KR101577118B1 - Cosmetic Composition for Moisturizing Containing Red Ginseng Butter as an Active Ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising red ginseng butter, which comprises extracting red ginseng oil, purifying the red ginseng oil, and curing the extracted red ginseng oil by hydrogenation reaction, Red ginseng butter does not contain heavy metals and harmful components, exhibits a moisturizing effect and antioxidant effect, and has a melting point of about 40 degrees, which is suitable for use in cosmetic emulsion formulations. Accordingly, the red ginseng butter according to the present invention can be used as an active ingredient of a cosmetic composition for moisturizing or antioxidizing.

Description

[0001] The present invention relates to a cosmetic composition for moisturizing cosmetic composition containing red ginseng butter as an active ingredient,

The present invention relates to a cosmetic composition for moisturizing or antioxidation containing red ginseng butter as an active ingredient.

The skin is divided into three layers of epidermis, dermis and subcutaneous fat tissue in order from the outside, and functions to protect the human body from physical and chemical stimulation from external environment. The stratum corneum of the epidermis contains about 10 to 20% of water, and is present at the outermost part of the human body, thereby suppressing evaporation of water to the outside of the body, while blocking excessive material penetration from the outside. These stratum corneum surfaces are wrapped with a thin natural protective film made of sebum from the sebaceous glands and perspiration from the sweat glands, which can prevent water evaporation.

The cells that make up this stratum corneum have a high concentration of Natural Moisturizing Factor (NMF), which is a water-soluble component, which helps the skin to maintain flexibility and maintain proper moisture. For example, The material is not only water-soluble, but also effectively combines with moisture to inhibit moisture from drying out of the skin. However, the water content of the stratum corneum is decreased due to various factors such as an artificial temperature control of the cooling / heating according to the change of the environment or the life pattern, various stresses caused by the social life, skin stress due to environmental pollution, The need for a skin moisturizing agent is increasing because the skin becomes dry, the surface becomes rough, and the skin becomes lustrous and appears dull.

Conventionally, a method of increasing the moisture retention in the stratum corneum by using a humectant having a property of absorbing moisture as a humectant and an occlusive moisturizer preventing evaporation of water has been most widely used. Most of the hu martens are polyols such as glycerin, propylene glycol, 1,3-butylene glycol, and polyethylene glycol. However, they are disadvantageous in that they have a high stickiness due to the inherent viscosity and physical properties of the polyol when applied to the skin. In addition, the formulations containing the lamellar liquid crystals, which have been undergoing much research until recently, are known to have excellent moisture retention due to their similar merits to the keratinous structures of the skin, but they can be manufactured only in a specific composition of the raw materials and thus have problems in universal use.

On the other hand, free radicals are very chemically reactive chemicals. They are caused by the external factors such as ultraviolet rays, radiation, exhaust gas, tobacco smoke and stress in the human body and the metabolic processes of mitochondria and leukocytes in the body And the lipid peroxidation, which is a component of the cell membrane, is peroxidized through a multistage chain reaction (Patent Document 1). If free radicals accumulate in the body excessively, it will cause various human diseases such as aging of cells as well as inflammation, stroke, myocardial infarction, diabetic vascular disorder, hyperlipidemia, diabetes and neurodegenerative diseases. In addition, free radicals are known to be associated with skin aging.

The eradicants capable of removing the free radicals have been reported to have the effect of preventing or treating related diseases, and studies for developing various active oxygen (or free radical) eradication materials from microorganisms and plants have been continued.

Panax Ginseng is a herbal medicine used in Korean medicine and ginseng, which has been widely used in traditional medicine as well as folk remedies. It has been used for a long time as a medicinal material and proved its safety and efficacy. Red ginseng is manufactured by steam - drying the roots of ginseng. It has been reported that the ginseng has a protective effect against radiation, a chromosome abnormality and micro-nuclear defense effect by an alkylating agent, mutation reduction in cultured cells, and reduction of cell deformation. In addition, red ginseng acidic polysaccharide (RGAP) isolated from red ginseng has been reported to have anti-cancer immunomodulating effect (Patent Document 2).

As described above, although various physiological effects of ginseng or red ginseng have been confirmed, the moisturizing effect and antioxidative effect of red ginseng butter produced by curing the oil derived from red ginseng have not been disclosed.

Korean Patent No. 10-1117563 Korean Patent Application No. 2001-33115

An object of the present invention is to provide a moisturizing cosmetic composition comprising red ginseng butter as an active ingredient.

Another object of the present invention is to provide an antioxidant cosmetic composition containing red ginseng butter as an active ingredient.

In order to solve the above problems, the present invention provides a cosmetic composition for moisturizing comprising red ginseng butter as an active ingredient.

In addition, the present invention provides an antioxidant cosmetic composition comprising red ginseng butter as an active ingredient.

Hereinafter, the present invention will be described in detail.

The red ginseng butter may be manufactured in the following steps, but is not limited thereto:

1) extracting red ginseng oil;

2) purifying the extracted red ginseng oil; And

3) Curing the purified red ginseng oil.

