TW201006383A

TW201006383A - Method of reducing phytotoxicity

Info

Publication number: TW201006383A
Application number: TW098111600A
Authority: TW
Inventors: Shinichi Shirakura
Original assignee: Bayer Cropscience Ag
Priority date: 2008-04-09
Filing date: 2009-04-08
Publication date: 2010-02-16
Also published as: JP2009249358A; BRPI0911629A2; KR20110002850A; WO2009124708A3; JP2011517679A; WO2009124708A2; CN101998826A

Abstract

A method of reducing phytotoxicity to seed crops by treating the seeds with 3,4-dichloro-2'-cyano-1,2-thiazole-5-carboxanilide before seeding.

Description

201006383 六、發明說明：【發明所屬之技術領域】本發明係有關一種降低植物毒性之方法。明確_之發明係有關一種以特定之化合物處理作物種 13之本作物之植物毒性之方法。降低其對【先前技術】公眾已知某些型態之異嗟唾羧醯胺（其 JP2刪522840T)具有降低植物毒性之活性$參見 JP2004346030A)。此外，亦已知在育苗箱中預先噴灑3‘二氯-2'-氰基-1，2-噻唑-5-曱醯替苯胺（俗名：柢具p " ’ . , A 1 ^^(lsotianil)) 時，可在秧苗移植至水田後有效降低除草劑對水性(參見JP20G7137833A)。植㈣近年來逐漸需要節省人力與低成本之__。因Μ 需要-種可直接栽種稻秧苗之作法，因為其可大幅降低 φ 時之勞力’如：在育苗箱中準備供移植種稻所需之秩苗。缺而，由於除草劑之植物毒性-般對直接_之稻對移植後栽種之稻，因此極需要儘快解決此問題。、【發明内容】 “本發明者已發現使用抑丹尼(is〇tianil)處理種子時，可顯 :降如··除草劑處理所引起之植物毒性，並發明。本發明亦可麟雜之細作物。糾理作物種子後再栽種，亦可在育苗期間及移植種子 201006383 後’付到其降低化學處理(例如：除草劑）對植物之毒性效應。【實施方式】可利用本發明方法降低植物毒性之除草活性化合物沒有特別限制，但其實例包括下列化合物：乙酿基CoA幾化酶(ACCase)抑制劑，芳基氧苯氧基-丙酸酯型ACCase抑制劑：炔草酸典 (clodinafop-propargyl)、丁基賽伏草（Cyhalofop-butyl)、禾草畏（diclofop-methyl)、芬殺草（feil〇xaprop-P-ethyl)、伏寄普 (fluazifop-P-butyl)、甲基合氯氟(hal〇xyf〇p-R-methyl)、普拔草（propaquizafop)、精禾靈（qUizalofop_P_ethyl)及崎唑草胺 (metamifop)；環己一酮型ACCase抑制劑：亞汰草（aii〇Xydim)、丁苯草 _(butroxydim)、勉草同（cieth〇dim)、環殺草(cycloxydim)、環本草嗣（profoxydim)、西殺草（seth〇xydim)、得殺草 (tepraloxydin)及肟草酮(tralkoxydim);及〇本基吼》1坐咐型ACCase抑制劑：《坐琳草醋(pinoxaden)。乙醢乳酸醋合成酶(ALS)抑制劑，續醯脲型ALS抑制劑：鳴確隆(amjd〇suifuron)、四嗤嘯石頁隆(azimsulfuron)、免速隆(bensuifuron_methyl)、氣β密績隆 (chlorimuron-ethyl)、氯黃隆（chl〇rsulfur〇n)、西速隆 (cinosulfuron)、環續隆（Cyci〇suifainuron)、胺苯續隆 201006383 (ethametsulfuron-methyl)、亞速隆（ethoxysulfuron)、伏速隆 (flazasulfuron)、氟咬癌石黃隆（flupyrsulfuron-methyl-Na)、甲胺石黃隆（负^1113111£\11：011)、氣吡痛續隆(1^1〇3111£>1111〇11-11^1^1)、依速隆(imazosulfuron)、峨曱續隆(iodosulfuron)、甲石黃胺磺隆 (1116303111包1*011-11^11丫1)、甲確隆(11^5111^11：011-11^1^1)、终嘧石黃隆（nicosulfuron)、環氧嘧確隆（oxasulfuron)、氟嘧橫隆 (primisulfuron-methyl)、百速隆（pyrazosulfuron-ethyl)、玉嘧績隆(rimsulfuron)、曱嘯石黃隆(sulfometuron-methyl)、續胺續隆（sulfosulfuron)、嗟吩績隆(thifensulfuron-methyl)、醚苯確隆（triasulfuron)、笨績隆（tribenuron胃methyl)、三氟咬續隆 (trifloxysulfuron)、氟胺續隆(triflusulfuron-methyl)、三氟曱續隆（tritosulfuron)、〇密苯胺確隆（orthosulfamuron)、TH547 及 NC620 ; °米唾琳_型(ALS)抑制劑：甲σ米唾於酸(imazapic)、11米草酸(imazamethabenz-methyl)、曱氧咪草煙（imazamox)、依滅草（imazapyr)、滅草（imazaquin)及咪唑乙於酸(imazethapyr); 三唑并嘧啶型 ALS抑制劑：氯酯磺草胺 (cloransulam-methyl)、雙氯石黃草胺(diclosulam)、雙氟續草胺 (florasulam)、°坐癌續草胺（flumetsulam)、確草唾胺（metosulam) 及平速爛(penoxsulam); 嘧啶基水揚酸酯型 ALS抑制劑：雙草醚 (bispyribac-Na)、响咬草醚（pyribenzoxim)、環酯草醚 (pyriftalid)、《密草硫鍵（pyrithiobac-Na)、嘴草醚 (pyriminobac-methyl)及必速吩(pyrimisulfan);及 201006383 二唾琳酮型ALS抑制劑：氟酮石黃隆(flucarbazone-Na)、丙本續隆（propoxycarbazone-Na)及賽續隆(thiencarbazone)。光合作用（光系統II)抑制劑，二σ井型：莠滅淨(ametryne)、草脫淨(atrazine)、氰乃淨 (cyanazine)、地草淨（desmetryne)、地戊乙淨 (dimethametryne)、撲滅通(prometon)、撲草淨(prometryne)、普拔根(propazine)、草滅淨(simazine)、西草淨(simetryne)、甲氧去草淨(terbumeton)、草淨津（terbuthylazine)、特丁淨 (terbutryne)及草達津(trietazine); 三0井_型：環噃酮(hexazinone)、苯嗓草嗣(metamitron) 及唤草酮(metribuzin); 三0坐琳酮型：氨嗤草酮（amicarbazone); 尿°密咬型：除草定(bromacil)、環草定(lenacil)及特草定 (terbacil)；塔0井酮型：氯草敏(chloridazon); 苯基-胺曱酸醋型：雙苯胺靈（desmedipham)及苯敵草 (phenmedipham)；尿素型：滅落寧（chlorobromuron)、綠麥隆 (chlorotoluron)、枯草龍（chloroxuron)、噚。坐隆（dimefuron)、達有隆(diuron)、續嗟隆(ethidimuron)、非草隆(fenuron)、伏草隆（fluometuron)、異丙隆(isoproturon)、愛速隆(isouron)、理有隆（linuron)、甲基苯隆（methabenzthiazuron)、撲多草 (metobromuron)、甲氧隆（metoxuron)、綠谷隆（monolinuron)、 201006383 草不龍(neburon)、環草隆(siduron)及丁噻隆(tebmhiuron); 醯胺型：除草靈(propanil)及曱氯草胺(pentanochlor); 腈型：溴殺烯(bromofenoxim)、溴苯腈(bromoxynil)及破苯(ioxynil); 苯并11塞二σ井酮型：本達隆(bentazon);及苯基-塔α井型：必汰草(pyridate)及嗟蟲琳(pyridafol)。經由光活化作用（光系統II)之毒性誘導劑，聯π比咬鑌型：敵草快(diquat)及巴拉別（paraquat)。原卟啉原氧化酶(PPO)抑制劑，二苯基醚型：亞喜芬(acifluorfen)、比芬諾(bifenox)、曱氧基護谷(chlomethoxyfen)、乙叛氟草_印11〇1'〇笆1>^(^11)、氟磺胺草醚（fomesafen)、禾賽吩（halosafen)、乳氟禾草靈 (lactofen)、復祿芬（oxyfluorfen)及曱氧基護谷 (chlomethoxynil)；苯基π比嗤塑：異丙草醋（fluazolate)及α比草鍵 (pyraflufen-ethyl)； N-苯基敌酿亞胺型：曱基辛登(cinidon-ethyl)、弗莫辛 (flumioxazin)及戊基弗莫辛(flumiclorac-pentyl); 嗟二哇型：亂乙草醋（fluthiacet-methyl)及°塞二0坐草胺 (thidiazimin)； 0号二唾型：樂滅草(oxadiazon)及炔吟草酿I(oxadiargyl); 三唾琳嗣型：草芬定（azafenidin)、乙基克繁草 201006383 (carfentrazone-ethyl)及曱磺草胺（sulfentrazone); 17号嗤咬二嗣型：B号嗓酮(pent〇XaZ〇ne); 鳴σ定二酮型：苯吩松（benzfendizone)及氟丙鳴草酯 (butafenacil);及其他.雙°坐草(pyraclonil)、氟°坐草胺(profluazol)及氟噠草酯（flufenpyr-ethyl)。類胡蘿蔔素生合成抑制劑， 1 · PDS抑制劑塔口井酮型：氟草敏(norflurazon); π比唆叛醯胺型：氟草胺（diflufenican)及β比氟苯草胺. (picolinafen);及其他：氟丁草胺(beflubutamid)、氟 β定草酮（fluridone)、氟口各草酮（flurochloridone)及草酮（flurtamone); 2. 4-HPPD抑制劑三酮型：硝草酮(mes〇tri〇ne)、續草酮（sulcotrione)、苯❹ 並雙環酮(benzobicyclon)及特草酮(tefuryltrione); 異0号°坐型：異4氣草嗣（isoxachlortole)及異°号°坐草_ (isoxaflutole)； σ比0坐型：〇比草酮(benzofenap)、°比0坐特(pyrazolynate)及节草唾(pyrazoxyfen);及其他：苯並雙環酮(benzobicyclon);及 201006383 3.未知標的二0坐型·氨基三唾(amitrole); 異口寻唾°疋®同型：可滅蹤(clomazone);及一本基鍵型：苯草謎(aci〇nifen)。 EPSP合成酶抑制劑，甘胺酸型：草甘膦（glyphosate)及草甘膦三甲基硫鹽 (glyphosate-trimesium)。麵酿胺合成酶抑制劑，膦酸型·固殺草(gluf〇sinate)及雙丙胺膦(biaiaph〇s)。 DHP生合成抑制劑，胺甲酸酯型：亞速爛（asulam)。微小管組合之抑制劑，二硝基苯胺型：氟草胺(bethrodine)、比達寧(butralin)、撻乃安（dinitramine)、烯氟靈（ethalfluralin)、氨磺樂靈 (oryzalin)、施得圃（pendimethalin)及三福林(trifluraiin); fee基填酸醋型：曱基胺草填(amiprophos-methyl)及抑草填(butamiphos); °比咬型·汰硫草(dithiopyr)&n塞草定(thiazopyr);及本甲酿胺型：块苯草胺(pr〇pyzamide)、牧草胺(tebutam) 及氧酸二曱醋（chlorthal-dimethyl); 201006383 有絲分裂/微小管組織化之抑制劑，胺甲酸醋型：克普芬(chlorpropham)、苯胺靈(propham) 及雙草胺（carbetamide)。極長鍵脂肪酸生合成之抑制劑，氣乙酿胺型：乙草胺(Acetochlor)、拉草（Alachlor)、丁草胺（butachlor)、二甲草胺（dimethachlor)、汰草滅 (dimethanamid)、滅草胺(metazachlor)、莫多草(metolachlor)、烯草胺（pethoxamid)、普拉草（pretilachlor)、雷蒙得 (propachlor)、異丙草胺（propisochl〇r)及甲氧草胺 (thenylchlor)；乙醢胺型：大芬滅（diphenamid)、滅落脫(napropamide) 及萘普草（naproanilide); 氧乙醯胺型：氟草胺(flufenacet)及滅芬草(mefenacet); 四哇琳酮型：四。坐草胺（fentrazamide);及其他：莎稗填（anilofos)、苯酮唾（cafenstrole)及草填 (piperophos)。纖維素生合成之抑制劑，腈型：敵草（dichlobenil)及氣硫胺（chlorthiamid); 苯甲酿胺型：異。号草胺(iS0Xaben); 三0坐并緩醯胺型：氣胺草嗤(fiUp0xam);及喧琳竣酸型：快克草（quinclorac)。 201006383 去偶合劑，二硝基酴型：DNOC、達諾殺（dinoseb)及特樂酴 (dinoterb) ° 脂肪酸延長抑制劑（未抑制ACCase)，硫代胺曱酸酯型：拉敵草(butylate)、草滅特(CyCi〇ate)、呱草丹（dimepiperate)、EPTC、戊草丹（esprocarb)、草達滅 (molinate)、坪草丹（orbencarb)、克草猛（pebulate)、草丹 (prosulfocarb)、殺萘丹(benthiocarb)、稗草丹(pyributycarb)、仲草丹(tiocarbazil)、野麥畏(triaiiate)及萬隆(vernolate；) 二硫代磷酸S旨型：地散填(bensulide) 苯并17夫喃型.草黃(benfuresate)及乙氧草黃(ethofumesate) 及氯-碳酸型：TCA、得拉本（dalapon)及四氟丙酸 (tetrapion)。似植物生長素除草劑，苯氧基-羧酸型：克普草（clomeprop)、2,4-D、2,4-DB、滴丙酸(dichlorprop)、MCPA、MCPB 及 MCPP ; 苯甲酸型：草滅畏（chloramben)、汰克草（dicamba)及 2,3,6-TBA ； π比唆叛酸型：畢克草(clopyralid)、氟氣比(fluroxypyr)、氨氯咬酸(picloram)、三氣比(triclopyr)、快克草（quinclorac) 及氯曱酸(quinmerac)及 -11 - 201006383 其他：草除靈(benazolin-ethyl)。植物生長素轉運抑制劑，鄰苯酿胺型：納得爛(naptalam);及縮苯胺基脲型：二氟π比隆(diflufenzopyr-Na)。其他(作用模式未知者），201006383 VI. DESCRIPTION OF THE INVENTION: TECHNICAL FIELD OF THE INVENTION The present invention relates to a method for reducing phytotoxicity. It is clear that the invention relates to a method for treating the phytotoxicity of a crop of a crop species 13 with a specific compound. Reducing the Pairs [Prior Art] Certain types of isoindoles (which are JP2 522840T) are known to the public to have phytotoxicity reducing activity (see JP2004346030A). In addition, it is also known to pre-spray 3'-dichloro-2'-cyano-1,2-thiazol-5-indolediamine in the nursery box (common name: cookware p " ' . , A 1 ^^( When lsotianil)), the herbicide is effectively reduced in water after transplanting the seedlings to the paddy field (see JP20G7137833A). Planting (4) In recent years, there has been a gradual need to save manpower and low cost. Because of the need, you can directly plant rice seedlings because it can greatly reduce the labor of φ. For example, prepare the seedlings needed for transplanting rice in the nursery box. In the absence of phytotoxicity of herbicides, it is highly desirable to solve this problem as soon as possible. [Summary of the Invention] "The present inventors have found that when the seed is treated with is〇tianil, the phytotoxicity caused by the herbicide treatment can be reduced and invented. The present invention can also be used. Fine crops. After cultivating crop seeds, planting them can also be used to reduce the toxic effects of chemical treatments (eg herbicides) on plants during seedling and after transplanting seeds 201006383. [Embodiment] The method of the invention can be used to reduce The phytotoxic herbicidal active compound is not particularly limited, but examples thereof include the following compounds: an amine-based CoA numbering enzyme (ACCase) inhibitor, an aryloxyphenoxy-propionate type ACCase inhibitor: clodinafop -propargyl), Cyhalofop-butyl, diclofop-methyl, feilixaprop-P-ethyl, fluazifop-P-butyl, methyl Chlorofluoride (hal〇xyf〇pR-methyl), propaquizafop, qUizalofop_P_ethyl, and miamifop; cyclohexanone-type ACCase inhibitor: tibia (aii〇) Xydim), butyl benzoate _ (butroxydim), Cieth〇dim, cycloxydim, profoxydim, seth〇xydim, tepraloxydin and tralkoxydim; and 〇基基吼》1 sitting ACCase inhibitor: "pinoxaden". Acetyl lactic acid vinegar synthase (ALS) inhibitor, continued ureac type ALS inhibitor: Amijd〇suifuron, Si Xiaoxiao Azimsulfuron, bensuifuron_methyl, chlorimuron-ethyl, chl〇rsulfur〇n, cinosulfuron, Cyci〇suifainuron ), anthraquinone 201006383 (ethametsulfuron-methyl), ethoxysulfuron, flazasulfuron, flupyrsulfuron-methyl-Na, methotrexate (negative ^1113111 £\11:011), Qipi painful swell (1^1〇3111£>1111〇11-11^1^1), iazosulfuron, iodosulfuron, sarcophagus Sulfonamide (1116303111 pack 1*011-11^11丫1), Jiazhenglong (11^5111^11:011-11^1^1), nicosulfuron, epoxy pyrimidine (oxasulfuron), Primisulfuron-methyl, pyrazoosulfuron-ethyl, rimsulfuron, sulfometuron-methyl, sulfosulfuron, 嗟绩隆隆(thifensulfuron-methyl), triasulfuron, tribenuron gastric methyl, trifloxysulfuron, triflusulfuron-methyl, tritosulfuron , orthosulfamuron, TH547 and NC620; °Salina _ type (ALS) inhibitors: imazapic, imazamethabenz-methyl, oxime (imazamox), imazapyr, imazaquin, and imidazepyr; triazolopyrimidine ALS inhibitor: cloransulam-methyl, diclofenac Amine (diclosulam), diflufenthine (florasulam), flumetsulam, metosulam, and penoxsulam; pyrimidine-based salicylate-type ALS inhibitors: Bispyribac-Na, pyribenzoxim, pyrifta Lid), "pyrithiobac-Na", pyriminobac-methyl and pyrimisulfan; and 201006383 stilbenone-type ALS inhibitor: flucarbazone-Na ), propoxycarbazone-Na and thiencarbazone. Photosynthesis (photosystem II) inhibitors, two σ well types: ametryne, atrazine, cyanazine, desmetryne, dimethametryne , prometon, prometryne, propazine, simazine, simetryne, terbumeton, terbuthylazine , terbutryne and trietazine; tri-zero well _ type: hexazinone, metamitron and metribuzin; Amicarbazone; urine type: bromacil, lenacil, and terbacil; ketone type: chloridazon; phenyl- Amine vinegar type: desmedipham and phenmedipham; urea type: chlorobromuron, chlorotoluron, chloroxuron, strontium. Dimefuron, diuron, ethidimuron, fenuron, fluometuron, isoproturon, isouron, Linuron, methabenzthiazuron, metobromuron, metoxuron, monolinuron, 201006383 neburon, siduron, and thiothiazol (tebmhiuron); guanamine type: propanil and pentanochlor; nitrile type: bromofenoxim, bromoxynil and ioxynil; benzo 11 Sigma ketone type: Bentazon; and phenyl-tower alpha well type: pyridate and pyridafol. Through the photoinitiation (photosystem II) toxicity inducer, the π ratio bite type: diquat and paraquat. Protoporphyrinogen oxidase (PPO) inhibitor, diphenyl ether type: acifluorfen, bifenox, chlomethoxyfen, acetaminophen _印11〇1 '〇笆1>^(^11), fomesafen, halosafen, lactofen, oxyfluorfen, and chlomethoxynil Phenyl pi to bismuth: fluazolate and alpha-grass (pyraflufen-ethyl); N-phenyl-entitled-type: cinidon-ethyl, fluoxine Flumioxazin) and flumiclorac-pentyl; 嗟哇 wow type: fluthiacet-methyl and thidiazimin; No. 2 di-salt: Lemgrass ( Oxadidiazon and acetyldiargyl; three saliva-type: azafenidin, ethyl gram grass 201006383 (carfentrazone-ethyl) and sulfentrazone;嗣 type: B B a 〇〇〇〇〇〇〇〇〇〇〇〇定定定定定定定定定定定 ben ben 定定定定定定定 ben 定定定 ben ben ben ben ben ben ben ben ben ben ben ben ben ben ben ben ben ben ben ben ben ben ben °Salvain (proflua Zol) and flufenpyr-ethyl. Carotenoid biosynthesis inhibitor, 1 · PDS inhibitor Tarantan type: Norflurazon; π than 唆唆醯 : type: diflufenican and β fenfluramine. (picolinafen And others: beflubutamid, fluridone, flurochloridone and flurtamone; 2. 4-HPPD inhibitor triketone: nitriceps Ketone (mes〇tri〇ne), sulcotrione, benzobicyclon and tefuryltrione; different 0°° sitting: isoxachlortole and iso No. ° isoxaflutole; σ ratio 0 sitting type: benzofenap, pyrazolynate and pyrazoxyfen; and others: benzobicyclon; And 201006383 3. Unknown target two zero-sitting amino-satellite (amitrole); different mouths to find saliva® 同 type: can be eliminated (clomazone); and a base bond type: aci〇nifen (aci〇nifen). EPSP synthetase inhibitor, glycine type: glyphosate and glyphosate-trimesium. Surface amine-synthesizing enzyme inhibitor, phosphonic acid type gluf〇sinate and biiaphine sulphate. DHP biosynthesis inhibitor, carbamate type: sub-aluminum (asulam). Inhibitors of microtubule combination, dinitroaniline type: bethrodine, butralin, dinitramine, ethalfluralin, oryzalin, sedative (pendimethalin) and trifluraiin; fee base-filled vinegar type: amiprophos-methyl and (butamiphos); ° ratio bite type thiophena (dithiopyr) &Thiazodazole; and the present amylamine type: pr〇pyzamide, tebutam and chlorthal-dimethyl; 201006383 inhibition of mitosis/microtubule organization Agent, urethane type: chlorpropham, propham and carbeamide. Inhibitor of very long-chain fatty acid biosynthesis, acetochlor, Alachlor, butachlor, dimethachlor, dimethanamid , metazachlor, metolachlor, pethoxamid, pretilachlor, propachlor, propisochl〇r, and methoxychlor (thenylchlor); acetaminophen type: diphenamid, napropamide and naproanilide; oxyethylamine type: flufenacet and mefenacet; Four wow ketone type: four. Fentrazamide; and others: anilofos, cafenstrole, and piperophos. Inhibitors of cellulose biosynthesis, nitrile type: dichlobenil and chlorthiamid; benzoic acid type: different. Isooxamin (iS0Xaben); three zero sitting and slowing amine type: acetaminophen (fiUp0xam); and 喧竣 acid type: quinclorac (quinclorac). 201006383 Decoupling agent, dinitroguanidine type: DNOC, dinoseb and dinoterb ° fatty acid elongation inhibitor (not inhibited ACCase), thioamin phthalate type: butylate ), CyCi〇ate, dimepiperate, EPTC, esprocarb, molinate, orbencarb, pebulate, grass dan (prosulfocarb), benthiocarb, pyributycarb, tiocarbazil, triaiiate and vernolate; dithiophosphoric acid S: ground filling ( Bensulide) Benzosate type, benfuresate and ethofumesate and chloro-carbonate type: TCA, dalapon and tetrapion. An auxin herbicide, phenoxy-carboxylic acid type: clomeprop, 2,4-D, 2,4-DB, dichlorprop, MCPA, MCPB and MCPP; benzoic acid type : chloramben, dicamba and 2,3,6-TBA; π than 唆唆 acid type: clopyralid, fluorine ratio (fluroxypyr), ammonia chloride (picloram) ), triclopyr, quinclorac and quinmerac and -11 - 201006383 others: benazolin-ethyl. An auxin transport inhibitor, o-phenylamine type: naptalam; and anilino urea type: diflufenzopyr-Na. Other (the mode of action is unknown),

