KR101875190B1 - Detergent composition composition comprising tengcha - Google Patents

Abstract

The present invention relates to a detergent composition using Tengcha. More particularly, the composition comprises 0.1-10 wt% of an extract of Tengcha as an active ingredient based on the total weight of the whole composition. According to the present invention, the composition has a cleansing power similar to that of a conventional synthetic detergent, has not only antioxidant and bactericidal activity of the extract of Tengcha, but also has little irritation and does not cause environmental pollution.

Description

{DETERGENT COMPOSITION COMPOSITION COMPRISING TENGCHA}

The present invention relates to a detergent composition, and more particularly, to a detergent composition which is excellent in detergency, and which has skin protection, sterilization and an antimicrobial effect.

Detergents can be classified into domestic and industrial use depending on the application field, and in particular households are classified into kitchen, laundry, and cleaning. In addition, depending on the kind of raw materials used in the detergent, it can be classified into a synthetic detergent and a natural detergent.

As consumers are increasingly seeking well-being, the popularity of natural detergents using natural ingredients is increasing day by day.

Especially, as the number of restaurants and restaurants related to home and food have recently increased, the amount of kitchen detergent used for washing dishes has been rapidly increasing. In addition, as the concept of well-being increases, the demand for natural materials, which clean dishwashing detergents and harmful to the hands of the family, is increasing.

However, the conventional kitchen detergent does not have an antibacterial effect or a sterilizing effect at the same time in addition to the cleaning effect. Known as a dishwashing detergent with antimicrobial effect, a kitchen detergent containing an antibacterial ingredient known as Nonoxynol-9 is manufactured and sold. However, it is toxic to the human body when used with strong irritating odor and is used as a kitchen detergent It was unsuitable to be. Such a cleaning agent can not effectively sterilize harmful bacteria or viruses adhering to dishes through food or the like during the cleaning process.

On the other hand, detergents such as lactose, which is mainly used for sterilization and cleaning, are known to sterilize about 50 kinds of bacteria, but they are not used because of their strong irritating odor and toxicity to the human body when consumed.

On the other hand, clothes that come into direct contact with the skin are closely related to the hygiene and health of the individual in terms of being in contact with the body 24 hours a day. In particular, chemicals such as surfactants, fluorescent whitening agents, and synthetic coloring agents in detergents that can have a detrimental effect on humans cause dermatitis, such as atopy and housewife eczema. In other words, washing with synthetic detergent is like applying chemical contaminants to the skin, so the most important point in selecting a detergent is washing power, but it is more important to find a safe detergent. In particular, children's clothing detergents that directly touch the skin of a baby should be safe, less irritating to the skin, and have good antimicrobial effects. As a result, consumers are increasingly interested in the ingredients and functions of detergents for washing clothing. Recently, as environment-friendly ethical consumption has become a trend in the consumer market, detergents for clothing that minimize environmental pollution have attracted particular attention

Therefore, it is necessary to develop natural detergent which combines not only detergent with low pollution and high functionality but also antibacterial function made by using natural raw material rather than synthetic detergent due to recent seriousness of environmental problems and concern about health, to be.

KR 10-1624951 B1 KR 10-1300632 B1

Accordingly, an object of the present invention is to provide a detergent composition which is excellent in detergency, has an antibacterial and germicidal effect, and has low skin irritation by using a natural substance which is not a chemical synthetic substance.

In order to achieve the above object, the present invention provides a detergent composition using the isoquinolone, which comprises 0.1 to 10% by weight of an isostatic extract as an active ingredient based on the total weight of the whole composition.

The present invention also relates to a cosmetic composition comprising as an active ingredient 0.1 to 5% by weight of an extract of fermented tea extract and 20 to 30% by weight of a surfactant, wherein the surfactant is disodium laureth sulfosuccinate (LES) Surfactants, surfactants, potassium cocoyl glycinate (PCG), and cocobetaine. [0027] The term " surfactant "

The composition further comprises 5 to 15% by weight of organic acid, 0.1 to 0.5% by weight of essential oil, 0.1 to 5% by weight of extract, 0.1 to 5% by weight of an extract of P. forest leaf and sodium hydrogen carbonate in a remaining amount.

The composition can be used in various applications such as kitchen detergents, floor cleaners, toilet cleaners, kitchen disinfectants, bathroom sanitizers, glass cleaners, fruit vegetable cleaners, oil cleansers, grease cleaners, bleach cleaners, And a toilet cleaner.

