CN109913324A - Utilize the functional detergent constituent of vine tea - Google Patents

Utilize the functional detergent constituent of vine tea Download PDF

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Publication number
CN109913324A
CN109913324A CN201811245711.XA CN201811245711A CN109913324A CN 109913324 A CN109913324 A CN 109913324A CN 201811245711 A CN201811245711 A CN 201811245711A CN 109913324 A CN109913324 A CN 109913324A
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CN
China
Prior art keywords
detergent
constituent
total weight
extract
vine tea
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Pending
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CN201811245711.XA
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Chinese (zh)
Inventor
王云新
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Che Ma Gun Investment (shanghai) Co Ltd
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Che Ma Gun Investment (shanghai) Co Ltd
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Publication of CN109913324A publication Critical patent/CN109913324A/en
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    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/382—Vegetable products, e.g. soya meal, wood flour, sawdust
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • C11D1/02—Anionic compounds
    • C11D1/12—Sulfonic acids or sulfuric acid esters; Salts thereof
    • C11D1/123—Sulfonic acids or sulfuric acid esters; Salts thereof derived from carboxylic acids, e.g. sulfosuccinates
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • C11D1/88—Ampholytes; Electroneutral compounds
    • C11D1/90—Betaines
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
    • C11D2111/10—

Abstract

The present invention relates to the functional detergent constituents using vine tea, then a little say in detail, to whole constituent total weight, wherein 0.1%~10% comprising Ampelopsis grossedentata extrat weight is characterized for functionality active component.According to the present invention, not only have cleaning effect similar with original synthetic detergent, while there are also Ampelopsis grossedentata extrat it is distinctive anti-oxidant and sterilization and antibacterial effect, can to avoid stimulation skin, it is free from environmental pollution the features such as.

Description

Utilize the functional detergent constituent of vine tea
[technical field]
The present invention is about vine tea functional detergent constituent, is to have superpower cleaning dynamics about utilization in more detail, And the vine tea functional detergent constituent with outstanding protection skin, sterilization, antibacterial and other effects.Belong to biological products technology Field.
[background technique]
Detergent constituent, which is applied, can be divided into domestic use and industrial use according to field difference, and especially domestic use can For kitchen use, laundry is subdivided into use, clean and use.In addition, synthetic detergent can be distinguished according to the type of feed of detergent and naturally washed Wash agent.
Especially recently, it as the consumer for pursuing health is continuously increased, is washed using the domestic bowl-washing of natural material The usage amount of agent is more and more.
In particular, the dosage of detergent also increases considerably recently as the increase of family kitchen or food and beverage enterprise's quantity. Moreover, also starting have higher requirement for dishwashing detergent, however, tradition washes the dishes with the enhancing to health understanding Machine detergent is being cleaned because not having the effect of the antibacterials such as safety and environmental protection, corrosion-free, intact skin or sterilization, this detergent The side effects such as harmful bacteria or virus or chemical residual on plate can be attached to when food.But vine tea functional detergent group At object tableware can be washed it is neat and tidy while also can corrosion-free human skin, moreover it is possible to play the role of healthy and safe.Therefore, The demand trend of natural detergent made by vine tea functional detergent constituent will become better and better.
Usual family kitchen detergent does not have the functional effects such as its antibacterial or sterilization other than cleaning effect.Make It is had been known or commonly to have been provided with the detergent for kitchen use of antibacterial effect, wherein including Nonoxynol-9 (Nonoxynol-9) although the detergent for kitchen use of antimicrobial component is manufactured and sold, stronger penetrating odor, by human body Absorption is extremely toxic, comes as kitchen detergent using unsuitable.Even if such cleaning agent is still attached to by food Harmful bacteria or virus on tableware cannot play effectively bactericidal effect.
On the one hand, usually to sterilize and clean bleaching water detergent used, although can kill about more than 50 it is thin Bacterium, but due to its stronger penetrating odor and when being absorbed by the body, generated substance is harmful to the human body and makes people unwilling Meaning uses.
