JP7727628B2 - 核酸結合免疫サンドイッチアッセイ（ｎｕｌｉｓａ） - Google Patents

核酸結合免疫サンドイッチアッセイ（ｎｕｌｉｓａ）

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Publication number
JP7727628B2
JP7727628B2 JP2022533354A JP2022533354A JP7727628B2 JP 7727628 B2 JP7727628 B2 JP 7727628B2 JP 2022533354 A JP2022533354 A JP 2022533354A JP 2022533354 A JP2022533354 A JP 2022533354A JP 7727628 B2 JP7727628 B2 JP 7727628B2
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nucleic acid
tag
target
fragment
binder
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JP2023505195A (ja
JP2023505195A5 (https=
Inventor
ルオ ユリン
フェン ウェイ
グルツィボウスキ エイドリアン
イン イユアン
チェン シーピン
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アラマー バイオサイエンシーズ， インク．
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C07K16/247—IL-4
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C07K16/248—IL-6
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/71—Assays involving receptors, cell surface antigens or cell surface determinants for growth factors; for growth regulators

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2022533354A 2019-12-03 2020-12-02 核酸結合免疫サンドイッチアッセイ（ｎｕｌｉｓａ） Active JP7727628B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025133045A JP2025166110A (ja) 2019-12-03 2025-08-08 核酸結合免疫サンドイッチアッセイ（ｎｕｌｉｓａ）

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962943135P 2019-12-03 2019-12-03
US62/943,135 2019-12-03
PCT/US2020/062791 WO2021113290A1 (en) 2019-12-03 2020-12-02 Nucleic acid linked immune-sandwich assay (nulisa)

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025133045A Division JP2025166110A (ja) 2019-12-03 2025-08-08 核酸結合免疫サンドイッチアッセイ（ｎｕｌｉｓａ）

Publications (3)

Publication Number Publication Date
JP2023505195A JP2023505195A (ja) 2023-02-08
JP2023505195A5 JP2023505195A5 (https=) 2023-12-13
JP7727628B2 true JP7727628B2 (ja) 2025-08-21

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JP2022533354A Active JP7727628B2 (ja) 2019-12-03 2020-12-02 核酸結合免疫サンドイッチアッセイ（ｎｕｌｉｓａ）
JP2025133045A Pending JP2025166110A (ja) 2019-12-03 2025-08-08 核酸結合免疫サンドイッチアッセイ（ｎｕｌｉｓａ）

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Country Status (5)

Country Link
US (3) US12105084B2 (https=)
EP (1) EP4069731A4 (https=)
JP (2) JP7727628B2 (https=)
CN (1) CN115066434A (https=)
WO (1) WO2021113290A1 (https=)

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US11566993B2 (en) * 2018-01-24 2023-01-31 University Of Connecticut Automated cell identification using shearing interferometry
US11461592B2 (en) 2018-08-10 2022-10-04 University Of Connecticut Methods and systems for object recognition in low illumination conditions
WO2020154307A1 (en) * 2019-01-22 2020-07-30 Singular Genomics Systems, Inc. Polynucleotide barcodes for multiplexed proteomics
CN115380218A (zh) 2019-10-02 2022-11-22 诺米克生物公司 用于多重化的连接-共定位夹心测定
GB202004469D0 (en) 2020-03-27 2020-05-13 Olink Proteomics Ab Controls for proximity detection assays
US20220390442A1 (en) * 2021-06-02 2022-12-08 Alamar Biosciences, Inc. Nucleic acid linked immune-sandwich assay (nulisa)
CN116256506B (zh) * 2021-12-10 2026-03-24 深圳先进技术研究院 基于磁球分离时间分辨荧光的抗原检测的曲线建立方法、检测方法及其试剂盒
CN114292899B (zh) * 2022-01-08 2024-03-26 安徽医科大学第一附属医院 一种肝细胞生长因子检测及信号增敏探针、引物对及其应用
CN121548832A (zh) * 2023-05-10 2026-02-17 阿拉玛生物科学公司 全自动生化测定仪器系统
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WO2025111398A1 (en) 2023-11-20 2025-05-30 Alamar Biosciences, Inc. Reduction of non-cognate signal in multiplex proximity ligation assays
WO2025111396A1 (en) 2023-11-20 2025-05-30 Alamar Biosciences, Inc. Molecular barcode set and use thereof in multiplex proximity detection assays
WO2025252658A1 (en) 2024-06-03 2025-12-11 Olink Proteomics Ab New product and method
WO2026074476A1 (en) * 2024-10-02 2026-04-09 Csl Behring Ag Method of detecting autoantibodies against fc epsilon receptor i

Citations (1)

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US20180292394A1 (en) 2016-04-11 2018-10-11 Joshua Caine Soldo Sample depletion and enrichment to improve the quality of diagnostic test results

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