JPWO2020127417A5 - - Google Patents
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- Publication number
- JPWO2020127417A5 JPWO2020127417A5 JP2021535101A JP2021535101A JPWO2020127417A5 JP WO2020127417 A5 JPWO2020127417 A5 JP WO2020127417A5 JP 2021535101 A JP2021535101 A JP 2021535101A JP 2021535101 A JP2021535101 A JP 2021535101A JP WO2020127417 A5 JPWO2020127417 A5 JP WO2020127417A5
- Authority
- JP
- Japan
- Prior art keywords
- amino acid
- seq
- fucosyllactose
- acid sequence
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- AUNPEJDACLEKSC-ZAYDSPBTSA-N 3-Fucosyllactose Chemical compound O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@H](OC(O)[C@H](O)[C@H]2O)CO)O[C@H](CO)[C@@H]1O AUNPEJDACLEKSC-ZAYDSPBTSA-N 0.000 claims description 68
- 210000004027 cells Anatomy 0.000 claims description 67
- GUBGYTABKSRVRQ-UUNJERMWSA-N Lactose Natural products O([C@@H]1[C@H](O)[C@H](O)[C@H](O)O[C@@H]1CO)[C@H]1[C@@H](O)[C@@H](O)[C@H](O)[C@H](CO)O1 GUBGYTABKSRVRQ-UUNJERMWSA-N 0.000 claims description 65
- GUBGYTABKSRVRQ-XLOQQCSPSA-N lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 65
- 239000008101 lactose Substances 0.000 claims description 65
- 229920001184 polypeptide Polymers 0.000 claims description 63
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 60
- 108010001671 galactoside 3-fucosyltransferase Proteins 0.000 claims description 38
- 239000000758 substrate Substances 0.000 claims description 37
- 150000001413 amino acids Chemical class 0.000 claims description 35
- 239000001963 growth media Substances 0.000 claims description 31
- 238000004519 manufacturing process Methods 0.000 claims description 31
- 230000000694 effects Effects 0.000 claims description 30
- LQEBEXMHBLQMDB-UHFFFAOYSA-N [[5-(2-amino-6-oxo-3H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] (3,4,5-trihydroxy-6-methyloxan-2-yl) hydrogen phosphate Chemical compound OC1C(O)C(O)C(C)OC1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C3=C(C(N=C(N)N3)=O)N=C2)O1 LQEBEXMHBLQMDB-UHFFFAOYSA-N 0.000 claims description 29
- 230000027455 binding Effects 0.000 claims description 20
- 239000012527 feed solution Substances 0.000 claims description 16
- OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 13
- 244000005700 microbiome Species 0.000 claims description 11
- 241000588724 Escherichia coli Species 0.000 claims description 9
- 229920000023 polynucleotide Polymers 0.000 claims description 9
- 239000002157 polynucleotide Substances 0.000 claims description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 8
- 238000000855 fermentation Methods 0.000 claims description 8
- 230000004151 fermentation Effects 0.000 claims description 8
- 238000000746 purification Methods 0.000 claims description 8
- 125000001433 C-terminal amino-acid group Chemical group 0.000 claims description 7
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 claims description 7
- 210000004897 N-terminal region Anatomy 0.000 claims description 7
- GUBGYTABKSRVRQ-QKKXKWKRSA-N lactose group Chemical group OC1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@@H](O)[C@H](O2)CO)[C@H](O1)CO GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 241000196324 Embryophyta Species 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 238000000108 ultra-filtration Methods 0.000 claims description 5
- 241001646716 Escherichia coli K-12 Species 0.000 claims description 4
- 241000660147 Escherichia coli str. K-12 substr. MG1655 Species 0.000 claims description 4
- 241000238631 Hexapoda Species 0.000 claims description 4
- 229920001850 Nucleic acid sequence Polymers 0.000 claims description 4
