JPH09512899A - 結合アッセイ - Google Patents
結合アッセイInfo
- Publication number
- JPH09512899A JPH09512899A JP7517854A JP51785495A JPH09512899A JP H09512899 A JPH09512899 A JP H09512899A JP 7517854 A JP7517854 A JP 7517854A JP 51785495 A JP51785495 A JP 51785495A JP H09512899 A JPH09512899 A JP H09512899A
- Authority
- JP
- Japan
- Prior art keywords
- binding
- analyte
- reagent
- binding site
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/973—Simultaneous determination of more than one analyte
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/80—Fluorescent dyes, e.g. rhodamine
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/808—Automated or kit
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.液体試料中の被分析物の濃度を測定する方法であって、 (a)液体試料を被分析物に特異的な結合部位を有する結合試薬と、結合部位 の小部分が被分析物によって占有されるように接触させ、 (b)第1および第2の現像試薬を用いて結合試薬を逆滴定し、ここで、第1 の現像試薬は占有されていない結合部位に結合でき、第1の標識を有するもので あり、第2の現像試薬は結合された被分析物または占有された結合部位に結合で き、第1の標識とは異なる第2の標識を有しているものであり、 (c)第1および第2の標識によって生じたシグナルを測定して、被分析物に よって占有された結合部位の部分の表わす数値を供級し、 (d)該数値を既知の濃度の被分析物を含有する一連の標準溶液から得られる 対応する数値と比較して、液体試料中の被分析物の濃度を得る: ことを含んで成る方法。 2.試料中の被分析物の周囲の濃度が著しく乱されないように少量の結合試薬 を用いる請求項1に記載の方法。 3.少量の結合試薬が0.1 V/Kモル以下であり、Vは液体試料の容積であっ て、Kは結合試薬に結合する被分析物についての会合常数である請求項2に記載 の方法。 4.各結合試薬が与えられた被分析物に対して特異的な結合部位を有している 多数の結合試薬を用いて、液体試料中の多数の異なった被分析物の濃度を測定す る上記請求項のいずれか1項に記載の方法。 5.結合試薬が支持体上の別々の位置に固定化されている上記請求項のいずれ か1項に記載の方法。 6.多数の結合試薬のためのアッセイにおいて、多数の異なった結合試薬を、 支持体上のそれらの位置によって別々に区別された異なった結合試薬を含有する 別々の位置を滴定するのに、同じ第1および第2の現像試薬を用いる請求項5に 記載の方法。 7.特定の被分析物に特異的な結合部位を有する結合試薬が、その被分析物の 一連の濃度測定値が得られるように、支持体上の多数の別々の位置に固定化され る上記の請求項のいずれか1項に記載の方法。 8.別々の位置がミクロスポットである請求項5から7のいずれか1項に記載 の方法。 9.ミクロスポットの面積が1mm2より小さい請求項8に記載の方法。 10.結合試薬が抗原に対する結合部位を有する抗体であるか、核酸被分析物に 結合し得るオリゴヌクレオチドである上記請求項のいずれか1項に記載の方法。 11.上記請求項のいずれか1項に記載の方法による液体試料中の被分析物の濃 度を測定するためのキットであって、 (a)被分析物に特異的な結合部位を有する結合試薬である結合試薬を付着さ せた固体支持体、 (b)第1の現像試薬は占有されていない結合部位に結合でき、 第1の標識を有するものであり、第2の現像試薬は結合された被分析物または 占有された結合部位に結合でき、第1の標識とは異なる第2の標識を有している ものである、第1および第2の現像試薬を含んでなる滴定試薬: を含んでなるキット。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9326451.3 | 1993-12-24 | ||
GB939326451A GB9326451D0 (en) | 1993-12-24 | 1993-12-24 | Binding assay |
PCT/GB1994/002813 WO1995018376A1 (en) | 1993-12-24 | 1994-12-23 | Back-titration assay using two different markers |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH09512899A true JPH09512899A (ja) | 1997-12-22 |
JP3424831B2 JP3424831B2 (ja) | 2003-07-07 |
Family
ID=10747225
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP51785495A Expired - Lifetime JP3424831B2 (ja) | 1993-12-24 | 1994-12-23 | 結合アッセイ |
Country Status (8)
Country | Link |
---|---|
US (1) | US5834319A (ja) |
EP (1) | EP0736175B1 (ja) |
JP (1) | JP3424831B2 (ja) |
AU (1) | AU1321795A (ja) |
DE (1) | DE69411189T2 (ja) |
ES (1) | ES2120169T3 (ja) |
GB (1) | GB9326451D0 (ja) |
WO (1) | WO1995018376A1 (ja) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6775621B1 (en) | 1998-12-15 | 2004-08-10 | Hitachi Software Engineering Co., Ltd. | Degree of hybridization detection method |
JP2020506395A (ja) * | 2017-02-09 | 2020-02-27 | プロメガ コーポレイションPromega Corporation | 検体検出イムノアッセイ |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE59607877D1 (de) * | 1996-03-13 | 2001-11-15 | Hoffmann La Roche | Optochemisches Verfahren und Messanordnung zur Messung der Konzentration eines Analyten |
US7157234B2 (en) | 1997-10-24 | 2007-01-02 | Beckman Coulter, Inc. | Detection of very low quantities of analyte bound to a solid phase |