INDUSTRIAL APPLICABILITY The detergent composition of the present invention has an antibacterial activity and an antibacterial activity similar to those of the conventional synthetic detergents, but also has less irritation to the skin and does not cause environmental pollution.

Hereinafter, the present invention will be described in detail.

The present invention is to provide a detergent composition. The detergent composition includes all kinds of detergent for kitchen, laundry, and cleaning. The detergent composition may be in the form of a liquid or a powder.

The detergent composition according to the present invention is characterized by comprising an isostatic extract in an amount of 0.1 to 10% by weight based on the total weight of the whole composition.

Here, the tengcha (Ampelopsis grossedentata) is a kind of wild stem plant of grape and beetle, which is known as moyeam. Most of the above-mentioned islands grow in Hongsam, Mt. Monsan, 800 ~ 1500m high, and germinate in spring and fade in autumn. Stems and leaves have no hairs. Stem diameter is about 0.2 ~ 0.4cm. The biggest annual output is early summer The next is autumn. This is a rare and rare treasure missing from the main river gem. It contains 17 kinds of amino acids such as leucine, isoserine and methionine and 14 kinds of trace elements such as potassium, calcium, zinc and selenium. Dihydromyricetin (DMY) , anthocyan (OPC), and flavone (Flavone) are very high. More specifically, the above equilibrium has the highest flavonoid content among the currently found plants with a flavon content of 7.5-8.3% (minimum content ≥6%), crude protein content is 12.8-13.8%, and Dihydromyricetin content is about 25% It reaches.

In the present invention, the stem and leaf of such an isola are collected and extracted and used. When the extract of this isola is used, antioxidation and antimicrobial activity are imparted to the detergent composition to improve detergent washing ability, sterilization and antibacterial property , Which protects the skin and relieves the skin irritation caused by the detergent.

In the present invention, the extraction of the equipotential may be performed by hot water extraction, cold extraction, warming extraction, reflux cooling extraction, ultrasonic extraction, or the like. In addition, the extraction is carried out using a solvent, and water, ethanol, or a mixed solvent thereof may be used as the solvent.

More specifically, the extraction is carried out by adding a dry weight of 3 to 20 times by weight of a solvent to the dried powder of the stem, the leaves or the mixture of the isomorphs and drying the solution at 20 to 100 ° C, more preferably at an extraction temperature of 40 to 90 ° C For 30 minutes to 5 days, more preferably for 1 to 24 hours. It also includes, if necessary, concentrating, lyophilizing and pulverizing. Here, the lyophilization follows a known method in the field to which this technique belongs, and thus a detailed description thereof will be omitted.

The dry powder refers to a product obtained by picking up the stem and leaves of the isola and drying it through a method such as a hair dryer, shade, hot air drying, etc., and then, It is sufficient if it is crushed to 50 ~ 300mesh as an example. In addition, it may be crushed and dried, followed by shredding.

In the present invention, it is preferable that the extract of the present invention is contained in an amount of 0.1 to 10% by weight based on the total weight of the detergent composition. If the content is too small, the antibacterial and bactericidal properties are not good. It is because.

The detergent composition of the present invention may further comprise a surfactant, which may be disodium laureth sulfosuccinate (LES), an olive surfactant, an apple surfactant, a potassium cocoyl glycine It is preferable to use at least one selected from Potassium Cocoyl Glycinate (PCG) and Coco-betaine. This is because the use of a natural surfactant not only stimulates skin and eyes, but also has good bubble power and fast biodegradability to prevent environmental pollution.

In the present invention, it is preferable that the surfactant is contained in the range of 20 to 30% by weight based on the total weight of the detergent composition. If the content is too small, foam formation is not sufficient, This is because it causes a deterioration in the overall quality of the detergent.

The detergent composition of the present invention may further comprise 0.1 to 5% by weight of an isobaric fermentation extract together with 0.1 to 10% by weight of an equal weight extract and 20 to 30% by weight of a surfactant, This is because it has more excellent antibacterial and germicidal properties.

In this case, the fermentation extract of the present invention is prepared by adding water and saccharide to the above-described extract of the order tea at a weight ratio of 1: 1 to 10: 0.1 to 0.2, and inoculating the fermentation microorganism with the amount of 100,000 to 10,000,000 cfu / L Followed by fermentation at 20 to 40 ° C for 12 to 60 hours. The fermented fermentation broth is centrifuged to separate only the supernatant. The supernatant may be dried and used. When the extract is dried, it is extracted with an extraction solvent of 1 to 20 times its dry weight, and is then immersed at 4 to 30 ° C for 3 to 20 days to extract an active ingredient. The filtrate is concentrated under reduced pressure And lyophilization. The extraction by the extraction solvent may be performed using any extraction solvent conventionally used in the art, but extraction is performed using at least one solvent selected from the group consisting of water and ethanol.

At this time, it is preferable to use Saccharomyces genus as the fermenting microorganism, but Saccharomyces cerevisiae ATCC 18824 is more preferably used. As the sugar, , Oligosaccharides, and the like.

The detergent composition of the present invention may contain 5 to 15% by weight of organic acids, 0.1 to 0.5% by weight of essential oils, 0.1 to 5% by weight of buttercups extract, 0.1 to 5% % And a remaining amount of sodium hydrogencarbonate.

The organic acid is a deodorant component for eliminating odor, and also helps sterilization. As the organic acid, at least one selected from citric acid, malic acid, succinic acid, tartaric acid, sorbic acid, ascorbic acid, lactic acid, acetic acid and clonic acid may be used. The content of the organic acid is in the range of 5 to 15% by weight. When the content of the organic acid is less, the foaming power is lowered. If the content is excessive, the acid causes irritation to the skin.

The essential oil is an aromatic and antimicrobial ingredient that provides a natural fragrance and acts to enhance the deodorizing effect. These essential oils include lemon essential oils, lemongrass essential oils, lime essential oils, orange essential oils, orange sweet essential oils, grapefruit essential oils, lavender essential oils, jasmine essential oils, rosemary essential oils, chamomile essential oils, Essential Oils and Ylang Ylang Essential Oils can be selected and used. The essential oil is used in an amount of 0.1 to 0.5% by weight. If the content of the essential oil is too small, the effect is insignificant, and if it is excessive, the essential oil is not economical.

 When the buttercups extract is further contained, there is an effect that the sterilizing power and the rinsability are further improved. The dried buttercups extract is obtained by hot water extraction, cold extraction extraction, heating extraction, reflux cooling extraction and ultrasonic extraction using water, ethanol or a mixed solvent thereof, in the same manner as the extraction method of the above-mentioned extract.

At this time, the dry powder of the buttercups is dried by a known method in such a range that the useful components of the buttercups are not destroyed, dried by, for example, natural drying in a shade, It is sufficient if it is crushed to such an extent that it can be extracted. In addition, the drying and crushing processes may be carried out in reverse order or repeatedly, if necessary, and may be omitted.

If the content is less than 0.1% by weight, the effect is insignificant. If the content is excessive, the increase in the effect of the extract is insignificant and the stability of the formulation is insufficient. This is because a problem that can not be secured may appear.

When the above-mentioned Allium cepa leaf extract is further contained, the sterilizing power and the rinsability are further improved. In the same manner as in the extraction method of the above-mentioned extract of the order tree, the extract of the Aspergillus oryzae is obtained by hot water extraction, cold extraction, warming extraction, reflux cooling extraction and ultrasonic extraction using water, ethanol or a mixed solvent thereof will be.

At this time, the dried powder of the perennial apricot leaf is dried by a known method within a range that useful components of the perennial apricot leaves are not destroyed, dried by a natural drying method, for example, in a shade field, It is sufficient if the useful components of the leaf are crushed to such an extent that it can be extracted sufficiently. In addition, the drying and crushing processes may be carried out in reverse order or repeatedly, if necessary, and may be omitted.

If the content of the extract is less than 0.1% by weight, the effect is insignificant. When the content is excessively large, the effect of the extract is not greatly increased. There is a problem that stability can not be secured.

Sodium bicarbonate is used as a neutralizing agent. If it is excessive, precipitation may occur, and if used in a small amount, detergency may decrease.