The red ginseng oil according to the present invention can be obtained from red ginseng powder or red ginseng powder.

The red ginseng is prepared by a conventional process, and the red ginseng powder is obtained by extracting the extract from red ginseng, washing the obtained red ginseng with water, and then drying it by hot air drying.

The red ginseng oil of the present invention can be prepared by conventional methods known in the art such as room temperature extraction, warming extraction, ultrasonic extraction, supercritical extraction, and the like using red ginseng or red ginseng powder.

Preferably, the red ginseng oil refers to an extract obtained through supercritical extraction without the non-polar solvent extraction fraction or the auxiliary solvent of red ginseng.

Specifically, a non-polar solvent is used to effectively extract the lipid-soluble plant sterol from red ginseng.

The nonpolar solvent used in the present invention is hexane, chloroform, dichloromethane, ethyl acetate and mixed solvents thereof. Preferably, hexane is used.

This extraction is carried out by adding 50 ml to 1 L of a non-polar solvent to 1 g of red ginseng or red ginseng powder and performing the reaction at 4 to 70 캜 for 30 minutes to 24 hours, preferably at 25 to 65 캜 for 30 minutes to 2 hours.

Further, in order to obtain a high extraction yield, it is possible to extract in a supercritical condition without an auxiliary solvent. Supercritical extraction is performed by putting red ginseng powder or red ginseng powder into a supercritical extraction tank, supplying supercritical fluid (for example, carbon dioxide), maintaining the pressure of the extraction tank at 150 to 500 bar, and performing the autoclave at 30 to 90 ° C for 0.5 to 10 hours do. The supercritical extraction is preferably carried out at a pressure of 175 to 450 bar at 55 to 85 ° C for 1 to 8 hours, more preferably at a pressure of 200 to 400 bar at 60 to 80 ° C For 1 to 6 hours, but is not limited thereto. The red ginseng oil is extracted using supercritical extraction conditions except that the pressure of the extraction tank is maintained at 150 to 500 bar and the filtration is carried out at 30 to 90 ° C for 0.5 to 10 hours, , There is a problem that the obtained red ginseng oil does not exhibit the antioxidative effect as desired.

At this time, the supercritical fluid may be one selected from the group consisting of carbon dioxide, nitrogen, nitrous oxide, methane, ethylene, propane and propylene. Preferably, carbon dioxide is used.

The red ginseng oil thus obtained is purified if necessary. Such tablets are not particularly limited in the present invention, and known methods are possible. For example, ion exchange resin or silica gel column chromatography can be carried out after filtration under reduced pressure, and purification can be carried out by fractional distillation, more preferably fractional distillation can be carried out.

The fractional distillation may be performed under the conditions of a degree of vacuum of 0.1 mbar to 2 mbar and a temperature of 180 ° C to 280 ° C and preferably a vacuum degree of 0.1 mbar to 1 mbar and a temperature of 200 ° C to 260 ° C, 0.1 mbr to 0.6 mbar, and a temperature of 220 ° C to 240 ° C. However, the present invention is not limited thereto. If the degree of vacuum is less than 0.1 mbar or the temperature is less than 180 캜, a desired acid value can not be obtained. If the degree of vacuum is 2 mbar or more and the temperature is 240 캜 or more, the antioxidative effect of red ginseng oil is reduced.

Red ginseng oil extracted and purified under the above conditions has a dark brown specific odor and contains 45 to 55% of linoleic acid, an acid value of 5.0 or less, an iodine value of 8 to 15, and a heavy metal such as mercury, lead, cadmium, and antimony at 20 ppm Or less, and arsenic is detected as 2 ppm or less. When the red ginseng oil is produced within the range outside the extraction and purification conditions described above, the above-mentioned overall characteristics may not be satisfied, for example, the acid value may be high, the content of linolenic acid may be low, the iodine value may be low, Heavy metal levels can be high.

In this regard, higher acid values can lead to acidosis, lower linolenic acid content, and lower iodine content, which may reduce the usefulness of the skin to the skin due to the reduced content of unsaturated fatty acids useful in the skin. In addition, when the amount of heavy metals is high, it can not be used as a cosmetic or pharmaceutical raw material.

Therefore, when the red ginseng oil is prepared outside the range of the extraction and purification conditions described above, the red ginseng oil is not satisfactory in its overall properties, and the desired moisturizing effect and antioxidant effect can not be obtained. Therefore, There is a problem unsuitable for the following.

In an embodiment of the present invention, a pressure of 250 to 300 bar is applied, extraction is carried out at a temperature of 65 to 75 ° C for 3 hours and then distillation is performed at a vacuum degree of 0.1 to 0.6 mbar and a temperature of 220 to 240 ° C. The obtained red ginseng oil has a lower acid value than the red ginseng oil extracted only and has a remarkably superior antioxidative effect as compared with other plant-derived oils.

The red ginseng oil thus prepared contains various components, preferably 80% or more by weight of the main ingredient, 0.1 to 10% by weight of the phytosterol, 0.1 to 10% by weight of the phospholipid and 0.1 to 5% And polyacetylene.

Wherein the fat comprises a C 6 to C 24 saturated or unsaturated fatty acid as a main component and the ratio of each fatty acid to 100 parts by weight of the fat is 0.05 to 5 parts by weight of the C 14: 0.05 to 5 parts by weight of C16: 1 palmitoleic acid, 0.01 to 5 parts by weight of C18: 0 stearic acid, 1 to 10 parts by weight of C18: 1 oleic acid, 30 to 30 parts by weight of C18: 2 linoleic acid, But is not limited to, a fat consisting of 80 parts by weight of the C18: 3 linolenic acid, 0.1 to 5 parts by weight of the C18: 3 linolenic acid, and 0.05 to 5 parts by weight of the C20: 0 arachidic acid.

In one embodiment of the present invention, for each 100 parts by weight of the fat, each fatty acid is included in the following proportions: C14: 0 Misteroolic acid (0.2 parts), C16: 0 palmitic acid (19.7 parts) (1.1 parts by weight), C18: 1 oleic acid (4.1 parts by weight), C18: 2 linoleic acid (61.7 parts by weight), C18: 3 linolenic acid (2.8 parts by weight) , C20: 0 arachidic acid (1.1 parts by weight), and at least 50 parts by weight thereof is composed of linoleic acid (see Table 2).

In step 3), the red ginseng oil may be cured by hydrogenation reaction. As the catalyst, nickel, platinum, palladium, rhodium, ruthenium and the like may be used. Nickel may be preferably used.

The hydrogenation reaction may be carried out at a wide temperature range from room temperature to 500 ° C, preferably from 100 ° C to 400 ° C, more preferably from 180 ° C to 300 ° C, It does not.

The hydrogenation reaction may be carried out under a pressure of 1 atm to 4 atm, preferably at a pressure of 2 atm to 3.5 atm, more preferably at a pressure of 2 atm to 3 atm, but is not limited thereto.

The hydrogenation reaction may be carried out for 30 minutes to 200 minutes, preferably 50 minutes to 150 minutes, more preferably 80 minutes to 120 minutes, but is not limited thereto .

In one embodiment of the present invention, when the purified oil was subjected to hydrogenation reaction using nickel as a catalyst for 100 minutes under a pressure of 220 캜 and 2.5 atm, it was confirmed that the red ginseng oil was cured and red ginseng butter having a melting point of 38 캜 was obtained (See Example 2)

The red ginseng butter may be contained in an amount of 0.1 to 20 parts by weight, preferably 1 to 15 parts by weight, more preferably 1 to 10 parts by weight, based on 100 parts by weight of the moisturizing cosmetic composition. But is not limited thereto.

In addition, the present invention provides an antioxidative cosmetic composition comprising the red ginseng butter as an active ingredient.

The red ginseng butter may be contained in an amount of 0.01 to 20 parts by weight, preferably 0.05 to 15 parts by weight, more preferably 0.07 to 10 parts by weight, based on 100 parts by weight of the antioxidative cosmetic composition. But is not limited thereto.

The cosmetic composition may include, but is not limited to, a formulation of a softening agent, a nutritional lotion, a nutritional cream, a massage cream, an essence, a cleansing cream, a cleansing foam, a pack, a body oil or a powder.

The type of cosmetics to which the red ginseng butter of the present invention can be applied is not particularly limited and can be applied to all conventional formulations. For example, they may be formulated as solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations and sprays, But is not limited thereto. More specifically, it may be formulated into a formulation of softening agent, nutritional lotion, nutritional cream, massage cream, essence, cleansing cream, cleansing foam, pack, body oil or powder.

The cosmetic composition of the present invention may further contain other components which can give a synergistic effect to the effect of the red ginseng oil within the range not impairing the moisturizing effect in addition to the red ginseng butter. For example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, or carriers.

When the formulation of the present invention is an emulsion such as an essence, lotion or cream, besides red ginseng butter, oil such as squalene, mineral oil, caprylic / capryl triglyceride, dimethicone, and wax components such as stearyl alcohol and beeswax , Polyhydric alcohols such as glycerin and propylene glycol, thickeners, moisturizers, perfumes and the like. In the case of solubilizing formulations such as essence, skin, mist, etc., it may contain solubilizing components such as PEG-40 hydrogenated castor oil and thickening agent, moisturizing agent, perfume etc. in addition to red ginseng butter.

When the formulation of the present invention is a massaging cream, it may further contain an oil such as liquid paraffin, petrolatum, isononyl, sononanoate, etc., and the pack may be a peel off pack containing polyvinyl alcohol, Type cosmetics can be prepared with wash off packs containing pigments such as kaolin, talc, zinc oxide, titanium dioxide and the like.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amides Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

The moisturizing cosmetic composition containing red ginseng butter according to the present invention can be prepared by adding a common ingredient to ordinary skin formulations such as oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a pigment, a preservative, It is possible to mix and apply as necessary.

The present invention relates to a cosmetic composition containing red ginseng butter, which comprises extracting red ginseng oil, purifying the red ginseng oil, and curing the extracted red ginseng oil by hydrogenation reaction, Red ginseng butter does not contain heavy metals and harmful components, exhibits a moisturizing effect and antioxidant effect, and has a melting point of about 40 degrees, which is suitable for use in cosmetic emulsion formulations.

Hereinafter, the present invention will be described in detail with reference to Examples, Experimental Examples and Preparation Examples.

However, the following examples, experimental examples and preparation examples are for illustrating the present invention, and the content of the present invention is not limited by the following examples, experimental examples and production examples.

< Manufacturing example  1> Supercritical  Preparation of red ginseng oil using extraction

One kilogram of red ginseng was put into a supercritical extraction tank, and the reactor was sealed. Liquid carbon dioxide, which had passed through a condenser, was introduced into the extraction tank through a conduit at 30 SFC / min. Subsequently, a pressure of 250 to 300 bar was applied, and the temperature of the extraction tank was maintained at 65 to 75 ° C., followed by extraction for 3 hours to obtain red ginseng oil. The extraction yield was 0.8 to 0.9%.

The extracted oil was firstly heated at 120 ° C to 150 ° C before distillation. The mixture was degassed after the first heating and distilled by heating at a vacuum degree of 0.1 to 0.6 mbar and a temperature of 220 to 240 ° C using a thin film molecular still (VTA, Model VK83-6, Germany). The yield was 62%.

< Comparative Example  1> Hexane  Or oil-soluble oil

<1-1> Hexane  Manufacture of red ginseng oil

600 mg of hexane was added to 10 g of red ginseng powder and refluxed at 65 캜 for 60 minutes to extract a fat soluble component. The obtained filtrate was subjected to removal of hexane under reduced pressure to obtain a dark red ginseng oil.

<1-2> Preparation of red ginseng oil using oil-soluble oil (1)

After the red ginseng was immersed in 5 g of the cottonseed oil developed for cosmetics, red ginseng was extracted for 8 hours by using an ultrasonic extractor to obtain fused oil of red ginseng.

<1-3> Preparation of red ginseng oil using oil-soluble oil (2)

Red ginseng was obtained by immersing red ginseng in 5 g of baba coconut oil developed for cosmetics and then extracting red ginseng for 8 hours by using an ultrasonic extractor.

< Example  1> Manufacturing example  Check the acidity of red ginseng oil of 1

The acid value of red ginseng oil before and after fractionation was measured five times and it was confirmed that the acid value of red ginseng oil was improved (Table 1).

1 time Episode 2 3rd time 4 times 5 times Average Acid value before distillation 25.21 24.89 25.17 25.45 24.85 25.11 Acid value after distillation 0.99 1.09 1.09 0.89 0.89 0.99

As shown in Table 1, the acid value before distillation was about 25.11, while the acid value of distilled red ginseng oil was reduced to about 0.99. By lowering the acid value through fractional distillation, it is possible to improve the discoloration / de-oiling due to the acid clogging of the oil occurring in the higher acid.

< Example  2> Curing of red ginseng oil through hydrogenation reaction

0.18% of nickel was added as a catalyst in order to conduct hydrogenation reaction to red ginseng oil of Production Example 1 and Comparative Examples 1-1 to 1-3. Oil was added to a batch reactor, Hydrogenation reaction was carried out for 100 minutes. After completion of the reaction, the reaction product was purified using a filter paper, and solidified at room temperature to prepare butter. The contents of fatty acids and degree of curing of each oil are shown in Table 2 below.

　
Type and content of fatty acids Buttering Melting point
(° C) C8: 0 C10: 0 C12: 0 C14: 0 C16: 0 C16: 1 C18: 0 C18: 1 C18: 2 C18: 3 C20: 0 Red ginseng
oil 0 0 0 0.2 19.7 0.2 1.1 4.1 61.7 2.8 1.1 possible 38 Comparative Example 1-1 0 0 0.46 0.82 38.2 0 5.32 42.5 12.2 0.1 0.39 possible 40 Comparative Example 1-2 5.34 4.25 35.9 13.3 11.1 0 23.7 3.77 2.71 0 0 impossible 25 Comparative Example 1-3 6.01 5.11 43.9 16.3 9.04 0 3.51 13.7 2.43 0 0 impossible 20

As a result, the supercritical extraction method and the solvent extraction method had a higher melting point than the room temperature after the hydrogenation reaction, so that buttering was possible. However, in the case of other oil-soluble oils, the lipid component ratio varies depending on the base oil. . And that even when hydrogen is added, the type and content of the fatty acid contained in each oil differ depending on the method of extracting the oil, and it has been found that the oil may not be buttered.

In the case of Comparative Example 1-1, buttering is possible, but hexane and the like are used to extract the oil, and in the case of a solvent having a low polarity such as hexane, a portion that is difficult to be commercialized due to skin side effects due to residual solvents have. On the other hand, the red ginseng oil prepared in Preparation Example 1 is more suitable for use as an active ingredient of a cosmetic composition for moisturizing or antioxidation because it can be buttered while using supercritical extraction and the risk of residual solvent is reduced Do.

