KR102095348B1 - Detergent composition comprising Katsura tree bark extract or Artemisia Capillaris extract - Google Patents

Abstract

The present invention relates to a detergent composition and, more specifically, to a detergent composition having an antibacterial and antifungal activity by including cercidiphyllum japonicum bark extract or artemisia capillaris extract as an active ingredient. According to the present invention, the detergent composition comprising cercidiphyllum japonicum bark extract, artemisia capillaris extract, or a mixture thereof shows a strong antibacterial activity against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa which are major pathogenic bacteria. Specifically, the detergent composition produced by adding yeast extract to the extract not only has an antifungal activity against Candida albicans and Aspergillus brasiliensis but also can be useful as an environmentally friendly functional detergent composition by having excellent biodegradability. With the cercidiphyllum japonicum bark extract, the artemisia capillaris extract, and the yeast extract as natural ingredients, the detergent composition of the present invention has advantages of being safe to the human body even for long time.

Description

Detergent composition comprising Katsura tree bark extract or Artemisia Capillaris extract

The present invention relates to a detergent composition, and more particularly, to a detergent composition having antibacterial and antifungal activity, including cassia bark extract or phosphorus wormwood extract as an active ingredient.

Detergent is a mixture used to remove contaminants attached to the surface of objects by complex physical and chemical effects such as adsorption, wetting, deterioration of interfacial tension, emulsification, solubilization, dispersion, etc. As a domestic consumption industry with a large burden of transportation costs and a small amount of imports and exports, the domestic detergent industry has rapidly grown to become a staple in everyday life as well as to improve the living standards of the people.

Generally, detergents can be divided into natural detergents and synthetic detergents. Natural detergents such as soap have been proven to be harmless to humans and livestock by their long-standing history, but synthetic detergents are a problem for toxicity and environmental pollution. With the recent economic growth, the necessity of developing natural products to replace synthetic products is increasing due to the social trend of improving people's living standards and prioritizing healthy living and consumption. As such natural substances, herbal medicines, seaweeds, or edible plants have long been used in the private sector and have been proven safe, and are attracting attention as they contain various functional substances such as antibacterial, antioxidant, and immune.

Particularly, among the detergents, it is considered that the detergent for the kitchen has an excellent antibacterial effect as well as an excellent washing power, but the conventional detergent for the kitchen does not have an antibacterial effect and a sterilizing effect at the same time in addition to the washing effect. Known as a kitchen cleaner having an antibacterial effect, kitchen detergents including an antibacterial component known as Nonoxynol-9 are manufactured and marketed, but such detergents are also harmful bacteria attached to dishes through food, etc. Viruses cannot be effectively sterilized in the cleaning process.

On the other hand, detergents such as Lax, which are mainly used for sterilization and cleaning, are known to be able to sterilize about 50 kinds of bacteria, but are not suitable for use as kitchen detergents due to strong irritating odor and toxicity to the human body when ingested. Moreover, in order to increase its effectiveness in the cleaning process using synthetic detergents, a lot of bubbles, which are the main culprit of pollution, have to be consumed, and considerable labor, time, and water, such as whipping, rubbing, and tapping, are consumed.

Therefore, in sanitation at home or restaurants, dishes are often boiled and sterilized, or dishes are sterilized using a separate ultraviolet disinfection device. As described above, the operation for separate sterilization after washing with detergent is cumbersome, and if neglected, there is a risk of food poisoning or odor caused by bacteria. In addition, when the user's skin is directly exposed to the detergent, irritation is applied to the skin and there is a fear that the skin is damaged when exposed to the skin for a long time.

Recently, due to the increasing severity of environmental problems and increased interest in health, there is an increasing need to develop low-pollution, high-functional detergents made from natural ingredients rather than synthetic detergents. In line with this trend, the use of detergents using natural antibacterial materials derived from plants is also increasing in recent years. However, these natural materials have not only low cleaning power compared to general synthetic detergents, but also have insufficient antibacterial effects.

