CA3155170A1 - Protein purification using a split intein system - Google Patents

Protein purification using a split intein system

Info

Publication number
CA3155170A1
CA3155170A1 CA3155170A CA3155170A CA3155170A1 CA 3155170 A1 CA3155170 A1 CA 3155170A1 CA 3155170 A CA3155170 A CA 3155170A CA 3155170 A CA3155170 A CA 3155170A CA 3155170 A1 CA3155170 A1 CA 3155170A1
Authority
CA
Canada
Prior art keywords
intein
taxon
protein
solid phase
poi
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3155170A
Other languages
English (en)
French (fr)
Inventor
Christopher James Sevinsky
Peter LUNDBACK
Johan Ohman
Gregory Grossmann
Sean R. DINN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Bioprocess R&D AB
Original Assignee
Cytiva Bioprocess R&D AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cytiva Bioprocess R&D AB filed Critical Cytiva Bioprocess R&D AB
Publication of CA3155170A1 publication Critical patent/CA3155170A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C07K17/02—Peptides being immobilised on, or in, an organic carrier
    • C07K17/10—Peptides being immobilised on, or in, an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
CA3155170A 2019-11-22 2020-11-20 Protein purification using a split intein system Pending CA3155170A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1917046.3 2019-11-22
GBGB1917046.3A GB201917046D0 (en) 2019-11-22 2019-11-22 Improved protein production
PCT/EP2020/082966 WO2021099607A1 (en) 2019-11-22 2020-11-20 Protein purification using a split intein system

Publications (1)

Publication Number Publication Date
CA3155170A1 true CA3155170A1 (en) 2021-05-27

Family

ID=69137378

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3155170A Pending CA3155170A1 (en) 2019-11-22 2020-11-20 Protein purification using a split intein system

Country Status (8)

Country Link
US (1) US20240132538A1 (https=)
EP (1) EP4061932A1 (https=)
JP (2) JP7744026B2 (https=)
KR (1) KR20220105157A (https=)
CN (2) CN114698379B (https=)
CA (1) CA3155170A1 (https=)
GB (1) GB201917046D0 (https=)
WO (1) WO2021099607A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4126899A1 (en) * 2020-03-25 2023-02-08 Merck Patent GmbH Reuse of intein-bound resins for protein purification
KR20240007210A (ko) 2021-05-12 2024-01-16 씨티바 바이오프로세스 알＆디 에이비 개선된 단백질 정제
CN114606252A (zh) * 2022-04-20 2022-06-10 广州市乾相生物科技有限公司 一种基于过滤法的寡肽合成及纯化方法
CN115028741A (zh) * 2022-06-21 2022-09-09 苏州工业园区唯可达生物科技有限公司 一种肿瘤抗原抗体复合物及制备方法和应用
CN115925830B (zh) * 2022-08-15 2023-11-07 广州市乾相生物科技有限公司 一种内含肽变体及其在生物法制备类蛇毒肽前体中的应用
AU2024270243A1 (en) 2023-05-08 2025-11-06 Cytiva Bioprocess R&D Ab Split inteins for affinity capture
EP4726045A1 (en) 2023-06-07 2026-04-15 Peking University Third Hospital (The Third Clinical Medical School of Peking University) Expression cassette combination and use thereof
EP4724477A1 (en) 2023-06-11 2026-04-15 Regeneron Pharmaceuticals, Inc. Circularized antibody molecules

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102096534B1 (ko) * 2011-09-28 2020-04-03 에라 바이오테크, 에스.에이. 분할된 인테인 및 그의 이용
EP3431497B1 (en) 2012-06-27 2022-07-27 The Trustees of Princeton University Split inteins, conjugates and uses thereof
WO2014056199A1 (zh) * 2012-10-12 2014-04-17 清华大学 多肽的产生和纯化方法
US10087213B2 (en) 2013-01-11 2018-10-02 The Texas A&M University System Intein mediated purification of protein
WO2015052460A1 (en) 2013-10-09 2015-04-16 Ucl Business Plc Chromatography medium
PL3215614T3 (pl) 2014-11-03 2019-12-31 Merck Patent Gmbh Rozpuszczalne białka fuzyjne inteiny i sposoby oczyszczania biocząsteczek
JP7204086B2 (ja) 2014-12-17 2023-01-16 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 改変κ軽鎖結合ポリペプチド
US10066027B2 (en) 2015-01-09 2018-09-04 Ohio State Innovation Foundation Protein production systems and methods thereof
CN105316353A (zh) * 2015-02-13 2016-02-10 上海交通大学 一种利用碱性标签和内含肽融合表达纯化重组蛋白的方法
PT3408292T (pt) 2016-01-29 2023-07-19 Univ Princeton Inteínas divididas com excecional atividade de splicing
CN105925596A (zh) * 2016-02-23 2016-09-07 上海交通大学 基于内含肽的药用重组蛋白的合成方法
KR101857953B1 (ko) 2016-07-06 2018-05-16 아미코젠주식회사 알칼리 내성이 증가된 변이 면역글로불린 결합 단백질
US11124539B2 (en) 2016-11-16 2021-09-21 Cytiva Bioprocess R&D Ab Chromatography resin, production and use thereof

Also Published As

Publication number Publication date
KR20220105157A (ko) 2022-07-26
CN114698379B (zh) 2024-12-27
US20240132538A1 (en) 2024-04-25
JP2023502335A (ja) 2023-01-24
WO2021099607A1 (en) 2021-05-27
CN120249241A (zh) 2025-07-04
JP2025133756A (ja) 2025-09-11
EP4061932A1 (en) 2022-09-28
GB201917046D0 (en) 2020-01-08
JP7744026B2 (ja) 2025-09-25
CN114698379A (zh) 2022-07-01

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