) and ASEF (50% of alpha spectrum band) in the left parietal lobe and RLB (Relative low beta power spectrum and RST (Ratio of SMR to theta) of the left prefrontal cortex and induce a reduction of SEF90 (90% of spectral edge frequency) in the left prefrontal cortex.

In order to achieve the above object, the present invention provides a psychological stability and a perfume composition for stress relief comprising an essential oil component of Omija essential oil and Angelica gigas Nakai as an active ingredient.

The present invention also provides a health functional food for psychological stability and stress relief comprising the perfume composition.

The present invention also provides a cosmetic composition for psychological stability and stress relief comprising the perfume composition.

The present invention relates to a perfume composition for psychological stability and stress relief comprising an essential oil of Omija essential oil and true angelica essential oil as an active ingredient. When the combined perfume of the present invention is inhaled, the RFA (Relative (RLB) and RST (Ratio of SMR to theta) in the right parietal lobe. In addition, In the left prefrontal cortex, the reduction of SEF90 (90% of the spectral edge frequency) is induced, and psychological stability and stress are alleviated. The composition of the present invention can provide not only flavor fragrance and food flavor raw materials but also to develop a stress-relieving neurofood which is added to foods to induce psychological stability.

[0035] Reference 1 is a photograph of the Omiza fruit sample (A) and the Angelica sp. Root sample (B) used in the present invention.
FIG. 2 is a photograph showing a method for attaching an electrode to a human brain for EEG measurement.

The present invention provides a perfume composition for psychological stability and stress relief containing an essential oil component of Omija essential oil and Angelica gigas Nakai as an active ingredient.

In the present invention, essential oil is a volatile organic compound extracted from leaves, stems, fruits, roots and flowers of plants by various methods. The essential oil is an essential oil obtained from a distillation extract of Omiza fruit, It is preferable that the essential oil extracted from the root extract of Angelica gigas Nakai is supercritical carbon dioxide, and the essential oil extracted by other methods is incompatible because of different flavor.

The mixing ratio of the Omija essential oil and the true Angelica essential oil complex is preferably 10: 8: 1, more preferably 10: 1, but is not limited thereto.

The composition induces an increase in RFA (relative fast alpha power spectrum) and ASEF (50% of alpha spectrum band) in the left parietal lobe and a relative low beta power spectrum (RLB) and Ratio of SMR to theta, and induces a decrease in SEF90 (90%) in the left prefrontal cortex. The increase in RFA and ASEF is attributed to an increase in fast alpha wave ratio . As the rapid increase in ALPA increases the concentration of the brain by awakening the brain, it is in a state of being able to feel stability and comfort.

The increase in RLB means that the ratio of low beta waves is increased in the entire EEG, and the increase in the low beta wave rate causes the brain to awaken and make the head clear and cool. The increase in RST means that the ratio of theta wave in the SMR wave is increased, which means that the attention ability is increased.

A decrease in SEF90 means that the level of mental stress and stress has been mitigated.

The perfume composition may include cosmetics including perfumes, soap cleansing products including bath soaps, indoor cleaning products including glass cleaners, aroma products including car perfumes, bath products including herbal bath salts, stationery Such as perfumes, fragrances, perfumes for offices, perfumes for office use, or industrial products containing synthetic resins.

The formulation of the perfume composition is not particularly limited, but may be any one selected from powders, granules, liquid spray, solid and gel type formulations.

The present invention also provides a health functional food for psychological stability and stress relief comprising the perfume composition.

The health functional foods include various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, colorants and enhancers (cheese, chocolate etc.), pectic acid and its salts, alkynic acid and its salts, , pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. It may also contain flesh for the production of natural fruit juices and vegetable drinks. These components may be used independently or in combination.

The health functional food of the present invention may contain various flavors or natural carbohydrates as an additional ingredient. The natural carbohydrates are sugar saccharides such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like.

The present invention also provides a cosmetic composition for psychological stability and stress relief comprising the perfume composition.

The cosmetic composition of the present invention can be used for cosmetics such as softening lotion, convergent lotion, nutritional lotion, nutrition lotion, nutrition essence, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, , A body lotion, a body cream, a body oil, a body essence, a lotion, an ointment, a gel, a cream, a patch, and a spray, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

Example  1. Acquisition and storage of plant samples

The omija and chrysanthemum angustifolia used in the present invention were purchased from Heilong Nature Agriculture Company Incorporated, 340-50 Songjeong-gil, Jinbu-myeon, Pyeongchang-gun, Gangwon-do. The purchased omija was dried fruit, and Angelica angustifolia was a piece of dried root (Fig. 1).

When purchasing Omiza and Chrysanthemums used in this example, they received a Certificate of Origin that they were cultivated in Korea from the corporation of Healing Necture Agriculture Company. They were stored in a refrigerator at 4 ℃ immediately after they arrived at the laboratory to suppress the volatilization of volatile fragrance materials. And was taken out immediately before the experiment.

Example  2. Extraction of natural essential oils

1) Extraction of Omija essential oil

Omija was ground using a hood mixer (DA280-2, DAESUNG ARTLON, Korea). In this Example, Omiza obtained by pulverizing Omiza berries in an instant cut mode 6 times for 5 seconds was used. Extraction of the essential oil from the ground oyster was carried out using a steam distillation apparatus (EssenLab-Plus, HANIL LABTECH, Korea).

Two liters of distilled water was placed in the water vapor generating portion of the steam distillation apparatus, and 1 kg of omija was placed in the region where the essential oil was generated. Then, cooling water of 4 ° C was continuously flowed in the cooling tube. The water vapor was generated while maintaining the temperature of the steam generation site at 100 ° C for 90 minutes, and the essential oil contained in the ozone condensed in the cooling tube and collected in the distillation water layer and the refinery layer in the receiver. In order to collect only pure essential oil, the essential oil layer was passed through a triangular funnel containing sodium sulfate anhydrate to remove moisture and impurities. The collected essential oil was stored in a refrigerator at 4 ° C until analysis of fragrance, component analysis, and EEG analysis.

The recovery rate of essential oil was 0.57% and the standard deviation was 0.04%.

Recovery of Essential Oil Extracted from Omija Seed Samples
sample Recovery rate (%)
Average (%)
Standard Deviation (%) Repeat 1 Repeat 2 Repeat 3 Dried omija seeds 0.64 0.58 0.50 0.57 0.04

2) Extraction of Chrysanthemum essential oil

The crude oil was extracted using a supercritical carbon dioxide extraction system (ISA-SCCO-S-050-500, ILSHIN, Korea). 100 g of Angelica gigas Nakai was placed in the extraction tank and the essential oil was extracted at 400 bar and 50 ° C, respectively, under the pressure and temperature of liquid carbon dioxide.

In this Example 2, the extraction pressure and the temperature of the Angelica gigas are according to the condition that showed the highest recovery rate through the previous study (pressure, 200, 300, 400 bar; temperature 40, 50, 60 ° C). At the optimum temperature and pressure condition, supercritical carbon dioxide was extracted for 1 hour and the essential oil was extracted. The extracted essential oil was passed through a triangular funnel containing sodium sulfate anhydrate to remove moisture and impurities. The collected essential oils were stored in a refrigerator at 4 ° C until the analysis of the fragrance, component analysis, and EEG analysis.

The recovery rate of essential oils obtained from the root samples of Angelica gigas Nakai using supercritical carbon dioxide extraction system was 3.01% and the standard deviation was 0.27%.

Recovery rate of essential oil extracted from Angelica gigas Root samples
sample Recovery rate (%)
Average (%)
Standard Deviation (%) Repeat 1 Repeat 2 Repeat 3 Root of Angelica gigas 3.56 2.71 2.77 3.01 0.24

Example  3. Odor Evaluation of Natural Essential Oils

The aroma evaluation of the Omija and Angelica japonica essential oils obtained in Example 2 above was carried out by five expert panels of Rose of Sharon, a perfume development club member of Kangwon University, which has been training for more than six months. (100%) of Omija and Angelica japonica extract extracted from Example 2 were wetted on a shade paper (0.5 cm × 5 cm), and then the shade paper was placed at a position of 5 cm from the nose of the panel. And the fragrance evaluation term (Table 3). The flavor types used in the sensory evaluation were aldehyde, animalic, balsamic, citrus, coniferous, earthy, floral, fruity, ), Green, herbal, medicinal, minty, marine, mossy, musky, oily, oriental, Powdery, smoky, spicy, sweet, and woody, typically used by a group of flavor assessment experts.