- 238000007792 addition Methods 0.000 claims description 4
- 230000001580 bacterial Effects 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 239000002609 media Substances 0.000 claims description 4
- 238000001728 nano-filtration Methods 0.000 claims description 4
- 150000007523 nucleic acids Chemical group 0.000 claims description 4
- 239000000243 solution Substances 0.000 claims description 4
- 238000003786 synthesis reaction Methods 0.000 claims description 4
- 230000002194 synthesizing Effects 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 3
- 239000003054 catalyst Substances 0.000 claims description 3
- 241000251468 Actinopterygii Species 0.000 claims description 2
- 240000002791 Brassica napus Species 0.000 claims description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 2
- 210000004671 Cell-Free System Anatomy 0.000 claims description 2
- 229920000742 Cotton Polymers 0.000 claims description 2
- 241000602080 Dracaena fragrans Species 0.000 claims description 2
- SHZGCJCMOBCMKK-DHVFOXMCSA-N Fucose Chemical group C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 claims description 2
- 241000233866 Fungi Species 0.000 claims description 2
- 240000007842 Glycine max Species 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- 240000006962 Gossypium hirsutum Species 0.000 claims description 2
- 230000036740 Metabolism Effects 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 240000008042 Zea mays Species 0.000 claims description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 2
- 238000001042 affinity chromatography Methods 0.000 claims description 2
- 210000004102 animal cell Anatomy 0.000 claims description 2
- 239000003610 charcoal Substances 0.000 claims description 2
- 238000005352 clarification Methods 0.000 claims description 2
- 238000002425 crystallisation Methods 0.000 claims description 2
- 230000005712 crystallization Effects 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- 238000001704 evaporation Methods 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 238000004108 freeze drying Methods 0.000 claims description 2
- 230000002538 fungal Effects 0.000 claims description 2
- 238000002523 gelfiltration Methods 0.000 claims description 2
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims description 2
- 238000005342 ion exchange Methods 0.000 claims description 2
- 238000004255 ion exchange chromatography Methods 0.000 claims description 2
- 238000004246 ligand exchange chromatography Methods 0.000 claims description 2
- 235000009973 maize Nutrition 0.000 claims description 2
- 230000004060 metabolic process Effects 0.000 claims description 2
- 230000035786 metabolism Effects 0.000 claims description 2
- 238000001471 micro-filtration Methods 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 claims description 2
- 238000001556 precipitation Methods 0.000 claims description 2
- 230000001105 regulatory Effects 0.000 claims description 2
- 238000001223 reverse osmosis Methods 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- RTVRUWIBAVHRQX-PMEZUWKYSA-N Fucosyllactose Chemical compound C([C@H]1O[C@@H]([C@H]([C@@H](O[C@@H]2[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)[C@@H]1O)O)OC)O[C@H]1OC[C@@H](O)[C@H](O)[C@@H]1O RTVRUWIBAVHRQX-PMEZUWKYSA-N 0.000 claims 1
- 108020004705 Codon Proteins 0.000 description 1
- 101700006177 FUT2 Proteins 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 101700047062 pgtA Proteins 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP18213728.1 | 2018-12-18 | ||
| EP18213728 | 2018-12-18 | ||
