JP7032930B2 - 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ - Google Patents

核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ Download PDF

Info

Publication number
JP7032930B2
JP7032930B2 JP2017524429A JP2017524429A JP7032930B2 JP 7032930 B2 JP7032930 B2 JP 7032930B2 JP 2017524429 A JP2017524429 A JP 2017524429A JP 2017524429 A JP2017524429 A JP 2017524429A JP 7032930 B2 JP7032930 B2 JP 7032930B2
Authority
JP
Japan
Prior art keywords
capture
primer
wells
primer pair
immobilized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017524429A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017533710A5 (cg-RX-API-DMAC7.html
JP2017533710A (ja
Inventor
エル ガンダーソン ケヴィン
バイ ジンウェイ
ウィリアム ケリンジャー マシュー
エム バイアール ジョン
マーク バウテル ジョナサン
リガッティ ロベルト
キャンデラリア ロジャート バシガルポ マリア
ボヤノフ ボヤン
マイジンガー クラウス
Original Assignee
イルミナ ケンブリッジ リミテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by イルミナ ケンブリッジ リミテッド filed Critical イルミナ ケンブリッジ リミテッド
Publication of JP2017533710A publication Critical patent/JP2017533710A/ja
Publication of JP2017533710A5 publication Critical patent/JP2017533710A5/ja
Priority to JP2021213441A priority Critical patent/JP7323598B2/ja
Application granted granted Critical
Publication of JP7032930B2 publication Critical patent/JP7032930B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277Apparatus
    • B01J2219/00279Features relating to reactor vessels
    • B01J2219/00306Reactor vessels in a multiple arrangement
    • B01J2219/00308Reactor vessels in a multiple arrangement interchangeably mounted in racks or blocks
    • B01J2219/0031Reactor vessels in a multiple arrangement interchangeably mounted in racks or blocks the racks or blocks being mounted in stacked arrangements
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277Apparatus
    • B01J2219/00279Features relating to reactor vessels
    • B01J2219/00306Reactor vessels in a multiple arrangement
    • B01J2219/00313Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315Microtiter plates
    • B01J2219/00317Microwell devices, i.e. having large numbers of wells
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583Features relative to the processes being carried out
    • B01J2219/00585Parallel processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583Features relative to the processes being carried out
    • B01J2219/00596Solid-phase processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583Features relative to the processes being carried out
    • B01J2219/00603Making arrays on substantially continuous surfaces
    • B01J2219/00605Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608DNA chips
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583Features relative to the processes being carried out
    • B01J2219/00603Making arrays on substantially continuous surfaces
    • B01J2219/00605Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632Introduction of reactive groups to the surface
    • B01J2219/00637Introduction of reactive groups to the surface by coating it with another layer
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718Type of compounds synthesised
    • B01J2219/0072Organic compounds
    • B01J2219/00722Nucleotides
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0829Multi-well plates; Microtitration plates
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877Flow chambers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50Detection characterised by immobilisation to a surface
    • C12Q2565/513Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50Detection characterised by immobilisation to a surface
    • C12Q2565/543Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Structural Engineering (AREA)
  • Dispersion Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2017524429A 2014-11-11 2015-11-11 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ Active JP7032930B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2021213441A JP7323598B2 (ja) 2014-11-11 2021-12-27 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201462078346P 2014-11-11 2014-11-11
US62/078,346 2014-11-11
US201462096464P 2014-12-23 2014-12-23
US62/096,464 2014-12-23
PCT/EP2015/076353 WO2016075204A1 (en) 2014-11-11 2015-11-11 Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2021213441A Division JP7323598B2 (ja) 2014-11-11 2021-12-27 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ

Publications (3)

Publication Number Publication Date
JP2017533710A JP2017533710A (ja) 2017-11-16
JP2017533710A5 JP2017533710A5 (cg-RX-API-DMAC7.html) 2018-12-20
JP7032930B2 true JP7032930B2 (ja) 2022-03-09

Family

ID=54540094

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2017524429A Active JP7032930B2 (ja) 2014-11-11 2015-11-11 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ
JP2021213441A Active JP7323598B2 (ja) 2014-11-11 2021-12-27 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2021213441A Active JP7323598B2 (ja) 2014-11-11 2021-12-27 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ

Country Status (13)

