JPH01501339A - 改良された核酸ハイブリダイゼーション方法及びそれに用いるキット - Google Patents

改良された核酸ハイブリダイゼーション方法及びそれに用いるキット

Info

Publication number
JPH01501339A
JPH01501339A JP62506425A JP50642587A JPH01501339A JP H01501339 A JPH01501339 A JP H01501339A JP 62506425 A JP62506425 A JP 62506425A JP 50642587 A JP50642587 A JP 50642587A JP H01501339 A JPH01501339 A JP H01501339A
Authority
JP
Japan
Prior art keywords
probe
probes
binding
polynucleotide
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP62506425A
Other languages
English (en)
Japanese (ja)
Inventor
ビーブ、ジョウン　マリリン
グランビル、リンダ　リー
リアリー、ジェフリー　ジョゼフ
ライス、エドワード　グレイ
Original Assignee
ベックマン　インスツルメンツ　インコーポレーテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ベックマン　インスツルメンツ　インコーポレーテッド filed Critical ベックマン　インスツルメンツ　インコーポレーテッド
Publication of JPH01501339A publication Critical patent/JPH01501339A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP62506425A 1986-10-14 1987-10-06 改良された核酸ハイブリダイゼーション方法及びそれに用いるキット Pending JPH01501339A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US91920186A 1986-10-14 1986-10-14
US919,201 1986-10-14

Publications (1)

Publication Number Publication Date
JPH01501339A true JPH01501339A (ja) 1989-05-11

Family

ID=25441695

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62506425A Pending JPH01501339A (ja) 1986-10-14 1987-10-06 改良された核酸ハイブリダイゼーション方法及びそれに用いるキット

Country Status (7)

Country Link
JP (1) JPH01501339A (fr)
AU (1) AU8103687A (fr)
CA (1) CA1309932C (fr)
DK (1) DK324088D0 (fr)
FI (1) FI882800A (fr)
NO (1) NO882593D0 (fr)
WO (1) WO1988002785A2 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU593151B2 (en) * 1986-11-12 1990-02-01 Molecular Diagnostics, Inc. Method for the detection of nucleic acid hybrids
DE3888653T2 (de) * 1987-12-21 1994-07-07 Applied Biosystems Verfahren und Testsatz zum Nachweis einer Nukleinsäure-Sequenz.
US5354657A (en) * 1988-01-12 1994-10-11 Boehringer Mannheim Gmbh Process for the highly specific detection of nucleic acids in solid
JP2897959B2 (ja) * 1988-05-20 1999-05-31 エフ．ホフマン―ラ ロシュ アクチェンゲゼルシャフト 固定化された配列特異的プローブ
GB2225112A (en) * 1988-11-22 1990-05-23 Ici Plc Hybridisation probes
JP3310662B2 (ja) * 2000-03-31 2002-08-05 三光純薬株式会社 プローブポリマー作製用プローブ、プローブポリマーの作製方法及びその利用
BRPI0412849A (pt) 2003-07-25 2006-09-26 Idenix Cayman Ltd compostos, composições e usos de análogos de nucleosìdeo de purina para o tratamento de flaviviridae, incluindo hepatite c
WO2005071401A2 (fr) * 2004-01-15 2005-08-04 Chiron Corporation Dosage multiplex homogene pour des cibles d'acides nucleiques
CN103733047B (zh) 2011-08-11 2016-03-30 奥林巴斯株式会社 目标粒子的检测方法
WO2013031365A1 (fr) 2011-08-30 2013-03-07 オリンパス株式会社 Procédé de détection de particules cibles
JP5940644B2 (ja) 2012-02-22 2016-06-29 オリンパス株式会社 標的粒子の検出方法
EP2829614A4 (fr) * 2012-03-21 2016-03-16 Olympus Corp Procédé de détection d'une molécule d'acide nucléique cible

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2057685B (en) * 1979-03-19 1983-12-21 Int Diagnostic Tech Double tagged immunoassay
US4487830A (en) * 1982-05-14 1984-12-11 American Hoechst Corporation Enzyme/immunofluorescent assay for autoantibodies
CA1222680A (fr) * 1983-07-05 1987-06-09 Nanibhushan Dattagupta Dosages de sequences de nucleotides particulieres dans des echantillons d'adn
NO843838L (no) * 1983-09-26 1985-03-27 Ortho Diagnostic Systems Inc Fremgangsmaate for detektering av nukleinsyre
CA1260372A (fr) * 1984-04-27 1989-09-26 Elazar Rabbani Methode d'hybridation pour la detection de materiaux genetiques
US4820630A (en) * 1984-11-23 1989-04-11 Digene Diagnostics, Incorporated Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels
GB8432118D0 (en) * 1984-12-19 1985-01-30 Malcolm A D B Sandwich hybridisation technique
CA1272443A (fr) * 1985-02-22 1990-08-07 Nanibhushan Dattagupta Essai de double hybridation en solution-phase pour deceler des sequences polynucleotidiques
GB8508431D0 (en) * 1985-04-01 1985-05-09 English Clays Lovering Pochin Paper coating apparatus
GB8509367D0 (en) * 1985-04-12 1985-05-15 Amersham Int Plc Nucleic acid hybridisation
ES8707343A1 (es) * 1985-06-13 1987-07-16 Amgen Un metodo para aislar una secuencia de acido nucleico objetivo seleccionada
EP0238332A2 (fr) * 1986-03-19 1987-09-23 Cetus Corporation Méthode d'hybridation en phase liquide et trousse de réactifs pour détecter la présence de séquences d'acides nucléiques dans des échantillons
GB8607101D0 (en) * 1986-03-21 1986-04-30 Serono Diagnostics Ltd Immunoassay

Also Published As

Publication number Publication date
CA1309932C (fr) 1992-11-10
AU8103687A (en) 1988-05-06
FI882800A0 (fi) 1988-06-13
NO882593L (no) 1988-06-13
WO1988002785A2 (fr) 1988-04-21
DK324088D0 (da) 1988-06-14
FI882800A (fi) 1988-06-13
NO882593D0 (no) 1988-06-13
WO1988002785A3 (fr) 1988-07-14

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