Accordingly, the present inventors have researched to find a material that can be applied to detergents as a natural material having excellent antibacterial properties and strong cleaning power. As a result, the detergent composition containing cassia bark extract and phosphorus wormwood extract as active ingredients is strong against major pathogenic bacteria. It was confirmed that it exhibits antibacterial activity, especially in the case of a detergent composition prepared by adding a yeast extract in addition to the above-mentioned materials, it also shows antibacterial activity against pathogenic bacteria, Staphylococcus aureus, E. coli, Pseudomonas aeruginosa, and also fungi such as yeast and fungi The present invention was completed by confirming that it shows strong antibacterial activity.

Korean Registered Patent No. 10-0486861 Korean Patent Publication No. 10-1999-0026389

Accordingly, an object of the present invention is to provide a detergent composition having human body safety and eco-friendliness by using natural materials, and having excellent antibacterial activity against major pathogenic bacteria.

In order to achieve the object of the present invention as described above,

The present invention provides a detergent composition comprising a cassia bark extract, sagebrush extract or a mixture thereof.

In one embodiment of the present invention, the composition may further include a yeast extract.

In one embodiment of the present invention, the extract may be included in 0.1 to 10 parts by weight based on 100 parts by weight of the total composition.

In one embodiment of the invention, The composition may further include one or more selected from the group consisting of purified water, foaming agent, neutralizing agent, water softening agent, flavoring agent and antioxidant.

In one embodiment of the present invention, the composition may have a liquid formulation.

In one embodiment of the present invention, the composition may have antibacterial and antifungal activity.

In one embodiment of the present invention, the composition may have antibacterial activity against Staphylococcus aureus, E. coli and Pseudomonas aeruginosa.

In one embodiment of the present invention, the composition may have antifungal activity against Candida albicans and Aspergillus brasiliensis.

Detergent compositions comprising a cassia bark extract, phosphorus wormwood extract or a mixture thereof according to the present invention show strong antibacterial activity against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, which are major pathogenic bacteria. Particularly, in the case of a detergent composition prepared by adding a yeast extract to the extract, it not only has antifungal activity against Candida albicans and Aspergillus brasiliensis at the same time, but also has excellent biodegradability, so it can be usefully used as an eco-friendly functional detergent composition You can. In particular, the cassia bark extract, the extract of sagebrush, and the yeast extract are natural materials, and the detergent composition of the present invention has the advantage of being safe for the human body even for long-term use.

1 is a photograph showing the detergent composition of the present invention.

The present invention is characterized by providing a detergent composition comprising a cassia bark extract, a sagebrush extract, or a mixture thereof.

The ' Cyrus tree' referred to in this specification is a deciduous broad-leaved tree of the dicotyledonous family, Cassia family, which is scientifically named It is Japonicum S. et Z, and the foliage is beautiful in autumn and the scent is good during flowering. Cassia is 7m in height and 1.3m in diameter. It grows upright and has many large branches with small branches. The leaves are 4-8cm long and the butterfly is 3-7cm long, with a slightly dull end. The front side is green, the back side is white, and there are 5 to 7 palm-shaped veins, and the edges have dull teeth.

As used herein, 'Inginseng ( Artemisiacapillaris Thunb.)' Is a perennial herb that is used as an edible in Korea and is also one of the medicinal plants used in the treatment of various inflammatory diseases. Recently, research on the physiological activity of jinsugum has been actively conducted, and it has been shown that the inhibitory action of inflammation (Jang et al., 2005), the inhibitory effect of hepatitis B (Zhao et al., 2014), and the lipolytic action (Jang et al. , 2014), anti-obesity effect (Hong et al., 2009), anti-cancer effect (Choi et al., 2013), antioxidant effect (Hong and Lee, 2009), platelet aggregation inhibitory effect (Wu et al., 2001), etc. Various effects have been identified.

The present inventor researched to find a material that can be applied to detergents for various uses as a natural material having excellent antibacterial properties and strong cleaning power, and as a result, a detergent composition comprising a cassia bark extract and a sagebrush extract as an active ingredient is a major pathogenic bacteria. It was confirmed to show strong antibacterial activity.

