CN103999870A - Fungicidal composition - Google Patents

Abstract

The invention provides a fungicidal composition. The fungicidal composition comprises bellkute and iprodione which are taken as active components, wherein the bellkute and the iprodione are capable of achieving the effect of synergies in a weight ratio range being (100:1)-(1:100). The weight of the active components comprising the bellkute and the iprodione accounts for 5%-90% of the weight of the composition. According to the fungicidal composition, bellkute and iprodione are subjected to binary compounding; bellkute and iprodione are different in action mechanisms and free of cross resistance; bellkute and iprodione are mixed for use, so that the bacteriocidal spectrum can be expanded, and good prevention and treatment effects can be achieved; meanwhile, the production of drug resistance of the pathogenic bacteria is reduced, thus the fungicidal composition is relatively safe for various crops and can be used for preventing and treating fungus on cereals, fruits, vegetables, cottons, fruit trees and ornamental plants.

Description

A kind of bactericidal composition

Technical field

What the present invention relates to is a kind of bactericidal composition, the binary built relating in particular to, the bactericidal composition that is applied to agricultural plant chemoproection field, and said composition shows the bactericidal activity of Synergistic to harmful fungoid in crops or gardening.

Background technology

Due to the harm of various phytopathogens, many crops are brought about great losses, the lighter's underproduction, severe one can cause total crop failure, directly affects peasant's economic interests, therefore forces peasant to have to prevent and treat disease with bactericide.

The multiple fungal disease of the upper often generation of agricultural crops (Cereal, melon and fruit, vegetables, flowers), as gray mold, powdery mildew, stalk break, stem wilt, climing rot, anthracnose, ring spot, scab, leaf spot, spot defoliation, fruit soft rot, penicilliosis, green mould, rust, peach brown rot, smut, cecospora spot etc.

Hundred can obtain (Bellkute), have another name called biguanides three octyl benzene sulfonates, and its chemical name is: 1 ＇ 1-imino group (octyl group methylene) biguanides 3(alkyl benzene sulfonate), be the antistaling disinfectant of the single molecular structure of biguanides salt, its structural formula is:

Hundred can be produced by Japanese Cao Da company.Hundred can tagging and prevention type bactericide as broad spectrum activity, local permeability is strong, some disease fungus is had to very high growth inhibitory activity, mainly the biosynthesis to Mycophyta ester compounds and cell membrane function work for they, suppress the formation of spore germination, germ tube elongation, appresorium and mycelia, can prevent and treat the fungal disease being caused by most of sac fungis and imperfect fungus.Hundred can obtain the disease such as gray mold, powdery mildew, stalk break, stem wilt, climing rot, anthracnose, ring spot, scab, leaf spot, spot defoliation, penicilliosis, green mould and fruit soft rot that can be widely used in fungal disease, particularly fruit, vegetables and multiple kinds of crops that control most of sac fungis and imperfect fungus cause.Especially to tomato, grape grey mould, apple tree spot defoliation, powdery mildew of cucumber, the climing rot of watermelon all have good preventive effect.

Iprodione (Iprodione) is the dicarboximide series bactericidal agent of being developed by Beyer Co., Ltd, molecular formula is C13H13Cl2N3O3, and chemical name is 3-(3,5-dichlorophenyl)-N-isopropyl-2,4-dioxo imidazoline-1-carboxylic acid amides, is disclosed in US3823240.Its structural formula is:

Iprodione is a kind of wide spectrum contact bactericide, the gray mold that is mainly used in preventing and treating the crops such as fruit tree, vegetables, melon and fruit class.It can Profilin kinases, control the thin intracellular signal of many cell functions, comprise that carbohydrate is in conjunction with the interference effect that enters fungi component.Therefore it both can Antifungi spore germination and generation, also can suppress mycelial growth.All there is to impact the each developmental stage in pathogen history of life, can prevents and treats the fungi that benzimidazole systemic fungicide is had to resistance.Main controlling object is various crop, fruit tree and the fruit storage phase disease that Botrytis, Monilia, Penicillium, Sclerotinia, Alternaria, Helminthosporium etc. cause.

In the time of practical application, a kind of reactive compound of long-term single administration is prevented and treated disease and can be caused disease drug resistance to produce, and causes that compound preventive effect declines, and even thoroughly loses preventive effect.The risk developing immunity to drugs in order to reduce phytopathogen, it is one of way of normal employing at present that application Synergistic bactericidal composition is prevented and treated noxious plant disease fungus.

Summary of the invention

One object of the present invention is to provide a kind of bactericidal composition for above-mentioned weak point, is a kind of bactericidal composition with synergistic function.Another object of the present invention is to provide a kind of method of preventing and kill off plant disease.

The present invention is through various further investigations, found that contain hundred can with the bactericidal composition of iprodione, can prevent and treat the fungal disease of various vegetables, fruit and cereal crop.Containing hundred can be with the bactericidal composition of iprodione not only for common phytopathogen, and the pathogen that those have been produced to drug resistance is also very effective, recurs and under condition, also has good control efficiency, thereby completed the present invention in disease.

A kind of bactericidal composition of the present invention is to take following technical scheme to realize:

A kind of bactericidal composition, comprising hundred can be with iprodione as active component, and described hundred can be 100:1-1:100 with the weight ratio of iprodione.

Hundred can be simultaneously with iprodione, together or separate administration, or uses successively; Order in separate administration situation conventionally on the result of prophylactico-therapeutic measures without any impact.

Compound hundred can be with iprodione conventionally with 100:1-1:100, preferably 50:1-1:50, and more preferably 25:1-1:25, more preferably 10:1-1:10, more preferably the weight ratio of 5:1-1:5 is used.

A kind of bactericidal composition, comprise hundred can with iprodione active component, filler and/or surfactant.

Described bactericidal composition formulation is wetting powder, missible oil, suspending agent, suspension emulsion, microcapsules, seed coat agent, microemulsion, aqueous emulsion, water dispersible granules, foaming agent, paste, aerosol, ultra low volume spraying liquid.

Described bactericidal composition, wherein containing hundred 5%-90% that can account for the content of iprodione active component bactericidal composition.

Described bactericidal composition, wherein containing hundred 10%-80% that can account for the content of iprodione active component bactericidal composition.

Described bactericidal composition, wherein containing hundred 20%-60% that can account for the content of iprodione active component bactericidal composition.

