JP2011517679A - How to reduce chemical damage - Google Patents

Abstract

  A method of reducing phytotoxicity of a seed crop by treating seeds with 3,4-dichloro-isothiazole-5-carboxylic acid-2-cyanoanilide before sowing.

Description

  The present invention relates to a method for reducing phytotoxicity. Specifically, the present invention relates to a method for reducing phytotoxicity by seed treatment with a specific drug.

  It is already known that the specific isothiazole carboxamides described in JP2001522840T have a phytotoxicity-reducing action (see JP200004346030A). Furthermore, 3,4-dichloro-isothiazole-5-carboxylic acid-2-cyanoanilide (generic name isothianyl) is sprayed in advance in a nursery box for the purpose of reducing paddy rice phytotoxicity caused by herbicides in Honda after transplanting rice. It is known that this method is effective (see JP2007137833A).

JP-T-2001-522840 JP 2004-346030 A JP 2007-137833 A

  In recent years, labor-saving and low-cost rice cultivation has been demanded, and there is a great expectation for establishment of direct sowing cultivation technology that greatly reduces spring work such as seedling production compared to transplant cultivation. However, in general, in direct sowing, there is a tendency that the phytotoxicity by the herbicide tends to be strong, and it is desired to be solved immediately.

  The present inventors have found that by treating seeds with isothianyl, in Honda, for example, a clear mitigating effect is exhibited against phytotoxicity caused by herbicide treatment, and the present invention has been completed. The present invention is also applicable to transplant cultivation. That is, by raising seedlings after seed treatment with isothianyl, it also exerts a mitigation effect on drug damage caused by drug treatment during transplanting and after transplanting to Honda, for example, herbicide treatment.

