JPS61162181A - 耐熱性キシラナ−ゼの製造法 - Google Patents
耐熱性キシラナ−ゼの製造法Info
- Publication number
- JPS61162181A JPS61162181A JP60003490A JP349085A JPS61162181A JP S61162181 A JPS61162181 A JP S61162181A JP 60003490 A JP60003490 A JP 60003490A JP 349085 A JP349085 A JP 349085A JP S61162181 A JPS61162181 A JP S61162181A
- Authority
- JP
- Japan
- Prior art keywords
- xylanase
- thermostable xylanase
- xylan
- heat
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 title claims abstract description 38
- 238000004519 manufacturing process Methods 0.000 title claims description 4
- 241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 abstract description 18
- 102000004190 Enzymes Human genes 0.000 abstract description 18
- 238000000034 method Methods 0.000 abstract description 11
- 229920001221 xylan Polymers 0.000 abstract description 10
- 150000004823 xylans Chemical class 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 8
- 239000001913 cellulose Substances 0.000 abstract description 6
- 229920002678 cellulose Polymers 0.000 abstract description 6
- 238000010438 heat treatment Methods 0.000 abstract description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract description 4
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 4
- 239000006228 supernatant Substances 0.000 abstract description 4
- 229910052799 carbon Inorganic materials 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 241000609240 Ambelania acida Species 0.000 abstract description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 2
- 239000010905 bagasse Substances 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract description 2
- 239000000843 powder Substances 0.000 abstract description 2
- 238000001556 precipitation Methods 0.000 abstract description 2
- 150000003839 salts Chemical class 0.000 abstract description 2
- 229910002651 NO3 Inorganic materials 0.000 abstract 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 abstract 1
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 22
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 15
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 15
- 229940088598 enzyme Drugs 0.000 description 15
- 239000000243 solution Substances 0.000 description 10
- 230000009471 action Effects 0.000 description 9
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 239000002028 Biomass Substances 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 4
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 108010059892 Cellulase Proteins 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 239000012225 czapek media Substances 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 150000002823 nitrates Chemical class 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- LGQKSQQRKHFMLI-SJYYZXOBSA-N (2s,3r,4s,5r)-2-[(3r,4r,5r,6r)-4,5,6-trihydroxyoxan-3-yl]oxyoxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)OC1 LGQKSQQRKHFMLI-SJYYZXOBSA-N 0.000 description 1
- GHCZTIFQWKKGSB-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;phosphoric acid Chemical compound OP(O)(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O GHCZTIFQWKKGSB-UHFFFAOYSA-N 0.000 description 1
- LGQKSQQRKHFMLI-UHFFFAOYSA-N 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(O)OC1 LGQKSQQRKHFMLI-UHFFFAOYSA-N 0.000 description 1
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- SQNRKWHRVIAKLP-UHFFFAOYSA-N D-xylobiose Natural products O=CC(O)C(O)C(CO)OC1OCC(O)C(O)C1O SQNRKWHRVIAKLP-UHFFFAOYSA-N 0.000 description 1
- 108010001817 Endo-1,4-beta Xylanases Proteins 0.000 description 1
- 101710101929 Endo-1,4-beta-xylanase 3 Proteins 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000533293 Sesbania emerus Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 241000223258 Thermomyces lanuginosus Species 0.000 description 1
- 229920002000 Xyloglucan Polymers 0.000 description 1
- UGXQOOQUZRUVSS-ZZXKWVIFSA-N [5-[3,5-dihydroxy-2-(1,3,4-trihydroxy-5-oxopentan-2-yl)oxyoxan-4-yl]oxy-3,4-dihydroxyoxolan-2-yl]methyl (e)-3-(4-hydroxyphenyl)prop-2-enoate Chemical compound OC1C(OC(CO)C(O)C(O)C=O)OCC(O)C1OC1C(O)C(O)C(COC(=O)\C=C\C=2C=CC(O)=CC=2)O1 UGXQOOQUZRUVSS-ZZXKWVIFSA-N 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 229920000617 arabinoxylan Polymers 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 108010047754 beta-Glucosidase Proteins 0.000 description 1
