JPH08510129A - B型肝炎ウイルス突然変異体、試薬及び検出方法 - Google Patents
B型肝炎ウイルス突然変異体、試薬及び検出方法Info
- Publication number
- JPH08510129A JPH08510129A JP6525598A JP52559894A JPH08510129A JP H08510129 A JPH08510129 A JP H08510129A JP 6525598 A JP6525598 A JP 6525598A JP 52559894 A JP52559894 A JP 52559894A JP H08510129 A JPH08510129 A JP H08510129A
- Authority
- JP
- Japan
- Prior art keywords
- hbv
- mutant
- antibody
- mutant hbv
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2730/00—Reverse transcribing DNA viruses
- C12N2730/00011—Details
- C12N2730/10011—Hepadnaviridae
- C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
- C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/82—Hepatitis associated antigens and antibodies
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.HBsAgゲノムの366位に少なくとも6つのヌクレオチドが挿入されて いる修飾“a”抗原決定基を含む精製突然変異HBVポリヌクレオチド。 2.前記6つのヌクレオチドが−A−A−C−A−C−A−である請求項1に記 載のポリヌクレオチド。 3.HBsAg配列の122位に少なくとも2つのアミノ酸が挿入されている修 飾“a”抗原決定基を含む組換え突然変異HBVポリヌクレオチド。 4.組換えベクター内に含まれている請求項3に記載のポリヌクレオチド。 5.更に、前記ベクターを用いて形質転換された宿主細胞を包含する請求項4に 記載のポリヌクレオチド。 6.前記122位に挿入されているアミノ酸がN及びTである請求項3に記載の ポリペプチド。 7.突然変異HBVゲノムから誘導されたDNAの読取り枠を含む組換え発現系 であって、前記ゲノムが、HBsAgゲノムの366位に少なくとも6つのヌク レオチドが挿入されている修飾“a”抗原決定基を含み、前記読取り枠が、所望 の宿主と適合性のある制御配列に操作可能に結合 されている組換え発現系。 8.更に、前記組換え発現系を用いて形質転換された細胞を包含する請求項7に 記載の発現系。 9.更に、前記細胞によって産生されたポリペプチドを包含する請求項8に記載 の発現系。 10.前記6つのヌクレオチドが−A−A−C−A−C−A−である請求項7に 記載の発現系。 11.突然変異HBVポリペプチドの調製物を包含する精製突然変異HBVであ って、前記突然変異HBVポリペプチドが、HBsAg配列の122位に少なく とも2つのアミノ酸が挿入されている修飾“a”抗原決定基を含んでおり、前記 122位に挿入されている2つのアミノ酸がN及びTである精製突然変異HBV 。 12.HBsAgゲノムの366位に少なくとも6つのヌクレオチドが挿入され ており、前記6つのヌクレオチドが−A−A−C−A−C−A−である修飾“a ”抗原決定基を含む突然変異HBVゲノムから誘導された配列を含む組換えポリ ペプチド。 13.少なくとも1つの突然変異HBVエピトープに対する抗体であって、前記 エピトープがHBsAg配列の12 2位に少なくとも2つのアミノ酸が挿入されている修飾“a”抗原決定基を含む 抗体。 14.前記抗体が、ポリクローナル抗体及びモノクローナル抗体からなる群から 選択される請求項13に記載の抗体。 15.前記122位に挿入されている2つのアミノ酸がN及びTである請求項1 3に記載の抗体。 16.突然変異HBVのポリペプチドを含む融合ポリペプチドであって、HBs Ag配列の122位に少なくとも2つのアミノ酸が挿入されている修飾“a”抗 原決定基を更に含む融合ポリペプチド。 17.前記122位に挿入されている2つのアミノ酸がN及びTである請求項1 6に記載の融合ポリペプチド。 18.真核性または原核性宿主内で宿主内で産生されたときに粒子を形成し得る アミノ酸配列を有する非HBVポリペプチドと突然変異HBVエピトープとを含 む、突然変異HBV感染に対して免疫原性である粒子であって、前記突然変異H BVの前記エピトープが更に、HBsAg配列の122位に少なくとも2つのア ミノ酸が挿入されており、前記122位に挿入されている2つのアミノ酸がN及 びTである修飾“a”抗原決定基を含む粒子。 19.HBsAgゲノムの366位に少なくとも6つのヌクレオチドが挿入され ている修飾“a”抗原決定基を含む、突然変異HBVに対するポリヌクレオチド プローブ。 20.前記6つのヌクレオチドが−A−A−C−A−C−A−である請求項19 に記載のポリヌクレオチドプローブ。 21.HBV抗原中に存在するHBVエピトープを有するポリペプチドを含む容 器を包含する、検査試料中の突然変異HBV抗原または抗体の存在を判定するた めのキットであって、前記エピトープが、HBsAg配列の122位に少なくと も2つのアミノ酸が挿入されている修飾“a”抗原決定基を含むキット。 22.前記ポリペプチドが固相に付着されている請求項21に記載のキット。 23.検出すべき突然変異HBV抗原に対する抗体を含む容器を包含する、検査 試料中の突然変異HBV抗原または抗体の存在を判定するためのキットであって 、前記抗原が、HBsAg配列の122位に少なくとも2つのアミノ酸が挿入さ れている修飾“a”抗原決定基を含むキット。 24.前記抗体が固相に付着されている請求項23に記載のキット。 25.突然変異HBV由来のヌクレオチド配列を含むポリヌクレオチドプローブ を含む容器を包含する、突然変異HBVポリヌクレオチドを含む疑いのある検査 試料中の該ポリヌクレオチドの存在を判定するためのキットであって、前記ヌク レオチド配列には、HBsAgゲノムの366位に少なくとも6つヌクレオチド が挿入されているキット。 26.HBsAg配列の122位に少なくとも2つのアミノ酸が挿入されている 修飾“a”抗原決定基を含む突然変異HBVエピトープを含むポリペプチドを製 造する方法であって、HBVエピトープを含むポリペプチドをコードする配列を 含む発現ベクターを用いて形質転換した宿主細胞を、前記ポリペプチドが発現し 得る条件下及び時間でインキュベートすることからなる方法。 27.HBVを含む疑いのある検査試料中の突然変異HBV核酸を検出する方法 であって、 a.検査試料を、HBsAgゲノムの366位に少なくとも6つのヌクレオチ ドが挿入されている突然変異HBV由来のヌクレオチド配列を含むHBVポリヌ クレオチド用プローブと、該プローブと検査試料中の突然変異HBV核酸とで複 合体を形成し得る条件下及び時間で反応させ; b.前記プローブを含む複合体を検出する ことからなる方法。 28.前記6つのヌクレオチドが−A−A−C−A−C−A−である請求項27 に記載の方法。 29.HBVを含む疑いのある検査試料中の突然変異HBV抗原を検出する方法 であって、 a.検査試料を、HBsAg配列の122位に少なくとも2つのアミノ酸が挿 入されている修飾“a”抗原決定基を含む検出すべき突然変異HBV抗原に対す る抗体と、抗体/抗原複合体を形成し得るに十分な時間及び条件下で接触させ; b.前記抗体を含む複合体を検出する ことからなる方法。 30.HBV抗体を含む疑いのある検査試料中のHBV抗体を検出する方法であ って、 a.検査試料を、HBsAg配列の122位に少なくとも2つのアミノ酸が挿 入されている修飾“a”抗原決定基を含む突然変異HBVエピトープを含むプロ ーブポリペプチドと、抗原/抗体複合体を形成し得るに十分な時間及び条件下で 接触させ; b.前記プローブポリペプチドを含む複合体を検出する ことからなる方法。 31.前記122位に挿入されている2つのアミノ酸がN及びTである請求項3 0に記載のポリペプチド。 32.医薬上容認可能な賦形剤中に、HBVエピトープを含む免疫原性突然変異 HBVポリペプチドを薬理上有効量含む突然変異HBV感染治療用ワクチンであ って、前記突然変異HBVエピトープが、HBsAg配列の122位に少なくと も2つのアミノ酸が挿入されている修飾“a”抗原決定基を含んでおり、前記1 22位に挿入されている2つのアミノ酸がN及びTであるワクチン。 33.医薬上容認可能な賦形剤中に、薬理上有効量の不活化または弱毒化突然変 異HBVを含む突然変異HBV感染治療用ワクチンであって、前記突然変異HB Vが、HBsAg配列の122位に少なくとも2つのアミノ酸が挿入されている 修飾“a”抗原決定基を含むワクチン。 34.突然変異HBVに感染させた組織培養増殖細胞であって、前記突然変異H BVが、HBsAg配列の122位に少なくとも2つのアミノ酸が挿入されてい る修飾“a”抗原決定基を含む組織培養増殖細胞。 35.突然変異HBVに対する抗体を製造する方法であって、免疫応答を生起す るのに十分な量の突然変異HBVエピトープを含む単離免疫原性ポリペプチドを 個体に投与することからなり、前記突然変異エピトープが、HBsAg配列の1 22位に少なくとも2つのアミノ酸が挿入されている修飾“a”抗原決定基を含 む方法。
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US08/059,031 US5595739A (en) | 1993-05-07 | 1993-05-07 | Hepatitis B virus mutants, reagents and methods for detection |
US08/059,031 | 1993-05-07 | ||
PCT/US1994/005090 WO1994026904A1 (en) | 1993-05-07 | 1994-05-09 | Hepatitis b virus mutants, reagents and methods for detection |
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JPH08510129A true JPH08510129A (ja) | 1996-10-29 |
JP3813168B2 JP3813168B2 (ja) | 2006-08-23 |
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JP52559894A Expired - Lifetime JP3813168B2 (ja) | 1993-05-07 | 1994-05-09 | B型肝炎ウイルス突然変異体、試薬及び検出方法 |
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US (4) | US5595739A (ja) |
EP (1) | EP0745128B8 (ja) |
JP (1) | JP3813168B2 (ja) |
AU (1) | AU6908294A (ja) |
CA (1) | CA2162132C (ja) |
DE (1) | DE69433285T2 (ja) |
ES (1) | ES2212796T3 (ja) |
WO (1) | WO1994026904A1 (ja) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007282639A (ja) * | 1995-04-17 | 2007-11-01 | Solexa Inc | 平行オリゴヌクレオチド伸長によるdna配列決定 |
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Families Citing this family (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5595739A (en) * | 1993-05-07 | 1997-01-21 | Abbott Laboratories | Hepatitis B virus mutants, reagents and methods for detection |
US5879656A (en) * | 1993-10-26 | 1999-03-09 | Thomas Jefferson University | Methods of treating metastatic colorectal cancer with ST receptor binding compounds |
US7097839B1 (en) * | 1993-10-26 | 2006-08-29 | Thomas Jefferson University | ST receptor binding compounds and methods of using the same |
GB9401987D0 (en) * | 1994-02-02 | 1994-03-30 | Imperial College | Modified nucleic acid |
ZA973367B (en) | 1996-04-19 | 1997-11-18 | Innogenetics Nv | Method for typing and detecting HBV. |
USRE40233E1 (en) | 1996-11-08 | 2008-04-08 | Melbourne Health | Viral variants and methods for detecting same |
AUPO351996A0 (en) * | 1996-11-08 | 1996-12-05 | Western Health Care Network | Viral variants and methods for detecting same |
EP0919568A1 (en) | 1997-12-01 | 1999-06-02 | Sorin Diagnostics S.r.l. | Escape mutant of the surface antigen of hepatitis B virus |
BR9913333A (pt) | 1998-08-25 | 2001-05-15 | Univ Yale | Inibição e tratamento do vìrus da hepatite b e flavivìrus com helioxatina e seus análogos |
AUPP706098A0 (en) * | 1998-11-11 | 1998-12-03 | North Western Health Care Network | Biological compositions, components thereof and uses therefor |
CZ20012428A3 (cs) * | 1998-12-31 | 2001-11-14 | Aventis Pharma S. A. | Způsob separace virových částic |
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US6670167B1 (en) * | 1999-11-01 | 2003-12-30 | Agouron Pharmaceuticals, Inc. | Catalytic domain of the human effector cell cycle checkpoint protein kinase materials and methods for identification of inhibitors thereof |
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SG90149A1 (en) * | 2000-07-18 | 2002-07-23 | Government Of The Republic Of | Diagnostic assay |
US6583279B1 (en) | 2001-01-26 | 2003-06-24 | Becton, Dickinson And Company | Sequences and methods for detection of hepatitis B virus |
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NZ527687A (en) * | 2001-05-11 | 2005-10-28 | Wellstat Biolog Corp Formerly | Preferential binding of an oncolytic virus such as Newcastle Disease Virus (NDV) to leukocytes compared with erythrocytes used as a therapy for cancer |
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US8278432B2 (en) | 2002-03-29 | 2012-10-02 | Innogenetics N.V. | HBV drug resistance methods |
WO2003087351A1 (en) | 2002-04-12 | 2003-10-23 | Melbourne Health | Hepatitis b viral variants with redused susceptibility to nucleoside analogs and uses thereof |
US20030219407A1 (en) * | 2002-05-15 | 2003-11-27 | The Regents Of The University Of California | RNA silencing in animals as an antiviral defense |
WO2004002459A1 (ja) * | 2002-06-28 | 2004-01-08 | Japan Science And Technology Agency | システイン残基を改変したタンパク質からなる中空ナノ粒子およびそれを用いた薬剤 |
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JP2008537483A (ja) | 2005-03-15 | 2008-09-18 | イノジェネティックス・ナムローゼ・フェンノートシャップ | ヌクレオシドアナログに対する感受性を低減したb型肝炎ウイルス変種およびその使用 |
US8211443B2 (en) * | 2005-04-08 | 2012-07-03 | Melbourne Health | Variants of hepatitis B virus with resistance to anti-viral nucleoside agents and applications thereof |
JP2008534020A (ja) * | 2005-04-08 | 2008-08-28 | メルボルン ヘルス | 抗ウイルス性のヌクレオシド剤に耐性を示すb型肝炎ウイルスのバリアントおよびその応用法 |
US20100136520A1 (en) | 2007-09-13 | 2010-06-03 | Abbott Laboratories | Detecting hepatitis b virus |
US20090098531A1 (en) * | 2007-09-13 | 2009-04-16 | Abbott Laboratories | Detecting hepatitis b virus |
US8138318B2 (en) | 2007-09-13 | 2012-03-20 | Abbott Laboratories | Hepatitis B pre-S2 nucleic acid |
EP2379738B1 (en) * | 2008-12-31 | 2017-01-25 | 3M Innovative Properties Company | Coliform detection process |
CA3149557A1 (en) | 2019-09-30 | 2021-04-08 | Scott J. Balsitis | Hbv vaccines and methods treating hbv |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2597606B1 (fr) * | 1986-04-16 | 1989-10-06 | Pasteur Institut | Nouveau reactif pour la detection d'hepatites virales non a non b et procede de diagnostic de telles hepatites par voie immunoenzymatique |
US5565354A (en) * | 1986-09-05 | 1996-10-15 | Sandoz Ltd. | Production of human monoclonal antibodies specific for hepatitis B surface antigen |
GB9007024D0 (en) * | 1990-03-29 | 1990-05-30 | Imperial College | Novel vaccine |
IT1241154B (it) * | 1990-05-18 | 1993-12-29 | Slavo | Metodo e composizione reagente per la determinazione dell'alanina amminotrasferasi e dell'antigene hbsag in uno stesso campione biologico |
CA2067003A1 (en) * | 1991-04-29 | 1992-10-30 | Peter J. Kniskern | Hbsag escape mutant vaccine |
US5595739A (en) * | 1993-05-07 | 1997-01-21 | Abbott Laboratories | Hepatitis B virus mutants, reagents and methods for detection |
GB9401987D0 (en) * | 1994-02-02 | 1994-03-30 | Imperial College | Modified nucleic acid |
-
1993
- 1993-05-07 US US08/059,031 patent/US5595739A/en not_active Expired - Lifetime
-
1994
- 1994-05-09 JP JP52559894A patent/JP3813168B2/ja not_active Expired - Lifetime
- 1994-05-09 DE DE69433285T patent/DE69433285T2/de not_active Expired - Lifetime
- 1994-05-09 WO PCT/US1994/005090 patent/WO1994026904A1/en active IP Right Grant
- 1994-05-09 ES ES94917325T patent/ES2212796T3/es not_active Expired - Lifetime
- 1994-05-09 CA CA002162132A patent/CA2162132C/en not_active Expired - Lifetime
- 1994-05-09 AU AU69082/94A patent/AU6908294A/en not_active Abandoned
- 1994-05-09 EP EP94917325A patent/EP0745128B8/en not_active Expired - Lifetime
-
1995
- 1995-05-23 US US08/450,942 patent/US5925512A/en not_active Expired - Lifetime
- 1995-05-23 US US08/450,943 patent/US5593825A/en not_active Expired - Lifetime
- 1995-05-23 US US08/447,591 patent/US5591440A/en not_active Expired - Lifetime
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007282639A (ja) * | 1995-04-17 | 2007-11-01 | Solexa Inc | 平行オリゴヌクレオチド伸長によるdna配列決定 |
JP2013143952A (ja) * | 2002-06-14 | 2013-07-25 | Gen Probe Inc | B型肝炎ウイルスを検出するための組成物および方法 |
US9914982B2 (en) | 2002-06-14 | 2018-03-13 | Gen-Probe Incorporated | Compositions and methods for detecting hepatitis B virus |
Also Published As
Publication number | Publication date |
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AU6908294A (en) | 1994-12-12 |
WO1994026904A1 (en) | 1994-11-24 |
JP3813168B2 (ja) | 2006-08-23 |
CA2162132C (en) | 2001-12-11 |
CA2162132A1 (en) | 1994-11-24 |
US5593825A (en) | 1997-01-14 |
DE69433285T2 (de) | 2004-06-24 |
ES2212796T3 (es) | 2004-08-01 |
US5925512A (en) | 1999-07-20 |
DE69433285D1 (de) | 2003-12-04 |
EP0745128B8 (en) | 2004-07-14 |
EP0745128B1 (en) | 2003-10-29 |
EP0745128A4 (en) | 2000-01-12 |
US5591440A (en) | 1997-01-07 |
EP0745128A1 (en) | 1996-12-04 |
US5595739A (en) | 1997-01-21 |
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