KR102000095B1 - Aroma composition enhances psychological stability of human by increasing the relative fast alpha activity at T8 region and decreasing the relative gamma activity at Cp6 region and uses thereof - Google Patents

Abstract

The present invention relates to a combined perfume composition of the herbal essence type which not only increases the RFA index at the temporal lobe T8 region but also reduces the RG index at the Cp6 region to induce mental and physical stability, chamomile spices, caraway spices, lemon balm spices, silybum marianum spices, angelica spices, ethyl alcohol, d-limonene, A herbal extract composition comprising peppermint essential oil extract, mandarin orange essential oil extract, elder flower absolute, vanilla bean absolute, and geranium oil egypt. (RFA) at the T8 region of the temporal lobe in the analysis of brain waves. In addition, the RG index is decreased at the Cp6 region to relax and stabilize the mind and body. Key has the property. The combined perfume composition of the present invention can be applied to the development of a fragrance product used for the purpose of relaxation and stability of mind and body during use.

Description

[0002] The present invention relates to a herbal essence combination perfume composition which increases the RFA index in the temporal lobe T8 region as well as reduces the RG index in the Cp6 region to induce the stability of mind and body. the relative gamma activity at Cp6 region and uses thereof}

The present invention relates to a combined perfume composition of the herbal essence type which not only increases the RFA index at the temporal lobe T8 region but also reduces the RG index at the Cp6 region to induce mental and physical stability, chamomile spices, caraway spices, lemon balm spices, silybum marianum spices, angelica spices, ethyl alcohol, d-limonene, A herbal extract composition comprising peppermint essential oil extract, mandarin orange essential oil extract, elder flower absolute, vanilla bean absolute, and geranium oil egypt. (HbA1c) in the temporal lobe of the T8 region. In addition, the RFA index is increased in the T8 region of the temporal lobe and the RG index is decreased in the Cp6 region to relax the mental and physical stability It has the property to purify.

Perfume is a complex of volatile organic compounds, which are fragrance-transmitting substances. Most of them have a molecular weight of 26 to 300 g / mol. Specific gravity is around 1.0, and its properties are liquid, dendritic, or crystalline and volatile. Has good melting properties. Spices can be classified according to the material, natural fragrance can be obtained from natural and synthetic fragrance can be divided into organic synthesis.

Natural spices are divided into animal spices, which can be obtained from animals, and vegetable spices, which can be obtained from plants. Animal spices include ambergris from sperm whales, civetone from musk cat, muskone from musk deer ), And a castorium obtained from a beaver. Vegetable spices can be collected from about 1,500 plants worldwide. Representative vegetable spices include bergamot, clary sage, eucalyptus, grapefruit, jasmine, juniper, lavender, lemon, orange, patchouli, peppermint, rose, sandalwood, tea tree , vanilla, ylang-ylang, benzoin, cinnamon bark, atlas cedarwood, cypress, hyssop, mandarin pine, roman chamomile, thyme and yarrow. Plant spices, which are easier to harvest than animal spices, are now being sold in the market.

Methods for obtaining spices from plants depend on the type of plant and the extraction site, and steam distillation, compression, volatile solvent extraction, and nonvolatile solvent extraction using supercritical carbon dioxide are used. The advantages and disadvantages of each extraction method are shown in Table 1 below.

Comparison of strengths and weaknesses between fragrance extraction methods Extraction method Advantages Disadvantages Steam distillation method o The most economical way.
o Large amounts can be extracted in a short time  o Fragrance materials may be decomposed by water and heat. Compression method  o It is not affected by heat.  o Extracts only citrus oils Volatile solvent extraction method  o Relatively high yield  o Completely remove volatile solvents Nonvolatile solvent extraction method  o No need to remove solvent o Need a lot of time
o Need a lot of labor

Spices are volatile organic compounds, and when they enter the human nasal cavity during volatilization they are known to cause electrical signals through binding to the nasal olfactory receptors. The electrical and chemical signals generated in the brain can be obtained through computed tomography (CT), magnetoencephalography (MEG), functional magnetic resonance image (fMRI), and electroencephalography (EEG). EEGs measuring EEG are noninvasive and real- There is a point of getting data with.

