KR102039094B1 - Composition for Improving Skin Hydration Using Natural Plant Extract or Monosaccharide - Google Patents

Abstract

The present invention relates to a skin moisturizing composition, and more particularly, at least two selected from the group consisting of rhododendron extract, white pattern thistle extract, european horse riding extract, glucose, galactose, fucose and N-acetylglucosamine It relates to a skin moisturizing cosmetic composition and a pharmaceutical composition containing as a component. The composition of the present invention increases the moisture content of the skin through a single application to normal skin and diseased skin in a short time, thereby making it useful for the development of cosmetic compositions or pharmaceutical compositions that can improve dry skin through skin moisturizing. Can be used.

Description

Composition for Improving Skin Hydration Using Natural Plant Extract or Monosaccharide}

The present invention relates to a composition for improving skin moisturizing, and more particularly, to a cosmetic composition or a pharmaceutical composition capable of improving dry skin symptoms through skin moisturizing by increasing moisture content by applying to normal skin and diseased skin.

The moisture level of the skin is one of the important indicators of the physiological state of the skin. The higher the moisture level, the more healthy and moist skin, and the lower the moisture level, the dry and damaged skin. In general, the moisture content of the skin is the amount of water evaporated per hour (transdermal moisture loss) on the surface of the skin according to the moisture and barrier function, which measure the moisture content of the skin surface by an electrical method using a coronometer. It can be evaluated quantitatively by measuring the transdermal moisture loss by using a transdermal moisture loss measuring instrument (Tewameter), and the moisture content is evaluated to be better as the numerical value is increased and the transdermal moisture loss is decreased.

Molecular mechanisms affecting water content and transdermal moisture loss are diverse and different, so the two factors must be adjusted basically, but the two factors are related and indirectly affect. In general, moisturizers containing lipids such as ceramide are used to supplement the skin barrier to prevent transdermal moisture loss, thereby moisturizing effect, but the mechanism of increasing the natural moisturizing factor is to increase the moisture content. The mechanism of moisturizing can be seen.

The search for moisturizing ingredients can be screened through mechanism studies, but it is not difficult to measure the moisturizing effect directly by applying the human body. Therefore, the application of natural extracts or lipid components to increase moisture content or transdermal moisture Components that reduce or recover loss can be screened.

On the other hand, as a substance for increasing skin moisture, using jasmine, arnica, yarrow extract (patent document 1), aloe vera, marshmallow extract (patent document 2), branch, golden, janggeun, white paper, green beans Attempts have been made to use various kinds of natural substances, such as using complex herbal extracts using licorice, algae, and the like (Patent Document 3) or using red ginseng oil or red ginseng butter (Patent Documents 4 and 5). However, although there are many previous studies using natural extracts as described above, natural extracts themselves have limitations in maintaining moisturizing properties, and there are few previous studies on skin moisturizing improvement effects using saccharide components.

Accordingly, the present inventors have a combination of natural monosaccharide extracts, such as ryejang root extract, white thistle extract, European horseback riding extract, and four monosaccharides of glucose, galactose, fucose, N-acetylglucosamine, and the like. The present invention has been completed by revealing that there is a significant increase in skin moisture.

Republic of Korea Patent Publication No. 10-2003-0085343 Republic of Korea Patent Publication No. 10-2010-0087522 Republic of Korea Patent Publication No. 10-2014-0126901 Republic of Korea Patent Publication No. 10-2014-0000882 Republic of Korea Patent Publication No. 10-2015-0071858

The present invention is to provide a composition that is safe for the human body and exhibits superior skin moisturizing properties, and provides a composition for improving skin moisturization, which includes effective ingredients that increase moisture content when a combination of natural extracts and monosaccharides is applied to human skin. It is technical problem to do.

In order to achieve the above object, the present invention is a skin containing two or more selected from the group consisting of rhododendron extract, white thistle extract, European horse riding extract, glucose, galactose, fucose and N-acetylglucosamine as an active ingredient It provides a moisturizing composition. According to a preferred embodiment of the present invention, the skin moisturizing composition of the present invention is preferably in the form of a cosmetic composition or a pharmaceutical composition, but is not limited thereto.

According to one embodiment of the invention, the composition comprises (1) a mixture of glucose, galactose, fucose and N-acetylglucosamine, (2) a mixture of glucose, fucose and N-acetylglucosamine, (3) glucose and N A mixture of acetylglucosamine, (4) a mixture of rhododendron extract, white thistle extract and european horse riding extract, (5) a mixture of rhododendron extract, white thistle extract, european riding horse extract and glucose, and (6) rye root extract , White patterned thistle extract, European horseback extract, mixture of glucose and N-acetylglucosamine, and (7) rye root extract, white patterned thistle extract, European riding horse extract, glucose, fucose and mixture of N-acetylglucosamine It may contain an active ingredient selected from, but is not limited thereto.

According to a preferred embodiment of the invention, (1) the mixture of glucose, galactose, fucose and N-acetylglucosamine is glucose: galactose: fucose: N-acetylglucosamine = 1: 0.5-2: 0.5-2: 0.5 (2) the mixture of glucose, fucose and N-acetylglucosamine is mixed in a weight ratio of glucose: fucose: N-acetylglucosamine = 2: 0.5-2: 1-4, 3) The mixture of glucose and N-acetylglucosamine is mixed in a weight ratio of glucose: N-acetylglucosamine = 1: 0.5 to 2, and (4) the mixture of the rhododendron extract, white pattern thistle extract and the European horseback riding extract is Knotweed Extract: White Patterned Thistle Extract: European Horseriding Extract = 1: 0.5 to 2: 0.5 to 2, and mixed in a weight ratio of (5) The Kwonjang Extract, White Patterned Thistle Extract, European Horseriding Extract and Glucose Mixture Extract: White Milk Thistle Extract: European Equestrian Extract: Glucose = 2: 1 to 4: 1 to 4: 2 to 8, and mixed at a weight ratio of (6) said K. Root Extract, White Patterned Thistle Extract, European Equestrian Extract, Glucose and N- The mixture of acetylglucosamine is mixed in a weight ratio of rhododendron extract: white pattern thistle extract: European horse riding extract: glucose: N-acetylglucosamine = 2: 1 to 4: 1 to 4: 1 to 5: 1 to 5, (7 A) The mixture of rhododendron extract, white thistle extract, European horseback extract, glucose, fucose and N-acetylglucosamine may include rhododendron extract: white patterned thistle extract: European horseriding extract: glucose: fucose: N-acetylglucosamine = It may be mixed in a weight ratio of 2: 1 to 4: 1 to 4: 1 to 4: 0.5 to 2: 1 to 4, but is not limited thereto.

According to a preferred embodiment of the present invention, the extract may contain but is not limited to resveratrol.

According to one embodiment of the invention, the active ingredient may be contained in an amount of 0.001 to 10% by weight in the total composition, but is not limited thereto.

According to one embodiment of the invention, the composition may have a skin external preparation formulation, the formulation is a softening longevity, nourishing longevity, massage cream, nutrition cream, pack, gel, lotion, ointment, gel, cream, patch and spray It may be selected from the group consisting of, but is not limited thereto.

According to one embodiment of the invention, the composition can be used for moisturizing the skin in diseases associated with a decrease in skin moisture. According to a preferred embodiment of the present invention, the disease may be selected from the group consisting of dry skin, eczema, psoriasis, atopic dermatitis, young and celestial spear, but not limited thereto.

According to one embodiment of the invention, the composition may be in the form of a parenteral dosage form selected from the group consisting of solid, semisolid and liquid, but is not limited thereto.

The composition of the present invention increases the moisture content of the skin through a single application to normal skin and diseased skin in a short time, thereby making it useful for the development of cosmetic compositions or pharmaceutical compositions that can improve dry skin through skin moisturizing. Can be used.

1 is a graph showing the water content increase effect of resveratrol-containing liquid, white pattern thistle extract and European horse riding extract derived from the extract of Kwon Keun-Geun.
Figure 2 is a mixture of four monosaccharides of glucose, galactose, fucose and N-acetylglucosamine (4-Mix), a mixture of two monosaccharides of glucose and N-acetylglucosamine (GN-Mix), resveratrol derived from e.g. Aqueous solution, white thistle extract, European horse riding extract, a mixture of glucose (Hoong riding G-mix), a resveratrol containing liquid derived from rhododendron extract, white thistle extract, a European riding horse extract, glucose, a mixture of N-acetylglucosamine ( Resveratrol-containing liquid derived from hawthorn GN-mix) and rhododendron extract, white thistle extract, European horse riding extract, glucose, fucose, and N-acetylglucosamine (Hungseung GFN-mix) It is a graph.

In order to achieve the above object, the present invention is moisturizing skin containing two or more components selected from the group consisting of rhododendron extract, white thistle extract, European horse riding extract, glucose, galactose, fucose and N-acetylglucosamine To provide a composition. It is preferable that the extract of Keunjangeun contains resveratrol as an active ingredient.

According to one embodiment of the invention, a mixture of four monosaccharides of glucose, galactose, fucose and N-acetylglucosamine (hereinafter referred to as '4-Mix') is used as a skin moisturizing composition. According to another embodiment of the present invention, a mixture of glucose, fucose and N-acetylglucosamine (hereinafter referred to as 'GFN-Mix') is used as a skin moisturizing composition. According to another embodiment of the present invention, a mixture of glucose and N-acetylglucosamine (hereinafter referred to as 'GN-Mix') is used as a skin moisturizing composition. According to another embodiment of the present invention, a mixture of rhododendron extract, white thistle extract, European horseback riding extract, and GFN-Mix (hereinafter, referred to as 'hugung GFN-Mix') is used as a skin moisturizing composition. According to another embodiment of the present invention, a mixture of rhododendron extract, white patterned thistle extract, European horseback riding extract, and GN-Mix (hereinafter, referred to as 'Engungseung GN-Mix') is used as a skin moisturizing composition. According to another embodiment of the present invention, a mixture of rhododendron extract, white patterned thistle extract, European horseback riding extract and glucose (hereinafter referred to as 'Engungseung G-Mix') is used as a skin moisturizing composition. According to another embodiment of the present invention, a mixture of rhododendron extract, white patterned thistle extract, and European horseback riding extract (hereinafter referred to as 'horned-Mix') is used as a moisturizing composition for skin. According to a preferred embodiment of the present invention, it is preferable that the extract of Kwon Keun-Geun contains resveratrol as an active ingredient.

