JP7846763B2 - 変異型ニトリルヒドラターゼ、該変異型ニトリルヒドラターゼをコードする核酸、該核酸を含むベクター及び形質転換体、該変異型ニトリルヒドラターゼの製造方法、並びにアミド化合物の製造方法 - Google Patents

変異型ニトリルヒドラターゼ、該変異型ニトリルヒドラターゼをコードする核酸、該核酸を含むベクター及び形質転換体、該変異型ニトリルヒドラターゼの製造方法、並びにアミド化合物の製造方法

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Publication number
JP7846763B2
JP7846763B2 JP2024530676A JP2024530676A JP7846763B2 JP 7846763 B2 JP7846763 B2 JP 7846763B2 JP 2024530676 A JP2024530676 A JP 2024530676A JP 2024530676 A JP2024530676 A JP 2024530676A JP 7846763 B2 JP7846763 B2 JP 7846763B2
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amino acid
acid residue
subunit
terminus
nitrile hydratase
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JPWO2024004661A1 (https=
Inventor
大資 望月
淳子 徳田
泰史 喜田
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Mitsui Chemicals Inc
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Mitsui Chemicals Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01084—Nitrile hydratase (4.2.1.84)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2024530676A 2022-06-30 2023-06-14 変異型ニトリルヒドラターゼ、該変異型ニトリルヒドラターゼをコードする核酸、該核酸を含むベクター及び形質転換体、該変異型ニトリルヒドラターゼの製造方法、並びにアミド化合物の製造方法 Active JP7846763B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2022106069 2022-06-30
JP2022106069 2022-06-30
PCT/JP2023/022166 WO2024004661A1 (ja) 2022-06-30 2023-06-14 変異型ニトリルヒドラターゼ、該変異型ニトリルヒドラターゼをコードする核酸、該核酸を含むベクター及び形質転換体、該変異型ニトリルヒドラターゼの製造方法、並びにアミド化合物の製造方法

Publications (2)

Publication Number Publication Date
JPWO2024004661A1 JPWO2024004661A1 (https=) 2024-01-04
JP7846763B2 true JP7846763B2 (ja) 2026-04-15

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JP2024530676A Active JP7846763B2 (ja) 2022-06-30 2023-06-14 変異型ニトリルヒドラターゼ、該変異型ニトリルヒドラターゼをコードする核酸、該核酸を含むベクター及び形質転換体、該変異型ニトリルヒドラターゼの製造方法、並びにアミド化合物の製造方法

Country Status (6)

Country Link
EP (1) EP4549571A1 (https=)
JP (1) JP7846763B2 (https=)
KR (1) KR20250011936A (https=)
CN (1) CN119343452A (https=)
TW (1) TW202409063A (https=)
WO (1) WO2024004661A1 (https=)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004194588A (ja) 2002-12-19 2004-07-15 Mitsui Chemicals Inc 新規なニトリルヒドラターゼ
WO2007043466A1 (ja) 2005-10-07 2007-04-19 Mitsui Chemicals, Inc. アミド化合物の製造方法
JP2008506397A (ja) 2004-07-19 2008-03-06 チバ　スペシャルティ　ケミカルズ　ウォーター　トリートメント　リミテッド モノマー及びそのポリマーを調製する方法
WO2010055666A1 (ja) 2008-11-14 2010-05-20 三井化学株式会社 ニトリルヒドラターゼ変異体
WO2018124247A1 (ja) 2016-12-28 2018-07-05 三井化学株式会社 変異型ニトリルヒドラターゼ、該変異型ニトリルヒドラターゼをコードする核酸、該核酸を含む発現ベクター及び形質転換体、該変異型ニトリルヒドラターゼの製造方法、並びにアミド化合物の製造方法
JP2019176835A (ja) 2018-03-30 2019-10-17 三井化学株式会社 アミド化合物の製造方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3408737B2 (ja) 1998-03-16 2003-05-19 三井化学株式会社 ニトリルヒドラターゼの活性化に関与するタンパク質及びそれをコードする遺伝子
JP2022106069A (ja) 2021-01-06 2022-07-19 Ａｓｓｅｓｔ株式会社 動物意思判別プログラム

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004194588A (ja) 2002-12-19 2004-07-15 Mitsui Chemicals Inc 新規なニトリルヒドラターゼ
JP2008506397A (ja) 2004-07-19 2008-03-06 チバ　スペシャルティ　ケミカルズ　ウォーター　トリートメント　リミテッド モノマー及びそのポリマーを調製する方法
WO2007043466A1 (ja) 2005-10-07 2007-04-19 Mitsui Chemicals, Inc. アミド化合物の製造方法
WO2010055666A1 (ja) 2008-11-14 2010-05-20 三井化学株式会社 ニトリルヒドラターゼ変異体
WO2018124247A1 (ja) 2016-12-28 2018-07-05 三井化学株式会社 変異型ニトリルヒドラターゼ、該変異型ニトリルヒドラターゼをコードする核酸、該核酸を含む発現ベクター及び形質転換体、該変異型ニトリルヒドラターゼの製造方法、並びにアミド化合物の製造方法
JP2019176835A (ja) 2018-03-30 2019-10-17 三井化学株式会社 アミド化合物の製造方法

Also Published As

Publication number Publication date
CN119343452A (zh) 2025-01-21
KR20250011936A (ko) 2025-01-22
EP4549571A1 (en) 2025-05-07
WO2024004661A1 (ja) 2024-01-04
JPWO2024004661A1 (https=) 2024-01-04
TW202409063A (zh) 2024-03-01

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