Electroencephalogram (EEG) is an external measurement of the electrical changes caused by the activity of the brain neurons. At this time, since the potential fluctuation of the EEG measured on the scalp has a very low potential of several hundred μV from several μV, the electric potential obtained by bonding the electrode on the scalp is amplified through the brain wave system. The electroencephalogram (EEG) is called the electroencephalogram (EEG), which is derived from the electric potential generated in the scalp by the electrode and is input to and amplified in the brain wave system. ). EEG can be classified into various types such as electrocorticogram (ECoG), sphenoidal electrode EEG, foramen ovale electrode EEG, depth electrode EEG, Has been developed and used for simple spasmodic diseases.

The brain waves that occur in the human brain include delta waves at frequencies of 0.5 to 3 Hz, theta waves at 4 to 7 Hz, alpha waves at 8 to 13 Hz, beta waves at 14 to 30 Hz, and gamma waves at frequencies above 30 Hz , the delta wave is a slow wave with a frequency of 0.5 to 3 Hz. Especially, when it is in deep sleep, it is detected much in the frontal part. Theta wave shows frequency of 4 ~ 7Hz, It occurs when a state of super-learning ability such as an accident occurs.

The Alpha wave has a frequency of 8 ~ 13Hz and an amplitude of 10 ~ 150μV. It is well recorded in the parietal and occipital lobe and occurs in aroused and stable state of normal adult. The alpha waves are divided into slow alpha waves (7-8Hz), mid alpha waves (9-11Hz) and fast alpha waves (12-13Hz). The slow alpha waves are relaxed, It occurs in a concentrated state. The Mid alpha wave is characterized by normal accidents and does not involve tension, and appears when meditation, creative activity, or efficient injection of new information or knowledge into the brain, The effect of lime flavor and clove flavor on brainwaves. (Thesis of Master's Thesis, Kangwon National University).

Beta waves are fast waves with frequencies between 14 and 30 Hz and low amplitudes of between 5 and 10 μV. They are activated when there is mental activity, such as information processing, that occurs when doing normal work or conscious behavior. Beta waves are classified into slow beta waves (12-15 Hz), mid beta waves (15-20 Hz) and high beta waves (20-30 Hz) depending on the frequency region. Slow beta waves are sensory motor rhythm , And attention is paid to minimize the activity of the motor cortex without motion. The mid beta wave appears when performing an accident activity such as focusing on a specific subject. In addition, high beta waves are more likely to be involved in complex information processing activities, such as relatively complex reasoning or high mental load, or in emotional tension, anxiety, and excitement (Lee, YH, Kim, EEG Recall Recreation Journal 31 (3): 101-122). When you are in normal tension with proper tension, beta waves usually occur and work effectively, but if you are stressed and over-excited by tension, you will get high beta waves.