Country Link
US (3) US10619204B2 (cg-RX-API-DMAC7.html)
EP (1) EP3218511B1 (cg-RX-API-DMAC7.html)
JP (2) JP7032930B2 (cg-RX-API-DMAC7.html)
KR (1) KR102590448B1 (cg-RX-API-DMAC7.html)
CN (1) CN107208019B (cg-RX-API-DMAC7.html)
AU (1) AU2015345125B2 (cg-RX-API-DMAC7.html)
DK (1) DK3218511T3 (cg-RX-API-DMAC7.html)
ES (1) ES2802405T3 (cg-RX-API-DMAC7.html)
MY (1) MY182476A (cg-RX-API-DMAC7.html)
PL (1) PL3218511T3 (cg-RX-API-DMAC7.html)
PT (1) PT3218511T (cg-RX-API-DMAC7.html)
SG (2) SG10202004350TA (cg-RX-API-DMAC7.html)
WO (1) WO2016075204A1 (cg-RX-API-DMAC7.html)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL3246416T3 (pl) 2011-04-15 2024-09-30 The Johns Hopkins University Bezpieczny system sekwencjonowania
CA2889937C (en) 2012-10-29 2020-12-29 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
HK1221747A1 (zh) 2013-07-30 2017-06-09 哈佛学院院长及董事 基於dna的定量成像和超分辨成像
US10415083B2 (en) 2013-10-28 2019-09-17 The Translational Genomics Research Institute Long insert-based whole genome sequencing
KR102590448B1 (ko) 2014-11-11 2023-10-17 일루미나 케임브리지 리미티드 핵산의 모노클로날 클러스터를 형성시키고 시퀀싱하기 위한 방법 및 어레이
WO2016168825A1 (en) * 2015-04-17 2016-10-20 Centrillion Technology Holdings Corporation Methods for performing spatial profiling of biological molecules
US11092606B2 (en) * 2015-08-07 2021-08-17 President And Fellows Of Harvard College Super resolution imaging of protein-protein interactions
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
JP2017209051A (ja) * 2016-05-25 2017-11-30 株式会社ニコン 標的生体分子の検出方法、標的生体分子検出用基板、及び標的生体分子検出装置
AU2017379872B2 (en) * 2016-12-22 2024-08-29 Illumina, Inc. Arrays with quality control tracers
GB201704754D0 (en) * 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
NL2019044B1 (en) 2017-05-11 2018-11-15 Illumina Inc Protective surface coatings for flow cells
WO2018218136A1 (en) * 2017-05-25 2018-11-29 Integrated Dna Technologies, Inc. Reverse complement adapters for the mitigation of umi hopping
US11001885B2 (en) 2017-06-13 2021-05-11 Personal Genomics Taiwan, Inc. Apparatus for single molecular sequencing and method of sequencing nucleic acid molecules
WO2019067092A1 (en) 2017-08-07 2019-04-04 The Johns Hopkins University METHODS AND SUBSTANCES FOR THE EVALUATION AND TREATMENT OF CANCER
CN107523640A (zh) * 2017-10-12 2017-12-29 厦门燕旭安生物科技有限公司 一种ctDNA精准测序的扩增子文库构建方法
RU2751517C2 (ru) * 2017-12-21 2021-07-14 Иллюмина, Инк. Проточные ячейки с покрытием из гидрогеля
WO2019228995A1 (en) * 2018-05-28 2019-12-05 F. Hoffmann-La Roche Ag Enzymatic enrichment of dna-pore-polymerase complexes
WO2020005503A1 (en) * 2018-06-29 2020-01-02 Illumina, Inc. Flow cells
TWI730352B (zh) 2018-08-06 2021-06-11 英商伊路米納劍橋有限公司 流體槽與定序系統及製備用於其之基板的方法
US20200149095A1 (en) * 2018-11-14 2020-05-14 Element Biosciences, Inc. Low binding supports for improved solid-phase dna hybridization and amplification
WO2020114918A1 (en) * 2018-12-05 2020-06-11 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification
SG11202012807YA (en) 2018-12-18 2021-01-28 Illumina Cambridge Ltd Methods and compositions for paired end sequencing using a single surface primer
WO2020132103A1 (en) * 2018-12-19 2020-06-25 Illumina, Inc. Methods for improving polynucleotide cluster clonality priority
TWI873119B (zh) 2019-01-29 2025-02-21 美商伊路米納有限公司 流通槽及製備其之方法
TWI857000B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 流體槽以及將複合物引入至流體槽之方法
TWI857001B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 定序套組
CN112654719A (zh) * 2019-05-31 2021-04-13 伊鲁米纳公司 使用流动池进行信息存储和检索的系统和方法
US20220230706A1 (en) 2019-05-31 2022-07-21 Nec Corporation Information processing apparatus, information processing method and information processing program
TW202120693A (zh) * 2019-08-21 2021-06-01 美商伊路米納有限公司 偵測核苷酸之聚合酶併入的方法
PH12021552315A1 (en) * 2019-12-16 2022-07-04 Illumina Cambridge Ltd Kits and flow cells
TW202130416A (zh) 2019-12-20 2021-08-16 美商伊路米納有限公司 流動池
JP2023508799A (ja) * 2019-12-23 2023-03-06 イルミナ インコーポレイテッド 鋳型ポリヌクレオチド付着のための単一部位を有するナノ粒子
US20220073977A1 (en) 2020-02-14 2022-03-10 The Johns Hopkins University Methods and materials for assessing nucleic acids
WO2021168097A1 (en) * 2020-02-19 2021-08-26 Genapsys, Inc. Methods and systems for nucleic acid processing
CN114144529B (zh) * 2020-02-26 2025-04-29 因美纳有限公司 用于基因分型的试剂盒
CA3173798A1 (en) * 2020-03-03 2021-09-10 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
CA3145466A1 (en) * 2020-03-09 2021-09-16 Illumina, Inc. Improvements in nucleic acid sequencing
US12209266B2 (en) 2020-05-12 2025-01-28 Illumina, Inc. Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
WO2022055729A1 (en) * 2020-09-14 2022-03-17 Illumina, Inc. Compositions and methods for amplifying polynucleotides
JP2023544469A (ja) * 2020-09-29 2023-10-24 イルミナ インコーポレイテッド フローセル及びそれを作製するための方法
US20220097048A1 (en) * 2020-09-29 2022-03-31 Illumina, Inc. Methods for making flow cells
KR20230118501A (ko) * 2020-12-10 2023-08-11 일루미나, 인코포레이티드 이미지 센서 구조 및 관련된 방법
US11884977B2 (en) 2021-03-12 2024-01-30 Singular Genomics Systems, Inc. Nanoarrays and methods of use thereof
EP4263868A4 (en) * 2021-03-12 2024-11-27 Singular Genomics Systems, Inc. NANONETWORKS AND METHODS OF USE THEREOF
EP4330428A1 (en) 2021-04-30 2024-03-06 Illumina Cambridge Limited Flow cell and methods
EP4337786A1 (en) * 2021-05-10 2024-03-20 Pacific Biosciences of California, Inc. Single-molecule seeding and amplification on a surface
WO2024006625A1 (en) * 2022-06-27 2024-01-04 Somalogic Operating Co., Inc. Monoclonal polony generation using nucleic acid supramolecular structures
EP4547387A1 (en) * 2022-06-29 2025-05-07 10X Genomics, Inc. Compositions and methods for oligonucleotide inversion on arrays
CN116024320A (zh) * 2022-07-13 2023-04-28 上海翔琼生物技术有限公司 一种用于检测核酸的荧光定量pcr方法
WO2024057089A1 (en) * 2022-09-09 2024-03-21 Todx Inc. Device for parallel detection and quantification of nucleic acid based markers
JP2025532733A (ja) * 2022-09-16 2025-10-03 イルミナ インコーポレイテッド ポリヌクレオチド結合部位を有するナノ粒子及びそれを作製する方法
WO2024256580A1 (en) * 2023-06-14 2024-12-19 Illumina, Inc. Concurrent sequencing with spatially separated rings
WO2025062001A1 (en) * 2023-09-20 2025-03-27 Illumina, Inc. Optimised nucleic acid sequencing
WO2025128699A1 (en) 2023-12-12 2025-06-19 Illumina, Inc. Capturing and amplifying polynucleotides using molecules and particles
US20250207193A1 (en) * 2023-12-22 2025-06-26 Illumina, Inc. Reusable flow cells and methods of using them for nucleic acid sequencing
CN117778578B (zh) * 2023-12-29 2024-10-15 深圳海普洛斯医学检验实验室 一种基于高通量测序检测目标基因甲基化程度的引物组合物及其应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008157640A2 (en) 2007-06-18 2008-12-24 Illumina, Inc. Microfabrication methods for the optimal patterning of substrates
WO2013063382A2 (en) 2011-10-28 2013-05-02 Illumina, Inc. Microarray fabrication system and method
US20130338042A1 (en) 2012-06-15 2013-12-19 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US20140079923A1 (en) 2012-06-08 2014-03-20 Wayne N. George Polymer coatings
WO2014133905A1 (en) 2013-02-26 2014-09-04 Illumina, Inc. Gel patterned surfaces

