JPS58149677A - L−グルタミン酸オキシダ−ゼ(h↓2o↓2・ジエネレイテイング)およびその製造法、ならびに分析方法 - Google Patents
L−グルタミン酸オキシダ−ゼ(h↓2o↓2・ジエネレイテイング)およびその製造法、ならびに分析方法Info
- Publication number
- JPS58149677A JPS58149677A JP57033497A JP3349782A JPS58149677A JP S58149677 A JPS58149677 A JP S58149677A JP 57033497 A JP57033497 A JP 57033497A JP 3349782 A JP3349782 A JP 3349782A JP S58149677 A JPS58149677 A JP S58149677A
- Authority
- JP
- Japan
- Prior art keywords
- glutamic acid
- mol
- acid
- enzyme
- generating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- 229960002989 glutamic acid Drugs 0.000 title claims abstract description 86
- 102000004316 Oxidoreductases Human genes 0.000 title claims abstract description 20
- 108090000854 Oxidoreductases Proteins 0.000 title claims abstract description 20
- 238000004458 analytical method Methods 0.000 title claims abstract description 13
- 102000004190 Enzymes Human genes 0.000 claims abstract description 74
- 108090000790 Enzymes Proteins 0.000 claims abstract description 74
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 64
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 claims abstract description 54
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims abstract description 48
- 229910021529 ammonia Inorganic materials 0.000 claims abstract description 24
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000001301 oxygen Substances 0.000 claims abstract description 24
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 24
- HWXBTNAVRSUOJR-UHFFFAOYSA-N alpha-hydroxyglutaric acid Natural products OC(=O)C(O)CCC(O)=O HWXBTNAVRSUOJR-UHFFFAOYSA-N 0.000 claims abstract description 21
- 229940009533 alpha-ketoglutaric acid Drugs 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 21
- 241000187747 Streptomyces Species 0.000 claims abstract description 20
- 108010069325 L-glutamate oxidase Proteins 0.000 claims description 54
- 230000000694 effects Effects 0.000 claims description 50
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- 239000012085 test solution Substances 0.000 claims description 14
- 238000005259 measurement Methods 0.000 claims description 13
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- 239000004220 glutamic acid Substances 0.000 claims description 11
- 238000004519 manufacturing process Methods 0.000 claims description 10
- 238000012360 testing method Methods 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 8
- 229940049906 glutamate Drugs 0.000 claims description 8
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 6
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- 229930195712 glutamate Natural products 0.000 claims description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 2
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- 101710098398 Probable alanine aminotransferase, mitochondrial Proteins 0.000 claims 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 1
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- 241000187180 Streptomyces sp. Species 0.000 abstract 1
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 22
- 239000000872 buffer Substances 0.000 description 21
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- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 6
- 238000000691 measurement method Methods 0.000 description 6
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- GMKMEZVLHJARHF-SYDPRGILSA-N meso-2,6-diaminopimelic acid Chemical group [O-]C(=O)[C@@H]([NH3+])CCC[C@@H]([NH3+])C([O-])=O GMKMEZVLHJARHF-SYDPRGILSA-N 0.000 description 5
- 239000008107 starch Substances 0.000 description 5