(Heracleum moellendorffii) natural essential oil showed mental concentration and arousal effect through the decrease of slow alpha wave and the increase of high beta wave in EEG in the aquarium (a flour composition containing aquatic essential oil extracted from a leaf of aquarium) 2013. A flame-retardant composition comprising an aquatic natural essential oil extracted from an aquatic leaf. Korean Patent No. 10-1313255). Kim et al. (2005) used indene, 3-methylstyrene, beta-styrene, beta- 10% of hydroxy citronellal (15%), 8% of PEA (15%), 6% of benzoin (5%), and benzyl acetate (10%) were added to the leaves of Mugunghwa leaf which was composed of 13 kinds of volatile organic compounds such as cyanophyllene, dureen, Sweet, floral, soft, powdery, warm, pink with 6%, lyral (5%) and 6% terpineol (10% (Korean Patent No. 10-1252552). (Combination perfume composition that reduces beta-wave in left prefrontal (Fp1) region and decreases SEF50 (50% in spectral edge frequency) in the right prefrontal (Fp2) But are not limited to, oil, angelica oil, patchouli oil, sandalwood oil, galaxolide, ligustral, linalool, linalyl acetate, But are not limited to, lilial, vanillin, styrallyl acetate, cis-3-hexenyl salicylate, cinnamic aldehyde, allyl amyl glycolate, amyl glycolate, ethyl maltol, canthoxal, tonalide, and hedion is a relatively fragile composition of the left frontotemporal region in which human cognitive functions are highly concentrated during brain wave analysis It has been reported that not only the relative beta power is significantly decreased but also the SEF50 value of the right prefrontal region is significantly decreased to relax and stabilize the body and mind (Korean Patent No. 10-134952). (The absolute low beta power spectrum of the left prefrontal region Fp1 and the absolute high beta power spectrum of the right parietal region P4 and also the left parietal region A perfume composition having the property of enhancing the concentration of attention by increasing the RMT index (ratio of the middle beta wave to theta wave) in the right parietal region (P3) and the right parietal region (P4) But are not limited to, lemon oil, basil oil, bergamot oil, vetiver oil, acorus oil, patchouli oil, petitgrain oil, methylionone gamma but are not limited to, methyl ionone gamma, benzyl acetate, citral, evernyl, eugenol, coumarin, hedion, hexyl cinnamic aldehyde ), Helianal (helional) combination perfume consists citrus (cit rus, coniferous, green, herbal, minty, oily, powdery, and spicy scent. In addition, EEG analysis of combined perfume showed statistically significant decrease in the absolute low beta power spectrum of the left prefrontal region (Fp1) and significant absolute beta beta of the right parietal region (P4) (P3) and the right parietal lobe (P4), the ratio of the mid-beta to theta (the ratio of the mid-beta to theta) was statistically significantly increased (Korean Patent No. 10-72754). (Combination of Omija essential oil and Chrysanthemum essential oil as an active ingredient), the combined fragrance of Omija essential oil and true angelic essential oil showed an increase in RFA as an EEG indicator in the left parietal lobe during inhalation, (RLB) in the parietal lobe, increase in RST in the right parietal lobe, decrease in SEF90 in the left prefrontal lobe, and increase in ASEF in the right and left parietal lobes (Korean Patent No. 10-2016-0112927).

(Technical Literature 1) EEG is affected by various factors, and natural essential oil is also reported to be a factor affecting EEG. Inhalation of a refreshing lime natural essential oil increased the low-beat wave in the right temporal lobe of the brain and increased the mid-beta wave in the right frontal lobe and left temporal lobe (Lee, 2012. Effect of lime and clove- This result is inferred that volatile flavor compounds contained in the natural essential oils of lime may have been aroused with high attention and cognitive function. In addition, inhalation of Clove natural essential oil increased fast alpha waves in the left and right frontal lobe of the brain, left frontal lobe, right temporal lobe, and mid beta waves in the left parietal lobe, suggesting that the volatile flavor substances contained in the clove (Lee, 2002). The effects of lime flavor and clove flavor on EEG were investigated. (Technical Literature 2) It is known that perfume affects brain waves and generates different brain waves according to kinds of perfume. As a result of stimulation of subjects with LaVentor perfume diluted in grape seed oil, it was reported that the alpha waves and beta waves were increased in the frontal lobes of theta and alfa and in the temporal lobe. These EEG changes were reported to help the subjects relax the tension Cerebral Cortical Activity by Directional Olfactory Stimulation: A Nonparametric Statistical Map Using Low Resolution Electromag- netic Tomography (LOREATA) 22 (4) : 334-339). (2003) reported an increase in ALPHA in both left and right frontal lobes when middle-aged women inhaled a mixture of bergamot, geranium, lavender, and clarysege oil (Kim, JS, 2003. Flavor suction Effects of psychological and physiological responses on middle-aged women. (Technical Literature 4) Seo et al. (2016) measured the EEG changes after inhaling the fragrance of coniferous wood into one nose and both nasal cavities of the subjects. As a result, when one nose was inhaled, Absolute Beta wave and Cetapha decreased, (2002) reported an increase in the absolute alpha and absolute hyaluronan levels, which suggests that the nasal airway changes in different ways depending on which nose is inhaled (Seo M, Sowndhararajan K, Kim S. 2016. Influence of binasal and uninasal inhalations of essential oils of Abies koreana twigs on electroencephalographic activity of human. (Technical Literature 5) Kim et al. (2017) inhaled alpha-pinene and beta-pinene, which are structural isomers having the same chemical formula and molecular weight but different chemical structures, into subjects, As a result of studying the effect of compounds on human brain waves, alpha-pinene showed a significant increase in Absolute Alpha and Absolute Beta waves in women, while beta-pinene increased Absolute High alpha and Beta waves (1999) reported that the effects of inhalation of isomers, (+) - alpha-pinene and (+) - beta-pinene on human electroencephalographic activity According to the European Joural of Integrative Medicine. 17: 33-39).

