KR102340691B1 - 생체 마이크로 대상물용 펜 - Google Patents

생체 마이크로 대상물용 펜 Download PDF

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KR102340691B1
KR102340691B1 KR1020217002166A KR20217002166A KR102340691B1 KR 102340691 B1 KR102340691 B1 KR 102340691B1 KR 1020217002166 A KR1020217002166 A KR 1020217002166A KR 20217002166 A KR20217002166 A KR 20217002166A KR 102340691 B1 KR102340691 B1 KR 102340691B1
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pen
micro
objects
pens
flow
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KR20210010965A (ko
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케빈 티 채프만
이고르 와이 칸도르스
개탄 엘 매튜
제이 테너 네빌
밍 씨 우
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버클리 라잇츠, 인크.
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0454—Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Fluid Mechanics (AREA)
  • Clinical Laboratory Science (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020217002166A 2012-10-31 2013-10-30 생체 마이크로 대상물용 펜 Active KR102340691B1 (ko)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201261720956P 2012-10-31 2012-10-31
US61/720,956 2012-10-31
US14/060,117 2013-10-22
US14/060,117 US9857333B2 (en) 2012-10-31 2013-10-22 Pens for biological micro-objects
KR1020197019338A KR102208585B1 (ko) 2012-10-31 2013-10-30 생체 마이크로 대상물용 펜
PCT/US2013/067479 WO2014070873A1 (en) 2012-10-31 2013-10-30 Pens for biological micro-objects

Related Parent Applications (1)

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KR1020197019338A Division KR102208585B1 (ko) 2012-10-31 2013-10-30 생체 마이크로 대상물용 펜

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Publication Number Publication Date
KR20210010965A KR20210010965A (ko) 2021-01-28
KR102340691B1 true KR102340691B1 (ko) 2021-12-17

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KR1020217002166A Active KR102340691B1 (ko) 2012-10-31 2013-10-30 생체 마이크로 대상물용 펜
KR1020157014290A Expired - Fee Related KR101998918B1 (ko) 2012-10-31 2013-10-30 생체 마이크로 대상물용 펜
KR1020197019338A Active KR102208585B1 (ko) 2012-10-31 2013-10-30 생체 마이크로 대상물용 펜

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KR1020197019338A Active KR102208585B1 (ko) 2012-10-31 2013-10-30 생체 마이크로 대상물용 펜