On the other hand, clothes was contacted with human skin 24 hours one day, is especially pledged clothes and is still contained after washing Surfactant, fluorescent whitening agent, synthesis coloured material of detergent etc., will cause the chemical substance of adverse effect to human body, The symptom such as allergic dermatitis, eczema, dermatitis can be directly resulted in.Because the clothes washed using synthetic detergent, is equivalent to It is the same that chemical pollutant is smeared on skin, so selection detergent not only wants cleaning dynamics, it is often more important that select to have Detergent safe to the human body.Especially selection is for washing the detergent for contacting the clothes of delicate infant skin, safety Property cannot be ignored, should select non-stimulated to skin, plays bactericidal effect again.Therefore, consumer uses washing in selection Can more it pay attention to when the ingredient of agent to man body pollution or the smallest detergent of infection.
The problem of environmental pollution that chemical products detergent is influenced, human health safety problem are washed compared to chemical industry synthesis Agent, natural detergent articles are presently the most urgent development, nuisanceless high-performance made by natural environmental-protective plant material Cleaning detergent, not only there are antibacterial functions, while also will increase health, reduce chemical contamination body, play confrontation The effect of bacterium.
[advanced technical literature]
[patent document]
(patent document 0001) KR 10-1624951 B1
(patent document 0002) KR 10-1300632 B1
[summary of the invention]
The object of the present invention is to provide without using chemical combination substance but using natural materials cleaning force by force and have anti- The functional detergent constituent also low to skin irritatin of bacterium and bactericidal effect.
[project solution]
In order to achieve the above object, it is a feature of the present invention that account for entire detergent constituent using the extract of vine tea total The 0.1%~10% of weight is used as effective component.
Moreover, containing 0.1%~5% weight percent of fermenting ampelopsin grossedentata extract when surfactant as effective component 20%~30% weight percent, above-mentioned surfactant is from sodium sulfosuccinate labor human relations (Disodium Laureth sulfosuccinate；LES), olive surfactant, apple surface activating agent, cocoyl propylhomoserin potassium (Potassium Cocoyl Glycinate；PCG) and cocoyl-glycine betaine (Cocobetaine) in select a kind or more as feature.
Again include total weight 5%~15% organic acid, the essential oil of total weight 0.1%~0.5%, total weight 0.1%~ 5% water cress extract, the Distylium racemosum leaf extract and remaining sodium bicarbonate of total weight 0.1%~5% are as feature.
Above-mentioned constituent can be used as, and dishwashing detergent, toilet detergent, for kitchen use disappears at floor detergent Toxic agent, bathroom disinfectant, glass detergent, fruits and vegetables detergent, the detergent that deoils, stain scavenger, bleaching agent, furniture One or more of detergent, cloth-detergent, softening agent for fibres, toilet bowl cleaning agent are characterized.
[invention effect]
Detergent constituent of the invention is, while similar with previous synthetic detergent cleaning force, has vine tea The advantages that extract is distinctive anti-oxidant and bactericidal activity, and few to skin irritatin, free from environmental pollution.
[specific implementation summary of the invention]
The present invention is to provide for a kind of functional detergent constituent, assigns detergent using Ampelopsis grossedentata extrat and forms Object has functionality.The detergent constituent may include, and for kitchen use, laundry is used and all washings and detergent such as Clean- Type.Its type is not limited, and dosage form is it could also be possible that liquid or powder morphology.
Functional detergent constituent of the invention be with comprising Ampelopsis grossedentata extrat account for whole constituent total weight 0.1%~ 10% is characterized.