- 102000006995 beta-Glucosidase Human genes 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 229910001431 copper ion Inorganic materials 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- -1 mercury ions Chemical class 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 238000004816 paper chromatography Methods 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60003490A JPS61162181A (ja) | 1985-01-11 | 1985-01-11 | 耐熱性キシラナ−ゼの製造法 |
| US06/720,416 US4742005A (en) | 1985-01-07 | 1985-04-05 | Method for production of cellulolytic enzymes and method for saccharification of cellulosic materials therewith |
| EP85302505A EP0188050B1 (en) | 1985-01-07 | 1985-04-10 | Method for production of cellulolytic enzymes |
| DE8585302505T DE3583603D1 (de) | 1985-01-07 | 1985-04-10 | Verfahren zur produktion von zellulolytischen enzymen. |
| DK166685A DK164070C (da) | 1985-01-07 | 1985-04-12 | Fremgangsmaade til fremstilling af et sammensat cellulase/xylanase-enzympraeparat |
| US07/011,043 US4956291A (en) | 1985-01-07 | 1987-02-05 | Method for production of cellulolytic enzymes and method for saccharification of cellulosic materials therewith |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60003490A JPS61162181A (ja) | 1985-01-11 | 1985-01-11 | 耐熱性キシラナ−ゼの製造法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61162181A true JPS61162181A (ja) | 1986-07-22 |
| JPH0121957B2 JPH0121957B2 (enrdf_load_stackoverflow) | 1989-04-24 |
Family
ID=11558776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60003490A Granted JPS61162181A (ja) | 1985-01-07 | 1985-01-11 | 耐熱性キシラナ−ゼの製造法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61162181A (enrdf_load_stackoverflow) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01171484A (ja) * | 1987-12-25 | 1989-07-06 | Agency Of Ind Science & Technol | キシロオリゴシル転移酵素の製造法 |
| JPH01171485A (ja) * | 1987-12-25 | 1989-07-06 | Agency Of Ind Science & Technol | 新規なキシロオリゴシル転移酵素 |
| JPH01171492A (ja) * | 1987-12-25 | 1989-07-06 | Agency Of Ind Science & Technol | キシロオリゴ糖誘導体の製造方法 |
| EP0913467A4 (en) * | 1997-03-04 | 2003-01-02 | Meiji Seika Kaisha | MESOPHILE XYLANASES |
| JPWO2014061763A1 (ja) * | 2012-10-19 | 2016-09-05 | 国立研究開発法人産業技術総合研究所 | 新規キシラナーゼ |
-
1985
- 1985-01-11 JP JP60003490A patent/JPS61162181A/ja active Granted
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01171484A (ja) * | 1987-12-25 | 1989-07-06 | Agency Of Ind Science & Technol | キシロオリゴシル転移酵素の製造法 |
| JPH01171485A (ja) * | 1987-12-25 | 1989-07-06 | Agency Of Ind Science & Technol | 新規なキシロオリゴシル転移酵素 |
| JPH01171492A (ja) * | 1987-12-25 | 1989-07-06 | Agency Of Ind Science & Technol | キシロオリゴ糖誘導体の製造方法 |
| EP0913467A4 (en) * | 1997-03-04 | 2003-01-02 | Meiji Seika Kaisha | MESOPHILE XYLANASES |
| JPWO2014061763A1 (ja) * | 2012-10-19 | 2016-09-05 | 国立研究開発法人産業技術総合研究所 | 新規キシラナーゼ |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0121957B2 (enrdf_load_stackoverflow) | 1989-04-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK164070B (da) | Fremgangsmaade til fremstilling af et sammensat cellulase/xylanase-enzympraeparat | |
| EP0180952A2 (en) | Process for liquefying starch | |
| EP0184019A1 (en) | Thermostable alpha-amylase-producing, thermophilic anaerobic bacteria, thermostable alpha-amylase and process for producing the same | |
| JPH10501694A (ja) | ピロジクチウム・キシラナーゼ、アミラーゼ及びプルラナーゼ | |
| JPS61162181A (ja) | 耐熱性キシラナ−ゼの製造法 | |
| CN112111407A (zh) | 一种生产碱性果胶裂解酶的集胞藻pcc6803藻株及其构建方法 | |
| JPS59166081A (ja) | セルラ−ゼの製造法 | |
| CN114657073B (zh) | 一株高产纤维二糖酶的橘青霉菌株及其应用 | |
| DE2717333A1 (de) | Hitze- und saeurebestaendiges alpha- amylaseenzym, verfahren zu seiner herstellung und seine verwendung | |
| JP4039494B2 (ja) | 中温性キシラナーゼ | |
| JPS5836392A (ja) | エタノ−ルの製造法 | |
| JPS5917983A (ja) | アミラ−ゼｇ３の製造法 | |
| JPS6374490A (ja) | 培養におけるセルラ−ゼ製剤の使用及びセルロ−ス生産微生物の使用 | |
| JPS61162177A (ja) | セルラ−ゼの生産法 | |
| JPS592272B2 (ja) | セルラ−ゼの製造法 | |
| JPS61162167A (ja) | 新規なアクレモニウム・セルロリテイカスｔｎ株 | |
| JPS61162179A (ja) | セルラ−ゼの生産改良方法 | |
| JPH0365149B2 (enrdf_load_stackoverflow) | ||
| JPS59166095A (ja) | セルロ−スの処理方法 | |
| JPH01171484A (ja) | キシロオリゴシル転移酵素の製造法 | |
| JPS61162178A (ja) | セルラ−ゼの生産改良法 | |
| JPH0248231B2 (ja) | Shinkikishiranaazeoyobisonoseizoho | |
| JPH01171485A (ja) | 新規なキシロオリゴシル転移酵素 | |
| JPS59166083A (ja) | セルラ−ゼの生産方法 | |
| JPS5937956B2 (ja) | セルロ−スの糖化法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |