KR102001700B1 - Method for preventing diseases in transplanted rice - Google Patents

Abstract

The present invention provides a method for preventing diseases and / or controlling animal pests in migrant rice plants, which exhibits long-term residual effects in rice paddies, wherein the amount of at least one pesticide is significantly reduced compared to that of conventional seedling box treatments. do. Wherein the composition comprising isothianil is attached to a rice seed before sowing the seed in a seedling box.

Description

METHOD FOR PREVENTING DISEASES IN TRANSPLANTED RICE [0002]

The present invention relates to a method for preventing diseases and / or controlling animal pests in migrant rice.

The treatment of insecticides of rice seeds that are directly related to rice paddies is already known. However, there is no practical seed treatment for the prevention of disease and / or the control of animal pests in rice paddy field rice paddies (Section III: common technique of pesticide application, seed disinfection), Published : 1998, Page: 133-139, edited by Pesticide Science Society of Japan, published by Japan Plant Protection Association).

In addition, although a method of sterilizing an infectious disease of seeds, in which the seed is treated with a seed disinfectant after immersion or after germination, has been described, there is no description about the prevention of diseases or insect pests in rice paddies Publication No. 9224424, Japanese Patent Application Publication No. 11028006, Japanese Patent Application Publication No. 11049612).

For the purpose of preventing diseases and / or controlling animal pests for migrant rice, the pesticides are sprayed in rice paddies or seedling boxes. However, there is a problem in preventing rice disease and / or spraying pesticides on rice paddies for controlling animal pests after migration to rice paddy field, so that a large amount of pesticide is necessary or the effect of pesticide is not long. Also, compared with spraying pesticides on rice paddies, the amount of pesticides can be reduced when pesticides are sprayed on seedling boxes, but in some cases, the amount of pesticides used is still large and the persistence of effects is not sufficient.

It is therefore an object of the present invention to improve these disadvantages and to significantly reduce the amount of pesticides used in comparison with those used in conventional seedling box treatments and to provide a simple and cost- It is intended to provide a method for preventing diseases in rice and / or controlling animal pests.

After a dedicated study to solve the above-mentioned problems, the present inventors have found that by treating the rice seeds with isothianil before sowing the seedling box, the amount of pesticide used is significantly reduced compared with the conventional seedling box treatment, It is possible to realize a sustainable effect. Within the meaning of the present invention, the long-term sustained effect means that disease prevention and / or animal pest control is still observed over 53 days after transition. Preferably, disease prevention and / or animal pest control is still observed at 67 days or more after transplant. Most preferably, the disease prevention and / or animal pest control is still observed over 80 days after the transplant.

On the other hand, in some cases, it has been found that the seeds are treated with other pesticides such as provenazole and thiadinyl, and the harmful effect is brought about. In order to reduce the harmful effect, It has been found that sufficient effects of pesticides can not be obtained after transplanting. The present invention has been completed on the basis of the above findings.

A first embodiment of the present invention relates to a composition comprising isothianil, wherein the rice seeds are attached to rice seeds prior to sowing in a nursery box and the composition comprising isothianil is applied to the rice seeds in an amount of 30 to 180 g and preferably in the range of 40 to 140 g ai / ha, more preferably in the range of 60 to 120 g ai / ha, to prevent disease and / or to control animal pests Method.

An even more preferred embodiment is that the composition comprising isothianil is used in such a manner that the amount of active ingredient is in the range of 40 to 80 g ai / ha, most preferably in the range of 50 to 70 g ai / ha Lt; / RTI >

A second embodiment of the present invention is the above method, wherein the rice seed is after immersion and before germination or after germination.

A third embodiment of the invention is the above process wherein the composition is an aqueous suspension composition comprising isothianil, a surfactant, an organic solvent and a polymeric resin.

A fourth embodiment of the present invention is a method as described above wherein the composition comprises at least one additional pesticide selected from triplexystrobin, penflufen, imidacloprid, thiacloprid, spinosad and etiprol.

A fifth embodiment of the present invention is the method as described above wherein the disease prevention and / or animal pest control is still observed over 53 days after the transition. According to a more preferred embodiment, disease prevention and / or animal pest control is still observed above 67 days after transplant. According to an even more preferred embodiment, the disease prevention and / or animal pest control is still observed more than 80 days after the transplant.

A sixth embodiment of the present invention is the above method wherein the method is for disease prevention.

A seventh embodiment of the present invention is the method as described above, wherein the disease is caused by fungi and / or microorganisms.