芳基胺基丙酸型：麥草氟曱醋(flamprop-M-methyl)及麥草氟異丙醋（flamprop-isopropyl); π比唾嘛翁型：野麥枯(difenzoquat); 有機砷型：DSMA及MSMA ;及其他·漠芬諾（bromobutide)、氯伏諾（chloroflurenol)、 J衣庚草 _(cinmethylin)、苄草隆(cumyluron)、邁隆(dazomet)、殺草隆(daimuron)、曱基達草隆(methyl-dimuron)、乙氧苯草胺（etobenzanid)、殺木膦（fosamine)、節草酮（indan〇fan)、滅丹(metam)、°惡嗪草_(〇xaziclomefone)、油酸、壬酸及稗草丹(pyributicarb)。 ❹ 此等除草化合物（以俗名或衍生物名稱說明）主要說明於例如：”農藥手冊(The Pesticide Manual)”，第Η版(英國作物保濩協會(British Crop Protection Council)，2007)，或係已知者。 ’、根據本發明方法，可在作物之雜草中達到選擇性除草效果。本文中，雜草之廣義定義係指生長在不期望其生長之地點上之任何植物。例如：下文說明之雜草與作物可作為應用 •12- 201006383 本發明方法之主題。雙—子秦植短趣農丄白齐(sinapisj、薺菜、獨行条（Leipidium)、豬殃殃（Ga丨ium)、鵝陽茱（SteUaria)、緣 (Chenopodium)、也瓊（K〇chia)、蓴蘇（加心）、瓜葉菊 (¾1舰㈣」、莧以所卿㈣叫）、馬齒莧「户、苔耳 (Xanthium)、奪 + 疋（Ip〇m〇ea)、毛蓼、ρ〇1Μ〇η·)、豚專、玉山薊（c/r幻·謂）、鬼苦苣菜^⑽以叫^、茄 ❹ (Soianum)、虱花菜（R〇rippa)、野芝酞（Lamium)、水苦寬 (Teromca;、洋金花少扣_)、三色堇鼬瓣花 (Galeopsis)、罂粟（Papa曹）、矢萆菊（Cen_rea)、+ 米菊 «如gq；、節茚草⑺加、陌上菜、田菁 (Sesbania)、三案萆（Trifolium)、蔥氟（AbutU〇n)、野乏龜 (Lamium)、母萆（Matricaria)、艾萆（Artemisia)、田濟 (Sesbania)、牽年(Pharbitis)，蓴專雙子葉植处:棉花（Go*w;;pz··)、大豆（G(ycz>^、甜菜 ❹ （"如叫、紅蘿蔔、四季豆fPhaseolusJ、豌豆麵）、染（Solanum)、备氣(Linum)、養蹇(Ipomoea)、ff 窥 l(Vicia)、煙專(Nicotiana)、％ % (Lycopersicon) m(Arachis)、蕓笔 (Brassica)、氣 1 (Lactuca)、小責瓜（Cucumis)、南版 (Cucurbita)，專專。早....子_葉.雜卓_ ••稗（Echinochha)、赛草（Setaria)、薇、馬唐(/)初加η·α)、梯牧草(Phleum)、長齒草(户⑽）、牧場草（Fe对wcfl)、蟋蟀草（五/從幻k)、毒麥(ζ〇々ΜΑ„)、雀麥 (5roww·?)、燕麥(Jvewa)、莎草、高粱（5Wg"/zwm)、小 -13- 201006383 麥草、雨久花、飄拂草 (Fimbristyslis)、亀姑（Sagittaria)、烏 ^(EleochaHs)、瑤萆价吵奶）、雀禆（Pi^tf/ww)、鴨嘴草（ZycAae/w麵）、糠穗沿）、看麥娘(Aopecwms)、百慕達草（Q^oi/洲）、鴨路萆（Commelina)、巴拉萆（Brachiaria)、織子萆(Leptochloa)，等等。 U葉植物:稻（Οπθ、玉米（Zey、小麥(7W"cwmJ、大麥卜燕麥⑼以、黑麥阶、高粱降)rg/2_J、大黍㊇叫、甘蔗P娜/^謂J、芭蕉（Μ謹；、鳳梨® 、蘆筍、蔥，等等。此外’本發明該方法並不限於上述植物，其亦可依類似方式用於其他相關植物。此外，根據本發明該方法可用於在多年生作物中控制雜草，且可施用於例如：森林、觀賞性造林、果園、葡萄園、柑橘林、核果園、香蕉園、咖啡園、茶園、橡膠園、油棕櫚園、可可園、軟果園、及啤酒花園，等等。其亦可用在一年生作物中選擇性控制雜草。 ❹ 本發明方法可在水稻與水稻雜草之間提供選擇性控制除草效果。因此，可以控制之水稻田雜草實例如下：王列早子葉_撞_^ :毛蓼(Τ〇丨ygonum)、風花茱(R〇rippa)、節節草⑺⑷、陌上菜仏〜办⑺叫、鬼針草⑺眼萆（D〇patrium)、墨茱(脑pta)、溝繁縷(£!atine)、水八魚 (Gmtwla)、陌上菜(Lindern⑷、水丁香认以心^⑷、水芹茱 (0細、毛貧(杨隊、澤番椒⑺咖⑽刪」，等等。 201006383 下列么子__盡植物.稗(Echinochloa)、稷、長齒草（尸⑽）、莎草（QwerMs)、雨久花（儿/〇⑽c/^咖）、飄拂草 (FimbHstyslis)、亀姑（Sagittaria)、·％芊[Eleocharis)、Μ 草 (SWr/ms)、澤瀉〇4//洲fl>)、水竹葉⑽y、簀藻⑺如^、鼓精萆（EHocaubn)、眼子茱（j>otamogeton)、巴拉草 (Bmchiaria)、纖 + 萆(Leptochloa)、災瓣花XSphenoclea),專等。更明確言之，本發明方法可應用於例如：下列典型水稻雜草。植物名稱葉：拉丁學名田字草 Marisilea quadrifolia 水虱草 Fimbristylis miliacea 尖瓣花 Sphenoclea zeylanica 千屈菜 Ammannia sp. 印度茚茚草 Rotala indica Koehne 陌上菜 Lindernia procumbens Philcox 山莧菜 Amaranthus viridis 美洲母草 Lindernia dubia L. Penn. 纏腸 Eclipta prostrata 長果母草 Lindernia angustifolia 毛葉腎蕨 Ludwigia prostrata Roxburgh 毛蓼 Polygonum sp. -15- 201006383 田菁眼子菜多窪牽牛長莖溝繁縷爪哇水芹菜單子葉：禾稗芒稷野稗鴨嘴草稷草牛筋草馬唐草雙穗雀稗碎米莎草香附子牛毛氈荸薺異花莎草水莎草水毛花田蒜仔大井氏水莞Arylaminopropionic acid type: flamprop-M-methyl and flamprop-isopropyl; π than saliva type: difenzoquat; organic arsenic: DSMA And MSMA; and others · bromobutide, chloroflurenol, cinmethylin, cumyluron, dazome, daimuron, medlar Methyl-dimuron, etobenzanid, fosamine, indan〇fan, metam, oxazinium _(〇xaziclomefone) , oleic acid, citric acid and pyributicarb. ❹ These herbicidal compounds (described by common name or derivative name) are mainly described, for example, in “The Pesticide Manual”, the third edition (British Crop Protection Council, 2007), or Known. According to the method of the present invention, selective herbicidal effects can be achieved in weeds of crops. As used herein, the broad definition of weed refers to any plant that grows on a point where it is not expected to grow. For example, the weeds and crops described below can be used as the subject of the method of the invention. 12-201006383. Shuang-zi Qin Zhi short fun farmer white Qi (sinapisj, leek, Leipidium, Hogium, SteUaria, Chenopodium, K〇chia) , Susu (plus heart), cineraria (3⁄41 ship (four)", 苋所所卿 (4), purslane "Xanthium", Xenthium, Ip〇m〇ea, 〇 Μ〇 Μ〇 ) ) 豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚豚Lamium), water-rich (Teromca;, golden flower less buckle _), three-color flower (Galeopsis), poppy (Papa Cao), yarrow (Cen_rea), + Miju «such as gq; Valerian (7) Plus, Moshang, Sesbania, Trifolium, Abut U〇n, Lamium, Matricaria, Artemisia, Tian Sesbania, Pharbitis, 双双双双双 : cotton (Go*w;; pz··), soybean (G (ycz>^, beet ❹ ("called, carrot, four seasons Bean fPhaseolusJ, pea face), dyed (Solanum), gas (Linum), raising carp ( Ipomoea), ff Vicia, Nicotiana, %% (Lycopersicon) m (Arachis), Brassica, Lactuca, Cucumis, Cucurbita , special. Early....子_叶.杂卓_ ••稗(Echinochha), Setaria, Wei, Matang (/) first η·α), Timing grass (Phleum), long Tooth grass (household (10)), pasture grass (Fe vs. wcfl), alfalfa (five/from magical k), poisonous wheat (ζ〇々ΜΑ„), buckwheat (5roww·?), oatmeal (Jvewa), sedge , sorghum (5Wg"/zwm), small-13-201006383 wheat straw, rain long flower, Fimbristyslis, Sagittaria, EleochaHs, Yao 萆吵、, 禆禆 (Pi^ Tf/ww), platypus (ZycAae/w), 糠沿), Aopecwms, Bermuda (Q^oi/洲), Commelina, Brachiaria ), Leptochloa, and so on. U-leaf plants: rice (Οπθ, corn (Zey, wheat (7W" cwmJ, barley oats (9), rye, sorghum) rg/2_J, 黍黍, sugar cane P Na / ^ said J, plantain ( Μ ;;, pineapple®, asparagus, onion, etc. Further, the method of the present invention is not limited to the above plants, and can be applied to other related plants in a similar manner. Further, according to the present invention, the method can be applied to perennial crops. Control weeds and apply to, for example, forests, ornamental afforestation, orchards, vineyards, citrus forests, nuclear orchards, banana gardens, coffee gardens, tea gardens, rubber plantations, oil palm gardens, cocoa gardens, soft orchards, and Beer garden, etc. It can also be used to selectively control weeds in annual crops. ❹ The method of the invention can provide selective control of herbicidal effects between rice and rice weeds. Therefore, examples of controllable rice field weeds are as follows : 王列早子叶_撞_^ : 毛蓼(Τ〇丨ygonum), 风花茱(R〇rippa), 节节草(7)(4), 陌上菜仏~办(7)叫,鬼针草(7)眼萆(D〇 Patrium), ink 茱 (brain pta), 沟繁缕 (£! atine) Water octopus (Gmtwla), Moss (Lindern (4), water cloves recognize heart ^ (4), water cane (0 fine, Mao poor (Yang team, Zefan pepper (7) coffee (10) deleted", etc. 201006383 The following __All plants. Echinochloa, cockroach, larvae (10), sedge (QwerMs), rain long flower (child / 〇 (10) c / ^ coffee), mb草草 (FimbHstyslis), 亀姑 (Sagittaria) , ·% 芊[Eleocharis), Μ草(SWr/ms), Alisma 〇4//洲fl>), water bamboo leaves (10)y, algae (7) such as ^, Ehocaubn, eye 茱 (j&gt ; otamogeton), Bmchiaria, Leptochloa, XSphenoclea, special. More specifically, the method of the present invention can be applied to, for example, the following typical rice weeds. Latin name Marisilea quadrifolia sylvestris Fimbristylis miliacea sphenoclea zeylanica sylvestris Ammannia sp. Indian sedge Rotala indica Koehne sinensis Lindenhus procumbens Philcox scallions Amaranthus viridis sylvestris Linden dubia L. Penn. Intestine Eclipta prostrata Long Motherwort Lindernia Angustifolia hairy kidney fern Ludwigia prostrata Roxburgh buttercup Polygonum sp. -15- 201006383 Tian Jing eyed vegetable 洼洼长 long stem ditch 缕缕缕水水菜单菜单菜单菜单菜单菜单 : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : Double-spotted snails, snails, stalks, stalks, stalks, stalks, stalks, stalks, stalks

Sesbania exaltata Potamogeton distinctus A. Benn Ipomoea lacunosa Elatine triandra Schk Oenanthe javanicaSesbania exaltata Potamogeton distinctus A. Benn Ipomoea lacunosa Elatine triandra Schk Oenanthe javanica

Echinochloa oryzicola Vasing Echinochlor colonum E. crus-galli Ischaemum rugosum Panicum sp.Echinochloa oryzicola Vasing Echinochlor colonum E. crus-galli Ischaemum rugosum Panicum sp.

Eleusine indica Digitaria sp.Eleusine indica Digitaria sp.

Paspalum distichum Cyperus iria C. rotundusPaspalum distichum Cyperus iria C. rotundus

Eleocharis acicularis L. Eleochris kuroguwai Ohwi Cyperus difformis L.Eleocharis acicularis L. Eleochris kuroguwai Ohwi Cyperus difformis L.

Cyperus serotinus Rottboel Scirpus mucronatus S. planiculmis Scirpus juncoides Roxburgh -16- 201006383 鴨舌草瓜皮草窄葉澤瀉澤瀉二鲫剪(野慈菇) 雨久花全草 ❹ 巴扱草中國蟣子草Per tin tin Cy Cy Cy Cy Cy Cy Cy Cy Cy Cy Cy Cy Cy Cy Cy Cy Cy

Monochoria vaginalis Presl Sagittaria pygmaea Miq Alisma canaliculatum A. Br. Bouche A, plantago-aquatica Sagittaria trifolia Monochoria korsakowii Brachiaria plantaginea Leptochloa chinensis 本發明方法不限於上述雜草。其亦可應用於其他類雜草。 ^進行本發明方法時’在播種至育苗箱、水稻田或立陵地之刚，先以含抑丹尼之組合物處理催苗或未催苗狀態之作物種子。明確言之，可使用組合物採用浸泡法或塵粉包覆法處理作物種子。處理之結果可使育苗箱巾、移植至水田後、直接播種至水田後或種於丘陵地上之作物受到之植物毒性降低。根據本發明之處理法可用於處理基因改造生物體 (GMO)，例如：植物或種子。基因改造植物（或基因轉殖植物) 為植物巾異絲目已敎整合“基因組巾。術語“里源基因”基本上係指將所提供之基㈣在植株外組成之基因引入基因轉殖獅之細隸細組、_素細㈣粒線體基因組中，藉由表現所需蛋白質或多肽，或下調或靜默處理植物 -17- 201006383 中另一種或其他基因（例如：利用反義技術、共壓抑技術或 RNAi技術[RNA干擾術])，賦與新穎或改良之農藝或其他性質。存在於基因組中之異源基因亦稱為轉殖基因。將轉植基因界定在植物基因組中之專一位置稱為轉化作用或基因轉殖作用。依植物品系或植物栽培品種、其位置與其生長條件（土壤、氣候、生長期、營養）而定，根據本發明處理法亦可造成超加成性(“增效”)效力。因此，例如：可能出現下列超過實際所預期之效力：降低施用率與/或擴大活性範圍與/或提高® 根據本發明可使用活性化合物與組令物之活性、改善植物生長、提高對高溫或低溫之耐受性、提高對乾旱或水或土壤鹽份之耐受性、提高開花率、簡化收成、加速成熟、提高產量、增大果實、增加植物高度、加深葉子綠色、提早開花、改善所收成產品之品質與/或提高營養價值、提高果實糖度、改善所收成產品之儲存能力與/或可加工性。較適合根據本發明處理之植物與植物栽培品種包括具有會影響此等植物之特別有利且適用特性之遺傳材料之所❹ 有植物（不論經由育種與/或生物技術方式取得者）。亦適合根據本發明處理之植物與植物栽培品種為彼等對種或多種生物壓力產生抗性之植物，亦即該植物與微生物病害展現更佳防雜，如：對勿植物病原性錢、Μ、病毒與/«貞絲。端、亦適合根據本發明處理之植物與植物栽培品種為、-種或多種非生物壓力因子產生抗性之植物。非生物愿力 201006383 條件可包括例如：乾旱、曝露在低溫下、曝露在高溫下、滲透壓力、水患、土壤鹽份提高、礦物濃度提高、曝露到臭氧、曝露到強光、氮營養素利用度受限、磷營養素利用度受限、避免遮蔭。Monochoria vaginalis Presl Sagittaria pygmaea Miq Alisma canaliculatum A. Br. Bouche A, plantago-aquatica Sagittaria trifolia Monochoria korsakowii Brachiaria plantaginea Leptochloa chinensis The method of the present invention is not limited to the above weeds. It can also be applied to other types of weeds. ^When performing the method of the present invention, the seed of the seedling or the seedling state is first treated with the composition containing the dandy in the seedling box, the paddy field or the ridge. Specifically, the crop seeds can be treated by soaking or dust coating using the composition. As a result of the treatment, the phytotoxicity of the seedling box towel, the transplanted to the paddy field, the direct sowing to the paddy field or the crop planted on the hilly land can be reduced. The treatment according to the invention can be used to treat genetically modified organisms (GMOs), such as plants or seeds. Genetically modified plants (or genetically transformed plants) have been integrated into the "genome towel" for the plant towel. The term "library gene" basically refers to the introduction of the gene provided by the base (4) outside the plant into the genetically-transferred lion. In the fine-grained, _ fine (four) mitochondrial genome, by expressing the desired protein or polypeptide, or down-regulating or silently treating another gene or other gene in plant-17-201006383 (eg, using antisense technology, Repressive technique or RNAi technique [RNA interference], conferring a new or improved agronomic or other property. The heterologous gene present in the genome is also known as the transgenic gene. The transgenic gene is defined in a specific location in the plant genome. It is called transformation or gene transfer. Depending on the plant line or plant cultivar, its location and its growth conditions (soil, climate, growth period, nutrition), the treatment according to the invention can also cause superadditiveness (" "Enhanced" efficacy. Thus, for example, the following may be expected to exceed the actual expected effect: reducing the rate of application and/or expanding the range of activity and/or increasing ® according to the invention Activity of active compounds and components, improving plant growth, increasing tolerance to high or low temperatures, increasing tolerance to drought or water or soil salinity, increasing flowering rate, simplifying harvest, accelerating maturity, increasing yield, Increasing fruit, increasing plant height, deepening leaf green, early flowering, improving the quality of the harvested product and/or improving nutritional value, increasing fruit sugar content, improving storage capacity and/or processability of the harvested product. The plant and plant cultivars treated by the invention include those plants (whether obtained by breeding and/or biotechnological means) having genetic material that would affect the particularly advantageous and suitable properties of such plants. Also suitable for treatment according to the invention Plants and plant cultivars are plants that are resistant to one or more kinds of biological stress, that is, the plants and microbial diseases show better anti-hybrid, such as: Phytopathogenic money, cockroaches, viruses and /{贞丝Plants and plant cultivars suitable for treatment according to the invention are, for example, one or more abiotic stress factors are produced Resistant plants. Non-biological will 201006383 conditions may include, for example, drought, exposure to low temperatures, exposure to high temperatures, osmotic pressure, flooding, increased soil salinity, increased mineral concentrations, exposure to ozone, exposure to glare, Nitrogen nutrient availability is limited, phosphorus nutrient availability is limited, and shading is avoided.

