JP7744026B2 - スプリットインテインシステムを使用したタンパク質精製 - Google Patents
スプリットインテインシステムを使用したタンパク質精製Info
- Publication number
- JP7744026B2 JP7744026B2 JP2022526270A JP2022526270A JP7744026B2 JP 7744026 B2 JP7744026 B2 JP 7744026B2 JP 2022526270 A JP2022526270 A JP 2022526270A JP 2022526270 A JP2022526270 A JP 2022526270A JP 7744026 B2 JP7744026 B2 JP 7744026B2
- Authority
- JP
- Japan
- Prior art keywords
- intein
- protein
- solid phase
- poi
- split
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/545—IL-1
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
- C07K17/02—Peptides being immobilised on, or in, an organic carrier
- C07K17/10—Peptides being immobilised on, or in, an organic carrier the carrier being a carbohydrate
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
- C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2025101380A JP2025133756A (ja) | 2019-11-22 | 2025-06-17 | スプリットインテインシステムを使用したタンパク質精製 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1917046.3 | 2019-11-22 | ||
| GBGB1917046.3A GB201917046D0 (en) | 2019-11-22 | 2019-11-22 | Improved protein production |
| PCT/EP2020/082966 WO2021099607A1 (en) | 2019-11-22 | 2020-11-20 | Protein purification using a split intein system |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2025101380A Division JP2025133756A (ja) | 2019-11-22 | 2025-06-17 | スプリットインテインシステムを使用したタンパク質精製 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2023502335A JP2023502335A (ja) | 2023-01-24 |
| JP2023502335A5 JP2023502335A5 (https=) | 2023-11-30 |
| JP7744026B2 true JP7744026B2 (ja) | 2025-09-25 |
Family
ID=69137378
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022526270A Active JP7744026B2 (ja) | 2019-11-22 | 2020-11-20 | スプリットインテインシステムを使用したタンパク質精製 |
| JP2025101380A Pending JP2025133756A (ja) | 2019-11-22 | 2025-06-17 | スプリットインテインシステムを使用したタンパク質精製 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2025101380A Pending JP2025133756A (ja) | 2019-11-22 | 2025-06-17 | スプリットインテインシステムを使用したタンパク質精製 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20240132538A1 (https=) |
| EP (1) | EP4061932A1 (https=) |
| JP (2) | JP7744026B2 (https=) |
| KR (1) | KR20220105157A (https=) |
| CN (2) | CN114698379B (https=) |
| CA (1) | CA3155170A1 (https=) |
| GB (1) | GB201917046D0 (https=) |
| WO (1) | WO2021099607A1 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4126899A1 (en) * | 2020-03-25 | 2023-02-08 | Merck Patent GmbH | Reuse of intein-bound resins for protein purification |
| KR20240007210A (ko) | 2021-05-12 | 2024-01-16 | 씨티바 바이오프로세스 알&디 에이비 | 개선된 단백질 정제 |
| CN114606252A (zh) * | 2022-04-20 | 2022-06-10 | 广州市乾相生物科技有限公司 | 一种基于过滤法的寡肽合成及纯化方法 |
| CN115028741A (zh) * | 2022-06-21 | 2022-09-09 | 苏州工业园区唯可达生物科技有限公司 | 一种肿瘤抗原抗体复合物及制备方法和应用 |
| CN115925830B (zh) * | 2022-08-15 | 2023-11-07 | 广州市乾相生物科技有限公司 | 一种内含肽变体及其在生物法制备类蛇毒肽前体中的应用 |
| AU2024270243A1 (en) | 2023-05-08 | 2025-11-06 | Cytiva Bioprocess R&D Ab | Split inteins for affinity capture |
| EP4726045A1 (en) | 2023-06-07 | 2026-04-15 | Peking University Third Hospital (The Third Clinical Medical School of Peking University) | Expression cassette combination and use thereof |
| EP4724477A1 (en) | 2023-06-11 | 2026-04-15 | Regeneron Pharmaceuticals, Inc. | Circularized antibody molecules |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014528720A (ja) | 2011-09-28 | 2014-10-30 | エラ、ビオテック、ソシエダッド、アノニマEra Biotech, S.A. | スプリットインテインおよびその使用 |
| JP2015522020A (ja) | 2012-06-27 | 2015-08-03 | ザ・トラスティーズ・オブ・プリンストン・ユニバーシティThe Trustees Of Princeton University | スプリットインテイン、複合体およびそれらの使用 |