| PCT/EP2019/085841 WO2020127417A2 (en) | 2018-12-18 | 2019-12-18 | PRODUCTION OF 3-FUCOSYLLACTOSE AND LACTOSE CONVERTING α-1,3-FUCOSYLTRANSFERASE ENZYMES |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2022514743A JP2022514743A (ja) | 2022-02-15 |
| JPWO2020127417A5 true JPWO2020127417A5 (zh) | 2022-12-26 |
Family
ID=64745987
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021535101A Pending JP2022514743A (ja) | 2018-12-18 | 2019-12-18 | ３－フコシルラクトースの産生およびラクトース変換α－１，３－フコシルトランスフェラーゼ酵素 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20220002773A1 (zh) |
| EP (1) | EP3898642A2 (zh) |
| JP (1) | JP2022514743A (zh) |
| KR (1) | KR20210107736A (zh) |
| CN (1) | CN113195509A (zh) |
| AU (1) | AU2019409833A1 (zh) |
| BR (1) | BR112021011298A2 (zh) |
| CA (1) | CA3121848A1 (zh) |
| SG (1) | SG11202106291RA (zh) |
| WO (1) | WO2020127417A2 (zh) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114438001B (zh) * | 2020-11-05 | 2022-11-15 | 中国科学院上海高等研究院 | 生产3-岩藻糖基乳糖的重组枯草芽孢杆菌及其构建方法和应用 |
| CN112662604B (zh) * | 2020-12-29 | 2023-10-20 | 量子高科(广东)生物有限公司 | 一种合成3-岩藻糖基乳糖的重组大肠杆菌及其构建方法 |
| CN113648405B (zh) * | 2021-08-19 | 2023-06-02 | 重庆医科大学 | 一种口服重组幽门螺杆菌蛋白疫苗纳米粒及其制备方法 |
| WO2023110995A1 (en) * | 2021-12-14 | 2023-06-22 | Inbiose N.V. | Production of alpha-1,3-fucosylated compounds |
| WO2023110994A1 (en) * | 2021-12-14 | 2023-06-22 | Inbiose N.V. | Production of alpha-1,4-fucosylated compounds |
| CN115287273A (zh) * | 2022-06-30 | 2022-11-04 | 华熙生物科技股份有限公司 | 一种1，2-岩藻糖基转移酶及其融合蛋白和编码基因 |
| CN115058465A (zh) * | 2022-06-30 | 2022-09-16 | 山东大学 | 一种岩藻糖基化软骨素及其制备方法和应用 |
| KR20240017427A (ko) * | 2022-07-29 | 2024-02-08 | 주식회사 진켐 | 3-푸코실락토스 생산용 유전자 조작 미생물 및 이를 이용한 방법 |
| WO2024089131A1 (en) | 2022-10-25 | 2024-05-02 | Inbiose N.V. | Saccharide importers for lacto-n-triose |
| CN116948928B (zh) * | 2023-06-02 | 2024-06-18 | 虹摹生物科技(上海)有限公司 | 种子培养基及无抗生素、无iptg诱导剂的2’-岩藻糖基乳糖的发酵生产方法 |
| CN116425810B (zh) * | 2023-06-14 | 2023-08-11 | 山东合成远景生物科技有限公司 | 一种混合液中3-岩藻糖基乳糖的纯化方法 |
| CN118047820A (zh) * | 2024-04-16 | 2024-05-17 | 北京三元食品股份有限公司 | 一种3-岩藻糖基乳糖的制备方法及制备的标准品 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6399337B1 (en) | 1997-06-06 | 2002-06-04 | The Governors Of The University Of Alberta | α1,3-fucosyltransferase |
| NZ605093A (en) * | 2010-07-12 | 2014-05-30 | Univ Gent | Metabolically engineered organisms for the production of added value bio-products |
| EP2439264B1 (en) | 2010-10-11 | 2015-06-03 | Jennewein Biotechnologie GmbH | Novel fucosyltransferases and their applications |
| EP3191499A4 (en) * | 2014-09-09 | 2018-06-06 | Glycosyn LLC | Alpha (1,3) fucosyltransferases for use in the production of fucosylated oligosaccharides |
| JP6765372B2 (ja) | 2014-11-14 | 2020-10-07 | インビオース エヌ．ヴェ．Ｉｎｂｉｏｓｅ Ｎ．Ｖ． | ラクトース死滅に耐性である変異体微生物 |
| CN108103039B (zh) * | 2016-11-25 | 2021-10-22 | 上海交通大学 | 一组岩藻糖基转移酶突变体及其筛选方法和应用 |
-
2019
- 2019-12-18 US US17/295,688 patent/US20220002773A1/en active Pending
- 2019-12-18 KR KR1020217022575A patent/KR20210107736A/ko unknown
- 2019-12-18 AU AU2019409833A patent/AU2019409833A1/en active Pending
- 2019-12-18 CA CA3121848A patent/CA3121848A1/en active Pending
- 2019-12-18 JP JP2021535101A patent/JP2022514743A/ja active Pending
- 2019-12-18 SG SG11202106291RA patent/SG11202106291RA/en unknown
- 2019-12-18 BR BR112021011298A patent/BR112021011298A2/pt unknown
- 2019-12-18 EP EP19832061.6A patent/EP3898642A2/en active Pending
- 2019-12-18 WO PCT/EP2019/085841 patent/WO2020127417A2/en unknown
- 2019-12-18 CN CN201980083180.5A patent/CN113195509A/zh active Pending
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