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US (4) US9857333B2 (enExample)
EP (2) EP2914965B1 (enExample)
JP (3) JP6529908B2 (enExample)
KR (3) KR102340691B1 (enExample)
CN (2) CN108070582B (enExample)
AU (3) AU2013337937B2 (enExample)
CA (1) CA2888810C (enExample)
DK (1) DK2914965T3 (enExample)
IL (2) IL272535B2 (enExample)
SG (2) SG11201600580PA (enExample)
WO (1) WO2014070873A1 (enExample)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201602336UA (en) 2013-10-22 2016-05-30 Berkeley Lights Inc Micro-fluidic devices for assaying biological activity
DK3060645T3 (en) * 2013-10-22 2019-04-08 Berkeley Lights Inc MICROFLUID DEVICES WITH INSULATION DISEASES AND METHODS FOR TESTING BIOLOGICAL MICRO-OBJECTS
SG10202006525PA (en) 2013-10-22 2020-08-28 Berkeley Lights Inc Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
US20150166326A1 (en) 2013-12-18 2015-06-18 Berkeley Lights, Inc. Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device
US20150306599A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. Providing DEP Manipulation Devices And Controllable Electrowetting Devices In The Same Microfluidic Apparatus
US20150306598A1 (en) * 2014-04-25 2015-10-29 Berkeley Lights, Inc. DEP Force Control And Electrowetting Control In Different Sections Of The Same Microfluidic Apparatus
US11192107B2 (en) 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
WO2015188171A1 (en) 2014-06-06 2015-12-10 Berkeley Lights, Inc. Isolating microfluidic structures and trapping bubbles
US9815056B2 (en) 2014-12-05 2017-11-14 The Regents Of The University Of California Single sided light-actuated microfluidic device with integrated mesh ground
TWI721545B (zh) 2014-12-08 2021-03-11 美商柏克萊燈光有限公司 側向式/垂直式電晶體結構及其製造與使用方法
CN110918142B (zh) * 2014-12-08 2022-10-25 伯克利之光生命科技公司 微流体装置中定向流动的致动微流体结构及使用它的方法
CN107223208B (zh) 2014-12-09 2021-04-09 伯克利之光生命科技公司 微流体装置中微物体的自动检测和重新定位
CN107206377B (zh) * 2014-12-09 2020-02-11 伯克利之光生命科技公司 微流体装置中测定阳性的区域的自动检测
US9744533B2 (en) * 2014-12-10 2017-08-29 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
SG11201704659PA (en) * 2014-12-10 2017-07-28 Berkeley Lights Inc Systems for operating electrokinetic devices
CA2977546A1 (en) * 2015-03-04 2016-09-09 Berkeley Lights, Inc. Generation and selection of embryos in vitro
HK1246623A1 (zh) * 2015-03-04 2018-09-14 伯克利之光生命科技公司 体外胚胎的生殖和选择
SG11201708429WA (en) 2015-04-22 2017-11-29 Berkeley Lights Inc Microfluidic cell culture
TWI819383B (zh) * 2015-04-22 2023-10-21 美商伯克利之光生命科技公司 微流體器件及在該微流體器件中培養生物細胞之方法
US10751715B1 (en) 2015-04-22 2020-08-25 Berkeley Lights, Inc. Microfluidic reporter cell assay methods and kits thereof
CA2982931C (en) * 2015-04-22 2021-10-19 Berkeley Lights, Inc. Freezing and archiving cells on a microfluidic device
US10101250B2 (en) 2015-04-22 2018-10-16 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
WO2017075295A1 (en) * 2015-10-27 2017-05-04 Berkeley Lights, Inc. Microfluidic electrowetting device apparatus having a covalently bound hydrophobic surface
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
KR20180085783A (ko) 2015-11-23 2018-07-27 버클리 라잇츠, 인크. 인 시츄-생성된 미세유체 격리 구조체들, 키트들, 및 그 이용 방법들
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
KR20220153685A (ko) 2015-12-08 2022-11-18 버클리 라잇츠, 인크. 미세유체 디바이스들 및 키트들 및 그것의 사용을 위한 방법들
CN108698814A (zh) * 2015-12-30 2018-10-23 伯克利之光生命科技公司 用于光学驱动的对流和移位的微流体设备、试剂盒及其方法
TWI808934B (zh) 2015-12-31 2023-07-21 美商伯克利之光生命科技公司 經工程化以表現促發炎多肽之腫瘤浸潤細胞
TWI756199B (zh) * 2016-01-15 2022-03-01 美商伯克利之光生命科技公司 製造患者專ㄧ性抗癌治療劑之方法及使用該治療劑之治療方法
CN109922885B (zh) 2016-03-16 2022-05-10 伯克利之光生命科技公司 用于基因组编辑克隆的选择和传代的方法、系统和装置
CN109196094A (zh) 2016-03-17 2019-01-11 伯克利之光生命科技公司 微流体装置中t淋巴细胞的选择和克隆
KR20220139426A (ko) 2016-03-31 2022-10-14 버클리 라잇츠, 인크. 핵산 안정화 시약, 키트들, 및 그 이용 방법들
CN109642869B (zh) 2016-04-15 2022-04-12 伯克利之光生命科技公司 用于坞内测定的方法、系统和试剂盒
US10675625B2 (en) 2016-04-15 2020-06-09 Berkeley Lights, Inc Light sequencing and patterns for dielectrophoretic transport
IL263274B2 (en) 2016-05-26 2023-10-01 Berkeley Lights Inc Covalently adapted surfaces, kits and methods for their production and uses
US10710075B2 (en) * 2016-06-21 2020-07-14 Quantumdx Group Limited Target cell concentration using dielectrophoresis (DEP)
KR102456488B1 (ko) 2016-07-21 2022-10-19 버클리 라잇츠, 인크. 미세유체 디바이스에서의 t 림프구들의 분류