Flavor types and properties of essential oils Drape type characteristic example Aldehyde Incense like fat or fat Aldehyde C9 Animalic Incense with animal odor Civet cat oil Balsamic Sweet and warm feeling like normal vanilla Benzoin Citrus Fresh citrus flavor, sweet and refreshing feeling Bergamot Coniferous Similar to fresh, clean pine scent Cajuput Earthy The unique aroma of the atmosphere when it rains Vetiver Floral The fragrance of a single flower The fragrance of a bouquet Jasmin, Rose Fruity Peaches, strawberries, and tropical fruits Apple, Plum Green Flavor when cutting or rubbing leaves Galbanum Herbal A fragrant and stimulating plant like wormwood Lavender Medicinal An incense reminiscent of medicine Anis Minty Menthol flavor of peppermint or spearmint Peppermint Marine A slightly salty incense reminiscent of a cool ocean Calone Musky Fragrant incense Musk Oily Fragrant aroma like olive oil Olive Oriental A mysterious fragrance that is widely supplied from the Orient Opopanax Powdery Fragrance Heliotropin Smoky I smell smoke from smoked fish Spicy Incense of stimulating feeling from spice Clove Sweet Sweet, fragrant and pleasurable Ethyl maltol Woody A gentle and profound tree fragrance from trees Sandalwood

The aroma of Omija essential oil extracted using the steam distiller in this Example 3 can be obtained from animalic, balsamic, citrus, earthy, floral, fruity, green, medicinal, oily, sweet, and woody (Table 4).

In addition, the fragrance of the true Angelica essential oil extracted using the supercritical carbon dioxide extractor can be obtained from balsamic, earthy, herbal, medicinal, oriental, spicy, sweet) (Table 4).

Flavor types and properties of essential oils Drape type Omiza spices Chrysanthemum sp. Aldehyde Animalic ● Balsamic ● ● Citrus ● Coniferous Earthy ● ● Floral ● Fruity ● Green ● Herbal ● Medicinal ● ● Minty Marine Musky Oily ● Oriental ● Powdery Smoky Spicy ● Sweet ● ● Woody ●

Example  4. Development and Preference of Combination Spices Using Natural Essential Oil

In Example 2, three kinds of new combination fragrances were developed by combining the essential oil extracted with steam distillation and the essential oil extracted with supercritical carbon dioxide (Table 5). The omija essential oil and the true angelic essential oil have their own unique fragrance and have a distinctive flavor completely different from that of the omija and the true angelic essential oil when mixed.

10 of Rose of Sharon Expert Panel participated in Kangwon University Perfume Development Circle which had at least 6 months olfactory training for preference survey on three kinds of combined fragrance. Each of the combined fragrance stock solutions (100%) was soaked in a tissue paper (0.5 cm × 5 cm), and then placed in a position 5 cm from the nose of the panel to position the scent paper. The perfume was scored 10 points on the most preferred fragrance, Points were given. Table 5 shows the average values based on the preference results.

Consumer preference for Omija essential oil was 5.0, and consumer preference for true Angelica essential oil was 5.7. Based on consumer preference results for Omija refined oil and true angel oil refined oil, it was judged that the two kinds of natural refined oil will be acceptable to consumers.

Consumer preference scores of the combination spices 1, 2, and 3, which consisted of the combination of Omija essential oil and Chrysanthemum essential oil, were 4.6, 5.0, and 5.5, respectively.

Composition and Composition for New Spice Development Composition Composition ratio (weight ratio) Consumer preference
(medium) Omija oil True Angelica Oil Omija oil One 0 5.0 True Angelica Oil 0 One 5.7 Combination Spices 1 One One 4.6 Combination Spices 2 5 One 5.0 Combination Spices 3 10 One 5.5

Example  5. Effect of Combination Fragrance 3 on EEG

A diluted solution (hereinafter referred to as 'combined fragrance 3') diluted to 2% by adding anhydrous ethanol to the combined fragrance 3 of Example 4 was prepared.