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- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- CKLJMWTZIZZHCS-UWTATZPHSA-N L-Aspartic acid Natural products OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 description 4
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 4
- 102000003992 Peroxidases Human genes 0.000 description 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- 239000000370 acceptor Substances 0.000 description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 4
- 235000011130 ammonium sulphate Nutrition 0.000 description 4
- 150000001448 anilines Chemical class 0.000 description 4
- 229960005261 aspartic acid Drugs 0.000 description 4
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 4
- 229960003067 cystine Drugs 0.000 description 4
- 238000000502 dialysis Methods 0.000 description 4
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 4
- 239000007986 glycine-NaOH buffer Substances 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 108040007629 peroxidase activity proteins Proteins 0.000 description 4
- 150000002989 phenols Chemical class 0.000 description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- 238000011218 seed culture Methods 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 229960004441 tyrosine Drugs 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 3
- 108010008292 L-Amino Acid Oxidase Proteins 0.000 description 3
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 3
- 102000007070 L-amino-acid oxidase Human genes 0.000 description 3
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 3
- 239000004158 L-cystine Substances 0.000 description 3
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- 101710132602 Peroxidase 5 Proteins 0.000 description 3
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- 150000001413 amino acids Chemical class 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
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- 241000456624 Actinobacteria bacterium Species 0.000 description 2
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
- C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/886—Streptomyces
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP57033497A JPS58149677A (ja) | 1982-03-02 | 1982-03-02 | L−グルタミン酸オキシダ−ゼ(h↓2o↓2・ジエネレイテイング)およびその製造法、ならびに分析方法 |
DE19833307607 DE3307607A1 (de) | 1982-03-02 | 1983-03-01 | L-glutaminsaeure-oxidase, verfahren zu deren herstellung und verwendung fuer analysenmethode |
DE3348379A DE3348379C2 (en, 2012) | 1982-03-02 | 1983-03-01 | |
KR1019830000832A KR870000509B1 (ko) | 1982-03-02 | 1983-03-02 | L-글루탐산 산화효소(h₂o₂생성), 그 제법 및 분석방법 |
US06/472,174 US4605615A (en) | 1982-03-02 | 1983-03-02 | L-glutamic acid oxidase (H2 O2 -generating), its production and analytical method therefor |
IT19856/83A IT1161590B (it) | 1982-03-02 | 1983-03-02 | Acido-l-glutammico ossidasi (generatore di h202),sua produzione e saggio analitico che lo utilizza |
FR8303412A FR2522680B1 (fr) | 1982-03-02 | 1983-03-02 | Oxydase d'acide l-glutamique (productrice de h2o2), sa production et methode d'analyse l'utilisant |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP57033497A JPS58149677A (ja) | 1982-03-02 | 1982-03-02 | L−グルタミン酸オキシダ−ゼ(h↓2o↓2・ジエネレイテイング)およびその製造法、ならびに分析方法 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP1091676A Division JPH02163080A (ja) | 1989-04-11 | 1989-04-11 | L―グルタミン酸オキシダーゼ(h↓2o↓2・ジエネレイテイング)およびその製造法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS58149677A true JPS58149677A (ja) | 1983-09-06 |
JPH0329399B2 JPH0329399B2 (en, 2012) | 1991-04-24 |
Family
ID=12388181
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP57033497A Granted JPS58149677A (ja) | 1982-03-02 | 1982-03-02 | L−グルタミン酸オキシダ−ゼ(h↓2o↓2・ジエネレイテイング)およびその製造法、ならびに分析方法 |
Country Status (6)
Country | Link |