It is an object of the present invention to provide a combined perfume composition which not only increases the RFA index at the temporal T8 region but also reduces the RG index at the Cp6 region to induce stability of mind and body.

In order to achieve the above object, the combined perfume composition which not only increases the RFA index at the temporal T8 region of the present invention but also decreases the RG index at the Cp6 region to induce the stability of mind and body, comprises (a) (B) a step of examining the preference of the perfume composition, (c) a step of evaluating the fragrance composition, and (d) a step of analyzing the perfume composition.

The perfume composition of the present invention may be selected from the group consisting of chamomile flavor, caraway flavor, lemon balm flavor, silybum marianum flavor, angelica flavor, ethyl alcohol, d-limonene, peppermint essential oil extract, mandarin orange essential oil extract, elder flower absolute, vanilla bean absolute, geranium oil egypt ), Characterized by herbal essence fragrance. When the perfume composition is inhaled, the RFA index is increased at the T8 region of the temporal lobe, and the RG index is decreased at the Cp6 region of the temporal lobe to provide relaxation and stability of mind and body.

The combined perfume composition of the present invention is characterized by increasing the RFA index at the temporal T8 region upon inhalation and decreasing the RG index at the temporal Cp6 region to provide relaxation and stability of the mind and body. Therefore, Can be applied to the development of a variety of fragrance and flavor related industrial products that can be provided to people.

1 is a block diagram of a brain-wave measuring device QEEG-64FX.
FIG. 2 shows the position and designation of 32 points for EEG measurement.
Figure 3 shows a subject wearing an EEG cap for EEG measurement.
Figure 4 shows the 3D mapping of changes in RG power spectrum before and after inhalation of the combination perfume 4 of herbal essence fragrance
Figure 5 shows the 3D mapping of RFA power spectrum changes before inhalation and inhalation of the combined fragrance 4 of the herbal essence fragrance

The contents of the present invention will be described in more detail through the following examples. However, the scope of the present invention is not limited to the following embodiments, but includes modifications of equivalent technical ideas.

Example 1. Herbal essence type fragrance combination using natural fragrance and synthetic fragrance

The raw material of the herbal essence type perfume composition of the present invention is classified into a natural fragrance extracted from natural and a synthetic fragrance obtained through organic synthesis. The natural fragrances used include, but are not limited to, chamomile flavor, caraway flavor, lemon balm flavor, silybum marianum flavor, angelica flavor, peppermint flavor, dermis mandarin orange fragrance, elder flower absolute, vanilla bean absolute, geranium oil egypt, and the synthetic fragrance used was d-limonene.

The compositions and composition ratios of the natural perfume and the synthetic perfume for developing the combination perfume of the herbal essence type are shown in Table 2.

Composition and Composition for Development of Herbal Essence Type Combination Spices Perfume name Composition ratio (%) Combination
Spices
One Combination
Spices
2 Combination
Spices
3 Combination
Spices
4 Combination
Spices
5 Combination
Spices
6 Combination
Spices
7 Combination
Spices
8 natural
Spices Chamomile flavoring 45.00 40.00 35.00 30.00 25.00 20.00 15.00 10.00 Caraway flavoring 6.00 9.00 12.00 15.00 18.00 21.00 24.00 27.00 Lemon balm spices 21.00 19.00 17.00 15.00 13.00 11.00 9.00 7.00 Silyoumi Marinum Spice 6.00 9.00 12.00 15.00 18.00 21.00 24.00 27.00 Angelica spices 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 Mint flavor 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Dermis perfume 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Elder Flower Absolute Flavor 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Vanilla Bean Absolut Flavor 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Geranium Oil Egyptian Fragrance 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 synthesis
Spices D-limonene 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 ethyl alcohol 9.48 9.48 9.48 9.48 9.48 9.48 9.48 9.48 Sum 100 100 100 100 100 100 100 100