According to one embodiment of the invention, the 4-Mix may be mixed, for example, in a weight ratio of glucose: galactose: fucose: N-acetylglucosamine = 1: 0.5-2: 0.5-2: 0.5-2, and 1: It is more preferable to mix in a weight ratio of 1: 1: 1, but the ratio of each component which comprises the said mixture may be somewhat different from the above. According to another embodiment of the present invention, the GFN-Mix may be mixed, for example, in a weight ratio of glucose: fucose: N-acetylglucosamine = 2: 0.5 to 2: 1 to 4, and in a weight ratio of 2: 1: 2. Although it is more preferable to mix, the ratio of each component which comprises the said mixture may be somewhat different from the above. According to another embodiment of the present invention, the GN-Mix may be mixed, for example, in a weight ratio of glucose: N-acetylglucosamine = 1: 0.5 to 2, more preferably in a weight ratio of 1: 1, but the mixture The ratio of each component which comprises can be somewhat different from the above. According to another embodiment of the present invention, the whittail-Mix may be mixed at a weight ratio of, for example, rhododendron extract: white patterned thistle extract: European horse riding extract = 1: 0.5-2: 0.5-2, and 1: 1: Although it is more preferable to mix in the weight ratio of 1, the ratio of each component which comprises the said mixture may be somewhat different from the above. According to another embodiment of the present invention, the H. horn G-Mix may be mixed at a weight ratio of, for example, Rhodon root extract: White patterned thistle extract: European horse riding extract: Glucose = 2: 1 to 4: 1 to 4: 2 to 8 Although it is more preferable to mix in the weight ratio of 2: 2: 2: 5, the ratio of each component which comprises the said mixture may be somewhat different from the above. According to another embodiment of the present invention, the Horned GN-Mix may be, for example, Rhodon root extract: White patterned thistle extract: European horse riding extract: Glucose: N-acetylglucosamine = 2: 1 to 4: 1 to 4: 1 to 5 It may be mixed at a weight ratio of 1 to 5, and more preferably at a weight ratio of 2: 2: 2: 2.5: 2.5, but the ratio of each component constituting the mixture may be slightly different from the above. . According to another embodiment of the present invention, the horned GFN-Mix may include, for example, rhododendron extract: white patterned thistle extract: European horse riding extract: glucose: fucose: N-acetylglucosamine = 2: 1 to 4: 1 to 4: It can be mixed at a weight ratio of 1 to 4: 0.5 to 2: 1 to 4, and more preferably at a weight ratio of 2: 2: 2: 2: 2: 1: 2, but each of the components constituting the mixture The ratio may be slightly different from the above. In addition, it is preferable that the extract of Kwon Keun-Geun contains resveratrol as an active ingredient.

In the present invention, the natural extract or monosaccharide included in the composition of the present invention may be prepared by any one of the methods well known in the art, or may be easily purchased on the market. According to a preferred embodiment of the present invention, it is preferable that the extract of Keunjangeun contains resveratrol as an active ingredient, but is not limited thereto. According to one embodiment of the present invention, a resveratrol-containing liquid derived from other natural products other than Kwon Keun-Geun may be used, or a synthesized resveratrol-containing liquid may be used.

According to one embodiment of the present invention, the natural product extract is preferably extracted by adding an extraction solvent after mixing alone or two or more of individual natural products, for example, rye root, white thistle and European horseback riding. According to a preferred embodiment of the present invention, it is preferable to extract by adding 2 to 20 times (w / v), preferably 5 to 15 times (w / v) the extraction solvent of the dry weight of the natural product or mixture of natural products. . Although water is generally used as the extraction solvent, other organic solvents may be used in addition to water as necessary. Organic solvents that may be used include, but are not limited to, C ₁ to C ₄ lower alcohols, preferably ethanol, acetone, or aqueous solutions thereof. In the present invention, the "natural extract" is used as a meaning encompassing not only the natural extract prepared by using the extraction solvent, but also the fraction extracted by adding one or more "fractionated organic solvents" to the natural extract. The fractionated organic solvent is not particularly limited, and conventional organic solvents may be used without limitation.

In the present invention, the natural product extract can be extracted by room temperature or warming under the condition that the active ingredient is not destroyed or minimized. According to one embodiment of the invention, the natural product extract is (1) extracting by adding water or an organic solvent, such as ethanol solvent to the natural product or a mixture of natural products, (2) filtering the extract of step (1), And (3) may be prepared by a method comprising the step of powdering the filtered extract of step (2), but is not limited thereto.

In addition, water and organic solvents such as ethanol, preferably 50% ethanol, may be used to extract the water-soluble and non-water soluble components to extract various components of the natural product of step (1). According to one embodiment of the present invention, the natural product extract uses a volume of 50% ethanol solvent of about 2 to 20 times the dry weight of the natural product as the elution solvent, the extraction temperature is 20 to 100 ℃, preferably 22 to At a temperature of 50 ° C., more preferably 25 ° C., the extraction period is any time such as shaking extraction, hot water extraction, cold extraction, reflux cooling extraction or ultrasonic extraction for about 1 hour to 10 days, preferably 5 hours to 2 days. Can be made using the extraction method. According to another embodiment of the present invention, by calculating the water content contained in the raw material to add ethanol to the final concentration of 50%, it can be extracted for 5 minutes at 90 W in a microwave oven.

In the present invention, the two or more components selected from the group consisting of the rhododendron extract, white pattern thistle extract, European horse riding extract, glucose, galactose, fucose and N-acetylglucosamine are 0.001 to 10% by weight in the total composition, It is preferably contained in an amount of 0.005 to 8% by weight, more preferably 0.01 to 5% by weight. If it is less than 0.001% by weight, the effect of improving skin moisturization is insignificant, and if it exceeds 10% by weight, compatibility with other ingredients and formulation stability are poor. In this regard, two or more components selected from the group consisting of the extract of K. koji extract, white thistle extract, European horse riding extract, glucose, galactose, fucose and N-acetylglucosamine are present in an amount of 0.01 to 5% by weight in the total composition. It is more preferable to contain.

In the present invention, the improvement of skin moisture can be applied to normal skin and diseased skin, and when applied to diseased skin, it can be helpful in alleviating, reducing and treating the symptoms of the disease. According to one embodiment of the present invention, the disease may be affected by a decrease in skin moisture level, or any disease in which skin moisture level is reduced as a result of the disease may be included without limitation. According to a preferred embodiment of the present invention, the disease may include such as dry skin, eczema, psoriasis, atopic dermatitis, young glands, pemphigus, and the like, but is not limited thereto, for reasons such as excessive inflammation or barrier damage on the skin Any disease that causes discomfort such as pulling, itching or itching due to a decrease in skin moisture due to it may be included in the scope of the disease of the present invention.