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
WO1998044151A1 (en) 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
EP1368460B1 (en) 2000-07-07 2007-10-31 Visigen Biotechnologies, Inc. Real-time sequence determination
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
WO2004018497A2 (en) 2002-08-23 2004-03-04 Solexa Limited Modified nucleotides for polynucleotide sequencing
DE60324810D1 (de) 2002-09-20 2009-01-02 New England Biolabs Inc HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
ES2949821T3 (es) 2004-01-07 2023-10-03 Illumina Cambridge Ltd Matrices moleculares
AU2005296200B2 (en) 2004-09-17 2011-07-14 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
US7682816B2 (en) * 2005-04-07 2010-03-23 454 Life Sciences Corporation Thin film coated microwell arrays and methods of using same
JP4990886B2 (ja) 2005-05-10 2012-08-01 ソレックサ リミテッド 改良ポリメラーゼ
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
JP5122555B2 (ja) 2006-03-31 2013-01-16 ソレクサ・インコーポレイテッド 合成解析による配列決定システムおよび装置
EP2089517A4 (en) 2006-10-23 2010-10-20 Pacific Biosciences California POLYMERASEENZYME AND REAGENTS FOR ADVANCED NUCKIC ACID SEQUENCING
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP3285067B1 (en) 2006-12-14 2022-06-22 Life Technologies Corporation Apparatus for measuring analytes using fet arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8728764B2 (en) 2008-10-02 2014-05-20 Illumina Cambridge Limited Nucleic acid sample enrichment for sequencing applications
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
CA2770389C (en) 2009-08-25 2015-12-29 Illumina, Inc. Methods for selecting and amplifying polynucleotides
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
PL3290528T3 (pl) 2011-09-23 2020-03-31 Illumina, Inc. Sposoby i kompozycje do sekwencjonowania kwasów nukleinowych
IN2014DN07992A (cg-RX-API-DMAC7.html) 2012-04-03 2015-05-01 Illumina Inc
WO2015095291A1 (en) * 2013-12-19 2015-06-25 Illumina, Inc. Substrates comprising nano-patterning surfaces and methods of preparing thereof
BR112016016546B1 (pt) 2014-01-16 2022-09-06 Illumina, Inc Método de preparação de amplicon e métodos para determinação da presença de um gene associado a câncer e de uma variante de sequência de ácidos nucleicos verdadeira
KR102590448B1 (ko) 2014-11-11 2023-10-17 일루미나 케임브리지 리미티드 핵산의 모노클로날 클러스터를 형성시키고 시퀀싱하기 위한 방법 및 어레이

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008157640A2 (en) 2007-06-18 2008-12-24 Illumina, Inc. Microfabrication methods for the optimal patterning of substrates
WO2013063382A2 (en) 2011-10-28 2013-05-02 Illumina, Inc. Microarray fabrication system and method
US20140079923A1 (en) 2012-06-08 2014-03-20 Wayne N. George Polymer coatings
US20130338042A1 (en) 2012-06-15 2013-12-19 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
WO2014133905A1 (en) 2013-02-26 2014-09-04 Illumina, Inc. Gel patterned surfaces

Also Published As

Publication number Publication date
JP7323598B2 (ja) 2023-08-08
KR102590448B1 (ko) 2023-10-17
KR20170098823A (ko) 2017-08-30
SG10202004350TA (en) 2020-06-29
ES2802405T3 (es) 2021-01-19
JP2022050504A (ja) 2022-03-30
US20200318182A1 (en) 2020-10-08
MY182476A (en) 2021-01-25
CN107208019A (zh) 2017-09-26
PT3218511T (pt) 2020-07-23
CA2967525A1 (en) 2016-05-19
AU2015345125B2 (en) 2022-03-17
PL3218511T3 (pl) 2020-10-19
HK1244845A1 (en) 2018-08-17
EP3218511A1 (en) 2017-09-20
US20230340593A1 (en) 2023-10-26
CN107208019B (zh) 2021-01-01
EP3218511B1 (en) 2020-04-22
US10619204B2 (en) 2020-04-14
US20180037950A1 (en) 2018-02-08
DK3218511T3 (da) 2020-07-20
WO2016075204A1 (en) 2016-05-19
AU2015345125A1 (en) 2017-05-25
JP2017533710A (ja) 2017-11-16
US11692223B2 (en) 2023-07-04
SG11201703693UA (en) 2017-06-29
US12385092B2 (en) 2025-08-12

Similar Documents

Publication Publication Date Title
JP7032930B2 (ja) 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ
US9677132B2 (en) Polynucleotide modification on solid support
US9944924B2 (en) Polynucleotide modification on solid support
US12123056B2 (en) Surface concatemerization of templates
EP2943589B1 (en) Methods and solid supports for sample preparation using transposomes
EP3303621A1 (en) Surface-based tagmentation
JP6716465B2 (ja) 集積した単一細胞の配列決定
KR20180039585A (ko) 클러스터에서 표면 프라이머의 향상된 이용
US20220154173A1 (en) Compositions and Methods for Preparing Nucleic Acid Sequencing Libraries Using CRISPR/CAS9 Immobilized on a Solid Support
CA2967525C (en) Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
HK1244845B (en) Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
HK1231514B (en) Polynucleotide modification on solid support

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170707

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180523

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20181109

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20181109

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191217

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200317

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200515

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20201027

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210126

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210326

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210421

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20210831

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7423

Effective date: 20211206

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20211206

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20211227

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20211227

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20220111

C21 Notice of transfer of a case for reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C21

Effective date: 20220118

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20220201

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20220225

R150 Certificate of patent or registration of utility model

Ref document number: 7032930

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250