Prevent and kill off a method for plant disease, described bactericidal composition is applied to plant, plant propagation material and the organ growing subsequently, environment.

The propagating materials of the plant that described compositions-treated needs protection.

The plant propagation material of described compositions-treated.

The purposes of described bactericidal composition for preventing and treating fungi.

The purposes of described bactericidal composition for preventing and treating fungi on Cereal, fruit, vegetables, cotton, fruit tree, ornamental plants.

Described bactericidal composition is used for preventing and treating gray mold, stalk break, leaf spot, banded sclerotial blight, full rot, rust, anthracnose, early blight, powdery mildew, scab, black spot, climing rot, spot defoliation, stem wilt etc.

Described bactericidal composition is for preventing and treating the purposes of fruit vegetables storing period disease.

Described bactericidal composition can dressing, spraying, immersion, dense fog, the mode such as broadcast sowing or pour uses.

Described hundred can use simultaneously or use respectively or sequential application with iprodione compound.

Detailed Description Of The Invention

Hundred can with the synergy of iprodione, the weight ratio of reactive compound can be selected in larger scope.Hundred can be 100:1-1:100 with the weight ratio of iprodione, or 50:1-1:50, or 20:1-1:20, preferably 10:1-1:10, more preferably 5:1-1:5.

Bactericidal composition of the present invention, the content of active component depends on dosage when sterilization is used separately, also depends on mixed ratio and the synergistic effect degree of a kind of bactericide and another kind of bactericide.

In described bactericidal composition, hundred can account for the content of iprodione active component the 5%-90% of bactericidal composition, preferably 10%-80%, more preferably 20%-60%.

In described bactericidal composition, active component be can only comprise, filler and/or surfactant also can be comprised.

Bactericidal composition of the present invention, can be prepared into various formulations, for example wetting powder, missible oil, suspending agent, suspension emulsion, microcapsules, seed coat agent, microemulsion, aqueous emulsion, water dispersible granules, foaming agent, paste, aerosol, ultra low volume spraying liquid.

According to the present invention, term " filler " refers to can be combined with reactive compound or for example combine, so that it is easier to be administered to the natural or synthetic organic or inorganic compound of object (plant, crop or careless class).Therefore, described filler is preferably inertia, at least should be agricultural acceptable.Described filler can be solid or liquid.

Liquid filling agent is generally: water, alcohol type (for example methyl alcohol, ethanol, isopropyl alcohol, butanols, ethylene glycol etc.), ketone (for example acetone, methyl ethyl ketone, isobutyrone, cyclohexanone etc.), ethers (for example ether, dioxanes, methylcellulose, oxolane etc.), aliphatic hydrocarbon class (for example kerosene, mineral wet goods), aromatic hydrocarbons class (for example benzene, toluene, dimethylbenzene, solvent naphtha, Fluhyzon, chlorinated aromatic hydrocarbons, chlorinated aliphatic hydrocarbon, chlorobenzene, Deng), halogenated hydrocarbon class, amide-type, sulfone class, dimethyl sulfoxide (DMSO), mineral and vegetable oil, animal wet goods.

Solid-filling agent is generally: phyteral powdery type (for example particle of soybean meal, starch, grain dust, wood powder, bark powder, sawdust, walnut shell powder, wheat bran, cellulose powder, coconut husk, corncob and tobacco stem, extract residue after plant essence etc.), paper, sawdust, pulverize synthesized polymer body, clay class (such as kaolin, bentonite, acid china clay etc.), the talcum powder class of synthetic resin etc.Silica (for example diatomite, silica sand, mica, moisture silicic acid, calcium silicates), chemical fertilizer, the clay fertilizer of active carbon, natural mineral matter class (float stone, attapulgite and zeolite etc.), the inorganic mineral powder of firing diatomite, sand, plastics medium etc. (such as polyethylene, polypropylene, polyvinylidene chloride etc.), potassium chloride, calcium carbonate, calcium phosphate etc., ammonium sulfate, ammonium phosphate, urea, greening ammonium etc., these materials can use separately or two or more is mixed.

For making the emulsification of active ingredient compound, disperse, solubilization, and/or wetting can use surfactant for example can enumerate fatty alcohol-polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene high-grade aliphatic ester, the phosphate of polyoxyethylene alcohol or phenol, the fatty acid ester of polyalcohol, alkyl aryl sulphonic acid, naphthalene sulfonic acids polymer, lignosulfonates, a shape copolymer of macromolecule comb shape, butyl naphthalene sulfonate, alkylaryl sulfonates, alkyl sodium sulfosuccinate, grease, fatty alcohol and ethylene oxide condensate, the polyacrylates such as alkyltaurate, protein hydrolysate.Suitable oligosaccharide thing or polymer, the combination of for example vinyl monomer based on independent, acrylic acid, polyoxyethylene and/or polyoxypropylene or itself and for example (polynary) alcohol or (polynary) amine.

In preparation, also can use such as carboxymethyl cellulose of tackifier, and the natural and synthetic polymer of powder, particle or latex form, for example gum Arabic, polyvinyl alcohol and polyvinyl acetate or natural phospholipid, for example cephalin and lecithin, and synthetic phospholipid.Other additive is mineral oil and vegetable oil.

The colouring agent that may use is as inorganic pigment, for example, iron oxide, titanium oxide and Prussian blue, and organic dyestuff, as alizarin dyes, azo dyes or metal phthalein mountain valley with clumps of trees and bamboo dyestuff, and trace nutrient, as iron, manganese, boron, ketone, cobalt, molybdenum and zinc salt.

The disintegrant that may use is selected from: one or more in bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, sodium bicarbonate.

The stabilizing agent that may use is selected from: the one in sodium citrate, resorcinol.

The antifreezing agent that may use is selected from: one or more in ethylene glycol, propane diols, glycerine, urea.

Described defoamer is selected from: silicone oil, silicone compound, C _10-20saturated fat acid compounds, C _8-10one or more in aliphatic alcohols compound.

Optionally, also can comprise other annexing ingredient, for example protecting colloid, adhesive, thickener, thixotropic agent, bleeding agent, stabilizing agent, screening agent.

Described preparation of the present invention can be mixed described active component to prepare with filler and/or surfactant by known way, also can add other conventional additives, as drier and colouring agent, stabilizing agent, pigment, defoamer, preservative, thickener etc.

