KR101480693B1 - Anti-aging cosmetic composition for skin - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging which restores skin density and gloss to make the skin younger. More specifically, it contains a mixture of apple stem cells, hibiscus seed extract and argan oil to solve the fine lines, dullness, coarse skin texture and protect growth factors that decrease with age in the skin. And to provide a cosmetic composition for preventing skin aging which exhibits an excellent effect in restoring skin luster.

Stem Cells * Hibiscus Seed Extract * Argan Oil * Improved Wrinkles * Increased Skin Density * Skin Gloss Recovery * Anti-Aging

Description

[0001] Anti-aging cosmetic composition for skin [0002]

The invention apple stem cells, hibiscus (Hibiscus esculentus seed extract and argan oil to resolve fine wrinkles, dullness, rough skin texture, and protects growth factors that decrease with age in the skin, promoting collagen synthesis to increase skin density And exhibits an excellent effect on restoration of skin glossiness.

Skin is the primary barrier of the human body and protects the internal organs of the body from changes in temperature and humidity, from external stimuli such as ultraviolet rays and pollutants, and plays an important role in the maintenance of body homeostasis such as body temperature control. As with other organs in the human body, such skin gradually ages as a person ages, resulting in skin elasticity loss, keratinization, wrinkle formation, spots, freckles and blurredness. The cause of skin aging can be classified into internal factors caused by gene mutations in cells, and extrinsic factors such as ultraviolet rays and humidity (Masamitsu Ichihashi, Fragrance Journal, Vol. 32, No. 5, 24-30).

First of all, structural changes of the skin due to aging will reduce the thickness of the epidermis, dermis and subcutaneous tissue components of the skin. In addition, the extracellular matrix (ECM) component of the dermal tissue responsible for skin elasticity and tensile changes. Collagen accounts for about 70-80% of the total ECM. As aging progresses, the elasticity of the skin is greatly reduced, which is due to the reduction of collagen and elastin. Various materials have been developed for the purpose of inhibiting collagen and elastin reduction. In particular, retinoids such as retinol and retinoic acid have been known to improve wrinkles or improve elasticity (Dermatology therapy, 1998, 16, 357-364). However, these retinoids suffer from the disadvantage that irritation occurs even when only a small amount of the retinoid is applied to the skin, and they are easily oxidized by exposure to air due to instability. Accordingly, there has been a need to develop active ingredients capable of achieving skin aging prevention effects without causing skin side effects.

The injury wounded in childhood can easily heal without scarring, but the wound after the adult becomes slow and scar remains. These changes result from the difference in the natural healing power of the skin and the regenerative power of the cells. The natural healing power of the skin and the regeneration power of the cells are inherent characteristics of the stem cells existing in the epidermal basal layer of the skin. Stem cells play a key role in completely regulating the regeneration and healing process by repeatedly differentiating in each region at a certain period while humans are alive, and play an important role in maintaining the homeostasis of the living body.

It is known that about 2 ~ 7% of stem cells are present in the epidermal basal layer of the skin. It is very sensitive due to the internal and external harmful environment. It gradually declines due to aging and loses its stemness, It gradually decreases. If the skin cells lose their ability or lose their ability, the skin cells to be born and replaced in a timely manner are not made well, and the skin's underlying regenerative power is lost, so various skin symptoms such as fine lines, dullness, do. Therefore, it is important to protect and maintain the natural stem cells from these internal and external factors in order to maintain healthy and beautiful skin.

The present inventors have found that the present invention can be used as an effective ingredient for maintaining the skin younger by promoting the synthesis of collagen and the like by protecting the skin stem cells and prolonging the life of skin cells, protecting skin cells from various UV or stress, As a result of efforts to find out, it has been found that a mixture of plant stem cells, hibiscus seed extract and argan oil can obtain a good anti-aging effect on the skin, thus completing the present invention.

Accordingly, it is an object of the present invention to provide a cosmetic composition for preventing skin aging which contains plant stem cells, hibiscus seed extract and argan oil as effective ingredients, which have excellent efficacy for inhibiting skin aging.

In order to achieve the above object, the present invention provides a cosmetic composition for preventing skin aging comprising apple stem cells, hibiscus seed extract and argan oil as an active ingredient.

In addition, in the present invention, at least one selected from the group consisting of oat seed extract, hyaluronic acid, chitosan, soybean extract, polyglyceryl-8 stearate, ceramide, phytosphingosine and yeast extract is further added And a cosmetic composition for preventing aging of the skin.

The anti-aging cosmetic composition according to the present invention contains plant stem cells, Hibiscus seed extract and argan oil as an active ingredient to prolong skin cell life, restore skin density and gloss, , Skin dullness and shrinkage are improved to exhibit an excellent anti-aging effect.

The present invention provides a cosmetic composition for preventing skin aging comprising a mixture of apple stem cells, hibiscus seed extract and argan oil.

The anti-aging cosmetic composition according to the present invention preferably contains 1.0 to 3.0% by weight of apple stem cells, 0.5 to 1.0% by weight of hibiscus seed extract, and 0.5 to 1.0% by weight of argan oil, based on the total weight of the composition Do.

Hereinafter, the present invention will be described in more detail.

As the skin gets older, the density of stem cells decreases, causing the skin regenerative power to fall, cause rough skin, and reduce skin tone. Aging skin can be improved through stem cell protection and activation.

When the plant is attacked or injured from outside, the cells around the damaged tissue return to the regenerative baby cells (plant stem cells), resulting in an intensive and rapid wound regeneration process. When the wound healing ends, It returns to the original normal cells. Plant stem cells are rich in plant stem cell factors and various metabolites, which not only improve the performance of skin stem cells but also activate the anti-aging protein, p63, to prolong the life of skin cells.

Researchers say p63, a cancer-suppressing gene, is associated with aging. The absence of the p63 gene has been shown to promote senescence, and studies have shown that the p63 gene has been removed from the multiparous rats, resulting in premature aging and shortened lifespan. Thus, p63 is likely to be a gene that performs a basic biological role in the aging process. All of the rodents born with either no p63 gene or only one of the two pairs died prematurely, and after removing both pairs of p63 genes in specific tissues, such as in the skin of the multiparous rats, there was a tendency for hair loss, weight loss and progressive curvature of the vertebrae And the life span is shortened. Aging and cancer are both sides of a coin. Cells either stop or do not stop dividing. The p63 gene's proteins must be kept in proper cells at the right time, so that the tissue can survive for a long time without cancer. .

The method of obtaining apple stem cells of the present invention is not so complicated. When you sharpen the outer surface of an apple, it will regenerate because of the unique response of the plant. At this time, the regeneration site is transferred to a culture plate, and cells are proliferated through a general cell culture method. Then, the cells grow on the medium, and a large amount of apple stem cells is obtained. The reason for choosing apples among many plants is that the proliferation of stem cells of apple is faster than other plants and it is suitable for use as a raw material for cosmetics because a large amount of stem cells can be obtained.

The cosmetic composition for anti-aging according to the present invention preferably contains sieve and stem cells in an amount of 1.0 wt% to 3.0 wt% based on the total weight of the composition. When the content is less than 1.0% by weight, the effect of the apple stem cell is insignificant. When the content is more than 3.0% by weight, the effect is equivalent to the effect of 3.0% by weight or less.

Hibiscus The line factor, a seed extract, is a plant native to the equator and widely known for its relaxation and stimulation effects. Hibiscus seed extract has a raw material name called a line factor, which is a unique name given by the raw material manufacturer. Hibiscus seed extract protects FGF-2 from fibroblasts and stimulates collagen GAG synthesis to form young skin. When FGF-2 is produced in fibroblasts, it binds to proteoglycan. FGF-2, which is stabilized by binding with proteoglycan, binds to FGFR again to regenerate the skin. Cell multiplication, collagen, And GAG synthesis.

Hibiscus seed extract is known to promote collagen synthesis by concentration. It is known that 1% of collagen synthesis promotes collagen synthesis by 18% and 3% of collagen synthesis promotes collagen synthesis by 23%. When 2% of GAG synthesis is also contained, it promotes synthesis of about 294%. It is known that when 1% is used, GAG synthesis effect is 1.8 times and 1.3 times that of vitamin C and pentapeptide, respectively. Clinical trials have shown that skin tissue is improved by 50% to 100% over 3% of vitamin C when using 3% of hibiscus seed extract. Skin elasticity was improved by about 37% in the 50-60 age group and wrinkle improvement was about 2.2 times more effective than vitamin C in the Hibiscus seed extract. After 42 days, skin density in the 50-60 age group was the same as that in the 40-50 age group, and skin tissue was improved in the 50-60 age group than in the 40-50 age group.

