JPS60155129A - 固定化抗原または抗体の製造方法 - Google Patents
固定化抗原または抗体の製造方法Info
- Publication number
- JPS60155129A JPS60155129A JP1142684A JP1142684A JPS60155129A JP S60155129 A JPS60155129 A JP S60155129A JP 1142684 A JP1142684 A JP 1142684A JP 1142684 A JP1142684 A JP 1142684A JP S60155129 A JPS60155129 A JP S60155129A
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- film
- fibroin
- antigen
- immobilized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000427 antigen Substances 0.000 title claims abstract description 47
- 108091007433 antigens Proteins 0.000 title claims abstract description 47
- 102000036639 antigens Human genes 0.000 title claims abstract description 47
- 238000002360 preparation method Methods 0.000 title description 2
- 108010022355 Fibroins Proteins 0.000 claims abstract description 86
- 239000007864 aqueous solution Substances 0.000 claims abstract description 26
- 239000000758 substrate Substances 0.000 claims abstract description 16
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 abstract description 28
- 238000001179 sorption measurement Methods 0.000 abstract description 14
- 238000005259 measurement Methods 0.000 abstract description 10
- 238000005406 washing Methods 0.000 abstract description 8
- 239000000463 material Substances 0.000 abstract description 6
- 238000003127 radioimmunoassay Methods 0.000 abstract description 4
- 239000004809 Teflon Substances 0.000 abstract description 3
- 229920006362 Teflon® Polymers 0.000 abstract description 3
- 238000005266 casting Methods 0.000 abstract description 3
- 238000003018 immunoassay Methods 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 description 44
- 239000000243 solution Substances 0.000 description 36
- 239000002504 physiological saline solution Substances 0.000 description 21
- 238000006243 chemical reaction Methods 0.000 description 11
- 102100023635 Alpha-fetoprotein Human genes 0.000 description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000001035 drying Methods 0.000 description 9
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 8
- 229940084986 human chorionic gonadotropin Drugs 0.000 description 8
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 7
- 241000699666 Mus <mouse, genus> Species 0.000 description 7
- 102000013415 peroxidase activity proteins Human genes 0.000 description 6
- 108040007629 peroxidase activity proteins Proteins 0.000 description 6
- 238000003860 storage Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 5
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 5
- 229940098773 bovine serum albumin Drugs 0.000 description 5
- 238000007796 conventional method Methods 0.000 description 5
- 235000011187 glycerol Nutrition 0.000 description 5
- 229920000915 polyvinyl chloride Polymers 0.000 description 5
- 239000004800 polyvinyl chloride Substances 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 229940088597 hormone Drugs 0.000 description 4
- 239000005556 hormone Substances 0.000 description 4
- 238000005185 salting out Methods 0.000 description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
- 241000283707 Capra Species 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 3
- 235000011130 ammonium sulphate Nutrition 0.000 description 3
- 239000001110 calcium chloride Substances 0.000 description 3
- 229910001628 calcium chloride Inorganic materials 0.000 description 3
- 238000002425 crystallisation Methods 0.000 description 3
- 230000008025 crystallization Effects 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 108010088751 Albumins Proteins 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 2
- 108010013296 Sericins Proteins 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 description 2
- SMEGJBVQLJJKKX-HOTMZDKISA-N [(2R,3S,4S,5R,6R)-5-acetyloxy-3,4,6-trihydroxyoxan-2-yl]methyl acetate Chemical compound CC(=O)OC[C@@H]1[C@H]([C@@H]([C@H]([C@@H](O1)O)OC(=O)C)O)O SMEGJBVQLJJKKX-HOTMZDKISA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 229940081735 acetylcellulose Drugs 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 238000009739 binding Methods 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 229920002301 cellulose acetate Polymers 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000001112 coagulating effect Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000009849 deactivation Effects 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 125000000524 functional group Chemical group 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 238000003505 heat denaturation Methods 0.000 description 2
- GPGMRSSBVJNWRA-UHFFFAOYSA-N hydrochloride hydrofluoride Chemical compound F.Cl GPGMRSSBVJNWRA-UHFFFAOYSA-N 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 2
