ES2120169T3 - Ensayo de retro-titulacion utilizando dos marcadores diferentes. - Google Patents

Ensayo de retro-titulacion utilizando dos marcadores diferentes.

Info

Publication number
ES2120169T3
ES2120169T3 ES95904615T ES95904615T ES2120169T3 ES 2120169 T3 ES2120169 T3 ES 2120169T3 ES 95904615 T ES95904615 T ES 95904615T ES 95904615 T ES95904615 T ES 95904615T ES 2120169 T3 ES2120169 T3 ES 2120169T3
Authority
ES
Spain
Prior art keywords
analyte
retro
union
different markers
agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95904615T
Other languages
English (en)
Inventor
Roger Philip Ekins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Multilyte Ltd
Original Assignee
Multilyte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=10747225&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2120169(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Multilyte Ltd filed Critical Multilyte Ltd
Application granted granted Critical
Publication of ES2120169T3 publication Critical patent/ES2120169T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/973—Simultaneous determination of more than one analyte
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A UN METODO Y EQUIPO PARA DETERMINAR LA CONCENTRACION DE UN ANALITO (6) EN UNA MUESTRA LIQUIDA, UTILIZANDO UN AGENTE AGLOMERANTE (29 CAPAZ DE AGLOMERAR EL ANALITO (6) Y LOS AGENTES DE DESARROLLO PRIMEROS Y SEGUNDOS (8,12), SIENDO CAPAZ EL PRIMERO (8) DE UNIRSE A LOCALIZACIONES DE UNION DESOCUPADAS DEL AGENTE DE UNION (2), Y TENIENDO UN PRIMER MARCADOR (10); EL SEGUNDO AGENTE DE DESARROLLO (12) ES CAPAZ DE UNIRSE AL ANALITO UNIDO O A LAS LOCALIZACIONES DE UNION OCUPADAS, Y TENIENDO UN SEGUNDO MARCADOR (14) DIFERENTE DEL PRIMERO. LA CONCENTRACION DEL ANALITO SE DETERMINA MIDIENDO LAS SEÑALES DE LOS MARCADORES EN LOS PRIMEROS Y SEGUNDOS AGENTES DE DESARROLLO (8,12). ASI, EL METODO COMBINA LOS METODOS COMPETITIVO Y NO COMPETITIVO DE LA TECNICA ANTERIOR, Y PREFERIBLEMENTE POSEE LA VENTAJA DE EXTENDER EL AMBITO DE TRABAJO Y/O SENSIBILIDAD DEL ENSAYO.
ES95904615T 1993-12-24 1994-12-23 Ensayo de retro-titulacion utilizando dos marcadores diferentes. Expired - Lifetime ES2120169T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB939326451A GB9326451D0 (en) 1993-12-24 1993-12-24 Binding assay

Publications (1)

Publication Number Publication Date
ES2120169T3 true ES2120169T3 (es) 1998-10-16

Family

ID=10747225

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95904615T Expired - Lifetime ES2120169T3 (es) 1993-12-24 1994-12-23 Ensayo de retro-titulacion utilizando dos marcadores diferentes.

Country Status (8)

Country Link
US (1) US5834319A (es)
EP (1) EP0736175B1 (es)
JP (1) JP3424831B2 (es)
AU (1) AU1321795A (es)
DE (1) DE69411189T2 (es)
ES (1) ES2120169T3 (es)
GB (1) GB9326451D0 (es)
WO (1) WO1995018376A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE59607877D1 (de) * 1996-03-13 2001-11-15 Hoffmann La Roche Optochemisches Verfahren und Messanordnung zur Messung der Konzentration eines Analyten
US7157234B2 (en) 1997-10-24 2007-01-02 Beckman Coulter, Inc. Detection of very low quantities of analyte bound to a solid phase
AU2583899A (en) 1998-02-04 1999-08-23 Invitrogen Corporation Microarrays and uses therefor
JP3442327B2 (ja) 1998-12-15 2003-09-02 日立ソフトウエアエンジニアリング株式会社 ハイブリダイゼーション検出方法及びバイオチップ
DE10015448A1 (de) * 2000-03-29 2001-10-11 November Ag Molekulare Medizin Verfahren zum Nachweisen und/oder Quantifizieren des Bindens erster Moleküle an dazu affine zweite Moleküle
KR20040010041A (ko) 2000-05-04 2004-01-31 예일 유니버시티 단백질 활성의 스크리닝을 위한 고밀도 단백질 어레이
GB0029729D0 (en) * 2000-12-06 2001-01-17 Ids Ltd Method for detection of vitamin D metabolites
EP1377827A2 (en) * 2001-04-10 2004-01-07 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen in Reutlingen Stiftung Bürgerlichen Rechts Method for determining analyte molecules in a sample
US20040248323A1 (en) 2003-06-09 2004-12-09 Protometrix, Inc. Methods for conducting assays for enzyme activity on protein microarrays
GB0421838D0 (en) 2004-09-30 2004-11-03 Congenia S R L Cancer markers
WO2006132666A1 (en) * 2005-06-06 2006-12-14 Decision Biomarkers, Inc. Assays based on liquid flow over arrays
GB201016403D0 (en) 2010-09-29 2010-11-10 Bath Ventures Novel interaction between staphylococcus aureus Sbi and C3d protiens
US9075021B2 (en) 2012-02-17 2015-07-07 Toyota Motor Engineering & Manufacturing North America, Inc. Methods and systems for monitoring content of coating solutions using automated titration devices
CN108139395A (zh) * 2015-10-16 2018-06-08 东洋纺株式会社 免疫层析试验片
WO2018148489A1 (en) * 2017-02-09 2018-08-16 Promega Corporation Analyte detection immunoassay

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0664059B2 (ja) * 1982-08-27 1994-08-22 マルティライト リミティド 被検体の濃度の測定方法
WO1988001058A1 (en) * 1986-08-06 1988-02-11 Roger Philip Ekins Determination of analyte concentration using two labelling markers
US5098846A (en) * 1987-05-27 1992-03-24 Mclean Hospital Solid phase protein assay by solid phase free site titration
GB8803000D0 (en) * 1988-02-10 1988-03-09 Ekins Roger Philip Determination of ambient concentrations of several analytes

Also Published As

Publication number Publication date
DE69411189D1 (de) 1998-07-23
EP0736175B1 (en) 1998-06-17
AU1321795A (en) 1995-07-17
JPH09512899A (ja) 1997-12-22
JP3424831B2 (ja) 2003-07-07
US5834319A (en) 1998-11-10
EP0736175A1 (en) 1996-10-09
GB9326451D0 (en) 1994-02-23
WO1995018376A1 (en) 1995-07-06
DE69411189T2 (de) 1998-11-19

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