EP3519612B1 (en) 2016-10-01 2022-04-06 Berkeley Lights, Inc. Dna barcode compositions and methods of in situ identification in a microfluidic device
KR102669330B1 (ko) 2016-12-01 2024-05-28 버클리 라잇츠, 인크. 미세유체 디바이스에서의 마이크로-객체의 자동화된 검출 및 재포지셔닝
CA3045334C (en) 2016-12-01 2023-11-21 Berkeley Lights, Inc. Apparatuses, systems and methods for imaging micro-objects
US11473081B2 (en) 2016-12-12 2022-10-18 xCella Biosciences, Inc. Methods and systems for screening using microcapillary arrays
AU2017388874A1 (en) 2016-12-30 2019-07-18 Berkeley Lights, Inc. Methods for selection and generation of genome edited T cells
US11156626B2 (en) 2016-12-30 2021-10-26 xCella Biosciences, Inc. Multi-stage sample recovery system
JPWO2018207454A1 (ja) * 2017-05-12 2020-03-19 株式会社ニコン 対象物操作装置、及び対象物操作方法
KR20200026878A (ko) 2017-06-06 2020-03-11 지머젠 인코포레이티드 균류 균주를 개량하기 위한 htp 게놈 공학 플랫폼
TWI832820B (zh) 2017-07-21 2024-02-21 美商伯克利之光生命科技公司 抗原呈現合成表面、共價功能化表面、經活化之t細胞及其用途
WO2019067319A2 (en) * 2017-09-29 2019-04-04 Schlumberger Technology Corporation MICRO-FLUIDIC TECHNIQUE FOR MULTI-CONTACT MISCIBILITY DETECTION
WO2019075476A2 (en) 2017-10-15 2019-04-18 Berkeley Lights, Inc. METHODS, SYSTEMS AND KITS FOR ENCLOSED TESTS
CN108300654B (zh) * 2018-02-09 2021-08-17 华中科技大学同济医学院附属协和医院 评估可移动生物活体药代动力学的芯片及实时检测系统
DE102018203047A1 (de) * 2018-03-01 2019-09-05 Robert Bosch Gmbh Mikrofluidische Vorrichtung
US11041834B2 (en) 2018-03-06 2021-06-22 Waters Technologies Corporation High surface area chromatographic device with low pressure drop
WO2019232473A2 (en) 2018-05-31 2019-12-05 Berkeley Lights, Inc. Automated detection and characterization of micro-objects in microfluidic devices
EP3810750A4 (en) 2018-06-06 2022-03-23 Zymergen, Inc. Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production
CA3054046A1 (en) * 2018-09-04 2020-03-04 The Governing Council Of The University Of Toronto Patterned optoelectronic tweezers
GB2577074B (en) 2018-09-12 2022-06-01 Quantumdx Group Ltd Microfluidic device with DEP arrays
CA3112333A1 (en) * 2018-09-14 2020-03-19 Berkeley Lights, Inc. Methods for assaying binding affinity
CA3112813A1 (en) 2018-09-21 2020-03-26 Berkeley Lights, Inc. Functionalized well plate, methods of preparation and use thereof
EP3864128A4 (en) 2018-10-10 2022-07-06 Stamm Vegh Corporation CONTINUOUS CURRENT MICROBIOREACTOR
CN113195085B (zh) 2018-10-11 2024-04-19 伯克利之光生命科技公司 用于识别优化蛋白产生的系统和方法以及其试剂盒
SG11202103296RA (en) 2018-10-18 2021-05-28 Berkeley Lights Inc Proto-antigen-presenting synthetic surfaces, activated t cells, and uses thereof
AU2019371417A1 (en) 2018-11-01 2021-06-17 Berkeley Lights, Inc. Methods for assaying biological cells in a microfluidic device
SG11202104544WA (en) 2018-11-19 2021-06-29 Berkeley Lights Inc Microfluidic device with programmable switching elements
EP3890876B1 (en) 2018-12-06 2024-05-01 Xcella Biosciences, Inc. Lateral loading of microcapillary arrays
WO2020168258A1 (en) 2019-02-15 2020-08-20 Berkeley Lights, Inc. Laser-assisted repositioning of a micro-object and culturing of an attachment-dependent cell in a microfluidic environment
WO2020223555A1 (en) 2019-04-30 2020-11-05 Berkeley Lights, Inc. Methods for encapsulating and assaying cells
US20220226829A1 (en) * 2019-06-05 2022-07-21 Amgen Inc. Systems for performing cellular analysis and related devices for conditioning environments adjacent chips in such systems
CN110618095A (zh) * 2019-11-08 2019-12-27 武汉大学深圳研究院 一种光流控水体溶解氧探测器
JP2023503253A (ja) 2019-11-17 2023-01-27 バークレー ライツ，インコーポレイテッド 生体試料を分析するためのシステム及び方法
UY39063A (es) 2020-02-03 2021-08-31 Stamm Vegh Corp Plataforma, sistemas y dispositivos para impresiones 3d
WO2021183458A2 (en) 2020-03-09 2021-09-16 Berkeley Lights, Inc. Methods, systems and kits for in-pen assays
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
EP4204810A4 (en) 2020-09-07 2024-10-09 Berkeley Lights, Inc. METHODS FOR DOSING A BIOLOGICAL CELL
WO2023032827A1 (ja) 2021-08-31 2023-03-09 キヤノン株式会社 ポリウレタンを含む非発泡の成形体
EP4239333A1 (en) 2022-03-03 2023-09-06 Lonza Biologics plc Method for selecting clonal cells expressing correctly assembled multi-specific binding molecules
WO2024076923A1 (en) * 2022-10-03 2024-04-11 Bruker Cellular Analysis, Inc. Micro-object displacement within a microfluidic environment

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005521425A (ja) 2002-04-01 2005-07-21 フルイディグム コーポレイション 微小流体粒子分析システム
JP2007537729A (ja) * 2004-04-12 2007-12-27 ザ・レジェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア 微粒子及び細胞の操作用の光電子ピンセット
US20110033910A1 (en) 2008-04-15 2011-02-10 Tosoh Corporation Cell selection apparatus, and cell selection method using the same
WO2011149032A1 (ja) * 2010-05-26 2011-12-01 東ソー株式会社 生体試料固定装置
WO2011160430A1 (en) * 2010-06-25 2011-12-29 Capitalbio Corporation Integrated microfluidic device for single oocyte trapping
US20130099143A1 (en) 2010-06-30 2013-04-25 Tosoh Corporation Structure for particle immobilization and apparatus for particle analysis

Family Cites Families (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2288760T3 (es) 1996-04-25 2008-01-16 Bioarray Solutions Ltd. Ensamblaje electrocinetico controlado por luz de particulas proximas a superficies.
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6942776B2 (en) 1999-05-18 2005-09-13 Silicon Biosystems S.R.L. Method and apparatus for the manipulation of particles by means of dielectrophoresis
NZ533466A (en) 1999-06-28 2005-10-28 California Inst Of Techn Microfabricated elastomeric valve and pump systems
US20030007894A1 (en) 2001-04-27 2003-01-09 Genoptix Methods and apparatus for use of optical forces for identification, characterization and/or sorting of particles
ITTO20010411A1 (it) 2001-05-02 2002-11-02 Silicon Biosystems S R L Metodo e dispositivo per l'esecuzione di test e saggi ad alta processivita' ed alto valore biologico su cellule e/o composti.
US7075162B2 (en) * 2001-08-30 2006-07-11 Fluidigm Corporation Electrostatic/electrostrictive actuation of elastomer structures using compliant electrodes
CN1291035C (zh) * 2001-10-09 2006-12-20 清华大学 用于样品制备和分析的集成式生物芯片系统
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
US6958132B2 (en) 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
US7790443B2 (en) 2002-08-27 2010-09-07 Vanderbilt University Bioreactors with substance injection capacity
WO2004065618A2 (en) 2003-01-16 2004-08-05 Thermogenic Imaging Methods and devices for monitoring cellular metabolism in microfluidic cell-retaining chambers
WO2004089810A2 (en) 2003-04-03 2004-10-21 Fluidigm Corp. Microfluidic devices and methods of using same
DE112004001376D2 (de) * 2003-05-19 2006-04-13 Knoell Hans Forschung Ev Vorrichtung und Verfahren zur Strukturierung von Flüssigkeiten und zum zudosieren von Reaktionsflüssigkeiten zu in Separationsmedium eingebetteten Flüssigkeitskompartimenten
JP4328168B2 (ja) 2003-10-02 2009-09-09 ソニー株式会社 毛細管現象を利用する物質間の相互作用検出部と該検出部を用いる方法及びバイオアッセイ用基板
WO2005072399A2 (en) 2004-01-29 2005-08-11 Massachusetts Institute Of Technology Microscale sorting cytometer
WO2006037033A2 (en) 2004-09-24 2006-04-06 The Regents Of The University Of California A microfluidic device for enabling the controlled growth of cells
US20060091015A1 (en) 2004-11-01 2006-05-04 Applera Corporation Surface modification for non-specific adsorption of biological material
CN101208422A (zh) 2005-02-23 2008-06-25 威廉·A·库克澳大利亚股份有限公司 培养装置
EP1904619A4 (en) 2005-07-07 2012-05-02 Univ California METHOD AND DEVICE FOR CELL CULTURE ARRAY
CN101548004A (zh) * 2005-08-19 2009-09-30 加利福尼亚大学董事会 用于诊断学和细胞分析的微流体方法
US20100003666A1 (en) 2005-08-19 2010-01-07 The Regents Of The University Of California Microfluidic Methods for Diagnostics and Cellular Analysis
WO2007102839A2 (en) 2005-10-27 2007-09-13 Applera Corporation Optoelectronic separation of biomolecules
US8124015B2 (en) 2006-02-03 2012-02-28 Institute For Systems Biology Multiplexed, microfluidic molecular assay device and assay method
WO2008089493A2 (en) 2007-01-19 2008-07-24 Fluidigm Corporation High precision microfluidic devices and methods
WO2008119066A1 (en) 2007-03-28 2008-10-02 The Regents Of The University Of California Single-sided lateral-field and phototransistor-based optoelectronic tweezers
ATE554859T1 (de) 2007-05-24 2012-05-15 Univ California Integrierte fluidische vorrichtungen mit magnetischer sortierung
EP2078958A1 (en) 2007-10-25 2009-07-15 Koninklijke Philips Electronics N.V. A reconfigurable microfluidic filter based on eletric fields
WO2009146143A2 (en) 2008-04-03 2009-12-03 The Regents Of The University Of California Ex-vivo multi-dimensional system for the separation and isolation of cells, vesicles, nanoparticles and biomarkers
WO2009130694A2 (en) 2008-04-21 2009-10-29 Cell Kinetics Ltd. Flat cell carriers with cell traps
CN101821808B (zh) 2008-07-28 2014-03-19 艾格瑞系统有限公司 用于确定头组件与存储介质之间的飞行高度的系统和方法
GB2464300A (en) 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
EP2414539B1 (en) 2009-04-03 2020-12-16 The Regents of The University of California Apparatus and method for sorting cells and other biological particulates
US20100273681A1 (en) 2009-04-27 2010-10-28 Wisconsin Alumni Research Foundation Combinatorial chemistry reaction cell with optical tweezers
GB0909923D0 (en) 2009-06-09 2009-07-22 Oxford Gene Tech Ip Ltd Picowell capture devices for analysing single cells or other particles
GB0910330D0 (en) * 2009-06-16 2009-07-29 Univ Leiden A biological microfluidics chip and related methods
JP2011000079A (ja) * 2009-06-19 2011-01-06 Univ Of Tokyo 粒子を操作する方法及びマイクロ流体装置
CN201834907U (zh) * 2010-04-15 2011-05-18 四川国兴盛生物科技有限公司 半封闭式围栏
US10087408B2 (en) 2010-07-07 2018-10-02 The University Of British Columbia System and method for microfluidic cell culture
US9188593B2 (en) 2010-07-16 2015-11-17 The University Of British Columbia Methods for assaying cellular binding interactions
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns
JP5804620B2 (ja) * 2010-08-09 2015-11-04 一般財団法人生産技術研究奨励会 電気的機能を持つマイクロチャンバーアレイ装置およびそれを用いた検査対象物解析方法
JP5920895B2 (ja) 2010-09-14 2016-05-25 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア マイクロ流体捕獲渦を使用して不均一溶液から細胞を単離する方法及びデバイス
US10571475B2 (en) 2010-12-03 2020-02-25 Cellply S.R.L. Rapid screening of monoclonal antibodies
ES2641524T3 (es) 2010-12-03 2017-11-10 Cellply S.R.L. Microanálisis de la función celular
CN102580794B (zh) * 2011-01-13 2014-03-19 博奥生物有限公司 可定位细胞及生物体的微流控芯片及其应用
US9227200B2 (en) 2011-06-03 2016-01-05 The Regents Of The University Of California Microfluidic devices with flexible optically transparent electrodes
CA2842359A1 (en) 2011-08-01 2013-02-07 Denovo Sciences Cell capture system and method of use
WO2013148745A1 (en) 2012-03-28 2013-10-03 Northeastern University Nanofluidic device for isolating, growing, and characterizing microbial cells
KR102257305B1 (ko) 2013-03-28 2021-05-28 더 유니버시티 오브 브리티쉬 콜롬비아 미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005521425A (ja) 2002-04-01 2005-07-21 フルイディグム コーポレイション 微小流体粒子分析システム
JP2007537729A (ja) * 2004-04-12 2007-12-27 ザ・レジェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア 微粒子及び細胞の操作用の光電子ピンセット
US20110033910A1 (en) 2008-04-15 2011-02-10 Tosoh Corporation Cell selection apparatus, and cell selection method using the same
WO2011149032A1 (ja) * 2010-05-26 2011-12-01 東ソー株式会社 生体試料固定装置
WO2011160430A1 (en) * 2010-06-25 2011-12-29 Capitalbio Corporation Integrated microfluidic device for single oocyte trapping
US20130099143A1 (en) 2010-06-30 2013-04-25 Tosoh Corporation Structure for particle immobilization and apparatus for particle analysis

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