The herbicidally active compound that is the target of the reduction in phytotoxicity that can be achieved by using the method of the present invention is not particularly limited, but examples thereof include the following.
As an acetyl CoA carboxylase (ACCase) inhibitor,
Aryloxyphenoxypropionic acid-based ACCase inhibitors: Clodinahop propargyl, cihalohop butyl, diclohop methyl, phenoxaprop P ethyl, fluazifop P butyl, halokihop R methyl, propoxahop, quizalofop P Ethyl, metamihop,
Cyclohexanedione-based ACCase inhibitors: alloxidim, butroxidim, cresodymium, cycloxydim, profoxydim, cetoxydim, teplaloxidim, tralcoxidim,
Phenylpyrazoline-based ACCase inhibitor: Pinoxaden.
As an acetolactate synthase (ALS) inhibitor,
Sulfonylurea-based ALS inhibitors: amidosulfuron, azimusulfuron, bensulfuron methyl, chlorimuron ethyl, chlorsulfuron, synosulfuron, cyclosulfamuron, ethamethsulfuron methyl, ethoxysulfuron, frazasulfuron, flupirsulfuron・ Methyl ・ Na, foramsulfuron, halosulfuron / methyl, imazosulfuron, iodosulfuron, mesosulfuron / methyl, metsulfuron / methyl, nicosulfuron, oxasulfuron, primsulfuron / methyl, pyrazosulfuron / ethyl, rimsulfuron, sulfometuron ・Methyl, sulfosulfuron, thifensulfuron methyl, triasulfuron, tribenuron methyl, trifloxysulfuron, triflusulfuron methyl, tri Surufuron, ortho Sul Pham Ron, TH547, NC620,
Imidazolinone (ALS) inhibitors: imazapic, imazametabenz methyl, imazamox, imazapill, imazaquin, imazetapill,
Triazolopyrimidine-based ALS inhibitors: chloranthran slam methyl, diclos ram, flurosum ram, flume slam, methoslam, penox slam,
Pyrimidinyl salicylic acid ALS inhibitors: bispyribac sodium salt, pyribenzoxime, pyriftalide, pyrithiobac sodium salt, pyriminobac methyl, pyrimisulfurphan,
Triazolinone-based ALS inhibitors: Flucarbazone sodium salt, propoxycarbazone sodium salt, thiencarbazone,
As a photosynthesis inhibitor (photochemical system II),
Triazines: amethrin, atrazine, cyanazine, desmethrin, dimetamethrin, prometone, promethrin, propazine, simazine, cimethrin, terbumethone, terbutyrazine, terbutrin, trietadine,
Triazinone series: hexazinone, metamitron, metribuzin,
Triazolinone series: amicarbazone,
Uracil: Bromacil, Lenacil, Turbacil,
Pyridazinone series: Chloridazon,
Phenyl carbamates: Death Medifum, FenMedifum,
Urea: Chlorbromulone, chlorotoluron, chloroxuron, dimeflon, diuron, etizimuron, phenuron, fluometuron, isoproturon, isouron, linuron, metabenzthiazuron, metblomron, methoxuron, monolinuron, nebulon, ciduron, tebuthiuron,
Amide series: propanyl, pentanochlor,
Nitriles: bromophenoxime, bromoxynyl, ioxynil,
Benzothiadiazinones: Bentazone,
Phenylpyridazine series: pyridate, pyridafor,
As a toxic agent (photochemical system II) by photoactivation,
Bipyridinium series: Diquat, Paraquat,
As a protoporphyrinogen oxidase (PPO) inhibitor,
Diphenyl ether system: acifluorfen, bifenox, clomethoxyphen, fluoroglycophene, fomesafen, halosafene, lactofen, oxyfluorfen, clomethoxynil,
Phenylpyrazole series: fluazolate, pyraflufen / ethyl,
N-phenylphthalimide series: sinidone ethyl, flumioxazin, full microlac pentyl,
Thiadiazole series: Fruthiaset methyl, thiazimine,
Oxadiazole series: oxadiazone, oxadiargyl,
Triazolinone series: azaphenidine, carfentrazone ethyl, sulfentrazone,
Oxazolidinedione series: pentoxazone,
Pyrimidinedione series: benzphendizone, butaphenacyl,
Others: Pyraclonil, profluazole, flufenpyr ethyl,
As a carotenoid biosynthesis inhibitor,
1, PDS inhibitor pyridazinone series: norflurazon,
Pyridine carboxamides: diflufenican, picolinaphen,
Others: beflubutamide, fluridone, flurochloridone, flurtamon,
2,4-HPPD inhibitor Triketone series: mesotrione, sulcotrione, benzobicyclone, tefryltrione,
Isoxazole series: isoxachloritol, isoxaflutole,
Pyrazole series: benzophenap, pyrazolinate, pyrazoxifene,
Other: benzobicyclone,
3. Unexplained target Triazole: Amitrol,
Isoxazolidinone series: clomazone,
Diphenyl ether type: acronifene,
As an EPSP synthase inhibitor,
Glycine system: glyphosate, glyphosate trimesium salt,
As a glutamine synthetase inhibitor,
Phosphinic acid series: glufosinate, bialaphos,
As a DHP biosynthesis inhibitor,
Carbamate: Ashram,
As a microtubule polymerization inhibitor,
Dinitroaniline series: bethrosin, butorualine, dinitramine, ethalfluralin, oryzalin, pendimethalin, trifluralin,
Phosphoric acid amide system: Amiprophos methyl, butamifos,
Pyridine series: dithiopyr, thiazopyr,
Benzamide series: Propizzamid, Tebutam, Chlortal dimethyl,
As an inhibitor of mitosis / microtubule formation,
Carbamates: Chlorprofam, Profam, Carbetamid,
As an inhibitor of super long chain fatty acid biosynthesis,
Chloroacetamide series: acetochlor, alachlor, butachlor, dimetachlor, dimethenamide, metazachlor, metolachlor, petoxamide, pretilachlor, propachlor, propisochlor, tenyl chlor,
Acetamide series: diphenamide, napropamide, naproanilide,
Oxyacetamide series: full phenacet, mefenacet,
Tetrazolinone: phentolazamide,
Others: Anilophos, Cafétrol, Piperofos,
As a cellulose biosynthesis inhibitor,
Nitrile type: Niclobenil, Chlorthiamide,
Benzamide series: isoxaben,
Triazolocarboxamide series: Flupoxam,
Quinoline carboxylic acid type: quinchlorac,
As an uncoupler,
Dinitrophenol type: DNOC, dinocebu, dinoterb,
As a fatty acid elongation inhibitor (non-ACCase inhibition),
Thiocarbamate series: butyrate, cycloate, dimethylpiperate, EPTC, esprocarb, molinate, olvencarb, pebrate, prosulfocarb, beniocarb, pyributycarb, thiocarbazyl, triarate, vernarate,
Phosphoric acid dithioate type: bensulfuride,
Benzofuran: Benfresate, Etofumesate,
Chlorocarbonic acid series: TCA, Dalapon, Tetrapion,
As an auxin-like herbicide,
Phenoxycarboxylic acid type: Chlomeprop, 2,4-D, 2,4-DB, Dicloprop, MCPA, MCPB, MCPP,
Benzoic acid series: chloramben, dicamba, 2,3,6-TBA,
Pyridinecarboxylic acid series: clopyralide, fluroxypyr, picloram, triclopyr, quinclolac, kimmelac,
Other: Benazoline ethyl,
As an auxin translocation inhibitor,
Naphthalamate series: Napthalam
Semicarbazone series: diflufenzopyr sodium salt,
Others (unknown mechanism of action)
Arylaminopropionic acid series: flamprop / M / methyl, flamprop / isopropyl,
Pyrazolium system: Difenzo coat,
Organic arsenic: DSMA, MSMA,
Others: bromobutide, chlorflurenol, symmetrine, cumyluron, dazomet, diimron, methyldaimlone, ettobenzanide, fosamine, indanophan, metam, oxadichromemephone, oleic acid, pelargonic acid, pyributycarb.