Bactericidal composition of the present invention can also have with other medicament of weeding, desinsection or bactericidal property, particularly mixes use with protective fungicide, also can mix use with insecticide, protective agent, growth regulator, nutrient for plants or soil conditioner.

Bactericidal composition of the present invention has extraordinary fungicide performance, and can be used to prevent and treat phytopathogenic fungi, described fungi is especially selected from Ascomycetes (Ascomycetes), Basidiomycetes (Basidiomycetes), Phycomycetes (Phycomycetes) and deuteromycetes (Deuteromycetes) fungi.

Bactericidal composition of the present invention is particularly suited for preventing and treating following plant disease:

Chain lattice spore (AIternaria) on-vegetables, oilseed rape, sugar beet, fruit and rice belongs to,

Silk capsule mould (Aphanomyces) on-sugar beet and vegetables belongs to,

Bipolaris (Bipolaris) in-corn, Cereal, rice and lawn belongs to and interior navel is wriggled, and spore (Drechslera) belongs to,

Standing grain powdery mildew (Blumeria graminis) (powdery mildew) on-Cereal,

Botrytis cinerea (Botrytis cinerea) (gray mold) on-strawberry, vegetables, flowers and grape vine,

Lettuce dish stalk mould (Bremia lactucae) on-lettuce,

Tail spore (Cercospora) on corn, soybean, rice and sugar beet belongs to,

(for example the standing grain on Cereal revolves spore to cochliobolus (Cochliobolus) genus on-corn, Cereal, rice

Chamber bacterium (Cochliobolus sativus), the palace portion cochliobolus (Cochliobolus miyabeanus) on rice),

Thorn dish spore (Colletotricum) on-soybean and cotton belongs to,

Interior navel on-Cereal and corn wriggle spore (Drechslera) belong to,

Prominent navel on-corn wriggle spore (Exserohilum) belong to,

. two spore powdery mildews (Erysiphe cichoracearum) and monofilament shell powdery mildew on cucumber

(Sphaerotheca fuliginea) ,

Fusarium (Fusarium) on-each Plants belongs to and wheel branch spore (Verticillium) belongs to,

Gaeumannomyce (Gaeumanomyces graminis) on-Cereal belongs to,

Red mould (Gibberella) on-Cereal and rice belongs to the (for example gibberella fujikuroi (Gibberella on rice

fujikuroi)) ,

Gram’s staining complex (Grain staining complex) on-rice,

Length on-corn and rice wriggle spore (Helminthosporium) belong to,

Michrodochium nivale on-Cereal,

Ball chamber bacterium (Mycosphaerella) on-Cereal, banana and peanut belongs to,

Yam bean layer rest fungus (Phakopsora pachyrhizi) on-soybean and mountain horse sulphur layer rest fungus

(Phakopsara meibomiae) ,

Phomopsis (Phomopsis) on-soybean and sunflower belongs to,

Phytophthora infestans (Phytophthora infestans) on-potato and tomato,

The raw single shaft of grape mould (Plasmopara viticola) on-grape vine,

Apple mildew bacterium (Podosphaera leucotricha) on-apple,

Wheat-based maize ear rot bacterium (Pseudocercosporella herpotrichoides) on-Cereal,

False downy mildew (Pseudoperonospora) on-lupulus and cucurbit belongs to,

Handle rest fungus (Puccinia) on-Cereal and corn belongs to,

Nuclear cavity bacteria (Pyrenophora) on-Cereal belongs to,

Pyricularia oryzae (Pyricularia 0 η zae), Bamboo grass wood photovoltaicing leather bacteria (Corticium sasakii), broom stalk Cylindrocarpon (Sarocladium oryzae), sheath rot of rice plant (S.attenuatum), rice leaf smut (Entyloma oryzae) on-rice

Piricularia oryzae (Pyricularia grisea) on-lawn and Cereal,

-lawn, rice, corn, cotton, oilseed rape, sunflower, sugar beet, vegetables and other are planted

Corruption mould (Pythium) on thing belongs to,

-cotton, rice, potato, lawn, corn, oilseed rape, potato, sugar beet, vegetables and its

Rhizoctonia (Rhizoctonia) on his plant belongs to,

Sclerotinite (Sclerotinia) on-oilseed rape and sunflower belongs to,

Wheat septoria (Septoria tritici) on-wheat and the many spore (Stagonospora of clever withered shell

nodorum) ,

Grape snag shell (Erysiphe) on-grape vine,

Setospaeria on-corn and lawn belongs to,

Silk axle smut (Sphacelotheca reilinia) on-corn,

Thielaviopsis sp (Thievaliopsis) on-soybean and cotton belongs to,

Tilletia foetida (Tilletia) on-Cereal belongs to,

Smut (Ustilago) on-Cereal, corn and sugar beet belongs to,

Black star bacterium (Venturia) on-apple and pears belongs to (scab).

Bactericidal composition of the present invention is even more important to preventing and treating a large amount of fungies various crops for example, as cotton, vegetable variety (cucumber, beans, tomato, potato and cucurbitaceous plant), barley, dogstail, oat, banana, coffee, corn, fruit variety, rice, rye, soybean, grape vine, wheat, ornamental plants, peanut, rape, sugarcane and various crop seed in.

Bactericidal composition of the present invention is particularly suitable for preventing and treating following plants pathogenic epiphyte: the standing grain powdery mildew (Blumeria graminis) (powdery mildew) in Cereal, two spore powdery mildews (Erysiphe cichoracearum) in cucurbitaceous plant and monofilament shell (Sphaerotheca fuliginea), apple mildew bacterium (Podosphaera leucotricha) in apple, grape snag shell (Uncinula necator) in grape vine, handle rest fungus (Puccinia) in Cereal belongs to, cotton, rhizoctonia (Rhizoctonia) in rice and lawn belongs to, smut (Ustilago) in Cereal and sugarcane belongs to, venturia inaequalis (Venturia inaequalis) (scab) in apple, length in Cereal wriggle spore (Helminthosporium) belong to, wheat glume blight bacterium (Septoria nodorum) in wheat, strawberry, vegetables, Botrytis cinerea (Botrytis cinerea) (gray mold) in ornamental plants and grape vine, peanut tail spore (Cercospora arachidicola) in peanut, eye spot bacterium (PseudocercosporellaHerpotrichoides) in Wheat and barley, Pyricularia oryzae (Pyricularia oryzae) in rice, phytophthora infestans (Phytophthora infestans) in potato and tomato, grape single shaft mould (Plasmopara viticola) in grape vine, stalk break (sclerotiose) in rape, false downy mildew (Pseudoperonospora) in lupulus and cucumber belongs to, chain lattice spore (Alternaria) in vegetables and fruit belongs to, ball chamber bacterium (Mycosphaerella) in banana belongs to and Neurospora (Fusarium) and wheel branch spore (Verticillium) genus.

Preferably, bactericidal composition of the present invention can be used for preventing and treating gray mold on Cereal, fruit, vegetables, cotton, fruit tree, ornamental plants, stalk break, leaf spot, banded sclerotial blight, full rot, rust, anthracnose, early blight, powdery mildew, scab, black spot, climing rot, spot defoliation, stem wilt etc.

Bactericidal composition of the present invention also can be used for preventing and treating fruits and vegetables storage period disease.The fruit rot for example being caused by following pathogene:

Aspergillus bacterium, for example aspergillus flavus;

Botrytis (Botrytis) bacterial classification, for example Botrytis cinerea (Botrytis cinerea);

Penicillium (Penicillium) bacterial classification, for example penicillium expansum (Penicillium expansum) and P.purpurogenum;

Sclerotinia (Sclerotinia) bacterial classification, for example sclerotinite (Sclerotinia sclerotiorum);

Verticillium (Verticilium) bacterial classification, for example black and white wheel branch spore (Verticilium alboatrum).

Bactericidal composition of the present invention also can be used for control kind and passes and soil-borne disease.The kind for example being caused by following pathogene passes and soil passes rotten and wilting disease and seedling disease:

Alternaria belongs to plants, for example raw chain lattice spores of rape (Alternaria brassicicola);

Aphanomyces (Aphanomyce) belongs to plants, for example mould (Aphanomyces of Kidney bean silk capsule

euteiches) ;

Ascochyta (Ascochyta) belongs to be planted, for example Ascochyta lentis;

Aspergillus, for example aspergillus flavus;

Cladosporium belongs to plants, for example draft branch spore (Cladosporium herbarum);

Cochliobolus belongs to plants, for example standing grain cochliobolus;

(conidial form: Drechslera, Syn: the long spore bacterium that wriggles);

Colletotrichum belongs to be planted, for example hair core anthrax-bacilus (Colletotrichum coccodes);

Fusarium belongs to plants, for example yellow sickle spore;

Gibberella belongs to plants, and for example beautiful another name for Sichuan Province multitude is red mould;

Shell ball spore belongs to (Macrophomina) genus kind, for example Kidney bean shell ball spore (Macrophomina

phaseolina) ;

Little setting-out shell belongs to bacterial classification, for example, avenge rotten little setting-out shell;

Penicillium belongs to plants, for example penicillium expansum;

Phaeosphaeria bacterial classification, for example Phaeosphaeria nodorum;

Phoma (Phoma) belongs to plants, for example black blastema point mould (Phoma lingam);

Phomopsis (Phomopsis), for example soybean Phomopsis (Phomopsis sojae);

Phytophthora belongs to plants, for example Phytophthora cactorum (Phytophthora cactorum);

Nuclear cavity Pseudomonas belongs to be planted, for example wheat class nuclear cavity bacteria (Pyrenophora graminea);

Pyricularia Sacc. (Pyricularia) belongs to plants, for example Magnaporthe grisea (Pyricularia oryzae);

Pythium kind, for example Pythium ultimum;

Rhizoctonia belongs to plants, for example Rhizoctonia solani Kuhn;

Rhizopus (Rhizopus) belongs to plants, for example Rhizopus oryzae (Rhizopus oryzae);

Bulbil belongs to (Sclerotium) genus kind, for example Sclerotium rolfsii (Sclerotium rolfsii);

Core coral Pseudomonas (Typhula) belongs to be planted, for example meat spore core coral bacterium (Typhula incarnata);

Verticillium (Verticillium) belongs to plants, for example verticillium dahliae (Verticillium dahliae);

Bactericidal composition of the present invention, under control phytopathogenic fungi desired concn, the compatibility having had with plant.Therefore, can be applied to plant, plant propagation material and the plant organ growing subsequently, environment, habitat or storage areas when bactericidal composition of the present invention.

Term " plant propagation material " is interpreted as referring to all plant parts that have fertility, for example seed, and it can be used for breeding the latter, and for example cuttage bar of vegetable matter or stem tuber (for example potato).Therefore, plant part used herein comprises plant propagation material.What can mention is for example seed (narrowly), root, fruit, stem tuber, bulb, rhizome and plant part.Also can mention until the germination plant suppressing after germinateing soil or after emerging and effective plant.Immature plant can before transplanting, be undertaken all by dipping or Local treatment is protected.

The plant organ growing is subsequently any part by the plant propagation material plant that for example seed produces.Plant part, plant organ and plant also can be benefited from by active agent combinations or composition are applied to plant propagation material protective plant and avoid plant disease.Certain plants part and some plant organ growing subsequently also can be regarded plant propagation material as, himself can use with bactericidal composition (or processing); Thereby the plant, other plant part and other the plant organ that are produced by treated plant part and treated plant organ also can be benefited from by bactericidal composition being applied to certain plants part and certain plants Organoprotective plant and avoid plant disease.

The environment of plant growth, habitat refer to the supporter that can make crops take root, grow, for example: soil, water etc., concrete raw material can use such as sand, float stone, vermiculite, diatomite, agar-agar, gelling material, polymer substance, asbestos, wood chip, bark etc.Preferably soil.

According to the present invention, use the processing that bactericidal composition of the present invention carries out plant directly to carry out according to conventional treatment method, or realize by processing its environment of living in, habitat or storage space, for example immersion liquid of described conventional treatment method, spraying, evaporation, atomizing, broadcast sowing, smear; For plant propagation material or the plant organ growing subsequently, especially seed, also can carry out one or more layers dressing.Type of service depends on the object being intended to; Under any circumstance all should guarantee that the present composition is meticulous as far as possible and distribute equably.Preferred application process is to be administered to plant shoot to divide, especially foliage applying; Application times and use biology and the weather life condition that ratio depends on objective microbe.Active component also can reach in plant corpus by root system via soil or water, can water location or this material is mixed in soil with solid form with liquid preparation thus.Bactericidal composition of the present invention also can be administered on seed-grain in seed treatment, and stem tuber or cereal can soak successively with the liquid preparation of every kind of active component thus, or with the wet or dry preparation parcel having mixed.

Active ingredient is mixed environment or the habitat at plant place, for example, mix (soil application) in soil with the form of particle, and active ingredient also can enter inside plants by root system.To the method for using medicament in soil, for example liquid preparation is diluted in water or does not dilute and be directly applied to the root of plant corpus or the medium method in rice seedling bed that seedling is used, granule is disseminated to method in the root of plant corpus or the rice seedling bed of seedling to be had and prior to seeding pulvis, water dispersible granules etc. is sprayed on to the method for mixing in soil and with soil entirety, before sowing or before serike body, will after pulvis, water-dispersible grain dilution agent, be sprayed in implantation hole, seed furrow, then the method for sowing etc.

Bactericidal composition of the present invention for the protection of plant propagation material or the plant organ growing subsequently for example seed, fruit, stem tuber or kernel or plant cutting be not subject to infecting of plant disease; can be before using for example, with this propagating materials of compositions-treated or the plant organ that grows subsequently, seed dressing prior to seeding.Also can be by soaking kernel or with the coated kernel of solid composite, active component is applied in kind of benevolence and (is coated with) in fluid composition.In the time propagating materials being applied to for example seed in seeding process and broadcasting in ditch, also said composition can be applied to application site.These plant propagation materials to the processing method of plant propagation material and so processing are all the further themes of the present invention.

In the control of plant disease, hundred can with iprodione separately co-administered or hundred can with the composition of iprodione use by before or after plant seeding or plant seed, plant or soil spraying or dusting are carried out before or after emerging.Composition of the present invention can be used before or after plant or plant propagation material are by infection process.

Product of the present invention can finished product preparation form provide, and in bactericidal composition, each material mixes, and the composition of bactericidal composition also can single dose form provide, and before use, directly in bucket or in tank, directly mixes, and is then diluted to required concentration.The frequency of administration of bactericidal composition of the present invention and amount of application, according to the mixing ratio of active ingredient, meteorological condition, medicament form, use period, application process, use place, prevent and kill off the differences of target pest, target crops etc. and variant:

-for the concentration of leaf portion processing active substance be conventionally: 10-600g/ha, preferably 30-500g/ha, more preferably 50-300g/ha; For dipping or instil use for, described dosage even can also reduce, particularly in the time using inert base as asbestos or pearl rock;

-for seed treatment: 10-6000g active substance/100kg seed, preferably 50-5000g active substance/100kg seed;

-for soil treatment: 10-6000 g/ha, preferably 50-3000 g/ha;

-for fruits and vegetables impregnation process: dilute with water 200-2000 times liquid, soaks fruit one minute.

Above-mentioned dosage is only general exemplary dose, and when actual using, those skilled in the art can, according to actual conditions and needs, especially adjust rate of application according to the seriousness of the character of pending plant or fruits and vegetables and germ.

Bactericidal composition of the present invention can be used by common method, and amount of application is with weather or crop state variation, use optimum period and be crop susceptible before or the susceptible initial stage.Its lasting period is conventionally relevant with the content in bactericidal composition, also relevant to extraneous factor.

Bactericidal composition of the present invention has the following advantages:

1, hundred can with the two mechanism of action complementation of iprodione, can bring into play better protection and therapeutic action;

2, hundred can have obvious synergistic function with iprodione, reduce single every mu of using dosage of planting medicament, thereby both can reduce influence degree, and can significantly reduce again agricultural cost, the effect of even preventing and treating some disease exceedes existing other bactericide;

3, fungicidal spectrum is more extensive, to the disease being caused by sac fungi, basidiomycetes, imperfect fungus, Phycomycetes in various crop, has good control efficiency.

Embodiment

Below with reference to embodiment, the invention will be further described:

Example of formulations:

Can obtain+10% iprodione wettable powder of embodiment 1 2,%00

Hundred can obtain 2%

Iprodione 10%

Lauryl sodium sulfate 10%

Sodium lignin sulfonate 5%

White carbon 10%

Kaolin complements to 100%

Reactive compound, various auxiliary agent and filler etc. are mixed in the ratio composition of formula, after ultra-fine pulverizer disintegrating, obtain 2,%00 can obtain+10% iprodione wettable powder.

Can obtain+1% iprodione wettable powder of embodiment 2 50,%00

Hundred can obtain 50%

Iprodione 1%

Calcium dodecyl benzene sulfonate 1%

Sodium lignin sulfonate 2%

Sucrose complements to 100%

Reactive compound, various auxiliary agent and filler etc. are mixed in the ratio composition of formula, after ultra-fine pulverizer disintegrating, obtain 50,%00 can obtain+1% iprodione wettable powder.

Can obtain+4% iprodione missible oil of embodiment 3 1,%00

Hundred can obtain 1%

Iprodione 4%

Ethoxylated castor oil 5%

Calcium dodecyl benzene sulfonate 3%

Dimethyl sulfoxide (DMSO) complements to 100%

Mentioned component is proportionally prepared, stirred and obtain the phase of homogeneous.

Can obtain+5% iprodione wettable powder of embodiment 4 50,%00

Hundred can obtain 50%

Iprodione 5%

Lauryl sodium sulfate 10%

Sodium lignin sulfonate 5%

White carbon 10%

Kaolin complements to 100%

Said components is mixed in proportion, and grind, pulverize, be prepared into wetting powder.

Can obtain+50% iprodione water dispersible granules of embodiment 5 2,%00

Hundred can obtain 2%

Iprodione 50%

MODIFIED LIGNOSULPHONATE 5%

Lauryl sodium sulfate 5%

Urea 5%

Kaolin complements to 100%

By hundred can obtaining, iprodione, dispersant, wetting agent, disintegrant and filler mix in the ratio of formula, becomes wetting powder through air-flow crushing; Add again a certain amount of water to mix extruding and make material.After drying screening, obtain 2,%00 can obtain+50% iprodione water dispersible granules.

Can obtain+50% iprodione suspension emulsion of embodiment 6 0.5% hundred

Oil phase:

Hundred can obtain 0.5%

Methyl oleate 10%

Ethoxylated castor oil 5%

Water:

Iprodione 50%

The sodium salt 1% of the naphthalene sulfonic acids-formaldehyde condensation products of sulfonation

Water complements to 100%

Can be dissolved in methyl oleate hundred, add ethoxylated castor oil to obtain oil phase; According to formula, the sodium salt of the naphthalene sulfonic acids-formaldehyde condensation products of iprodione, sulfonation, water are obtained after grinding and/or high speed shear to the suspending agent of water iprodione; Under agitation add water to obtain suspension emulsion oil phase.

Can obtain+10% iprodione wettable powder of embodiment 7 10,%00

Hundred can obtain 10%

Iprodione 10%

Sodium lignin sulfonate 1%

NaLS 2%

The silicic acid 1% of high degree of dispersion

Kaolin complements to 100%

Said components is mixed in proportion, and grind, pulverize, be prepared into wetting powder.

Can obtain+50% iprodione coated granule of embodiment 8 40,%00

Hundred can obtain 40%

Iprodione 50%

Polyethylene glycol 3%

The silicic acid 1% of high degree of dispersion

Calcium carbonate complements to 100%

In blender, levigate Fungicidal active compound is evenly applied on the carrier being soaked by polyethylene glycol.Can obtain in this way dustless coated granule.

Can obtain+10% iprodione wettable powder of embodiment 9 50,%00

Hundred can obtain 50%

Iprodione 10%

Lauryl sodium sulfate 1%

Sodium lignin sulfonate 1%

Kaolin complements to 100%

Said components is mixed in proportion, and grind, pulverize, be prepared into wetting powder.

Can obtain+60% iprodione granule of embodiment 10 20,%00

Hundred can obtain 20%

Iprodione 60%

Sodium lignin sulfonate 4%

Carboxymethyl cellulose 2%

Kaolin complements to 100%

Reactive compound is mixed with auxiliary agent and grind, with water-wet, granulation, then dry in air stream.

Can obtain+2% iprodione seed coat agent of embodiment 11 50,%00

Hundred can obtain 50%

Iprodione 2%

Fatty alcohol-polyoxyethylene ether sulfosuccinic acid monoesters disodium 10%

MODIFIED LIGNOSULPHONATE 5%

Xanthans 1%

Bentonite 1%

Glycerine 5%

PVP-K30 1%

Water complements to 100%

Above-mentioned each component is mixed in proportion after grinding and/or high speed shear and obtained seed coat agent.

Can obtain+50% iprodione microcapsule suspension-suspending agent of embodiment 12 5,%00

ATLOX ^TM4913 4%

Citric acid 0.05%

Catalyzer 0.1%

Water 10%

Iprodione 50%

PAPI ^TM 1.35%

SOLVESSO ^TM200 5%

ATLOX ^TM4913 16%

Dispersant LFH 0.3%

Defoamer 0.16%

Urea 4.4%

Hundred can obtain 5%

Water complements to 100%

By PAPI ^tM, hundred can obtain, SOLVESSO ^tM200 oil phases that form add containing ATLOX ^tMin 4913 the aqueous solution, form emulsion.Then heat and be incubated 50 ^ounder C, add catalyst reaction 2 hours.After cooling, obtain hundred available microcapsuless.

ATLOX ^tM4913, dispersant LFH, defoamer, urea, iprodione and water mix in proportion, after grinding and/or high speed shear, obtain suspending agent.

By hundred can obtaining in the suspending agent that microcapsules adds iprodione of obtaining, stir and obtain 5,%00 can obtain+50% iprodione microcapsule suspension-suspending agent.

Can obtain+50% iprodione suspension emulsion of embodiment 13 1,%00

Hundred can obtain 1%

Iprodione 50%

SOLVESSO ^TM200 10%

Ethoxylated castor oil 4%

Fatty alcohol-polyoxyethylene ether sulfosuccinic acid monoesters disodium 5%

MODIFIED LIGNOSULPHONATE 5%

Xanthans 1%

Bentonite 1%

Glycerine 5%

Water complements to 100%

Can be dissolved in SOLVESSO by hundred ^tMin 200, add ethoxylated castor oil, obtain hundred available oil phases;

Fatty alcohol-polyoxyethylene ether sulfosuccinic acid monoesters disodium, MODIFIED LIGNOSULPHONATE, iprodione and water are mixed in proportion, after grinding and/or high speed shear, obtain the suspending agent of iprodione.

By joining the aqueous suspension agent containing iprodione containing hundred available oil phases, obtain suspension emulsion.

Can obtain+0.5% iprodione water dispersible granules of embodiment 14 50,%00

Hundred can obtain 50%

Iprodione 0.5%

MODIFIED LIGNOSULPHONATE 5%

Lauryl sodium sulfate 5%

Urea 5%

Kaolin complements to 100%

By hundred can obtaining, iprodione, dispersant, wetting agent, disintegrant and filler mix in the ratio of formula, becomes wetting powder through air-flow crushing; Add again a certain amount of water to mix extruding and make material.After drying screening, obtain 50,%00 can obtain+0.5% iprodione water dispersible granules.

Can obtain+5% iprodione water dispersible granules of embodiment 15 5,%00

Hundred can obtain 5%

Iprodione 5%

MODIFIED LIGNOSULPHONATE 5%

Lauryl sodium sulfate 5%

Urea 5%

Kaolin complements to 100%

By hundred can obtaining, iprodione, dispersant, wetting agent, disintegrant and filler mix in the ratio of formula, becomes wetting powder through air-flow crushing; Add again a certain amount of water to mix extruding and make material.After drying screening, obtain 5,%00 can obtain+5% iprodione water dispersible granules.

Can obtain+30% iprodione wettable powder of embodiment 16 5,%00

Hundred can obtain 5%

Iprodione 30%

Lauryl sodium sulfate 1%

Sodium lignin sulfonate 1%

Kaolin complements to 100%

Said components is mixed in proportion, and grind, pulverize, be prepared into wetting powder.

Can obtain+50% iprodione bond of embodiment 17 50,%00

Hundred can obtain 50%

Iprodione 50%

Proportioning in above embodiment is weight per distribution ratio.