In addition, the detergent composition of the present invention can be manufactured in any form conventionally manufactured in the art, and the use field thereof is not limited. Specifically, the detergent composition for kitchen detergent, floor detergent, toilet detergent, disinfectant for kitchen, A laundry detergent for clothes, a fabric softening agent, and a toilet cleansing agent, for example, a disinfectant, a glass cleaner, a fruit vegetable cleaner, an oil cleanser, a stain remover, a bleaching agent, a household cleaner,

Hereinafter, the present invention will be described in more detail with reference to the following experimental examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example  1: Preparation of equine tea extract

Stems and leaves of Ishikari were collected at a weight ratio of 1: 1, washed thoroughly, and shaken to a moisture content of 15%, which was ground to about 100 mesh. Next, 20 parts by weight of distilled water was added thereto, and the mixture was extracted at 70 to 90 DEG C for 24 hours. The mixture was filtered through a filter paper, concentrated under reduced pressure using a rotary evaporator, and then lyophilized to obtain a powdery extract.

Example  2: Preparation of equine tea extract

Stems and leaves of Ishikari were collected at a weight ratio of 1: 1, washed thoroughly, and shaken to a moisture content of 15%, which was ground to about 100 mesh. Next, 10% by weight of 80% ethanol was added thereto, and the mixture was stirred at 20 캜 for 24 hours for extraction. The mixture was filtered through a filter paper, concentrated under reduced pressure using a rotary evaporator, and then lyophilized to obtain a powdery extract.

Example 3: Preparation of a complex extract

The isochrone extract of Example 1 and the equimolar fermentation extract were mixed at a weight ratio of 1: 0.5.

Water and sugar were added to the extract of Example 1 at a weight ratio of 1: 9: 0.1, then Saccharomyces cerevisiae ATCC 18824 was inoculated at 100,000 to 10,000,000 cfu / L, and cultured at 32 ° C for 24 hours And fermented. After the fermentation, the fermentation broth was centrifuged to remove bacteria, ethanol was added thereto to a final concentration of 70% (v / v), refluxed three times for 5 hours, cooled, and filtered. This was concentrated under reduced pressure and lyophilized.

Example 4: Preparation of complex extract

The isochrone extract of Example 2 and the equimolar fermentation extract were mixed at a weight ratio of 1: 0.5.

The fermented extract was prepared by the same method as in Example 3, except that the extract of Example 2 was used.

Experimental Example  1: Antioxidant effect measurement

Free radical scavenging test and an active oxygen scavenging test were conducted to confirm the antioxidative effect of the extracts of Examples 1 to 6 above.

Free radical scavenging test

Using the fact that the absorbance of stable DPPH exhibits the maximum absorbance at 540 nm, it can be said that as the free radical DPPH is erased by the sample and becomes transparent color from purple, that the absorbance decreases at the above 540 nm wavelength as the free radical scavenging rate is increased And proceeded by the following test method.

First, 1 ml of a 0.1 mM DPPH (2,2-diphenyl-1-picryl-hydrazyl radical, Sigma) solution was diluted with a suitable concentration of methanol, and the mixture was incubated at 37 ° C for 15 minutes. Absorbance was measured at a wavelength of 540 nm using a reader (UVT-06685, Thermo max, USA).

In the free radical scavenging test, 1 ml of DPPH and 1 ml of methanol were added to the control, and 1 ml of methanol and 1 ml of the sample were added to obtain the respective color correction values for the sample and the control.

The free radical scavenging ratio was calculated using the following equation (1), and the results are shown in Table 1 below. In Table 1, SC ₅₀ represents the concentration of the sample required to eliminate 50% of the free radicals. The smaller the value, the higher the antioxidant activity.

(1)

Free radical scavenging rate (%) = 100 - {(absorbance of sample / absorbance of control) x 100}

Reactive oxygen scavenging test

The measurement of the absorbance change of nitroblue tetrazolium (NBT) by reactive oxygen species by the enzyme reaction of xanthine / xanthine oxidase (Sigma) It is possible to know the scavenging ability. 0.1 ml of sodium carbonate (Na ₂ CO ₃ ), 0.1 ml of xanthine (Sigma), 0.1 ml of EDTA (ethylenediamine tetraacetic acid), 0.1 ml of BSA (bovine serum albumin, Sigma), 0.1 ml of NBT and 0.1 ml of a sample were placed, (Type 37600 Mixer, Mini mix, USA). The mixture was incubated at 25 ° C for 10 minutes, 0.1 ml of xanthine oxidase was added, and the mixture was incubated at 25 ° C for 20 minutes. ₂ ) was added to stop the reaction, and the absorbance was measured at a wavelength of 540 nm using a microplate reader (UVT-06685, Thermo max, USA).