^ 亦可根據本發明處理之植物與植物栽培品種為彼等特徵在於加強收成特性之植物。提高該植物收成之方法可為例如.改善植物生理、生長與發展，如：水利用效率、水保留 =率:改善氮利用率、加強碳同化作用、改善光合作用、提鬲發芽效率與加速成熟。可進一步藉由改善植物結構(承受壓 ^下與無壓力條件下)來改善收成，包括(但不限於）：提早開花'控制開花以產生雜交種子、幼苗生長力、植物大小、節 =量與距離、根部生長、種子大小、果實大小、果笑大小、鐘it數量、、錢或每穗之種子數量、種子生質量、加強说必a度' 咸》種子散落、減少果莢開裂與倒伏抗性。其 =收成特性包括種子组成，如：碳水化合物含量、蛋白質含含讀組成、營養價值、降低不良營養素化合物、改善可加工性及改善儲存安定性。長力本f明可處理之植物為已表現雜種優勢或雜交生 ^物_^植物，—般可提高產量、生長力、健康及對取自細力因子之抗性。此等植物之典型製法為自父系之料雜親本(母本)與另 (父本)雜交。雜交種子典型地传 H父系雄雛親本隹出紙认也係自雄不稔性植物收成，然後 201006383 法為以植物基因組中之基因決定子造成雄不稔。此時且尤其當需要自雜交植物收成種子作為所需產物時，通常適用之作法為確保雜交植物完全恢復雄稔性。其作法為確保父本具有適當稔性恢復基因，可使包含負責雄不稔性之基因決定子之雜交植物恢復雄稔性。雄不稔性之基因決定子可位在細胞質中。細胞質雄不稔性(CMS)實例說明於例如：十字花科品種 (蕓苔（Brassica)屬種）。然而，雄不稔性之基因決定子亦可位在細胞核基因組中。雄不稔性植物亦可採用植物生物技術方法取得，如：遺傳工程法。取得雄不稔性植物之特別適用方❹ 法說明於WO 89/10396，其中例如··使核糖核酸酶，如： b arn a s e選擇性表現在雄蕊之胞子囊膜細胞中。藉由核糖核酸酶抑制劑，如：barstar在胞子囊膜細胞中之表現即可恢復稔性。〜可根據本發明處理之植物或植物栽培品種（可由植物生物技術方法，如：遺傳工程法得到）可為除草劑耐受性植物，亦即可以耐文一種或多種特定除草劑之植物。此等植物可採用基因轉化法或選拔包含賦與此等除草劑耐受性之突❹ 植物取得。除草劑耐受性植物為例如：草甘膦(glyph〇sateW受性植物’亦即可以耐受草甘膦除草劑或其鹽之植物。植物可以透過許多不同方絲耐受草甘膦。例如：草賴耐受性植物可耩由編碼酵素5-婦醇丙醜基莽草酸_3_磷酸醋合成酶 (EPS叫之基因轉化植物後形成。此等Epsps基因實例為沙門氏菌⑼以坤如狀以叫細菌之Αγ〇α基因（突變株 -20- 201006383 CT7)、農桿菌屬㈣卬入細菌之cp4基因、編碼倭牵牛 EPSPS、番茄 EPSPS 或牛筋草 EPSPS (WO 2001/66704) 之基因。其亦可為突變之EPSPS〇草甘膦_耐受性植物亦可藉由編碼草甘膦氧化還原酶酵素之基因表現製得。草甘膦耐艾性植物亦可藉由編碼草甘膦乙醯基轉移酶酵素之基因表現製得。草甘膦耐受性植物亦可藉由選拔包含上述基因之天然突變之植物製得。Plants and plant cultivars which may also be treated according to the invention are plants which are characterized by enhanced harvest characteristics. Methods for improving the plant harvest can be, for example, improving plant physiology, growth and development, such as: water use efficiency, water retention = rate: improving nitrogen use efficiency, enhancing carbon assimilation, improving photosynthesis, improving germination efficiency and accelerating maturation . The harvest can be further improved by improving the plant structure (under pressure and pressure-free conditions), including (but not limited to): early flowering 'controlling flowering to produce hybrid seeds, seedling growth, plant size, knot = quantity and Distance, root growth, seed size, fruit size, fruit size, number of bells, number of seeds or seeds per ear, seed quality, strengthening, must-have 'salty' seeds scattered, reduced fruit pod cracking and lodging resistance Sex. Its = harvest characteristics include seed composition, such as: carbohydrate content, protein containing read composition, nutritional value, reducing undesirable nutrient compounds, improving processability and improving storage stability. Plants that can be treated by Changli Benming have been shown to have heterosis or hybrid plants, which generally improve yield, growth, health and resistance to fine factors. The typical method for these plants is to cross the parental parent (mother) and the other (parent). Hybrid seeds are typically transmitted to the male parent of the male parent. The paper is also recognized as a self-important plant, and then the 201006383 method is used to make the male determinant in the plant genome. At this time and especially when it is desired to harvest seeds from the hybrid plants as the desired product, it is generally applicable to ensure that the hybrid plants are completely restored to male sex. In order to ensure that the paternal parent has the appropriate purine recovery gene, the hybrid plant containing the genetic determinant responsible for male sex can be restored to male sex. The male gene determinant can be located in the cytoplasm. Examples of cytoplasmic and male convulsions (CMS) are described, for example, in the crucifer species (Brassica genus). However, the male gene determinant can also be located in the nuclear genome. Male plants can also be obtained using plant biotechnology methods such as genetic engineering. A particularly suitable method for obtaining a male plant is described in WO 89/10396, in which, for example, a ribonuclease such as: barn a s e is selectively expressed in the cell membrane of the stamen. Inhibition can be restored by ribonuclease inhibitors such as barstar in the expression of cystic cells. ~ Plant or plant cultivars (which can be obtained by plant biotechnology methods, such as genetic engineering methods) which can be treated according to the invention can be herbicide-tolerant plants, i.e. plants which are resistant to one or more specific herbicides. Such plants can be obtained by genetic transformation or by selecting a plant that contains tolerance to these herbicide tolerances. Herbicide-tolerant plants are, for example, glyphosate (glyph〇sateW-accepting plants', ie plants which can tolerate glyphosate herbicides or their salts. Plants can tolerate glyphosate through many different squares. For example : The herbicide-tolerant plant can be formed by encoding the enzyme 5-glycol acetonide oxalic acid _3_phosphoric acid synthase (EPS is called a gene transformed into a plant. Examples of such Epsps genes are Salmonella (9) The gene called 细菌γ〇α gene of bacteria (mutant strain -20- 201006383 CT7), cp4 gene of Agrobacterium (IV), bacteria encoding 倭倭 EPSPS, tomato EPSPS or goosegrass EPSPS (WO 2001/66704) It can also be a mutant EPSPS glyphosate-tolerant plant can also be obtained by gene expression of a glyphosate oxidoreductase enzyme. Glyphosate resistant plants can also be encoded by glyphosate The gene expression of the acetyltransferase enzyme is obtained. The glyphosate-tolerant plant can also be obtained by selecting a plant containing the natural mutation of the above gene.

其他除草劑抗性植物為例如：可以耐受會抑制酵素麩胺醯胺合成酶之除草劑，如：雙丙胺膦（bialaphos)、草銨膦 (ph—版—㈣或固殺草⑻論如叫之植物。此等植物製法為藉由表現脫除除草劑毒性之酵素或可抗拒該抑制作用之突變麵胺_合成_素而製得。其卜種脫除毒性之酵素為編碼草銨膦乙醯基轉化酶之酵素(如：纟自鏈黴菌 (streptomyees)屬種)之bar或⑽蛋白外性草錢膦乙《轉㈣之植物。 W現外源其他除草劑财受性植物亦為可以耐受會抑制經基 _，Kbg|(HppD)酵素之除草劑之植物々基二酶為催化對經基苯基丙綱酸醋(hpp)轉化成^尿酸 S曰之酵素。耐$ HPPD·抑·之植物可二床^ hppd酵素之基_編碼突變之HppD酵素之:=抗性對HPPD-抑制劑之耐受性之取得亦可藉由編：：。基因，來轉化植物，使其儘管在聊时二酵素之了物對刪抑制劑之耐受性除了猎由糾聊㈣受性料之制外，亦可藉由編碼預^ •21 - 201006383 酸鹽脫氫酶酵素之基因來轉化植物而改善。草劑抗性植物為可以耐受乙酿基乳酸鹽合成酶 =抑制劑之植物。已知之ALS-抑制劑包括例如：績酸基 Μ相類、三料啊類、錢基氧㈤苯甲酸醋類與/或％縣絲三姆__ (亦稱為乙«錄酸合成酶，AHAS)之不同突變可賦t 不同除草劑騎草雜社耐受性。已有讀㈣俩基腺類耐受性植物料㈣峨耐受性植物。其他輕琳嗣類对受性植物亦已有說明。其他確酿基腺類-與喃嗤琳_類财❹ 受性植物亦說明於例如：WO 2007/024782。其他耐受咪唑啉酮類與/或磺醯基脲類之植物製法為誘變法、在含有除草劑之細胞培養物中選拔或突變育種法如說明於例如：大豆、稻、製糖用甜菜、萵苣或葵花。亦可根據本發明處理之植物或植物栽培品種（由植物生物技術方法，如··遺傳工程法製得者)為昆蟲抗性基因轉殖植物，亦即有抗性對抗某些標乾昆蟲侵害之植物。此等植物可利用基因轉化法，或選拔包含賦與此等昆蟲抗性之突變之植❹ 物而得到。本文所採用”昆蟲-抗性基因轉殖植物”包括任何包含至少一種轉殖基因之植物，其中包含編碼下列各物之序列： 1)來自蘇雲金芽孢桿菌之殺昆蟲結晶蛋白質或其殺昆蟲部份’如：列於 Crickmore 等人之 Microbiology andOther herbicide-resistant plants are, for example, herbicides that can tolerate the inhibition of the enzyme glutamine indoleamine synthetase, such as: bialaphos, glufosinate (ph-plate-(four) or guava (8)) It is a plant which is prepared by an enzyme which exhibits the toxicity of herbicide removal or a mutant faceted amine which can resist the inhibition. The enzyme for removing toxicity is glufosinate-encoded. An enzyme of acetyl invertase (eg, from the genus Streptomyes) or (10) a protein of exogenous physico-phosphorylate "trans (IV). W is also exogenous to other herbicides. It can tolerate the conversion of phenyl thiocyanate (hpp) to uric acid S 曰 by the plant sulfhydryl dienzyme which inhibits the herbicide of the base _, Kbg|(HppD) enzyme. · The plant can be two beds ^ hppd enzyme base _ coding mutation HppD enzyme: = resistance to HPPD-inhibitor tolerance can also be obtained by editing:: Gene, to transform plants, so that Although in the chat, the enzymes of the two enzymes are resistant to the elimination of the inhibitors. It can also be improved by transforming plants by encoding the gene of pre-21 - 201006383 acid dehydrogenase enzyme. The grass-resistant plant is a plant which can tolerate the ethyl lactate synthase = inhibitor. ALS-inhibitors include, for example, the acid-based hydrazine phase, the three-material class, the ketone oxygen (5) benzoic acid vinegar and/or the % county sam __ (also known as B-acid synthase, AHAS) Different mutations can confer tolerance to different herbicides. It has been read (4) two gland-tolerant plant materials (4) tolerant plants. Other light amaranths have also been described for sexual plants. Other vegetative glands - and 嗤嗤 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Method, selection or mutation breeding in cell cultures containing herbicides as described, for example, in soybeans, rice, sugar beet, lettuce or sunflower. Plants or plant cultivars which may also be treated according to the invention (by plant biotechnology) Method, such as ·································· , that is, plants that are resistant to attack by certain dry insects. These plants can be obtained by genetic transformation or by selecting plantings containing mutations that confer resistance to these insects. A resistance gene transgenic plant" includes any plant comprising at least one transgene that comprises sequences encoding the following: 1) an insecticidal crystalline protein from Bacillus thuringiensis or an insecticidal portion thereof, eg, listed in Crickmore Microbiology and

Molecular Biology Reviews (1998)，62，807-813，由Molecular Biology Reviews (1998), 62, 807-813, by

Crickmore等人(2005)更新蘇雲金芽孢桿菌毒素命名 -22- 201006383 法，連線至：http : //WWW.lifeSci.SUSSex.ac.uk/H〇me/Neil—Crickmore/Bt/)之殺昆蟲結晶蛋白質，或其殺昆蟲部份，例如：Cry蛋白質類 Cry 1 Ab、Cry 1 Ac、Cry 1F、Cry2Ab、Cry3 Ae 或 Cry3Bb 或其殺昆蟲部份之蛋白質； 2) 來自蘇雲金芽孢桿菌之結晶蛋白質或其一部份，其在來自蘇雲金芽孢桿菌之第二種其他結晶蛋白質或其一部 ❿ 伤之存在下具有殺昆蟲性，如：由Cry34與Cry35結晶蛋白質組成之二元毒素；或 3) 包含來自蘇雲金芽孢桿菌之不同殺昆蟲結晶蛋白質之雜化殺昆蟲蛋白質，如：上述丨）之雜化蛋白質或上述2) 之雜化蛋白質，例如：由玉米品種M〇N98〇34產生之 CrylA.105蛋白質；或 4) 上述1)至3)中任一蛋白質，其中有些（特定言之丨至1〇種)胺基酸已被另一種胺基酸置換，可對標靶昆蟲屬種產 φ 生更高殺昆蟲活性且/或可擴大受影響之標靶昆蟲屬種範圍與/或在選殖或轉化期間，將變化引入編碼之DNA 中’如：玉米品種MON863或MON88017之Cry3Bbl 蛋白質，或玉米品種MIR604之Cry3A蛋白質；或 5) 來自穌雲金芽抱桿函或蝶質芽抱桿菌（方aczY/ws 之殺昆蟲性分泌蛋白質，或其殺昆蟲部份，如：有生長力之殺昆蟲（VIP)蛋白質，其列於：http : //www.lifesci.sussex.ac.uk/Home/Neil_Crickmore/Bt/vip. html，例如：來自VIP3Aa蛋白質類之蛋白質；或 -23· 201006383 6)來自蘇雲金芽孢桿菌或蠟質芽孢桿菌之分泌蛋白質，其在來自蘇雲金芽孢桿菌或蠟質芽孢桿菌之第二分泌^ 白質之存在下具有殺昆蟲性，如··由VIP1A與VIP2a 蛋白質組成之二元毒素；或一 7)包含來自蘇雲金芽孢桿菌或蠟質芽孢桿菌之不同分泌蛋白質之一部份之雜化殺昆蟲性蛋白質，如：上述U 之雜化蛋白質或上述2)之雜化蛋白質；或 8) 上述1)至3)中任一蛋白質，其中有些（特定言之1至Crickmore et al. (2005) updated the Bacillus thuringiensis toxin naming -22-201006383 method, connecting to: http: //WWW.lifeSci.SUSSex.ac.uk/H〇me/Neil-Crickmore/Bt/) a crystalline protein, or an insecticidal portion thereof, such as a Cry protein Cry 1 Ab, Cry 1 Ac, Cry 1F, Cry 2 Ab, Cry 3 Ae or Cry 3 Bb or an insecticidal portion thereof; 2) a crystalline protein from Bacillus thuringiensis Or a part thereof which is insecticidal in the presence of a second other crystalline protein from Bacillus thuringiensis or a smash thereof, such as a binary toxin consisting of Cry34 and Cry35 crystalline proteins; or 3) a hybrid insecticidal protein comprising a different insecticidal crystalline protein from Bacillus thuringiensis, such as: a hybrid protein of the above 丨) or a hybrid protein of the above 2), for example: CrylA produced by the maize variety M〇N98〇34. 105 protein; or 4) any of the above proteins 1) to 3), some of which (specifically, 1 to 1 species) of amino acids have been replaced by another amino acid, which can produce φ for target insect species Born to kill insects And/or may extend the range of affected target insect species and/or introduce changes into the encoded DNA during selection or transformation, such as: Cry3Bbl protein of maize variety MON863 or MON88017, or Cry3A protein of maize variety MIR604 Or 5) from the buds of the golden buds or the buds of Bacillus licheniformis (the insect-secreting protein of aczY/ws, or its insecticidal parts, such as the viable insecticidal (VIP) protein, It is listed at: http://www.lifesci.sussex.ac.uk/Home/Neil_Crickmore/Bt/vip.html, for example: protein from VIP3Aa protein; or -23· 201006383 6) from Bacillus thuringiensis or wax a secreted protein of Bacillus licheniformis that is insecticidal in the presence of a second secretory protein from Bacillus thuringiensis or Bacillus cereus, such as a binary toxin composed of VIP1A and VIP2a proteins; or a 7) a hybrid insecticidal protein comprising a portion of a different secreted protein from Bacillus thuringiensis or Bacillus cereus, such as: a hybrid protein of U above or a hybrid protein of 2); or 8) above 1) 3) according to any one of the proteins, some of which (1 to specific words

種)胺基酸已被另一種胺基酸置換’可對標靶昆蟲屬種羞士更高殺昆蟲活性且/或可擴大受影響之標靶昆蟲屬賴範圍與/或在選殖或轉化期間，將變化引入編碼之DNy 中（同時仍可編碼殺昆蟲性蛋白質），如： C〇T1〇2之VIP3Aa蛋白質。筛化口口卷含編踩？:昆蟲抗性基因轉殖植物當然亦包括任何包 ❹ 8類任—種蛋白f之基因組合之植物。-項 ς實施财’昆蟲抗性植物包含超過—種 8 =蛋白質之轉殖基因，當使用不同蛋白質針對不：使=3:可以擴大影響之標乾昆蟲屬種範圍，或藉由有m 5蛋白讀相同縣昆蟲屬種具有H蟲性，但卻且用模式’如：與③蟲中不同受體結合活性結合二延^昆蟲對植物發展出抗性。物括2根據本發明處理之植物或植物栽培品種（由植物生術方法，如·· ϋ傳工程法製得)可耐受非生物性壓力因 •24· 201006383 性突由=化法或選拔包含賦與峨力抗生大=植物“。特別有用之壓㈣受性植物包括： .，低聚(ADP_核糖）聚合酶(parp)基因在植物、二=中之表現與/或活性之轉殖基因之植物。 L3可以降低植物或植物細與/或活性之加強壓力耐勺2之PARG編碼基因之表現包含編碼於酿胺料殖基因之植物。 4, , A. LL A 7 —核苷酸回收生合成途徑之植轉化酶 /下7基钇化鉍、菸醯胺腺嘌呤二核苷酸 ;殖基二::胺核糖碟酸轉化酶)之加強壓力耐受性具有上述特性之植物無限制實例示於表A。子 b. c.The amino acid has been replaced by another amino acid' to be more insecticidal to the target insect species, and/or to expand the range of affected insects and/or to colonize or transform During this time, changes are introduced into the coded DNy (while still encoding insecticidal proteins), such as: VIP3Aa protein of C〇T1〇2. Screening mouth volume with braided tread? : Insect resistance gene transgenic plants of course also include any plant comprising a combination of 8 gene types of protein f. - Item ς 财 ' ' ' insect-resistant plants contain more than - 8 = protein transfer genes, when using different proteins for no: make = 3: can expand the range of affected insect species, or by having m 5 The insects of the same county are H insect-like, but they use the pattern 'such as: binding to different receptors in the three insects combined with the two-intensity insects to develop resistance to plants. The plant or plant cultivar (which is prepared by the plant biotechnology method, such as the ϋ 工程 engineering method) which can be treated according to the present invention can withstand abiotic stresses. • 24·201006383 Sexual excavation = chemical method or selection Gives 峨力抗生大=植物". Particularly useful pressure (4) Sex plants include: ., oligo (ADP_ribose) polymerase (parp) gene in plants, two = performance and / or activity of the transformation Plant of the gene L3 can reduce the plant and plant fineness and / or activity of the enhanced pressure resistance of the PARG-encoding gene of scoop 2 contains plants encoding the gene encoding the amine gene. 4, , A. LL A 7 - nucleotide Regeneration of the biosynthetic pathway of the plant invertase / the lower 7-base guanidine, nicotinamide adenine dinucleotide; the enzyme II:: amine ribose acid conversion enzyme) enhanced stress tolerance plants with the above characteristics The restricted examples are shown in Table A. Subbc

No. . A-lXT' $成之目標或表現之原作物表型/耐受性乙醯乳酸酯合成酶(ALS) 乙醯基C〇A羧化酶 ^ACCase) ---------- 羥基苯基丙S同酸酯二氧化酶(HPPD) $安膦乙醯酶基轉化gj 磺酿脲類、咪唑啉酮類、三唑并嘧啶類、氧苯曱酸酯類、苯酞類务基氧本氧基烧缓酸類、環己二I同類異口号唑類’如：異噚唑草酮(isoxaflutol) 或異吟氯_ (isoxachlortole)，三酮類，如··硝草酮（mes〇trione)或續草酮 (sulcotrione) 草 I安膦(phosphinotricin) 改變木質素含量 -25- 201006383 一 fill A-6 埂成之目標或表現之眉理 ·" -— 作物表型/财受性麵胺醯胺合成酶固殺草（Glufosinate)、雙丙胺膦| (Bialaphos) ------— IMP與AMP合成作用抑制劑 ~~~ JA-7 腺苷酸基琥珀酸裂解酶 (ADSL) A-8 ~~~-~~1----— 腺苷酸基琥珀酸合成酶 ——-- 腺苷酸基琥珀酸合成作用抑制劍 A-9 鄰胺基苯甲酸合成醢 -—---LJJ … 色胺酸合成作用與代謝作用;备丨滅丨 A-10 腈水解酶 3,5-二鹵-4-羥基-苯基氰，如：溴苯腈 (Bromoxynil)與艾辛尼(ioxin vn A-ll 5-烯醇丙酮醯基_3_麟代莽草酸酯合成酶(EPSPS) -------v V 草甘膦(Glyphosate)或草硫膦(suifosate) A-12 草甘鱗氧化還原酶草甘膦或草硫麟(sulfosate) A-13 原卟啉原氧化酶 (PROTOX) 二苯基醚類、環狀亞胺類、苯基„比唑類、 °比啶衍生物、樂撲草(phenopyiate)、哼二唑類，等等 A-14 細胞色素P450，例如： P450 SU1 異種生物劑與除草劑，如：磺醯脲類 A-15 Dimboa生合成作用（Bxl 基因）玉米炼故病菌（Helminthosporium turcicum)、玉米葉场⑻j〇pa丨〇s^um maydis)、玉米殼色二孢（Dip!〇dia maydis)、歐洲玉米螟（(¾的.論 nubihlis)、鳞翅吕慝祷 A-16 CMIII(小型鹼性玉米種子肽）植物病原囷例如：鐮抱菌仿、鍵格孢、菌核病菌扣化⑽/叫） A-17 玉米-SAFP(玉米蛋白質 (zeamatin)) 植物病原囷例如：錄抱菌诉如、鍵格抵(altemaria)、菌钱病窗(sclerotina)、絲核鹵（Hn’zoctowia)、毛殼菌㈣2、鬍鬚黴 -26- 201006383No. . A-lXT' $ is the target or performance of the original crop phenotype / tolerance acetate lactate synthase (ALS) acetonitrile C 〇 A carboxylase ^ ACCase) ------ ---- hydroxyphenyl propyl S and acid ester dioxygenase (HPPD) $ an phosphine acetamyl conversion gj sulfonate urea, imidazolinones, triazolopyrimidines, oxybenzoic acid esters, Benzoquinone-based oxy-oxygen oxy-acids, cycloheximide I-like singular azoles such as: isoxaflutol or isoxachlortole, triketones, such as Mesquiteone or sulcotrione phosphinotricin Change lignin content-25- 201006383 A fill A-6 The goal or performance of the · · · · · - Crop table Type/Acid-Producing Aminoguanamine Synthase Glufosinate, Bialaphos | (Bialaphos) ------ - IMP and AMP Synthetic Inhibitor ~~~ JA-7 Adenyl Amber Acid lyase (ADSL) A-8 ~~~-~~1----- adenyl succinate synthetase --- Adenyl succinic acid synthesis inhibits Sword A-9 o-Aminobenzene Synthesis of formic acid —-----LJJ ... synthesis of tryptophan Metabolism; preparation of cockroach A-10 nitrilase 3,5-dihalo-4-hydroxy-phenyl cyanide, such as: bromoxynil (Bromoxynil) and Essini (ioxin vn A-ll 5-enol Acetone sulfhydryl _3_ linalyl oxalate synthase (EPSPS) -------v V Glyphosate or sulphur sulphate (Sifosate) A-12 Glycyrrhizin oxidoreductase Phosphine or sulfosate A-13 protoporphyrinogen oxidase (PROTOX) diphenyl ethers, cyclic imines, phenyl pi-azoles, pyridine derivatives, phenopyiate ), oxadiazoles, etc. A-14 Cytochrome P450, for example: P450 SU1 xenobiotics and herbicides, such as: sulfonylureas A-15 Dimboa biosynthesis (Bxl gene) Corn liquefied bacteria (Helminthosporium) Turcicum), corn leaf field (8) j〇pa丨〇s^um maydis), corn husk spore (Dip! 〇dia maydis), European corn borer ((3⁄4. on nubihlis), lepidopteran Lv prayer A-16 CMIII (small alkaline corn seed peptide) Plant pathogens such as: Brassica, Brassica, Sclerotinia sclerotiorum (10) / called) A-17 Maize-SAFP (zeamatin) Plant pathogens such as: Recording bacteria, such as, for example, altemaria, sclerotina, Hn’zoctowia, Chaetomium (2) 2, Coriander -26- 201006383

Mil 達成之目標或表現之原 1理 ~~— — 作物表型/耐受性 A-18 A-19 Hml基因幾 ~ 菌(Cochliobulus) __^勿病原菌 A-20 葡聚醣酶植物病原菌 A-21 被膜蛋白質病毒，如：玉米矮化嵌紋病毒、玉米氯矮化病毒(com chlorotic dwarf virus) A-22 蘇雲金芽孢桿菌(方 thuringiensis)秦責、γιρ 3、仙人掌桿菌(5沉油《 cere㈣毒素、發光桿菌〇P/2〇ic»ra0<ii^ 與異桿菌 {Xenorhabdus)卷每~ 鱗翅目、鞘翅目、雙翅目、線蟲，例如：玉米填蛾(CtoWm’a 、玉米穗夜蛾(报/如/ώ 、黏蟲，例如：草地黏蟲XSpodoptera frugiperda)、玉米根轰、虫主莖夜蛾0¾犯、小地老虎、亞洲玉米螟、象鼻蟲 A-23 3-羥基類固醇氧化酶 — 鱗翅目、勒翅目、雙翅目、線蟲，例如：玉米模蛾、玉米穗夜蛾(Heliothis zea)、黏蟲，例如：草地黏 A(Spodoptera frugiperda)、玉米根螽、虫主莖夜蛾、小地老虎、亞州玉米螟、象鼻蟲 A-24 A-25 過氧化酶 ~~-- 鱗翅目、鞘翅目、雙翅目、線蟲，例如：玉米填蛾fQstnWa 、玉米穗夜蛾(i/e/如紙 zea；、黏蟲，例如：草地黏蟲(Spodoptera frugiperda)、玉米根蟲、虫主莖夜蛾、小地老虎、亞州玉米螟、象鼻蟲胺基狀姆抑制劑，例如：白月女酸胺基狀酶抑制劑 (LAPI) ----— 鱗翅目、鞘翅目、雙翅目、線蟲，例如：玉米填蛾、玉米穗夜蛾(7/e/z·况to 、黏蟲，例如：草地黏蟲(Spoifopiera /rwg/percfaj、玉米根蟲、 -27- 201006383 達成 - ^：Ν〇ιρι;: 理 ’、或表現之原作物表型/耐受性 ~~~~ 虹莖夜蛾(Sesamia sp_)、小地老虎、亞州 Α-26 -- _^歩螟、象鼻蟲 Α-27 外源凝- 米根蟲 ~~~~ 鱗翅目、鞘翅目、雙翅目、線於如.. 玉米填蛾((¾的>?ζ·α 、玉米棟夜蛾zey、黏蟲’例如：草地黏 A(Spodoptera frugipenia)、玉米板義、虫主莖夜蛾、小地老虎、亞州 Α-28 蛋白酶抑制劑，例如：半胱胺酸蛋白酶抑制劑、儲存性蛋白質抑制劑 (patatin)、硫醇蛋白酶抑制劑(virgiferin)、CPTT 玉米螟、象鼻蟲象鼻蟲、玉米根蟲 Α-29 核糖體去活化蛋白質鱗翅目、鞠翅目、雙翅目、線蟲，例如·· 玉米模蛾(〇·5ίη>7ώ m/Ma/句、玉米穗夜蛾zea)、黏蟲，例如：草地黏蟲XSpodoptera frugiperda)、玉米根轰、虫主莖夜蛾(5^α777Ζ·<3年」、小地老虎、亞州玉米螟、象鼻蟲 Α-30 玉米5C9-多肽鱗翅目、鞘翅目、雙翅目、線蟲，例如：玉米培蛾(Osfrinia nubi!a〖is)、玉米穩夜蛾zea)、黏蟲，例如：草地黏蟲(*S/?〇iioptera 由erc/α)、玉来根蟲、姓莖夜蛾(Skraw/a平j、小地老虎、亞州玉米螟、象鼻蟲 -28- 201006383Mil's goal or performance of the original 1 ~ ~ - crop phenotype / tolerance A-18 A-19 Hml gene ~ ~ bacteria (Cochliobulus) __^ pathogen A-20 glucanase plant pathogen A- 21 envelope protein virus, such as: corn dwarf mosaic virus, corn chlorotic dwarf virus A-22 Bacillus thuringiensis (square thuringiensis) Qin, γιρ 3, cactus bacillus (5 oil oil cere (four) toxin , Photobacterium 〇P/2〇ic»ra0<ii^ and Bacillus subtilis {Xenorhabdus) rolls per lepidopteran, coleoptera, diptera, nematodes, for example: corn-filled moth (CtoWm'a, corn snail ( Report / such as / ώ, sticky insects, such as: grass worm XSpodoptera frugiperda), corn root blast, worm main moth, 3⁄4 guilt, small tiger, Asian corn borer, weevil A-23 3-hydroxy steroid oxidase - Lepidoptera, Lepidoptera, Diptera, Nematodes, for example: Maize moth, Heliothis zea, Zygomycetes, for example: Spodoptera frugiperda, Corn root stalk, worm main stem night Moth, small tiger, Asian corn borer, weevil A-24 A-25 Peroxidase~~-- Lepidoptera, Coleoptera, Diptera, Nematodes, for example: corn-filled moth fQstnWa, Spodoptera frugiperda (i/e/ as paper zea;, armyworm, eg: grass worm (Spodoptera) Frugiperda), corn rootworm, larvae, larvae, larvae, larvae, nymphalamine-based inhibitors, such as: leucovorin-based enzyme inhibitor (LAPI) --- - Lepidoptera, Coleoptera, Diptera, Nematodes, for example: corn-filled moth, Spodoptera frugiperda (7/e/z, tomy, for example: grass worm (Spoifopiera /rwg/percfaj, corn) Rootworm, -27- 201006383 Reached - ^:Ν〇ιρι;: The original crop phenotype/tolerance of ', or performance~~~~ Sesamia sp_, small tiger, Asian Α -26 -- _^歩螟, weevil Α-27 exogenous coagulation - rice rootworm ~~~~ Lepidoptera, Coleoptera, Diptera, and line such as: corn-filled moth ((3⁄4的> ζ·α, zey, and wormy ', such as: Spodoptera frugipenia, corn stalk, larvae, snail, Asian Α-28 protease inhibitor, Such as: cysteine protease inhibitor, statin protein inhibitor (patatin), thiol protease inhibitor (virgiferin), CPTT corn borer, weevil weevil, corn rootworm Α-29 ribosome deactivated protein Lepidoptera, Lepidoptera, Diptera, nematodes, for example, maize moth (〇·5ίη>7ώ m/Ma/sentence, zea zea), armyworm, for example: XSpodoptera frugiperda) Corn root blast, worm main moth (5^α777Ζ·<3 years), small tiger, Asian corn borer, weevil Α-30 maize 5C9-polypeptide lepidoptera, coleoptera, diptera, nematode For example: corn moth (Osfrinia nubi! a 〖is), corn worm (zea), armyworm, for example: grass worm (*S/?〇iioptera by erc/α), jade rootworm, surname stem Noctuidae (Skraw/a flat j, small tiger, Asian corn borer, weevil -28- 201006383

' ---- !_]!: 達成之目標或表現之原理作物表型/对受性 A-31 HMG-CoA還原酶鱗翅目、鞘翅目、雙翅目、線蟲，例如：玉米煩蛾「(〇价加/<3 77wMa/句、玉米科夜蛾zeflj、黏蟲，例如：草地勒蟲{Spodoptera frugiperda)、玉朱极备、虫主莖夜蛾切」、小地老皮、亞州 A-32 --- 玉米螟、象鼻蟲抑制蛋白質合成作用 — 氯乙醯替苯胺類，如：拉草(Alachlor)、乙草胺(Acetochlor)、太 A、33 ---- --— 草滅(Dimethenamid) 疑似劑 2,4-D、滅克普(Mecoprop)-P 物括亦可根據本發明處理之植物或植物栽培品種（由植物生量、法，如：遺傳工程法製得）所收成產品展現改良之產貝與7或儲存安定性且/或改變所收成產品特定成份之 )合成改質澱粉之基因轉殖植物，其相較於野生種植物細月己或植物所合成殿粉，已在物化特性上，特定言之，直鏈凝粉含量或直鏈澱粉/支鏈澱粉比例、分支程度、平均鏈長、側鏈分佈、黏度表現、膠凝強度、澱粉粒大小與 2) ^歟粉粒形態等方面均已改變，因此更適於特定用途。合成非殿粉碳水化合物聚合物或合成相較於未經基因改4之野生種植物具有改變之性質之非殿粉碳水化合物聚合物之基因轉殖植物。其實例為產生聚果糖之植物’尤指菊糖型與果聚糖型、產生ad〆葡聚糖之植物、 -29- 201006383 產生α-1，6分支α-1，4葡聚糖之植物、產生alternan之植物。 3) 產生玻尿酸(hyaluronan)之基因轉殖植物。特別適合根據本發明處理之基因轉殖植物為包含轉化結果或包含轉化結果組合之植物，其係在美國向美國農部 (the United States Department of Agriculture(USDA))之動物與植物健康檢查局（the Animal and Plant Health Inspection Service(APHIS))申請非管制狀態之主題，不論此等申請案是否已核准或仍在審核中。任何時間均可自APHIS(4700River RoadRiverdale，MD 20737，USA)取得此訊息，例如：取自網際網路(URL http : //www.aphis.usda.gov/brs/not_reg.html)。在本申請案申請曰時’該等仍在APHIS審核中或已由APHIS 核准之非管制狀態之申請案已列於表B ’其中包含下列資訊：申請案：申請案檔案編號。轉化結果之技術說明可參見自APHIS取得之各申凊文獻，例如：aphis網站，參考此申請案編號。此等說明内容已以引用方式併入本文中。延伸申請案：提及要求延伸之先前申請案。〇機構：提交申請案之機構名稱。管制項目：相關植物屬種。轉殖基因表型.因轉化結果所賦與植物之特性。轉化結果或品系：要求非管制狀態之轉化結果名稱(有時候稱為品系）。八聰文獻1 APHIS公開與該申請案相關之各種文獻，且可依據APHIS之請求。 20e« < 預備EA**** 或風險評估 06-27 l-01p_pea 1 FR通知 5-Oct-2007 轉化結果或品系 MIR-162 玉米 IFD-19890-1 與 IFD-19907-9 HT-98140 COT67B DP-305423-1 GHB614 ΜΟΝ 89034 356043 轉殖基因表型改變花色草甘膦與味。坐琳酮類耐受型鱗翅目抗性型高油酸草甘膦耐受型歐洲玉米螟抗性型草甘膦與乙醯乳酸管制項目康乃馨玉米棉花大豆棉花玉米大豆機構新基塔 (Syngenta) 佛林 (Florigene) 先鋒 (Pioneer) 新基塔 (Syngenta) 先鋒 (Pioneer) # δ .2 Η- β & 故齋Ο 孟山都 (Monsanto) 先鋒 (Pioneer) 延伸申請案編號*** 申請案 07-253-01p 07-180-0lp 07-152-0 lp 07-108-01p 06-354-01p 06-332-01ρ 06-298-0 lp 06-27 l-01p ώ B-2 rn ώ B-4 ώ Β-6 B-7 B-8 Με- 201006383 < Η i： ε ο s cp ό 1 預備EA*AAA 或風險評估 04-110-01p_pea cd _ Is s铼 FR通知 g in …寸 >5 ο ο o cq o o o ji (N (N (N ^ 'Ο (N ^ 芩A A 1 rn 卜寸 (N (N Ο (N 範圍與狀態； 12-0ct-2005 ； 11.04.2005 ； 18.11.2004 ； 24.09.2004 ； 05.01.2004 轉化結果或品系 3272 XI7-2 J101、J163 ASR368 轉殖基因表型酯合成酶耐受型熱安定性α -澱粉酶木瓜環點病毒抗性型草甘膦财受型草甘膦耐受型管制項目呆木瓜小糠草機構新基塔 (Syngenta) 佛羅里達大學 i砩 ^ f | I | ^ u2举旧 2 e ^ 〇孟山都 (Monsanto) 與史考特 (Scotts) 延伸申請案編號*** 申請案 05-280-01p 04-337-Olp 04-110-01ρ 03-104-01p OS ώ B-10 Β-11 B-12' ---- !_]!: The goal of achieving the goal or the performance of the crop phenotype / on the A-31 HMG-CoA reductase Lepidoptera, Coleoptera, Diptera, nematodes, such as: corn moth (〇价 plus /<3 77wMa/sentence, corn worm zeflj, armyworm, for example: stalk worm {Spodoptera frugiperda), jade sylvestris, snail main stalk moth, small land old skin, ya State A-32 --- Corn borer, weevil inhibits protein synthesis - chlorhexidine aniline, such as: Alachlor, Acetochlor, A, 33 ---- -- - Dimethenamid suspects 2,4-D, Mecoprop-P include plants or plant cultivars that can also be treated according to the invention (produced by plant yield, method, eg genetic engineering) a genetically modified plant of synthetically modified starch that exhibits an improved yield of shellfish and 7 or storage stability and/or a specific component of the harvested product, which is synthesized in comparison with wild plants or plants. Dian powder, has been in the physical and chemical properties, specifically, the linear coagulum content or amylose / amylopectin ratio, branch The average chain length, the side chain distribution, the viscosity performance, the gelling strength, the starch grain size and aspect 2) ^ morphology particles have been striving to change, and therefore more suited to the particular use. Synthetic non-house powder carbohydrate polymer or a genetically transgenic plant that synthesizes a non-dural powder carbohydrate polymer having altered properties compared to a wild plant that has not been genetically modified. Examples thereof are plants which produce polyfructose, especially plants which are inulin type and fructan type, which produce ad dextran, -29-201006383 plants which produce α-1,6 branches α-1,4 glucan , the plant that produces alternan. 3) Geneogenic plants that produce hyaluronan. A genetically modified plant that is particularly suitable for treatment according to the present invention is a plant comprising a transformation result or a combination comprising a transformation result, which is in the United States to the United States Department of Agriculture (USDA) Animal and Plant Health Inspection Service ( The Animal and Plant Health Inspection Service (APHIS) applies for the subject matter of non-regulated status, whether or not such applications have been approved or are still under review. This information can be obtained from APHIS (4700 River Road Riverdale, MD 20737, USA) at any time, for example, from the Internet (URL http://www.aphis.usda.gov/brs/not_reg.html). At the time of the application of this application, the applications for such non-regulated status that are still under APHIS audit or approved by APHIS are listed in Table B' which contains the following information: Application: Application file number. The technical description of the conversion results can be found in the various documents obtained from APHIS, for example: aphis website, refer to the application number. These descriptions are incorporated herein by reference. Extended application: refers to the previous application that requires extension.机构 Organization: The name of the institution submitting the application. Control project: related plant species. Transgenic gene phenotype. The characteristics of the plant are attributed to the transformation results. Conversion result or line: The name of the conversion result (sometimes called a line) that requires an unregulated status. Ba Cong Literature 1 APHIS discloses various documents related to this application and can be based on the request of APHIS. 20e« < Preparation EA**** or Risk Assessment 06-27 l-01p_pea 1 FR Notification 5-Oct-2007 Conversion Results or Lines MIR-162 Maize IFD-19890-1 and IFD-19907-9 HT-98140 COT67B DP-305423-1 GHB614 ΜΟΝ 89034 356043 Transgenic gene phenotype changes the color of glyphosate and taste. Selenone-tolerant lepidopteran-resistant high oleic acid glyphosate-tolerant European corn borer resistant glyphosate and acetonitrile lactate control project carnation corn cotton soybean cotton corn soybean plant new base (Syngenta) Florigene Pioneer Syngenta Pioneer # δ .2 Η- β & 斋Ο Monsanto Pioneer Extension Application No. *** Application 07- 253-01p 07-180-0lp 07-152-0 lp 07-108-01p 06-354-01p 06-332-01ρ 06-298-0 lp 06-27 l-01p ώ B-2 rn ώ B-4 ώ Β-6 B-7 B-8 Με- 201006383 < Η i: ε ο s cp ό 1 Prepare EA*AAA or risk assessment 04-110-01p_pea cd _ Is s铼FR notification g in ... inch >5 ο ο o cq ooo ji (N (N ^ N Ο Ο (N ^ 芩AA 1 rn 卜 inch (N (N N (N range and state; 12-0ct-2005; 11.04.2005; 18.11.2004; 24.09 .2004 ; 05.01.2004 Transformation results or lines 3272 XI7-2 J101, J163 ASR368 Transgenic gene phenotype ester synthase tolerant heat-stable α-amylase papaya ring virus resistant Glyphosate-tolerant glyphosate-tolerant control project, papaya, sorghum, new plant (Syngenta), University of Florida, i砩^ f | I | ^ u2, old 2 e ^ Monsanto and Scotts ) Extension Application No. *** Application 05-280-01p 04-337-Olp 04-110-01ρ 03-104-01p OS ώ B-10 Β-11 B-12

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20 3 8 3 6 ® < Μ t Μ 06-234-0 lp—com 06-178-0 lp_com 04-362-01p—com 04-264-0 lp_com 04-229-0 lp—com 04-125-01p_com 04_086-01p一 com 03-353-01p一 com 預備EA**** 或風險評估 06-234-0 lp_pea 06-178-0 lpjpea 04-362-0 lp_pea 04-264-0 lp_pea 04-229-0 lp_pea 04-125-0lp_pea 04-086-01pj)ea 03-353-0 lp_pea FR通知 4-Dec-2006 ； 08.09.2006 02.08.2007 ； 05.02.2007 23-Mar-2007 f 22.02.2007 ； 10.01.2007 13-July-2007 ， 16-May-2006 03.02.2006 ； 27.09.2005 06.01.2006 ； 12.08.2005 03.01.2005 ； 24.11.2004 ； 4-Oct-2004 07.08.2005 ；轉化結果或品系 LLRICE601 MON 89788 MIR604 LY038 88017 MON 88913 59122 轉殖基因表型草銨膦耐受型草甘膦耐受型抗玉米根蟲李子痘病毒抗性型高離胺酸玉米根蟲抗性型草甘膦耐受型玉米根蟲 1 管制項目 m 大豆玉米屮 νή 棉花玉米機構龚S .2 Η- 6 & 紫郝U 孟山都 (Monsanto) 新基塔 (Syngenta) ARS 孟山都 (Monsanto) 孟山都 (Monsanto) 孟山都 (Monsanto) 道氏(Dow) 延伸申請案編號*** 98-329-Olp 申請案 06-234-Olp 06-178-01p 04-362-0 lp 04-264-0 lp 04-229-01p 04-125-Olp 04-086-01p 03-353-01p B-13 B-14 B-15 B-16 B-17 B-18 B-19 B-20 -ε£- 201006383 ίύ 欢 03-323-0 lp_com 03-181-01p_com 03-155-0 lp_com 03-036-01p_com 03-036-02p_com 02-042-01p_com 01-324-0 lp_com 預備EA**** 或風險評估 03-323-01pjpea 03-181-01p_pea 03-155-0 lp_pea 03-036-0 lp_pea 03-036-02p_pea FR通知 01.07.2005 17-Mar-2005 ；19-0ct-2004 01.11.2004 ； 17.08.2004 20.07.2005 ； 28.01.2005 13.08.2004 ； 9-Mar-2004 _ 13.08.20049- Mar-2004 轉化結果或品系 H7-1 TC-6275 COT 102 281-24-236 _ 3006-210-23 _1 LLCotton25 ! _1 RT200 ] 1 轉殖基因表型抗性型草甘膦耐受型鱗翅目抗性型與草敍膦对受型鱗翅目抗性型鱗翅目抗性型鱗翅目抗性型草銨磷耐受型草甘膦耐受型管制項目糖用甜菜玉米棉花棉花棉花棉花 * 機構孟山都 (Monsanto) 道氏(Dow) 新基塔 (Syngenta) 麥克金 (Mycogen)/ 道氏(Dow) 麥克金 (Mycogen)/ 道氏(Dow) 亞文提斯 (Aventis) 丨孟山都 (Monsanto)丨延伸申請案編號*** 00-136-01p 98-216-Olp 申請案 03-323-Olp 03-181-Olp —— 1 03-155-01p 03-036-0 lp 03-036-02p . 02-042-Olp …1 01-324-0lp 1 B-21 B-22 -, B-23 1 B-24 B-25 1 B-26 1 B-27 _120 3 8 3 6 ® < Μ t Μ 06-234-0 lp—com 06-178-0 lp_com 04-362-01p—com 04-264-0 lp_com 04-229-0 lp—com 04-125- 01p_com 04_086-01p a com 03-353-01p a com preparation EA**** or risk assessment 06-234-0 lp_pea 06-178-0 lpjpea 04-362-0 lp_pea 04-264-0 lp_pea 04-229- 0 lp_pea 04-125-0lp_pea 04-086-01pj)ea 03-353-0 lp_pea FR Notice 4-Dec-2006 ; 08.09.2006 02.08.2007 ; 05.02.2007 23-Mar-2007 f 22.02.2007 ; 10.01. 2007 13-July-2007, 16-May-2006 03.02.2006; 27.09.2005 06.01.2006; 12.08.2005 03.01.2005; 24.11.2004; 4-Oct-2004 07.08.2005; Conversion result or strain LLRICE601 MON 89788 MIR604 LY038 88017 MON 88913 59122 Transgenic gene phenotype glufosinate-tolerant glyphosate-tolerant anti-corn rootworm plum-pox virus-resistant high-alkaline corn-root-resistant glyphosate-tolerant maize Rootworm 1 Control Project m Soybean Corn 屮νή Cotton Corn Institute Gong S.2 Η- 6 & Purple Hao U Monsanto New Synta (Austragenta) ARS Monsanto Monsanto Monsanto Monsanto Monsanto (Monsanto) Dow Extension Application No. *** 98-329-Olp Application 06-234-Olp 06-178-01p 04-362-0 lp 04-264-0 lp 04-229-01p 04 -125-Olp 04-086-01p 03-353-01p B-13 B-14 B-15 B-16 B-17 B-18 B-19 B-20 -ε£- 201006383 ίύ Huan 03-323-0 Lp_com 03-181-01p_com 03-155-0 lp_com 03-036-01p_com 03-036-02p_com 02-042-01p_com 01-324-0 lp_com Prepared EA**** or Risk Assessment 03-323-01pjpea 03-181 -01p_pea 03-155-0 lp_pea 03-036-0 lp_pea 03-036-02p_pea FR Notice 01.07.2005 17-Mar-2005 ;19-0ct-2004 01.11.2004 ; 17.08.2004 20.07.2005 ; 28.01.2005 13.08 .2004 ; 9-Mar-2004 _ 13.08.20049- Mar-2004 Conversion result or strain H7-1 TC-6275 COT 102 281-24-236 _ 3006-210-23 _1 LLCotton25 ! _1 RT200 ] 1 Transgenic gene list Type-resistant glyphosate-tolerant lepidopteran-resistant and glufosinate-resistant glyphosate-tolerant lepidopteran-resistant lepidopteran-resistant lepidopteran Project Sugar Beet Corn Cotton Cotton Cotton Cotton * Agency Monsanto (Monsanto ) Dow New Syngenta Mycogen / Dow Mycogen / Dow Aventis san Monsanto 丨 Extension Application No. *** 00-136-01p 98-216-Olp Application 03-323-Olp 03-181-Olp —— 1 03-155-01p 03-036-0 lp 03-036-02p . 02-042- Olp ...1 01-324-0lp 1 B-21 B-22 -, B-23 1 B-24 B-25 1 B-26 1 B-27 _1

201006383 欢 Μ 01_206_01ρ一com 1 .. 01-206-02p_com 01-137-01p_com 01-121-01p_com 00-342-0 lp_com 00-136-01p_com 00-011-0 lp_com 99-173-0 lp_com 預備或風險評估 FR通知轉化結果或品系 MSI與 RF1/RP2 Topas 19/2 MON 863 Vector 21-41 棉化品系 15985 品系1507 NK603 _ RBMT22-82 轉殖基因表型草銨膦耐受型與控制授粉草銨膦耐受型玉未根蟲抗性型降低尼古丁鱗翅目抗性型鱗翅目抗性型草銨膦耐受型草甘膦耐受型 PLRV 與 CPB抗性管制項目油菜籽油菜籽玉米許棉花玉米玉米馬鈴薯機構亞文提斯 (Aventis) 亞文提斯 (Aventis) 孟山都 (Monsanto) 威克(Vector) 孟山都 (Monsanto) 麥克金 (Mycogen) c/o道氏 (Dow)與先鋒 (Pioneer) 孟山都 (Monsanto) 孟山都 (Monsanto) 延伸申請案編號*** 98-278-01p 97-205-Olp 97-099-01p 97-204-0 lp 申請案 01-206-01p 01-206-02p 01-137-01p Ol-121-Olp 00-342-0 lp 00-136-0lp 00-011-01p 99-173-01p B-28 B-29 B-30 B-31 B-32 B-33 B-34 B-35 201006383 1： < Μ 欢 Μ 98-349-0 lp_com 98-335-01p—com 98-329-0 lp_com 98-278-0 lp一com 98-23 8-0 lp_com 98-216-0 lp_com 98-173-01p_com 1 預備ΕΑ**ΑΑ 或風險評估 FR通知轉化結果或品系 MS6 CDC三裂 (Triffid) LLRICE06、 LLRICE62 MS8 與 RF3 GU262 RT73 GTSB77 轉殖基因表型草銨膦耐受型與雄不稔而亡受土壤殘留之磺醯脲類除草劑草銨膦耐受型草銨膦耐受型與控制授粉草銨膦耐受型草甘膦耐受型草甘膦耐受型管制項目亞麻梁油菜籽大豆油菜籽甜菜機構艾克伊弗 (AgrEvo) 由画中〇 J ^ ^ 艾克伊弗 (AgrEvo) 艾克伊弗 (AgrEvo) 艾克伊弗 (AgrEvo) 孟山都 (Monsanto) Λ w -3 Sim 5 t ? 铖屮$ 延伸申請案編號*** 95-228-01p 申請案 98-349-01p 98-335-Olp 98-329-0 lp 98-278-Olp 98-238-Olp 98-216-01p 98-173-01p B-36 B-37 B-38 B-39 B-40 B-41 B-42201006383 欢Μ 01_206_01ρ一com 1 .. 01-206-02p_com 01-137-01p_com 01-121-01p_com 00-342-0 lp_com 00-136-01p_com 00-011-0 lp_com 99-173-0 lp_com Preparation or risk Evaluation of FR Notification Transformation Results or Lines MSI and RF1/RP2 Topas 19/2 MON 863 Vector 21-41 Cotton Line 15985 Line 1507 NK603 _ RBMT22-82 Transgenic Gene Phenotype Glufosinate Tolerance and Control Pollination of Glufosinate Tolerant Jade Rootworm Resistant Reducing Nicotine Lepidopteran Resistant Lepidopteran Resistant Glufosinate Tolerant Glyphosate Tolerant PLRV and CPB Resistance Control Project Rapeseed Rapeseed Corn Hume Cotton Corn Potato plant Aventis Aventis Monsanto Vector Monsanto Mycogen c/o Dow and Pioneer Monsanto ) Monsanto Extension Application No. *** 98-278-01p 97-205-Olp 97-099-01p 97-204-0 lp Application 01-206-01p 01-206-02p 01-137-01p Ol-121-Olp 00-342-0 lp 00-136-0lp 00-011-01p 99-173-01p B-28 B-29 B-30 B-31 B-32 B-33 B-34 B-35 201006383 1: < Μ Μ 98-349-0 lp_com 98-335-01p—com 98-329-0 lp_com 98- 278-0 lp-com 98-23 8-0 lp_com 98-216-0 lp_com 98-173-01p_com 1 Preparation ΕΑ ΑΑ or risk assessment FR notification conversion results or strain MS6 CDC Triflation (Triffid) LLRICE06, LLRICE62 MS8 And RF3 GU262 RT73 GTSB77 transgenic phenotype glufosinate-tolerant and sulphonic sulfonamide herbicide glufosinate-tolerant glufosinate-tolerant and controlled pollination of glufosinate Tolerant glyphosate-tolerant glyphosate-tolerant control project Flax beam rapeseed soybean rapeseed beet plant Aike Evo (AgrEvo) by painting ^J ^ ^ Aike Evo (AgrEvo) Aike AgrEvo AgrEvo Monsanto Λ w -3 Sim 5 t ? 铖屮$ Extended Application No. *** 95-228-01p Application 98-349-01p 98-335- Olp 98-329-0 lp 98-278-Olp 98-238-Olp 98-216-01p 98-173-01p B-36 B-37 B-38 B-39 B-40 B-41 B-42

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201006383 e ❿ < Μ 欢 Μ 98-014-01p_com 97-342-01p_com a ο s 9 0's cn cn 97-336-0 lp_com 97-287-01p_com ε ο ο ν〇 CNJ ι> 97-205-0 lp_com 無域 FR通知 1_ 轉化結果 | 或品系 A5547-127 676、678、680 RBMT15-101 、 SEMT15-02、 SEMT15-15 T-120-7 5345 CBH-351 T45 轉殖基因表型草銨膦耐受型雄不稔與草銨膦耐受型 CPB與 PVY抗性型草銨膦耐受型鱗翅目抗性型草銨膦耐受型與鱗翅目抗性型草銨膦耐管制項目大豆玉米馬鈴薯甜菜玉米油菜籽機構都 (Monsanto) 艾克伊弗 (AgrEvo) 先鋒 (Pioneer) 孟山都 (Monsanto) 艾克伊弗 (AgrEvo) 孟山都 (Monsanto) 艾克伊弗 (AgrEvo) 艾克伊弗延伸申請案編號*** 96-068-01p 申請案 1_ 98-014-01p 97-342-0 lp 97-339-01p 97-336-01p 97-287-01p _i 97-265-01p 97-205-Olp B-43 B-44 B-45 B-46 _1 B-47 _ί B-48 1 _1 B-49 20 3 8 3 6 < 最終EA與判決ί 1_C 97-204-0 lp—com 97-148-0 lp_com 97-099-0 lp_com 97-013-01p_com 97-008_01p一com 96-317-0 lp_com 預備EA**** 或風險評估 FR通知轉化結果或品系 RBMT21-129 與 RBMT21-350 RM3-3 ' RM3-4、 RM3-6 GA21 31807 與 31808品系 G94-1 ' G94-19、 G-168 MON802 轉殖基因表型受型 CPB與 PLRV 抗性型雄不稔草甘膦耐受型溴苯腈耐受型與鱗翅目抗性型改變油型態草甘膦耐受型與 ECB抗性型管制項目馬鈴薯寐玉米棉花大豆玉米機構 (AgrEvo) 孟山都 (Monsanto) 比邱(Bejo) 孟山都 (Monsanto) 卡爾金 (Calgene) 杜邦〇Du Pont) 孟山都 (Monsanto) 延伸申請案編號*** 申請案 97-204-0 lp 97-148-Olp 97-099-0 lp 97-013-Olp 97-008-01p 96-317-Olp B-50 B-51 B-52 B-53 B-54 B-55 20 3 8 3 6 < t a 〇 S 〇 τ-^ 〇 <Ν ο B ο υι α, cp 〇〇 1 Ό OS 96-068-0 lp-C〇m 96-05 l-01p_com 96-017-01 p一 com a O o a1 cp (N Ο 95-338-01p一 com 預備EA*㈣或風險評估 FR通知 1_ 轉化結果或品系 DBT418 1株外加 FLAVRSAVR 品系 t 00 ' t 5 CN —的 ^ r-K CN CO , rf 4 r·^ N 〇〇寸 S 卜卜 m <N <N 们 ^ < 55-1 > 63-1 On Ο O i—· 00 00 ο ο CZW-3 V〇 ^ g έ ^ CQ _ <N H ^ o 卜 H , <N 00 ^ ^ 轉殖基因表型歐洲玉米螟抗性型改變果實成熟草銨膦耐受型 PRSV抗性型歐洲玉米螟抗性型 CMV、 ZYMV、 WMV2 抗性型 CPB抗性型管制項目玉米番莊大豆木瓜玉米南瓜馬鈴薯 | 機構 1_ 迪卡 (DeKalb) 卡爾金 (Calgene) 艾克伊弗 (AgrEvo) 康乃爾大學 (Cornell U) 孟山都 (Monsanto) 阿斯果 (Asgrow) 孟山都 (Monsanto) 1 延伸申請案編號*** 92-196-Olp 95-093-01p 申請案 96-291-01p 96-248-01p 96-068-0 lp 96-05l-01p 96-017-01p 95-352-01p . 1 _ _1 95-338-01p B-56 B-57 B-58 B-59 B-60 B-61 _______1 B-62 -6ε· 最終EA與判決 1_ 95-324-0 lp_com 95-256-01p—com 95-228-01p_com 95-195-0 lp_com 95-179-01p一 com 95-145-01p—com 95-093-01p_com 95-053-01p_com 银備EA**** 或風險評估 FR通知轉化結果或品系 rn cn 35 IN 19-51a MS3 Btll 2株外加 FLAVRSAVR 品系 B16 MON 80100 _1 8338 轉殖基因表型改變果實成熟磺醯脲類耐受型雄不稔歐洲玉米螟抗性型改變果實成熟草銨膦耐受型鱗翅目抗性型改變果實管制項目 N/ny 棉彳έ 玉米玉米玉米玉米機構埃利特 (Agritope) 杜邦(；Du Pont) 植物遺傳系統(Plant Genetic Systems) 諾特金 (Northrup King) 卡爾金 (Calgene) 迪卡 (DeKalb) 孟山都 (Monsanto) 孟山都延伸申請案編號*** 92-196-Olp 申請案 95-324-Olp ... 95-256-0 lp 95-228-01p 95-195-01p 95-179-01p 95-145-01p 95-093-01p 95-053-01p B-63 B-64 B-65 B-66 B-67 B-68 B-69 B-70 m < 95-045-0 lp一 com 95-030-01p 一com 94-357-01p一 com 94-319-01 p一 com 94-308-01 p一 com 94-290-0 lp_com 94-257-0 lp一 com 預備EA磁* 或風險評估 cd <ϋ cp in <N FR通知 10-Mar-1995 轉化結果或品系 1445 、 1698 20株外加 FLAVRSAVR 品系 T14、T25 品系176 _1 531、757、1076 f cd Q f BT6、BT10、 BT12、BT16、 BT17、BT18、 BT23 轉殖基因表型成熟草甘膦耐受型改變果實成熟草銨膦耐受型鱗翅目抗性型鱗翅目抗性型降低果實聚半乳糖醛酸酶含量鞠'翅目抗性型管制項目棉花 •WD diso 1 玉米玉米棉花 ψ 馬鈴薯機構 (Monsanto) 孟山都 (Monsanto) 卡爾金 (Calgene) 艾克伊弗 (AgrEvo) 希巴種子 (Ciba Seeds) 孟山都 (Monsanto) 4 ·§ _ € S « +屮i $ ㈤緦g 2 孟山都 (Monsanto) 延伸申請案編號*** 92-196-0lp 申請案 95-045-01p 95-030-01p 94-357-01p 94-319-0lp 94-308-01p 94-290-0 lp 94-257-01p B-71 B-72 B-73 B-74 B-75 B-76 B-77 < Μ t: 94-230-0 lp_com ( 94-228-01p_com 94-227-0 lp_com 94-090-0 lp—com 93-258Olp_com 93_196_01p_com 92-204-0 lpcom 92· 196-0 lp—com 預備或風險評估 94-228-0 lp_ea 92-204-0 lp_ea _ FR通知 24. Jan 95 3-Oct-1994 22. Feb 94 13-Dec-1994 19-Oct-1992 轉化結果或品系 9株外加 FLAVRSAVR 品系 1345-4 品系N73 1436-111 pCGN3828-21 2/86-18 與 23 40-3-2 BXN ZW-20 FLAVR SAVR 轉殖基因表型改變果實成熟改變果實成熟改變果實成熟改變油型態草甘膦耐受型溴笨腈耐受型 WMV2 與 ZYMV 抗性型改變果實成熟管制項目 .•iftn 4.D .4fm .•i/m MV» 大豆棉花南瓜機構卡爾金 (Calgene) DNA植物技術(DNA Plant Tech) 卡爾金 (Calgene) 卡爾金 (Calgene) 孟山都 (Monsanto) 卡爾金 (Calgene) 普強 (Upjohn) 卡爾金 (Calgene) 延伸申請案編號*** 92-196-0 lp 92-196-01p 申請案 94-230-0 lp 94-228-0 lp 94-227-01p 94-090-0 lp 93-258-Olp 93-196-01p 92-204-0 lp 92-196-0lp B-78 B-79 B-80 B-81 B-82 B-83 B-84 B-85 201006383 、根據本發明，抑丹尼(is〇tianil)可調配成各種不同製劑型 41明確言之’其實例包括可濕化粉劑、粒狀之可濕化粉劑、水'谷=粉劑、液體製劑、AL製劑、水懸劑及微膠囊製劑。亦可此使用包覆種子之製劑。此等製劑可依據已知方法製 _ 15 20 二及尼與展開劑（如：液體或固體稀釋劑或載人。告時’與界面活性劑（如：乳化劑與/或勻散劑）混 :溶;使用水作為展開劑時，可使用例如：有機溶劑作為辅 =體稀釋或载劑包括例如··芳香烴⑽如：二曱苯、甲笨烯類)氯化芳香烴或氯化脂烴類(例如：氣苯、氣化乙甲烷）、脂烴類[例如：環己烷，及鏈烷烴(例如：石，刀顧物)]、醇類（例如： _類(例如：丙_、¥其7其帕^及祕類，、具自曰類） ^ ^ 甲基乙基酮、曱基異丁基酮及環己_)，㈣^甲基㈣胺與二甲亞颯)與水。土、黏^、淋劑或栽劑包括例如：天然礦物磨粉(例如：高嶺合成礦物磨ΙΓ例如白1呂鎮土、蒙脫土與石夕藻土）、之固態載劑包括例如夕酸、馨土及石夕酸鹽）。粒劑大理石、浮石、海、、包H刀碎天_石（例如：方解石、顆粒’有機物質細_如:石：f機或有機磨粉之合成桿）。 1例如·鋸屑、椰子殼、玉米穗與煙草 [例如子性與陰離子性界面活性劑方私-_、續酸燒基§旨、硫酸燒基醋及續酸芳基 •43- 201006383 酯)]，及白蛋白水解產物，等等。勻散劑包括例如：木質素亞硫酸鹽廢液與曱基纖維素。製劑（粉劑、粒劑及乳液）中亦可使用結合劑，其實例包括叛甲基纖維素、天然或合成聚合物（例如：阿拉伯膠、聚 5 乙烯醇與聚乙酸乙烯酯）。亦可使用染色劑’其實例包括：無機色素(例如：氧化鐵，氧化鈦與普魯士藍）、有機染料(如：茜素染料，偶氮染料或金屬酞花青染料），及微量元素如：鐵、猛、硼、銅、始、鉬與鋅之鹽類。 β 10 該調配物中通常包含0.〇1至95重量％抑丹尼，以0.1 至90重量％之間較佳。處理種子時，抑丹尼用量可為每公斤種子使用0.001克至50克’較佳為每公斤種子使用〇〇1克至10克。下文中參考下列實例更詳細說明本發明化合物之製法 15 與用途。然而，本發明不應受此限制。生物試驗與製備實例 ❹ 試驗化合物 3,4-二氯-2’-氰基-1，2-噻唑-5-曱醯替苯胺（俗名：抑丹尼 2〇 (isotianil)) 除草劑(俗名） H-1 :芬殺草(Fen〇Xaprop-P-ethyl) H-2 .炔 α号草酮(oxa(jiargyi) (試驗除草劑製法） 201006383 載劑：丙鲷 =面活性劑：笨甲基氧由上述用量載劑及界 1伤重篁比合，加水稀釋所得製劑與抑丹尼（1份重量比)混種子處理法：取量抑丹尼水懸劑為止。使用攪拌器裹覆預定量之上: u種名稱；Nihonbare)，处抑丹尼水懸劑後，風乾調理。 @ 試驗實例測定降低除草化合物對水稻之植物毒性之效果 10 (方法）取預先經過抑丹尼水懸劑處理之水稻種子播種在已填充水稻田土壌之塑膠盆（100cm2)中，然後於溫室中生長。當植物達2.5葉期時，自上方喷灑預定量之試驗除草劑稀釋液。處理後1天，對植物灌水達3公分深度。處理3週後， 15 決定稻植株出現之植物毒性。分析水稻植株之植物毒性時，藝 100%表示完全死亡，0%表示沒有出現植物毒性效應。其結果綜合說明於下表1。表1 除草活性化合物（g/ha) 1 抑丹尼(isotianil)(g/100Kg 種子） 0 200 ----—««. 400 無〇 _ 0 10 H-l(l〇〇) I 60 20 —-—-. 20 H-2(60) 90 90 60 -45-201006383 e ❿ < Μ Μ 98-014-01p_com 97-342-01p_com a ο s 9 0's cn cn 97-336-0 lp_com 97-287-01p_com ε ο ο ν〇CNJ ι> 97-205-0 lp_com No domain FR notification 1_ transformation result | or strain A5547-127 676, 678, 680 RBMT15-101, SEMT15-02, SEMT15-15 T-120-7 5345 CBH-351 T45 transgenic phenotype glufosinate tolerant Male and glyphosate-tolerant CPB and PVY-resistant glufosinate-tolerant lepidopteran-resistant glufosinate-tolerant and lepidopteran-resistant glufosinate-resistant control project soybean corn potato beet corn Rapeseed institutions (Monsanto) AgrEvo Pioneer Monsanto AgrEvo Monsanto AgrEvo Aike Eve Extension Application Number** * 96-068-01p Application 1_ 98-014-01p 97-342-0 lp 97-339-01p 97-336-01p 97-287-01p _i 97-265-01p 97-205-Olp B-43 B -44 B-45 B-46 _1 B-47 _ί B-48 1 _1 B-49 20 3 8 3 6 < Final EA and judgment ί 1_C 97-204-0 lp-com 97-148-0 lp_com 97- 099-0 Lp_com 97-013-01p_com 97-008_01p-com 96-317-0 lp_com Prepare EA**** or risk assessment FR notification conversion results or strain RBMT21-129 with RBMT21-350 RM3-3 ' RM3-4, RM3-6 GA21 31807 and 31808 strains G94-1 ' G94-19, G-168 MON802 transgenic phenotypes of type CPB and PLRV resistant males glyphosate-tolerant bromoxynil-resistant and lepidopteran resistant Type-change oil-type glyphosate-tolerant and ECB-resistant control project potato, waxy corn, soybean, corn plant (AgrEvo) Monsanto, Mongo, Monsanto, Monsanto, Calgene, Dupont, Du Pont ) Monsanto Extension Application No. *** Application 97-204-0 lp 97-148-Olp 97-099-0 lp 97-013-Olp 97-008-01p 96-317-Olp B-50 B -51 B-52 B-53 B-54 B-55 20 3 8 3 6 < ta 〇S 〇τ-^ 〇<Ν ο B ο υι α, cp 〇〇1 Ό OS 96-068-0 lp -C〇m 96-05 l-01p_com 96-017-01 p-com a O o a1 cp (N Ο 95-338-01p-com preparation EA* (4) or risk assessment FR notification 1_ conversion result or System DBT418 1 strain plus FLAVRSAVR strain t 00 ' t 5 CN — ^ rK CN CO , rf 4 r·^ N 〇〇 inch S 卜b m < N < N ^ ^ < 55-1 > 63- 1 On Ο O i—· 00 00 ο ο CZW-3 V〇^ g έ ^ CQ _ <NH ^ o Bu H , <N 00 ^ ^ Transgenic Gene Phenotypes European Corn Borer Resistant Changes Fruit Ripening Glufosinate-tolerant PRSV-resistant European corn borer-resistant CMV, ZYMV, WMV2-resistant CPB-resistant control project Corn Panzao Soy Papaya Corn Squash Potato | Institution 1_DeKalb Calgene ) AgrEvo Cornell U Monsanto Asgrow Monsanto 1 Extension Application Number *** 92-196-Olp 95-093-01p Application 96 -291-01p 96-248-01p 96-068-0 lp 96-05l-01p 96-017-01p 95-352-01p . 1 _ _1 95-338-01p B-56 B-57 B-58 B- 59 B-60 B-61 _______1 B-62 -6ε· Final EA and judgment 1_ 95-324-0 lp_com 95-256-01p-com 95-228-01p_com 95-195-0 lp_com 95-179-01p one com 95-145-01p—com 95-093-01p_com 95-053-01p_com Silver E A**** or risk assessment FR notification transformation results or strain rn cn 35 IN 19-51a MS3 Btll 2 strain plus FLAVRSAVR strain B16 MON 80100 _1 8338 Transgenic gene phenotype changes fruit mature sulfonylurea tolerance male稔European corn borer resistant type change fruit mature glufosinate-tolerant lepidopteran resistance-type fruit control project N/ny cotton aphid corn maize corn corn plant Agritope DuPont (Du Pont) plant genetics Plant Genetic Systems Northrup King Calgene DeKalb Monsanto Monsanto Extension Application Number *** 92-196-Olp Application 95-324-Olp ... 95-256-0 lp 95-228-01p 95-195-01p 95-179-01p 95-145-01p 95-093-01p 95-053-01p B-63 B-64 B-65 B-66 B- 67 B-68 B-69 B-70 m < 95-045-0 lp a com 95-030-01p a com 94-357-01p a com 94-319-01 p a com 94-308-01 p a Com 94-290-0 lp_com 94-257-0 lp-com pre-EA magnetic* or risk assessment cd <ϋ cp in <N FR notification 10-Mar-1995 conversion knot Or strains 1445, 1698 20 strain plus FLAVRSAVR strain T14, T25 strain 176 _1 531, 757, 1076 f cd Q f BT6, BT10, BT12, BT16, BT17, BT18, BT23 transgenic phenotype mature glyphosate tolerant Change fruit mature glufosinate-tolerant Lepidoptera-resistant lepidopteran-resistant type Reduced fruit polygalacturonase content 鞠 'Hybrid resistance control project cotton WD diso 1 Corn maize cotton ψ Potato plant (Monsanto Monsanto, Calgene, AgrEvo, Ciba Seeds, Monsanto 4 ·§ _ € S « +屮i $ (5)缌g 2 Monsanto Extension Application No. *** 92-196-0lp Application 95-045-01p 95-030-01p 94-357-01p 94-319-0lp 94-308-01p 94-290-0 lp 94-257-01p B-71 B-72 B-73 B-74 B-75 B-76 B-77 < Μ t: 94-230-0 lp_com ( 94-228-01p_com 94-227-0 lp_com 94-090-0 lp-com 93 -258Olp_com 93_196_01p_com 92-204-0 lpcom 92· 196-0 lp—com Preparation or risk assessment 94-228-0 lp_ea 92-204-0 lp_ea _ FR notification 24. Jan 9 5 3-Oct-1994 22. Feb 94 13-Dec-1994 19-Oct-1992 Conversion results or lines 9 strains plus FLAVRSAVR strain 1345-4 strain N73 1436-111 pCGN3828-21 2/86-18 and 23 40-3 -2 BXN ZW-20 FLAVR SAVR Transgenic gene phenotype changes fruit ripening changes fruit ripening changes fruit ripening changes oil type glyphosate-tolerant bromobenzonitrile-resistant WMV2 and ZYMV resistant-type fruit ripening control project. •iftn 4.D .4fm .•i/m MV» Soybean Cotton Pumpkin Plant Calgene DNA Plant Tech Calgene Calgene Monsanto Calgene Upjohn Calgene Extension Application No. *** 92-196-0 lp 92-196-01p Application 94-230-0 lp 94-228-0 lp 94-227-01p 94-090 -0 lp 93-258-Olp 93-196-01p 92-204-0 lp 92-196-0lp B-78 B-79 B-80 B-81 B-82 B-83 B-84 B-85 201006383 According to the present invention, istanic can be formulated into various preparation forms. 41 Clearly, examples thereof include wettable powders, granulated wettable powders, water's valleys, powders, and liquids. Formulation, AL preparations, aqueous suspensions agent and microcapsule preparation. It is also possible to use a coated seed preparation. These preparations can be prepared according to known methods by mixing effluent with a developing agent (eg, a liquid or solid diluent or a manned.) and a surfactant (eg, an emulsifier and/or a leveling agent): When water is used as the developing agent, for example, an organic solvent may be used as a secondary dilution or a carrier including, for example, an aromatic hydrocarbon (10) such as diphenylbenzene or methacrylic acid, or a chlorinated aliphatic hydrocarbon or a chlorinated aliphatic hydrocarbon. Classes (eg, gas benzene, vaporized ethyl methane), aliphatic hydrocarbons [eg cyclohexane, and paraffins (eg, stone, knife)], alcohols (eg: _ class (eg: C-, ¥7 Its PA and secret class, with self-purifying class ^ ^ methyl ethyl ketone, decyl isobutyl ketone and cyclohexyl _), (d) ^ methyl (tetra) amine and dimethyl hydrazine) and water . Soil, viscous, leaching agent or planting agent includes, for example, natural mineral milling (for example: kaolin synthetic mineral honing such as Bai 1 Lu Zhen soil, montmorillonite and Shiyaba soil), and the solid carrier includes, for example, icylic acid , Xintian and Shihua Salt). Granules Marble, pumice, sea, and H-cutting _ stone (for example: calcite, granules 'organic material fine _ such as: stone: f machine or organic mill synthetic rod). 1 For example, sawdust, coconut shell, ear of corn and tobacco [for example, sub- and anionic surfactants - _, continued acid base §, sulfuric acid ketone and carboxylic acid aryl • 43- 201006383 ester)] , and albumin hydrolysate, and the like. The dispersing agent includes, for example, lignin sulfite waste liquid and mercapto cellulose. A binder may also be used in the preparation (powder, granules and emulsion), and examples thereof include betray methyl cellulose, natural or synthetic polymers (e.g., gum arabic, polyvinyl alcohol and polyvinyl acetate). Coloring agents can also be used, examples of which include: inorganic pigments (for example: iron oxide, titanium oxide and Prussian blue), organic dyes (such as alizarin dyes, azo dyes or metal phthalocyanine dyes), and trace elements such as: Iron, sulphur, boron, copper, salt, molybdenum and zinc salts. 10 10 The formulation usually comprises from 0.1 to 95% by weight of dandelion, preferably from 0.1 to 90% by weight. When the seed is treated, the dose of dandan may be from 0.001 to 50 g per kg of seed', preferably from 1 to 10 g per kg of seed. The preparation of the compound of the present invention and its use are explained in more detail below with reference to the following examples. However, the invention should not be limited by this. Biological test and preparation examples ❹ Test compound 3,4-dichloro-2'-cyano-1,2-thiazol-5-indoleaniline (common name: Isotianil) herbicide (common name) H-1 : Fen〇Xaprop-P-ethyl H-2 . acetyl (jiargyi) (test herbicide preparation method) 201006383 Carrier: propyl hydrazine = surfactant: stupid methyl Oxygen is mixed with the above-mentioned dosage carrier and the boundary 1 heavy weight ratio, and the preparation obtained by adding water is mixed with the anti-danny (1 part by weight) mixed seed treatment method: the amount of the denier suspension is taken up. Above the amount: u species name; Nihonbare), after the Danny water suspension, air conditioning. @Test examples to determine the effect of reducing the phytotoxicity of herbicidal compounds on rice 10 (Method) The rice seeds previously treated with the suspension of dansini suspension were sown in plastic pots (100 cm2) filled with rice fields and then in a greenhouse. Growing. When the plant reaches the 2.5 leaf stage, a predetermined amount of the test herbicide dilution is sprayed from above. One day after the treatment, the plants were irrigated to a depth of 3 cm. After 3 weeks of treatment, 15 determines the phytotoxicity of the rice plants. When analyzing the phytotoxicity of rice plants, 100% means complete death and 0% means no phytotoxic effect. The results are summarized in Table 1 below. Table 1 Herbicidal active compound (g/ha) 1 Isotianil (g/100Kg seed) 0 200 -----««. 400 无〇_ 0 10 Hl(l〇〇) I 60 20 —- —-. 20 H-2(60) 90 90 60 -45-

Claims

201006383 VII. Scope of application for patents: 1. A method for reducing phytotoxicity to seed crops by 3,4-dichloro-2'-cyano-1,2-thiazole-5-oxime before sowing Aniline treated seeds. 2. According to the method of claim 1, wherein the seed crop is water 5 rice, and the method reduces plant toxicity to rice seedlings after direct sowing. 3. The method of claim 1, wherein the seed crop is rice, and the method reduces plant toxicity to seedlings during seedling raising in the nursery box. Ίο 4. The method of claim 1, wherein the seed crop is rice, and the method reduces phytotoxicity to rice seedlings after transplanting into the field. 〇 -46- 201006383 IV. Designated representative map: (1) The representative representative of the case is: (No). (2) A brief description of the symbol of the representative figure: No. 5. If there is a chemical formula in this case, please disclose the chemical formula that best shows the characteristics of the invention: None 201006383 J._Patent specification:—:.......: (Do not change the format and order of this manual, please do not fill in the ※ part)

※Application number: Office ※Application date: Public^pc classification: 1. Invention name: (Chinese/English) Method for reducing phytotoxicity METHOD OF REDUCING PHYTOTOXICITY II. Abstract of the invention: The present invention relates to a reduction of seed crops A method of phytotoxicity in which seeds are treated with 3,4-dichloro-2'-cyano-1,2-thiazol-5-nonanilide prior to sowing. III. English invention summary: A method of reducing phytotoxicity to seed crops by treating the seeds with 3,4-dichloro-2'-cyano-l, 2-thiazole-5-carboxanilide before seeding. 201006383 TECHNICAL FIELD The present invention relates to a method of reducing phytotoxicity. It is expressly stated that the present invention relates to a method of treating crop seeds with a particular compound to reduce its phytotoxicity to crops. [Prior Art] 某些 Some types of isothiazolamide (which is disclosed in JP2001522840T) which are known to the public to have phytotoxic activity are described in JP 2 〇〇 4346 〇 3 A). In addition, it is also known that in the nursery box, _ dicyanyl-u ♦ 曱替苯苯苯苯苯苯苯苯JP2007137833A). Dogs (plants close to ^ gradually need to save manpower and low cost of the shaft age. Therefore, more labor, such as: in the nursery box towel preparation for low spring ❹ and 'because of the phytotoxicity of herbicides For the rice planted after transplantation, it is extremely necessary to solve this problem as soon as possible. [Inventors] The present inventors have found that the use of iridone (is〇ti reduces the phytotoxicity caused by herbicide treatment, for example) The invention can also be used for transplanting cultivated crops==cutting crop seeds and then cutting, and also obtaining toxic effects of reducing chemical treatment (for example: herbicides) on plants during seedling raising and after transplanting t to = 201006383 [Embodiment] The herbicidal active compound which can reduce phytotoxicity by the method of the present invention is not particularly limited, but examples thereof include the following compounds: Ethyl CoA carboxylase (ACCase) inhibitor, ^" Oxygen-oxypropionic acid S-type ACCase inhibitors: clodinafop-propargyl, Cyhal〇fop_butyl, diclofop-methyl, fen〇xaprop_p_ethyl Fluazifop-P-butyl, hal〇xyf〇pR-methyl, propaquizafop, qUizai〇fop-p_ethyl and oxazolamide Metamifop); % Axyl-type ACCase inhibitors: alloxydim, butroxydim, dethodim, cycloxydim, profoxydim, chlorpyrifos Grass (sethoxydim), tepraloxydin and tralkoxydim; and base-based AC-type ACCase inhibitor: 嗤琳草g (pinoxaden). Acetyl lactate synthase (ALS) Inhibitor, continued S-type urea-type ALS inhibitor: amid 〇 ur ( (amid 〇 ur ur ( azi azi azi azi azi azi azi azi azi azi azi azi azi azi azi azi ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami ami Chlo〇rsulfuron, cinosulfuron, cyclosulfamuron, albendron 201006383 (ethametsulfuron- Methyl), ethoxysulfuron, flazasulfuron, flupyrsulfuron-methyl-Na, foramsulfuron, halosulfuron-methyl , iazosulfuron, iodosulfuron, mesosulfuron-methyl, metsulfuron-methyl, nicosulfuron, epoxy continuation Oxalsulfuron, primisulfuron-methyl, pyrazosulfuron-ethyl, rimsulfuron, sulfometuron-methyl, sulfosulfuron ), thifensulfuron-methyl, _ triasulfuron, tribenuron-methyl, trifloxysulfuron, triflusulfuron-methyl, trifluoro Tritosulfuron, orthosulfamuron, TH547 and NC620; °11 linonicone (ALS) inhibitor: imazapic, beta rice oxalate 111 & 2311^ 11^>61^-11^1^1), imazamox haze 11^281110\), imazapyr, Imazaquin and imidazepyryr; triazolopyrimidine-type ALS inhibitors: cloransulam-methyl, diclosulam, florasulam, Sulmetsulam, metosulam and penoxsulam; pyrimidine-based salicylate ALS inhibitors: bispyribac-Na, pyrithione (pyribenzoxim) ), pyrifalid, pyrithiobac-Na, pyriminobac-methyl, and pyrimisulfan; and 201006383 bis-sodium ketone-type ALS inhibitor: fluoroketone Flucarbazone-Na, propoxycarbazone-Na and thinencarbazone. Photosynthesis (Photosystem II) inhibitors, three well types: ametryne, atrazine, cyanazine, desmetryne, dimet:hametryne ), prometon, prometryne, propazine, simazine, simetryne, terbumeton, terbuthylazine ), terbutryne and trietazine; triaconone type: hexazinone, metamitron and metribuzin; : amicarbazone; urinary bite type: bromacil, lenacil and terbacil; ketone type: chloridazon; phenyl· · Aminic acid S type: desmedipham and phenmedipham; urea type: chlorobromuron, chlorotoluron, chloroxuron, dimefuron , diuron, ethidimuron, fenuron, fluometuron, isoproturon, Isolon, lisuron, methabenzthiazuron, metobromuron, metaxuron, monolinuron, 201006383 neburon , siduron and tebuthiur〇n; guanamine type: propanil and pentanochlor; nitrile type; bromofenoxim, bromoxynil and Ioxynil; benzopyrene 2 ketone type: bentazon; and phenyl-rough type: pyridate and pyridafol. Through the photoinitiation (photosystem Π) toxicity inducer, the combination of bite rust type: diquat and paraquat. Protoporphyrinogen oxidase (PPO) inhibitor, diphenyl _ type: acifluorfen, bifenox, chlomethoxyfen, fluoroglycofen , fomesafen, halosafen, lactofen, oxyfluorfen and chlomethoxynil; phenyl bite type: isopropyl grass Fluazolate and pyraflufen-ethyl; N-phenylene-imine type: cinid〇n-ethyl, flumioxazin and flumiclorac -pentyl); 0 plug two ° sitting type: fluthiacet-methyl and flu. Thidiazimin; 0 exhalation type oxadiazon and oxadiargyl; triterpenoid type: afafenidin, ethyl gram grass 201006383 (carfentrazone -ethyl) and suifentrazone; slogan: ketone (pent〇xaz〇ne); 'bite-ketone type: benzfendizone (benzfendizone) and fluprofen (butafenacil) And other pyraclonil, profluazol and flufenpyr-ethyl. Carotenoid biosynthesis inhibitor, 1. PDS inhibitor ❹ 善 0 0 ketone type: flufenapyr (norflurazon); ° pyridine carboxamide type: diflufenican (diflufenican) and flufenacet. Picolinafen); and others: beflubutamid, fluridone, flurochloridone and flurtamone; 2. 4-HPPD inhibitor triketone: stone xiaocao _ (mesotrione), sulcotrione, cumin and benzobicyclon and tefuryltrione; iso-salvation: isoxachlortole and iso-alpha _ (isoxaflutole σ ratio saliva: α 草 g g g benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo benzo ;^1〇11); and 201006383 3. Unknown target two zero sitting type: amino triterpenoid (amitrole); different ° ° sitting bite _ type: can be eliminated longitudinal (clomazone); and a base more than type: benzene grass Mystery (aclonifen). EPSP synthetase inhibitor, glycine type: glyphosate and glyphosate-trimesium. Surface amine synthase inhibitor, phosphonic acid type: glufosinate and bialaphos. DHP biosynthesis inhibitor, aminic acid S: subsalt (asulam). Inhibitors of microtubule combination, di- succinil type: bethrodine, butralin, dinitramine, ethalfluralin, oryzalin, sedative (pendimethalin) and trifluralin (trifluralin); amine-based acid-filled vinegar type: amiprophos-methyl (amiprophos-methyl) and sedum (butamiphos); 0 ratio ° stereotype: dithiopyr (dithiopyr) and alpha Thiazolpy; and benzoguanamine type: propyzamide, tebutam, and chlorthal-dimethyl; 201006383 inhibitor of mitosis/microtubule organization, Acetone vinegar type. Champer prp (pl〇rpr〇pham), aniline (pr〇pham) and carbetamide. Inhibitor of very long-chain fatty acid biosynthesis, chlorhexidine type. Acetochlor (Acet〇chi〇r), Aiachl〇r, butachlor, dimethachl〇r ), dimethanamid, metazachlor, metolachlor, pethoxamid, pretiiachlor, propachlor, propisochlor (pr 〇pisoch!or) and thenylamine; diamined, diphenamid, napropamide and naproanilide; oxyhydrobylamine·fluazisamine (flufenacet) and mefenacet; tetrazolinone type: fentrazamide; and others: anilofos, cafenstrole, and piperophos ° cellulose Inhibitors of biosynthesis, nitrile type: dichlobenil and chlorthiamid; benzoquinone type: isoxaben; triterpenoid: fluamine η (flupoxam); and mouth quiz and tick-type: quinclorac. 201006383 Decoupling agent, dinitrophenol type: DNOC, dinoseb and dinoterb. Fatty acid elongation inhibitor (not inhibited ACCase), thioaminate type: butylate, CyCi〇ate, dimepiperate, EPTC, esprocarb, 〇达❹❹ (m〇Hnate), orbencarb, pebulate, prosulfocarb, benthiocarb, pyributycarb, tiocarbazil , triaiiate and vern〇late dithio acid vinegar type: bensulide benzo D-type: grass yellow (benfuresate) and ethoxylated yellow (ethofumesate) and Gas-carbonic acid type: TCA, dalapon and tetrapion ° ❹ auxin herbicide, phenoxy-rebel type: clomeprop, 2,4-D , 2,4-DB, dichlorprop, MCPA, MCPB and MCPP; benzoic acid type: chloramben, dicamba and 2,3,6-TBA ; ° Fortypic acid type: clopyralid, fluroxypyr, picloram, triclopy, quinclorac and quinmerac and 2 01006383 Other: Benzolin-ethyl. An auxin transport inhibitor, o-benzamine type: naptalam; and anilino urea type: diflufenzopyr-Na. Others (unknown mode of action), arylaminopropionic acid type · flamprop-M-methyl and fluprop-isopropyl; ** than 0 sitting Type: difenzoquat; organic arsenic type: DSMA and MSMA; and others: bromobutide, chloroflurenol, cinmethylin, cumyluron, mai Dazomet, daimuron, methyl-dimuron, etobenzanid, fosamine, indanofan, metadan ), ° oxaziclomefone, oleic acid, citric acid and pyributicarb. ❹ These herbicidal compounds (described by common name or derivative name) are mainly described, for example, in “The Pesticide Manual”, 14th edition (British Crop Protection Council, 2007), or Know the person. The selective herbicidal effect can be achieved in the weeds of crops according to the method of the invention. As used herein, the broad definition of weed refers to any plant that grows on a point where it is not expected to grow. For example, the weeds and crops described below can be used as the subject of the method of the invention -12·201006383. Dicotyledonous weeds: white mustard (sinapiS), amaranth (c叩 this, Leipichum, Gcdium, StenaHa, Chenopodmm, mantle (K〇chia) ), Urtica, pweczo, cockroach, purslane (p(10)-called, Xanthwm, Ip〇m〇ea, p〇lyg〇num) , Weeds W, Yushan, Ghosts, and so on), Solanum, R〇Hppa, Lamium, Turtle Daiura), pansy (ri〇!a), 丨犯 (Ga丨eopsis), poppy (papaver), yarrow (Centaurea), millet chrysanthemum, zhizhicao^, 陌上菜❿·" (4) , Sesbarua, Trjf〇iium, Abuti I〇fj, Lamium, Matricaria, Artemisia, Sesbania, Year of the Year (Sesbania) Pharbitis), dicotyledon: cotton, soybean, beet, 7 cents, carrot, kidney bean (Thaseolus, cowpea), solanum, Linum, ipom〇ea ), wild peony (yicia), smoke (Wz'coiz'awa), Fanyou [々j;co corpse, peanuts [々如办(4), Brassica, high t (Lactuca), Cucumis, Cucurbita)早子叶杂卓·转ΟΕΆ/ζίοοΛ/οα),赛草(/SWflrz'a), 稷(八?《化1/历), 马唐(£)如加哈),梯草草(Phleum), Teal (ροα), grazing grass CFeWwca), snail grass (£7eMiy/we), toxic wheat (/^//^), brome (•firowws), hot wheat (Jvewflt), sedge ( Q; corpse erw·?), sorghum ("Sorg/mm", small-13-201006383 麦草,雨久花(Μί>(10)c/zorza), mb草草 (Fimbristyslis), 龟姑(Sagittaria), .% 芋( Eleocharis), Μ草(•SWr/jM·?), 雀转(P〇s/?a/ww), platyrhynchos, sugar-stable 〇4#like), see Mai Niang as/叩(10)), Bermuda grass, Commelina, Brachiaria, Leptochloa, etc. Monocotyledon: rice (Or_yzW, maize (ZeaJ, wheat (Trz7z_cww), barley (Hordeum), Qin's (Avena), I (Secale), sorghum (Sorghum), nine (Panicum), Gan, ( Saccharum), color game, (Musa), Ananas, Asparagus, l (Allium), special. In addition, the method of the present invention is not limited to the above plants, and it can also be used in a similar manner. In addition to other related plants. Further, according to the invention, the method can be used to control weeds in perennial crops, and can be applied to, for example, forests, ornamental afforestation, orchards, vineyards, citrus forests, nuclear orchards, banana gardens, coffee gardens. , tea gardens, rubber gardens, oil palm gardens, cocoa gardens, soft orchards, and beer gardens, etc. It can also be used to selectively control weeds in annual crops. © The method of the invention can be provided between rice and rice weeds Selective control of herbicidal effects. Therefore, the examples of weeds in rice fields that can be controlled are as follows: The following monocots: edulis, broccoli, sedge (7) stealing (four), grosgrain (ZzWmi (4), sinensis (8) (four), it Eye grass (Dopatr Ium), Eclipta, JE: Iaiine, G8 (GrimWaj, Moshang (6), such as m'W, water clove, water celery, buttercup, zea peony (7) ez • (10) you , etc. 201006383 The following dicots: Echinochloa, 稷CPimfcwm), Teeth (10), sedge (QyperM·?), Yujiu, Fimbristyslis, Sagittaria , ·% word (JEleocharis), Scirpus, Alisma, Aneilema, Blyxa, Drumstick grass (five-door caw/owj, eye dish, para Brachiaria, Leptochl〇a, Sphenoclea, and more. More specifically, the method of the present invention can be applied to, for example, the following typical rice weeds. Plant name Dicotyledon: Tianzi Grass water sedge cusp flower sauerkraut indian festival 茚草陌上菜山苋菜母草鳢内长果母草毛叶肾蕨毛蓼