Example 2. Investigation of preference of combination fragrance of herbal essence type

Seven expert panel members who had at least 6 months olfactory training were surveyed for their preference for the eight combined fragrances that were steered in Example 1. Experts participating in the second embodiment were all in their twenties, and they were attending college or graduate school. Each combined perfume was sprinkled on a fountain pen (0.5 cm x 13 cm) using an atomizer and perfused at a position 5 cm from the nose and given 10 points for the most preferred perfume and 0 for the most preferred perfume . Table 3 shows the average values based on the preference results.

Preference survey on 8 kinds of herbal essence type combination fragrance preference Composition ratio (%) Combination
Spices
One Combination
Spices
2 Combination
Spices
3 Combination
Spices
4 Combination
Spices
5 Combination
Spices
6 Combination
Spices
7 Combination
Spices
8 medium 4.2 8.1 8.0 9.2 6.0 4.7 5.4 7.6

Example 3. Scent evaluation of combination fragrance 4 of herbal essence type

The fragrance of the combination fragrance 4, which was the most preferred in the second embodiment, was commissioned and evaluated by five professional panels of Rose of Sharon, a perfume development club of Kangwon University. The fragrance was evaluated according to the fragrance evaluation term which distinguishes the fragrance characteristic of the essential oil.

Herbal essence type combination fragrance 4 fragrance Drape type Combination Spices 4 Flavor Aldehyde Animalic Balsamic Citrus ● Coniferous Earthy Floral Fruity ● Green ● Herbal ● Medicinal Minty ● Marine ? (wet) Musky Oily Oriental Powdery Smoky Spicy Sweet (sweet) ● Woody

Example 4. Combination of herbal essence type EEG measurement and data analysis of fragrance 4

It was experimentally examined how the brain waves are changed when humans inhale the combination fragrance 4 having the herbal essence type fragrance of the third embodiment. The instrument used for EEG measurement was QEEG-64FX (LAXTHA Inc. Korea) (Fig. 2). EEG signals were measured using LAXTHA data acquisition equipment and analysis program (Telescan). Subjects were given 32 ground electrodes using the Electro-Gel International Electro-Gel (Electro-Cap Internationals, Inc. USA) for the left and right prefrontal cords (Fp1, Fp2, Fpz , Left and right frontal lobes (F3, F4, F7, F8, Fz, Fc1, Fc2, Fc5, Fc6), left and right temporal lobe (T3, T4, T7, T8, Cp5, Cp6) The left and right parietal lobes (P3, P4, P7, P8, Cp1, Cp2, Cz and Pz) and the left and right occipital lobes (O1, O2 and Oz) (Figs. 1 and 2). The subjects were measured for 45 seconds each with no stimulation in the eyes closed state and inhalation (combined stimulation) of the herbal essence type combination perfume 4.

Figure 1. EEG measurement device QEEG-64FX.

Figure 2. Position and name of 32 points for EEG measurement.

Figure 3. Subjects wearing EEG caps for EEG measurements.

Herbal essence combination The data obtained through EEG measurement for perfume 4 were obtained by extracting only representative 30-second EEG signal among the subjects' EEG signals and quantifying and imaging them by batch processing of brain waves. The statistical analysis software program SPSS (SPSS 23.0 version) was used to perform a corresponding sample T test. Table 5 shows the types of EEG indicators for data analysis in this embodiment, and Table 6 shows the meaning of EEG indices.