| JP2016504417A (ja) | 2013-01-11 | 2016-02-12 | ザ テキサス エー アンド エム ユニヴァーシティー システムThe Texas A&M University System | インテイン媒介によるタンパク質の精製 |
| US20160207965A1 (en) | 2015-01-09 | 2016-07-21 | Ohio State Innovation Foundation | Protein production systems and methods thereof |
| JP2016534373A (ja) | 2013-10-09 | 2016-11-04 | ピュリディファイ リミテッド | クロマトグラフィー担体 |
| JP2017533701A (ja) | 2014-11-03 | 2017-11-16 | メルク パテント ゲーエムベーハー | 可溶性インテイン融合タンパク質、及び生体分子を精製する方法 |
| JP2017537632A (ja) | 2014-12-17 | 2017-12-21 | ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ | 改変κ軽鎖結合ポリペプチド |
| JP2019506163A (ja) | 2016-01-29 | 2019-03-07 | ザ・トラスティーズ・オブ・プリンストン・ユニバーシティThe Trustees Of Princeton University | 例外的なスプライシング活性を有するスプリットインテイン |
| JP2019524088A (ja) | 2016-07-06 | 2019-09-05 | アミコージェン・インコーポレイテッド | アルカリ耐性が向上した変異型免疫グロブリン結合タンパク質 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014056199A1 (zh) * | 2012-10-12 | 2014-04-17 | 清华大学 | 多肽的产生和纯化方法 |
| CN105316353A (zh) * | 2015-02-13 | 2016-02-10 | 上海交通大学 | 一种利用碱性标签和内含肽融合表达纯化重组蛋白的方法 |
| CN105925596A (zh) * | 2016-02-23 | 2016-09-07 | 上海交通大学 | 基于内含肽的药用重组蛋白的合成方法 |
| US11124539B2 (en) | 2016-11-16 | 2021-09-21 | Cytiva Bioprocess R&D Ab | Chromatography resin, production and use thereof |
-
2019
- 2019-11-22 GB GBGB1917046.3A patent/GB201917046D0/en not_active Ceased
-
2020
- 2020-11-20 JP JP2022526270A patent/JP7744026B2/ja active Active
- 2020-11-20 WO PCT/EP2020/082966 patent/WO2021099607A1/en not_active Ceased
- 2020-11-20 US US17/768,461 patent/US20240132538A1/en active Pending
- 2020-11-20 KR KR1020227016527A patent/KR20220105157A/ko active Pending
- 2020-11-20 EP EP20820794.4A patent/EP4061932A1/en active Pending
- 2020-11-20 CA CA3155170A patent/CA3155170A1/en active Pending
- 2020-11-20 CN CN202080080416.2A patent/CN114698379B/zh active Active
- 2020-11-20 CN CN202510113276.9A patent/CN120249241A/zh active Pending
-
2025
- 2025-06-17 JP JP2025101380A patent/JP2025133756A/ja active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014528720A (ja) | 2011-09-28 | 2014-10-30 | エラ、ビオテック、ソシエダッド、アノニマEra Biotech, S.A. | スプリットインテインおよびその使用 |
| JP2015522020A (ja) | 2012-06-27 | 2015-08-03 | ザ・トラスティーズ・オブ・プリンストン・ユニバーシティThe Trustees Of Princeton University | スプリットインテイン、複合体およびそれらの使用 |
| JP2016504417A (ja) | 2013-01-11 | 2016-02-12 | ザ テキサス エー アンド エム ユニヴァーシティー システムThe Texas A&M University System | インテイン媒介によるタンパク質の精製 |
| JP2016534373A (ja) | 2013-10-09 | 2016-11-04 | ピュリディファイ リミテッド | クロマトグラフィー担体 |
| JP2017533701A (ja) | 2014-11-03 | 2017-11-16 | メルク パテント ゲーエムベーハー | 可溶性インテイン融合タンパク質、及び生体分子を精製する方法 |
| JP2017537632A (ja) | 2014-12-17 | 2017-12-21 | ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ | 改変κ軽鎖結合ポリペプチド |
| US20160207965A1 (en) | 2015-01-09 | 2016-07-21 | Ohio State Innovation Foundation | Protein production systems and methods thereof |
| JP2019506163A (ja) | 2016-01-29 | 2019-03-07 | ザ・トラスティーズ・オブ・プリンストン・ユニバーシティThe Trustees Of Princeton University | 例外的なスプライシング活性を有するスプリットインテイン |
| JP2019524088A (ja) | 2016-07-06 | 2019-09-05 | アミコージェン・インコーポレイテッド | アルカリ耐性が向上した変異型免疫グロブリン結合タンパク質 |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20220105157A (ko) | 2022-07-26 |
| CN114698379B (zh) | 2024-12-27 |
| US20240132538A1 (en) | 2024-04-25 |
| JP2023502335A (ja) | 2023-01-24 |
| WO2021099607A1 (en) | 2021-05-27 |
| CN120249241A (zh) | 2025-07-04 |
| JP2025133756A (ja) | 2025-09-11 |
| EP4061932A1 (en) | 2022-09-28 |
| GB201917046D0 (en) | 2020-01-08 |
| CA3155170A1 (en) | 2021-05-27 |
| CN114698379A (zh) | 2022-07-01 |
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