EP2189791A3 (en) | 1998-02-04 | 2011-03-09 | Life Technologies Corporation | Microarrays and uses therefor |
DE10015448A1 (de) * | 2000-03-29 | 2001-10-11 | November Ag Molekulare Medizin | Verfahren zum Nachweisen und/oder Quantifizieren des Bindens erster Moleküle an dazu affine zweite Moleküle |
CN1654956A (zh) | 2000-05-04 | 2005-08-17 | 耶鲁大学 | 用于筛选蛋白活性的高密度蛋白阵列 |
GB0029729D0 (en) * | 2000-12-06 | 2001-01-17 | Ids Ltd | Method for detection of vitamin D metabolites |
EP1377827A2 (en) * | 2001-04-10 | 2004-01-07 | NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen in Reutlingen Stiftung Bürgerlichen Rechts | Method for determining analyte molecules in a sample |
US20040248323A1 (en) | 2003-06-09 | 2004-12-09 | Protometrix, Inc. | Methods for conducting assays for enzyme activity on protein microarrays |
GB0421838D0 (en) | 2004-09-30 | 2004-11-03 | Congenia S R L | Cancer markers |
WO2006132666A1 (en) * | 2005-06-06 | 2006-12-14 | Decision Biomarkers, Inc. | Assays based on liquid flow over arrays |
GB201016403D0 (en) | 2010-09-29 | 2010-11-10 | Bath Ventures | Novel interaction between staphylococcus aureus Sbi and C3d protiens |
US9075021B2 (en) | 2012-02-17 | 2015-07-07 | Toyota Motor Engineering & Manufacturing North America, Inc. | Methods and systems for monitoring content of coating solutions using automated titration devices |
CN108139395A (zh) * | 2015-10-16 | 2018-06-08 | 东洋纺株式会社 | 免疫层析试验片 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3380777D1 (en) * | 1982-08-27 | 1989-11-30 | Roger Philip Ekins | Measurement of analyte concentration |
WO1988001058A1 (en) * | 1986-08-06 | 1988-02-11 | Roger Philip Ekins | Determination of analyte concentration using two labelling markers |
US5098846A (en) * | 1987-05-27 | 1992-03-24 | Mclean Hospital | Solid phase protein assay by solid phase free site titration |
GB8803000D0 (en) * | 1988-02-10 | 1988-03-09 | Ekins Roger Philip | Determination of ambient concentrations of several analytes |
-
1993
- 1993-12-24 GB GB939326451A patent/GB9326451D0/en active Pending
-
1994
- 1994-12-23 DE DE69411189T patent/DE69411189T2/de not_active Expired - Lifetime
- 1994-12-23 ES ES95904615T patent/ES2120169T3/es not_active Expired - Lifetime
- 1994-12-23 WO PCT/GB1994/002813 patent/WO1995018376A1/en active IP Right Grant
- 1994-12-23 AU AU13217/95A patent/AU1321795A/en not_active Abandoned
- 1994-12-23 US US08/663,172 patent/US5834319A/en not_active Expired - Lifetime
- 1994-12-23 EP EP95904615A patent/EP0736175B1/en not_active Expired - Lifetime
- 1994-12-23 JP JP51785495A patent/JP3424831B2/ja not_active Expired - Lifetime
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6775621B1 (en) | 1998-12-15 | 2004-08-10 | Hitachi Software Engineering Co., Ltd. | Degree of hybridization detection method |
JP2020506395A (ja) * | 2017-02-09 | 2020-02-27 | プロメガ コーポレイションPromega Corporation | 検体検出イムノアッセイ |
Also Published As
Publication number | Publication date |
---|---|
JP3424831B2 (ja) | 2003-07-07 |
ES2120169T3 (es) | 1998-10-16 |
DE69411189D1 (de) | 1998-07-23 |
AU1321795A (en) | 1995-07-17 |
US5834319A (en) | 1998-11-10 |
WO1995018376A1 (en) | 1995-07-06 |
GB9326451D0 (en) | 1994-02-23 |
EP0736175B1 (en) | 1998-06-17 |
DE69411189T2 (de) | 1998-11-19 |
EP0736175A1 (en) | 1996-10-09 |
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