Therefore, the composition of the present invention comprising a cassia bark extract, sagebrush extract, or a mixture thereof is a kitchen detergent, a floor detergent, a toilet detergent, a kitchen disinfectant, a bathroom disinfectant, a glass cleaner, a fruit vegetable cleaner, a degreaser, a steamer It can be usefully used as a detergent composition such as dirt remover, bleach, household cleaner, laundry detergent for clothing, fabric softener, toilet cleaner, shampoo, conditioner, body cleanser, and toothpaste.

The extract according to the present invention can be obtained by extraction and separation from nature using extraction and separation methods known in the art, and the 'extract' defined in the present invention is a cassia bark using an appropriate solvent or It is extracted from phosphorus wormwood, and includes, for example, the cassia bark or crude extract of phosphorus wormwood, a polar solvent soluble extract or a non-polar solvent soluble extract.

As a suitable solvent for extracting the extract from the cassia bark or sagebrush, any pharmaceutically acceptable organic solvent may be used, and water or an organic solvent may be used, but is not limited thereto. Alcohols having 1 to 4 carbon atoms, including purified water (water), methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, Various solvents such as benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane may be used alone or in combination. Preferably, water or ethanol is used.

As an extraction method, any one of methods such as hot water extraction, cold immersion extraction, reflux cooling extraction, solvent extraction, water vapor distillation, ultrasonic extraction, elution, and compression can be used. In addition, the desired extract may be further subjected to a conventional fractionation process, or may be purified using a conventional purification method. There is no limitation in the method for producing the cassia bark extract or jinsukmu extract of the present invention, and any known method may be used.

For example, the cassia bark or phosphorus wormwood contained in the composition of the present invention is prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying of the primary extract extracted by hot water extraction or solvent extraction. can do. In addition, the primary extract is further purified using a variety of chromatography such as silica gel column chromatography (silica gel column chromatography), thin layer chromatography, high performance liquid chromatography (high performance liquid chromatography) You can also get

Therefore, in the present invention, the cassia bark or the ginseng wormwood is a concept including all of the extracts, fractions and refinements, their diluents, concentrates or dried products obtained at each stage of extraction, fractionation or purification.

In the present invention, the cassia bark or the ginseng wormwood can be extracted by putting them all at once, or they can be used after each extraction, but after the individual extraction in order to effectively extract the active ingredient and to suppress the extraction of sub-components when mixing and extracting each material as much as possible. It is preferred to use mixed.

The cassia bark extract, phosphorus wormwood extract or a mixture thereof of the present invention has excellent antibacterial activity.

In one embodiment of the present invention, the detergent composition of the present invention may further include a yeast extract.

The yeast extract may be obtained by extraction and separation from commercial yeast using methods known in the art for extraction and separation, and the 'extract' defined in the present invention is extracted from yeast using an appropriate solvent, For example, it may be an extract extracted and purified by a conventional method by adding water or an organic solvent to the yeast.

The detergent composition of the present invention containing the yeast extract shows strong antibacterial / antifungal activity against fungi such as Candida albicans and Aspergillus brasiliensis, along with bacteria such as Staphylococcus aureus, E. coli and Pseudomonas aeruginosa.

The active ingredient extract of the present invention (one or more extracts selected from ginseng tree bark extract, sagebrush extract, and yeast extract) may be included in an amount of 0.01 to 20.0% by weight relative to the total weight of the detergent composition, and 1μg / ml to 10000μg / in the composition ml concentration.

In one embodiment of the present invention, the detergent composition may further include at least one selected from the group consisting of purified water, foaming agents, foam auxiliary agents, neutralizing agents, water softening agents, and antioxidants in addition to the active ingredients.

The detergent composition of the present invention can dissolve or disperse useful components including the remaining amount of water. At this time, it is preferable to use purified water as water, and other functions other than the function of the filler simply by being in a liquid phase or dissolved in a liquid solvent at room temperature. It may include a solvent having a. As such a solvent, one containing a solubilizing agent, an organic solvent and an electrolyte for phase stability, etc. may be used.

