JPS6112631A - 免疫グロブリンｇ分子のプロテインａへの結合方法 - Google Patents

免疫グロブリンｇ分子のプロテインａへの結合方法

Info

Publication number
JPS6112631A
JPS6112631A JP60135808A JP13580885A JPS6112631A JP S6112631 A JPS6112631 A JP S6112631A JP 60135808 A JP60135808 A JP 60135808A JP 13580885 A JP13580885 A JP 13580885A JP S6112631 A JPS6112631 A JP S6112631A
Authority
JP
Japan
Prior art keywords
protein
immunoglobulin
molar
buffer
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP60135808A
Other languages
English (en)
Japanese (ja)
Other versions
JPH0455440B2 (enExample
Inventor
ヘクター　ジョオレツ‐サリナス
ゲイリー　スティーブン　オット
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24499435&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JPS6112631(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Publication of JPS6112631A publication Critical patent/JPS6112631A/ja
Publication of JPH0455440B2 publication Critical patent/JPH0455440B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/828—Protein A
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
JP60135808A 1984-06-22 1985-06-21 免疫グロブリンｇ分子のプロテインａへの結合方法 Granted JPS6112631A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US623797 1984-06-22
US06/623,797 US4704366A (en) 1984-06-22 1984-06-22 Process for binding IgG to protein A

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP3109760A Division JPH04234899A (ja) 1984-06-22 1991-04-15 免疫グロブリンｇ分子の単離方法

Publications (2)

Publication Number Publication Date
JPS6112631A true JPS6112631A (ja) 1986-01-21
JPH0455440B2 JPH0455440B2 (enExample) 1992-09-03

Family

ID=24499435

Family Applications (2)

Application Number Title Priority Date Filing Date
JP60135808A Granted JPS6112631A (ja) 1984-06-22 1985-06-21 免疫グロブリンｇ分子のプロテインａへの結合方法
JP3109760A Granted JPH04234899A (ja) 1984-06-22 1991-04-15 免疫グロブリンｇ分子の単離方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP3109760A Granted JPH04234899A (ja) 1984-06-22 1991-04-15 免疫グロブリンｇ分子の単離方法

Country Status (4)

Country Link
US (1) US4704366A (enExample)
JP (2) JPS6112631A (enExample)
DE (1) DE3521679A1 (enExample)
GB (1) GB2160530B (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4783872B2 (ja) * 2009-11-25 2011-09-28 パナソニック株式会社 免疫測定方法

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5283323A (en) * 1985-08-07 1994-02-01 The United States Of America As Represented By The Department Of Health And Human Services Method of producing immune response
US4977247A (en) * 1986-02-14 1990-12-11 Genex Corporation Immobilized protein G variants and the use thereof
SE459662B (sv) * 1986-03-21 1989-07-24 Pharmacia Ab Hybrid-dna-molekyl som kodar foer ett protein med samma igg specificitet som protein g, plasmid innehaallande densamma samt foerfarande foer framstaellning av proteinet
US5089605A (en) * 1987-03-13 1992-02-18 Repligen Corporation Immobilized immunoglobulin-binding proteins
JPS6419024A (en) * 1987-07-15 1989-01-23 Tosoh Corp Separation of immunoglobulin g subclass
US4981961A (en) * 1988-09-12 1991-01-01 Bioprobe International, Inc. Synthetic affinity ligand compositions and methods for purification and recovery of organic molecules
US4933435A (en) 1989-04-05 1990-06-12 Bioprobe International Antibody purification process
US5151504A (en) * 1989-11-17 1992-09-29 E. R. Squibb & Sons, Inc. Method for purification of monoclonal antibodies
US5077391A (en) * 1989-12-01 1991-12-31 Raison Robert L Purification of immunoglobulin M
JPH03185000A (ja) * 1989-12-15 1991-08-12 Tosoh Corp プロテインＡと免疫グロブリンＧ（ＩｇＧ）との結合方法
US5200471A (en) * 1990-11-05 1993-04-06 Minnesota Mining And Manufacturing Company Biomolecules covalently immobilized with a high bound specific biological activity and method of preparing same
US5245016A (en) * 1991-01-31 1993-09-14 University Of Utah Research Foundation Pseudomonas maltophilia immunoglobulin binding protein and methods for its use
WO1994022918A1 (en) * 1993-03-29 1994-10-13 Minnesota Mining And Manufacturing Company Method of coupling ligands within porous supports (p.e. azlactone) and uses thereof
US5593822A (en) * 1993-12-07 1997-01-14 John Wayne Cancer Institute IgG depleted serum preparations and methods for antibody production
US5561045A (en) * 1994-01-04 1996-10-01 Intracel Corporation Detection reagent, article, and immunoassay method
JPH08511280A (ja) * 1994-04-01 1996-11-26 ユニシン・テクノロジーズ・インコーポレーテッド 合成親和性リガンドを用いた免疫グロブリンの精製のための高塩濃度結合バッファー
US7276589B2 (en) * 2002-11-26 2007-10-02 Pdl Biopharma, Inc. Chimeric and humanized antibodies to α5β1 integrin that modulate angiogenesis
US7285268B2 (en) * 2002-11-26 2007-10-23 Pdl Biopharma, Inc. Chimeric and humanized antibodies to α5β1 integrin that modulate angiogenesis
CN100369930C (zh) * 2002-11-26 2008-02-20 Pdl生物制药股份有限公司 调节血管生成的α5β1整合素的嵌合的和人源化的抗体
NZ549893A (en) * 2004-03-24 2010-05-28 Facet Biotech Corp Use of anti-alpha5beta1 antibodiesin a stabilising solution to inhibit cancer cell proliferation
CA2694644A1 (en) * 2007-07-27 2009-02-05 Vanitha Ramakrishnan Pharmaceutical combinations
US20100158893A1 (en) * 2008-12-19 2010-06-24 Baxter International Inc. Systems and methods for obtaining immunoglobulin from blood
SG10201408384PA (en) 2009-12-18 2015-01-29 Novartis Ag Wash solution and method for affinity chromatography
US9062106B2 (en) 2011-04-27 2015-06-23 Abbvie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
FI3489685T3 (fi) 2011-11-21 2023-12-21 Zoetis Services Llc Signaalin vahvistaminen lateraalivirtaus- ja vastaavissa immunomäärityksissä
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
TW201348246A (zh) * 2012-05-21 2013-12-01 Abbvie Inc 利用蛋白質ａ親和性層析之人類、人類化或嵌合抗體之新穎純化
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
BR112015004467A2 (pt) 2012-09-02 2017-03-21 Abbvie Inc método para controlar a heterogeneidade de proteínas
AU2013381687A1 (en) 2013-03-12 2015-09-24 Abbvie Inc. Human antibodies that bind human TNF-alpha and methods of preparing the same
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
WO2015051293A2 (en) 2013-10-04 2015-04-09 Abbvie, Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
TWI691716B (zh) 2014-08-13 2020-04-21 美商艾巴希斯公司 電漿特異性結合搭配物檢定中之信號放大
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
DK3331818T3 (da) 2015-08-04 2024-08-12 Zoetis Services Llc Signalforstærkning i opløsningsbaserede plasmoniske assays med specifkke bindingspartnere
JP7106187B2 (ja) 2016-05-11 2022-07-26 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックスを保存する方法
CN109071613A (zh) 2016-05-11 2018-12-21 通用电气医疗集团生物工艺研发股份公司 分离基质
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
WO2017194593A1 (en) 2016-05-11 2017-11-16 Ge Healthcare Bioprocess R&D Ab Method of cleaning and/or sanitizing a separation matrix
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
IL268238B (en) 2017-01-30 2022-09-01 Abaxis Llc Tests for a specific plasmon-based solution for co-linking and metallic nanostructures
CN111902720B (zh) 2018-03-21 2025-02-07 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE413986B (sv) * 1973-03-23 1980-07-07 Exploaterings Ab Tbf Sett att separera amfipatiska emnen innehallande bade hydrofila och hydrofoba grupper samt gelprodukt for genomforande av separationen
US4430318A (en) * 1978-11-29 1984-02-07 The United States Of America As Represented By The Department Of Health And Human Services Immunoassay utilizing 125 I Protein A
US4230685A (en) * 1979-02-28 1980-10-28 Northwestern University Method of magnetic separation of cells and the like, and microspheres for use therein
AU570911B2 (en) * 1981-11-06 1988-03-31 Baylor College Of Medicine Immunoadsorbent based on immobilized protein a and its use