Herein, above-mentioned vine tea (Tengcha, scientific name: Ampelopsis grossedentata) is Vitaceae light leaf snake One kind of the wild stem post of grape, the referred to as kind of cogongrass cliff berry (moyeam).The major part of above-mentioned vine tea is grown in Zhangjiajie In the Yunwu Mountain Red Sandstone of 800~1500m high, germination autumn in spring fades, and does not have villus on stem and leaf, stalk diameter is about 0.2~0.4cm degree, peak annual output are early summer, followed by autumn.Such vine tea be missed on Compendium of Materia Medica it is dilute The treasure of some preciousnesses contains 14 kinds of 17 kinds of leucine, isoerine, methionine etc. of amino acid and potassium, calcium, zinc, selenium etc. Microelement, wherein flavones (Flavone), dihydromyricetin (DMY), anthocyanidin (OPC) equal size are very high.
More specifically, above-mentioned vine tea flavone content is 7.5~8.3% (minimum content >=6%), is the plant found so far Middle highest, thick protein contain 12.8%~13.8%, the content 25%~40% of dihydromyricetin.
The present invention is used after being extracted using the vine tea stem and leaf of picking, using the extract of vine tea, can be assigned Detergent constituent is anti-oxidant and antibacterial activity, and cleaning force and the sterilization, antibiotic property of detergent not only can be improved, also protection skin Skin mitigates stimulation of the detergent to skin.
The extraction of above-mentioned vine tea in the present invention, is extracted, cold soaking extraction using hot water, and heating hand-held is fetched stream cooling and mentioned It takes, ultrasonic wave extraction etc..In addition, said extracted can be carried out using solvent, solvent can be molten using water, ethyl alcohol or their mixing Matchmaker.
It further illustrates, said extracted can be taken in the dried powder of the stem in vine tea, leaf or their mixture and add 3~20 times of solvent of its weight, under the conditions of 20~100 DEG C, more appropriate is under the conditions of 40~90 DEG C by 30 points Clock~5 day are extracted, more appropriate to take 1~24 hour extracting method.Moreover, can be concentrated as needed, freeze to do Dry powdered.
Above-mentioned freeze-dried powdered is according to the known method in this technology fields, to this detailed description It can omit herein.
In addition, above-mentioned dried powder refers to the stem and leaf for picking above-mentioned vine tea, by drying, dry in the shade, heated-air drying etc. is square Method is dry, later in order to sufficiently extract the useful component of vine tea during the extraction process, carries out a degree of crushing, illustrates It says, is ground into 50~300mesh degree.Furthermore it is also possible to dry after crushing.
Above-mentioned Ampelopsis grossedentata extrat in the present invention is on the basis of the total weight of detergent constituent, which accounts for composition The 0.1%~10% of object total weight is best.Because if the very little antibacterial of its content, bactericidal properties effect are bad, if excessive bubble Foam effect etc. is just less good.
Moreover, detergent constituent of the invention can also include surfactant, above-mentioned surfactant is from sulfo group amber Meticortene Solu-Delta-cortef labor human relations (Disodium Laureth sulfosuccinate；LES), olive surfactant, apple surface activity Agent, cocoyl propylhomoserin potassium (Potassium Cocoyl Glycinate；) and cocoyl-glycine betaine (Cocobetaine) PCG The above are appropriate for middle a kind of selection.This is because, foam effect not only small to skin and eye irritation using natural surfactant Might as well, having faster biodegradability is environmental pollution in order to prevent.
In the present invention on the basis of detergent constituent total weight, the content of surfactant accounts for total weight 20%~30% To be moderate, because if the formation that its content will affect foam very little can be insufficient, not only economically without interests if excess, The decline of detergent overall quality can also be brought.
In addition, detergent constituent of the invention, accounts for the 0.1%~10% of total weight comprising Ampelopsis grossedentata extrat, surface is living Property accounts for the 20%~30% of total weight, while can further include fermenting ampelopsin grossedentata extract and account for total weight 0.1%~5%, because If having more excellent antibacterial, bactericidal effect the case where above-mentioned fermenting ampelopsin grossedentata extract to be further added by.
Here above-mentioned fermenting ampelopsin grossedentata extract refers to the weight in aforementioned Ampelopsis grossedentata extrat by 1: 1~10: 0.1~0.2 It gallisizes than adding, after fermentative microorganism is inoculated with 100,000~10,000,000cfu/L amount, in 20~40 DEG C of item It ferments 12~60 hours under part.