Latin name Marisilea quadrifolia Fimbristylis miliacea Sphenoclea zeylanica Ammannia sp. Rotala indica Koehne Lindernia procumbens Philcox Amaranthus viridis Lindernia dubia L. Penn. Eclipta pro strata Lindernia angustifolia Ludwigia prostrata Roxburgh Polygonum sp. -15- 201006383 Tian Jing Sesbania exaltata Potamogeton distinctus A Benn 洼洼 Ipomoea lacunosa Long-leaved latitude Elaine triandra Schk Java water celery Oenanthe javanica Monocotyledons: Echinochloa oryzicola Vasing Echinochlor colonum E. crus-galli E. sinensis Ischaemum rugosum Alfalfa Panicum sp. Eleusine indica Digitaria sp. Paspalum distichum Broccoli Cyperus iria Aconite C. rotundus Cattle Eleocharis acicularis L. Eleochris kuroguwai Ohwi Cyperus difformis L. Cyperus serotinus Rottboel Water Scirpus mucronatus S. garlic, S. planiculmis, S. sinensis, Scirpus juncoides Roxburgh -16-20100 6383 Aquila sylvestris sylvestris sylvestris sylvestris sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis sinensis Aquatica Sagittaria trifolia Monochoria korsakowii Brachiaria plantaginea Leptochloa chinensis The method of the invention is not limited to the above weeds. It can also be applied to other types of weeds. When the method of the present invention is carried out, the seed of the seedling or the seedling state is not treated with the composition containing the dandy in the seedling box, the paddy field or the hilly land. Specifically, the crop seeds can be treated by soaking or dust coating using the composition. As a result of the treatment, the phytotoxicity of the crops in the nursery box, after transplanting to the paddy field, directly after sowing into the paddy field or on the hilly land is reduced. The treatment according to the invention can be used to treat genetically modified organisms (GMOs), such as plants or seeds. Genetically modified plants (or genetically transformed plants) have been stably integrated into the genome for heterologous genes in plants. The term "heterologous, gene" basically refers to introducing a gene provided or a gene composed outside the plant into the nuclear genome, chlorophyll genome or mitochondrial genome of the gene transfer plant, by expressing the desired protein or polypeptide, Or down-regulate or silently treat another species or other genes in the plant Π 201006383 (eg, using antisense technology, co-repression technology, or RNAi technology [RNA interference]) to confer new or improved agronomic or other properties. A heterologous gene that is present in the genome is also known as a transgenic gene. A specific location in which the & genomic gene is defined in the plant genome is referred to as transformation or gene transfer. Depending on the plant line or plant cultivar, its location and its growth conditions (soil, climate, growing season, nutrition), the treatment according to the invention may also result in superadditive ("potentiating") efficacy. Thus, for example, the following may be expected to exceed the actual expected effect: lowering the rate of application and/or expanding the range of activity and/or increasing the activity of the active compound and composition according to the invention, improving plant growth, increasing high temperature or low temperature Tolerance, improve tolerance to drought or water or soil salinity, increase flowering rate, simplify harvest, accelerate maturity, increase yield, increase fruit, increase plant height, deepen leaf green, early flowering, remodeling The quality of the harvested product and / or increase the nutritional value, increase the sugar content of the fruit, and change the storage capacity and / or processability of the harvested product. Plants and plant cultivars that are more suitable for treatment in accordance with the present invention include those plants (whether obtained through breeding and/or biotechnological means) having genetic material that affects the particular wealth and fitness of such plants. Plants and plant cultivars which are also treated according to the invention are plants which are resistant to one or more biological stresses, ie the plants exhibit better protection against microbial diseases, such as against pathogenicity of nematodes and insect plants. True g, (four), virus and / or viroid. Plants and plant cultivars treated according to the invention are also suitable for plants which are resistant to one or more abiotic stress factors. Abiotic pressure = 201006383 Conditions may include, for example, drought, exposure to low temperatures, exposure to high temperatures, osmotic pressure, flooding, increased soil salinity, increased mineral concentration, exposure to ozone, exposure to glare, nitrogen nutrient availability Limited, phosphorus nutrient availability is limited, to avoid shade. Plants and plant cultivars which may also be treated according to the invention are plants which are characterized by enhanced harvest characteristics. Methods for improving the plant harvest can be, for example, improving plant physiology, growth and development, such as: water use efficiency, water retention

, rate, improve nitrogen use efficiency, enhance carbon assimilation, improve photosynthesis, improve germination efficiency and accelerate maturity. The harvest can be further improved by improving the plant structure (under pressure and under pressure), including (but not limited to): early flowering, flowering control to produce hybrid seeds, seedling growth, plant size, internodes, amount And distance, root growth, seed size, fruit size, pod size, number of cool or spikes, number of seeds per pod or per ear, seed quality, enhanced plumpness, reduced seed scatter, reduced fruit cracking and Lodging resistance. 1. Harvest characteristics include seed formation, such as: carbohydrate content, protein content and composition, nutritional value, reduction of undesirable nutrient compounds, improved processing and improved storage stability. Changli is the resistance that has demonstrated heterosis or hybrid acquired stressors. The typical method for the preparation of such plants is from the male parent (female) (four) (also) hybrid. Hybrid seed blood (4) The head is made by the hybrid I. Parents: Plants sometimes (for example, corn) can be mechanically removed by males (or male flowers), but more often as 201006383 for plant genomes. The genetic determinant in the middle caused the male instability. At this time and especially when it is desired to harvest seeds from the hybrid plants as the desired product, it is generally suitable to ensure that the hybrid plants are fully restored to male stability. It is practiced to ensure that the hybrid plant that contains the genetic determinant responsible for male sex is restored to male genitality. The male gene determinant can be located in the cytoplasm. Examples of cytoplasmic and male convulsions (CMS) are described, for example, in the crucifer species (Brassica genus). However, the male gene determinant can also be located in the nuclear genome. Male plants can also be obtained using plant biotechnology methods such as genetic engineering. A particularly suitable method for obtaining a male plant is described in WO 89/10396, wherein, for example, a ribonuclease such as: barnase is selectively expressed in the cell membrane of the stamen. Inhibition can be restored by ribonuclease inhibitors such as barstar in the expression of cystic cells. The plant or plant cultivar which can be treated according to the invention (which can be obtained by means of plant biotechnology, such as genetic engineering) can be a herbicide-tolerant plant, i.e. a plant which can tolerate one or more specific herbicides. Such plants can be obtained by genetic transformation or selection of a plant containing a mutation that confers tolerance to these herbicides. The herbicide-reducing plant is, for example, a glyph〇sate-tolerant plant, that is, a plant which can tolerate a glyphosate herbicide or a salt thereof. Plants can be transdermally, and many plants that are resistant to silky tolerance can be transformed by transforming plants with the gene encoding the enzyme 5_diolpropionic acid _3_phosphoric acid synthase (EPS^S). . Examples of such Epsps genes are Salmonella (10) r/w... bacteria Αγ〇α gene (mutant strain • 20-201006383 CT7), Agrobacterium (such as ^(10) India) bacterial CP4 gene, encoding petunia EPSPS, tomato EPSPS or cattle The gene for the EPSPS (WO 2001/66704). It can also be a mutated EPSPS. Glyphosate-tolerant plants can also be produced by genetically encoding glyphosate oxidoreductase enzymes. Glyphosate-tolerant plants can also be produced by the expression of a gene encoding a glyphosate acetyltransferase enzyme. Glyphosate-tolerant plants can also be obtained by selecting plants containing natural mutations of the above genes. ❹ G Other herbicide-resistant plants are, for example, herbicides that can inhibit the enzymes, such as: biaphph〇s, phosphinotricin or gluf. 〇sinate) plant. These plant preparation methods are prepared by exhibiting an enzyme which removes the toxicity of the herbicide or a mutant face amidoxime synthase enzyme which can resist the inhibition. One of the detoxifying enzymes is an enzyme encoding glufosinate acetamyl converting enzyme (e.g., bar or (10) protein from Streptomyces species). Also indicated are plants exhibiting glufosinate acetamyl converting enzyme. Nets and other herbicides are also plants that can tolerate herbicides that inhibit the enzymes of the base. The base phenyl group "two = converted to catalytic = base stupid" Black uric acid iJppn. Plants that are resistant to X inhibitors can be improved by transforming plants with a gene encoding a naturally-resistant HPPD enzyme or a HppD enzyme encoding a mutation by the gene of __,: yeast =:= -21- 201006383 acid dehydrogenase enzyme. Other herbicidal touches _ material (four) & = s) inhibition of the secret. Known ALS. (4) Society Reading Lai, Sanyin, Qiansi (5) Benzoic Acid Amine Triterpenoid_ Herbicides. Know the different money of ALS yeast (also known as (four) acid complex, AHAS) =

= 2 = tolerance to 3 doses. It has been documented that the base brain is a sex-tolerant plant. Other materials are also listed in the 6 categories. Other heterozygous _ _ ° ° _ literate plants are also described in, for example, W0 2007/024782. Other methods of tolerance to imipenyl and/or lysine urea planting are mutagenesis, selection or mutation breeding in cell cultures containing herbicides, as illustrated, for example, in soybeans, rice, sugar beet, lettuce or sunflower.

Plants or plant cultivars (produced by plant biotechnology methods, such as those obtained by the genetic defense method) which can also be treated according to the invention are insect resistance gene transgenic plants, that is, resistant to some target insects. plant. Such plants can be obtained by gene transformation or by selecting a spigot containing such insect resistance. As used herein, "insect-resistance gene transfer plants', including any plant comprising at least one transgenic gene, comprising sequences encoding the following: 1) insecticidal crystals from the sacred cloud Proteins or insecticidal fractions thereof, as listed in Crickmore et al., Microbiology and Molecular Biology Reviews (1998), 62, 807-813, updated by Crickmore et al. (2005) Bacillus thuringiensis toxin -22-201006383 Connect to: http: //www.lifesci.Sussex.ac.uk/Home/Neil_Crickmore/Bt/) insecticidal crystalline protein' or its insecticidal part, eg Cry protein CrylAb, CrylAc, CrylF, Cry2Ab , Cry3Ae or Cry3Bb or a protein thereof; 2) a crystalline protein from Bacillus thuringiensis or a part thereof, which is present in the presence of a second other crystalline protein from Bacillus thuringiensis or a part thereof Insecticidal, such as: a binary toxin consisting of Cry34 and Cry35 crystalline proteins; or 3) containing different insecticidal crystalline proteins from Bacillus thuringiensis a hybrid insecticidal protein, such as: a hybrid protein of the above 1) or a hybrid protein of the above 2), for example, a CrylA.105 protein produced by the maize variety MON98034; or 4) any of the above proteins 1) to 3) Some of them (specifically, up to 10) amino acids have been replaced by another amino acid, which can produce higher insecticidal activity against target insect species and/or can expand the affected target insect species. Range and/or introduction of changes into the encoded DNA during selection or transformation, such as: Cry3Bbl protein of maize variety MON863 or MON88017 or Cry3A protein of maize variety MIR604; or 5) Bacillus thuringiensis or Bacillus licheniformis (The insect killing protein, or its insecticidal part, such as the growth of insecticidal (VIP) protein, listed at: http://www.lifesci.sussex.ac.uk/Home/Neil_Crickmore /Bt/vip. html, for example: protein from VIP3Aa protein; or -23· 201006383 6) 7) 8) Secreted protein from Bacillus thuringiensis or Bacillus cereus, which is from Bacillus thuringiensis or An insecticidal activity in the presence of a second secreted protein of the bacterium, such as a binary toxin composed of VIP1A and protein; or a heterozygous killing of a different secreted protein from Bacillus thuringiensis or Bacillus cereus An insect protein such as: a hybrid protein as described above or a hybrid protein of the above 2); or any of the above 1) to 3), some of which (specifically, to (7)) amino acids have been Alkaline acid substitution, which can be used to target insect insects to further insecticidal activity and/or to expand the range of affected stems and/or to introduce changes into the coding during selection or transformation (while still Encodes insecticidal proteins such as: A COT102 VlP3Aa protein. In this paper, the insect resistance gene transgenic plants are used as the combination of the gene combinations of any of the 8 types: = the transgene of the protein, when different '8 stems of the genus are used, the dans can be metamorphosed for different It is indicated that different protein pairs are used: the target insect species range, or insecticidal by non-Hi species, but has a mode of action, such as: unlike insects t, delays insect resistance to plant development. And the combination of the activity of the δ, and the technical methods of the plant or the material which can also be treated according to the invention, such as: genetic engineering method for cultivating the cultivar (produced by plants ~ can tolerate abiotic stresses • 24-201006383 2 such plants You can use the domain to turn to the missing or select the secret of the inclusion mutation (4). _ The pressure tolerance is purely anti-匕d to reduce the poly (octa-ribose) aggregated pARp) gene in b. cell or plant towel performance and / or active transgenic plants. Kit 3. A plant which potentiates the expression and/or activity of a pARG-encoding gene of a plant or plant cell to enhance stress-tolerant transgenic genes. Contains the plant enzymes (including the secretase, the nucleus-acid-converting enzyme, the acid mononuclear thief gland-based invertase, the secret amine gland) encoded by the chitosan adenine dinuclear acid recovery biosynthetic pathway A plant that enhances the stress-tolerant transgenic gene of dinuclear acid synthase or nicotinamide ribose phosphate convertase. Unrestricted examples of plants having the above characteristics are shown in Table A. ^ A ' \ Α-1 The goal or performance of the original crop phenotype / on the response ~~~~~~~~~ acetate lactate synthase (als) ------- scutellaria Class, taste, saliva, and stagnation.密定, Λ «" Ν ~--- pyrimidinyl oxybenzoate, benzoquinone Α-2 B S & base CoA aptaming enzyme aryl oxyphenoxy oleic acid, cyclohexane Ketones, 3 (ACCase), phenyl propyl g, and acid g, dioxin 0, such as isoxaflutol (HPPD) or isoxachlortole ), triketones, such as: mesotrione or sulcotrione, glufosinate, phosphinotricin, A-5 〇-methyl-transformase Lignin content -25 - 201006383 ------ ------- Ύ No· The principle of target or performance - Crop phenotype / Tolerance Α-6 glutamine amine synthase -- ------ Glufosinate, Bialaphos Α-7 Adenylate succinate lyase (ADSL) IMP and AMP synthesis inhibitor Α-8 ----J - --- Adenosyl succinate combined with adenosine succinic acid synthesis inhibitor Α-9 ----- a "U a * ~ * human 1/3, sweet years o-aminophenyl hydrazine Acid synthase tryptophan synthesis and metabolism inhibitor Α-10 nitril hydrolysis Enzyme 3,5-dihalo-4-perylene-phenyl cyanide, such as: Bromoxynil and Essini (ΑXinyi) Α-11 5-dipropoxide propyl ruthenium _3_filled Shikimate oxalate synthase (EPSPS) Glyphosate or sulfosate Α-12 Glycyrrhizin oxidoreductase glyphosate or sulfosate Α-13 protoporphyrinogen oxidase (PROTOX) A basic riddle, cyclic imine, phenyl, υ 类, 0 口衍生物 derivative, phenopylate, 二 2, sitting, etc. Α-14 cells Pigment P450, for example: P450 SU1 xenobiotics and herbicides, such as: sulfonylureas Α-15 Dimboa biosynthesis (Bxl gene) corn motility bacteria (//e/continental turcicum), corn leaf field maydis), Diplodia, European corn culture (10) (4), Lepidoptera, Α-16 CMIII (small alkaline corn seed peptide), plant pathogens such as: suspected bacteria, alternaria, spectroscopy Sclerotina) Α-17 corn-SAFP (zeamatin) plant pathogens such as: suspected bacteria, altemaria, sclerotina, sclerotium «(4), Chaetomium, phycomyces -26- 201006383 No. ---- Principle of achievement of goals or performance Crop phenotype/tolerance A-18 Hml gene Cochliobulus A-19 A-20 Chitinase Glucanase Plant pathogens Plant pathogens A-21 Envelope protein viruses, such as: corn dwarf mosaic virus, jade gas - dwarf virus (com chlorotic dwarf νϊηις) A-22 Suyun gold spore Bacillus (4) Shenyan (10) Lepidoptera, Coleoptera, Diptera 'C. elegans, for example: thuringiensis) Meal, iqp corn moth (0 price; > 2 as secret 2/&), corn ear night 3, cactus Bacillus (5acz_Moth zea), armyworm, for example: grass sticky cere called) toxin, luminescent bacterium M^Spodoptera frugiperda), corn root gas, (Photombdus) and the genus Mycobacterium sylvestris (/Sfesawk), small To the tiger, the eagle {Xenorhabdus j -3⁄4. -ψ--. corn borer, weevil A-23 3-hydroxysteroid oxidase Lepidoptera, Coleoptera, Diptera, nematodes, for example: corn moth (QsinVz/a s·), Heliothis zea, sticky insects, for example: A[Spodoptera frugiperda), corn root blast, worm main moth OSesaww·?·), terrapin, Asian-maize, weevil A-24 peroxidase' ~--- Lepidoptera, Coleoptera, Diptera, C. elegans, for example: Maize moth, also zeaj, Zygomycetes, for example: Spodoptera frugiperda, corn rootworm, Plutella xylostella, small Earth tiger, Asian corn borer, weevil A-25 aminopeptidase inhibitor, for example: Lepidoptera, Coleoptera, Diptera, Nematodes, for example: White Moon female acid fe-based enzyme inhibitor corn-filled moth (OsirWa m/Ma/i's), corn steady night (LAPI) moth (//e/z'oite zea), armyworm, for example: grass sticky - win (Spodoptera frugiperda), corn root gas, -27- 201006383 No A-26 A-27 A-28 ______ Theory, ~ 曰孙 or performance of the original crop phenotype / tolerance ... 夕卜? φ~~~~-- ~ " 11 worm main stem night Moss (Sesamia sp.), small tiger, Asian corn borer, weevil corn rootworm 1 which is a hard collectin '~~~--___ Lepidoptera, Coleoptera, Diptera, gas meter, extinguished · . Rice-filled moth (C^strzWa m^7a/z's), snails, snails, snails, snails, snails, snails, snails, snails, snails, snails, snails, snails, snails, snails, snails Earth tiger, Asian corn fill, weevil protease inhibitor, for example: cysteine protease inhibitor, storage protein inhibitor (patatin) 'thiol protease inhibitor (virgiferin), rim weevil, corn root Insect A-29 ribosomes deactivated proteins Lepidoptera, Coleoptera, Diptera, Nematodes, for example: Corn moth (C&irWa, Spodoptera frugiperda (//Άοί/ώ zeaj, armyworm, for example: grass sticky) XSpodoptera frugiperda), corn root blast, worm main moth, small tiger, Asian corn borer, weevil A-30 corn 5C9-polypeptide lepidoptera, coleoptera, diptera, nematode, eg: corn fill Moth ((^inWa, corn zea), armyworm, for example: grass worm + Spodoptera frug^erch), corn root genus, worm main moth (5^<3/<3 flat· , Little Tiger, Asian Corn Fill, Weevil 201006383 I~S_ —— No. Achieved goal or table Principles Crop phenotype/tolerance ~ A-31 ----_____ tlMG-CoA reduction|| — Lepidoptera, Lepidoptera, Diptera, Nematodes, eg: Corn-filled moth (3⁄43⁄4'mV? m /Mfl / sentence, corn ear 峨峨 fHe / ζ 'οί / ζώ zeaj, sticky insects, for example: grass sticky A (Sp〇doptera frugiperda), corn root meaning, surname Spodoptera (S& aw/aj: /? ·", small tiger, Asian ΛΑ ^--- inhibit protein 螟, weevil chloroacetate aniline, such as: Alachlor, Acetochlor, A-33 ~^ί _Dimethenamid _2,4-D, Mecoprop-P technology U-treated plant or plant cultivar (plant yield, quality and quality? The engineering product produces a modified product that exhibits improved properties, such as / sub-stability and / or changes in the specific components of the harvested product. 1) 2) Transgenic plants of the cell, compared to the fine powder of the wild plant In terms of physicochemical properties, in particular, the proportion of straight amylose/amylopectin in the linear chain, the degree of branching, the average/or the lake, the gel reading, the size of the secret granules, and the 乂心寺 have all changed, so it is more suitable for specific use. A modified non-starch carbohydrate or a synthetic non-starch carbohydrate having a modified nature compared to a non-genetic plant: a genetically transgenic plant. Examples thereof are plants producing polyfructose, inducible inulin and fructan type, plants producing α-1,4 glucan, -29-201006383 producing α-1,6 branches α-1,4 A plant of sugar, a plant that produces alternan. 3) Geneogenic plants that produce hyaluronan. A genetically modified plant that is particularly suitable for treatment according to the present invention is a plant comprising a transformation result or a combination comprising a transformation result, which is in the United States to the United States Department of Agriculture (USDA) Animal and Plant Health Inspection Service ( The Animal and Plant Health Inspection Service (APHIS) applies for the subject of a non-regulated status, whether or not such application is approved or still under review. This information can be obtained from APHIS (4700 River Road Riverdale, MD 20737, USA) at any time, for example, from the Internet (URL http://www.aphis.usda.gov/brs/not_reg.html). In the case of this application, the applications for such non-regulated status that are still under APHIS audit or approved by APHIS are listed in Table B and contain the following information: Application: Application Slot Number. The technical description of the conversion results can be found in the respective application documents obtained from APHIS, for example: ApHIS website, refer to this application number. These descriptions are incorporated herein by reference. Extended application: refers to the previous application that requires extension.机构 Agency · Name of the institution submitting the application. Control project: related plant species. Transgenic gene phenotype: the nature of the plant as a result of the transformation. Conversion result or line: The name of the conversion result (sometimes called a line) that requires an unregulated status. Aras literature: The various publications from APHIS and the towel (4) are available under the APHIS request. • 30- 201006383 Final EA and Judgment Preparation EA**** or Risk Assessment 06-271-01pjpea FR Notice 5-Oct-2007 !- Conversion Results | or Line MIR-162 Maize IFD-19890-1 and IFD-19907- 9 HT-98140 COT67B DP-305423-1 GHB614 MON 89034 356043 Transgenic gene phenotype change flower color glyphosate and ° meter 坐坐 g similar tolerance lepidopteran resistant high oleic acid glyphosate tolerant Europe Corn gluten-resistant glyphosate and acetaminolate control | Project νή Carnation Corn Cotton Soybean Cotton Corn Soybean New Synta (Florigene) Pioneer Pigenter Syngenta Pioneer Bayer Bayer CropScience Monsanto Pioneer Extension Application No. *** Application 07-253-01p 07-180-01p 07-152-0 lp 07-108-01p 06-354-01p 06 -332-01p 06-298-Olp 06-27 l-01p r—( <N B-3 B-4 B-5 B-6 B-7 B-8 201006383

Final ΕΑ and judgment 04· 110-01 p-com preparation ΕΑ or risk assessment 04-110-01p_pea ce # Sr φ Is 7 us铼FR notification 8 SSS rq ο ο oj* (N (N (N ^ ^ ^><=> ^ Bu CO inch r4 (SO CN range and status; 12-0ct-2005; 11.04.2005; 18.11.2004; 24.09.2004; 05.01.2004 Conversion result or strain 3272 XI7-2 J101, J163 ASR368 transgenic lean phenotype ester synthase tolerant heat-stable α-amylase papaya circovirus-resistant glyphosate-tolerant glyphosate-tolerant control project corn papaya town sorghum New Jersey (Syngenta) University of Florida-3 | ^ SI Monsanto and Scotts Extension Application No. μ* Application 05-280-01p 04-337-Olp 04-110-Olp 03-104 -01p B-9 B-10 B-ll B-12 οά — 20 3 8 3 6 ο ο Final ΕΑ and judgment 06-234-01p_com 06_178-01p a com B oo <N CO 04-264-0 lp_com 04 -229-0 lp-com 04-125-01p_com 04-086-0 lp_com 03-353-01p_com 1 Preparation **** or risk assessment 06-234-01p_pea 06-178-0 lpjpea 04-362 -0 lp_pea 04-264-0 lp_pea 04-229-0 lpjpea 04-125-01p_pea 04-086-01p_pea 03-353-01p_pea FR Notice 4-Dec-2006 ; 08.09.2006 02.08.2007 ; 05.02.2007 23- Mar-2007 1 22.02.2007 ; 10.01.2007 13-July-2007 1 16-May-2006 03.02.2006 ; 27.09.2005 06.01.2006 ; 12.08.2005 03.01.2005 ; 24.11.2004 ; 4-Oct-2004 07.08 .2005 ; Transformation results or strains LLRICE601 MON 89788 MIR604 LY038 88017 MON 88913 59122 Transgenic phenotype glufosinate-tolerant glyphosate-tolerant anti-corn rootworm high-alkaline corn-root-resistant glyphosate Tolerant Corn Rootworm Tea on Beam Soybean ή 屮棉花棉花 1 1 Bayer CropScience Monsanto Syngenta ARS Monsanto Monsanto Monsanto Road Dow Extension Application No. *** 98-329-01p Application 06-234-01p 06-178-01p 04-362-01p 04-264-0 lp 04-229-0 lp 04-125-Olp 04-086-0 lp 03-353-01p B-13 B-14 B-15 B-16 B-17 B-18 B-19 B-20 -ee丨 Final EA and Judgment 03_323-01p—com 03-181-01p_com 03-155-01p_com 03_036_01p—com 03-036-02p_com 02-042-0 lp_com Ol-324-Olp-com Preparing EAAAifcA or risk assessment 03-323-Olpjpea 03-181,01p_pea 03-155- 01p_pea 03,036-01p__pea 03-036-02p_pea FR Notice 01.07.2005 17-Mar-2005;19-0ct-2004 01.11.2004; 17.08.2004 20.07.2005 ; 28.01.2005 13.08.2004 ; 9-Mar-2004 1 Os 〇〇<NO 2 s transformation result or strain H7-1 TC-6275 COT 102 281-24-236 3006-210-23 LLCotton25 RT200 transgenic phenotype-resistant glyphosate-tolerant lepidopteran resistant Glufosinate-tolerant lepidopteran-resistant lepidopteran-resistant lepidopteran-resistant grass-filled tolerant glyphosate-tolerant control project sugar beet corn cotton cotton cotton cotton rapeseed plant 1_ Monsanto Monsanto) Dow New Syngenta Mycogen / Dow Mycogen / Dow Aventis Monsanto Extended Application Number *** 00-136-01p 98-216-Olp Application 03-323-01p 03-181-01p 03-155-01p 03-036 -01p 03-036-02p 02-042-0 lp 01-324-01p B-21 B-22 B-23 B-24 B-25 B-26 B-27 201006383

1 Final EA and judgment S 〇〇 (N a O on! CN \D 〇<N o 01-137-01p-C〇m , 1 01-121,01p-com B 〇cp will cn ο -1 00- 136-0 lp—com 1 00-011-0 lp_com j 99-173-01p a com 1 -1 Prepared EA**** or Risk Assessment! FR Notification Conversion Results or Lines MSI and RF1/RF2 Topas 19/2 MON 863 Vector 21-41 cotton line 15985 line 1507 NK603 RBMT22-82 transgenic phenotype glufosinate-tolerant and controlled pollination glufosinate-tolerant maize rootworm resistant type reduced nicotine lepidopteran resistant lepidopteran Sex-type glufosinate-tolerant glyphosate-tolerant PLRV and CPB-resistant control project rapeseed rapeseed corn poetry cotton corn maize potato institution Aventis Aventis Monsanto (Aventis) Monsanto) Vector Monsanto Mycogen c/o Dow and Pioneer Monsanto Monsanto Extension No. *** 98-278-01p 1 97-205-0 lp 97-099-0 lp 97-204-0 lp Application 01-206-0lp 01-206-02p Ol-137-Olp Ol-121-Olp 00-342- 0 lp 00-136-01p 00-011-01p 99-173-0lp B-28 B-29 B-30 B-31 B-32 B-33 B-34 B-35 丨-丨 201006383

The final EA and the judgment 98-349-0 lp-com _1 s 〇o we m cn 00 On a ο ο ο os <Ν cn 00 a ο ο ΟΟ (Ν ύ . 1 98-238-01p_com —1 ! B ο ο ο 七 CN 00 ΟΝ 98-173-01p_com 1 Preparation or Risk Assessment FR Notification Transformation Results or Line MS6 CDC Trident (Triffid) LLRICE06 > LLRICE62 MS8 and RF3 GU262 RT73 GTSB77 ί Transgenic Gene Phenotype Glufosinate Tolerance Type and ambitious 醯醯 urea urea herbicide glufosinate-tolerant glufosinate-tolerant and controlled pollination glufosinate-tolerant glyphosate-tolerant glyphosate-tolerant Control project linseed rapeseed soy plant Agra Evo ^ a 1 Μ ^ AgrEvo AgrEvo AgrEvo Monsanto M w -3 I Ί ^ ^3 Extended Application No. *** 95-228-Olp Application 98-349-01p 98-335-Olp 98-329-0 lp 98-278-0 lp 98-238-01p 98-216-0 Lp 98-173-01ρ B-36 B-37 B-38 B-39 B-40 B-41 B-42 201006383

The final EA and the judgment 98·014_01ρ-com 97-342-01p-com 97-339-0 lp_com 97-336-01p a com 97-287-01p-com BO o έ <N 97-205-0 lp-com Prepare EA**** or Risk Assessment FR Notification Transformation Results or Line A5547-127 i_ 〇oo f oo ίο RBMT15-101, SEMT15-02, SEMT15-15 T-120-7 5345 CBH-351 T45 Transgenic Gene Phenotype Glufosinate-tolerant and amoxicillin-tolerant CPB and PVY-resistant glufosinate-resistant lepidopteran-resistant glufosinate-tolerant and lepidopteran-resistant glufosinate-resistant Project Soybean Corn and Potato Equipment Hi Rapeseed Organisms (Monsanto) Acre Evo Pioneer Monsanto AgrEvo Monsanto AgrEvo Aike Evo Extension Application No. 96-068-Olp Application 1_ 98-014-01p 97-342-0 lp 97-339-01p 97-336-01p 97-287-0 lp 97-265-01p 97-205-0 lp B-43 B-44 B-45 B-46 B-47 B-48 B-49 • Lz, 201006383 Final EA and Judgment 97-204-0 lp_com 97-148-01p-com 97-099-0 lp_com 97· 013·01ρ —com 97-008-01p_com 96-317-0 lp_com Prepared or Risk Assessment FR Notification Transformation Results or Lines RBMT21-129 and RBMT21-350 RM3-3 > RM3-4, RM3-6 GA21 31807 and 31808 Lines G94-1 , G94-19, G-168 MON802 transgenic phenotypes of type CPB and PLRV resistant male instability glyphosate-tolerant bromoxynil-tolerant and lepidopteran-resistant type oil-type glyphosate Tolerance and ECB Resistant Control Projects Maize Maize Maize Cotton Soybean Maize (AgrEvo) Monsanto Bijo (Bejo) Monsanto Calgene DuPont (u Pont) Monsanto Extension Application No. *** Application 97-204-0 lp 97-148-01p 97-099-01p 97-013-01p 97-008-01p 96-317-01p B-50 B-51 B-52 B- 53 B-54 B-55 201006383

! 1 Final ΕΑ and judgment 1 96-291-01 PC〇m 96-248-01 p-com 96-068-0 lp-com 96-051-01p_com 96-017-0 lp_com 95-3 52-0 lp_com ε ο ο 〇! Τ~Η Ο 00 m rn tn CS Preparation **** or risk assessment 'FR notification i 1 1 transformation result or strain DBT418 1 strain plus FLAVRSAVR strain f CO , , CS 1 ί> Ύ (N rn , 4 r^· s ^ ° ^ ^ <<< 55-1 ' 63-1 ΜΟΝ 809 and ΜΟΝ 810 CZW-3 ν〇^ ί k ^ PQ ' o < Bu ® t Zhu Transgenic Gene Phenotype Europe Corn borer resistant type change fruit mature grass glufosinate tolerant PRSV resistant type European corn borer resistant type CMV, ZYMV, WMV2 resistant type CPB resistant type control item corn Mm soybean papaya corn pumpkin potato body Deca (DeKalb) Calgene AgrEvo Monsanto Asgrow Monsanto Extension Application No. *** 92-196-01p 95-093-01p Application 1 96 -29l-01p 96-248-0 lp 96-068-Olp 96-051-01ρ 96-017-0 lp 95-352-01p 95-338-01p B-56 B-57 B-58 Β-59 B- 60 B-61 B-62 -6ε_ 2010063 83

Final ΕΑ and judgment 95-324-0 lp-com 95-256-01p_com 95-228-0 lp-com 95-195-01p one com 95-179-0 lp_com 95-145-01p_com 95-093_01p_com 95_053·01ρ— Com report ΕΑ**** or risk assessment FR notification transformation results or strain cn t-^ m 1 35 IN 19-51a MS3 Bill 2 strain plus FLAVRSAVR strain B16 MON 80100 8338 transgenic phenotype alters fruit ripe sulfonylurea Tolerant males, European corn borer, resistant type, fruit ripening, glufosinate-tolerant lepidopteran resistance-type fruit control project. «l/frf cotton νή corn. 4fm corn maize M/n* institution 1_ ang Agritope Du Pont Plant Genetic Systems Northrup King Calgene DeKalb Monsanto 1 Monsanto Extension Application Number *** 92- 196-Olp Application 95-324-01p 95-256-01p 95-228-01p 95-195-01p 95-179-01p 95-145-01p 95-093-Olp 95-053-Olp B-63 B- 64 B-65 B-66 B-67 B-68 B-69 B-70 -〇寸-ο 201006383

1 Final EA and Judgment 95-045-01p_com B ο ο d! ο ό s 1 ON 94-357-0 lp_com 94-319-0 lp_com 94-308-0 lp_com 94-290-0 lp-com 94-257- 01p_com Preparatory EA**** or Risk Assessment 94-257-01p_ea FR Notification 10-Mar-1995 Conversion Results or Line 1445 ' 1698 20 strain plus FLAVRSAVR strain T14, T25 strain 176 531, 757, 1076 r cd Q BT6, BT10 , BT12, BT16, BT17, BT18, BT23 transgenic phenotype mature glyphosate and ten-type change fruit mature grass ammonium phosphine-resistant lepidopteran resistant lepidopteran resistant type reduced fruit polygalacturonase Content Coleoptera Resistance Control Project Cotton Cold D Corn Corn Cotton. «l/fff Potato Institute (Monsanto) Monsanto Calgene AgrEvo Ciba Seeds Monsanto +屮§ $ θ缌g 2 Monsanto Extension Application No. *** 92-196-01p Application 95-045-01p 95-030-01p 94-357-0lp 94-319-01p 94-308 -01p 94-290-0 lp 94-257-0 lp B-71 B-72 B-73 B-74 B-75 B-76 B-77 • One inch - 201 006383 Final EA and Judgment 94-230-0 lp_com 94-228-01p-com 94-227-0lp One Com 94-090-0 lp_com 93-258·01ρ-com 93-196-0 lp_com 92-204-0 lp_com 92-196-0 lp_com Preparatory EA**** or Risk Assessment 94-228-0 lp_ea 92-204-01p_ea FR Notice 24. Jan 95 3-Oct-1994 22. Feb 94 13-Dec-1994 19-Oct- 1992 Transformation results or strain 9 strains plus FLAVRSAVR strain 1345-4 strain N73 1436-111 pCGN3 828-21 2/86-18 and 23 40-3-2 BXN ZW-20 FLAVR SAVR trans-lean gene phenotype changes fruit ripening altered fruit Maturity changes fruit ripening changes oil type glyphosate-tolerant bromoxynil-tolerant WMV2 and ZYMV resistance-type changes fruit ripening control project Fanyou cUisr ^5» rapeseed soy cotton pumpkin plant Calgene DNA Plant Tech Calgene Calgene Monsanto Calgene Upjohn Calgene Extension Application Number (4) 92-196-01p 92-196 -01p Application 94-230-01p 94-228-0 lp 94-227-01p 94-090-0 lp 93-258-Olp 93-196-01p 92-20 4-0 lp 92-196-0 lp B-78 B-79 B-80 B-81 B-82 B-83 B-84 B-85 ©ο 201006383 According to the invention, 'isotianil' can be equipped In various formulations, it is clear that the examples include wettable powders, granulated wettable powders, water-cooled powders, liquid preparations, AL preparations, aqueous suspensions, and microcapsule preparations. = I can use a coated seed preparation. These preparations can be prepared according to known methods + such as by D-Dani and the exhibition (eg liquid or solid diluent or manned and: when needed) with surfactants (eg emulsifiers and / or dispersing agents) Mixed

HI uses water as an expansion device, and for example, an organic solvent can be used as an auxiliary solvent. Liquid dilutions or carriers include, for example, aromatic fumes (eg, xylene, aromatic fumes, or vaporized aliphatic hydrocarbons (eg, benzene, vaporized, by class [eg, cyclohexane, and chain hydrocarbons (eg, Stone and strong polar solvents (such as bis-p-isobutyl, and cyclohexanone) 'Golden bear Sujing ^ • Monomethyl armoramine and dimethyl succinium) and water. Soil, viscosity:, talc package such as: natural Mineral grinding - synthetic mineral milling (such as di-soil, montmorillonite, algae), solid carrier including, for example, acid, I soil and Wei salt). Granule marble, pumice, wave η a knife broken natural stone (for example: calcite, granules, organic matter fine 'inorganic or organic milled synthetic rods.') (eg, mineral chips, coconut shells, ears of corn and tobacco surfactants include, for example, non-separating [eg: polyoxyethylene] _ ^Ionic surfactant aryl aryl poly-, oxyethylene ester (four) _ (for example: κ acid alkyl S 曰, alkyl sulfate and sulfonic acid aryl • 43- 201006383 ester), and albumin hydrolysis Products, etc.. The dispersing agent includes, for example, lignin sulfite waste liquid and sulfhydryl cellulose. A binder may also be used in the preparation (powder, granules and emulsion), and examples thereof include carboxymethyl cellulose, natural or synthetic polymers (for example, gum arabic, polyvinyl alcohol and polyvinyl acetate). Coloring agents, examples of which include: inorganic pigments (for example: iron oxide, titanium oxide and Prussian blue), organic dyes (such as alizarin dyes, azo dyes or metal ouocyanine dyes), and trace elements such as iron and , butterfly, copper, cobalt, molybdenum and zinc salts. The formulation usually comprises 0.01 to 95% by weight of dandan, preferably between 0.1 and 90% by weight. When treating seeds, the dosage of dandan may be From 0.001 to 50 g per kg of seed, preferably from 0.01 to 10 g per kg of seed. The preparation and use of the compound of the present invention are explained in more detail below with reference to the following examples. However, the invention should not be limited thereto. Test and preparation example Test compound 3,4-dichloro-2^cyano-1,2-thiazol-5-indoleani (common name: isotianil) herbicide (common name) H-1 :fen Fenoxaprop-P-ethyl H-2 : alkyne π oxadiargyl (test herbicide preparation method) -44 - 201006383 Carrier: two ketone surfactant: Park 5 injury reset ratio by the above amount of dance j and ^ methyl oxygen poly _ 1 part by weight Mix, add the water to dilute the obtained preparation to mix with the dandan (1 part by weight) seed treatment method: Danny water suspension ° ° use a blender to wrap the predetermined seed (° mouth name, Nihonbare), the above After the Danny water suspension, air dry conditioning.

Test Example Effect of low herbicidal compounds on phytotoxicity of rice The rice seeds treated with pre-d, d-danny water treatment were planted in plastic pots (1 cm 2 ) filled with 7 paddy soil and then grown in a greenhouse. When the plant reaches the 2.5 leaf stage, a predetermined amount of the test herbicide dilution is sprayed from above. One day after the treatment, the plants were irrigated to 3 cm. After 3 weeks of treatment, the phytotoxicity of the rice plants was determined. When analyzing the phytotoxicity of rice plants, 100% showed complete death, and 〇% showed no phytotoxic effect. The results are summarized in Table 1 below. Table 1 Herbicidal active compound (g/ha) Isotianil (g/l〇〇Kg seed) 0 200 400 Benefit 0 0 10 Hl(lOO) 60 20 20 H-2(60) 90 90 60 -45 - 201006383 登J._Patent specification: —:.......:. (The format and order of this manual, please do not change it at all, please do not fill in the ※ part)

※Application number: Office ※Application date: Public^pc classification: 1. Invention name: (Chinese/English) Method for reducing phytotoxicity METHOD OF REDUCING PHYTOTOXICITY II. Abstract of the invention: The present invention relates to a reduction of seed crops A method of phytotoxicity in which seeds are treated with 3,4-dichloro-2'-cyano-1,2-thiazol-5-nonanilide prior to sowing. III. English invention summary: A method of reducing phytotoxicity to seed crops by treating the seeds with 3,4-dichloro-2'-cyano-l,2-thiazole-5-carboxanilide before seeding. 201006383 VII. Patent application scope: 1 A method of reducing phytotoxicity to a seed crop by treating the seed with 3,4_dioxa-2 cyano-1,2-thiazol-5-nonanilide. 1 2 According to the method of the scope of the patent application, wherein the method is: and the method is to reduce the planting of rice (4) after direct sowing, and the method is in the seedling, wherein the seed crop For water 4. Toxicity. Reduce the seedlings of the seedlings during the nursery period. And the method is transplanted into the field, f, wherein the seed crop is water toxic. * Reduce the plant on rice seedlings ❹ -46 - 201006383 IV. Designated representative map: (1) The representative representative of the case is: (No). (2) A brief description of the symbol of the representative figure: None 5. If there is a chemical formula in this case, please disclose the chemical formula that best shows the characteristics of the invention: None