Examples of the solubilizing agent include polyethylene glycol, glycerin, propylene glycol, C1-C4 alkanol, alkylaryl sulfonate, carboxylic acid sulfate or sulfonate salt, urea, hydrocarboxylate, and carbohydrate having a weight average molecular weight of 100 to 2000. One or more selected from the group consisting of succinates can be used. As the alkanol having 1 to 4 carbon atoms, methanol, ethanol, propanol, isopropanol, and straight or branched butanol may be used.

Mono- or polyhydric alcohol may be used as the organic solvent. Specifically, carbon having 1 to 4 carbon atoms, methanol, ethanol, propanol, isopropanol, butanol, glycerin and mixtures thereof may be used.

As the electrolyte, salts such as sodium chloride, potassium chloride, sodium citrate, sodium acetate, and sodium sulfate may be used.

The 'foaming agent' referred to in this specification is a substance added to a solution or a raw material to form a stable foam, and a solution-based foaming agent is a type of surfactant. Examples include Sodium Laureth Sulfate (SLES), Sodium laurate, Sodium cocoyl isethionate, Sodium lauroyl sarcosinate, Sarcosia Anionic surfactants such as sarcosinates, potassium laurate, lauric acid and sulfosuccinates, glutamic acid; Lauryl Glucoside, Cocamido diethanolamide, alkyl polyglucoside, polysorbate20, PEG-100 Stearate and Polysorbate 60 60) nonionic surfactants such as; And amphoteric surfactants such as cocoamidopropyl betaine, and can be appropriately selected by a person skilled in the art.

In one embodiment of the present invention, as the foaming agent, sodium laureth sulfate (SLES), lauryl glucoside and cocoamidopropyl betaine may be mixed and used.

The 'bubble aid' referred to in this specification serves to enable the generation of minute and small bubbles in abundance when used with a foaming agent. In one embodiment of the present invention, as the foam auxiliary agent, a sunflower seed oil may be used, and when a sunflower seed oil is mixed with the foam agent, fine small bubbles may be abundantly generated.

The 'neutralizing agent' referred to herein is a pH adjusting agent for reducing irritation in the detergent composition, and may be one or more selected from sodium carbonate, sodium hydroxide, potassium hydroxide, triethanolamine, diethanolamine, and monoethanolamine.

The term 'hard water softening' referred to herein is a substance that acts to precipitate calcium or magnesium in hard water, remove it by ion exchange, or form a chelate to block the action of ions, such as ethylenediaminetetraacetic acid disodium Salt (C ₁₀ H ₁₄ O ₈ N ₂ Na _{2 ·} 2H ₂ O, CAS Number 6381-92-6) can be used.

The 'antioxidant' mentioned in this specification is a substance that acts as a natural preservative in addition to skin protection, and grapefruit extract may be used.

In addition, the detergent composition of the present invention may further include additives such as salts, preservatives, thickeners, enzymes, fragrances, dyes, emulsifiers, and metal encapsulants, the content of which is of ordinary skill in this field. It can be appropriately selected and adjusted by a ruler.

The salt or metal encapsulant is used to increase the formulation and phase stability of the detergent composition, and is generally a liquid alkali agent of monoethanolamine, diethanolamine, triethanolamine, or magnesium chloride, sodium sulfate, sodium carbonate, sodium silicate And one or more selected from the group consisting of magnesium sulfate.

The preservative may be phenoxy ethanol, formaldehyde solution, pentendiol, or the like.

The thickener is used to obtain a desired viscosity by blending an appropriate amount of an organic solvent and a thickener, which can satisfy the various needs of consumers by adjusting the viscosity according to the product when mixing and mixing each raw material into a product. As the thickener, a long chain fatty acid and polysaccharide containing sodium, potassium, aluminum, or magnesium as a salt may be used.

The perfume may be a commonly used aromatic compound, a substance that is chemically synthesized or extracted from natural materials, and is known in the art.

The detergent composition of the present invention is a kitchen detergent, floor detergent, toilet detergent, kitchen disinfectant, bathroom disinfectant, glass cleaner, fruit vegetable cleaner, oil remover, stain remover, bleach, furniture cleaner, laundry detergent for clothes, textile It can be used for various purposes such as softener, toilet cleaner, shampoo, conditioner, body cleanser, and toothpaste.

Hereinafter, the present invention will be described in more detail through examples. These examples are intended to illustrate the present invention more specifically, but the scope of the present invention is not limited to these examples.

<Example 1>

Preparation of plant extracts and yeast extracts

<1-1> Preparation of Cassia Leaf Extract

Fresh cassia ( Cercidiphyllum) from Hwacheon, Gangwon-do, August 2017 The leaves of japonicum were collected, dried in air for 2 weeks, and powdered to a fine powder for extraction. 2 kg of leaf powder was added to 4 L of alcohol (70% ethanol), reflux extraction was repeated 3 times at 70 ° C. for 3 hours, and foreign matter was removed by an ultrafilter (Nitto Denko, Japan). The filtered extract was concentrated under reduced pressure at 38 ° C as much as possible using a rotary evaporator, and then dried again using a freeze dryer. The cassia leaf extract obtained through this process was used as a sample in the following experiment while keeping refrigerated at 4 ° C.

<1-2> Preparation of Cassia Bark Extract

In May 2017, dried cassia bark was purchased from Donggwanghanjae, Sojeo-ro, Yeongju-si, Gyeongbuk. The purchased cassia bark was ground for extraction. After adding 2 kg of ground shell powder to 4 L of alcohol (70% ethanol), reflux cooling extraction was repeated 3 times at 70 ° C. for 3 hours, and foreign matter was removed by an ultrafilter (Nitto Denko, Japan). The filtered extract was concentrated under reduced pressure at 38 ° C as much as possible using a rotary evaporator, and then dried again using a freeze dryer. The cassia bark extract obtained through this process was used as a sample in the following experiment while refrigerated at 4 ° C.

<1-3> Preparation of phosphorus wormwood extract

Injin mugwort was purchased and used in May 2017 at Soje Herbal Street in Bongdam-eup, Hwaseong-si, Gyeonggi-do. After washing the ginseng mugwort 1 ~ 2 times with distilled water, dry it for 1 ~ 2 days at 20 ~ 60 ℃, put it in 4L of alcohol (70% ethanol) in 2Kg of dried ginseng mugwort and repeat reflux for 3 hours at 70 ℃ 3 times After extraction, foreign matter was removed with an ultrafilter (Nitto Denko, Japan). The filtered extract was concentrated under reduced pressure at 38 ° C as much as possible using a rotary evaporator, and then dried again using a freeze dryer. The extract of Injin mugwort obtained through this process was used as a sample of the following experiment while keeping refrigerated at 4 ℃.

<1-4> Preparation of yeast extract

5 kg of 80% aqueous ethanol solution was added to 1 kg of commercial yeast, extracted under reflux three times, and then deposited for 1 day at 15 ° C. Thereafter, the residue and the filtrate were separated by filtration and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 50 g of yeast extract.

<Example 2>

Detergent composition preparation

<2-1> Method for preparing detergent composition of the present invention

A detergent composition having the composition ratio of Table 1 below was prepared.

Composition, content and place of purchase of detergent composition Raw material name Content (% by weight) Where to buy Sunflower seed oil 1-10 Kalichem, taly Potassium hydroxide 0.1-5 Deoksan Chemical Foaming agent 10-50 Miwon Corporation, LG H & H Ethylenediaminetetraacetic acid, disodium salt 0.1-5 EDTA2Na, Deoksan Chemical Plant extracts 1-10 Direct production Yeast extract 1-10 Direct production Spices 0.1-5 HP AH22156, Hanbit fragrance Antioxidant (Grapefruit Extract) 0.1-5 Grapefruit seed extract,
Parchem, USA Purified water to 100 -

