JP6529908B2 - 生物学的微小物体用ペン - Google Patents
生物学的微小物体用ペン Download PDFInfo
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Description
Claims (43)
- 基板、及び前記基板の表面上に配置されたマイクロ流体構造を備えるハウジングを備え、
前記基板及び前記マイクロ流体構造が、第1の液状媒体及び前記第1の液状媒体中に懸濁された生物学的微小物体を保持するための内部チャンバを定義し、
前記マイクロ流体構造が、
(i)前記第1の液状媒体のための流路を提供するチャネルと、
(ii)複数の有形の保持ペンと
を備え、
前記複数の保持ペンのそれぞれは、
内部空間を取り囲む筐体であって、第2の液状媒体中に懸濁された生物学的微小物体を保持するように構成された筐体と、
前記流路への単一の開口部と、
を備え、
前記開口部が、いずれの部分も前記チャネルの前記流路と直接対向しないよう配向されており、それにより、前記チャネルが前記第1の液状媒体の流れを含み、前記保持ペンが前記第2の液状媒体を含むときに、前記第1の液状媒体と前記内部空間内の前記第2の液状媒体との拡散混合を可能にし、前記内部空間内の前記第2の液状媒体への前記第1の液状媒体の直接流入が妨げられ、
前記基板の表面が、選択的に活性化及び非活性化されるよう構成された誘電泳動(DEP)電極を備える、
マイクロ流体デバイス。 - (i)前記DEP電極が配線による電気的接続を備えるか、
(ii)前記DEP電極が光導電層を備えるか、あるいは
(iii)前記DEP電極がフォトトランジスタを備える、
請求項1に記載のマイクロ流体デバイス。 - 前記DEP電極が、光学的に制御される、請求項2に記載のマイクロ流体デバイス。
- 1つ以上の微小物体が懸濁している液状媒体の液滴を移動させるためのエレクトロウェッティング電極機構をさらに備える、請求項1に記載のマイクロ流体デバイス。
- 前記エレクトロウェッティング電極機構が、光電子ウェッティング(OEW)装置を備える、請求項4に記載のマイクロ流体デバイス。
- 前記複数の保持ペンが、前記複数の保持ペンの前記開口部が前記流路にすぐ隣接するように前記ハウジング内に配設される、請求項1に記載のマイクロ流体デバイス。
- 前記複数の保持ペンの前記開口部が、前記チャネルに直接接続される、
請求項6に記載のマイクロ流体デバイス。 - 複数のチャネルを備える、請求項7に記載のマイクロ流体デバイス。
- 前記複数の保持ペンのうちの少なくとも1つの保持ペンが、前記開口部から前記筐体内へと延びる内壁を備える、請求項1に記載のマイクロ流体デバイス。
- 前記少なくとも1つの保持ペンの前記内壁が、前記保持ペンの前記開口部と前記保持ペンの前記筐体内の内部収容空間との間のバリアを備える、請求項9に記載のマイクロ流体デバイス。
- 前記複数の保持ペンのうちの少なくとも1つの保持ペンが、筐体と開口部とを備えた内ペンをさらに備え、
前記内ペンは、前記少なくとも1つの保持ペンの前記筐体の内部に配設される、請求項1に記載のマイクロ流体デバイス。 - 前記複数の保持ペンのうちの少なくとも1つの保持ペンが、当該少なくとも1つの保持ペンの前記筐体の内部に配設された内壁により分離された複数の保持空間を備え、
前記複数の保持空間のそれぞれは、前記生物学的微小物体のうち1つを保持するように構成される、請求項1に記載のマイクロ流体デバイス。 - 前記複数の保持ペンの前記開口部のいずれかにおいて誘電泳動(DEP)扉を選択的に作り出すとともに除去する手段をさらに備える、請求項1に記載のマイクロ流体デバイス。
- 前記内部チャンバへと続く前記ハウジングへの注入口と、
前記内部チャンバから続く前記ハウジングからの排出口と、
をさらに備える、請求項1に記載のマイクロ流体デバイス。 - 前記流路と前記複数の保持ペンが、前記注入口と前記排出口との間の前記内部チャンバ内に配設される、請求項14に記載のマイクロ流体デバイス。
- 前記注入口と前記複数の保持ペンとの間の前記内部チャンバ内に配設された気体交換器をさらに備える、請求項15に記載のマイクロ流体デバイス。
- 前記ハウジング内に配設された気体交換器をさらに備える、請求項1に記載のマイクロ流体デバイス。
- 前記ハウジングの全部又は一部が、気体透過性である、請求項1に記載のマイクロ流体デバイス。
- 前記ハウジングが可撓性材料を備える、請求項1に記載のマイクロ流体デバイス。
- 前記複数のペン内の前記生物学的微小物体の生物活動を監視するセンサをさらに備える、請求項1に記載のマイクロ流体デバイス。
- 前記センサが、前記内部チャンバ内の媒体の温度、化学組成、pH又はオスモル濃度を検出する、請求項20に記載のマイクロ流体デバイス。
- 生物学的微小物体の処理方法であって、
請求項1に記載のマイクロ流体デバイスの前記複数の保持ペンの前記内部空間に個々の生物学的微小物体を能動的に置くことと、
ある期間にわたって前記チャネルに前記第1の液状媒体の流れを提供することと、
前記流れを提供している間、前記流れから前記保持ペンの前記内部空間への前記第1の液状媒体の直接流入を妨げることと、
を含む方法。 - 前記第1の液状媒体の前記流れが、前記複数の保持ペンのそれぞれの単一の開口部によって流れる、請求項22に記載の方法。
- 前記期間中に前記複数の保持ペン内の前記生物学的微小物体の特徴を監視するステップをさらに備える、請求項22に記載の方法。
- 前記特徴が、前記複数の保持ペン内の前記生物学的微小物体の生物活動である、請求項24に記載の方法。
- 前記生物活動が、クローン化を含み、
前記監視するステップが、前記複数の保持ペンにおける前記生物学的微小物体の前記クローン化を監視することを含む、
請求項25に記載の方法。 - 前記生物活動が、分泌物を分泌することを含み、
前記監視するステップが、前記複数の保持ペンにおける前記生物学的微小物体の前記分泌物を監視することを含む、請求項25に記載の方法。 - 前記第1の液状媒体が、前記第2の液状媒体とは異なる種類の液状媒体である、請求項22に記載の方法。
- 前記マイクロ流体デバイス内の複数の生物学的微小物体から所定の特徴を有する生物学的微小物体のサブセットを選択するステップをさらに備え、
前記能動的に置くステップが、前記生物学的微小物体の前記選択されたサブセットのみを前記複数の保持ペン内に置くことを含む、請求項22に記載の方法。 - 前記複数の生物学的微小物体が、前記マイクロ流体デバイス内の共通の場所に配置され、
前記能動的に置くステップが、前記選択されたサブセット中の生物学的微小物体の1つ1つを個々に前記共通の場所から前記複数の保持ペンのうち1つに移動させることを含む、請求項29に記載の方法。 - 前記能動的に置くステップが、
前記複数の保持ペンの各々の内部に前記個々の生物学的微小物体のうち1つだけを置くことを含むか、
保持ペンの複数の保持空間のそれぞれに生物学的微小物体を置くことを含むか、又は
前記複数の保持ペンのそれぞれに異なる種類の生物学的微小物体を置くことを含む、
請求項22の方法。 - 前記能動的に置くステップが、前記個々の生物学的微小物体にかかる誘電泳動(DEP)力を作り出すDEP電極を活性化することを含む、請求項22に記載の方法。
- 前記DEP電極を活性化するステップが、光電子ピンセット装置によって前記DEP電極を活性化することを含む、請求項32に記載の方法。
- 前記DEP力が、前記個々の生物学的微小物体と反発する、請求項33に記載の方法。
- 前記能動的に置くステップが、エレクトロウェッティング電極装置を選択的に活性化することによって、中に懸濁している1つ以上の生物学的微小物体を含む媒体の液滴を移動させることを含む、請求項22に記載の方法。
- 前記エレクトロウェッティング電極装置が、光電子ウェッティング(OEW)装置である、請求項35に記載の方法。
- 前記複数の保持ペン内の生物学的微小物体のセットを選択するステップをさらに含み、前記生物学的微小物体のセットが、生物活動又は生物状態に関連する所定の基準、閾値、又は条件を満たす、請求項22に記載の方法。
- 前記複数の保持ペン内の前記生物学的微小物体のセットをさらに処理又は利用するステップを含む、請求項37に記載の方法。
- 前記複数の保持ペンから前記生物学的微小物体のセットを除去するステップをさらに含む、請求項37に記載の方法。
- 前記除去するステップが、DEP装置を備える電極機構を活性化することを含む、請求項39に記載の方法。
- 前記電極機構を活性化するステップが、光学的に制御される、請求項40に記載の方法。
- 前記除去するステップが、エレクトロウェッティング装置を備える電極機構を活性化することを含む、請求項39に記載の方法。
- 前記電極機構を活性化するステップが、光学的に制御される、請求項42に記載の方法。
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US10571475B2 (en) | 2010-12-03 | 2020-02-25 | Cellply S.R.L. | Rapid screening of monoclonal antibodies |
CN102580794B (zh) * | 2011-01-13 | 2014-03-19 | 博奥生物有限公司 | 可定位细胞及生物体的微流控芯片及其应用 |
US9227200B2 (en) | 2011-06-03 | 2016-01-05 | The Regents Of The University Of California | Microfluidic devices with flexible optically transparent electrodes |
EP2739587B1 (en) | 2011-08-01 | 2020-05-27 | Denovo Sciences | Cell capture system |
WO2013148745A1 (en) | 2012-03-28 | 2013-10-03 | Northeastern University | Nanofluidic device for isolating, growing, and characterizing microbial cells |
NZ711723A (en) | 2013-03-28 | 2021-12-24 | Univ British Columbia | Microfluidic devices and methods for use thereof in multicellular assays of secretion |
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