JPS6147186A - インフルエンザウイルスの精製方法 - Google Patents
インフルエンザウイルスの精製方法Info
- Publication number
- JPS6147186A JPS6147186A JP59167324A JP16732484A JPS6147186A JP S6147186 A JPS6147186 A JP S6147186A JP 59167324 A JP59167324 A JP 59167324A JP 16732484 A JP16732484 A JP 16732484A JP S6147186 A JPS6147186 A JP S6147186A
- Authority
- JP
- Japan
- Prior art keywords
- influenza virus
- sulfate
- crosslinked
- cellulose
- item
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000712461 unidentified influenza virus Species 0.000 title claims abstract description 57
- 238000000034 method Methods 0.000 title claims description 41
- 229920002678 cellulose Polymers 0.000 claims abstract description 34
- 239000001913 cellulose Substances 0.000 claims abstract description 33
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims abstract description 22
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 20
- 239000005017 polysaccharide Substances 0.000 claims abstract description 20
- -1 polysaccharide sulfate Chemical class 0.000 claims abstract description 18
- 238000004440 column chromatography Methods 0.000 claims abstract description 7
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000000427 antigen Substances 0.000 claims description 21
- 102000036639 antigens Human genes 0.000 claims description 21
- 108091007433 antigens Proteins 0.000 claims description 21
- 241000700605 Viruses Species 0.000 claims description 15
- 239000012530 fluid Substances 0.000 claims description 13
- 238000004587 chromatography analysis Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 229920000936 Agarose Polymers 0.000 claims description 6
- 241000287828 Gallus gallus Species 0.000 claims description 5
- 229960000633 dextran sulfate Drugs 0.000 claims description 5
- 235000013601 eggs Nutrition 0.000 claims description 3
- 241000271566 Aves Species 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 claims description 2
- 239000012930 cell culture fluid Substances 0.000 claims description 2
- 241000282412 Homo Species 0.000 claims 1
- 239000000243 solution Substances 0.000 abstract description 11
- 239000007853 buffer solution Substances 0.000 abstract description 7
- 238000011067 equilibration Methods 0.000 abstract description 7
- 150000004676 glycans Chemical class 0.000 abstract description 7
- 238000004132 cross linking Methods 0.000 abstract description 2
- 239000003431 cross linking reagent Substances 0.000 abstract description 2
- 239000003463 adsorbent Substances 0.000 abstract 2
- 230000001180 sulfating effect Effects 0.000 abstract 2
- 239000013078 crystal Substances 0.000 abstract 1
- 239000008055 phosphate buffer solution Substances 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 43
- 235000010980 cellulose Nutrition 0.000 description 24
- 239000011780 sodium chloride Substances 0.000 description 22
- 239000000499 gel Substances 0.000 description 20
- 239000008363 phosphate buffer Substances 0.000 description 19
- 238000000746 purification Methods 0.000 description 15
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 206010022000 influenza Diseases 0.000 description 10
- 229960005486 vaccine Drugs 0.000 description 9
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 8
- TZCXTZWJZNENPQ-UHFFFAOYSA-L barium sulfate Chemical compound [Ba+2].[O-]S([O-])(=O)=O TZCXTZWJZNENPQ-UHFFFAOYSA-L 0.000 description 8
- 239000000203 mixture Substances 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 6
- 239000002953 phosphate buffered saline Substances 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- AXISYYRBXTVTFY-UHFFFAOYSA-N Isopropyl tetradecanoate Chemical compound CCCCCCCCCCCCCC(=O)OC(C)C AXISYYRBXTVTFY-UHFFFAOYSA-N 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 238000001914 filtration Methods 0.000 description 5
- 229960003971 influenza vaccine Drugs 0.000 description 5
- KEQGZUUPPQEDPF-UHFFFAOYSA-N 1,3-dichloro-5,5-dimethylimidazolidine-2,4-dione Chemical compound CC1(C)N(Cl)C(=O)N(Cl)C1=O KEQGZUUPPQEDPF-UHFFFAOYSA-N 0.000 description 4
- 238000007792 addition Methods 0.000 description 4
- 235000013330 chicken meat Nutrition 0.000 description 4
- XTHPWXDJESJLNJ-UHFFFAOYSA-N chlorosulfonic acid Substances OS(Cl)(=O)=O XTHPWXDJESJLNJ-UHFFFAOYSA-N 0.000 description 4