The above-mentioned fermentation liquid being fermented only uses the first-class liquid isolated by centrifuge separation.
Above-mentioned first-class liquid uses after can drying, if mentioned after dry using will add 1~20 times of dry amount Solvent is taken, in 4~30 DEG C of temperature conditions sinking 3~20 day time effective component extracting of product, is filtered, high pressure concentration and jelly Tie drying.
The extraction solvent that the solvent of said extracted can use industry conventionally used, but, at least to utilize more than one The solvent of water or ethyl alcohol composition extracts.
Above-mentioned fermentative microorganism using saccharomyces carlsbergensis (Saccharomyces) belong to it is more appropriate, more appropriate be using Saccharomyces cerevisiae (Saccharomycescerevisiae ATCC 18824), white sugar or oligosaccharide etc. can be used in above-mentioned sugar.
There are also detergent constituents of the invention to go back together with Ampelopsis grossedentata extrat, surfactant, fermenting ampelopsin grossedentata extract It can again include 5%~15% weight percent of organic acid, 0.1%~0.5% weight percent of essential oil, water cress extract 0.1%~5% weight percent, the sodium bicarbonate of 0.1%~5% weight percent of Distylium racemosum leaf extract when residual.
Above-mentioned organic acid is that also have bactericidal effect as the deodorization ingredient for eliminating stench, can be from malic acid, succinic acid 1 kind or more is selected to use in (succinic acid), tartaric acid, sorbic acid, ascorbic acid, lactic acid, acetic acid and gluconic acid.It is above-mentioned to have Machine acid content is in 5%~15% weight percentage ranges, because if the few then foam effect of its content is low, if it is excessive due to Its acidity will stimulate skin.
Above-mentioned essential oil plays the role of improving deodorizing effect as natural fragrance and antimicrobial component is provided.Such essence Oil can from lemon, lemon grass (Cymbopogon citratus) essential oil, green lemon essential oil, orange concentrated oil, sweet orange concentrated oil, grape concentrated oil, In Lavender, jasmine essential oil, Rosemary Oil, camomile tea essential oil, hamamelis concentrated oil and Yilan essential oil 1 kind of selection is used above.Above-mentioned essential oil content is in 0.1%~0.5% weight percentage ranges, if because its content is very little Effect can be very faint, less economical if excessive.
The extract for adding above-mentioned Chinese celery just has better sterilizing power and wash effect.Above-mentioned water cress extract is handle Extracting method of the dried powder of Chinese celery as Ampelopsis grossedentata extrat, is mentioned using water, ethyl alcohol or their mixed solvent, steaming liquid It takes, cold soaking extracts, heating is extracted, reflux cooling is extracted and ultrasonic wave extraction.At this moment, the extract of above-mentioned Chinese celery its effectively at Be dried in a known way in the range of point not being destroyed, for example, in the cool with the method for natural drying it is dry after, be The effective component crushing appropriate of Chinese celery is sufficiently extracted in later extraction process.Also, above-mentioned drying and crushing process foundation It needs to carry out or implement repeatedly with order change, naturally it is also possible to omit.
Above-mentioned water cress extract uses in the range of the 0.1%~5% of total weight, if its content is less than 0.1% effect Fruit is faint, as the increase for increasing its effect of content is unobvious if excess, and will appear and cannot ensure preparation stability Problem.
It will be more preferable into its sterilizing power of the extract of above-mentioned Distylium racemosum leaf and wash effect if added.Above-mentioned Distylium racemosum leaf Extract be the extracting method of the dried powder of Distylium racemosum leaf as Ampelopsis grossedentata extrat, using water, ethyl alcohol or they Mixed solvent, steaming liquid, which is extracted, cold soaking extracts, heating is extracted, reflux is cooling extracts, ultrasonic wave extraction.
At this moment, the extract of above-mentioned Distylium racemosum leaf is done in a known way in the range of its effective component is not destroyed It is dry, for example, in the cool with the method for natural drying it is dry after, sufficiently to extract having for Distylium racemosum leaf in later extraction process Imitate ingredient crushing appropriate.Also, above-mentioned drying and crushing process be according to needing to be carried out or implemented repeatedly with order change, It can certainly omit.
Above-mentioned Distylium racemosum leaf extract uses in 0.1%~5% weight percentage ranges, if content is less than total weight 0.1% its low effort, as the increase of the increase of content its effect is unobvious if excess, it may appear that cannot ensure preparation The problem of stability.
There are also sodium bicarbonates to use as neutralizer, if can generate precipitating using excessive, measures very few and has washing effect The bad worry of fruit.
In addition, detergent composition of the invention can also prepare any with dosage form of this field routine, neck is used Domain is unrestricted.Specifically, can be used for dishwashing detergent, floor detergent, toilet detergent, for kitchen use disappearing Toxic agent, bathroom disinfectant, glass detergent, fruits and vegetables detergent, the detergent that deoils, stain scavenger, bleaching agent, furniture Any one of detergent, cloth-detergent, softening agent for fibres, toilet bowl cleaning agent.
Hereinafter, the present invention is described in more detail by following experiment.But these examples are to illustrate this hair Bright, the scope of the present invention not only limits and this.
Case study on implementation 1: the preparation of Ampelopsis grossedentata extrat
The stem and leaf of vine tea are won by 1: 1 weight ratio, after cleaning, are dried in the shade to water content 15%, are ground into 100mesh degree.Next, adding the distilled water of 20 times of weight, extracted 24 hours under the conditions of 70~90 DEG C.Then it is used Filter paper filtering, it is freeze-dried after being concentrated under reduced pressure by rotary concentrator, produce powdered extract.
Case study on implementation 2: the preparation of Ampelopsis grossedentata extrat
The stem and leaf of vine tea are won by 1: 1 weight ratio, after cleaning, are dried in the shade to water content 15%, are ground into 100mesh degree.Along with the ethyl alcohol of 80% concentration of 10 times of weight, at 20 °C, stirring in 24 hours is extracted.Again by it It is filtered with filter paper, it is freeze-dried after being concentrated under reduced pressure by rotary concentrator, produce powdered extract.
Case study on implementation 3: the production of composite extract
The Ampelopsis grossedentata extrat and fermenting ampelopsin grossedentata extract of embodiment 1 are mixed by 1: 0.5% weight ratio.The extraction of embodiment 1 Water and white sugar are added on object by 1: 9: 0.1 weight ratio, by saccharomyces cerevisiae (Saccharomyces cerevisiae ATCC 18824) it by 100,000~10,000,000cfu/L inoculation, ferments 24 hours under conditions of 32 DEG C.After fermentation, by fermentation liquid It is centrifugated degerming, after ethyl alcohol is added until reaching 70% (V/V) concentration herein, every 5 hour refluxing extractions 3 times, after Filter.Then it is filtered with filter paper, it is freeze-dried after being concentrated under reduced pressure by rotary concentrator.
Embodiment 4: the preparation of composite extract
The Ampelopsis grossedentata extrat and fermenting ampelopsin grossedentata extract of embodiment 2 are mixed by 1: 0.5 weight ratio.At this moment, fermenting ampelopsin grossedentata Extract is to prepare in 3 method of embodiment, and Ampelopsis grossedentata extrat is prepared using 2 method of embodiment.
Test example 1: antioxidant effect measurement
Experiment and active oxygen are eliminated in order to confirm that the antioxidant effect of above-mentioned 1 to 4 extract of case study on implementation has done free radical Eliminate experiment.
Free radical eliminates experiment
Maximum absorbance is showed at 540 wavelength using stable DPPH (diphenyl hardship hydrazide group free radical), sample is wiped When except free radical DPPH and becoming Transparent color from purple, both with the increase of free radical elimination factor, absorptivity is at 540nm wavelength It reduces.And it is carried out by following tests method.