---|---|
US (1) | US4605615A (en, 2012) |
JP (1) | JPS58149677A (en, 2012) |
KR (1) | KR870000509B1 (en, 2012) |
DE (2) | DE3348379C2 (en, 2012) |
FR (1) | FR2522680B1 (en, 2012) |
IT (1) | IT1161590B (en, 2012) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5942896A (ja) * | 1982-08-21 | 1984-03-09 | Yamasa Shoyu Co Ltd | L−グルタミン酸の分析法、分析用試薬および分析用キット |
CN114657088A (zh) * | 2022-02-17 | 2022-06-24 | 大自然生物集团有限公司 | 用于催化生产α-酮戊二酸的专用高温菌及催化生产方法 |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU565635B2 (en) * | 1982-06-29 | 1987-09-24 | Yamasa Shoyu K.K. | L-glutamic acid oxidase |
JPS6041500A (ja) * | 1983-08-12 | 1985-03-05 | Yamasa Shoyu Co Ltd | アンモニアの定量法 |
DE3333453A1 (de) * | 1983-09-16 | 1985-04-11 | Hoechst Ag, 6230 Frankfurt | L-aminosaeure-oxidase aus hefen der gattung cryptococcus, ihre herstellung und verwendung |
US5464775A (en) * | 1991-09-16 | 1995-11-07 | Chimera Research And Chemical, Inc. | Method of detecting adulterant in urine |
RU2170252C2 (ru) * | 1999-03-11 | 2001-07-10 | Общество с ограниченной ответственностью "Импакт" | Штамм streptomyces sp. z-11-6-продуцент внеклеточной l-глутаматоксидазы |
ATE387501T1 (de) * | 2000-04-19 | 2008-03-15 | Yamasa Corp | L-glutamatoxidase |
US7297800B2 (en) * | 2001-12-27 | 2007-11-20 | Ajinomoto Co., Inc. | Process of producing glutamate derivatives |
CA2714881C (en) | 2002-08-26 | 2012-10-23 | Ajinomoto Co., Inc. | Novel aldolase and production process of substituted .alpha.-keto acids |
CA2507225C (en) | 2002-12-09 | 2012-02-07 | Ajinomoto Co., Inc. | Mutated d-aminotransferase and method for producing optically active glutamic acid derivatives using the same |
US7180370B2 (en) * | 2004-09-01 | 2007-02-20 | Micron Technology, Inc. | CMOS amplifiers with frequency compensating capacitors |
CA2716603A1 (en) * | 2007-03-05 | 2009-09-12 | Advanced Liquid Logic, Inc. | Hydrogen peroxide droplet-based assays |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5743685A (en) * | 1980-08-28 | 1982-03-11 | Banyu Pharmaceut Co Ltd | L-glutamic oxidase |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU565635B2 (en) * | 1982-06-29 | 1987-09-24 | Yamasa Shoyu K.K. | L-glutamic acid oxidase |
-
1982
- 1982-03-02 JP JP57033497A patent/JPS58149677A/ja active Granted
-
1983
- 1983-03-01 DE DE3348379A patent/DE3348379C2/de not_active Expired - Lifetime
- 1983-03-01 DE DE19833307607 patent/DE3307607A1/de active Granted
- 1983-03-02 KR KR1019830000832A patent/KR870000509B1/ko not_active Expired
- 1983-03-02 FR FR8303412A patent/FR2522680B1/fr not_active Expired
- 1983-03-02 US US06/472,174 patent/US4605615A/en not_active Expired - Lifetime
- 1983-03-02 IT IT19856/83A patent/IT1161590B/it active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5743685A (en) * | 1980-08-28 | 1982-03-11 | Banyu Pharmaceut Co Ltd | L-glutamic oxidase |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5942896A (ja) * | 1982-08-21 | 1984-03-09 | Yamasa Shoyu Co Ltd | L−グルタミン酸の分析法、分析用試薬および分析用キット |
CN114657088A (zh) * | 2022-02-17 | 2022-06-24 | 大自然生物集团有限公司 | 用于催化生产α-酮戊二酸的专用高温菌及催化生产方法 |
CN114657088B (zh) * | 2022-02-17 | 2023-10-13 | 大自然生物集团有限公司 | 用于催化生产α-酮戊二酸的专用高温菌及催化生产方法 |
Also Published As
Publication number | Publication date |
---|---|
DE3348379C2 (en, 2012) | 1992-12-17 |
JPH0329399B2 (en, 2012) | 1991-04-24 |
IT8319856A1 (it) | 1984-09-02 |
IT1161590B (it) | 1987-03-18 |
DE3307607C2 (en, 2012) | 1990-12-20 |
FR2522680B1 (fr) | 1986-06-27 |
KR840004161A (ko) | 1984-10-10 |
IT8319856A0 (it) | 1983-03-02 |
DE3307607A1 (de) | 1983-09-29 |
FR2522680A1 (fr) | 1983-09-09 |
KR870000509B1 (ko) | 1987-03-13 |
US4605615A (en) | 1986-08-12 |
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