JPS54135789A - Preparation of human insulin - Google Patents

Preparation of human insulin

Info

Publication number
JPS54135789A
JPS54135789A JP4357478A JP4357478A JPS54135789A JP S54135789 A JPS54135789 A JP S54135789A JP 4357478 A JP4357478 A JP 4357478A JP 4357478 A JP4357478 A JP 4357478A JP S54135789 A JPS54135789 A JP S54135789A
Authority
JP
Japan
Prior art keywords
insulin
pig
human insulin
alanine
protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP4357478A
Other languages
Japanese (ja)
Other versions
JPS637760B2 (en
Inventor
Hitoshi Kimura
Masami Soejima
Takeji Masaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nippon Soda Co Ltd
Original Assignee
Nippon Soda Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Soda Co Ltd filed Critical Nippon Soda Co Ltd
Priority to JP4357478A priority Critical patent/JPS54135789A/en
Publication of JPS54135789A publication Critical patent/JPS54135789A/en
Publication of JPS637760B2 publication Critical patent/JPS637760B2/ja
Granted legal-status Critical Current

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PURPOSE: To prepare human insulin easily, under a condition similar to that of the conventional process, by condensing a des-B30-alanine-pig-insulin obtained by converting pig insulin to BOC with protease I, with protected threonine.
CONSTITUTION: Pig insulin is converted to des-B30-alanine-pig insulin of formula I (R is an amino-protecting group which is eliminated by proton-solvolysis or β-elimination) or its pentaalkyl ester by reacting with protease I produced by Achromobacter lyticus. The compound I is condensed with an amino acid II in a solvent such as DMF, THF, etc., in the presence of triethylamine, etc., at pH7W 8, using 1-hydroxybenzotriazole and dicyclohexyl carbodiimide as condensing agent, and the protecting group is eliminated from the condensation product by treating with trifluoroacetic acid, to afford the objective human insulin.
COPYRIGHT: (C)1979,JPO&Japio
JP4357478A 1978-04-13 1978-04-13 Preparation of human insulin Granted JPS54135789A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP4357478A JPS54135789A (en) 1978-04-13 1978-04-13 Preparation of human insulin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4357478A JPS54135789A (en) 1978-04-13 1978-04-13 Preparation of human insulin

Publications (2)

Publication Number Publication Date
JPS54135789A true JPS54135789A (en) 1979-10-22
JPS637760B2 JPS637760B2 (en) 1988-02-18

Family

ID=12667511

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4357478A Granted JPS54135789A (en) 1978-04-13 1978-04-13 Preparation of human insulin

Country Status (1)

Country Link
JP (1) JPS54135789A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57155997A (en) * 1981-10-30 1982-09-27 Shionogi & Co Ltd Insulin derivative
JPS62116598A (en) * 1981-10-30 1987-05-28 Shionogi & Co Ltd Production of insulin derivative

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5141378A (en) * 1974-10-03 1976-04-07 Teijin Ltd

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5141378A (en) * 1974-10-03 1976-04-07 Teijin Ltd

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57155997A (en) * 1981-10-30 1982-09-27 Shionogi & Co Ltd Insulin derivative
JPS62116598A (en) * 1981-10-30 1987-05-28 Shionogi & Co Ltd Production of insulin derivative
JPS6312480B2 (en) * 1981-10-30 1988-03-18 Shionogi & Co
JPH0150719B2 (en) * 1981-10-30 1989-10-31 Shionogi & Co

Also Published As

Publication number Publication date
JPS637760B2 (en) 1988-02-18

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