An eighth embodiment of the present invention is a method of treating a disease in which the disease is rice blast (caused by Pyricularia oryzae ), rice brown spot or rice head blight ( Due to Cochliobolus miyabeanus ), rice sheath blight (due to Rhizoctonia solani ), rice bacterial leaf blight (due to rice bloom) (Due to Xanthomonas oryzae pv. Oryzae ), and the rice bacterial grain rot (due to Burkholderia glumae ). . ≪ / RTI >

A ninth embodiment of the present invention is the above method wherein the method is for animal pest control.

A tenth embodiment of the present invention is a method for preventing or treating an animal pest, wherein the animal pest is selected from the group consisting of Lissorhoptrus oryzophilus , Oulema oryzae Kuwayama , Delphacidae , Nephotettix cincticeps , Hydrellia griseola (rice leaf miner), Parnara guttata (rice paddy) Rice skipper, Naranga aenescens Moore , Cnaphalocrocis medinalis , Chilo suppressalis , and Suthechaetotlipsuspora , Miss (S tenchaetothrips biformis ) (Rice thrips). ≪ / RTI >

The method of the present invention can reduce the incidence of diseases and / or control animal pests in rice paddy field for a long period of time with a smaller amount of pesticides as compared with the same pesticide used in conventional seedling box treatment as defined above.

The present invention is described in detail below in accordance with embodiments. The entire contents of the references referred to in this specification are intended to be incorporated herein by reference.

The present invention relates to a method for preventing diseases and / or controlling animal pests in rice paddy field rice, characterized in that a composition containing isothianil is attached to rice seeds before sowing in a nursery box.

(3,4-dichloro-2'-cyano-1,2-thiazole-5-carboxanilide (IUPAC name), 3-dichloro-isothiazole- , 4-dichloro- N- (2-cyanophenyl) -5-isothiazole carboxamide (CAS name) are pesticide compounds which have been found to act as toxic emollients, for example herbicide toxicity , WO 2004/103072, WO 2007/057112, WO 2009/124708).

In the method of the present invention, the composition containing isothianil may be in various forms of preparation, for example, a wettable powder, a granular wettable powder, a water-soluble preparation, a liquid preparation, a coatable liquid, an aqueous suspension, Can be listed as. Seed coating formulations may also be used. These preparations can be obtained by known methods, for example, by mixing isothianyl with a developing agent, that is, a liquid or solid diluent or carrier, and in some cases with a surfactant, that is, an emulsifying agent and / or a dispersing agent. When water is used as a developing agent, for example, an organic solvent can be used as an auxiliary solvent. In the present invention, an aqueous suspension is preferable in view of workability. As the aqueous suspending agent, water, a surfactant, a liquid diluent (organic solvent) and a polymer resin, particularly an acrylic resin (a (co) polymer resin containing an alkyl acrylate and / or a methacrylate alkyl ester) Is particularly preferred in view of the persistence of the effect. Antifoaming agents, preservatives, thickeners, dispersants, cryoprotectants and the like may be added to the aqueous suspension composition as required. The preferred isothianyl content in the aqueous suspension composition is 1 to 50 parts by mass, particularly 5 to 40 parts by mass. The preferred surfactant content is 0.01 to 10 parts by mass, particularly 0.1 to 5 parts by mass. The preferred diluent content is 1 to 50 parts by mass, particularly 5 to 30 parts by mass. The preferable content of the acrylic resin is 0.1 to 20 parts by mass, particularly 1 to 10 parts by mass. In the case of other additives, such as antifoaming agents, preservatives and thickeners, the total content is preferably 0 to 10 parts by mass, particularly 0 to 5 parts by mass. As the polymer resin in the aqueous suspension composition, a granular polymer resin is preferable and finely ground by milling or the like is particularly preferable.

Examples of liquid diluents or carriers include aromatic hydrocarbons (e.g., xylene, toluene, alkylnaphthalene, etc.), chlorinated aromatic or chlorinated aliphatic hydrocarbons (e.g., chlorobenzene, ethylene chloride, methylene chloride, etc.), aliphatic hydrocarbons g., cyclohexane or the like, paraffins (e.g. mineral oil fractions etc.)], alcohols (e.g., C _{2 - 10} alcohols, such as butanol, glycol, glycols, such as propylene glycol, and their ethers, esters, etc.) , Ketones (e.g., acetone, methyl ethyl ketone, methyl isobutyl ketone, cyclohexanone, etc.), strongly polar solvents (e.g., dimethyl formamide, dimethyl sulfoxide, etc.), water and the like can be mentioned.