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4783872B2 (ja) * 2009-11-25 2011-09-28 パナソニック株式会社 免疫測定方法

Also Published As

Publication number Publication date
JPH0543719B2 (enExample) 1993-07-02
DE3521679A1 (de) 1986-01-02
GB2160530A (en) 1985-12-24
DE3521679C2 (enExample) 1989-08-24
US4704366A (en) 1987-11-03
JPH04234899A (ja) 1992-08-24
JPH0455440B2 (enExample) 1992-09-03
GB2160530B (en) 1987-10-28
GB8515261D0 (en) 1985-07-17

Similar Documents

Publication Publication Date Title
JPS6112631A (ja) 免疫グロブリンｇ分子のプロテインａへの結合方法
US11590486B2 (en) Solid phase for mixed-mode chromatographic purification of proteins
JP4776615B2 (ja) 抗体精製
JP5999899B2 (ja) 抗体のクロマトグラフィー精製法
HU217850B (hu) Eljárás antitest tisztítására
JPH02800A (ja) モノクローナル抗体の精製方法
Lihme et al. Thiophilic adsorption chromatography: the separation of serum proteins
JPWO1987001597A1 (ja) β↓２−ミクログロブリン除去カラム
Lihme et al. Divinylsulphone-activated agarose: formation of stable and non-leaking affinity matrices by immobilization of immunoglobulins and other proteins
Knudsen et al. Sulfone-aromatic ligands for thiophilic adsorption chromatography: purification of human and mouse immunoglobulins
JPH06107700A (ja) Ｆ ＶＩＩＩ／ｖＷＦ複合体吸着のための免疫吸着材
Sulk et al. Application of phase partitioning and thiophilic adsorption chromatography to the purification of monoclonal antibodies from cell culture fluid
Jean-Claude et al. The isolation and characterization of immunoglobulins, antibodies, and their constituent polypeptide chains
EP0449879A1 (en) Heterobifunctional antibodies having specificity for platelets and thrombolytic agents
US4841024A (en) Purification of antibodies
JPH0696598B2 (ja) 抗体の精製法
Juronen et al. FPLC purification of mouse monoclonal antibodies from ascitic fluid using blue DEAE and thiophilic sorbents
CA1233747A (en) Process for binding igg to protein a
CN111683976A (zh) 具有阴离子交换-疏水混合模式配体的色谱树脂
JP2961986B2 (ja) ポリクローナル抗体の分離精製方法
Leickt et al. Affinity screening for weak monoclonal antibodies
EP0432629A1 (en) Method for binding immunoglobulin G to protein A
JPH07146280A (ja) アフィニティクロマトグラフィー用吸着体及びこれを 用いた抗体の分離方法、抗体濃度の測定方法、抗体断 片の精製濃縮方法
JPH07270414A (ja) 高感度酵素免疫測定法
JPH02180899A (ja) 免疫グロブリンの精製方法