  These herbicidal compounds (described by generic names or derived names) are for example mainly described in The Pesticide Manual, 14th edition (British Crop Protection Council publication 2007) or are already well known.

  By the method of the present invention, a selective herbicidal effect between cultivated plants and weeds can be obtained. As used herein, weed means all plants that grow in undesirable places in a broad sense. For example, the following weeds and cultivated plants can be used.

  Genus of dicotyledonous weeds: Sinapis, Capsella, Leipidium, Galium, Stellaria, Chenopodia, T , Sangcio, Bob, Amaranthus, Amaranthus, Portulaca, Xanthium, Ipomoea, Polygaum, Polygum ), Nochusi (Sonchus), Solanum potato (Solanum), Inugarashi (Rorip) a), Laver, Veronica, Datura, Violet, Galeopsis, Papaver, Centaurea, Centaurea Rotala, Azena (Lindernia), American horned phoenix (Sesbania), White clover (Trifolium), Ibibi (Abutilon), Lamumium, Matricia (America), Japanese mugwort (Arte) Pharbitis) et al.

  The genus of dicotyledonous plants: cotton (Gossypium), soybean (Glycine), chard sugar beet (Beta), carrot (Daucus), common bean (Peanus), pea (Pisum), eggplant potato (Solanum) (Linum), sweet potato, morning glory (Ipomoea), broad bean, nantenhagi (Vicia), tobacco (Nicotiana), tomato (Lycopersicon), peanut (Arachis), rape, cabbage cabbage (Brassica), acne -Melon (Cucumis), pumpkin (Cucurbita), etc.

  The genus of monocotyledonous weeds: Echinochlona, Echocora awa (Setaria), millet (Panicum), tiger beetle (Digitalia), awagarie timothy (Phlum), strawberries Ohishiba Shikokubie (Eleuusine), Dokumugi (Lolium), Swan-growth Inumugi (Bromus), Oats oats (Ovena) (Avena), Cyperus papyrus shichitoui hamasuge (Cyper), Moro-g Konogi (Monochoria), Tentsuki (Fimbristylis), Omodaka Kwai (Sagittaria), Harii Logei (Eleocharis), Firefly Ukiyagura Futui (Scirpus), Vulgaris (Paspalum), Platypus (Ischaeum), Nukabo (Agrostis), Vulgaris (Alopecurus), Ginotica (Cynocus) (Leptochloa) et al.

  Genus of monocotyledonous plants: rice (Oryza), corn hop corn (Zea), wheat (Triticum), barley (Hordeum), oats (Ovena), rye (Secale), sorghum (Sorghum), Millet (Panicum), sugar cane, Waseobana (Saccharum), banana (Musa), pineapple (Ananas), asparagus (Asparagus), leek and leek (Allium) and the like.

  Furthermore, the said method of this invention is not limited to the above-mentioned plant, It can apply similarly to another plant.

  Furthermore, the method of the present invention can be used to control weeds in perennial plant cultivation, such as afforestation, ornamental orchards, orchards, vineyards, citrus fields, nut gardens, banana gardens, coffee gardens. It can be applied to tea plantations, rubber gardens, oil palm gardens, cocoa gardens, soft fruit gardens, hop fields and the like. Furthermore, this method can be applied to selective weed control in annual plant cultivation.

  The method of the present invention can obtain a selective weeding effect between paddy rice and paddy weeds. Examples of paddy weeds that can be controlled include the following.

  Dicotyledonous plants of the following genera: Polygonum, Roppa, Rubila, Lindena, Binders, Dopatrium, Esprit The genus Elatine, Gratiola, Linderia, Ludwigia, Oenanthe, Ranunculus, and Destros.

  Monocotyledonous plants of the following genera: Echinochloa, Millic genus (Panicum), Papaver genus (Poa), Cyperus genus (Monperoria), Monochoria genus (Fimbristiris), Sidum genus (S) Hario (Eleocharis), Firefly (Sirpus), Sasiomodaka (Alisma), Ibelia (Aneilema), Subuta (Blyxa), Hossa (Eriocaulon), Potatobetia (Potamogeta), (Leptochloa), Sphenocrea, etc.

More specifically, for example, it can be used for the following representative paddy weeds.
Plant name Latin name Dicotyledonous vegetative plant Marisilea quadrifolia
HIDERIKO FIBRISTYLIS Miliacea
Nagano Nourushi Sphenoclea zeylanica
Himemisohagi Ammannia sp.
Kita Shigusa Rotala indica Koehne
Azena Linderia procumbens Philcox
Aoubiru Amaranthus viridis
American Azena Lindernia dubia L. Penn.
Takasaburo Eclipse prostrata
Azeto pepper Lindernia angustifolia
Ludwigia prostrate Roxburgh
Polygonum Polygonum sp.
American Tsunoxanem Sesbania exaltata
Hiramshiro Potamogeton distinctus A. Benn
Bean morning glory Ipomoea lakunosa
Mizokokobe Elatin triandra Schk
Seri Oenanthe javanica
Monocotyledonous Einochloa oryzicola Vasing
Kochimebie Echinochlor colonum
Inuville crus-galli
Ishieum rugosum
Millet Panicum sp.
Ohashiba Eleusine indica
Bark beetle Digitalia sp.
Kishus sumenopas Paspalum disticum
Cyperus iria
Hamasuge C.I. rotundus
Matsubai Eleocharis acicularis L.
Crogwai Eleochris kuroguwai Ohwi
Tamagayatsuri Cyperus differentials L.
Cyprinus serotinus Rottboel
Shirapus mucronatus
Koukiyagara pniculmis
Firefly Sirpus juncoides Roxburgh
Konagi Monochoria vaginalis Presl
Urigawa Sagitaria pygmaea Miq
Heramodaka Alisma canalicatum A. Br. et Bouche
Sagiomodaka plantago-aquatica
Omodaka Sagittaria trifolia
Mizoaoi Monochoria korsakowii
Acne Brachiaria plantaginea
Azegaya Leptochloa chinensis

  The method of the present invention is not limited to the use of these grass species on weeds, and can be similarly applied to other grass species of weeds.

  In practicing the method of the present invention, the seed is treated with a composition containing isotianil in a sprouting or non-sprouting state before sowing the seed in a nursery box, Honda or upland. Specifically, for example, immersion treatment or dressing treatment can be performed, thereby reducing phytotoxicity at the time of breeding in a seedling box and Honda after transplanting, Honda after direct seeding, or upland.

  The method of treatment according to the invention is used for the treatment of genetically engineered organisms (GMO), for example plants or seeds. A genetically modified plant (or transgenic plant) is a plant in which a heterologous gene is stably integrated into the genome. The expression “heterologous gene” is a gene supplied or constructed outside a plant that, when introduced into the nucleus, chloroplast or mitochondrial genome, expresses a protein or polypeptide of interest, or In transformed plants by down-regulating or silencing other gene (s) present in the plant (eg, using antisense technology, co-suppression technology or RNA interference-RNAi-technology) Essentially means a gene that confers new or improved agronomic or other properties. A heterologous gene that is placed in the genome is also called a transgene. A transgene that is designated at a particular location in the plant genome is called a transformation or gene transfer event.

  Depending on the type of plant or plant cultivar, their location and growth conditions (soil, climate, growing season, nutrients), the treatment according to the invention can also produce a superaddition (“synergistic”) effect. Thus, for example, for active compounds and compositions that can be used according to the invention, reduced application rates and / or broadening of the spectrum of activity and / or increased activity, better plant growth, increased resistance to high or low temperatures Increased resistance to drought or water damage or soil salinity, increased flowering performance, easier harvesting, accelerated maturation, higher yield, larger fruit, larger plant height, greener leaf color of harvested product, Faster flowering, higher quality and / or higher nutritional value, higher sugar concentration in the fruit, better storage stability and / or processability of the harvested product is possible, which is actually the expected effect It exceeds.

  Plants and plant cultivars that are preferably treated according to the present invention are plants with genetic material that give these plants particularly advantageous and useful traits (even if obtained by breeding and / or biotechnological means). Including all.

  Plants and plant cultivars that are also preferably treated according to the present invention are resistant to one or more biological stresses. That is, the plant exhibits better protection against animal and microbial pests, such as nematodes, reptiles, ticks, phytopathogenic fungi, bacteria, viruses and / or viroids.

