JPH0260316B2 - - Google Patents
Info
- Publication number
- JPH0260316B2 JPH0260316B2 JP57218115A JP21811582A JPH0260316B2 JP H0260316 B2 JPH0260316 B2 JP H0260316B2 JP 57218115 A JP57218115 A JP 57218115A JP 21811582 A JP21811582 A JP 21811582A JP H0260316 B2 JPH0260316 B2 JP H0260316B2
- Authority
- JP
- Japan
- Prior art keywords
- kallikrein
- sodium chloride
- column
- acid
- activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108060005987 Kallikrein Proteins 0.000 claims description 58
- 102000001399 Kallikrein Human genes 0.000 claims description 58
- 238000000034 method Methods 0.000 claims description 23
- 239000003463 adsorbent Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 6
- 125000006850 spacer group Chemical group 0.000 claims description 6
- VHRGRCVQAFMJIZ-UHFFFAOYSA-N cadaverine Chemical compound NCCCCCN VHRGRCVQAFMJIZ-UHFFFAOYSA-N 0.000 claims description 4
- MFLYTCSVJYYAOR-UHFFFAOYSA-N (carbamimidoylazaniumyl)formate Chemical compound NC(=N)NC(O)=O MFLYTCSVJYYAOR-UHFFFAOYSA-N 0.000 claims description 3
- PWGJDPKCLMLPJW-UHFFFAOYSA-N 1,8-diaminooctane Chemical compound NCCCCCCCCN PWGJDPKCLMLPJW-UHFFFAOYSA-N 0.000 claims description 2
- YQLZOAVZWJBZSY-UHFFFAOYSA-N decane-1,10-diamine Chemical compound NCCCCCCCCCCN YQLZOAVZWJBZSY-UHFFFAOYSA-N 0.000 claims description 2
- NAQMVNRVTILPCV-UHFFFAOYSA-N hexane-1,6-diamine Chemical group NCCCCCCN NAQMVNRVTILPCV-UHFFFAOYSA-N 0.000 claims description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 52
- 239000011780 sodium chloride Substances 0.000 description 26
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 20
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 18
- 239000000872 buffer Substances 0.000 description 18
- 230000000694 effects Effects 0.000 description 16
- BPMFZUMJYQTVII-UHFFFAOYSA-N guanidinoacetic acid Chemical compound NC(=N)NCC(O)=O BPMFZUMJYQTVII-UHFFFAOYSA-N 0.000 description 15
- 239000000243 solution Substances 0.000 description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 108090000317 Chymotrypsin Proteins 0.000 description 6
- 229920002684 Sepharose Polymers 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 229960002376 chymotrypsin Drugs 0.000 description 6
- 108090000631 Trypsin Proteins 0.000 description 5
- 102000004142 Trypsin Human genes 0.000 description 5
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 5
- 239000012588 trypsin Substances 0.000 description 5
- FCEQRBOTYXIORK-UHFFFAOYSA-N 2-(diaminomethylideneamino)benzoic acid Chemical compound NC(=N)NC1=CC=CC=C1C(O)=O FCEQRBOTYXIORK-UHFFFAOYSA-N 0.000 description 4
- 108010093008 Kinins Proteins 0.000 description 4
- 102000002397 Kinins Human genes 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 238000010828 elution Methods 0.000 description 4
- GSPZXGHHFDORDC-UHFFFAOYSA-N 2-(diaminomethylideneamino)butanoic acid Chemical compound CCC(C(O)=O)N=C(N)N GSPZXGHHFDORDC-UHFFFAOYSA-N 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 3
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 125000003277 amino group Chemical group 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- TUHVEAJXIMEOSA-UHFFFAOYSA-N gamma-guanidinobutyric acid Natural products NC(=[NH2+])NCCCC([O-])=O TUHVEAJXIMEOSA-UHFFFAOYSA-N 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 125000002795 guanidino group Chemical group C(N)(=N)N* 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- RSYYQCDERUOEFI-UHFFFAOYSA-N 2-benzamido-5-(diaminomethylideneazaniumyl)pentanoate Chemical compound NC(N)=NCCCC(C(O)=O)NC(=O)C1=CC=CC=C1 RSYYQCDERUOEFI-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- -1 aminohexyl Chemical group 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 150000001483 arginine derivatives Chemical class 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229940062190 pancreas extract Drugs 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000002336 sorption--desorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000000304 vasodilatating effect Effects 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21811582A JPS59109172A (ja) | 1982-12-13 | 1982-12-13 | カリクレインの精製法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21811582A JPS59109172A (ja) | 1982-12-13 | 1982-12-13 | カリクレインの精製法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS59109172A JPS59109172A (ja) | 1984-06-23 |
| JPH0260316B2 true JPH0260316B2 (pt) | 1990-12-14 |
Family
ID=16714854
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP21811582A Granted JPS59109172A (ja) | 1982-12-13 | 1982-12-13 | カリクレインの精製法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS59109172A (pt) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05121958A (ja) * | 1991-10-29 | 1993-05-18 | Saitama Nippon Denki Kk | 直線増幅装置の歪補償制御方式 |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ216094A (en) * | 1985-05-15 | 1989-06-28 | Commw Serum Lab Commission | Method for purification of an immunoglobulin |
| WO1987002709A1 (en) * | 1985-10-24 | 1987-05-07 | Biotechnology Research Partners, Ltd. | Blood tests diagnostic for hypertension |
| JP4869856B2 (ja) * | 2006-10-04 | 2012-02-08 | オリンパス株式会社 | 電気回路装置及びその製造方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS504227A (pt) * | 1972-12-19 | 1975-01-17 | ||
| JPS5276481A (en) * | 1975-12-18 | 1977-06-27 | Otsuka Pharmaceut Co Ltd | Production of high purity urokinase |
| JPS5592355A (en) * | 1978-12-22 | 1980-07-12 | Nippon Soda Co Ltd | Enzyme-adsorbing substance and purification of enzyme using the same |
-
1982
- 1982-12-13 JP JP21811582A patent/JPS59109172A/ja active Granted
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS504227A (pt) * | 1972-12-19 | 1975-01-17 | ||
| JPS5276481A (en) * | 1975-12-18 | 1977-06-27 | Otsuka Pharmaceut Co Ltd | Production of high purity urokinase |
| JPS5592355A (en) * | 1978-12-22 | 1980-07-12 | Nippon Soda Co Ltd | Enzyme-adsorbing substance and purification of enzyme using the same |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05121958A (ja) * | 1991-10-29 | 1993-05-18 | Saitama Nippon Denki Kk | 直線増幅装置の歪補償制御方式 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS59109172A (ja) | 1984-06-23 |
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