Types of EEG indicators used in data analysis No. Analysis index Full name of EEG indicator Waveband (Hz) One AT Absolute Theta Power Spectrum 4 to 8 2 AA Absolute Alpha Power Spectrum 8-13 3 AB Absolute Beta Power Spectrum 13 to 30 4 AG Absolute Gamma Power Spectrum 30 to 50 5 AFA Absolute Fast Alpha Power Spectrum 11-13 6 ASA Absolute Slow Alpha Power Spectrum 8 ~ 11 7 ALB Absolute Low Beta Power Spectrum 12 to 15 8 AMB Absolute Mid Beta Power Spectrum 15-20 9 AHB Absolute High Beta Power Spectrum 20 ~ 30 10 RT Relative Theta Power Spectrum (4 to 8) / (4 to 50) 11 RA Relative Alpha Power Spectrum (8 to 13) / (4 to 50) 12 RB Relative Beta Power Spectrum (13 to 30) / (4 to 50) 13 RG Relative Gamma Power Spectrum (30 to 50) / (4 to 50) 14 RFA Relative Fast Alpha Power Spectrum (11 to 13) / (4 to 50) 15 RSA Relative Slow Alpha Power Spectrum (8 to 11) / (4 to 50) 16 RLB Relative Low Beta Power Spectrum (12 to 15) / (4 to 50) 17 RMB Relative Mid Beta Power Spectrum (15 to 20) / (4 to 50) 18 RHB Relative High Beta Power Spectrum (20 to 30) / (4 to 50) 19 RST Ratio of SMR to Theta (12 to 15) / (4 to 8) 20 RMT (Ratio of Mid Beta to Theta) (15 to 20) / (4 to 8) 21 RSMT Ratio of (SMR ~ Mid Beta) to Theta (12 to 20) / (4 to 8) 22 RAHB Ratio of Alpha to High Beta (8 to 13) / (20 to 30) 23 SEF50 Spectral Edge Frequency 50% 4 to 50 24 SEF 90 Spectral Edge Frequency 90% 4 to 50 25 ASEF Spectral Edge Frequency 50% of Alpha Spectrum Band 8-13

Meaning of EEG indices used in data analysis No. Analysis index meaning One AT Absolute power of theta wave (meditation, sleepy) 2 AA Absolute power of Alpha wave (relaxation, stability) 3 AB The absolute power of Beta waves (tension, arousal, activity, cognitive function) 4 AG Absolute power of the Gamma wave (highly cognitive or anxious, excited) 5 AFA Fast alpha Wave's absolute power (relaxed, immersive, creativity) 6 ASA Absolute power of Slow alpha wave (relaxation) 7 ALB Absolute power of the low beta wave, SMR parameter (caution or boundary) 8 AMB Mid beta wave absolute power (concentration, attention) 9 AHB High beta Absolute power (stress, mental load, tension) 10 RT Percentage of theta waves in total 11 RA The ratio of alpha waves in total 12 RB Ratio of beta waves in total 13 RG The ratio of gamma waves in total 14 RFA The ratio of fast alpha waves in total 15 RSA The ratio of slow alpha wave in total 16 RLB The ratio of low beta waves in total 17 RMB The ratio of mid beta waves in total 18 RHB The ratio of high beta waves in total 19 RST The ratio of theta wave in SMR wave 20 RMT The ratio of theta wave in mid beta waves 21 RSMT SMR ~ mid beta wave / theta wave (EEG concentration indicator) 22 RAHB Alpha wave / high beta wave (stable and relaxed) 23 SEF50 Median Frequency Brain activity (EEG) 24 SEF 90 Median Frequency Brain activity (mental stress, stress) 25 ASEF Alpha wave SEF50 (comfort: fast alpha waves)

As a result of examining the effect of the combination fragrance 4 having the highest preference in the present invention 2 on human brain waves, there was a statistically significant difference between the RG and RFA indicators in 25 EEG indices (p <0.05) There was no difference in the indicators. Therefore, only the RG and RFA indicators showing statistically significant differences are described in this embodiment.

The combination perfume 4, which had the highest preference in Example 2 of the present invention, significantly decreased the RG index in the temporal Cp6 region (p < 0.05), and the results are shown in Table 7. The resulting image is shown in Figure 4 as 3D mapping. The RG indicator is the absolute power ratio of the gamma band to the absolute power of the entire frequency domain in the EEG spectrum, which is the spectral value divided by the gamma wave range (30 to 50 Hz) into the entire frequency range (4 to 50 Hz). Gamma waves are among the fastest of the brain activity waves, and are said to be activated when they are emotionally irritated, or when they are highly cognitive such as reasoning and judgment.

