KR101555658B1 - 재조합 폴리클로날 단백질의 제조 방법 - Google Patents

재조합 폴리클로날 단백질의 제조 방법 Download PDF

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KR101555658B1
KR101555658B1 KR1020097027038A KR20097027038A KR101555658B1 KR 101555658 B1 KR101555658 B1 KR 101555658B1 KR 1020097027038 A KR1020097027038 A KR 1020097027038A KR 20097027038 A KR20097027038 A KR 20097027038A KR 101555658 B1 KR101555658 B1 KR 101555658B1
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polyclonal
cells
cell
delete delete
protein
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KR20100017923A (ko
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라르스 소에가아르트 니엘센
디트마르 웨일쿠니
안네 본트가아르트 톨스트럽
핀 비베르그
크리스티안 뮐러
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심포젠 에이/에스
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081—DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Non-Volatile Memory (AREA)
KR1020097027038A 2007-05-25 2008-05-21 재조합 폴리클로날 단백질의 제조 방법 Expired - Fee Related KR101555658B1 (ko)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
DKPA200700764 2007-05-25
DKPA200700764 2007-05-25
US92470807P 2007-05-29 2007-05-29
US60/924,708 2007-05-29
DKPA200701292 2007-09-07
DKPA200701292 2007-09-07
US96000207P 2007-09-11 2007-09-11
US60/960,002 2007-09-11

Publications (2)

Publication Number Publication Date
KR20100017923A KR20100017923A (ko) 2010-02-16
KR101555658B1 true KR101555658B1 (ko) 2015-09-24

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KR1020097027038A Expired - Fee Related KR101555658B1 (ko) 2007-05-25 2008-05-21 재조합 폴리클로날 단백질의 제조 방법

Country Status (22)

Country Link
US (1) US7910332B2 (enExample)
EP (1) EP2152872B1 (enExample)
JP (2) JP5941616B2 (enExample)
KR (1) KR101555658B1 (enExample)
CN (1) CN101688204B (enExample)
AT (1) ATE481483T1 (enExample)
AU (1) AU2008255383B2 (enExample)
BR (1) BRPI0812091A2 (enExample)
CA (1) CA2683800C (enExample)
CY (1) CY1110939T1 (enExample)
DE (1) DE602008002593D1 (enExample)
DK (1) DK2152872T3 (enExample)
HR (1) HRP20100640T1 (enExample)
IL (1) IL201395A (enExample)
MX (1) MX2009012526A (enExample)
NZ (1) NZ581316A (enExample)
PL (1) PL2152872T3 (enExample)
PT (1) PT2152872E (enExample)
SI (1) SI2152872T1 (enExample)
TW (1) TW200902548A (enExample)
WO (1) WO2008145133A2 (enExample)
ZA (1) ZA200908021B (enExample)

