JPH0160781B2 - - Google Patents
Info
- Publication number
- JPH0160781B2 JPH0160781B2 JP58057415A JP5741583A JPH0160781B2 JP H0160781 B2 JPH0160781 B2 JP H0160781B2 JP 58057415 A JP58057415 A JP 58057415A JP 5741583 A JP5741583 A JP 5741583A JP H0160781 B2 JPH0160781 B2 JP H0160781B2
- Authority
- JP
- Japan
- Prior art keywords
- specific cleavage
- base
- cleavage product
- product
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000003776 cleavage reaction Methods 0.000 claims description 83
- 230000007017 scission Effects 0.000 claims description 83
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 58
- 238000000926 separation method Methods 0.000 claims description 29
- 239000000126 substance Substances 0.000 claims description 28
- 238000000354 decomposition reaction Methods 0.000 claims description 24
- 239000000203 mixture Substances 0.000 claims description 23
- 238000005070 sampling Methods 0.000 claims description 23
- 238000012545 processing Methods 0.000 claims description 21
- 238000000376 autoradiography Methods 0.000 claims description 18
- 238000011161 development Methods 0.000 claims description 17
- 238000003672 processing method Methods 0.000 claims description 17
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 14
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims description 14
- 230000005855 radiation Effects 0.000 claims description 13
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 12
- 229940104302 cytosine Drugs 0.000 claims description 7
- 229930024421 Adenine Natural products 0.000 claims description 6
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 6
- 229960000643 adenine Drugs 0.000 claims description 6
- 229940113082 thymine Drugs 0.000 claims description 6
- 230000002285 radioactive effect Effects 0.000 claims description 5
- 238000009499 grossing Methods 0.000 claims description 2
- 239000000047 product Substances 0.000 claims 25
- 230000015556 catabolic process Effects 0.000 claims 1
- 239000007857 degradation product Substances 0.000 claims 1
- 238000006731 degradation reaction Methods 0.000 claims 1
- 238000000034 method Methods 0.000 description 30
- 238000004020 luminiscence type Methods 0.000 description 13
- 230000006870 function Effects 0.000 description 12
- 238000010586 diagram Methods 0.000 description 7
- 230000003287 optical effect Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- -1 europium-activated barium fluoride bromide Chemical class 0.000 description 4
- 230000005284 excitation Effects 0.000 description 4
- 230000003321 amplification Effects 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 229910052693 Europium Inorganic materials 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 238000009125 cardiac resynchronization therapy Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229920000139 polyethylene terephthalate Polymers 0.000 description 2
- 239000005020 polyethylene terephthalate Substances 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 239000000941 radioactive substance Substances 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 1
- 150000001342 alkaline earth metals Chemical class 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000010365 information processing Effects 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 229920006352 transparent thermoplastic Polymers 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01T—MEASUREMENT OF NUCLEAR OR X-RADIATION
- G01T1/00—Measuring X-radiation, gamma radiation, corpuscular radiation, or cosmic radiation
- G01T1/16—Measuring radiation intensity
- G01T1/20—Measuring radiation intensity with scintillation detectors
- G01T1/2012—Measuring radiation intensity with scintillation detectors using stimulable phosphors, e.g. stimulable phosphor sheets
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Physics & Mathematics (AREA)
- High Energy & Nuclear Physics (AREA)
- Molecular Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Measurement Of Radiation (AREA)
- Radiography Using Non-Light Waves (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58057415A JPS59182362A (ja) | 1983-03-31 | 1983-03-31 | オ−トラジオグラフイ−における信号処理方法 |
| EP84100150A EP0113677A3 (de) | 1983-01-08 | 1984-01-09 | Signalbearbeitungsmethode in der Autoradiographie |
| US06/883,922 US4837733A (en) | 1983-01-08 | 1986-07-10 | Signal processing method in autoradiography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58057415A JPS59182362A (ja) | 1983-03-31 | 1983-03-31 | オ−トラジオグラフイ−における信号処理方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS59182362A JPS59182362A (ja) | 1984-10-17 |
| JPH0160781B2 true JPH0160781B2 (de) | 1989-12-25 |
Family
ID=13055015
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58057415A Granted JPS59182362A (ja) | 1983-01-08 | 1983-03-31 | オ−トラジオグラフイ−における信号処理方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS59182362A (de) |
-
1983
- 1983-03-31 JP JP58057415A patent/JPS59182362A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS59182362A (ja) | 1984-10-17 |
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