- 230000034994 death Effects 0.000 description 4
- 231100000517 death Toxicity 0.000 description 4
- 238000010828 elution Methods 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- XZEUAXYWNKYKPL-URLMMPGGSA-N ormeloxifene Chemical compound C1([C@@H]2[C@H](C3=CC=C(C=C3OC2(C)C)OC)C=2C=CC(OCCN3CCCC3)=CC=2)=CC=CC=C1 XZEUAXYWNKYKPL-URLMMPGGSA-N 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 229920002307 Dextran Polymers 0.000 description 2
- 108010067390 Viral Proteins Proteins 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000000432 density-gradient centrifugation Methods 0.000 description 2
- 229960002086 dextran Drugs 0.000 description 2
- 230000032050 esterification Effects 0.000 description 2
- 238000005886 esterification reaction Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- UFWWAKOWSBGGCP-UHFFFAOYSA-N pyridine;sulfurochloridic acid Chemical compound OS(Cl)(=O)=O.C1=CC=NC=C1 UFWWAKOWSBGGCP-UHFFFAOYSA-N 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 125000001174 sulfone group Chemical group 0.000 description 2
- AKEJUJNQAAGONA-UHFFFAOYSA-N sulfur trioxide Inorganic materials O=S(=O)=O AKEJUJNQAAGONA-UHFFFAOYSA-N 0.000 description 2
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241000272517 Anseriformes Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- PTHCMJGKKRQCBF-UHFFFAOYSA-N Cellulose, microcrystalline Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC)C(CO)O1 PTHCMJGKKRQCBF-UHFFFAOYSA-N 0.000 description 1
- 229920000832 Cutin Polymers 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 241000712431 Influenza A virus Species 0.000 description 1
- 241000713196 Influenza B virus Species 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 101710194807 Protective antigen Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 210000001643 allantois Anatomy 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 210000003000 inclusion body Anatomy 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 238000000464 low-speed centrifugation Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000012798 spherical particle Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- YBBRCQOCSYXUOC-UHFFFAOYSA-N sulfuryl dichloride Chemical compound ClS(Cl)(=O)=O YBBRCQOCSYXUOC-UHFFFAOYSA-N 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 239000005723 virus inoculator Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A61K39/145—Orthomyxoviridae, e.g. influenza virus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16151—Methods of production or purification of viral material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Public Health (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Pulmonology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Detergent Compositions (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59167324A JPS6147186A (ja) | 1984-08-09 | 1984-08-09 | インフルエンザウイルスの精製方法 |
| CA000488281A CA1251399A (en) | 1984-08-09 | 1985-08-08 | Method for purification of influenza virus |
| EP85110012A EP0171086B1 (en) | 1984-08-09 | 1985-08-08 | A method for purification of influenza virus |
| DE8585110012T DE3578613D1 (de) | 1984-08-09 | 1985-08-08 | Verfahren zur reinigung des influenzavirus. |
| AT85110012T ATE54468T1 (de) | 1984-08-09 | 1985-08-08 | Verfahren zur reinigung des influenzavirus. |
| US06/764,128 US4724210A (en) | 1984-08-09 | 1985-08-09 | Method for purification of influenza virus |
| KR1019850005760A KR920009733B1 (ko) | 1984-08-09 | 1985-08-09 | 인플루엔자 바이러스의 정제방법 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59167324A JPS6147186A (ja) | 1984-08-09 | 1984-08-09 | インフルエンザウイルスの精製方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6147186A true JPS6147186A (ja) | 1986-03-07 |
| JPS6230752B2 JPS6230752B2 (enExample) | 1987-07-03 |
Family
ID=15847633
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59167324A Granted JPS6147186A (ja) | 1984-08-09 | 1984-08-09 | インフルエンザウイルスの精製方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US4724210A (enExample) |
| EP (1) | EP0171086B1 (enExample) |
| JP (1) | JPS6147186A (enExample) |
| KR (1) | KR920009733B1 (enExample) |
| AT (1) | ATE54468T1 (enExample) |
| CA (1) | CA1251399A (enExample) |
| DE (1) | DE3578613D1 (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009042074A (ja) * | 2007-08-09 | 2009-02-26 | Denka Seiken Co Ltd | 吸着担体及び吸着担体の製造方法 |
| EP2266675A2 (en) | 2009-06-25 | 2010-12-29 | Chisso Corporation | Chromatography medium, preparation method of the same, and method for producing virus vaccine using the chromatography medium |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5215739A (en) * | 1989-04-05 | 1993-06-01 | Toko Yakuhin Kogyo Kabushiki Kaisha | Spray gel base and spray gel preparation using thereof |
| US5158761A (en) * | 1989-04-05 | 1992-10-27 | Toko Yakuhin Kogyo Kabushiki Kaisha | Spray gel base and spray gel preparation using thereof |
| FR2667622B1 (fr) * | 1990-10-08 | 1994-10-07 | Kaysersberg Sa | Montisse lie hydrauliquement et son procede de fabrication. |
| IT1248075B (it) * | 1991-06-18 | 1995-01-05 | Sclavo Spa | Processo per la purificazione del virus dell'epatite a (hav), virus cosi` purificato e composizioni vaccinali che lo contengono. |
| US5447859A (en) * | 1993-07-16 | 1995-09-05 | Viagene | Method for the purification or removal of retroviruses using sulfated cellulose |
| EP1010981A1 (de) * | 1998-12-18 | 2000-06-21 | Diagor GmbH | Konjugate zum Nachweis von Viren und deren Verwendung |
| JP2002142770A (ja) | 2000-11-08 | 2002-05-21 | Dnavec Research Inc | 循環系への遺伝子送達用パラミクソウイルスベクター |
| PL354822A1 (en) * | 2002-06-30 | 2004-01-12 | Instytut Immunologii i Terapii Doświadczalnej PANwe Wrocławiu | Method of receiving purified bacteriophage preparations and new applications of polysaccharide or its esterificated derivative |
| AU2004249199B2 (en) * | 2003-06-18 | 2008-07-24 | Onyx Pharmaceuticals, Inc. | Method for purifying virus |
| ATE478137T1 (de) * | 2003-06-20 | 2010-09-15 | Microbix Biosystems Inc | Verbesserungen bei der virusproduktion |
| CA2544786A1 (en) | 2003-11-04 | 2005-05-12 | Dnavec Research Inc. | Method for producing gene transferred dendritic cells |
| EP1916298B1 (en) | 2005-06-14 | 2011-12-28 | Dnavec Corporation | Methods for producing monoclonal antibodies |
| US8877187B2 (en) * | 2005-07-25 | 2014-11-04 | Avianax, Llc | Therapeutic antibodies for treatment and prophylaxis of transmittable viral diseases |
| US20070065452A1 (en) * | 2005-07-25 | 2007-03-22 | Avianax | Therapeutic antibodies for treatment and prophylaxis of transmittable viral diseases |
| EP1808697A1 (en) * | 2006-01-13 | 2007-07-18 | Novartis Vaccines and Diagnostics, Inc. | Use of an ion exchange matrix for determining the concentration of virus particles and/or virus antigens |
| JPWO2007083644A1 (ja) | 2006-01-17 | 2009-06-11 | ディナベック株式会社 | 新規タンパク質発現系 |
| US20100297604A1 (en) * | 2006-06-09 | 2010-11-25 | Xing-Xiang Li | Methods and reagents for virus isolation and detection |
| EP1878791A1 (en) * | 2006-07-11 | 2008-01-16 | Bia Separations D.O.O. | Method for influenza virus purification |
| WO2008007581A1 (en) | 2006-07-13 | 2008-01-17 | Dnavec Corporation | Non-replicating paramyxoviridae virus vector |
| WO2008039136A1 (en) * | 2006-09-29 | 2008-04-03 | Ge Healthcare Bio-Sciences Ab | Separation matrix for viral purification |
| EP1982727A1 (en) | 2007-04-17 | 2008-10-22 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Method for purification of viral proteins |
| EP2143794A1 (en) | 2007-04-27 | 2010-01-13 | Kyushu University, National University Corporation | Viral vector for gene therapy |
| EP2173866A2 (en) * | 2007-06-29 | 2010-04-14 | Avianax, LLC | Vaccine production for pathogenic bird viral diseases |
| BR112012026095A2 (pt) | 2010-04-14 | 2015-09-15 | Emd Millipore Corp | métodos de produção de estoques de vírus de alta pureza e altos títulos e métodos de uso dos mesmos. |
| CN104968403A (zh) | 2012-09-17 | 2015-10-07 | 格雷斯公司 | 色谱介质和装置 |
| CN105518059B (zh) * | 2013-09-02 | 2019-01-01 | 捷恩智株式会社 | 多孔性纤维素粒子的制造方法、多孔性纤维素粒子及色谱法用填充剂 |
| DE102013017014B4 (de) | 2013-10-14 | 2017-03-30 | Sartorius Stedim Biotech Gmbh | Sulfatierte Cellulosehydrat-Membran, Verfahren zur ihrer Herstellung und Verwendung der Membran als Adsorptionsmembran für die Virenaufreinigung |