< Manufacturing example  3> Preparation of emulsified formulations

To confirm the moisturizing effect of red ginseng butter, lotion with no butter, lotion with shea butter and lotion with red ginseng butter was prepared. First, the water and soluble images were weighed into a container. The dissolvable image and the water phase dissolved in the solubilized portion were completely mixed and dissolved by dissolution in water-soluble dissolution and dissolvable image dissolution, and mixed and stirred. The additives were added to the mixed and stirred mixture and stirred uniformly. The content ratio of each lotion is shown in Tables 3 to 5 below.

<3-1> No added butter lotion - Control

Raw material name standard Mixing ratio
(%) P-water ICID To 100 Elaeis Guineensis (Palm) oil / Elaeis Guineensis (Palm) Kernel Oil ICID 5.0 Glyceryl Stearate ICID 1.0 Stearic acid ICID 1.0 Xanthan Gum ICID 0.2 Glycerin ICID 10.0 Vegetable Oil ICID 10.0 1,2-Hexadiol ICID 2.0 Sum - 100.00

<3-2> Lotion with red ginseng butter

Raw material name standard Mixing ratio
(%) P-water ICID To 100 Elaeis Guineensis (Palm) oil / Elaeis Guineensis (Palm) Kernel Oil ICID 5.0 Glyceryl Stearate ICID 1.0 Stearic acid ICID 1.0 Xanthan Gum ICID 0.2 Glycerin ICID 10.0 Ginseng Butter ICID 5.0 Vegetable Oil ICID 10.0 1,2-Hexadiol ICID 2.0 Sum - 100.00

<3-3> SHARE  Butter added lotion

Raw material name standard Mixing ratio
(%) P-water ICID To 100 Elaeis Guineensis (Palm) oil / Elaeis Guineensis (Palm) Kernel Oil ICID 5.0 Glyceryl Stearate ICID 1.0 Stearic acid ICID 1.0 Xanthan Gum ICID 0.2 Glycerin ICID 10.0 Butyrospermum Parkii (Shea) Butter ICID 5.0 Vegetable Oil ICID 10.0 1,2-Hexadiol ICID 2.0 Sum - 100.00

The properties of the lotion prepared in Preparation Example 3 are shown in Table 6 below.

Production example 3-1 Production example 3-2 Production Example 3-3 pH 7.45 6.38 7.68 Viscosity (0 day) 6,600 7,300 6,900 Viscosity (1 day) 17,600 30,190 12,100 Emulsified particles
Visual confirmation result Good Good Good

As a result of comparing the properties of the lotion produced, the pH was lowered by the acidic polysaccharide contained in red ginseng, but was within the normal pH range of the cosmetic (pH 5.5 to 8). In general cosmetics, the thickening agent is used to increase the viscosity. Since the red ginseng butter maintains a sufficiently high viscosity by itself, an emulsified cosmetic formulation can be produced more easily than the shea butter.

< Example  3> Manufacturing example  Check for the presence of heavy metals in red ginseng 2

<3-1> Confirmation of lead, cadmium and arsenic content

 Samples were prepared to confirm the content of lead, cadmium and arsenic. Red ginseng butter (an amount corresponding to 5 to 20 g as a dried product) was transferred to a crucible, dried and carbonized, and then, it was converted at 450 ° C. After painting, ash with water, add 2 ~ 4 ml of hydrochloric acid and dry it in a drying apparatus. Add 1 ~ 2 ml of hydrochloric acid and dissolve by heating. If insoluble matter is present, it is filtered with asbestos or glass filter and used as test solution .

The content of lead in the sample was measured by atomic absorption spectrophotometry. The test solution containing the blank test solution and the red ginseng butter was intact or diluted or concentrated and then injected into an atomic absorption spectrophotometer to determine the absorbance. The standard solutions for lead, cadmium and arsenic and their blank solutions were also tested To prepare a calibration curve, and the concentration of the test solution was determined.

The amount of the identified heavy metals is shown in Table 7 below.

Pb CD As Measured value (mg / kg) Non-detection Non-detection Non-detection Limit of food poisoning limit (mg / kg) 20 5 10

It was confirmed that the heavy metals Pb, Cd and As were not detected in the red ginseng butter according to the present invention.

<3-2> Confirm mercury content

The test solution was prepared by sulfuric acid - nitric acid reflux method to confirm the content of mercury in red ginseng butter. Red ginseng butter (an amount equivalent to 5 to 10 g as dried product) of Preparation Example 2 was transferred to a decomposition flask, mixed with 10 ml of water and 20 ml of nitric acid, left to stand, and then 20 ml of sulfuric acid was slowly added. A reflux condenser was connected and heated until the evolution of NO ₂ was complete. After being decomposed liquid is transparent to light yellow and cooled end of the generation of NO _2, by placing a water ㎖ 50 and 10% urea solution 10 ㎖ cooled and heated for 10 minutes into a 1 g of potassium permanganate was mixed occasionally to shake for 10 minutes. When the magenta color disappears, 1 g of potassium permanganate is added and shaken. This process was repeated until the magenta color remained and the mixture was boiled for 20 minutes. When the magenta color of the solution disappeared, the mixture was cooled and then 1 g of potassium permanganate was added thereto, followed by heating for 20 minutes. If the magenta color of the solution disappears, add potassium permanganate and repeat the heating operation twice again, and carefully add 10% hydrogen peroxide solution until the solution becomes colorless and transparent. However, in case of colorimetry, 20% hydrochloric acid hydroxylamine solution is used instead of hydrogen peroxide. After cooling, the inside and connecting portions of the apparatus were washed with 20 ml of sulfuric acid and added to the flask to obtain a test solution.