It is first that the 1Ml of 0.1mm DPPH (2,2-diphenyl-1-picryl-hydrazyl radical, Sigma) is molten Liquid is blended in the ethanol solution of 1Ml and dilutes, after being placed 15 minutes under conditions of 37 DEG C using microplate reader (UVT-06685, Thermo max, USA) absorbance is measured at 540nm wavelength.
It is that DPPH1Ml and methanol 1Ml is added that above-mentioned free radical, which eliminates the control group in test, is carried out with identical method Methanol 1Ml and sample 1Ml is added to obtain sample and the respective colour code corrected value of control group in measurement.
There are also free radical elimination factor is calculated using following mathematical formulae 1, its result is embodied in table 1.Following table 1 Middle SC50It is the concentration of sample required for eliminate free radical 50%, the smaller antioxidant activity of numerical value shows higher.
[mathematical expression 1]
Free radical elimination factor (%)=100- { (absorbance/control group absorbance of sample) × 100 }
Active oxygen eliminates experiment
It is reacted using xanthine/xanthine oxidation ferment (xanthine/xanthine oxidase, Sigma) ferment The active oxygen of generation utilizes the oxidimetry of the tetrazolium blue (nitroblue tetrazolium, NBT) occurred by active oxygen Absorbance, it will be appreciated that the eradicating efficacy of active oxygen.It is put into sodium carbonate (Na2CO3) 2.4Ml, xanthine (xanthine, Sigma) 0.1Ml, ethylenediamine tetra-acetic acid (EDTA, ethylenediamine tetraacetic acid) 0.1Ml, bovine serum albumin(BSA) (BSA, bovine serum albumin, Sigma) 0.1Ml, tetrazolium blue 0.1 and sample 0.1Ml, utilizes Scroll-tupe blender (Type 37600Mixer, Mini mix, USA) mixing, is placed 10 minutes under the conditions of 25 DEG C, is put into xanthine oxidation ferment (xanthine oxidase) 0.1Ml is simultaneously reacted after twenty minutes under the conditions of 25 DEG C, is put into 6Ml copper (CuCl2) and is terminated instead It answers, measures absorbance at 540nm wavelength using microplate reader (UVT-06685, Thermo max, USA).
Control group in above-mentioned active oxygen elimination activity test is to replace sample to survey using identical method with 3 distilled water It is fixed, 3 distilled water are put into instead of xanthine oxidation ferment (xanthine oxidase) solution and are extracted, and are set as obtaining to sample With the respective colour code corrected value of control group.
Active oxygen is calculated followed by following mathematical expression 2 and eliminates rate score, its result is embodied in table 1.In table 1 IC50It is for sample concentration required for Scavenging active oxygen 50%, numerical value is smaller to mean that antioxidant activity is stronger.
[mathematical expression 2]
Active oxygen elimination factor (%)=100- { (absorbance/control group absorbance of sample) × 100 }
[table 1]
The result of test example 1
It distinguishes Free radical elimination factor SC50 (mg/ml) Active oxygen elimination factor IC50 (mg/ml)
Experimental example 1 0.142 0.081
Experimental example 2 0.131 0.072
Experimental example 3 0.113 0.057
Experimental example 4 0.101 0.053
It has been confirmed that extract according to the present invention shows very outstanding antioxidant effect in such as above-mentioned table 1.
Test example 2: antimicrbial power measurement
In order to verify the antibacterial effect of above-mentioned 1 to 4 extract of implementation, antibacterial tests are carried out using paper disc method.
As experimental strain, using gram-positive bacteria with staphylococcus aureus (Staphylococcus aureus KCTC 6910), Gram-negative bacteria is with pseudomonas aeruginosa (Pseudomonas aeruginosa KCTC 1637), large intestine Bacterium (E.Coli KCTC1039), yeast is with candida albicans yeast (Candida albicans KCTC 7965), and der Pilz is with aspergillus More than bacterium (Aspergillus niger KCTC 6910) 5 kinds of bacterial strains.