  Plants and plant cultivars that can also be treated according to the present invention are plants that are resistant to one or more abiotic stresses. Abiotic stress conditions include, for example, drought, low temperature exposure, heat exposure, osmotic stress, flooding, increased soil salinity, increased mineral exposure, ozone exposure, high light exposure, nitrogen nutrient availability constraints, May include restrictions on availability of phosphorus nutrients, shade avoidance.

  Plants and plant cultivars that can also be treated according to the invention are plants characterized by enhanced harvest characteristics. High yields of the plant include improved plant physiology, growth and growth, for example, water use efficiency, water retention efficiency, improved nitrogen use, enhanced carbon assimilation, improved photosynthesis, high germination efficiency and accelerated maturation. Result. Yield may be further affected by improved plant architecture (under stress and non-stress conditions), which includes early flowering, flowering control for hybrid seed production, seedling vigor, plant dimensions, Internode number and spacing, root growth, seed size, fruit size, pod size, number of pods or ears, number of seeds per pod or ear, seed mass, increased seed filling, reduced seed scattering, pod dehiscence and lodging resistance Including, but not limited to, a reduction in sex. Additional harvest traits include carbohydrate content, protein content, fat content and composition, nutritive value, seed composition such as reduced anti-nutritive compounds, improved processability and better storage stability.

  Plants that can be treated according to the present invention are hybrid plants that already exhibit the characteristics of heterosis or hybrid stress that generally results in higher yields, vitality, health and resistance to biological and abiotic stressors. This type of plant is usually made by crossing one inbred male sterile parent line (female parent) with another inbred male sterile parent line (male parent). Hybrid seed is usually harvested from male sterile plants and sold to growers. Male sterility plants can sometimes be produced (eg, in corn) by ear removal, ie, mechanical removal of the male genitalia (or male flower), but more typically male sterility is It is the result of genetic determinants. In that case, and especially when the seed is the desired product harvested from the hybrid plant, it is usually useful to ensure that the male fertility of the hybrid plant is fully restored. This is accomplished by ensuring that the male parent has an appropriate fertility-recovery gene that can restore male fertility to the hybrid plant containing the genetic determinant responsible for male sterility. can do. Male sterility genetic determinants may be placed in the cytoplasm. Examples of cytoplasmic male sterility (CMS) have been described for example in Brassica species. However, male sterile genetic determinants can also be placed in the nuclear genome. Male sterile plants can also be obtained by plant biotechnological methods such as genetic engineering. A particularly useful means of obtaining male sterile plants is described in WO 89/10396, for example, ribonucleases such as barnase are selectively expressed in stamen carpet tissue cells. Fertility can then be restored by expression in carpet tissue cells of a ribonuclease inhibitor such as barster.

  Plants or plant cultivars that can be treated according to the present invention (obtained by plant biotechnological methods such as genetic engineering) are resistant to herbicide resistant plants (ie to one or more given herbicides). Plant). This type of plant can be obtained by selecting plants containing genetic transformations or mutations that confer resistance to this type of herbicide.

  Herbicide tolerant plants are, for example, glyphosate tolerant plants, i.e. plants that have become tolerant to the herbicide glyphosate or salts thereof. Plants can be made resistant to glyphosate by different means. For example, glyphosate-tolerant plants can be obtained by transforming plants having a gene encoding 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Examples of this kind of EPSPS gene are the AroA gene (mutant CT7) of the bacteria Salmonella typhimurium, the CP4 gene of the genus Agrobacterium sp., Petunia EPSPS, tomato EPSPS or It is a gene encoding Eleusine EPSPS. It may also be a mutated EPSPS. Glyphosate-tolerant plants can also be obtained by expression of a gene encoding glyphosate oxidoreductase. Glyphosate-tolerant plants can also be obtained by expression of a gene encoding glyphosate acetyltransferase. Glyphosate-tolerant plants can also be obtained by selecting plants that contain naturally occurring mutations of the aforementioned genes.

  Other herbicide-tolerant plants are plants that become resistant to herbicides that inhibit the enzyme glutamine synthase such as bialaphos, phosphinotricin or glufosinate, for example. This type of plant can be obtained by expression of an enzyme that detoxifies the herbicide or a mutant glutamine synthase that resists inhibition. One such efficient detoxifying enzyme is an enzyme encoding a phosphinothricin acetyltransferase (such as a bar or pat protein from Streptomyces species). Plants that express exogenous phosphinotricin acetyltransferase have also been described.

  Further herbicide-tolerant plants are plants that become resistant to herbicides that inhibit the enzyme hydroxyphenylpyruvate dioxygenase (HPPD). Hydroxyphenylpyruvate dioxygenase is an enzyme that catalyzes a reaction in which para-hydroxyphenylpyruvate (HPP) is converted to homogentisic acid. Plants resistant to HPPD inhibitors can be transformed with a gene encoding a naturally occurring resistant HPPD enzyme or a gene encoding a mutated HPPD enzyme. Resistance to HPPD inhibitors is also obtained by transforming plants with genes encoding certain enzymes that allow the formation of homogentisic acid despite the inhibition of the native HPPD enzyme by HPPD inhibitors. be able to. Plant tolerance to HPPD inhibitors can also be improved by transforming plants with a gene encoding the enzyme prephenate dehydrogenase in addition to the gene encoding the HPPD resistant enzyme.

  Still further herbicide-resistant plants are plants that become resistant to acetolactate synthase (ALS) inhibitors. Known ALS inhibitors include, for example, sulfonylureas, imidazolinones, triazolopyrimidines, pyrimidinyloxy (thio) benzoates and / or sulfonylaminocarbonyltriazolinone herbicides. Various mutations in the ALS enzyme (also known as acetohydroxyacid synthase, AHAS) are known to confer resistance to various herbicides and herbicide groups. Production of sulfonylurea resistant plants and imidazolinone resistant plants has been described. Other imidazolinone resistant plants have also been described. Further sulfonylurea and imidazolinone resistant plants are also described, for example, in WO 2007/024782.

  Other plants that are resistant to imidazolinones and / or sulfonylureas are induced by mutations in the presence of herbicides, by selection in cell culture, or, for example, in soybean, rice, sugar beet, lettuce, or sunflower. It can be obtained by the mutation breeding described.

  Plants or plant cultivars that can be treated according to the present invention (obtained by plant biotechnological methods such as genetic engineering) are resistant to attack by insect-resistant transgenic plants, ie specific target reptiles. A plant that has become resistant. Such plants can be obtained by genetic transformation or by selecting plants containing mutations that confer this type of insect resistance.

“Insect resistant transgenic plant” as used herein includes any plant comprising at least one of the following transgenes, including coding sequence encoding:
1) Insecticidal crystal protein from Bacillus thuringiensis or an insecticidal part thereof, such as Crickmore et al. Microbiology and Molecular Biology Reviews (1998), 62, 807-813, et al. -Bacillus thuringiensis toxin nomenclature, online: http: // www. lifesci. sussex. ac. an insecticidal crystal protein or an insecticidal part thereof as updated by uk / Home / Neil_Crickmore / Bt /, for example a protein of the Cry proteins Cry1Ab, Cry1Ac, Cry1F, Cry2Ab, Cry3Aa or Cry3Bb; or an insecticidal part thereof; or 2) Cry34 Bacillus thuringii, which is insecticidal in the presence of a second other crystal protein from Bacillus thuringiensis or part thereof, such as a binary toxin composed of Cry35 crystal protein and Cry35 crystal protein Crystal protein from Bacillus thuringiensis or part thereof; or 3) Bacillus thuringiensis Cry1A produced by a hybrid insecticidal protein, such as corn event MON98034 that include portions of various insecticidal crystal proteins from. A hybrid of the protein of 1) or a hybrid of the protein of 2), such as 105 protein; or 4) a protein of any one of 1) to 3), wherein the insecticidal power is higher against the target worm species And / or during the cloning or transformation, such as Cry3Bb1 protein of corn event MON863 or MON88017, or Cry3A protein of corn event MIR604 Due to changes introduced in the encoded DNA in some proteins, in particular from 1 to 10 amino acids have been replaced with other amino acids;
5) http: // www. lifesci. sussex. ac. uk / home / Neil_Crickmore / Bt / vip. plant insecticidal proteins (VIP) listed in html, for example, insecticidal secreted proteins from Bacillus thuringiensis or Bacillus cereus, such as proteins from the VIP3Aa protein class, Or an insecticidal part thereof; or 6) Bacillus thuringiensis or B., such as a binary toxin composed of VIP1A and VIP2A proteins. A protein secreted from Bacillus thuringiensis or Bacillus cereus that is insecticidal in the presence of a second protein secreted from B. cereus; or 7) above A hybrid insecticidal protein comprising portions from various secreted proteins from Bacillus thuringiensis or Bacillus cereus, such as a hybrid of 1) or a hybrid of 2) above; Or 8) the protein of any one of 1) to 3) above for obtaining and / or affecting a higher insecticidal power against the target species In order to expand the range of reptile species and / or changes introduced into the encoded DNA during cloning or transformation (while encoding the insecticidal protein), such as the VIP3Aa protein of cotton event COT102 For this reason, some proteins, in particular 1 to 10 amino acids, have been replaced with other amino acids.

  Of course, insect-resistant transgenic plants, as used herein, also include any plant that contains a combination of genes that encode any one protein of types 1 to 8 above. In one embodiment, the insect-resistant plant is used to expand the range of target reptile species that are affected when using different proteins directed against different target reptile species, or to the same target reptile species. To slow the development of insect resistance to plants by using various proteins that are insecticidal but have different modes of action (such as binding to various receptor binding sites of reptiles) A plurality of transgenes encoding any one protein of types 1 to 8 above.

Plants or plant cultivars (obtained by plant biotechnological methods such as genetic engineering) that can also be treated according to the present invention are resistant to abiotic stress. Such plants can be obtained by genetic transformation or by selection of plants containing mutations that confer this type of stress resistance. Particularly useful stress tolerant plants include:
a. A plant comprising a transgene capable of reducing the expression and / or activity of a plant cell or plant poly (ADP-ribose) polymerase (PARP) gene b. A plant comprising stress tolerance that enhances a transgene capable of reducing the expression and / or activity of PARG encoding the gene of the plant or plant cell c. Transgenes encoding plant functional enzymes of the nicotinamide adenine dinucleotide salvage synthesis pathway including nicotinamidase, nicotinic acid phosphoribosyltransferase, nicotinic acid mononucleotide adenylyltransferase, nicotinamide adenine dinucleotide synthase or nicotinamide phosphoribosyltransferase Plants containing stress tolerance to enhance.

  Examples of plants having the aforementioned traits are listed in Table A, but are not limited to these.

Plants or plant cultivars (obtained by plant biotechnological methods such as genetic engineering) that can also be treated according to the present invention are modified amounts, quality and / or storage stability of the harvested product, such as: And / or exhibit altered properties of certain components of the harvested product.
1) Physicochemical properties, especially amylose content or amylose / amylopectin ratio, degree of branching, average chain length, side chain distribution, viscous behavior, gel strength, starch granule size and / or so as to be more suitable for special applications Alternatively, transgenic plants that synthesize modified starches that are altered in starch grain form compared to starch synthesized in wild-type plant cells or plants.
2) A transgenic plant that synthesizes a non-starch carbohydrate polymer or synthesizes a non-starch carbohydrate polymer that has modified properties compared to a wild type plant without genetic changes. Examples include, among others, plants that produce inulin and levan-type polyfructose, plants that produce alpha-1,4-glucan, produce alpha-1,6-branched alpha-1,4-glucan There are plants that produce alternan.
3) A transgenic plant producing hyaluronan.

  A particularly useful transgenic plant that can be treated according to the present invention is the subject of a petition to the United States Department of Agriculture (USDA) Animal and Plant Quarantine Service (APHIS) seeking to exclude restrictions within the United States. Plants containing transformation events or combinations of transformation events that are permitted or pending. At any time, this information is readily available from APHIS (4700 River Road Riverdale, MD 20737, USA), such as its Internet site (URL http://www.aphis.usda.gov/brs/not_reg.html). The petitions pending APHIS or granted by APHIS for the exclusion of regulations were listed in Table B containing the following information as of the filing date of this application.

Petition: Petition identification number. A professional description of the transformation event can be found in the individual petition documents available from the APHIS, eg the APHIS website with reference to this petition number. These descriptions are incorporated herein by reference.
Petition extension: A reference to a previous petition for which an extension is required.
Institution: Name of the entity submitting the petition.
Controlled goods: Plant species involved.
Genetically modified phenotype: A trait conferred to a plant by a transformation event.
Transformation event or strain: The name (s) of the event for which exclusion is sought (sometimes also referred to as strain (s)).
APHIS Documents: Various documents published by APHIS regarding petition and that can be requested from APHIS.

  In the method of the present invention, isotianil can be formulated into various preparation forms. Specific examples thereof include a wettable powder, a granular wettable powder, an aqueous solvent, a liquid, an AL preparation, an aqueous suspension. Agents, microcapsules and the like can be exemplified. Furthermore, seed coating preparations can also be used. These formulations are known per se, for example by mixing isotianil with a developing agent, ie a liquid or solid diluent or carrier, and optionally with a surfactant, ie an emulsifier and / or a dispersant. Is done by. In this case, when water is used as a developing agent, for example, an organic solvent can be used as an auxiliary solvent.

  Liquid diluents or carriers include, for example, aromatic hydrocarbons (eg, xylene, toluene, alkylnaphthalene, etc.), chlorinated aromatic or chlorinated aliphatic hydrocarbons (eg, chlorobenzenes, ethylene chloride, methylene chloride) Etc.), aliphatic hydrocarbons [for example, cyclohexane, paraffins (for example, mineral oil fraction, etc.)], alcohols (for example, butanol, glycol, etc. and their ethers, esters, etc.), ketones (for example, acetone, Methyl ethyl ketone, methyl isobutyl ketone, cyclohexanone, etc.), strong polar solvents (eg, dimethylformamide, dimethyl sulfoxide, etc.), water and the like.

  Solid diluents or carriers include, for example, ground natural minerals (eg, kaolin, clay, talc, chalk, quartz, attapul guide, montmorillonite, diatomaceous earth, etc.), ground synthetic minerals (eg, highly dispersed silicic acid, alumina, silicic acid) Salt, etc.). Solid carriers for granules include crushed and separated rocks (eg, calcite, marble, pumice, foamstone, dolomite, etc.), synthetic grains of inorganic and organic powders, organic substances (eg sawdust, coconut palm) Corn, corn cobs, tobacco stems, etc.).

  Surfactants include, for example, nonionic and anionic surfactants [eg, polyoxyethylene fatty acid esters, polyoxyethylene fatty acid alcohol ethers (eg, alkylaryl polyglycol ethers, alkyl sulfonates, alkyl sulfates, aryl sulfates). Sulfonates)], albumin hydrolysis products, and the like.

  Dispersants include, for example, lignin sulfite waste liquor and methylcellulose.

  Adhesives can also be used in preparations (powder, granules, emulsions), such as carboxymethylcellulose, natural and synthetic polymers (eg gum arabic, polyvinyl alcohol, polyvinyl acetate, etc.). Can do.

  A colorant can also be used. Examples of the colorant include inorganic pigments (eg, iron oxide, titanium oxide, Prussian blue, etc.), alizarin dyes, organic dyes such as azo dyes or metal phthalocyanine dyes, and A trace element such as a salt of a metal such as iron, manganese, boron, copper, cobalt, molybdenum, or zinc can be used.

  The preparation can generally contain isothianyl in the range of 0.01 to 95% by weight, preferably 0.1 to 90% by weight.

  In the seed treatment, 0.001 g to 50 g, preferably 0.01 g to 10 g of isotianil can be used per 1 kg of seed.

  Next, although the specific example shows the manufacture and use of the compound of the present invention by the following examples, the present invention should not be limited to this.

Biological tests and formulation examples Test compounds 3,4-Dichloro-isothiazole-5-carboxylic acid-2-cyanoanilide (generic name: isothianyl)
Herbicide (generic name)
H-1: Phenoxaprop / P / ethyl H-2: Oxadiargyl (Preparation of reagent)
Carrier: Acetone 5 parts by weight Surfactant: Benzyloxypolyglycol ether 1 part by weight The above carrier and surfactant and 1 part by weight of isotianil are mixed, and the resulting preparation is diluted with water to give a predetermined amount of isothianyl. An aqueous suspension is prepared.

  Seed treatment: A predetermined amount of the above isothianyl aqueous suspension was coated on a germinated rice seed (variety: Nihonbare) with a shaking mixer, and then air-dried to prepare.

Test example Mitigating effect test of herbicidal compounds against paddy rice
(Method)
The seed-treated rice seeds were sown and grown in a pot (plastic, 100 cm ² ) filled with paddy soil in a greenhouse. A predetermined amount of the test herbicide diluted solution was sprayed from the upper part of the rice body at the 2.5 leaf stage of rice. One day after the treatment, the water depth was 3 cm, and 3 weeks after the treatment, the rice chemical damage was investigated. In the evaluation of paddy rice phytotoxicity, complete death was 100% and 0% was no phytotoxicity. The results are shown in Table 1.

Claims (4)

  A method of reducing phytotoxicity of a seed crop by treating seeds with 3,4-dichloro-isothiazole-5-carboxylic acid-2-cyanoanilide before sowing.   The method according to claim 1, wherein the seed crop is paddy rice, and the phytotoxicity of the paddy rice seedling after direct sowing of Honda is reduced.   The method according to claim 1, wherein the seed crop is paddy rice and the phytotoxicity during seedling raising in the seedling box is reduced.   The method according to claim 1, wherein the seed crop is paddy rice, and the phytotoxicity of the paddy rice seedling after transplanting to Honda is reduced.