JPWO2014129292A1 - 生体分析デバイスおよび生体分子分析装置 - Google Patents
生体分析デバイスおよび生体分子分析装置 Download PDFInfo
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Abstract
Description
102 溶液
103 磁気微粒子
104 磁場
201 平面基板
202 溶液
203 磁気微粒子
204 磁場
301 スライドガラス
302 カバーガラス
303 磁気微粒子
304 溶液
801 支持基板
802 カバー材
803 溶液
804 送液管
805 排出管
806 磁場
901 抗原
902 抗体
903 磁気微粒子
904 蛍光色素
905 二次抗体
906 平面基板
907 磁場
1001 試料核酸断片
1002 磁気微粒子
1003 試料の相補配列断片
1004 蛍光色素
1005 アダプタの相補配列断片
1006 支持基板
1007 磁場
1101 支持基板
1102 可動ステージ
1103 磁石ホルダ
1104 デバイス
1105 送液管
1106 排出管
1107 磁場発生装置
1108 励起光源
1109 励起フィルタ
1110 レンズ
1111 ダイクロイックミラー
1112 対物レンズ
1113 結像レンズ
1114 CCDカメラ
1115 吸収フィルタ
1116 送液ポンプ
Claims (14)
- 上下を2枚の親水性の高い平面基板で挟まれた溶液槽を有し、一方の平面基板側に磁力のオンオフ、あるいは磁力の強弱の切り替えが可能な磁場発生手段を備え、当該磁場発生手段によって溶液槽内の磁気微粒子を基板固定することを特徴とする生体分子分析用デバイス。
- 前記平面基板間の幅として100μm以下であることを特徴とする請求項1記載の生体分子分析用デバイス。
- 前記磁気微粒子として粒径0.02〜1μmの常磁性磁気微粒子を用いる請求項1または請求項2記載の生体分子分析用デバイス。
- 前記平面基板の表面が蒸留水に対する接触角が30°以下であることを特徴とする請求項1〜3のいずれか1項記載の生体分子分析用デバイス。
- 上下を2枚の濡れ性の高い前記平面基板から得られる毛細管現象によって、溶液槽内に溶液を導入することを特徴とする請求項1〜4のいずれか1項記載の生体分子分析用デバイス。
- 前記磁場発生装置として、
電磁石、可動式の永久磁石、可動式の磁場遮蔽物が組み合わさった電磁石、可動式の磁場遮蔽物が組み合わさった永久磁石のうちいずれかを用いることを特徴とする請求項1〜5のいずれか1項記載の生体分子分析用デバイス。 - 溶液槽の導入口と排出口に疎水性表面の送液管が接続されて送液手段を有し、流路構造を持つことを特徴とする請求項1〜6のいずれか1項記載の生体分子分析用デバイス。
- 前記請求項1において、
前記生体分子が抗原分子であり、磁気微粒子による捕捉が抗原抗体反応によって実施されることを特徴とする請求項1〜7のいずれか1項記載の生体分子分析用デバイス。 - 前記請求項1において、
前記生体分子が核酸分子であり、磁気微粒子による捕捉がハイブリダイゼーションによって実施されることを特徴とする請求項1〜8のいずれか1項記載の生体分子分析用デバイス。 - 請求項1〜9のいずれか1項記載の生体試料分析用デバイスと、蛍光色素を励起する手段と、蛍光を検出する手段とが一体化した生体分子分析装置。
- 検出器側の平面基板として、ガラス、あるいは親水化処理を行った光学用ポリマーを使用することを特徴とした請求項10記載の生体分子分析装置。
- 前記蛍光色素を励起して、蛍光を検出する手段として、落射型光学顕微鏡を用いることを特徴とする請求項10または請求項11に記載の生体分子分析装置。
- 前記生体分子を蛍光色素で標識して1分子ずつカウンティングすることを特徴とした請求項10〜12のいずれか1項に記載の生体分子分析装置。
- 磁力がオフ、あるいは磁力が弱の状態で溶液槽内に磁気微粒子溶液を導入し、その後に磁力がオン、あるいは磁力が強にしてから蛍光色素を観察する順で行われることを特徴とする請求項10〜13のいずれか1項に記載の生体分子分析装置。
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US10801959B2 (en) * | 2015-06-18 | 2020-10-13 | Konica Minolta, Inc. | Objective biological substance analysis device, analysis system, analysis method, and recording medium storing computer readable analysis program |
WO2017057136A1 (ja) * | 2015-09-29 | 2017-04-06 | コニカミノルタ株式会社 | 表面プラズモン励起増強蛍光分光測定方法、および測定用キット |
CN109154561B (zh) * | 2016-04-28 | 2021-04-13 | 国立研究开发法人产业技术综合研究所 | 光学检测方法及光学检测装置 |
CN105891493B (zh) * | 2016-06-01 | 2017-10-24 | 章健 | 一种荧光检测装置及其检测方法 |
CN106053786B (zh) * | 2016-06-01 | 2017-12-12 | 章健 | 一种时间分辨荧光标记物的荧光检测方法及其装置 |
DE102017112612A1 (de) * | 2016-06-10 | 2017-12-14 | Hitachi High-Tech Science Corporation | Flüssigkeitschromatograph und Verfahren zum Korrigieren von Schwankungen eines Detektorausgabewerts des Flüssigkeitschromatographen |
US11408028B2 (en) | 2017-02-08 | 2022-08-09 | Essenlix Corporation | Nucleic acid hybridization assay |
JP6905838B2 (ja) * | 2017-03-03 | 2021-07-21 | 株式会社日立ハイテク | 生体分子分析装置および生体分子分析方法 |
US11712177B2 (en) | 2019-08-12 | 2023-08-01 | Essenlix Corporation | Assay with textured surface |
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US6294342B1 (en) * | 1999-09-29 | 2001-09-25 | Abbott Laboratories | Magnetically assisted binding assays utilizing a magnetically responsive reagent |
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JP5301894B2 (ja) * | 2008-06-27 | 2013-09-25 | 富士フイルム株式会社 | 検出方法 |
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CN101458251B (zh) * | 2009-01-04 | 2013-02-27 | 东南大学 | 高通量检测生物大分子的磁性颗粒微阵列装置及其使用方法 |
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