The formulation of the composition of the present invention is not particularly limited, but is preferably a formulation of an external preparation for skin, and a preferred form of the external preparation for external preparation includes softening cream, nourishing cosmetic, massage cream, nourishing cream, pack, gel or skin adhesion. Cosmetic compositions having formulations of type cosmetics, and other preferred forms include formulations of transdermal dosage forms such as lotions, ointments, gels, creams, patches or sprays. In addition, in the external preparation composition of each formulation, other components than the essential components described above may be appropriately selected and blended by those skilled in the art without difficulty according to the formulation or purpose of use of other external preparations.

  According to a preferred embodiment of the present invention, the skin moisturizing composition of the present invention is preferably in the form of a cosmetic composition or a pharmaceutical composition, but is not limited thereto.

When the skin moisturizing composition of the present invention is formulated as a cosmetic composition, the component included in the cosmetic composition of the present invention is an active ingredient as described above as an active ingredient extract, white lice thistle extract, European horse riding extract, glucose, galactose, fuco In addition to the two or more components selected from the group consisting of os and N-acetylglucosamine may include components conventionally used in cosmetic compositions within the scope that does not impair the desired effect of the present invention, such as antioxidants, stable Conventional adjuvants and carriers such as agents, solubilizers, vitamins, pigments and flavorings.

The cosmetic composition according to the present invention is not particularly limited in its formulation, and may be prepared in any formulation conventionally prepared in the art, for example, softening cream, nutrient cream, essence, lotion, nourishing cream, It may be prepared in the form of a massage cream, a pack, a gel, an ointment, a patch, a spray, a linen, a pasta or a cataplasm. Here, when the formulation is an ointment, it is prepared to contain 50.0 to 97.0% by weight of petrolatum, and 0.1 to 5.0% by weight of polyoxyethylene oleyl-ether phosphate in addition to the active ingredients as described above, the softening longevity is propylene glycol, It is manufactured to contain 1.0-10.0 weight% of polyhydric alcohols, such as glycerin, and 0.05-2.0 weight% of surfactants, such as polyethylene oleyl ether and polyoxyethylene hardened castor oil. Nourishing cream and nourishing cream contains 5.0 to 20.0 wt% of oils such as squalane, petrolatum and octyldodecanol, and 3.0 to 15.0 wt% of wax components such as cetanol, stearyl alcohol and beeswax, and the essence is glycerin and propylene. 5.0-30.0 weight% of polyhydric alcohols, such as glycol, are contained. Massage cream is prepared by containing 30.0 to 70.0% by weight of oils such as liquid paraffin, petrolatum and isononyl isononanoate in addition to the active ingredient, and the pack is peeled off containing 5.0 to 20.0% by weight of polyvinyl alcohol. The pack or general emulsion cosmetic may be prepared as a wash off pack containing 5.0 to 30.0% by weight of pigments such as kaolin, talc, zinc oxide and titanium dioxide.

According to one embodiment of the invention, when the composition of the invention is applied to a cosmetic, it preferably contains a cosmetically acceptable medium or base. These are all formulations suitable for topical application, for example emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase, and / or It may be provided in the form of a nonionic vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray or cone stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. In addition, the cosmetic composition according to the present invention can be prepared in the form of topically or systemically applicable auxiliaries commonly used in the field of dermatology by containing a dermatologically acceptable medium or base, and these compositions are known in the art. It may be prepared according to the conventional method of.

When the composition of the present invention is applied to cosmetics, the cosmetic composition is a fatty substance, an organic solvent, a dissolving agent, a thickening agent and a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ion Any commonly used in type or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics It may contain adjuvants conventionally used in the cosmetic field, such as other ingredients. These adjuvants are introduced in amounts generally used in the cosmetic field.

In addition, according to another preferred embodiment of the present invention, the skin moisturizing composition of the present invention may be prepared in the form of a pharmaceutical composition for moisturizing the skin in diseases associated with a decrease in skin moisture.

According to one embodiment of the present invention, when the improvement of skin moisture level is applied to the diseased skin, it may help to alleviate, alleviate, and treat the symptoms of the disease. The disease can be included without limitation any disease that is affected by a decrease in skin moisture or that reduces skin moisture as a result of the disease. According to a preferred embodiment of the present invention, the disease may include such as dry skin, eczema, psoriasis, atopic dermatitis, young glands, pemphigus, and the like, but is not limited thereto, for reasons such as excessive inflammation or barrier damage on the skin Any disease that causes discomfort, such as pulling, itching or itching due to a decrease in skin moisture due to it, may all be included in the scope of the disease to be treated of the composition of the present invention.

The content of two or more active ingredients selected from the group consisting of rhododendron extract, white patterned thistle extract, European horseback riding extract, glucose, galactose, fucose and N-acetylglucosamine contained in the pharmaceutical composition is the total weight of the pharmaceutical composition. It is preferably 0.001 to 10% by weight, more preferably 0.01 to 5% by weight, but is not limited thereto. If the content of the active ingredient is less than 0.001% by weight, the skin moisturizing effect cannot be obtained, and if the content of the active ingredient exceeds 10% by weight, there is a problem in that it is not economical because more active ingredients are included.

Routes of administration of the pharmaceutical compositions according to the invention include oral, intravenous, intramuscular, intraarterial, transdermal, subcutaneous, intraperitoneal, intranasal, intestinal, topical, sublingual or rectal, and parenteral administration may also be selected. And more preferably applied in a topical application manner by application. The parenteral includes subcutaneous, intradermal, intravenous, intramuscular, intralesional injection or infusion techniques. Preparations for parenteral administration include, but are not limited to, topical injections, ointments, lotions, sprays, suspensions, and the like. According to one embodiment of the invention, the pharmaceutical composition may be in the form of a parenteral dosage form selected from the group consisting of solid, semisolid and liquid, but is not limited thereto.

The pharmaceutical composition of the present invention may further contain one or more components exhibiting the same or similar functions in addition to the active ingredient. Each of the compositions can be used in the form of oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, external preparations, suppositories, and sterile injectable solutions according to conventional methods known in the art. have.

Solid preparations for oral administration include powders, granules, tablets, hard capsules, soft capsules, pills and the like. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include powders, granules, tablets, capsules, sterile aqueous solutions, solutions, non-aqueous solutions, suspensions, emulsions, syrups, suppositories, aerosols, etc. It may be used in the form of a formulation, and preferably, an external skin pharmaceutical composition of a cream, a gel, a patch, a spray, an ointment, a warning, a lotion, a linen, a pasta, or a cataplasm may be prepared. It is not limited to this. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The pharmaceutical composition may further contain preservatives, stabilizers, hydrating or emulsifying accelerators, auxiliaries such as salts and / or buffers for the control of osmotic pressure, and other therapeutically useful substances, and conventional methods of mixing, granulating It may be formulated according to the formulation or coating method. The pharmaceutical compositions according to the invention may vary depending on several factors, including the age, body weight, general health, sex, time of administration, route of administration, rate of release, drug combination and severity of the particular disease of the individual.

Suitable dosages of the pharmaceutical compositions of the present invention may be variously prescribed by such factors as formulation method, mode of administration, age, weight, sex, morbidity, condition of food, time of administration, route of administration, rate of excretion and response to response of the patient. Can be. Dosage determination based on these factors is within the level of the person skilled in the art, and for formulations other than injections, it can be applied at the level of the person skilled in the art by external application to topical sites, Injectables may be locally injected into the dermis and subcutaneous fat, but are not necessarily limited thereto. In general, the dosages of the pharmaceutical compositions according to the invention range from approximately 0.001 mg / kg / day to 2,000 mg / kg / day. More preferred dosages are 1 μg / kg / day to 100 μg / kg / day. Dosage units may, for example, contain 1, 2, 3 or 4 times the individual dose, or 1/2, 1/3 or 1/4 times. Individual dosages contain amounts in which the active ingredient is administered at one time, which usually corresponds to all, 1/2, 1/3 or 1/4 times the daily dosage.

The pharmaceutical compositions according to the invention can be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy and biological response modifiers.

In addition, according to another preferred embodiment of the present invention, the skin moisturizing composition of the present invention may be prepared in the form of health food.

The content of two or more active ingredients selected from the group consisting of rhododendron extract, white pattern thistle extract, european horse riding extract, glucose, galactose, fucose and N-acetylglucosamine contained in the health food may be appropriately used according to the purpose of use. Can be determined. It is preferable that the extract of Keunjangeun contains resveratrol as an active ingredient. According to one embodiment of the invention, the content of the active ingredient contained in the health food is preferably 0.001 to 10% by weight, more preferably 0.01 to 5% by weight based on the total weight of the health food, but is not limited thereto. no. If the content of the active ingredient is less than 0.001% by weight, the skin moisturizing effect is not obtained, and if the content exceeds 10% by weight, it is not economical to use more than necessary amount and there is a problem that the stability of the composition is lowered. However, in the case of long-term intake for health and hygiene or health control purposes, the content of the active ingredient may be less than the above range, and there is no problem in terms of safety. May also be used.

When the composition of the present invention is prepared as a health food, it may include ingredients that are commonly added during food preparation, and may include, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavoring agents. Examples of such carbohydrates are monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And sugars such as conventional sugars such as polysaccharides such as dextrin, cyclodextrin and the like and xylitol, sorbitol, erythritol. As the flavoring agent, natural flavoring agent taumartin, stevia extract and synthetic flavoring agent may be used. For example, when the health food of the present invention is prepared with a drink, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, natural extracts, etc. may be further included in addition to the active ingredient.

There is no particular limitation on the kind of the health food. Examples of foods to which the active ingredient can be added according to the present invention include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including gums, ice cream, various soups, beverages , Teas, drinks, alcoholic beverages and vitamin complexes and the like, and includes all of the health foods in the conventional sense.

According to a preferred embodiment of the present invention, a composition containing 2% of resveratrol-containing liquid derived from Rhodon root extract, 2% of white patterned thistle extract or 2% of European horse riding extract is applied to the normal skin of the arm of the human body once After 20 minutes and 2 hours, the water content (AU) was measured to calculate the degree of increase (ΔAU) compared to the baseline measurement value immediately before the application of each site for each individual, and to correct the increase of the site where the control composition was applied. In the case of the limit value, the moisture content measured 20 minutes after the application of resveratrol solution, white patterned thistle extract or European horse riding extract was significantly increased compared to the control composition application site. Each composition containing galactose, fucose, and N-acetylglucosamine (N-Glc) alone did not show an increase in moisture content. In addition, at 2 hours, all the compositions did not show an increase in moisture content, but rather showed a slight decrease in some monosaccharides (see FIG. 1).

According to another embodiment of the present invention, 4-Mix 4%, GN-Mix 5%, GFN-Mix 5%, Houng Seung in order to observe the effects of various combinations of the natural extracts and monosaccharides disclosed in the present invention -Moisture content is applied 20 minutes and 2 hours after applying 6% Mix, 6% G-Mix, 11% GN-Mix, or 11% GF-Mix 11%. When the measured values were compared with the control composition-coated region in the same manner as above, after 20 minutes, the moisture content was significantly increased in all combinations except Hoong-Mix and GFN-Mix. In the case of GFN-Mix, there was no statistically significant difference between individuals, but the moisture content increased in all six patients except one, which greatly decreased, and the mean value also increased similarly to other combinations. This increase showed a similar or more increasing tendency based on the average value when compared with the application of the active ingredient alone in FIG. 1. In addition, after 2 hours, all of the compositions containing each of the active ingredients alone did not show an increase in moisture content, but when the active ingredients were combined, Hoe Seung G-Mix, Hoe Seung GN-Mix, and Hoe Seung GFN -Mix showed a significant increase in moisture even up to 2 hours after application (see Figure 2). Therefore, in the case of these three combinations, it was confirmed that the mixing of each active ingredient produces a synergistic effect, so that the moisturizing effect can be maintained for a longer time. That is, a combination of two or more components selected from the group consisting of resveratrol-containing liquid, white patterned thistle extract, European horse riding extract, glucose, galactose, fucose and N-acetylglucosamine derived from K. koji extract disclosed in the present invention, For example, 4-Mix, Glucose, GN-Mix, Houng Seung G-Mix, Houng Seung GN-Mix, or Houng Seung GFN-Mix has an effect of increasing the moisture content to 20 minutes after the minimum application, among them Houng Seung It was confirmed that G-Mix, Hoang Seung GN-Mix and Hoang Seung GFN-Mix had a significant effect of increasing the moisture content up to 2 hours after application.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are only for illustrating the present invention, and the content of the present invention is not limited by the following examples.

Example  1. Preparation of natural product extract

Ho Jang Geun, White Thistle and European Horseriding were purchased at hypermarkets and traditional markets. Ho jang-geun, white thistle and 10 g of European horseback were cut into appropriate sizes, and then extracted with purified water at 90 W for 5 minutes. The extract was filtered through Whatman filter paper and an extract of each natural product was obtained using a lyophilizer.

Experimental Example  1. Moisture increase effect of the composition containing various natural extracts from human skin as active ingredients

2% resveratrol-containing liquid derived from ephedra root extract prepared in Example 1, white thistle extract 2%, European horseback extract 2%, glucose 1%, galactose 1%, fucose 1%, After application of a composition containing 1% of N-acetylglucosamine or a control composition containing no one time, the increase or decrease of moisture content was 0 minutes (before application) and 20 minutes after application using a moisture analyzer (Corneometer ^TM ). And 2 hours after application (n = 9), respectively. The application of the composition was applied equally 15 μl in a square of 1.5 cm × 1.5 cm wide using a pipette for viscous material, and calculated the degree of increase (ΔAU) compared to the baseline measurement value immediately before the application of each part, The limit value is shown in a graph by correcting the increase amount of the control composition-coated portion.

As a result, the resveratrol-containing liquid, white-patterned thistle extract or European horse-riding extract derived from Rhodon root extract showed a significant increase in water content measured 20 minutes after application (n = 9). ), Glucose, galactose, fucose, and each composition containing N-acetylglucosamine (N-Glc) alone did not show an increase in moisture. In addition, at 2 hours after application, all the compositions did not show an increase in water content, and some monosaccharides showed a tendency to decrease slightly (FIG. 1). This suggests that when the natural extract components are applied to human skin, there is an effect of increasing the degree of moisture.

Experimental Example  2. Increasing the moisture content of the composition containing various combinations of various natural products and monosaccharides in the human skin as active ingredients

In the same manner as in Experiment 1, (1) a mixture of four monosaccharides of glucose, galactose, fucose and N-acetylglucosamine (1% each, 4% in total) (4-Mix), (2) 2% glucose , A mixture of 1% fucose, 2% N-acetylglucosamine (GFN-Mix), (3) a mixture of 2.5% glucose and 2.5% N-acetylglucosamine (GN-Mix), and (4) resveratrol from K. root extract. 2% solution, white thistle extract 2%, European horse riding extract 2% mixture (Hoong Seung-Mix), (5) resveratrol content 2%, white thistle extract 2%, European riding horse extract 2%, Glucose 5% mixture (Hungseung Seung-G-Mix), (6) Resveratrol-containing liquid derived from Rhoben root extract 2%, White thistle extract 2%, European horseriding extract 2%, Glucose 2.5%, N-acetyl A mixture of 2.5% Glucosamine (Eng-seung GN-Mix), or (7) 2% resveratrol-containing liquid derived from E. coli muscle extract, white thistle weight A composition containing a mixture of 2% water, 2% European equestrian extract, 2% glucose, 1% fucose, and 2% N-acetylglucosamine (whitfish GFN-Mix), or a control composition containing no these, was normal skin of the arm. After coating once, the moisture content was measured using a moisture meter to measure the increase and decrease of the moisture content at 0 minutes (before application), 20 minutes after application, and 2 hours after application (n = 7). The application of the composition was applied in the same 15 μl by using a pipette for viscous material in a square 1.5 cm × 1.5 cm wide, it was compared with the site where the control composition was applied.

As a result, after 20 minutes of application, the moisture content was significantly increased in all combinations except Hung-Mix and GFN-Mix (n = 7). In the case of GFN-Mix, there was a statistically insignificant difference among individuals, but it increased in all six patients except one, which greatly decreased, and the mean value also increased similarly to other combinations. This increase tended to be similar or increased on the basis of the mean value, compared with the application of the active ingredient alone in FIG. 1. This suggests that when the composition containing two or more combinations of these natural extract components and monosaccharides is applied to the skin, there is also an effect of increasing moisture.

In addition, after 2 hours of application, in the case of the compositions containing each of the active ingredients alone, no increase in moisture content was observed, but when two or more types of active ingredients were combined, Hoang Seung G-Mix and Hoang Seung GN-Mix , Or whit GFN-Mix was confirmed to show a significant increase in moisture even up to 2 hours (Fig. 2). Therefore, in the case of applying the composition containing the three combinations to the skin it was confirmed that there is an effect that the mixing of each active ingredient has a synergistic effect so that the moisturizing effect can be maintained for a longer time.

Production Example  One. Cosmetics  Preparation of the composition

1.1. Cosmetic preparation of emulsion formulations

Cosmetics of emulsion formulations were prepared with the compositions shown in Table 1. The manufacturing method is as follows.

(1) The mixture which mixed the raw materials of 1-9 was heated at 65-70 degreeC.

(2) The raw material of 10 was added to the mixture of step (1).

(3) The mixture of the raw materials of 11-13 was heated at 65-70 degreeC, and was completely dissolved.

(4) While passing through step (3), the mixture of (2) was slowly added to emulsify for 2-3 minutes at 8,000 rpm.

(5) The raw material of 14 was dissolved in a small amount of water, and then added to the mixture of step (4) and emulsified for 2 minutes.

(6) After weighing the raw materials of 15 to 19, respectively, the mixture was added to the mixture of step (5) and emulsified for 30 seconds.

(7) After the emulsification of the mixture of step (6) through a degassing process to cool to 25 ~ 35 ℃ to prepare an emulsion of cosmetics.

Furtherance Emulsifying Formulation 1 Emulsifying Formulation 2 Emulsion Formulation 3 One Stearic acid 0.3 0.3 0.3 2 Stealy alcohol 0.2 0.2 0.2 3 Glyceryl Monostearate 1.2 1.2 1.2 4 Beeswax 0.4 0.4 0.4 5 Polyoxyethylene sorbitan
Monolauric acid ester 2.2 2.2 2.2 6 Methyl paraoxybenzoate 0.1 0.1 0.1 7 Paraoxybenzoic Acid Profiles 0.05 0.05 0.05 8 Cetylethylhexanoate 5 5 5 9 Triglycerides 2 2 2 10 Cyclomethicone 3 3 3 11 Distilled water to 100 to 100 to 100 12 Dark glycerin 5 5 5 13 Triethanolamine 0.15 0.15 0.05 14 Polyacrylic acid polymer 0.12 0.12 0.12 15 Pigment 0.0001 0.0001 0.0001 16 incense 0.10 0.10 0.10 17 GFN-Mix 11 - - 18 Win-Win G-Mix - 11 - 19 4-Mix - - 4

1.2. In the proportion described in Table 2 of the solubilized cosmetic products formulated to prepare a cosmetic formulation for solubilization. The manufacturing method is as follows.

(1) The raw materials of 2-8 were put into raw material 1 (purified water), and it melt | dissolved using the still mixer.

(2) 10 to 13 raw materials were placed in 9 raw materials (alcohol) and completely dissolved.

(3) The mixture of step 2) was solubilized with slow addition to the mixture of step 1).

Furtherance Solubilized Formulation 1 Solubilized Formulation 2 Solubilized Formulation 3 One Purified water to 100 to 100 to 100 2 Dark glycerin 3 3 3 3 1,3-butylene glycol 2 2 2 4 EDTA-2Na 0.01 0.01 0.01 5 Pigment 0.0001 0.0002 0.0002 6 4-Mix 4 - - 7 Win-Win GN-Mix - 11 - 8 GN-Mix - - 5 9 Alcohol (95%) 8 8 8 10 Methyl paraoxybenzoate 0.1 0.1 0.1 11 Polyoxyethylene
Hydrogenate diester 0.3 0.3 0.3 12 incense 0.15 0.15 0.15 13 Cyclomethicone - - 0.2

Production Example  2. of pharmaceutical composition Manufacture 2 .One. Preparation of skin external ointments

Skin external ointment containing the composition of the present invention as an active ingredient was prepared as shown in Table 3.

Raw material Content (parts by weight) Purified water 100 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 GFN-Mix 11.0 Caprylic Capric Triglycerides 3.0 Squalane 1.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Cetearyl Alcohol 1.0 antiseptic Quantity incense Quantity Pigment Quantity Beeswax 4.0

2.2. Of skin external patch Preparation A skin external patch containing the composition of the present invention as an active ingredient was prepared as shown in Table 4 below.

Raw material Content (parts by weight) Win-Win G-Mix 11.0 Hexylene glycol 20.0 Diethylamine 0.7 Polyacrylic acid 1.0 Sodium sulfite 0.1 Polyoxyethylene lauryl ether (E.0 = 9) 1.0 Polyhydroxyethylene cetyl stearyl ether 1.0 Viscous Paraffin Oil 2.5 Caprylic Acid Ester / Capric Acid Ester 2.5 Polyethylene Glycol 400 3.0 Deionized water 70

Claims (14)

It contains as an active ingredient two or more selected from the group consisting of extracts of kojang-geun, white-patterned thistle, European horseback riding extract, glucose, galactose, fucose and N-acetylglucosamine,
The active ingredient is
(1) a mixture of glucose, galactose, fucose and N-acetylglucosamine;
(2) a mixture of glucose, fucose and N-acetylglucosamine;
(3) a mixture of glucose and N-acetylglucosamine;
(4) a mixture of rhododendron extract, white thistle extract and european horseback extract;
(5) a mixture of rhododendron extract, white thistle extract, european horse riding extract and glucose;
(6) a mixture of rhododendron extract, white thistle extract, european horse riding extract, glucose and N-acetylglucosamine; And
(7) a mixture of rhododendron extract, white thistle extract, european horse riding extract, glucose, fucose and N-acetylglucosamine;
Selected from the group consisting of
The extract Kwon Keun Root cosmetic composition for increasing skin moisture containing resveratrol. delete The method according to claim 1,
(1) The mixture of glucose, galactose, fucose and N-acetylglucosamine is mixed in a weight ratio of glucose: galactose: fucose: N-acetylglucosamine = 1: 0.5-2: 0.5-2: 0.5-2,
(2) the mixture of glucose, fucose and N-acetylglucosamine is mixed in a weight ratio of glucose: fucose: N-acetylglucosamine = 2: 0.5 to 2: 1 to 4,
(3) the mixture of glucose and N-acetylglucosamine is mixed in a weight ratio of glucose: N-acetylglucosamine = 1: 0.5 to 2,
(4) the mixture of the rye root extract, white patterned thistle extract and the European riding horse extract is mixed in a weight ratio of rye root extract: white patterned thistle extract: European riding horse extract = 1: 0.5 to 2: 0.5 to 2,
(5) The mixture of the rye root extract, white thistle extract, the European horse riding extract and the glucose may include the rye root extract: the white horse thistle extract: the European horse riding extract: glucose = 2: 1 to 4: 1 to 4: 2: 8 Mixed in weight ratio,
(6) The mixture of the rhododendron extract, white thistle extract, the European riding horse extract, glucose, and N-acetylglucosamine may include the rhododendron extract: the white rye thistle extract: the European riding horse extract: glucose: N-acetylglucosamine = 2: 1 to 4: 1 to 4: 1 to 5: 1 to 5 in a weight ratio of mixed, and
(7) The mixture of the rhododendron extract, white thistle extract, the European riding horse extract, glucose, fucose and N-acetylglucosamine may include the rhododendron extract: the white rye thistle extract: the European riding horse extract: glucose: fucose: N-acetyl Glucosamine = 2: 1-4: 1-4: 1-4: 0.5-2: 1-4 The cosmetic composition mixed with the weight ratio. delete The method according to claim 1,
The active ingredient is contained in an amount of 0.001 to 10% by weight of the total composition. The method according to claim 1,
Cosmetic composition having a skin external preparation formulation. The method according to claim 6,
The formulation is a cosmetic composition selected from the group consisting of softening cosmetics, nourishing cosmetics, massage cream, nutrition cream, pack, gel, lotion, gel, cream, patch and spray. It contains as an active ingredient two or more selected from the group consisting of extracts of kojang-geun, white-patterned thistle, European horseback riding extract, glucose, galactose, fucose and N-acetylglucosamine,
The active ingredient is
(1) a mixture of glucose, galactose, fucose and N-acetylglucosamine;
(2) a mixture of glucose, fucose and N-acetylglucosamine;
(3) a mixture of glucose and N-acetylglucosamine;
(4) a mixture of rhododendron extract, white thistle extract and european horseback extract;
(5) a mixture of rhododendron extract, white thistle extract, european horse riding extract and glucose;
(6) a mixture of rhododendron extract, white thistle extract, european horse riding extract, glucose and N-acetylglucosamine; And
(7) a mixture of rhododendron extract, white thistle extract, european horse riding extract, glucose, fucose and N-acetylglucosamine;
Selected from the group consisting of
The extract Kwon Keun-Geun is a pharmaceutical composition for increasing skin moisture of a disease selected from the group consisting of dry skin, eczema, psoriasis, atopic dermatitis, senile gland and pemphigus containing resveratrol. delete The method according to claim 8,
(1) The mixture of glucose, galactose, fucose and N-acetylglucosamine is mixed in a weight ratio of glucose: galactose: fucose: N-acetylglucosamine = 1: 0.5-2: 0.5-2: 0.5-2,
(2) the mixture of glucose, fucose and N-acetylglucosamine is mixed in a weight ratio of glucose: fucose: N-acetylglucosamine = 2: 0.5 to 2: 1 to 4,
(3) the mixture of glucose and N-acetylglucosamine is mixed in a weight ratio of glucose: N-acetylglucosamine = 1: 0.5 to 2,
(4) the mixture of the rhododendron extract, white pattern thistle extract, and the European horse riding extract is mixed in a weight ratio of rhododendron extract: white pattern thistle extract: the European riding horse extract = 1: 0.5-2: 0.5-2;
(5) The mixture of the rye root extract, white thistle extract, the European horse riding extract and the glucose may include the rye root extract: the white horse thistle extract: the European horse riding extract: glucose = 2: 1 to 4: 1 to 4: 2: 8 Mixed in weight ratio,
(6) The mixture of the rhododendron extract, white thistle extract, the European riding horse extract, glucose, and N-acetylglucosamine may include the rhododendron extract: the white rye thistle extract: the European riding horse extract: glucose: N-acetylglucosamine = 2: 1 to 4: 1 to 4: 1 to 5: 1 to 5 in a weight ratio of mixed, and
(7) The mixture of the rhododendron extract, white thistle extract, the European riding horse extract, glucose, fucose and N-acetylglucosamine may include the rhododendron extract: the white rye thistle extract: the European riding horse extract: glucose: fucose: N-acetyl Glucosamine = 2: 1 to 4: 1 to 4: 1 to 4: 0.5 to 2: 1 to 4. delete The method according to claim 8,
The active ingredient is a pharmaceutical composition containing 0.001 to 10% by weight of the total composition. The method according to claim 8,
Wherein said pharmaceutical composition is in the form of a parenteral dosage form selected from the group consisting of solid, semisolid and liquid. It contains as an active ingredient two or more selected from the group consisting of extracts of kojang-geun, white-patterned thistle, European horseback riding extract, glucose, galactose, fucose and N-acetylglucosamine,
The active ingredient is
(1) a mixture of glucose, galactose, fucose and N-acetylglucosamine;
(2) a mixture of glucose, fucose and N-acetylglucosamine;
(3) a mixture of glucose and N-acetylglucosamine;
(4) a mixture of rhododendron extract, white thistle extract and european horseback extract;
(5) a mixture of rhododendron extract, white thistle extract, european horse riding extract and glucose;
(6) a mixture of rhododendron extract, white thistle extract, european horse riding extract, glucose and N-acetylglucosamine; And
(7) a mixture of rhododendron extract, white thistle extract, european horse riding extract, glucose, fucose and N-acetylglucosamine;
Selected from the group consisting of
The extract Kwon Root is a health food for increasing skin moisture containing resveratrol.

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