Combination of Herbal Essence Flavor 4 Changes in relative gamma (RG) power spectrum before inhalation and inhalation Relative gamma power spectrum (RG) Site Inhalation N Mean SD t-test p Cp6 Before 20 0.2031 0.0794 2.1245 0.0470 During 20 0.1763 0.0680

FIG. 4 shows a graphical representation of the change in the RG power spectrum by the combined fragrance 4 of the herbal essence fragrance of the present embodiment as a brain map. It can be seen that the Cp6 region of the right temporal lobe was significantly changed from red to green during inhalation and before inhalation of the combination perfume 4.

Figure 4. Combination of herbal essence fragrance Fragrance 4 3D mapping of changes in RG power spectrum before inhalation and inhalation

The combination perfume 4, which was the most preferred in Example 2 of the present invention, increased the RFA index statistically significantly in the temporal T8 region (p <0.05). The results are shown in Table 8. The resulting image is shown in Figure 5 as 3D mapping. The RFA indicator is the absolute power ratio of the fast alpha band to the absolute power of the entire frequency domain in the EEG spectrum, which is the relative power spectrum value divided by the entire frequency domain (4 to 50 Hz) of the fast alpha waveband (11 to 13 Hz) do.

The Combination Fragrance 4 of the Herbal Essence Scent of the Invention is evaluated to make the brain psychologically relaxed and relaxed.

Combination of herbal essence fragrance Spices 4 Changes in relative fast alpha (RFA) power spectrum before inhalation and inhalation Relative fast alpha power spectrum (RFA) Site Inhalation N Mean SD t-test p T8 Before 20 0.0558 0.0181 -2.2774 0.0345 During 20 0.0666 0.0227

FIG. 5 shows the change in the RFA power spectrum by the combination perfume 4 of the herbal essence fragrance of the present embodiment as a brain map. It can be seen that the combination perfume 4 was significantly changed from blue to red in the right temporal lobe T8 region before inhalation and during inhalation.

Figure 5. Combination of herbal essence fragrance 3D mapping of RFA power spectrum changes before inhalation and inhalation of perfume 4

The results of EEG analysis of Example 4 showed that the combination perfume 4 exhibiting the herbal essence scent changed the EEG changes statistically in the temporal lobe among the brain regions of the subjects. The temporal lobe of the brain area accounts for about 22% of the human cerebral cortex volume and is well known as the hearing area for language processing. The auditory cortex is located in the upper chambers of the temporal lobes and inside the sciatic parenchyma, and immediately after the auditory cortex is the area of Wernicke, which understands human language. In addition, temporal lobe plays an important role in memory function, and is also known as a source of faith and a source of religious faith.

The EEG changes of Example 4 were produced by the volatile organic compounds of herbal essence fragrance contained in the combination fragrance 4. When the subjects inhale the combination fragrance 4, the volatile organic compounds contained therein are mixed with the olfactory epithelium ), Which binds to the olfactory binding protein, and the resulting electrical signal reaches the olfactory bulb along the axon. The olfactory lumen is located in the frontal region of the brain. It is known that electrical signals are transmitted to the olfactory tract through the olfactory tract and are transmitted from the olfactory cortex to the prefrontal cortex (RJ Zatorre , M. Jones-Gotman, AC Evans and E. Meyer, 1992. Functional localization and lateralization of human olfactory cortex, Nature 360: 339-340, R. Axel 1995. The molecular logic of smell. And the sensory perception of olfactory sensation, the Korean Society for Biotechnology Biotechnology 13 (6): 631-637), the electrical signal by the combination fragrance 4 is also transmitted to the temporal lobe along the olfactory passage mentioned above .

Applicable to the fragrance industrial and flavor industries

Claims (3)

(W / w), 15.00% (w / w) of lemon balm, 15.00% (w / w) of lemon balm, 15.00% w / w), mint flavor 0.15% (w / w), dermis flavor 0.15% (w / w), elder flower absolute 0.05% (w / w), vanilla bean absolute 0.05% (w / A fragrance composition of herbal essence fragrance containing 0.02% (w / w) of d-limonene, 0.10% (w / w) of d-limonene, and 9.48%
delete The method according to claim 1, wherein the EEG-mediated EEG is used to statistically significantly reduce the RG index at the Cp6 region of the temporal lobe, and statistically significantly increase the RFA index at the temporal T8 region, Perfume composition

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