KR100460133B1 - 생열귀나무 추출물로부터 항산화활성이 우수한 카테킨의 생산방법 - Google Patents
생열귀나무 추출물로부터 항산화활성이 우수한 카테킨의 생산방법 Download PDFInfo
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- KR100460133B1 KR100460133B1 KR10-2000-0044372A KR20000044372A KR100460133B1 KR 100460133 B1 KR100460133 B1 KR 100460133B1 KR 20000044372 A KR20000044372 A KR 20000044372A KR 100460133 B1 KR100460133 B1 KR 100460133B1
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- Prior art keywords
- extract
- tree
- catechin
- activity
- antioxidant
- Prior art date
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Classifications
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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Abstract
Description
용매 | 추출수율(%, weight/weight) | |||
열매 | 잎 | 줄기 | 뿌리 | |
에탄올 | 3.1 | 15.2 | 3.7 | 7.5 |
메탄올 | 4.2 | 23.3 | 7.3 | 15.3 |
클로로포름 | 0.9 | 5.5 | 0.8 | 0.4 |
물 | 8.6 | 26.0 | 8.0 | 13.0 |
추출물 | 항산화 활성 [IC50: ㎍/ml]* | |||
에탄올 | 메탄올 | 클로르포름 | 믈 | |
열매 | 21.5 | 13.9 | 〉200.0 | 9.6 |
잎 | 11.5 | 6.4 | 〉200.0 | 4.8 |
줄기 | 5.6 | 3.9 | 21.2 | 5.6 |
뿌리 | 3.7 | 2.9 | 76.9 | 5.2 |
항산화제 | ||||
BHT | 5.4 | |||
비타민 C (Vitamin C) | 1.7 | |||
알파-토코페롤 (α-Tocopherol) | 4.2 |
분획물 | 항산화 활성 [IC50: μg/ml]* |
BHT | 5.4 |
비타민 C (Vitamin C) | 1.7 |
알파-토코페롤 (α-Tocopherol) | 4.2 |
메탄올 추출물 | 2.9 |
헥산 분획물 | >400.0 |
클로로포름 분획물 | 8.0 |
에틸아세테이트 분획물 | 1.7 |
부탄올 분획물 | 2.0 |
물 분획물 | 11.3 |
추출물 | % 함량, 평균±표준편차 | ||
메탄올 | 에탄올 | 물 | |
열매 | 0.0±0.0 | 0.0±0.0 | 0.0±0.0 |
잎 | 0.0±0.0 | 1.4±0.1 | 0.0±0.0 |
줄기 | 18.1±0.3 | 8.9±0.2 | 3.8±0.3 |
뿌리 | 25.0±1.5 | 13.3±1.1 | 0.0±0.0 |
에틸아세테이트분획물 | 33.4±0.2 | ||
부탄올 분획물 | 10.5±0.1 |
화합물 | 항산화 활성 [IC50: μg/ml]* |
화학식 1의 화합물 | 1.7 |
BHT | 5.4 |
비타민 C (Vitamin C) | 1.7 |
알파-토코페롤 (α-Tocopherol) | 4.2 |
카테친(Catechin) | 2.5 |
분획물 | 지질과산화 억제효과[IC50: ㎍/ml]* |
BHT | 0.33 |
비타민 C (Vitamin C) | 221 |
알파-토코페롤 (α-Tocopherol) | 162 |
메탄올 추출물 | 58 |
헥산 분획물 | 129 |
클로로포름 분획물 | 154 |
에틸아세테이트 분획물 | 25 |
부탄올 분획물 | 121 |
물 분획물 | >300 |
Claims (7)
- (1) 생열귀 나무의 줄기 또는 뿌리의 메탄올 추출물을 얻는 단계; (2) 상기 단계1에서 얻은 추출물의 에틸아세테이트 분획물을 얻는 단계; (3) 단계2의 에틸아세테이트 분획물에 대하여 클로로포름:메탄올의 혼합용매를 사용하여 실리카겔 컬럼 크로마토그래피를 수행하여 활성분획물을 얻는 단계; 및 (4) 단계3의 활성분획물에 메탄올을 용매로 사용하여 세파덱스 엘에이치 컬럼크로마토그래피를 수행하여 하기 화학식의 카테킨을 얻는 단계를 포함하는 항산화활성이 우수한 카테킨의 생산방법
- 삭제
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KR20160008862A (ko) | 2014-07-15 | 2016-01-25 | 주식회사 래디안 | 생열귀 에틸아세테이트 추출물을 유효성분으로 함유하는 피부 자극 완화, 피부 장벽 강화 및 염증 억제용 조성물 |
KR20190047983A (ko) | 2017-10-30 | 2019-05-09 | 주식회사 청진바이오텍 | 고순도 멜리틴, 진세노사이드 Rb1 및 Rg3, 및 생열귀나무 열매 추출물을 유효성분으로 함유하는 기능성 화장료 조성물 및 이의 제조방법 |
KR102605534B1 (ko) * | 2022-08-03 | 2023-11-24 | 박헌의 | 산삼배양근 배양 배지를 이용한 식용 달팽이용 사료 조성물 |
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KR100413927B1 (ko) * | 2000-09-22 | 2004-01-13 | 김석남 | 생열귀나무의 기능성 음료수 및 그 제조방법 |
KR100431275B1 (ko) * | 2001-07-19 | 2004-05-12 | 강원도 | 생열귀 농축액 캡슐이 함유된 음료의 제조방법 |
KR20040038243A (ko) * | 2002-10-31 | 2004-05-08 | (주)마이코스메틱 | 생열귀나무 추출물을 함유하는 항산화 또는 항노화용 피부화장료 조성물 |
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KR101043813B1 (ko) * | 2010-12-16 | 2011-06-27 | 주식회사 코스메카코리아 | 생열귀 추출물을 함유하는 화장료 조성물 |
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KR20230014156A (ko) * | 2021-07-21 | 2023-01-30 | 스노우화이트팩토리(주) | 생열귀나무 추출물을 유효성분으로 포함하는 항비만용 조성물 |
CN114796048B (zh) * | 2022-04-22 | 2023-11-03 | 宿迁市中医院 | 一种用于制备抗氧化剂的中药萃取物的制备及其应用 |
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Cited By (3)
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KR20160008862A (ko) | 2014-07-15 | 2016-01-25 | 주식회사 래디안 | 생열귀 에틸아세테이트 추출물을 유효성분으로 함유하는 피부 자극 완화, 피부 장벽 강화 및 염증 억제용 조성물 |
KR20190047983A (ko) | 2017-10-30 | 2019-05-09 | 주식회사 청진바이오텍 | 고순도 멜리틴, 진세노사이드 Rb1 및 Rg3, 및 생열귀나무 열매 추출물을 유효성분으로 함유하는 기능성 화장료 조성물 및 이의 제조방법 |
KR102605534B1 (ko) * | 2022-08-03 | 2023-11-24 | 박헌의 | 산삼배양근 배양 배지를 이용한 식용 달팽이용 사료 조성물 |
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