KR101550489B1 - 기준 차단 서열을 이용한 완전 cold-pcr 풍부화 - Google Patents

기준 차단 서열을 이용한 완전 cold-pcr 풍부화 Download PDF

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KR101550489B1
KR101550489B1 KR1020127026077A KR20127026077A KR101550489B1 KR 101550489 B1 KR101550489 B1 KR 101550489B1 KR 1020127026077 A KR1020127026077 A KR 1020127026077A KR 20127026077 A KR20127026077 A KR 20127026077A KR 101550489 B1 KR101550489 B1 KR 101550489B1
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sequence
pcr
reaction mixture
nucleic acid
strand
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KR20120139780A (ko
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제라시모스 마크리지오고스
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다나-파버 캔서 인스티튜트 인크.
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/107Temperature of melting, i.e. Tm

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KR1020127026077A 2010-03-08 2011-03-08 기준 차단 서열을 이용한 완전 cold-pcr 풍부화 Expired - Fee Related KR101550489B1 (ko)

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US31164210P 2010-03-08 2010-03-08
US61/311,642 2010-03-08
PCT/US2011/027473 WO2011112534A1 (en) 2010-03-08 2011-03-08 Full cold-pcr enrichment with reference blocking sequence

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KR1020157014456A Withdrawn KR20150067398A (ko) 2010-03-08 2011-03-08 기준 차단 서열을 이용한 완전 cold-pcr 풍부화

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US (3) US8623603B2 (https=)
EP (1) EP2545189B1 (https=)
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CN (1) CN102859003B (https=)
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