- YIXJRHPUWRPCBB-UHFFFAOYSA-N magnesium nitrate Chemical compound [Mg+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O YIXJRHPUWRPCBB-UHFFFAOYSA-N 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 239000012266 salt solution Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000009991 scouring Methods 0.000 description 2
- 239000000344 soap Substances 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 108700012359 toxins Proteins 0.000 description 2
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 1
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- DFGKGUXTPFWHIX-UHFFFAOYSA-N 6-[2-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]acetyl]-3H-1,3-benzoxazol-2-one Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)CC(=O)C1=CC2=C(NC(O2)=O)C=C1 DFGKGUXTPFWHIX-UHFFFAOYSA-N 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000218645 Cedrus Species 0.000 description 1
- 102000009016 Cholera Toxin Human genes 0.000 description 1
- 108010049048 Cholera Toxin Proteins 0.000 description 1
- 229910002483 Cu Ka Inorganic materials 0.000 description 1
- 229920001651 Cyanoacrylate Polymers 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 102000014702 Haptoglobin Human genes 0.000 description 1
- 108050005077 Haptoglobin Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000848653 Homo sapiens Tripartite motif-containing protein 26 Proteins 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- MWCLLHOVUTZFKS-UHFFFAOYSA-N Methyl cyanoacrylate Chemical compound COC(=O)C(=C)C#N MWCLLHOVUTZFKS-UHFFFAOYSA-N 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 102000004576 Placental Lactogen Human genes 0.000 description 1
- 108010003044 Placental Lactogen Proteins 0.000 description 1
- 239000000381 Placental Lactogen Substances 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 241000710799 Rubella virus Species 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- XCRMJTJUAIHPBT-UHFFFAOYSA-J [OH-].[OH-].[OH-].[OH-].[Cu+2].[Cu+2] Chemical compound [OH-].[OH-].[OH-].[OH-].[Cu+2].[Cu+2] XCRMJTJUAIHPBT-UHFFFAOYSA-J 0.000 description 1
- 108010026331 alpha-Fetoproteins Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003125 aqueous solvent Substances 0.000 description 1
- HNYOPLTXPVRDBG-UHFFFAOYSA-N barbituric acid Chemical compound O=C1CC(=O)NC(=O)N1 HNYOPLTXPVRDBG-UHFFFAOYSA-N 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000006208 butylation Effects 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 238000003163 cell fusion method Methods 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- QKSIFUGZHOUETI-UHFFFAOYSA-N copper;azane Chemical compound N.N.N.N.[Cu+2] QKSIFUGZHOUETI-UHFFFAOYSA-N 0.000 description 1
- 230000037029 cross reaction Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- CBMPTFJVXNIWHP-UHFFFAOYSA-L disodium;hydrogen phosphate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].OP([O-])([O-])=O.OC(=O)CC(O)(C(O)=O)CC(O)=O CBMPTFJVXNIWHP-UHFFFAOYSA-L 0.000 description 1
- 229960005309 estradiol Drugs 0.000 description 1
- 229930182833 estradiol Natural products 0.000 description 1
- -1 etc. Chemical compound 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- 102000046101 human AFP Human genes 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000000186 progesterone Substances 0.000 description 1
- 229960003387 progesterone Drugs 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000007788 roughening Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000002522 swelling effect Effects 0.000 description 1
- 229960003604 testosterone Drugs 0.000 description 1
- LYKRHFJZRFPQMB-UHFFFAOYSA-J tetrasodium dihydrogen phosphate 2-hydroxypropane-1,2,3-tricarboxylate Chemical compound [Na+].[Na+].[Na+].[Na+].OP(O)([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O LYKRHFJZRFPQMB-UHFFFAOYSA-J 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1142684A JPS60155129A (ja) | 1984-01-24 | 1984-01-24 | 固定化抗原または抗体の製造方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1142684A JPS60155129A (ja) | 1984-01-24 | 1984-01-24 | 固定化抗原または抗体の製造方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60155129A true JPS60155129A (ja) | 1985-08-15 |
| JPH0439623B2 JPH0439623B2 (enrdf_load_stackoverflow) | 1992-06-30 |
Family
ID=11777737
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1142684A Granted JPS60155129A (ja) | 1984-01-24 | 1984-01-24 | 固定化抗原または抗体の製造方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60155129A (enrdf_load_stackoverflow) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1987002780A1 (en) * | 1985-11-05 | 1987-05-07 | Kanebo, Ltd. | Method of immunoassay |