In Example 5, brain waves were measured in order to know how the inhalation of the combined fragrance 3 affected the human brain. EEG measurements were performed on 12 subjects (6 men and 6 women) in a 32.4 - square - meter laboratory, where temperature and humidity were maintained at 23 ℃ and 50% humidity, respectively.

The EEG measurement device used for EEG measurement was QEEG-8 System (LXE3208, LAXTHA Inc. Korea) and EEG signals were measured using LAXTHA data acquisition equipment and analysis program (Telescan). Electro-Cap Internationals, Inc., USA) was used for electroencephalography (EEG), and the left and right frontal lobes (Fp1, Fp2), left and right frontal lobes (Fig. 2) were attached to the left and right temporal lobes (T3 and T4), left and right parietal lobes (P3 and P4), and one electrode (Covidien ^TM ) .

In a paper cup of 5 cm in height, 4.8 cm in height, and 3.5 cm in diameter on the top, gauze of 3.5 cm in length and 4 cm in length was placed and added to the gauze using a pipette to maintain the distance from the subject's nose to about 5 cm. The eyes were closed for 45 seconds before incense inhalation (irritation) and inhalation (incense irritation), respectively. In the data processing obtained through EEG measurement for the diluted solution of the combined fragrance 3 in the fifth embodiment, only a representative 30-second electroencephalogram signal among the EEG signals of the test subject was extracted and subjected to an EEG batch processing, A corresponding sample T test was performed using the SPSS program (SPSS 18.0 version).

Table 6 shows the types of EEG indicators for data analysis obtained before inhalation (unstimulated) and during inhalation (incense stimulation) of Combination Fragrance 3 in Example 5, and the meanings of the EEG indices are shown in Table 7 .

Types of EEG indicators used in data analysis No. Analysis index Full name of EEG indicator Waveband (Hz) One AT Absolute Theta Power Spectrum 4 to 8 2 AA Absolute Alpha Power Spectrum 8-13 3 AB Absolute Beta Power Spectrum 13 to 30 4 AG Absolute Gamma Power Spectrum 30 to 50 5 AFA Absolute Fast Alpha Power Spectrum 11-13 6 ASA Absolute Slow Alpha Power Spectrum 8 ~ 11 7 ALB Absolute Low Beta Power Spectrum 12 to 15 8 AMB Absolute Mid Beta Power Spectrum 15-20 9 AHB Absolute High Beta Power Spectrum 20 ~ 30 10 RT Relative Theta Power Spectrum (4 to 8) / (4 to 50) 11 RA Relative Alpha Power Spectrum (8 to 13) / (4 to 50) 12 RB Relative Beta Power Spectrum (13 to 30) / (4 to 50) 13 RG Relative Gamma Power Spectrum (30 to 50) / (4 to 50) 14 RFA Relative Fast Alpha Power Spectrum (11 to 13) / (4 to 50) 15 RSA Relative Slow Alpha Power Spectrum (8 to 11) / (4 to 50) 16 RLB Relative Low Beta Power Spectrum (12 to 15) / (4 to 50) 17 RMB Relative Mid Beta Power Spectrum (15 to 20) / (4 to 50) 18 RHB Relative High Beta Power Spectrum (20 to 30) / (4 to 50) 19 RST Ratio of SMR to Theta (12 to 15) / (4 to 8) 20 RMT (Ratio of Mid Beta to Theta) (15 to 20) / (4 to 8) 21 RSMT Ratio of (SMR ~ Mid Beta) to Theta (12 to 20) / (4 to 8) 22 RAHB Ratio of Alpha to High Beta (8 to 13) / (20 to 30) 23 SEF50 Spectral Edge Frequency 50% 4 to 50 24 SEF 90 Spectral Edge Frequency 90% 4 to 50 25 ASEF Spectral Edge Frequency 50% of Alpha Spectrum Band 8-13

Meaning of EEG indices used in data analysis No. Analysis index meaning One AT Absolute power of theta wave (meditation, sleepy) 2 AA Absolute power of Alpha wave (relaxation, stability) 3 AB The absolute power of Beta waves (tension, arousal, activity, cognitive function) 4 AG Absolute power of the Gamma wave (highly cognitive or anxious, excited) 5 AFA Fast alpha Wave's absolute power (relaxed, immersive, creativity) 6 ASA Absolute power of Slow alpha wave (relaxation) 7 ALB Absolute power of the low beta wave, SMR parameter (caution or boundary) 8 AMB Mid beta wave absolute power (concentration, attention) 9 AHB High beta Absolute power (stress, mental load, tension) 10 RT Percentage of theta waves in total 11 RA The ratio of alpha waves in total 12 RB Ratio of beta waves in total 13 RG The ratio of gamma waves in total 14 RFA The ratio of fast alpha waves in total 15 RSA The ratio of slow alpha wave in total 16 RLB The ratio of low beta waves in total 17 RMB The ratio of mid beta waves in total 18 RHB The ratio of high beta waves in total 19 RST The ratio of theta wave in SMR wave 20 RMT The ratio of theta wave in mid beta waves 21 RSMT SMR ~ mid beta wave / theta wave (EEG concentration indicator) 22 RAHB Alpha wave / high beta wave (stable and relaxed) 23 SEF50 Median Frequency Brain activity (EEG) 24 SEF 90 Median Frequency Brain activity (mental stress, stress) 25 ASEF Alpha wave SEF50 (comfort: fast alpha waves)

In the fifth embodiment, among the 25 EEG analysis indexes, a relative fast alpha power spectrum (RFA), a relative low beta power spectrum (RLB), a Ratio of SMR to theta, a spectral edge frequency 90% There was a statistically significant difference (p <0.05) between the unstimulated and incongruent stimuli only at the edge frequency of 50% of the alpha spectrum band.

1) Relative fast alpha power spectrum (RFA)

The RFA indices of subjects with statistical significance increased from 0.1629 to 0.1914 in the left parietal lobe (P3) with the inhalation of combination fragrance 3 (Table 8).

The RFA index is a ratio of the absolute power of each frequency based on the absolute power of the entire frequency band in the EEG spectrum. It is a spectrum value obtained by dividing the fast ALPHA region (11 ~ 13Hz) into the entire frequency region (4 ~ 50Hz) .

The increase in the RFA index in Example 5 indicates that the combination perfume 3 induces psychological stability by making the brain feel more comfortable while concentrating and concentrating the brain.

RFA Indicator Changes due to Inhalation of Combination Spice 3 Relative fast alpha power spectrum (RFA) Site inhale N Mean SD t-test p Fp1 Before 12 0.0908 0.08997 -0.6846 0.5077 During 12 0.0953 0.08332 Fp2 Before 12 0.0881 0.08989 -0.8203 0.4294 During 12 0.0943 0.08120 F3 Before 12 0.0957 0.09989 -1.3686 0.1984 During 12 0.1059 0.09557 F4 Before 12 0.0921 0.09431 -1.1832 0.2616 During 12 0.1023 0.08835 T3 Before 12 0.1137 0.08304 -0.0003 0.9997 During 12 0.1137 0.07328 T4 Before 12 0.0841 0.06596 0.1982 0.8464 During 12 0.0829 0.05318 P3 Before 12 0.1629 0.15292 -2.2985 0.0421 During 12 0.1914 0.15859 P4 Before 12 0.1532 0.15796 -2.1177 0.0577 During 12 0.1788 0.15495

Fp1: left prefrontal, Fp2: right prefrontal, F3: left frontal, F4: right frontal, T3: left tomporal, T4: right temporal Right temporal), P3: left parietal, P4: right parietal,

2) Relative low beta power spectrum (RLB) index change due to inhalation of combination fragrance 3

Inhalation of the combined fragrance 3 increased the RLB indices in the right parietal lobe (P4) of the subjects (Table 9).

The RLB index is an absolute power ratio of the low beta band to the absolute power of the entire frequency region in the EEG spectrum and is defined as the ratio of the low beta waveband (12 to 15 Hz) to the entire frequency band (4 to 50 Hz) With the spectral value divided, the increase in the RLB index means that the combination perfume 3 of the present invention awakens the brain.

RLB Indicator Changes due to Inhalation of Combination Spice 3 Relative low beta power spectrum (RLB) Site Inhalation N Mean SD t-test p Fp1 Before 12 0.0545 0.02159 -0.7876 0.4475 During 12 0.0606 0.03335 Fp2 Before 12 0.0526 0.02296 -0.6051 0.5573 During 12 0.0575 0.03300 F3 Before 12 0.0565 0.02514 -0.7414 0.4739 During 12 0.0628 0.03490 F4 Before 12 0.0550 0.03097 -0.4453 0.6647 During 12 0.0592 0.03627 T3 Before 12 0.0815 0.02906 0.4502 0.6612 During 12 0.0788 0.02891 T4 Before 12 0.0677 0.03362 0.5502 0.5931 During 12 0.0635 0.02837 P3 Before 12 0.0842 0.03640 -1.1979 0.2561 During 12 0.1054 0.06203 P4 Before 12 0.0823 0.05924 -2.2067 0.0494 During 12 0.1104 0.08000

Fp1: left prefrontal, Fp2: right prefrontal, F3: left frontal, F4: right frontal, T3: left tomporal, T4: right temporal Right temporal), P3: left parietal, P4: right parietal,

3) Ratio of SMR to theta due to inhalation of combination fragrance 3

The RST index increased from 0.5873 to 0.9416 in the right parietal lobe of subjects (P4) by inhalation of the combined fragrance 3 and was found to be statistically significant (Table 10).

RST is the spectral value obtained by dividing SMR wave region (12 ~ 15Hz) by theta wave region (4 ~ 8Hz) at the ratio of SMR / theta in the EEG spectrum, It is an analysis index. The increase in the RST index in Example 5 means that the attention concentrating ability of the combination perfume 3 is increased by inhalation.

RST index change due to inhalation of combination fragrance 3 Ratio of SMR to theta (RST) Site Inhalation N Mean SD t-test p Fp1 Before 12 0.3162 0.21384 -0.4824 0.6389 During 12 0.3376 0.19314 Fp2 Before 12 0.3224 0.22566 -0.5272 0.6085 During 12 0.3420 0.21174 F3 Before 12 0.2954 0.16558 -1.3155 0.2150 During 12 0.3616 0.26152 F4 Before 12 0.2823 0.17131 -1.3554 0.2024 During 12 0.3612 0.27772 T3 Before 12 0.5076 0.32370 0.4971 0.6288 During 12 0.4821 0.29545 T4 Before 12 0.6185 0.46715 0.3530 0.7307 During 12 0.5780 0.33643 P3 Before 12 0.5823 0.31045 -1.3787 0.1953 During 12 0.8095 0.72890 P4 Before 12 0.5873 0.44982 -2.4822 0.0304 During 12 0.9416 0.74593

Fp1: left prefrontal, Fp2: right prefrontal, F3: left frontal, F4: right frontal, T3: left tomporal, T4: right temporal Right temporal), P3: left parietal, P4: right parietal,

4) SEF90 (spectral edge frequency 90%) due to inhalation of the combination fragrance 3

Inhalation of the combined fragrance 3 reduced the SEF90 (spectral edge frequency 90%) indicator of the subjects in the left prefrontal cortex (Fp1) (Table 11). SEF 90, which is a general indicator of brain stress, shows the cognitive load felt during the task, namely, mental stress level and excessive mental arousal level. The decrease in the SEF90 index in Example 5 indicates that Combination Spice 3 mitigates the stress level And the subjects were psychologically stable.

SEF90 indicator change due to inhalation of combination fragrance 3 SEF90 (Spectral edge frequency 90%) Site Inhalation N Mean SD t-test p Fp1 Before 12 27.6458 8.67731 2.2516 0.0457 During 12 25.1563 9.15284 Fp2 Before 12 27.1615 8.96432 2.0566 0.0642 During 12 24.6719 9.46431 F3 Before 12 23.7917 7.69206 1.5875 0.1407 During 12 21.8281 6.54633 F4 Before 12 22.6875 8.18960 1.6939 0.1183 During 12 20.4167 6.79197 T3 Before 12 28.4375 7.42166 0.0093 0.9927 During 12 28.4219 7.35920 T4 Before 12 29.5833 7.95193 -0.5783 0.5746 During 12 30.4583 9.42812 P3 Before 12 23.1250 7.43891 1.0240 0.3278 During 12 22.0833 5.67054 P4 Before 12 22.8438 7.12024 1.4089 0.1864 During 12 21.3802 5.69679

Fp1: left prefrontal, Fp2: right prefrontal, F3: left frontal, F4: right frontal, T3: left tomporal, T4: right temporal Right temporal), P3: left parietal, P4: right parietal,

5) ASEF (spectral edge frequency 50% of alpha spectrum band) change due to inhalation of combination fragrance 3

In this Example 5, the ASEF index of subjects was statistically significantly increased in the left parietal lobe (P3) by inhalation of the combined fragrance 3 (Table 12). The ASEF index is an EEG index showing comfort with SEP 50 of ALPHA, which means relaxation of the body and mind. In this Example 5, the combination perfume 3 increases the ASEF index, which means that the mind and body are stabilized.

ASEF indicator changes due to inhalation of combination fragrance 3 ASEF (Spectral edge frequency 50% of alpha spectrum band) Site Inhalation N Mean SD t-test p Fp1 Before 12 9.9688 0.71286 -0.7647 0.4605 During 12 10.0365 0.59080 Fp2 Before 12 9.9010 0.74927 -1.1721 0.2658 During 12 10.0208 0.58225 F3 Before 12 9.9635 0.71681 -1.0218 0.3288 During 12 10.0625 0.61527 F4 Before 12 9.8698 0.77398 -0.7101 0.4924 During 12 9.9635 0.54711 T3 Before 12 10.1927 0.73973 -0.8554 0.4105 During 12 10.2760 0.64538 T4 Before 12 9.9948 0.77901 -1.0586 0.3124 During 12 10.1354 0.52955 P3 Before 12 10.2552 0.62753 -2.9223 0.0138 During 12 10.5365 0.64593 P4 Before 12 10.0573 0.79391 -2.2364 0.4605 During 12 10.3906 0.77015

Fp1: left prefrontal, Fp2: right prefrontal, F3: left frontal, F4: right frontal, T3: left tomporal, T4: right temporal Right temporal), P3: left parietal, P4: right parietal,

Claims (9)

The mixture of Omija essential oil and Angelica japonica essential oil obtained by mixing Omija essential oil and true Angelica japonica extract obtained from Omija fruit distillation extract at a weight ratio of 10 to 8: 1 was used as an active ingredient, and the left parietal lobe , The relative fast alpha power spectrum (RFA) and the ASEF (spectral edge frequency 50% of the alpha spectrum band) were induced in the right parietal lobe and the RLB (relative low beta power spectrum) and RST (Ratio of SMR to theta) And induces a decrease in SEF90 (90%) in the left prefrontal cortex. delete delete delete The perfume composition according to claim 1, wherein the formulation of the perfume composition is any one of powder, granule, liquid spray, solid and gel type. A health functional food comprising the perfume composition according to claim 1. [7] The composition according to claim 6, further comprising at least one selected from the group consisting of vitamins, minerals, flavors, colorants, pectic acid and salts thereof, alkynic acids and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, , An alcohol, and a carbonating agent used in a carbonated beverage. A cosmetic composition comprising the perfume composition according to claim 1. The cosmetic composition according to claim 8, wherein the cosmetic composition is at least one selected from the group consisting of softening agents, convergent lotion, nutritional lotion, nutritional lotion, nutritional essence, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, Wherein the composition is selected from the group consisting of a gel, a powder, a makeup base, a body lotion, a body cream, a body oil, a body essence, a lotion, an ointment, a gel, a cream, a patch and a spray.

Images