Examples of solid diluents or carriers include crushed natural minerals (e.g., kaolin, clay, talc, chalk, quartz, attapulgite, montmorillonite, diatomaceous earth, etc.), crushed synthetic minerals (e.g. bentonite, silicic acid, alumina, , Silica sand, etc.) and the like can be mentioned. Examples of solid carriers for granular formulations include crushed stone and gypsum (e.g., calcite, marble, pumice, hemihydrate, dolomite, etc.), synthetic granules of inorganic and organic powders, , Coconut shells, corn cobs, tobacco cobs, etc.) can be mentioned.

Examples of surfactants include nonionic and anionic surfactants such as polyoxyethylene fatty acid esters, polyoxyethylene fatty acid alcohol ethers (e.g., alkylaryl polyglycol ethers, alkylsulfonates, alkyl sulfates, Nate), tristyrylphenol and their ethoxylates], albumin hydrolyzate and the like can be mentioned.

Examples of the dispersing agent include lignin-sulfite waste liquid, methyl cellulose and the like.

Adhesives may also be added to the formulations (powder formulations, granulation formulations, emulsions), examples of which include carboxymethylcellulose, natural and synthetic polymers (e.g., gum arabic, polyvinyl alcohol, polyvinyl acetate, etc.) .

Examples of the colorant include inorganic pigments (for example, iron oxide, titanium oxide, prussian blue, etc.) and organic dyes such as alizarin dyes, azo dyes and metal phthalocyanine dyes, and trace elements such as iron , Manganese, boron, copper, cobalt, molybdenum, zinc and the like can be mentioned.

Also, in addition to isothianil, other insecticides such as seed disinfectants, live insecticides and fungicides can be mixed as required. The following combination partners can be listed as an example. These components may be used alone or in combination.

1) Strobilurin fungicide selected from:

Methyloxymethostrobin, orisastrobin, picoxystrobin, paclitaxel, paclitaxel, paclitaxel, paclitaxel, paclitaxel, paclitaxel, paclitaxel, Pyraclostrobin, pyrametostrobin, pyraoxystrobin, and triplexistrobin.

2) SDHI fungicides selected from:

But are not limited to, fosfamily, fosfamily, fosfamily, fosfamily, fosemepiride, isopyrazam, mepronil, oxycarbosine, Pen, pentyopyrid, cedic acid, tefluzamide, benzodifluorpyr and benodanil.

3) neonicotinoid insecticides selected from:

Acetamiprid, chlothianidine, dinotefuran, imidacloprid, niphene pyram, thiacloprid and thiamethoxam.

4) Ryanodine receptor modulator selected from:

Clorantranilylprol, cyaniranylprolol and flubendiamide < RTI ID = 0.0 >

5) Spinosyn Insecticides selected from the following:

Spinosad and Spin Thoram

6) Phenyl pyrazolone insecticides selected from:

Etiprol and fipronil

7) Imidazole-based fungicides selected from:

Methyl-1- (n-butylcarbamoyl) -2-benzimidazole-carbamate (benomyl), 1,2-bis (3-methoxycarbonyl- N-methyl) and pent-4-enyl N-puuoryl-N-imidazol-1-ylcarbonyl-DL-homoalaninate (pefurajoate).

8) Sterol demethylation inhibitors selected from:

(E) -4-chloro-.alpha.,. Alpha.-trifluoro-N- (1- imidazol-1-yl-propoxyethylidene) -o-toluidine (triflumizole) (2- (2,4,6-trichlorophenoxy) ethyl} imidazole-1-carboxamide (prochloraz), 2- Methylethyl) -1- (1H-1,2,4-triazol-1-ylmethyl) cyclopentanol (ipconazole)

9) Copper fungicide selected from:

Copper hydroxide, basic copper chloride, cooper gluconate, and cupperonyl phenol sulfonate.

10) Other selected from the following:

Diethyl-8-oxo [1,3] dioxolo [4,5-g] quinoline-7-carboxylic acid, 4- (2,2-Difluoro-1,3-benzodioxol-4-yl) pyrrole-3-carbonitrile.

Most preferred are the following combinations of pesticides:

Isothianil + trifloxystrobin; Isothianil + penflufen; Isothianyl + imidacurilide; Isothianyl + thiacloprid; Isothianyl + spinosad; Isothianyl + etiprol.

Although there is no particular limitation, the content of isothiamine as an active ingredient is preferably 0.01 to 85% by mass, more preferably 0.1 to 80% by mass, particularly preferably 0.1 to 80% by mass, based on the total amount of the insecticide preparation in view of the amount of the pesticide used in the optimization and treatment of the preparation And preferably in the range of 5 to 70 mass%.

There is no particular limitation on the method of adhering a composition containing isothianyl to rice seeds. As a method of powder coating, for example, rice seed and pesticide are put in a rotary drum and the drum is rotated to uniformly coat rice seeds with pesticides. Examples of the method of spraying the pesticide include (1) a method of spraying the pesticide directly onto the rice seed falling from a hopper or the like using a suitable nozzle, (2) attaching a vibration guide plate from the hopper to the seedling box, (3) a method in which a drum through which rice seeds pass is placed between a hopper and a seedling box, and a method in which rice seeds passing through the drum are sprayed with pesticides And the like. As a method of spraying the pesticide on the rice seeds at a small scale, a method of putting the rice seeds into a rotary machine such as a mortar mixer and spraying the rice paddy seeds uniformly with a pesticide of a predetermined capacity by a suitable atomizer may also be used. Then, the rice seeds are placed in a plastic bag or a suitable container which is 2 to 3 times the rice seed volume, and then the composition containing isothianil is added and closed (in the case of a plastic bag, the inside of the bag is filled with air, And then closing the inlet of the bag), a method of shaking and agitation to cause the pesticide to diffuse into the entire container or bag can also be used. In case of spraying on a large amount of rice seeds, a spray nozzle can be installed at the center of the conveying means for the seedling box to spray the pesticide, and if necessary, the rice seed and the pesticide can be mixed.

According to an additional method of treating rice seeds, pre-germinated rice seeds are treated in a seed processor specifically developed to treat pre-germinated rice seeds instead of dried rice seeds. The seed processor includes a barrel having a predetermined inner diameter, an auger including a shaft for mixing the seed with at least one pesticide, and a flight spirally surrounding the shaft. The auger is housed in a barrel and the tolerance is defined between the auger's flight and the barrel. To treat pre-germinated seeds (here, in the mixing process) without damaging their buds, determine the ratio of tolerance: inner diameter. Preferably, the ratio of the tolerance: barrel inner diameter is set to 1:10.

The seed processor further comprises a tank for containing at least one pesticide and an air compressor for pressurizing and spraying at least one pesticide into the barrel. At least one pesticide may be sprayed into the barrel through at least one spray nozzle (PCT / EP2012 / 058927, unpublished).

The composition containing isothianil is preferably used in a range of 0.1 to 7 g a.i./kg, especially 2 to 4 g a.i./kg in terms of dry seed weight (rice seed weight before immersion). They are preferably used in the range of 0.125 to 10 g a.i./kg, especially 2.5 to 5 g a.i./kg in terms of the seed weight after immersion or in terms of the seed weight after germination. They are in the range of 30 to 180 g ai / ha, preferably in the range of 40 to 140 g ai / ha, more preferably in the range of 60 to 120 g ai / ha in terms of the unit area after migration to rice paddies. , More preferably in the range of 40 to 80 g ai / ha, and most preferably in the range of 50 to 70 g ai / ha.

This is considerably less than the amount of pesticide normally applied to rice seeds.

Regarding rice seeds, it is preferable that the seeds are those after immersion and before germination or after germination. There is also no limitation on the method of immersing the rice seeds, for example, by allowing rice seeds to absorb sufficient water at an integrated temperature (for example, 7 to 10 days at 13 to 14 占 폚) of 60 to 140 占 폚 . After the immersion treatment, the rice seeds may be sown and sprayed with a composition containing isothianil, and then seeded in a seedling box to germinate and grow. When treating rice seeds after germination, for example, rice seeds germinated after germination at 20 to 40 DEG C, preferably at 25 to 35 DEG C, for 10 to 30 hours, preferably 20 to 24 hours, To which a composition containing isothianyl is attached by means of spraying or spraying. After treatment, the rice seeds are sown in a nursery box (for example, 60 cm x 30 cm x 3 cm).

The method of cultivating rice seedlings after sowing rice seeds in a seedling box and covering them with soil, the simulated transplanting in rice paddy field, and the cultivation of rice after transplanting are not particularly limited, and a conventionally known method can be adopted. For example, after covering the seeds with the soil, each seedling box is put into a seedling cabinet to germinate the seeds, and then the seedling box is transferred to the seedling room for further cultivation. It is only necessary to cultivate the seeds cultivated in rice fields by using cultivators. There is no restriction on the growth method of rice after transplanting in rice field, and rice can be cultivated according to a conventional method.

Although the method of the present invention is not particularly limited as to the intended disease, the pesticide may be, for example, Plasmodiophoromycetes, Oomycetes, Zygomycetes, Ascomycetes, Basidiomycetes , ≪ / RTI > Deuteromycetes, and bacteria. In particular, it has been reported that rice bark disease (bakanae disease ( Gibberella , rice blast fungus ( fujikuroi )), rice blast ( Pyricularia oryzae), rice brown spotty disease ( cockroach bolus miyabeanus ), rice sheath blight (due to Liaronia solani ), rice bacterial seedling blight ( Burkholderia plantarii ), rice bacterial leaf blight (Xanthomonas oryzae P. v. Orijera ), rice bacterial egg wilt (Burkholderia glumae), and rice bacterial brown stripe ( Acidovorax < RTI ID = 0.0 > < RTI ID = 0.0 > avenae ). < / RTI > In particular, diseases caused by filamentous fungi and / or microorganisms such as rice blast, rice blotch, rice blast, rice sheath blight, rice bacterial leaf blight, and rice bacterial blight can be effectively prevented .

Within the meaning of the present invention, all rice insect pests can be controlled by the method according to the invention. According to a preferred embodiment of the present invention, the insect pests to be controlled are selected from the group consisting of Lysoltrotus oryzopilus, Oulema Orizaekowayama, Delphacidae, Nepothetics Syntheceps, Hydrelia griseo Manganese beetle), Paranagutta (rice paddy butterfly), Nalangaenesens Moore, Kunapalo Crocis Medinaleis, Chilo Supersalis, and Stenchae Tott Lips Vorportis Is selected.

The present invention will be described based on the following examples.

Example  One

(About 20 parts by weight of isothiene, about 20 parts by weight of a surfactant (which is called a surfactant), 3 g of an active ingredient (isothianyl) per kg of germinated seed obtained by immersing the dried seed in an immersion treatment Propyleneglycol: 10 parts by mass as a diluent, and 5 parts by mass or less in total of additives (defoamer, preservative, thickener, etc.) and the remainder in water The rice seeds were seeded in a plastic bag and mixed well. Next, 150 g of rice seeds (in terms of dry seed weight) were seeded per each seedling box (60 cm x 30 cm x 3 cm) and immediately covered with soil. After covering the seeds with the soil, the seedling box was placed in a seedling cabinet and kept at 30 DEG C for about 3 days. After the emergence, the box was moved to a nursery room, and was vigorously cultivated and cultivated according to a conventional method. The machine was transferred to 20 boxes per 10a (i.e., 20 boxes per 0.1 ha) (about 2.5 leaves). General management such as fertilization was carried out according to the local method.

Example  2

A rice sample was prepared according to the same conditions as in Example 1, except that the same formulation containing 6 g of active ingredient per kg of dry seed was used.

compare Example  One

A rice sample was prepared under the same conditions as in Example 1 except that the isothianyl preparation was not used.

compare Example  2

Rice samples were prepared by applying a formulation (particulate composition containing 2% isothianyl) to the growth box instead of performing the seed treatment. Namely, 2.2 L of seedling seed was poured into a seedling box, 1 L of water was sprayed on the seedling soil, 150 g of seed (in terms of dry seed weight) was then sown, and then 50 g of the above preparation It was sprayed homogeneously and the box was immediately covered with soil. After covering the seeds with the soil, the seedling box was placed in a seedling cabinet and kept at 30 캜 for about 3 days therein. After the emergence, the seedling box was transferred to a seedling room, and the seedlings were planted and cultivated according to a conventional method. The machine was transferred to 20 boxes per 10a (i.e., 20 boxes per 0.1 ha) (about 2.5 leaves). General management such as fertilization was carried out according to the local method.

Examination of disease state

Using the rice samples of Comparative Example 1 (untreated group) as a standard, the disease prevention of rice blast in the rice samples of Example 1, Example 2 and Comparative Example 2 was carried out on days 53, 67 and 80 Day. The value of disease prevention was calculated according to the following formula.

Disease Prevention = 100 - (Occurrence of illness in treated / untreated rice) X 100

Disease outbreak:

0: No leaf lesion

0.2: Approximately 10 leaf lesions (lower leaf type)

0.5: Plant leaf area less than 1%

1: Plant leaf area ratio is 1 to 5%

2: Plant leaf area ratio exceeds 5% and below 10%

3: Leaf area ratio of plants exceeding 10% and below 20%

4: Leaf area ratio of plants exceeding 20% and below 40%

5: Plant leaf area ratio is over 40% and below 80%

6: Plant leaf area exceeds 80%

Based on the test results of disease prevention measures, the disease-preventing effect on rice blast was evaluated by five levels:

0: Preventive value 0 (equivalent to untreated group),

1: Prevention value 1 to 20,

2: Prevention value 21 to 40,

3: Prevention value 41 to 60,

4: Prevention value 61 to 80,

5: Prevention value 81 to 100.

The results are shown in Table 1 (a.i .: active ingredient).

As can be seen from the results in Table 1, the disease-preventing effect of the seed treatment of the rice seed before sowing in the seedling box was only about half (15 ml / kg seed) of the preparation and even after 80 days Showed the same effect as that of the on-sowing treatment, showing that it was very excellent. These results suggest that the pesticide can be more effectively utilized by the method as compared with the conventional treatment method at the time of seeding, and this method is considered to bring about a high disease-preventing effect.

Example  1-2

A rice sample was prepared according to the same conditions as in Example 1, except that the same formulation containing 2 g of active ingredient per kg of dry seed was used.

Example  2-2

A rice sample was prepared according to the same conditions as in Example 1 and the same formulation containing 3 g of active ingredient per kg of dry seed was used.

Example  3-2

A rice sample was prepared according to the same conditions as in Example 1, except that the same formulation containing 4 g of active ingredient per kg of dry seed was used.

Example  4-2

A rice sample was prepared according to the same conditions as in Example 2 and the same formulation containing 6 g of active ingredient per kg of dry seed was used.

compare Example  1-2 and 2-2

All conditions were the same as those of Comparative Examples 1 and 2, respectively.

Examination of disease status for Table 2

Using the rice samples of Comparative Example 1-2 (untreated group) as standard, the rice samples of Examples 1-2, Example 2-2, Example 3-2, Example 4-2, and Comparative Example 2- Prevention of disease of rice blast in 2 rice samples was examined 66 days and 80 days after transplanting. The value of disease prevention was calculated according to the same formula of the above test of disease state.

As can be seen from the results in Table 2, the disease-preventing effect of the seed treatment of the rice seed before sowing in the seedling box was less than 1/3 (10 ml / kg seed) Even in the day, it showed the same effect as the case of the treatment during sowing, showing that it is very excellent. These results suggest that the pesticide can be more effectively utilized by the method as compared with the conventional treatment method at the time of seeding, and this method is considered to bring about a high disease-preventing effect.

Claims (11)

Which is used as a sole active ingredient, is a composition which is adhered to a rice seed before sowing the rice seed in a seedling box, wherein the amount of the active ingredient is in the range of 30 to 180 g ai / ha , A composition for preventing diseases and / or controlling animal pests in rice paddy field rice. The composition according to claim 1, wherein the amount of active ingredient is in the range of 40 to 140 g ai / ha. 3. The composition according to claim 1 or 2, wherein the rice seed is after immersion and before germination or after germination. The composition of claim 1 or 2, wherein the composition is an aqueous suspension comprising isothianil, a surfactant, an organic solvent and a polymeric resin. 3. The composition according to claim 1 or 2, wherein the disease prevention and / or animal pest control is still observed above 53 days after transplant. The composition according to claim 1 or 2, wherein the composition is for preventing disease. 3. The composition according to claim 1 or 2, wherein the disease is caused by filamentous fungi and / or microorganisms. The method according to claim 1 or 2, wherein said disease is rice blast (caused by Pyricularia oryzae ), rice brown spot or rice head blight ( Due to Cochliobolus miyabeanus ), rice sheath blight (due to Rhizoctonia solani ), rice bacterial leaf blight (Due to Xanthomonas oryzae pv. Oryzae ), and rice bacterial grain rot (due to Burkholderia glumae ) ≪ / RTI > 3. The composition according to claim 1 or 2, wherein the composition is for controlling animal pests. 3. The method according to claim 1 or 2, wherein the animal pest is selected from the group consisting of Lissorhoptrus oryzophilus , Oulema oryzae Kuwayama , Delphacidae , Nephotettix cincticeps , Hydrellia griseola (rice leaf miner), Parnara guttata (rice paddy) Rice skipper, Naranga aenescens Moore , Cnaphalocrocis medinalis , Chilo suppressalis , and Suthechaetotlipsuspora , Miss (S tenchaetothrips biformis ) (Rice thrips). ≪ / RTI > delete