Each bacterial strain cultivated on plating medium takes 1 platinum loop to be put into culture solution 10Ml and cultivates 24 hours, makes its work Jump, then 0.1Ml bacterium solution is inoculated into culture solution 10Ml again and is cultivated 6 hours, it is equably smeared with sterile paint daubs, makes to be inoculated with Reach each plating medium bacterium solution about 10CFU/Ml.
Later, the paper disc (6mm, Satorius, Germany) for having killed bacterium is placed on solid plate culture medium, then The sample for being dissolved in solvent is absorbed into 0.05Ml/ disk and cultivates.Staphylococcus aureus (Staphylococcus Aureus), green pus bacterium (Phseudomonas aeruginosa), coliform (E.Coli) under the conditions of 37 DEG C, fungal species Candida albicans yeast (Chandida albicans), Aspergillus (Aspergillus niger) is under the conditions of 27 DEG C of temperature, respectively From culture 24 hours, 120 hours, and around Measuring plate transparent region diameter.As positive control, uses and be known as antibacterial The outstanding methanol bactericidal agent of activity, is as a result embodied in table 2.At this moment, the transparent region around disk is that opposite strain growth inhibits The index of degree, diameter mean that more greatly antibacterial effect is higher.
2 result of [table 2] experimental example
It has been confirmed that the methanol of fungicide of the extract according to the present invention than being known as strength kills in such as above-mentioned table 2 Microbial inoculum has more bactericidal activity.
Prepare embodiment 1 to 4: the preparation of detergent constituent
Of the invention prepares the detergent constituent containing embodiment 1 to 4 with usual way by following component ratio. Production example 1 is that extract 20g, the LES 50g, citric acid 20g and lemon of embodiment 1 are added into 109g sodium bicarbonate 1g is prepared after stirring.
It prepares the method identical with production embodiment 1 of embodiment 2 to implement, but 20g sodium bicarbonate is changed into 10g Chinese celery and is mentioned Object is taken to add 10g Distylium racemosum leaf extract.
Embodiment 3,4 and production embodiment 1,2 same methods are prepared, but the extract of embodiment 1 is changed into embodiment 3 Extract come using.
Above-mentioned water cress extract is the water that 15 times of weight is added into Chinese celery, is heated under the conditions of 80~100 DEG C of temperature It after 20 hours, is concentrated under reduced pressure under the conditions of 50 DEG C after filtering using reduced pressure machine, freeze-dried rear the powdered of acquisition mentions Take object.
Above-mentioned Distylium racemosum leaf extract is that Distylium racemosum leaf is dried in the shade to make to reach moisture content lower than 10%, and 15 are added wherein The water of times weight carries out 15 hours hot water under the conditions of 80~90 DEG C of temperature and extracts.Then filtering recycles decompression compressor It is concentrated under reduced pressure under the conditions of 50 DEG C of temperature, the powdered extract obtained after freeze-dried.
Compare preparation embodiment 1
Method preparation identical with above-mentioned preparation embodiment 1, only replaces the extract of embodiment 1 to mix with sodium bicarbonate It closes.
Test case 3: foam volume, cleaning force, skin irritation, rinsability evaluation
The synthesis kitchen detergent of commercial type has been used in test case 3 as a control group.
Foam volume
Above-mentioned preparation embodiment 1 to 4 is utilized in order to measure foam volume and compares the detergent group of the preparation preparation of embodiment 1 At object, being diluted with water to concentration is 0.1% (weight ratio), and it is 100ml graduated cylinder that 10ml detergent composition, which is then put into capacity, In.And shake 50 times, measure the height of the foam of generation.The amount of foam is embodied with the volume that shows in graduated cylinder.