| WO2008127402A3 (en) * | 2006-11-03 | 2009-02-05 | Tufts College | Biopolymer sensor and method of manufacturing the same |
| US8574461B2 (en) | 2006-11-03 | 2013-11-05 | Tufts University | Electroactive biopolymer optical and electro-optical devices and method of manufacturing the same |
| US8747886B2 (en) | 2009-02-12 | 2014-06-10 | Tufts University | Nanoimprinting of silk fibroin structures for biomedical and biophotonic applications |
| US9016875B2 (en) | 2009-07-20 | 2015-04-28 | Tufts University/Trustees Of Tufts College | All-protein implantable, resorbable reflectors |
| US9142787B2 (en) | 2009-08-31 | 2015-09-22 | Tufts University | Silk transistor devices |
| US9513405B2 (en) | 2006-11-03 | 2016-12-06 | Tufts University | Biopolymer photonic crystals and method of manufacturing the same |
| US9599891B2 (en) | 2007-11-05 | 2017-03-21 | Trustees Of Tufts College | Fabrication of silk fibroin photonic structures by nanocontact imprinting |
| US9969134B2 (en) | 2006-11-03 | 2018-05-15 | Trustees Of Tufts College | Nanopatterned biopolymer optical device and method of manufacturing the same |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5425004B2 (ja) | 2010-07-05 | 2014-02-26 | 本田技研工業株式会社 | 鞍乗型車両の発光ダイオード点灯回路 |
-
1984
- 1984-01-24 JP JP1142684A patent/JPS60155129A/ja active Granted
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62106369A (ja) * | 1985-11-05 | 1987-05-16 | Kanebo Ltd | 免疫測定法 |
| WO1987002780A1 (en) * | 1985-11-05 | 1987-05-07 | Kanebo, Ltd. | Method of immunoassay |
| US9802374B2 (en) | 2006-11-03 | 2017-10-31 | Tufts University | Biopolymer sensor and method of manufacturing the same |
| WO2008127402A3 (en) * | 2006-11-03 | 2009-02-05 | Tufts College | Biopolymer sensor and method of manufacturing the same |
| US8529835B2 (en) | 2006-11-03 | 2013-09-10 | Tufts University | Biopolymer sensor and method of manufacturing the same |
| US8574461B2 (en) | 2006-11-03 | 2013-11-05 | Tufts University | Electroactive biopolymer optical and electro-optical devices and method of manufacturing the same |
| US10280204B2 (en) | 2006-11-03 | 2019-05-07 | Tufts University | Electroactive biopolymer optical and electro-optical devices and method of manufacturing the same |
| US10040834B2 (en) | 2006-11-03 | 2018-08-07 | Tufts University | Biopolymer optofluidic device and method of manufacturing the same |
| US9969134B2 (en) | 2006-11-03 | 2018-05-15 | Trustees Of Tufts College | Nanopatterned biopolymer optical device and method of manufacturing the same |
| US9513405B2 (en) | 2006-11-03 | 2016-12-06 | Tufts University | Biopolymer photonic crystals and method of manufacturing the same |
| US9599891B2 (en) | 2007-11-05 | 2017-03-21 | Trustees Of Tufts College | Fabrication of silk fibroin photonic structures by nanocontact imprinting |
| US9603810B2 (en) | 2009-02-12 | 2017-03-28 | Tufts University | Nanoimprinting of silk fibroin structures for biomedical and biophotonic applications |
| US8747886B2 (en) | 2009-02-12 | 2014-06-10 | Tufts University | Nanoimprinting of silk fibroin structures for biomedical and biophotonic applications |
| US9016875B2 (en) | 2009-07-20 | 2015-04-28 | Tufts University/Trustees Of Tufts College | All-protein implantable, resorbable reflectors |
| US9142787B2 (en) | 2009-08-31 | 2015-09-22 | Tufts University | Silk transistor devices |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0439623B2 (enrdf_load_stackoverflow) | 1992-06-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4478946A (en) | Carrier bound immunosorbent | |
| JPH0322944B2 (enrdf_load_stackoverflow) | ||
| JPH07117536B2 (ja) | 体液中の特異的に結合可能の物質の検出方法及びリガンド受容体 | |
| JPH07506195A (ja) | 特異的結合性アッセイ試薬の固定化 | |
| JPH06222058A (ja) | 三座共役体、その製造方法及びその使用方法 | |
| EP0269092A2 (de) | Verfahren zur Bestimmung einer spezifisch bindefähigen Substanz | |
| JPS5924388B2 (ja) | 免疫化学および酵素的試験のための容器 | |
| JPS60155129A (ja) | 固定化抗原または抗体の製造方法 | |
| JPH0543600A (ja) | 抗体または抗原固定化絹フイブロイン膜および免疫測定用センサー | |
| US4975532A (en) | Method to derivatize dextran | |
| JPH0340830B2 (enrdf_load_stackoverflow) | ||
| JPH08503066A (ja) | 固相への化学的結合方法 | |
| JPS61151463A (ja) | 免疫反応性多孔性キヤリア材料の製法 | |
| EP0609144A1 (fr) | Dosage immunométrique d'un antigène ou d'un haptène | |
| JPH0579943B2 (enrdf_load_stackoverflow) | ||
| JPH05249113A (ja) | 分離・分析方法及び装置 | |
| JPS61130263A (ja) | 二官能性ハプテンおよび免疫化学的ハプテン測定法 | |
| JPH01209370A (ja) | 免疫活性物質の測定法及び測定試薬 | |
| JPH02500687A (ja) | 固相マトリツクスの製法 | |
| JPS62106369A (ja) | 免疫測定法 | |
| JP2003523503A (ja) | 疎水性固相に親和性試薬を固定する方法 | |
| JPS61241665A (ja) | 安定化された固相試薬 | |
| JPS607362A (ja) | 酵素を用いた抗原決定基具有物質の測定法 | |
| JPS6234059A (ja) | 固相化試薬の安定化剤 | |
| JPS628054A (ja) | 固定化抗原 |