The control group in the active oxygen scavenging activity test was prepared by adding the third distilled water instead of the sample and measuring by the same method. Third distilled water was added instead of the xanthine oxidase solution to obtain the respective color correction values for the extracted sample and the control group Respectively.

The active oxygen scavenging rate was calculated numerically using the following equation (2), and the results are shown in Table 1. In Table 1, the IC ₅₀ is a sample concentration required to eliminate 50% of active oxygen, and a smaller value means that the antioxidant activity is stronger.

(2)

(%) = 100 - {(absorbance of sample / absorbance of control) x 100}

Results of Experimental Example 1 division Free radical scavenging rate
SC ₅₀ (mg / ml) Active oxygen scavenging rate
IC ₅₀ (mg / ml) Example 1 0.142 0.081 Example 2 0.131 0.072 Example 3 0.113 0.057 Example 4 0.101 0.053

As can be seen from the above Table 1, the extract of the present invention showed excellent antioxidative effect.

Experimental Example  2: Antimicrobial activity measurement

In order to confirm the antibacterial effect of the extracts of Examples 1 to 4, an antibacterial test was conducted by a paper disk method. Staphylococcus aureus KCTC 6910, Gram-negative bacteria Pseudomonas aeruginosa KCTC 1637, E. coli KCTC 1039, Candida albicans KCTC 7965 and yeast were used as test strains. A total of five strains of Aspergillus niger KCTC 6910 were used.

One liter of each strain cultivated on a plate medium was inoculated into 10 ml of the liquid medium and incubated for 6 hours. After incubation for 6 hours, the bacterial cells per plate medium 10 < ⁷ > CFU / ml and homogenously smoothed with a sterilized smear bar. Then, the sterilized paper disk (6 mm, Satorius, Germany) was placed on a solid plate medium, and the sample dissolved in the solvent was absorbed to be 0.05 ml / disk and cultured. Staphylococcus aureus, Phseudomonas aeruginosa, Escherichia coli were cultured at 37 ° C, Candida yeasts (Chandida albicans) and Aspergillus niger (fungi) were cultured at 27 ° C for 24 hours and 120 hours respectively And the diameter of the transparent zone around the disk was measured. As a positive control, methylparaben, which is known to have a strong antibacterial activity, was used, and the results are shown in Table 2. At this time, the transparent zone around the disc is an indicator of the degree of inhibition of growth of the strain, and the larger the diameter, the higher the antibacterial effect against the strain.

Experimental Example 2 Results Strain Diameter (mm) Staphylococcus
Example 1 13 Example 2 14 Example 3 17 Example 4 18 Methyl paraben 11 P. aeruginosa
Example 1 13 Example 2 15 Example 3 15 Example 4 16 Methyl paraben 12 Escherichia coli
Example 1 19 Example 2 21 Example 3 22 Example 4 24 Methyl paraben 18 Candida yeast
Example 1 14 Example 2 15 Example 3 16 Example 4 17 Methyl paraben 13 Black germ
Example 1 26 Example 2 27 Example 3 29 Example 4 30 Methyl paraben 24

As can be seen from the above Table 2, the extract according to the present invention showed stronger antibacterial activity than methylparaben, which is known as a strong antibacterial agent.

Manufacturing example  1 to 4: Preparation of detergent composition

The detergent compositions containing Examples 1-4 of the present invention were prepared in a conventional manner at the following proportions.

Production Example 1 was prepared by adding 20 g of the extract of Example 1, 50 g of LES, 20 g of citric acid and 1 g of lemon essential oil to 109 g of sodium hydrogencarbonate and stirring.

Production Example 2 was carried out in the same manner as in Production Example 1 except that 20 g of sodium hydrogencarbonate was replaced with 10 g of dried fruit extract and 10 g of the forest leaf extract.

Production Examples 3 and 4 were carried out in the same manner as Production Examples 1 and 2 except that the extract of Example 3 was used instead of the extract of Example 1.

The watercress extract was obtained by adding 15 parts by weight of water to millet and heating it at 80 to 100 ° C for 20 hours, filtering it, concentrating it under reduced pressure at 50 ° C using a reduced pressure concentrator, and lyophilizing it to obtain a powdery extract .

The leaf of Leucospermum japonica L. was shaded with a moisture content of less than 10%, and about 15 times by weight of water was added thereto, followed by hot water extraction at 80 to 90 ° C for 15 hours. The mixture was filtered, concentrated under reduced pressure at 50 ° C using a vacuum concentrator, and lyophilized to obtain a powdery extract.

Comparative Manufacturing Example  One

Was prepared in the same manner as in Preparation Example 1, except that sodium hydrogencarbonate was used instead of the extract of Example 1.

Experimental Example  3: Foam amount , Cleaning power, skin irritation, Rinseability  evaluation

In Experimental Example 3, a commercial synthetic kitchen detergent was used as a control group.

Foam amount

For the measurement of the amount of foam, the detergent compositions prepared in Preparation Examples 1 to 4 and Comparative Preparation Example 1 were diluted with water to a concentration of 0.1% by weight, and 10 ml was added to a 100 ml cylinder. After shaking 50 times , And a method of measuring the height of the generated foam amount is selected. The height of the foam was indicated by the volume value indicated in the cylinder.

Detergency evaluation

For the evaluation of the detergency, the specimen having the rolling oil having a size of 10 x 10 cm2 was immersed in the detergent composition prepared in the above Production Examples 1 to 4 and Comparative Production Example 1 for 10 seconds to degrease the rolling oil remaining on the surface of the specimen. The degreased steel sheet was washed with distilled water, dried with a drier, cut into a size of 10 × 2.5 cm 2, and the surface residual amount of the specimen was measured five times with RC-212 carbon analyzer.

Evaluation of skin irritation

The detergent composition prepared in Preparation Examples 1 to 4 and Comparative Preparation Example 1 was patched for 5 hours on the tops of 10 healthy males and females, and skin irritation of the patch area was evaluated. All skin irritation test values were compared with the skin irritation level by water, and the skin irritation by water was set as a criterion having a 'very good' value.

The symbol 는 indicates no abnormality, ◯ indicates a slightly reddish phenomenon than other skin, △ indicates a slight allergic symptom, and X indicates a symptom unusual to the skin.

Rinseability  evaluation

The rinsability evaluation was performed by measuring the amount of water used until a certain amount of the dishwasher was washed by burying a certain amount of the detergent composition prepared in Production Examples 1 to 4 and Comparative Production Example 1 in a sponge, Respectively.

The judgment code x is not good,? Means normal, and? Means very good.

The results are shown in Table 3 below.

Experimental Example 3 Results. division Foam volume (ml) Cleaning power Skin irritation Rinseability Production Example 1 90 90.1 ● ○ Production Example 2 91 91.2 ● ○ Production Example 3 90 93.3 ● ○ Production Example 4 92 94.5 ● ○ Comparative Preparation Example 1 82 56.1 ○ ○ Control group 86 90.5 △ △

As can be seen from the above Table 3, Examples 1 and 2 according to the present invention were found to have good foamability and detergency as well as good rinseability and less skin irritation as compared with Comparative Example 2, which is a synthetic detergent .

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the scope of the present invention is not limited to the disclosed exemplary embodiments. It will also be appreciated that many modifications and variations will be apparent to those skilled in the art without departing from the scope of the present invention.

Claims (4)

With respect to the total composition weight,
0.1 to 5% by weight of an extract of the order tea, 0.1 to 5% by weight of an isobaric acid fermentation extract, 20 to 30% by weight of a surfactant, 5 to 15% by weight of an organic acid, 0.1 to 0.5% 0.1 to 5% by weight of leaf extract, and the balance sodium hydrogen carbonate as an active ingredient,
The fermented extract of the present invention is prepared by mixing the extract of the order tea, water and sugar in a weight ratio of 1: 1 to 10: 0.1-0.2, and inoculating the fermenting microorganism in an amount of 100,000 ~ 10,000,000 cfu / Fermented for 60 hours, centrifuged to separate only the supernatant,
The fermenting microorganism is Saccharomyces cerevisiae ATCC 18824,
The surfactant may be selected from Disodium Laureth sulfosuccinate (LES), an olive surfactant, an Apple surfactant, Potassium Cocoyl Glycinate (PCG), and Cocobetaine Wherein the detergent composition is at least one kind selected from the group consisting of a detergent composition and a detergent composition.
delete delete The method according to claim 1,
The composition may comprise,
Kitchen detergent, floor cleaner, toilet detergent, kitchen disinfectant, bathroom disinfectant, glass cleaner, fruit vegetable cleaner, oil cleanser, stain remover, bleach, household cleaner, laundry detergent for clothes, fabric softener, Wherein the detergent composition is at least one kind of detergent composition.