The hibiscus seed extract is extracted by a known method. The seeds of the hibiscus are dried and pulverized and extracted with water or an organic solvent. Examples of the organic solvent include polar solvents such as ethanol, butanol, and acetone, and nonpolar solvents such as ether, hexane, benzene, chloroform, and ethyl acetate, or mixtures thereof.

The anti-aging cosmetic composition according to the present invention preferably contains a hibiscus seed extract in an amount of 0.5 to 1.0 wt% based on the total weight of the composition. If the content is less than 0.5% by weight, the effect of the hibiscus seed extract is very small. If the content is more than 1.0% by weight, the effect is equivalent to 1.0% by weight or less.

Argan oil is highly concentrated in tocopherol and forms a protective barrier for skin cells, restoring the softness of the skin. It also instantly restores the damaged skin from the external environment and protects it completely from external environment. Argan oil also has the effect of restoring health as a food. Argan oil is rich in unsaturated fatty acids, so it is effective in healing heart disease, has the effect of producing male sperm, and the ingredient is rich in phytosterol which helps brain activity.

In addition to its efficacy in health, it has excellent efficacy in the skin. It is known as a miracle-like extract that keeps youthful. It not only regenerates skin but strengthens hair and nails. Skin moisturizing effect is equivalent to 10 times of olive oil. Vitamin E is twice the antioxidant effect of olive oil This is very good. Moroccan women are known to have used argan oil to protect their skin in the harsh climate of the desert for centuries. Argan oil produced in Morocco is all handmade by traditional methods and is also known to be very environmentally friendly.

A common recipe for argan oil is argania spinosa ) is milled on a millstone and kneaded by hand until oil seeps out, then the oil is collected to extract pure oil. In order to extract 1 liter of oil, on average, a single tree of fruit is needed and the amount is about 125 kilograms.

The anti-aging cosmetic composition according to the present invention preferably contains argan oil in an amount of 0.5 to 1.0% by weight based on the total weight of the composition. If the amount is less than 0.5% by weight, the effect of the argan oil becomes very low. If the amount of the argan oil is more than 1.0% by weight, the amount of the oil increases and the product itself becomes too oily in terms of feeling.

The cosmetic composition for preventing aging according to the present invention may be selected from the group consisting of oat seed extract, hyaluronic acid, chitosan, soybean extract, polyglyceryl-8 stearate, ceramide, phytosphingosine and yeast extract together with the above three components Based on the total weight of the cosmetic composition, in an amount of 0.5% by weight to 5.0% by weight. If the amount is less than 0.5% by weight, the effect of each component becomes very insignificant. If the amount of the component exceeds 5.0% by weight, the efficacy of the composition is deteriorated due to the cross-reaction of different components.

The oat seed extract of the present invention imparts elasticity to the skin, and hyaluronic acid, chitosan, soybean extract and yeast extract have an effect of moisturizing and supplying nutrients to the skin. Polyglyceryl-8 stearate, ceramide and phytosphingosine provide vitality and energy to the skin.

The cosmetic composition for preventing aging according to the present invention is not particularly limited in its formulation. For example, softening longevity, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil, body An essence, a makeup base, or a foundation, and may be formulated into a cosmetic for preventing skin aging or a medicament such as ointment and patch.

Hereinafter, the present invention will be described in more detail by way of examples and comparative examples, but the present invention is not limited to these examples.

[Example 1 and Comparative Example 1]

Example 1 and Comparative Example 1 were prepared in cosmetic formulations in the composition shown in Table 1 below (unit: wt%). The hibiscus seed extract used in the present invention was purchased from BASF, the stem cell was purchased from Mibelle, and argan oil was purchased from Morocco.

ingredient Comparative Example 1
(Unit: wt%) Example 1
(Unit: wt%) Distilled water To 100 To 100 glycerin 1.0 1.0 Butylene glycol 2.0 2.0 Dipropylene glycol 3.0 3.0 Hibiscus seed extract - 1.0 Apple stem cell - 3.0 Argan oil - 1.0 Polyglyceryl-3 methyl glucoside distearate 0.5 0.5 Cetyl 2-ethylhexanoate 4.0 4.0 Meadow Foam Seed Oil 3.0 3.0 Cetostearyl alcohol 3.0 3.0 Methyl paraben 0.2 0.2 Propylparaben 0.1 0.1 Hydroxyethyl acrylate. Sodium acryloyldimethyltaurate copolymer 0.8 0.8

Thirty women from 30 to 50 Korean women were divided into the above two groups for 6 weeks, and the cosmetic composition prepared in the Examples and Comparative Examples was used twice a day, Respectively.

Evaluation items were measured by using a corneometer, dermatological status and mass measurement using D-squame black tape, skin roughness and wrinkle measurement using PRISMO, skin density measurement using Dermascan C, skin gloss measurement using Sebumeter, Skin brightness and subjective evaluation by questionnaire.

[Test Example 1] Measurement of moisture resistance

Moisture power, that is, moisture change was measured at a point 2 weeks, 4 weeks, and 6 weeks after application of the composition of the example according to the present invention and using a corneometer, and the results are shown in FIG. 1 .

As can be seen in FIG. 1, after about 6 weeks of application of the composition of the examples according to the present invention, the skin moisture content was improved by about 31%, and the dry, dry skin was restored to moist and smooth skin. On the other hand, in the case of the comparative example, the moisture resistance was reduced to 30% or less as compared with the example.

[Test Example 2] Measurement of keratin condition and mass

The skin keratin state and each mass were measured before application of the composition of the example according to the present invention and after 2 weeks, 4 weeks, and 6 weeks after application using D-squame black tape. The results are shown in FIG. 2 .

As can be seen in FIG. 2, after about 6 weeks of application of the composition of the example according to the present invention, the amount of keratinous matter decreased by about 21%.

FIG. 3 is a microscopic image showing the state of the skin keratin. It can be confirmed that the skin keratinous state is improved and the skin is smooth and soft. On the other hand, in the case of the comparative example, the keratin remains unchanged and the amount of keratin is considerably large, thereby showing a rough skin surface.

[Test Example 3] Measurement of skin glossiness

Skin gloss was measured at 2 weeks, 4 weeks, and 6 weeks after application of the compositions of the examples according to the present invention and using Seubumeter. The results are shown in FIG.

As can be seen in FIG. 4, skin gloss was increased by about 32% after 6 weeks of application of the composition of the example according to the present invention.

The shine-improving effect of the composition is shown in FIG. The skin luster at the age of 47 years (mean age) at the time when the composition according to the present invention was not applied was only about 78% of the skin luster at the age of 35 (average age) To 12 years younger than the original age. On the other hand, in the case of the comparative example, the skin glossiness of about 8% was increased after 6 weeks of application of the composition of the comparative example.

[Test Example 4] Skin roughness and wrinkle measurement

Skin roughness was measured before application of the composition of the example according to the present invention and at 2 weeks, 4 weeks, and 6 weeks after application using PRISMO. The results are shown in FIGS. 6 and 7.

As can be seen from Figs. 6 and 7, the compositions of the examples of the present invention showed significant improvement in skin roughness and wrinkles after 6 weeks of application.

FIG. 8 is a three-dimensional image of skin roughness and wrinkle state measured using PRISMO. It can be seen that after 6 weeks of use of the composition according to the present invention, the wrinkles became thinner and the skin roughness was much improved. On the other hand, in the case of the comparative example, the composition of the comparative example was applied, and there was no significant difference between after 6 weeks and before application.

[Test Example 5] Measurement of skin density

Skin density was measured before application of the composition of the example according to the present invention and at 2 weeks, 4 weeks, and 6 weeks after application using Dermascan C, and the results are shown in FIG.

As can be seen from FIG. 9, the composition of the example according to the present invention showed a marked increase in skin density after 6 weeks of use because the skin density gradually increased.

FIG. 10 shows the results of skin density measurement by age group. The skin density of 47 years old (average age) after 4 weeks of use of the composition of the present invention was 35 years old (average age) It can be seen that the skin density is improved.

Figure 11 is a Dermascan image showing the change in skin density, showing that the skin density gradually increases with the duration of use of the composition of the present invention. On the other hand, in the case of the comparative example, there was no difference in skin density between 6 weeks after application of the composition and before application.

[Test Example 6] Measurement of skin brightness

The improvement in skin brightness was measured at 2 weeks, 4 weeks, and 6 weeks after application of the compositions of the examples according to the present invention and using the Chromameter. The results are shown in FIG.

As can be seen from FIG. 12, the compositions of the examples of the present invention showed excellent skin brightness improvement after 6 weeks of use.

FIG. 13 shows the results of measuring the skin brightness according to ages. When the composition of the present invention was used, the skin brightness at 47 years (average age) was improved to 35 years (average age) after 6 weeks. On the other hand, in the case of the comparative example, there was no effect in improving the skin brightness when the composition was applied 6 weeks later and before application.

[Test Example 7] Evaluation of subjective efficacy

At the time of completion of the above Test Examples 1 to 6, subjects were asked to fill out a questionnaire on the compositions of the examples and the comparative examples, and the subjective efficacy was evaluated. The satisfaction results are shown in Table 2 below.

Skin improvement effect Feeling Comprehensive satisfaction lubricity Skin tone improvement Increased skin gloss Absorption degree Brittle Make-up well Example 90% 63% 77% 88% 70% 93% 90% Comparative Example 60% 21% 33% 80% 65% 70% 55%

FIG. 1 is a graph showing the results of skin moisture content measurement after application of the cosmetic composition according to the present invention. FIG.

Fig. 2 is a graph showing the results of measuring the respective masses after application of the cosmetic composition according to the present invention. Fig.

FIG. 3 is an image of a keratinocyte using D-squame black tape showing skin keratin state before and after application of the cosmetic composition according to the present invention.

4 is a graph showing the results of measurement of skin gloss after application of the cosmetic composition according to the present invention.

FIG. 5 is a graph showing the results of analysis of age-related improvement of skin glossiness after application of the cosmetic composition according to the present invention.

6 is a graph showing the results of measurement of skin roughness after application of the cosmetic composition according to the present invention.

FIG. 7 is a graph showing the results of measurement of skin wrinkles after application of the cosmetic composition according to the present invention. FIG.

FIG. 8 is a three-dimensional image of skin roughness and wrinkle state measured using PRISMO after application of the cosmetic composition according to the present invention.

9 is a graph showing the results of measuring the skin density after application of the cosmetic composition according to the present invention.

10 is a graph showing the results of analysis of skin density improvement after application of the cosmetic composition according to the present invention for each age group.

FIG. 11 is a Dermascan image showing changes in skin density after application of the cosmetic composition according to the present invention. FIG.

FIG. 12 is a graph showing the results of measuring skin brightness after application of the cosmetic composition according to the present invention.

13 is a graph showing the results of analysis of skin brightness improvement after application of the cosmetic composition according to the present invention for each age group.

Claims (8)

An apple stem cell, a hibiscus seed extract and argan oil as an active ingredient, Wherein said stem cells are contained in an amount of 1.0 to 3.0% by weight based on the total weight of the composition, The hibiscus seed extract is contained in an amount of 0.5 to 1.0% by weight based on the total weight of the composition, Wherein the argan oil is contained in an amount of 0.5 to 1.0% by weight based on the total weight of the composition. delete delete delete The composition according to claim 1, wherein the composition further comprises at least one selected from the group consisting of oat seed extract, hyaluronic acid, chitosan, soybean extract, polyglyceryl-8 stearate, ceramide, phytosphingosine and yeast extract Wherein the anti-aging cosmetic composition comprises: An apple stem cell, a hibiscus seed extract, and argan oil as an active ingredient. An apple stem cell, a hibiscus seed extract, and argan oil as an active ingredient. An apple stem cell, a hibiscus seed extract, and argan oil as an active ingredient. The cosmetic composition for increasing skin luster or reducing skin roughness.

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