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EA013225B1 (ru) 2003-01-07 2010-04-30 Симфоген А/С Способ получения линии клеток, продуцирующей рекомбинантный поликлональный белок, способ получения поликлонального белка, линия клеток, продуцирующая рекомбинантный поликлональный белок, библиотека векторов, популяция клеток
NZ578851A (en) * 2007-03-01 2011-09-30 Symphogen As Method for cloning cognate antibodies
BRPI0812091A2 (pt) * 2007-05-25 2014-10-14 Symphogen As Método para fabricar uma proteína policlonal recombinante
AU2008295248A1 (en) * 2007-09-07 2009-03-12 Symphogen A/S Methods for recombinant manufacturing of anti-RSV antibodies
NZ601353A (en) * 2008-02-01 2014-06-27 Chromocell Corp Cell lines and methods for making and using them
EP2098536A1 (en) 2008-03-05 2009-09-09 4-Antibody AG Isolation and identification of antigen- or ligand-specific binding proteins
RU2010147652A (ru) * 2008-04-23 2012-05-27 Симфоген А/С (Dk) Способы производства поликлонального белка
EP2297331B1 (en) * 2008-05-28 2015-03-25 Bayer HealthCare, LLC Methods and compositions for production of recombinant protein in hbx-expressing mammalian cells
CA2735020A1 (en) * 2008-08-29 2010-03-04 Symphogen A/S Method for cloning avian-derived antibodies
WO2010089387A1 (en) 2009-02-09 2010-08-12 Morphosys Ag Production of oligoclonal mixtures of immunoglobulins in single cells
WO2012059857A2 (en) 2010-11-01 2012-05-10 Symphogen A/S Pan-her antibody composition
JP6092782B2 (ja) 2010-11-16 2017-03-08 エクセリミューン， インコーポレイテッド 組換えタンパク質の製造方法
CN102558353A (zh) * 2011-12-09 2012-07-11 上海应用技术学院 一种大肠杆菌NrfA基因表达蛋白多克隆抗体及其制备方法
GB2502127A (en) 2012-05-17 2013-11-20 Kymab Ltd Multivalent antibodies and in vivo methods for their production
HK1202431A1 (en) 2012-05-02 2015-10-02 Symphogen A/S Humanized pan-her antibody compositions
JP2016514091A (ja) 2013-02-08 2016-05-19 ミスフォールディング ダイアグノスティクス， インコーポレイテッド トランスサイレチン抗体およびその使用
MX378108B (es) 2014-09-16 2025-03-10 Servier Lab Anticuerpos anti-met y composiciones.
US12404552B2 (en) * 2015-07-17 2025-09-02 Allele Biotechnology & Pharmaceuticals, Inc. Methods of selecting antibodies and antibody fragments
WO2017121896A1 (en) * 2016-01-14 2017-07-20 Anton Bauer Method of producing a production cell line
WO2018073393A2 (en) 2016-10-19 2018-04-26 Cellectis Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy
AU2018247916B2 (en) 2017-04-05 2025-04-24 Les Laboratoires Servier Combination therapies targeting PD-1, TIM-3, and LAG-3
US12247065B2 (en) 2018-06-22 2025-03-11 Genmab A/S Method for producing a controlled mixture of two or more different antibodies
KR102559149B1 (ko) 2018-10-26 2023-07-24 에프. 호프만-라 로슈 아게 재조합효소 매개 카세트 교환을 이용한 다중특이적 항체 스크리닝 방법
CA3118692A1 (en) 2018-11-06 2020-05-14 Alsatech, Inc. Cell-based gene therapy for neurodegenerative diseases
CN109988751A (zh) * 2019-01-15 2019-07-09 济宁学院 一种用于制备抗egfr全人源化单克隆抗体的细胞株构建方法
JP2022531113A (ja) 2019-04-30 2022-07-06 ギガジェン，インコーポレイティッド 組換えポリクローナルタンパク質およびその使用方法
EP4034637A1 (en) * 2019-09-27 2022-08-03 GlaxoSmithKline Intellectual Property Development Limited Method of predicting production stability of clonal cell lines
WO2021253002A1 (en) 2020-06-12 2021-12-16 Gigagen, Inc. Recombinant polyclonal proteins targeting covid-19 and methods of use thereof
AU2024277829A1 (en) 2023-05-19 2025-12-04 Les Laboratoires Servier Anti-met antibodies, antibody-drug conjugates, compositions and uses thereof

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WO2004029284A3 (en) 2002-09-30 2005-05-12 Protein Design Labs Inc Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes

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US6111166A (en) 1994-09-19 2000-08-29 Medarex, Incorporated Transgenic mice expressing human Fcα and β receptors
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US6849259B2 (en) * 2000-06-16 2005-02-01 Symphogen A/S Polyclonal antibody composition for treating allergy
NZ537277A (en) * 2002-07-18 2008-04-30 Crucell Holland Bv Recombinant production of mixtures of antibodies
CN100383244C (zh) * 2003-01-07 2008-04-23 西福根有限公司 用于生产重组多克隆蛋白质的方法
US20060275766A1 (en) * 2003-01-07 2006-12-07 Haurum John S Method for manufacturing recombinant polyclonal proteins
EA013225B1 (ru) * 2003-01-07 2010-04-30 Симфоген А/С Способ получения линии клеток, продуцирующей рекомбинантный поликлональный белок, способ получения поликлонального белка, линия клеток, продуцирующая рекомбинантный поликлональный белок, библиотека векторов, популяция клеток
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Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
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Publication number Publication date
DE602008002593D1 (de) 2010-10-28
CA2683800C (en) 2015-03-17
HK1134519A1 (en) 2010-04-30
JP2015037411A (ja) 2015-02-26
PL2152872T3 (pl) 2011-03-31
AU2008255383A1 (en) 2008-12-04
BRPI0812091A2 (pt) 2014-10-14
ZA200908021B (en) 2010-11-24
EP2152872A2 (en) 2010-02-17
CA2683800A1 (en) 2008-12-04
WO2008145133A3 (en) 2009-02-05
DK2152872T3 (da) 2010-12-06
IL201395A (en) 2015-09-24
AU2008255383B2 (en) 2012-11-01
WO2008145133A2 (en) 2008-12-04
CN101688204A (zh) 2010-03-31
PT2152872E (pt) 2010-11-17
JP2011504721A (ja) 2011-02-17
NZ581316A (en) 2012-02-24
MX2009012526A (es) 2009-12-09
EP2152872B1 (en) 2010-09-15
CY1110939T1 (el) 2015-06-10
US7910332B2 (en) 2011-03-22
CN101688204B (zh) 2013-07-03
KR20100017923A (ko) 2010-02-16
JP5941616B2 (ja) 2016-06-29
ATE481483T1 (de) 2010-10-15
HRP20100640T1 (hr) 2010-12-31
US20090111142A1 (en) 2009-04-30
SI2152872T1 (sl) 2011-01-31
IL201395A0 (en) 2011-08-01
TW200902548A (en) 2009-01-16

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