First, after heating the purified water to 60 ° C in the formulation batch, add sunflower seed oil, add potassium hydroxide, stir slowly, and stir sufficiently until the neutralization reaction is complete. Subsequently, sodium laureth sulfate (ES430 / Miwon Corporation), lauryl glucoside (Alkylpolyglucoside, APG / LG Living Health), and cocoamidopropyl betaine (Mitaine CA / Miwon Corporation) were sequentially added as foaming agents. Did. After confirming the complete dissolution, the temperature was lowered to 50 ° C, and the plant extract and / or yeast extract prepared in the above example were added to completely dissolve. Thereafter, the temperature was lowered to 40 ° C or lower, and then antioxidant and flavor were added to complete the kitchen detergent solution. Meanwhile, antioxidants used grapefruit extracts purchased from Parchem, USA, and HP AH22156, a product purchased from Hanbit spices, was used as a fragrance.

The prepared detergent composition may be filled in 500mL, 1000mL using an automatic filling machine, and then transported in a box packaging.

<2-2> Specific Preparation Examples of Detergent Composition of the Present Invention

The components and blending ratios of the specific preparation examples 1 to 7 of the detergent composition of the present invention are shown in detail in Table 2 below.

Specific Preparation Example of Detergent Composition Furtherance Manufacturing example (unit: weight%) T1 T2 T3 T4 T5 T6 T7 Sunflower seed oil 5 5 5 5 5 5 5 Potassium hydroxide 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Sodium laureth sulfate 20 20 20 20 20 20 20 Lauryl glucoside 10 10 10 10 10 10 10 Cockamidoflopilbetaine 10 10 10 10 10 10 10 Ethylenediaminetetraacetic acid, disodium salt 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Cassia leaf extract 5 - - 2.5 - 2.5 - Cassia bark extract - 5 - - 2.5 - 2.5 Phosphorus wormwood extract - - 5 2.5 2.5 2.5 2.5 Yeast extract - - - - - 3 3 Spices 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Antioxidant 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Purified water Balance Balance Balance Balance Balance Balance Balance Sum 100 100 100 100 100 100 100

<Example 3>

Antibacterial effect of the detergent composition of the present invention

The antimicrobial effect of the detergent compositions of Preparation Examples 1 to 7 prepared through Example <2-2> was tested.

The strains used for measuring the antibacterial activity of the composition of the present invention are Staphylococcus aureus , Escherichia coli , Pseudomonas aeruginosa ), Candida albicans ( Candida albicans ) and Aspergillus brasiliensis were used. On the other hand, for the culture of the strains, Staphylococcus aureus and Pseudomonas aeruginosa in TSB (trypticase soy broth) medium, E. coli in NA (Nutrient agar) medium, Candida albicans in YM agar medium, Aspergillus brasiliensis PDA ( potato dextrose agar) medium. Five strains to be tested were inoculated into a round bottom tube containing 5 mL of sterilized medium, and cultured at 37 ° C. for 18 to 22 hours, and absorbance was measured at 600 nm to confirm the degree of culture of the bacteria.

<3-1> Disc diffusion method

Dist diffusion method was used to measure the antibacterial activity.

After inoculating the 5 strains in a liquid medium, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were cultured at 37 ° C, and Candida albicans and Aspergillus brasiliensis were uniformly plated on a solid medium after incubation at 25 ° C. The sterilized paper disk (Advantec, 8 mm) was immersed in a 100-fold dilution of the detergent composition of the present invention through Example <2-2>, then saturated, dried, placed at regular intervals on a solid medium, incubated, and then around the disk. The diameter of the clear zone was measured.

The results are shown in Table 3 below.

Antibacterial effect of detergent composition according to the present invention sample Staphylococcus aureus Coliform Pseudomonas aeruginosa Candida Albicans Aspergillus brasiliensis Preparation Example 1 + + + + - Preparation Example 2 + +++ + + - Preparation Example 3 + ++ + + - Preparation Example 4 + + + + - Preparation Example 5 + +++ + + - Preparation Example 6 + + + +++ + Preparation Example 7 + +++ + +++ ++

<3-2> Minimum inhibitory concentration (MIC) measurement method

In this experiment, in order to confirm the antibacterial activity of the detergent composition of the present invention for the above 5 strains, the minimum inhibitory concentration (MIC) was measured. The measurement method was measured using a liquid medium dilution method. First, each detergent composition prepared in Preparation Examples 1 to 7 was diluted 10-fold in purified water, and then diluted in a 5 ml liquid medium by 28-fold steps. , Inoculated bacteria. The cells were cultured in an environment of 37 ° C., 5% CO ₂ for 3 hours, and the minimum concentration at which no strain growth was observed was determined as MIC. The results of MIC determination are shown in Table 4 below.

Antibacterial effect of the detergent composition according to the present invention (μg / ml) Strain Manufacturing example T1 T2 T3 T4 T5 T6 T7 Staphylococcus aureus 3.8 4.4 3.2 3.8 2.4 2.8 2.0 Coliform 2.8 1.2 2.8 3.0 1.2 3.0 0.8 Pseudomonas aeruginosa 3.8 3.4 3.4 3.8 4.0 3.8 3.4 Candida Albicans 3.2 3.8 3.4 3.4 3.2 1.2 1.0 Aspergillus brasiliensis - - - - - 4.8 2.0

As shown in Table 3-4, Preparation Examples 2, 5, and 7 of the present invention were found to have excellent antibacterial activity against gram-negative E. coli, and in the case of Preparation Examples 6 and 7, against the yeast Candida albicans The antibacterial activity was found to be excellent. Particularly, in Preparation Examples 1 to 5, the antibacterial activity against Aspergillus brasiliensis, which is a fungal bacterium, was not shown at all, but in Preparation Examples 6 and 7, antibacterial activity against the fungal bacteria was observed. It is thought that the yeast extract of the present invention showed an effect on controlling fungal bacteria.

Through these results, it is understood that the detergent compositions of the present invention have antimicrobial activity against various microorganisms, such as Gram-positive bacteria, Gram-negative bacteria, yeasts, and fungi, in case of Preparation Examples 6 and 7 Could. It was confirmed that the manufacturing example having the best antibacterial activity was 7.

<Example 4>

Determination of properties of detergent compositions of the present invention

Foam amount, cleaning power, rinsing property, skin irritation and safety of the detergent composition prepared according to the present invention were measured. On the other hand, as a control, a commercial product, Natural Pong (LG Health) was used.

<4-1> Foam amount evaluation

In order to measure the amount of foam, the stock solution of the detergent composition of the present invention prepared through Example <2-2> was diluted to a concentration of 0.1% by weight, put it in a cylinder of 100 ml, shaked 50 times, and the resulting foam Choose a method to measure the height of the quantity. The height of the foam amount was expressed by the numerical value of the volume displayed on the cylinder.

<4-2> Evaluation of cleaning power

In order to measure the amount of foam, a sample having 10 × 10 (cm ² ) sized rolling oil is deposited on the detergent composition of the present invention prepared through Example <2-2> for 10 seconds to degrease the rolling oil remaining on the surface of the sample. Did. After washing the degreased steel sheet with distilled water, the specimen was dried with a dryer, cut to a size of 10 × 2.5 (cm ² ), and the surface residual amount of the specimen was measured 5 times with an RC-212 carbon analyzer to obtain an average value.

<4-3> Skin irritation evaluation (lung wrap test)

The skin irritation test conducted in the present invention was applied to 5 healthy% of the detergent composition sample of the present invention diluted with distilled water inside the upper arm of each test group (T1-T5) for 5 healthy adults for 10 hours daily patch. After that, the skin irritation of the patch was evaluated. All skin irritation test values were compared with the skin irritation value by water, and the skin irritation by water was set as a criterion having a 'very good' value.

Decision code ● is no abnormality, ○ is slightly red than other skins, △ is a slight allergy symptom, × is a skin-specific symptom.

<4-4> Rinseability evaluation

The rinsing property is evaluated in three forms by measuring the amount of water used until the foam does not foam by washing a certain amount of tableware with a certain amount of the detergent composition of the present invention prepared through Example <2-2> on a sponge. A relative comparison was made.

The decision code × is not good, △ is normal, and ○ means very good.