Biological test example

The combination of active principles of different agricultural chemicals is made to agricultural chemicals, is effectively and the efficiently mode of one of the upper resistance germ of current development novel pesticide and control agricultural.The agricultural chemicals of different cultivars shows three kinds of type of action: summation action, synergistic effect and antagonism after mixing conventionally.But be specially which kind of effect, unpredictable, only have by great many of experiments and just can know.Compound synergic is well filled a prescription, and owing to obviously having improved actual control efficiency, has reduced the usage amount of agricultural chemicals, thereby has delayed widely the drug-fast generation speed of germ, is the important means of integrated control disease.

The present invention can carry out effect analysis by the abundant method of Sun Yun, Colby formula with the combination of iprodione to hundred, finds that the bactericidal composition obtaining has gain effect, and is not only the simple addition of two kinds of medicaments in certain ratio range.

Toxicity Determination

Test 1: to the toxicity test of graw mold of tomato

Adopt spore germination test method(s).Choose the tomato seedling that growing way is consistent, each processing selects 3 basins for examination tomato seedling, under 50PSI pressure, spray with potter spray tower, and the about 5mL of every basin, each medicament arranges 12 concentration gradients.After chemicals treatment, 24h connects bacterium, the graw mold of tomato leaf that picks up from field is evenly shaken off to conidium above tomato seedling and inoculate, and then tomato seedling is put into greenhouse and cultivates.After 7d, investigate disease index according to the morbidity grade scale complete stool of graw mold of tomato, and calculate control efficiency, then calculate concentration EC in inhibition by method of least squares ₅₀, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun.

Computational methods:

Actual measurement toxicity index (ATI)=(standard medicament EC ₅₀/ reagent agent EC ₅₀) * 100

The percentage composition of B in percentage composition+B medicament toxicity index * mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture

Co-toxicity coefficient (CTC)=[theoretical toxicity index of mixture actual measurement toxicity index (ATI)/mixture (TTI) * 100

CTC<80 is antagonism, and 80≤CTC≤120 are summation action, and CTC > 120 is synergistic effect,

Table 1: to the virulence test result of graw mold of tomato

As known from Table 1, hundred can be with the combination control graw mold of tomato of iprodione in the scope of proportioning 100:1-1:100 time, and co-toxicity coefficient is all greater than 120, illustrates that both all show as gain effect at the mixture within the scope of this.

Test 2: the toxicity test of powdery mildew of cucumber

Adopt spore germination test method(s): choose the cucumber seedling that growing way is consistent, each processing selects 3 basins for examination leaf seedlings, under 50PSI pressure, spray with potter spray tower, the about 5mL of every basin, each medicament arranges 12 concentration gradients.After chemicals treatment, 24h connects bacterium, the powdery mildew of cucumber leaf that picks up from field is evenly shaken off to conidium above cucumber seedling and inoculate, and then cucumber seedling is put into greenhouse and cultivates.After 7d, investigate disease index according to the morbidity grade scale complete stool of powdery mildew of cucumber, and calculate control efficiency, then calculate concentration EC in inhibition by method of least squares ₅₀, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun.

Table 2: to the virulence test result of powdery mildew of cucumber

As known from Table 2, hundred can prevent and treat powdery mildew of cucumber in the scope of proportioning 100:1-1:100 time with the combination of iprodione, and co-toxicity coefficient is all greater than 120, illustrates that both all show as gain effect at the mixture within the scope of this.

Test 3: the toxicity test of sclerotinia rot of colza

Adopt spore germination test method(s): choose the Brassica campestris L seedling that growing way is consistent, each processing selects 3 basins for examination seedling, under 50PSI pressure, spray with potter spray tower, the about 5mL of every basin, each medicament arranges 12 concentration gradients.After chemicals treatment, 24h connects bacterium, the sclerotinia rot of colza leaf that picks up from field is evenly shaken off to conidium above Brassica campestris L seedling and inoculate, and then Brassica campestris L seedling is put into greenhouse and cultivates.After 7d, investigate disease index according to the morbidity grade scale complete stool of sclerotinia rot of colza, and calculate control efficiency, then calculate concentration EC in inhibition by method of least squares ₅₀, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun.

Table 3: to the virulence test result of sclerotinia rot of colza

As known from Table 3, hundred can be with the combination control sclerotinia rot of colza of iprodione in the scope of proportioning 100:1-1:100 time, and co-toxicity coefficient is all greater than 120, illustrates that both all show as gain effect at the mixture within the scope of this.

Test 4: the toxicity test of Penicillium italicum

Adopt and suppress mycelial growth rate method: experiment target is designated as Penicillium italicum, test adopts co-toxicity coefficient method to evaluate the co-toxicity effect of complex preparation.

Can use respectively acetone solution with iprodione by hundred, be mixed with again the liquid of series concentration with 0.1% Tween-80 aqueous solution dilution, in superclean bench, draw respectively the conical flask of 6mL to sterilizing, add potato dextrose agar (PDA) 54mL of 50 DEG C of left and right, after shaking up, pour the plate of 4 diameter 9cm into, make the toxic medium of 4 respective concentration; Use the same method and can make toxic medium with iprodione series concentration compound solution by hundred of different proportionings.To cultivate the Penicillium italicum bacterium of 2 days, break into bacterium piece at colony edge with the card punch of diameter 5mm, with transfer needle, bacterium piece is moved to the toxic PDA medium central authorities that are mixed with in advance, be then placed in 25 DEG C of incubators and cultivate, every processing repetition 4 times.After 3 days, adopt right-angled intersection method to measure each processing colony diameter cm with slide calliper rule, obtain correction inhibition percentage.Two diameters are surveyed in each bacterium colony right-angled intersection, represent bacterium colony size with its mean.Then obtain colony growth inhibiting rate by following formula:

Then calculate concentration EC in inhibition by method of least squares ₅₀, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun.

Table 4: to the virulence test result of Penicillium italicum

As known from Table 4, hundred can be with the combination control Penicillium italicum of iprodione in the scope of proportioning 100:1-1:100 time, and co-toxicity coefficient is all greater than 120, illustrates that both all show as gain effect at the mixture within the scope of this.

