KR101976853B1 - 폴리펩티드의 정제 방법 - Google Patents

폴리펩티드의 정제 방법 Download PDF

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KR101976853B1
KR101976853B1 KR1020127033600A KR20127033600A KR101976853B1 KR 101976853 B1 KR101976853 B1 KR 101976853B1 KR 1020127033600 A KR1020127033600 A KR 1020127033600A KR 20127033600 A KR20127033600 A KR 20127033600A KR 101976853 B1 KR101976853 B1 KR 101976853B1
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antibody
polypeptide
cation exchange
buffer
loading
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KR20130086544A (ko
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후이 에프. 리우
브라이언 데이빗 켈리
디아나 이. 마이어스
베쓰 멕쿠이
크리스타 마리 페티
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제넨테크, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020127033600A 2010-05-25 2011-05-25 폴리펩티드의 정제 방법 Active KR101976853B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US34814310P 2010-05-25 2010-05-25
US61/348,143 2010-05-25
PCT/US2011/037977 WO2011150110A1 (en) 2010-05-25 2011-05-25 Methods of purifying polypeptides

Publications (2)

Publication Number Publication Date
KR20130086544A KR20130086544A (ko) 2013-08-02
KR101976853B1 true KR101976853B1 (ko) 2019-05-09

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US (5) US20130079272A1 (https=)
EP (3) EP4492053A3 (https=)
JP (3) JP5934190B2 (https=)
KR (1) KR101976853B1 (https=)
CN (2) CN107365346A (https=)
AU (1) AU2011258254B2 (https=)
CA (2) CA3209878A1 (https=)
ES (2) ES2637613T5 (https=)
HK (1) HK1246328A1 (https=)
HR (2) HRP20241463T1 (https=)
HU (1) HUE068857T2 (https=)
IL (2) IL223029A0 (https=)
MX (2) MX344248B (https=)
MY (1) MY161534A (https=)
NZ (1) NZ603648A (https=)
PL (2) PL3299380T3 (https=)
RU (1) RU2594163C2 (https=)
SG (2) SG185737A1 (https=)
SI (1) SI2575847T2 (https=)
WO (1) WO2011150110A1 (https=)
ZA (1) ZA201208673B (https=)

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KR101976853B1 (ko) 2010-05-25 2019-05-09 제넨테크, 인크. 폴리펩티드의 정제 방법
PT2768857T (pt) * 2011-10-19 2020-01-27 Novimmune Sa Métodos para purificar anticorpos
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CN103880947B (zh) * 2012-12-21 2016-01-13 武汉禾元生物科技股份有限公司 一种分离纯化高纯度重组人血清白蛋白的层析方法
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CN109765307B (zh) * 2018-12-24 2021-06-29 山东省科学院海洋仪器仪表研究所 一种水体中六氯环己烷的测量方法
CN111366547A (zh) * 2018-12-26 2020-07-03 贵州中烟工业有限责任公司 一种用连续流动法测定烟草或烟草制品中α-氨基氮的检测方法
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Publication number Publication date
JP2018087192A (ja) 2018-06-07
HK1246328A1 (zh) 2018-09-07
RU2594163C2 (ru) 2016-08-10
JP2016190851A (ja) 2016-11-10
PL2575847T3 (pl) 2017-10-31
EP3299380B1 (en) 2024-08-28
CN107365346A (zh) 2017-11-21
KR20130086544A (ko) 2013-08-02
EP2575847B2 (en) 2022-04-27
AU2011258254A1 (en) 2013-01-10
EP4492053A3 (en) 2025-03-19
JP6272941B2 (ja) 2018-01-31
SI2575847T2 (sl) 2022-08-31
SG10201710439UA (en) 2018-01-30
CA2799915C (en) 2023-09-26
US20220064209A1 (en) 2022-03-03
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