<4-5> Stability evaluation

Stability evaluation was repeated for 6 cycles by repeatedly leaving the detergent composition of the present invention prepared through Example <2-2> for 12 hours at a low temperature of -10 ° C and 12 hours at a high temperature of 40 ° C. After the implementation, the apparent transparency of the detergent was evaluated as stability, and additionally, the foaming power and the cleaning power were evaluated.

Decision code ○ indicates no problem in appearance, foaming power and cleaning power, △ is cloudy in appearance, but there is no problem in foaming power and cleaning power, × means that there is a problem in appearance, foaming power and cleaning power.

Evaluation of the amount of foam, cleaning power, rinsability, skin irritation and stability of the detergent composition of the present invention sample Foam amount (ml) Cleaning power Skin irritation Rinsability Safety evaluation Preparation Example 1 94 90.9 ● ○ ○ Preparation Example 2 98 92.2 ● ○ ○ Preparation Example 3 92 92.7 ● ○ ○ Preparation Example 4 91 90.9 ● ○ ○ Preparation Example 5 94 91.5 ● ○ ○ Preparation Example 6 88 92.4 ● ○ ○ Preparation Example 7 89 92.8 ● ○ ○ Control 92 92.5 ● ○ ○

As shown in Table 5, the detergent composition of the present invention was able to obtain results for the amount of foam similar to conventional commercial products, cleaning power, skin irritation, rinsing properties, and stability of kitchen detergent.

<Example 4>

Evaluation of biodegradability of the detergent composition of the present invention

To determine the degree of biodegradability of the detergent composition prepared according to the present invention was performed as follows.

Using active sludge as a decomposition source, shake culture for 8 days at 23 ± 3 ℃ in a shake incubator containing a detergent composition to collect samples over a period of time, and then analyze the surfactant component of the sample to evaluate the biodegradability by analyzing the foam capacity. The activated sludge was an activated sludge collected from the Jungnang sewage treatment plant of the activated sludge method, and the concentration of the suspended material was adjusted to 10,000 to 20,000 mg / L. At this time, the composition of the biodegradability test incubator is as shown in Table 6 below, and the obtained results are shown in Table 7. At this time, the concentration of the detergent composition and the control of the present invention used was 30 mg / L.

Composition of incubator for biodegradability test Raw material Composition (g / L) Ammonium chloride 3.0 Dipotassium phosphate 1.0 Magnesium sulfate 0.25 Potassium chloride 0.25 Ferrous sulfate 0.002 Yeast extract 0.1 water 11

Biodegradability evaluation result sample Day 2 Day 4 Day 6 Preparation Example 1 82.2 96.5 99.9 Preparation Example 2 82.8 94.2 99.9 Preparation Example 3 83.5 96.6 99.9 Preparation Example 4 84.8 94.9 99.9 Preparation Example 5 85.3 94.3 99.9 Preparation Example 6 94.5 99.9 99.9 Preparation Example 7 94.8 99.9 99.9 Control 82.5 94.3 98.7

As can be seen from the results of Table 7, it was confirmed that the detergent composition of the present invention has a higher biodegradability compared to the conventional commercial products, and in particular, in the case of Preparation Examples 6 and 7, it was confirmed that the biodegradation activity is effectively made within a short period of time.

So far, the present invention has been focused on the preferred embodiments. Those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered in terms of explanation, not limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the equivalent range should be interpreted as being included in the present invention.

Claims (8)

As a detergent composition,
The composition includes a cassia bark extract, jinsug extract and yeast extract,
The composition has antibacterial activity against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa,
The composition is a detergent composition having antifungal activity against Candida albicans and Aspergillus brasiliensis. delete According to claim 1,
Detergent composition characterized in that the cassia bark extract, jinsukmu extract or yeast extract is contained in 0.1 to 10 parts by weight based on 100 parts by weight of the total composition. According to claim 1,
The composition is a detergent composition characterized in that it further comprises at least one selected from the group consisting of purified water, foaming agent, neutralizing agent, water softening agent, flavoring agent and antioxidant. According to claim 1,
The composition is a detergent composition characterized in that it has a liquid formulation. delete delete delete

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