Cleaning force evaluation
In order to which the evaluation of cleaning force by above-mentioned preparation embodiment 1 to 4 and compares the detergent composition for preparing embodiment 1 and preparing Object is put into 10 × 10cm2The test piece of the contamination steel rolling oil of size deposits 10 seconds, carries out degreasing to steel rolling oil remained on surface.? The steel plate of degreasing afterwards with the dry test piece of drying machine, is cut into 10 × 2.5cm wash with distilled water2Size, with RC-212 carbon analysis machine Measurement strip residual quantity 5 times, then obtains its average value.
Skin irritation evaluation
With above-mentioned preparation embodiment 1 to 4 and to compare preparation real on healthy men and women each 10 upper arm upper covers bubbles (patch) Behind detergent constituent 5 hours for applying the preparation of example 1, them is allowed to evaluate the skin irritation at cover bubble position.All skins Skin stimulation test value and water are compared the stimulation of skin, and water is set as the value of the stimulation of skin ' very good '.
Decision symbol are as follows: ● it is without any exception, zero is to have slightly red phenomenon than other skins, and △ is that slight mistake occurs Quick property reaction, × it is to have special phenomenon on skin.
Rinsability evaluation
Rinsability evaluate is above-mentioned preparation embodiment 1 to 4 and to compare the detergent constituent for preparing embodiment 1 and preparing A certain amount of be applied on sponge cleans a certain amount of tableware, the water used until foam until it is invisible is measured, in the form of 3 kinds Corresponding comparison is done.
Decision symbol: × be it is bad, △ be it is common, zero is very good.
Then result is embodied in table 3.
3 result of [table 3] experimental example.
It distinguishes Foam volume (ml) Cleaning force Skin irritation It is rinsability
Prepare embodiment 1 90 90.1 ● ○
Prepare embodiment 2 91 91.2 ● ○
Prepare embodiment 3 90 93.3 ● ○
Prepare embodiment 4 92 94.5 ● ○
Compare preparation example 1 82 56.1 ○ ○
Control group 86 90.5 △ △
As, it has been confirmed that preparation embodiment 1,2 according to the present invention is compared with synthetic detergent, having good in table 3 Foaming characteristic and good rinsability and detergency, while there is less skin irritation.
More than, be described in detail by the present invention using appropriate case study on implementation, but the scope of the present invention be not limited solely to it is specific Within case study on implementation, it should be explained further according to additional patent claim.Moreover, having if it is in this technical field The people of common knowledge, it should be appreciated that can also have a possibility that many modifications and variations under the premise of not departing from the present invention.

Claims (2)

1. using the functional detergent constituent of vine tea, it is characterized in that using the total weight of functional detergent constituent as base Standard, using 0.1-10% effective component of the Ampelopsis grossedentata extrat in constituent total weight, fermenting ampelopsin grossedentata object is in constituent total weight In 0.1-5%, surfactant 20%~30% weight in constituent total weight, organic acid is 5% in constituent total weight ~15%, essential oil 0.1%~0.5% in constituent total weight, water cress extract 0.1%~5% in constituent total weight, Distylium racemosum leaf in constituent total weight 0.1%~5% and residual sodium bicarbonate be effective component.
The Ampelopsis grossedentata extrat of 1: 1~10: 0.1~0.2 weight ratio, water and sugar are added in the above fermenting ampelopsin grossedentata extract, herein The fermentative microorganism of inoculation 100,000~10,000,000cfu/L, ferments 12~60 hours under conditions of 20~40 DEG C, sends out Zymotic fluid, using its separated first-class fermentation liquid out, makes the functional detergent constituent of vine tea after centrifuge separation.
2. constituent according to claim 1, it is characterised in that composition described above object is with dishwashing detergent, floor with washing It washs agent, toilet detergent, disinfectant for kitchen use, bathroom disinfectant, glass detergent, fruits and vegetables detergent, go oil wash Agent, stain scavenger, bleaching agent, Furniture detergent, cloth-detergent, softening agent for fibres and toilet bowl cleaning agent etc. are therein 1 kind of functional detergent constituent using vine tea being characterized.
CN201811245711.XA 2017-10-23 2018-10-23 Utilize the functional detergent constituent of vine tea Pending CN109913324A (en)

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