Mercury was quantified by atomic absorption spectrophotometry (reduction vaporization). The absorbance of the test solution was measured using a reducing gasifier. The reagents and reagents used in the measurement are as follows. A solution of tin chloride (SnCl ₂ (II)) was prepared by dissolving 100 g of tin chloride in 100 ml of hydrochloric acid and adding water to make 1,000 ml. The standard solution of mercury was prepared by dissolving 0.135 g of mercuric chloride in 100 ml of 10% nitric acid and adding water to make 1,000 ml. The standard solution was diluted 1,000 times with 1% nitric acid. One milliliter of mercury standard solution contains 0.1 μl Hg.

Test solution, blank test solution and tin chloride solution were transferred to a test solution bottle, connected to a reducing gasifier, and air was circulated through the absorbing cell by a diaphram pump to measure the absorbance at a wavelength of 253.7 nm. Water was added to 0, 5, 10, 15, and 20 ml of mercury standard solution to make 100 ml, and the same procedure as in Test Solution was performed. The absorbance of each solution was measured and the content of mercury (Hg) was obtained from the calibration curve.

As a result of the confirmation, mercury was not detected in the red ginseng butter produced in Production Example 2 of the present invention.

<3-3> Confirmation of antimony content

The content of antimony was confirmed by the antimony analysis method disclosed in "Guideline for Analysis of Hazardous Substances in Cosmetics" (Food and Drug Administration). The analytical method is as follows.

First, a sample solution was prepared. About 0.2 g of red ginseng butter of Preparation Example 2 was weighed and placed in a container for microwave digestion of Teflon. To dissolve the specimen, 7 ml of nitric acid and 2 ml of hydrofluoric acid were added and the lid was closed. Then, the container was mounted in a microwave decomposition apparatus and the color was changed from colorless to pale yellow depending on the maximum power: 1000 W, the maximum temperature: 200 ° C., Lt; / RTI &gt; After cooling to room temperature, the lid was opened and 20 ml of diluted boric acid was added. The lid was closed, and the container was again mounted in a microwave breaking apparatus. The flask was immersed in fluorine at a maximum power of 1000 W, a maximum temperature of 180 ° C., Lt; / RTI &gt; After cooling to room temperature, the degradation product was transferred to a 100 ml volumetric flask, and the container and the lid were washed with an appropriate amount of water, and water was added to make 100 ml. The solution was diluted 5-fold with water to prepare a sample solution. Separately, 7 ml of nitric acid and 2 ml of hydrofluoric acid were treated in the same manner as the sample solution to prepare a blank test solution.

Diluted nitric acid was added to the antimony standard stock solution (1000 μg / ml) to prepare three or more standard solutions for the calibration curve having different concentrations. The concentration of this standard solution was in the range of 1 to 20 ng of antimony per 1 ml.

Each standard solution was injected into an inductively coupled plasma-mass spectrometer (ICP-MS) according to the following operating conditions and the amount of antimony in the sample solution was measured with the calibration curve of antimony obtained. The operating conditions are as follows.

Atomic Quantity: 121, 123 (detected by selecting from a range without interference)

Plasma gas: argon (99.99 v / v% or more).

As a result, 0.7 mg / kg of antimony was detected. It was somewhat lower than the limit of 10 mg / kg,

<3-4> Dioxane  Content check

Approximately 1.0 g of the red ginseng butter of Preparation Example 2 was weighed, and 1.0 ml of a 20% sodium sulfate solution was added and mixed to prepare a sample solution. Separately, 1,4-dioxane standards were diluted with water to prepare solutions having concentrations of 0.0125, 0.025, 0.05, 0.1, 0.2, 0.4 and 0.8 mg / ml. To each 50.0 μl were added about 1.0 g of polyethylene glycol 400 and 20 g of 20 Sodium perchlorate solution (1.0 ml) was added and the mixture was used as a standard solution. The test solution and the standard solution were tested according to the absolute calibration curve method of the gas chromatograph under the following conditions.

<Operating Conditions>

Detector: Mass Spectrometer

- Interface temperature: 240 ℃

- Ion source temperature: 230 ℃

- Scanning range: 40 ~ 200 amu

- Mass analyzer mode: Select ion mode (88, 58, 43)

Headspace

- Amount of injection (loop): 1 ml

- Vial Equilibration Temperature: 95 ° C

- Loop temperature: 110 ℃

- Infusion line temperature: 120 ° C

- Vial purge pressure: 20 psi

- Vial equilibration time: 30 minutes

- Vial purge time: 0.5 minutes

- Loop filling time: 0.3 minutes

- Loop equilibration time: 0.05 minutes

- Injection time: 1 minute

Column: A tube having an inner diameter of about 0.32 mm and a length of about 60 mm was coated with a 500 탆 silane-treated polyethylene terephthalate wax for diatomaceous earth gas chromatograph.

Column temperature: maintained at 50 占 폚 for the first 2 minutes and increased by 10 占 폚 in 1 minute to 160 占 폚.

Carrier gas: helium

Flow rate: Adjust so that the retention time of 1,4-dioxane is about 10 minutes.

Split ratio: about 1:10

As a result, the amount of dioxane was detected to be 14.60 mg / kg, which was somewhat lower than that of 100 mg / kg,

<3-5> Determination of formaldehyde content

About 1.0 g of red ginseng butter of Preparation Example 2 is weighed and 20 ml of acetic acid-sodium acetate buffer solution is added and shaken for 1 hour. 1 ml of this solution is diluted to 200 ml with water, and 100 ml of this solution is diluted with acetic acid-sodium acetate buffer 4 ml was added, and homogeneously mixed. The pH was adjusted to 5.0 by adding 6 mol / l hydrochloric acid or 6 mol / l sodium hydroxide solution. To this solution, 6.0 ml of 2,4-dinitrophenylhydrazine citrate was added and the mixture was shaken at 40 ° C for 1 hour. The mixture was extracted three times with 20 ml of dichloromethane and then filtered through a funnel containing 5.0 g of anhydrous sodium sulfate. The filtrate was evaporated to dryness under reduced pressure, and 5.0 ml of acetonitrile was added to the residue. The solution was used as the sample solution. Separately, formaldehyde standards were diluted with water to make 0.001, 0.01, 0.03, 0.05, 0.1 and 0.2 μg / ml, and 100 ml of each solution was pretreated in the same manner as the sample solution. Each 10 μl of the test solution and standard solution was tested according to the absolute calibration method of the liquid chromatography method under the following conditions.

<Operating Conditions>

Detector: Ultraviolet absorptiometer (measuring wavelength 355 nm)

Column: Stainless steel pipe with an inner diameter of about 4.6 mm and a length of about 25 cm.

Octadecylsilylated silica gel for a liquid chromatograph was filled.

Mobile phase: 0.01 mol / l hydrochloric acid · acetonitrile mixture (40:60)

Flow rate: 1.5 ml / min

As a result of measurement, formaldehyde was not detected.

< Experimental Example  1> Manufacturing example  3 of the emulsified formulation Moisture power  evaluation

The test area of 2 cm in diameter was marked on the skin of the subject and allowed to stand in a constant temperature and humidity room (25 ° C, 50% humidity) for about 30 minutes. The initial water content of the skin was measured using Corneometer® CM825 (Courage + Khazaka Instruments). 10 to 15 占 퐂 of the emulsified formulations of Production Examples 3-1 to 3-3 were applied to the affected area, and after 30 minutes to 4 hours, they were allowed to stand in a constant temperature and humidity room for about 30 minutes and then the water content of the same area was measured. The measured value was calculated by the formula: (water content after application - initial water content) / (initial water content) to calculate the water increase amount.

As a result, the emulsified formulation containing 3% of red ginseng butter and the emulsified formulation containing 3% of shea butter showed a moisturizing effect of 59.1% and 47.7%, respectively, as compared with the control formulation of Comparative Example 3-1 containing no butter, The emulsion formulation containing 3% of red ginseng butter showed about 11.4% higher moisturizing effect than the emulsion formulation containing 3% of shea butter. It was confirmed that red ginseng butter showed a moisturizing effect suitable for use in cosmetics.

< Experimental Example  2> Antioxidative efficacy evaluation

Each butter was dissolved in ethanol at a concentration of 0.1%, and the DPPH reagent (5.9 mg of DPPH diluted in ethanol) was mixed with 1: 1. After incubation at room temperature for 30 minutes, the absorbance at 517 nm was measured. Respectively. The DPPH radical scavenging capacity (%) was determined from the equation 100 - [(absorbance of the reaction group to which the sample was added / absorbance of the control group to which no sample was added) × 100]. As a positive control, ascorbic acid, known as an antioxidant, was used.

The results are shown in Table 8 below.

Red ginseng butter
0.1% Cocoa butter
0.1% Mururu
0.1% Mango butter
0.1% Cucum butter
0.1% Sheer butter
0.1% Ascorbic acid
0.01% Ascorbic acid 0.001% 0.342 0.421 0.422 0.409 0.418 0.467 0.211 0.272 0.351 0.437 0.425 0.413 0.408 0.448 0.213 0.265 0.37 0.441 0.411 0.423 0.407 0.412 0.212 0.261 Average 0.3543 0.4330 0.4193 0.4150 0.4110 0.4423 0.2120 0.2660 Antioxidative effect 34.6% 3.7% 9.0% 10.7% 12.3% 0.0% 90.7% 69.4%

As shown in Table 8, when the red ginseng butter was contained, it was confirmed that the antioxidative effect was maintained even with the hydrogenation reaction, and the antioxidative effect was higher than that of the other plant-derived butter.

Hereinafter, the present invention will be described in detail with reference to Preparation Examples.

However, the following Production Examples are only for illustrating the present invention, and the content of the present invention is not limited by the following Production Examples.

< Manufacturing example  4> Cosmetics  Preparation of composition

<4-1> Preparation of essence

Using the red ginseng butter of Example 2, essences were prepared according to the contents (parts by weight) shown in Table 9 below.

Furtherance Content (parts by weight) The red ginseng butter of Example 2 3 Triethanolamine 0.25 Carboxyvinyl polymer 0.22 glycerin 4 Butylene glycol 2 Paraoxybenzoic acid ester 0.2 Wax 0.5 Cetostearyl alcohol One Glyceryl monostearate One Squalene 4 Purified water Suitable amount

<4-2> Production of softening longevity

The softening water containing the red ginseng butter of Example 2 of the present invention as an active ingredient was prepared as shown in Table 10 below.

Raw material Content (parts by weight) The red ginseng butter of Example 2 0.1 1,3-butylene glycol 1.00 Disodium iodide 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric acid 0.01 Sodium citrate 0.02 Glycereth-26 1.00 Arbutin 2.00 PEG-40
Hydrogenated castor oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

<4-3> Preparation of nutritional cream

The nutritional cream containing the red ginseng butter of Example 2 of the present invention as an active ingredient was prepared according to the composition shown in Table 11 below.

Raw material Content (parts by weight) The red ginseng butter of Example 2 1.0 1,3-butylene glycol 7.0 glycerin 1.0 D-Panthenol 0.1 Plant extract 3.2 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl alcohol 3.0 Mineral oil 4.0 Squalene 3.8 Carlyric / capric triglyceride 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Suitable amount Triethanolamine Suitable amount Preservative Suitable amount Flavoring agent Suitable amount Purified water Balance

<4-4> Production of Lotion

Lotions containing the red ginseng butter of Example 2 of the present invention as an active ingredient were prepared as shown in Table 12 below.

Raw material content( Weight portion ) Cetostearyl alcohol 1.6 Stearic acid 1.4 Pro-type glycerin monostearate 1.8 PEG-100 stearate 2.6 Sorbic acid sesquioleate 0.6 Squalene 4.8 Macadia oil 2 Jojoba oil 2 Tocopheryl acetate 0.4 Methylpolysiloxane 0.2 Ethyl paraben 0.1 Tocopheryl acetate 0.4 Methylpolysiloxane 0.2 Ethyl paraben 0.1 Propylparaben 0.1 1,3-butylene glycol 4 Methyl paraben 0.1 Xanthan gum 0.1 glycerin 4 d-Pandenol 0.15 Allantoin 0.1 The red ginseng butter of Example 2 1.0 Carbomer (2% aq. Sol) 4 Triethanolamine 0.15 ethanol 3 pt 41891 0.1 p-H20 48.3

Claims (10)

A cosmetic composition for moisturizing or antioxidation containing red ginseng butter as an active ingredient,
The red ginseng butter
1) extracting red ginseng oil with supercritical extraction using carbon dioxide;
2) purifying the extracted red ginseng oil by fractional distillation; And
3) curing the purified red ginseng oil. delete delete delete delete The method according to claim 1,
Wherein the red ginseng oil purified in step 2) is selected from the group consisting of C14: 0 myristoleic acid, C16: 0 palmitic acid, C16: 1 palmitoleic acid, C18: 0 stearic acid, C18: 1 oleic acid, C18: Linolenic acid, and C20: 0 arachidic acid. The method of claim 6,
The ratio of each fatty acid to 100 parts by weight of the fat is 0.05 to 5 parts by weight of C14: 0 mistelliteoleic acid, 10 to 30 parts by weight of C16: 0 palmitic acid, 0.05 to 5 parts by weight of C16: 1 palmitoleic acid 0.01 to 5 parts by weight of the C18: 0 stearic acid, 1 to 10 parts by weight of the C18: 1 oleic acid, 30 to 80 parts by weight of the C18: 2 linoleic acid, 0.1 to 5 parts by weight of the C18: 3 linolenic acid, : 0 &lt; / RTI &gt; arachidic acid in an amount of 0.05 to 5 parts by weight. In claim 1,
Wherein the red ginseng oil is cured by a hydrogenation reaction using nickel as a catalyst in the step 3). The method according to claim 1,
Wherein the red ginseng butter is contained in an amount of 0.1 to 20 parts by weight based on 100 parts by weight of the composition. The method according to claim 1,
Wherein the composition has a formulation of a softening agent, a nutritional lotion, a nutritional cream, a massage cream, an essence, a cleansing cream, a cleansing foam, a pack, a body oil or a powder.