Biological test example

In the time that the effect of active compound combinations exceedes the summation of the effect in the time that each reactive compound is used separately, there is synergistic function.The predictive role of the particular combinations of two kinds of reactive compounds can use so-called " Colby formula " (referring to S.R. Colby, " Calculating Synergistic and Antagonistic Responses of Herbicide Combinations ", Weeds 1967,15,20-22) calculate as follows: if

X is the activity in the time using consumption for mg/ha or the concentration reactive compound A that is mppm;

Y is the activity in the time using consumption for ng/ha or the concentration reactive compound B that is nppm, is expressed as the percentage that accounts for untreated control;

E is the activity in the time using consumption for m and n g/ha or the concentration reactive compound A that is m and n ppm and B,

So

If the activity of actual observation (O) is greater than expection active (E), so said composition super add and, there is synergistic effect.

Following biological test example is in order to illustrate the present invention.But the present invention is not limited to these embodiment.

Test 1 powdery mildew of cucumber test

Solvent: the N-methyl-pyrrolidones of 7 parts of weight

Emulsifier: the alkylaryl polyglycol ether of 2 parts of weight

Suitable in order to make, 1 part of reactive compound or the combination of reactive compound and solvent of above-mentioned amount and emulsifier is by weight mixed, and be diluted with water to desired concn.

In order to measure activity, the active agent preparations spraying by seedling with described using dosage.Liquid layer to be painted dusts seedling after becoming dry with the spore of cucumber powdery mildew fungus.Then test plant is placed in to temperature approximately 20 ^oin the greenhouse of C, air humidity approximately 80% to promote the growth of white powder scab.Inoculate and carry out effect assessment after 7 days, 0% refer to effect with contrast quite, 100% does not refer to and observes and infect.Reactive compound, using dosage and result of the test are listed in the following table:

Result of the test shows, observes activity and exceedes the activity that use " Colby " formula calculates.

Test 2 ash arrhizus bacteria tests (strawberry)

Solvent: the N-methyl-pyrrolidones of 7 parts of weight

Emulsifier: the alkylaryl polyglycol ether of 2 parts of weight

In order to make suitable reactive compound, 1 part of reactive compound or the combination of reactive compound and solvent of above-mentioned amount and emulsifier is by weight mixed, and be diluted with water to desired concn.

In order to test therapeutic activity, seedling is dusted with Botrytis cinerea bacterium spore.Inoculate after 48 hours the active agent preparations spraying by Strawberry seedlings with described using dosage.Then test plant is placed in temperature approximately 20 ^oin the greenhouse of C, air humidity approximately 80% to promote the growth of grey mold scab.Inoculate and carry out effect assessment after 7 days, 0% refer to effect with contrast quite, 100% does not refer to and observes and infect.Reactive compound, using dosage and result of the test are listed in the following table:

Result of the test shows, observes activity and exceedes the activity that use " Colby " formula calculates.

Test 3 stalk break test (rape)/seed treatment

Reactive compound is used with dry seeds seed dressing.Said preparation can be by mixing the reactive compound of independent reactive compound or combination to prepare with levigate mineral matter, and obtaining can be at the equally distributed finely powdered mixt of the surface of the seed.

When seed seed dressing, shake 3 minutes in the glass flask of sealing infecting seed and seed seed dressing.Rape seed is seeded in 1 centimetre of dark standard soil, in temperature approximately 18 ^oc, cultivates in the greenhouse of air humidity approximately 95%, and seeds cultivation case is illumination in 15 hours day by day often.

Sow after 3 weeks, evaluate the illness of Brassica campestris L seedling, 0% refer to effect with contrast suitable, 100% does not refer to and observes and infect.Reactive compound, using dosage and result of the test are listed in the following table:

Result of the test shows, observes activity and exceedes the activity that use " Colby " formula calculates.

Test 4: to the antiseptic effect of storage oranges and tangerines

Process in oranges and tangerines and pluck tangerine fruit picking time, reject sick fruit and wound fruit, soak really 1min with processing liquid.Contrast clear water dipping.Each processing after soaking fruit dried, and packs surrounding next day into and be lined with the plastic box of newspaper, normal temperature laboratory storage.Process and check decayed fruit number and reject decayed fruit for latter 45 days, weigh up fruit weight simultaneously.And calculate preventive effect.

Result of the test shows, observes preventive effect and exceedes the preventive effect that use " Colby " formula calculates.

Claims (16)

1. a bactericidal composition, is characterized in that: comprise hundred can with iprodione active component, described hundred can be 100:1-1:100 with the weight ratio of iprodione.

2. composition according to claim 1, is characterized in that: described hundred can be 50:1-1:50 with the weight ratio of iprodione.

3. composition according to claim 1, is characterized in that: described hundred can be 25:1-1:25 with the weight ratio of iprodione.

4. composition according to claim 1, is characterized in that: described hundred can be 10:1-1:10 with the weight ratio of iprodione.

5. composition according to claim 1, is characterized in that: comprise the active component described in claim 1, filler and/or surfactant.

6. composition according to claim 1, is characterized in that: hundred can account for the content of iprodione active component the 5%-90% of composition.

7. composition according to claim 1, is characterized in that: hundred can account for the content of iprodione active component the 10%-80% of composition.

8. composition according to claim 1, is characterized in that: hundred can account for the content of iprodione active component the 20%-60% of composition.

9. composition according to claim 1, it is characterized in that, the formulation of described composition is wetting powder, missible oil, suspending agent, suspension emulsion, microcapsules, seed coat agent, microemulsion, aqueous emulsion, water dispersible granules, foaming agent, paste, aerosol, ultra low volume spraying liquid.

10. the purposes of the composition described in claim 1 for preventing and treating fungi.

The purposes of 11. compositions claimed in claim 10 for preventing and treating fungi on Cereal, fruit, vegetables, cotton, fruit tree, ornamental plants.

Prevent and kill off the method for plant disease for 12. 1 kinds, bactericidal composition claimed in claim 1 is applied to plant, plant propagation material and the organ growing subsequently, environment.

13. methods according to claim 12, is characterized in that: the propagating materials of the plant needing protection by compositions-treated claimed in claim 1.

14. methods according to claim 12, is characterized in that: hundred of claim 1 can be used simultaneously or use respectively or sequential application with iprodione compound.

The plant propagation material of 15. compositions-treated claimed in claim 1.

16. compositions claimed in claim 1 are for preventing and treating the purposes of fruit vegetables storing period disease.