CN114698379B - 使用断裂内含肽系统的蛋白质纯化 - Google Patents

使用断裂内含肽系统的蛋白质纯化 Download PDF

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Publication number
CN114698379B
CN114698379B CN202080080416.2A CN202080080416A CN114698379B CN 114698379 B CN114698379 B CN 114698379B CN 202080080416 A CN202080080416 A CN 202080080416A CN 114698379 B CN114698379 B CN 114698379B
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intein
variant
solid phase
protein
poi
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Chinese (zh)
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CN114698379A (zh
Inventor
C·J·塞文斯基
P·伦德贝克
J·奥曼
G·格罗斯曼
S·R·丁
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Cytiva Bioprocess R&D AB
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Cytiva Bioprocess R&D AB
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C07K17/02—Peptides being immobilised on, or in, an organic carrier
    • C07K17/10—Peptides being immobilised on, or in, an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
CN202080080416.2A 2019-11-22 2020-11-20 使用断裂内含肽系统的蛋白质纯化 Active CN114698379B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202510113276.9A CN120249241A (zh) 2019-11-22 2020-11-20 使用断裂内含肽系统的蛋白质纯化

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1917046.3 2019-11-22
GBGB1917046.3A GB201917046D0 (en) 2019-11-22 2019-11-22 Improved protein production
PCT/EP2020/082966 WO2021099607A1 (en) 2019-11-22 2020-11-20 Protein purification using a split intein system

Related Child Applications (1)

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CN202510113276.9A Division CN120249241A (zh) 2019-11-22 2020-11-20 使用断裂内含肽系统的蛋白质纯化

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CN114698379A CN114698379A (zh) 2022-07-01
CN114698379B true CN114698379B (zh) 2024-12-27

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CN202080080416.2A Active CN114698379B (zh) 2019-11-22 2020-11-20 使用断裂内含肽系统的蛋白质纯化
CN202510113276.9A Pending CN120249241A (zh) 2019-11-22 2020-11-20 使用断裂内含肽系统的蛋白质纯化

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Country Link
US (1) US20240132538A1 (https=)
EP (1) EP4061932A1 (https=)
JP (2) JP7744026B2 (https=)
KR (1) KR20220105157A (https=)
CN (2) CN114698379B (https=)
CA (1) CA3155170A1 (https=)
GB (1) GB201917046D0 (https=)
WO (1) WO2021099607A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4126899A1 (en) * 2020-03-25 2023-02-08 Merck Patent GmbH Reuse of intein-bound resins for protein purification
KR20240007210A (ko) 2021-05-12 2024-01-16 씨티바 바이오프로세스 알＆디 에이비 개선된 단백질 정제
CN114606252A (zh) * 2022-04-20 2022-06-10 广州市乾相生物科技有限公司 一种基于过滤法的寡肽合成及纯化方法
CN115028741A (zh) * 2022-06-21 2022-09-09 苏州工业园区唯可达生物科技有限公司 一种肿瘤抗原抗体复合物及制备方法和应用
CN115925830B (zh) * 2022-08-15 2023-11-07 广州市乾相生物科技有限公司 一种内含肽变体及其在生物法制备类蛇毒肽前体中的应用
AU2024270243A1 (en) 2023-05-08 2025-11-06 Cytiva Bioprocess R&D Ab Split inteins for affinity capture
EP4726045A1 (en) 2023-06-07 2026-04-15 Peking University Third Hospital (The Third Clinical Medical School of Peking University) Expression cassette combination and use thereof
EP4724477A1 (en) 2023-06-11 2026-04-15 Regeneron Pharmaceuticals, Inc. Circularized antibody molecules

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108884154A (zh) * 2016-01-29 2018-11-23 普林斯顿大学理事会 具有独特剪接活性的断裂内含肽

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102096534B1 (ko) * 2011-09-28 2020-04-03 에라 바이오테크, 에스.에이. 분할된 인테인 및 그의 이용
EP3431497B1 (en) 2012-06-27 2022-07-27 The Trustees of Princeton University Split inteins, conjugates and uses thereof
WO2014056199A1 (zh) * 2012-10-12 2014-04-17 清华大学 多肽的产生和纯化方法
US10087213B2 (en) 2013-01-11 2018-10-02 The Texas A&M University System Intein mediated purification of protein
WO2015052460A1 (en) 2013-10-09 2015-04-16 Ucl Business Plc Chromatography medium
PL3215614T3 (pl) 2014-11-03 2019-12-31 Merck Patent Gmbh Rozpuszczalne białka fuzyjne inteiny i sposoby oczyszczania biocząsteczek
JP7204086B2 (ja) 2014-12-17 2023-01-16 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 改変κ軽鎖結合ポリペプチド
US10066027B2 (en) 2015-01-09 2018-09-04 Ohio State Innovation Foundation Protein production systems and methods thereof
CN105316353A (zh) * 2015-02-13 2016-02-10 上海交通大学 一种利用碱性标签和内含肽融合表达纯化重组蛋白的方法
CN105925596A (zh) * 2016-02-23 2016-09-07 上海交通大学 基于内含肽的药用重组蛋白的合成方法
KR101857953B1 (ko) 2016-07-06 2018-05-16 아미코젠주식회사 알칼리 내성이 증가된 변이 면역글로불린 결합 단백질
US11124539B2 (en) 2016-11-16 2021-09-21 Cytiva Bioprocess R&D Ab Chromatography resin, production and use thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108884154A (zh) * 2016-01-29 2018-11-23 普林斯顿大学理事会 具有独特剪接活性的断裂内含肽

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Merideth Ann Cooper ET AL.Creating an Efficient Biopharmaceutical Factory: Protein Expression and Purification Using a Self-Cleaving Split Intein.《PHD THESIS ,Ohio State University》.2018,参见第113页最后1段以及第115页第1段. *

Also Published As

Publication number Publication date
KR20220105157A (ko) 2022-07-26
US20240132538A1 (en) 2024-04-25
JP2023502335A (ja) 2023-01-24
WO2021099607A1 (en) 2021-05-27
CN120249241A (zh) 2025-07-04
JP2025133756A (ja) 2025-09-11
EP4061932A1 (en) 2022-09-28
GB201917046D0 (en) 2020-01-08
CA3155170A1 (en) 2021-05-27
JP7744026B2 (ja) 2025-09-25
CN114698379A (zh) 2022-07-01

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