| ES2929099T3 (es) | 2014-05-02 | 2022-11-24 | Grace W R & Co | Material de soporte funcionalizado y métodos de fabricación y uso de material de soporte funcionalizado |
| EP2966093A1 (en) | 2014-07-07 | 2016-01-13 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Process for the preparation of magnetic sulfated cellulose particles, magnetic sulfated cellulose particles and their use |
| PL3302784T3 (pl) | 2015-06-05 | 2022-01-17 | W.R. Grace & Co.-Conn. | Adsorbentowe środki klarujące do bioprzetwarzania oraz sposoby ich wytwarzania i stosowania |
| US10626376B2 (en) | 2016-11-14 | 2020-04-21 | St. Jude Children's Research Hospital | Method for isolating and purifying adeno-associated virus particles using salt |
| CN106622159B (zh) * | 2016-12-22 | 2019-06-14 | 苏州楚博生物技术有限公司 | 用于蛋白药物纯化的亲和层析介质 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL131092C (enExample) * | ||||
| US3105012A (en) * | 1961-10-19 | 1963-09-24 | Parke Davis & Co | Antigen products and means for producing the same |
| BE662899A (enExample) * | 1964-04-22 | |||
| US3547779A (en) * | 1968-12-18 | 1970-12-15 | Merck & Co Inc | Process for producing purified concentrated influenza virus |
| SE356735B (enExample) * | 1970-12-02 | 1973-06-04 | Svenska Cellulosa Ab | |
| US3842062A (en) * | 1972-09-27 | 1974-10-15 | Du Pont | Water-soluble complexes of protein and anionic polyelectrolytes such as sodium carboxymethyl cellulose |
| US3920625A (en) * | 1973-06-19 | 1975-11-18 | Kabi Ab | Isolation of coagulation factors from biological material using cross linked sulfated, sulfonated carbohydrates |
| US4160018A (en) * | 1973-06-25 | 1979-07-03 | Bjorklund Knut B | Stabilized cancer associated polypeptide antigen and preparation thereof |
| IL49752A (en) * | 1975-07-09 | 1979-07-25 | Kabi Ab | Compositions having affinity for hepatitis virus and method for hepatitis virus removal or concentration |
| SE420977B (sv) * | 1976-03-18 | 1981-11-16 | Kabi Ab | Forfarande for rening och isolering av hepatitvirus for vaccinframstellning |
| US4181713A (en) * | 1978-10-30 | 1980-01-01 | Merck & Co., Inc. | Isolation of HBs Ag |
| DE3014189A1 (de) * | 1980-04-14 | 1981-10-15 | Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg | Verfahren zur herstellung eines immunogenen membranproteinaggregats von influenza-, parainfluenza- und rhabdo-viren |
| JPS57136528A (en) * | 1981-02-09 | 1982-08-23 | Hayashibara Biochem Lab Inc | Preparation of viral vaccine |
| US4434093A (en) * | 1982-07-26 | 1984-02-28 | Ortho Diagnostic Systems Inc. | Methods for preparation of HBs Ag free gamma globulins |
| US4515714A (en) * | 1983-03-09 | 1985-05-07 | Juridicial Foundation, The Chemo-Semo-Sero-Therapeutic Research Institute | Method for purification of hepatitis B virus surface antigen |
-
1984
- 1984-08-09 JP JP59167324A patent/JPS6147186A/ja active Granted
-
1985
- 1985-08-08 DE DE8585110012T patent/DE3578613D1/de not_active Expired - Lifetime
- 1985-08-08 EP EP85110012A patent/EP0171086B1/en not_active Expired - Lifetime
- 1985-08-08 AT AT85110012T patent/ATE54468T1/de not_active IP Right Cessation
- 1985-08-08 CA CA000488281A patent/CA1251399A/en not_active Expired
- 1985-08-09 KR KR1019850005760A patent/KR920009733B1/ko not_active Expired
- 1985-08-09 US US06/764,128 patent/US4724210A/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009042074A (ja) * | 2007-08-09 | 2009-02-26 | Denka Seiken Co Ltd | 吸着担体及び吸着担体の製造方法 |
| EP2266675A2 (en) | 2009-06-25 | 2010-12-29 | Chisso Corporation | Chromatography medium, preparation method of the same, and method for producing virus vaccine using the chromatography medium |
| US9283542B2 (en) | 2009-06-25 | 2016-03-15 | Jnc Corporation | Chromatography medium, preparation method of the same, and method for producing virus vaccine using the chromatography medium |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0171086A3 (en) | 1988-01-13 |
| US4724210A (en) | 1988-02-09 |
| ATE54468T1 (de) | 1990-07-15 |
| KR920009733B1 (ko) | 1992-10-22 |
| CA1251399A (en) | 1989-03-21 |
| EP0171086B1 (en) | 1990-07-11 |
| EP0171086A2 (en) | 1986-02-12 |
| DE3578613D1 (de) | 1990-08-16 |
| KR870001835A (ko) | 1987-03-28 |
| JPS6230752B2 (enExample) | 1987-07-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |