KR101039563B1 - 증폭 방법 - Google Patents

증폭 방법 Download PDF

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Publication number
KR101039563B1
KR101039563B1 KR1020037014191A KR20037014191A KR101039563B1 KR 101039563 B1 KR101039563 B1 KR 101039563B1 KR 1020037014191 A KR1020037014191 A KR 1020037014191A KR 20037014191 A KR20037014191 A KR 20037014191A KR 101039563 B1 KR101039563 B1 KR 101039563B1
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South Korea
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English (en)
Korean (ko)
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KR20040015216A (ko
Inventor
클라크던칸로이
빈센트수잔느패트리샤
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더 세크러터리 오브 스테이트 포 디펜스
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Publication of KR20040015216A publication Critical patent/KR20040015216A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/525—Phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
    • C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
    • C12Q2549/101—Hot start

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020037014191A 2001-04-30 2002-04-22 증폭 방법 Expired - Fee Related KR101039563B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0110501.4A GB0110501D0 (en) 2001-04-30 2001-04-30 Amplification process
GB0110501.4 2001-04-30
PCT/GB2002/001861 WO2002088387A2 (en) 2001-04-30 2002-04-22 Amplification process

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020097025792A Division KR20100005245A (ko) 2001-04-30 2002-04-22 핵산 증폭 반응 중에 비특이적 생산물의 생산을 억제하는 방법

Publications (2)

Publication Number Publication Date
KR20040015216A KR20040015216A (ko) 2004-02-18
KR101039563B1 true KR101039563B1 (ko) 2011-06-09

Family

ID=9913697

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020037014191A Expired - Fee Related KR101039563B1 (ko) 2001-04-30 2002-04-22 증폭 방법
KR1020097025792A Withdrawn KR20100005245A (ko) 2001-04-30 2002-04-22 핵산 증폭 반응 중에 비특이적 생산물의 생산을 억제하는 방법

Family Applications After (1)

Application Number Title Priority Date Filing Date
KR1020097025792A Withdrawn KR20100005245A (ko) 2001-04-30 2002-04-22 핵산 증폭 반응 중에 비특이적 생산물의 생산을 억제하는 방법

Country Status (14)

Country Link
US (2) US6951744B2 (https=)
EP (1) EP1390532B1 (https=)
JP (1) JP4177118B2 (https=)
KR (2) KR101039563B1 (https=)
CN (1) CN1318604C (https=)
AR (1) AR035238A1 (https=)
AT (1) ATE502118T1 (https=)
AU (1) AU2002249444B2 (https=)
CA (1) CA2445864C (https=)
DE (1) DE60239456D1 (https=)
GB (2) GB0110501D0 (https=)
NZ (1) NZ528919A (https=)
TW (1) TWI323284B (https=)
WO (1) WO2002088387A2 (https=)

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* Cited by examiner, † Cited by third party
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GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
CN1500887A (zh) * 2002-10-01 2004-06-02 ���µ�����ҵ��ʽ���� 引物伸长反应检测方法、碱基种类判别方法及其装置
EP1634965B1 (en) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Real time PCR with the addition of pyrophosphatase
US20060051796A1 (en) * 2004-09-09 2006-03-09 Inga Boell Real time PCR with the addition of pyrophosphatase
GB0502010D0 (en) * 2005-02-01 2005-03-09 Enigma Diagnostics Ltd Biochemical reagents and their uses
ATE502122T1 (de) 2006-06-01 2011-04-15 Trilink Biotechnologies Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation
KR101098764B1 (ko) 2007-10-29 2011-12-26 (주)바이오니아 안정화된 핫스타트 ｐｃｒ용 건조 조성물
EP2294076B1 (en) 2008-05-27 2017-03-08 TriLink BioTechnologies Chemically modified nucleoside 5'-triphosphates for thermally initiated replication of nucleic acid
CA2793970A1 (en) * 2010-04-30 2011-11-03 F. Hoffmann-La Roche Ag System and method for purification and use of inorganic pyrophosphatase from aquifex aeolicus
US20110312537A1 (en) * 2010-06-17 2011-12-22 Geneasys Pty Ltd Loc device for amplifying and detecting target nucleic acid sequences using electrochemiluminescent resonant energy transfer, linear probes with covalently attached primers
KR101870311B1 (ko) * 2012-03-09 2018-06-25 (주)바이오니아 핫스타트 역전사반응 또는 핫스타트 역전사 중합효소 연쇄반응용 조성물
JP6450308B2 (ja) 2012-06-14 2019-01-09 ライフ テクノロジーズ コーポレーション ポリメラーゼ連鎖反応（ｐｃｒ）のための新規の組成物、方法、およびキット
GB201301457D0 (en) 2013-01-28 2013-03-13 Fluorogenics Ltd Freeze-dried composition
JP6467829B2 (ja) * 2014-09-03 2019-02-13 東洋紡株式会社 改良されたｒｔ−ｐｃｒ反応
CN104862288B (zh) * 2015-05-08 2019-10-29 苏州大学 一种新型温度响应性无机焦磷酸酶偶联物的合成及其在增强聚合酶链式反应中的应用
WO2017121836A1 (en) 2016-01-15 2017-07-20 Thermo Fisher Scientific Baltics Uab Thermophilic dna polymerase mutants
EP3645710A1 (en) 2017-06-26 2020-05-06 Thermo Fisher Scientific Baltics Uab Thermophilic dna polymerase mutants
JP7234598B2 (ja) * 2018-01-26 2023-03-08 東ソー株式会社 核酸増幅試薬および当該試薬を用いた核酸増幅制御方法
CN113481180A (zh) * 2021-07-05 2021-10-08 吉林大学 碱性嗜热无机焦磷酸酶及其在增强聚合酶链式反应和合成udp-半乳糖反应中的应用
WO2025117716A1 (en) * 2023-11-30 2025-06-05 Watchmaker Genomics, Inc. Taq dna polymerase hotstart antibodies and methods of use thereof

Citations (1)

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Publication number Priority date Publication date Assignee Title
WO1994005797A1 (en) * 1992-09-01 1994-03-17 Vsevolod Kiselev Synthesis of dna molecules in vitro

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US4868103A (en) 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
CH670709A5 (https=) * 1986-04-24 1989-06-30 Univ Moskovsk
US5498523A (en) * 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
AU638246B2 (en) * 1989-04-12 1993-06-24 President And Fellows Of Harvard College Improved primer extension reactions
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
US5565339A (en) 1992-10-08 1996-10-15 Hoffmann-La Roche Inc. Compositions and methods for inhibiting dimerization of primers during storage of polymerase chain reaction reagents
US5491063A (en) 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5665551A (en) * 1995-09-13 1997-09-09 Roche Molecular Systems, Inc. Purified nucleic acid encoding a thermostable pyrophosphatase
US6291164B1 (en) * 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
GB9716052D0 (en) 1996-12-06 1997-10-01 Secr Defence Reaction vessels
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
GB9725237D0 (en) 1997-11-29 1998-01-28 Secr Defence Amplification system
GB9803382D0 (en) 1998-02-19 1998-04-15 Secr Defence Detection system
US6183998B1 (en) 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
KR100292883B1 (ko) * 1999-02-09 2001-06-15 박한오 피로포스페이트와 피로포스파타제를 포함하는 핫 스타트 ｐｃｒ 반응혼합물
DE60139762D1 (de) 2000-02-23 2009-10-15 Hope City Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren
KR20050005626A (ko) * 2003-07-07 2005-01-14 주식회사 포스코 코크스 오븐에서의 장입탄 밀도 향상방법

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
WO1994005797A1 (en) * 1992-09-01 1994-03-17 Vsevolod Kiselev Synthesis of dna molecules in vitro

Also Published As

Publication number Publication date
GB2377991B (en) 2004-01-28
WO2002088387A2 (en) 2002-11-07
WO2002088387A3 (en) 2003-12-11
US6951744B2 (en) 2005-10-04
US20060057617A1 (en) 2006-03-16
AR035238A1 (es) 2004-05-05
US20030049655A1 (en) 2003-03-13
AU2002249444B2 (en) 2007-07-12
EP1390532A2 (en) 2004-02-25
GB2377991A (en) 2003-01-29
KR20040015216A (ko) 2004-02-18
JP2004524853A (ja) 2004-08-19
CN1318604C (zh) 2007-05-30
GB0209052D0 (en) 2002-05-29
CA2445864A1 (en) 2002-11-07
ATE502118T1 (de) 2011-04-15
EP1390532B1 (en) 2011-03-16
KR20100005245A (ko) 2010-01-14
TWI323284B (en) 2010-04-11
GB0110501D0 (en) 2001-06-20
NZ528919A (en) 2005-07-29
JP4177118B2 (ja) 2008-11-05
CN1522305A (zh) 2004-08-18
DE60239456D1 (de) 2011-04-28
CA2445864C (en) 2011-08-02
US7449312B2 (en) 2008-11-11

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Ip right cessation event data comment text: Termination Category : DEFAULT_OF_REGISTRATION_FEE

Not in force date: 20170602

R18-X000 Changes to party contact information recorded

St.27 status event code: A-5-5-R10-R18-oth-X000

R18-X000 Changes to party contact information recorded

St.27 status event code: A-5-5-R10-R18-oth-X000